Page 1
User’s Guide
EN
G
Page 2
NOTICE
TABLE OF CONTENTS
INTRODUCTION | 3
Parts Checklist | 3
Good Laboratory Practice (GLP) Compliance | 4
Description | 5
Specifications | 6
SETTING THE VOLUME | 8
PIPETTING | 9
Fitting the Tips | 9
TABLE OF CONTENTS
Pre-Wetting the Tips | 11
Aspirating | 11
Dispensing | 12
Guidelines for Good Pipetting | 12
ACCESSORIES | 14
TROUBLESHOOTING | 15
Leak Test | 16
MAINTENANCE | 17
Maintenance for Single Channel Models | 17
Maintenance for Multichannel Models | 21
CLEANING AND DECONTAMINATION | 21
SPARE PARTS | 25
WARRANTY | 28
Decreased pipetting forces are due
to the redesigned piston assembly
including the use of a very high
quality lubricants. The use of other
lubricant cancels the warranty
of this pipette. The approved
lubricant is available under the
part number 5440011070. Please
contact your Gilson distributor.
2
TABLE OF CONTENTS | PIPETMAN® G
Page 3
Chapter 1
NOTICE
INTRODUCTION
PIPETMAN® G is a fully adjustable air-displacement
pipette and is used with disposable tips.
To meet pipette user’s needs, PIPETMAN G relies
on the legendary accuracy, precision, and reliability
of PIPETMAN, with pipetting and purge forces
dramatically decreased for hours of comfortable
pipetting.
Eight single channel models cover a volume
range from 0.2 µL to 10 mL.
● P2G and P10G: measurement and transfer
of microliter volumes, DNA sequencing, and
enzyme assays
● P20G, P100G, P200G, and P1000G:
measurement and transfer of general
aqueous solutions, acids, and bases
● P5000G and P10mLG: measurement and
transfer of large volumes
Eight multichannel models cover a volume range
from 1 µL to 300 µL.
PIPETMAN G is identified by a visual
blue ring.
Parts Checklist
After unpacking the pipette, verify that the
following items were included and are undamaged:
Single Models
● PIPETMAN G
● PIPETMAN G Quick Guide
● Safety bag
● Dual-position adapter (for P2G and P10G
models only)
● Certificate of Conformity (including
barcode sticker)
Multichannel Models
● PIPETMAN G
● PIPETMAN G Quick Guide
● Safety bag
● Ejector spacers for D10 tips (for PX10G
model only)
● Certificate of Conformity (including
barcode sticker)
INTRODUCTION
PIPETMAN® G | INTRODUCTION
3
Page 4
Good Laboratory Practice (GLP)
Compliance
The serial number is engraved on the body of the
pipette. It provides unique identification of your
pipette and the date of manufacture.
Ex: AA10369
The barcode on the box and the Certificate of
Conformity provide traceability of your pipette.
INTRODUCTION
Serial number
Figure 1
Serial number location
4
INTRODUCTION | PIPETMAN® G
Page 5
Description
Color-coded
push button
Tip ejector button
Thumbwheel
Blue ring
Body or
handle
Clip tip ejector
Connecting nut
Ejector support
Ejector lock
Cover
Tip ejector
Tip holder
Ejector spacer
PIPETMAN®
DIAMOND tip
Figure 2
PIPETMAN® G multichannel and single channel models
INTRODUCTION
Please refer to the following chapters for a full
description of the different parts and functions of
the pipette.
PIPETMAN® G | USER’S GUIDE
5
Page 6
Specifications
NOTICE
PIPETMAN G is a high quality pipette that offers
excellent accuracy and precision. The data provided
in the Gilson Maximum Permissible Errors table
were obtained using PIPETMAN® DIAMOND Tips.
These values are guaranteed only when genuine
PIPETMAN DIAMOND Tips are used.
Each pipette is inspected and validated by qualified
technicians in accordance with the Gilson Quality
System. Gilson declares that its manufactured
pipettes comply with the requirements of
the ISO 8655 standard by type testing.
SPECIFICATIONS
The adjustment is carried out under strictly
defined and monitored conditions (ISO 8655-6).
The data given in the tables conform
to the ISO 8655-2 standard.
With a precise pipetting technique,
the P2G model may be used to
aspirate volumes as low as 0.1 µL and
the P10G model as low as 0.5 µL .
PIPETMAN G is equipped with a stainless steel
tip ejector and can also be used with a plastic tip
ejector, which is available as an accessory (refer
to ACCESSORIES on page 14).
6
INTRODUCTION | PIPETMAN® G
Page 7
Maximum Permissible Errors
MAXIMUM PERMISSIBLE ERRORS
VOLUM E
(µL)
PIPE TMAN G SI NGLE CH ANNE L
P2G (P/N F1 44054 M) WITH D10 O R DL10 TIP S
0.2
0.5
1
2
P10G (P/N F 14405 5M) WITH D1 0 OR DL10 TI PS
1
5
10
P20G (P/N F 14405 6M) WITH D2 00 TIPS
2
10
20
P100G ( P/N F1440 57M) WITH D2 00 TIPS
10
50
100
P200G ( P/N F1440 58M) WIT H D200 TI PS
20
100
200
P100 0G (P/N F144 059M) WI TH D100 0 TIPS
100
500
1000
P5000 G* (P/N F144 066) WI TH D500 0 TIPS
500
2500
5000
P10M LG* (P/N F14 4067) WI TH D10M L TIPS
1000
5000
10000
PIPETMAN G MULTICHANNEL
P8X10 G (P/N F1440 68) P12X10G (P/N F 144069)
WITH D10 A ND DL10 TI PS
1
5
10
P8X20 G (P/N F14407 0) P12X2 0G (P/N F14 4071)
WITH DL1 0 TIPS
2
10
20
P8X20 0G (P/N F14 4072) P12 X200G ( P/N F14407 3)
WITH D20 0 TIPS
20
100
200
P8X300 G (P/N F144 074) P12X300 G (P/N F144 075)
WITH D30 0 TIPS
30
150
300
* P5000G and P10mLG do not have tip ejectors
GILSON ISO 865 5
SYSTEMATIC
ERROR
(µL)
± 0.024
± 0.025
± 0.025
± 0.030
± 0.025
± 0.075
± 0.100
± 0.10
± 0.10
± 0.20
± 0.35
± 0.40
± 0.80
± 0.50
± 0.80
± 1.60
± 3.0
± 4.0
± 8.0
± 12
± 15
± 30
± 30
± 40
± 60
± 0.08
± 0.20
± 0.20
± 0.10
± 0.20
± 0.40
± 0.50
± 1.00
± 2.00
± 1.00
± 1.50
± 3.00
RANDOM
ERROR
(µL)
≤ 0.012
≤ 0.012
≤ 0.012
≤ 0.014
≤ 0.012
≤ 0.030
≤ 0.040
≤ 0.030
≤ 0.050
≤ 0.060
≤ 0.10
≤ 0.12
≤ 0.15
≤ 0.20
≤ 0.25
≤ 0.30
≤ 0.6
≤ 1.0
≤ 1.5
≤ 3
≤ 5
≤ 8
≤ 6
≤ 10
≤ 16
≤ 0.05
≤ 0.10
≤ 0.10
≤ 0.08
≤ 0.10
≤ 0.20
≤ 0.25
≤ 0.40
≤ 0.50
≤ 0.35
≤ 0.60
≤ 1.00
SYSTEMATIC
ERROR
(µL)
± 0.08
± 0.08
± 0.08
± 0.08
± 0.12
± 0.12
± 0.12
± 0.2
± 0.2
± 0.2
± 0.8
± 0.8
± 0.8
± 1.6
± 1.6
± 1.6
± 8.0
± 8.0
± 8.0
± 40
± 40
± 40
± 60
± 60
± 60
± 0.24
± 0.24
± 0.24
± 0.4
± 0.4
± 0.4
± 3.2
± 3.2
± 3.2
± 8.0
± 8.0
± 8.0
PIPETMAN® G | USER’S GUIDE
RANDOM
ERROR
(µL)
≤ 0.04
≤ 0.04
≤ 0.04
≤ 0.04
≤ 0.08
≤ 0.08
≤ 0.08
≤ 0.1
≤ 0.1
≤ 0.1
≤ 0.3
≤ 0.3
≤ 0.3
≤ 0.6
≤ 0.6
≤ 0.6
≤ 3.0
≤ 3.0
≤ 3.0
≤ 15.0
≤ 15.0
≤ 15.0
≤ 30.0
≤ 30.0
≤ 30.0
≤ 0.16
≤ 0.16
≤ 0.16
≤ 0.20
≤ 0.20
≤ 0.20
≤ 1.2
≤ 1.2
≤ 1.2
≤ 3.0
≤ 3.0
≤ 3.0
SPECIFICATIONS
7
Page 8
Chapter 2
Px10G
Px20G
Chapter 2
SETTING THE VOLUME
The volume of liquid to be aspirated is set using
the volumeter. The dials are colored either black or
red to indicate the position of the decimal point,
depending on the model (refer to Figure 3).
P2G
P10G
The volume is set by
turning the thumbwheel
or the push button
(refer to Figure 4). The
push button makes it
easier and quicker to
1.25 µL
P100G
75 µL
set volumes, especially
when wearing gloves.
SETTING THE VOLUME
The thumbwheel may
be turned using only one
hand to slowly reach the
Figure 3
Dial colors by model
required setting.
MODEL
P2G µL 0.01 µL 0.002 µL
P10G - P20G - PX10G - PX20G µL 0.1 µL 0.02 µL
P100G - P200G - PX200G - PX300G µL - 0.2 µL
P1000G 0.01 mL mL 0.002 mL
P5000G 0.01 mL mL 0.002 mL
P10mLG mL 0.1 mL 0.02 mL
COLOR OF VOLUMETER NUMBERS
BLAC K RED INCREMENT
1
2
5
0
7
5
P5000G
1
2
5
1.25 mL
7.5 µL
P200G
125 µL
0
7
5
1
2
5
P20G
12.5 µL
P1000G
0.75 mL
P10mLG
0
7
5
7.5 mL
1
2
0
7
5
7.5 µL
Px200G
1
2
5
125 µL
1
2
5
12.5 µL
Px300G
1
2
5
125 µL
5
0
7
5
To achieve maximum accuracy when setting the
volume, proceed as follows:
● When decreasing
the volume setting,
slowly reach the
required setting,
making sure to not
overshoot the mark.
● When increasing
the volume setting,
pass the required
value by 1/3 of
a turn and then
slowly decrease to
reach the volume,
making sure to not
overshoot the mark.
8
SETTING THE VOLUM E | PIPETMAN® G
Push button
Thumbwheel
Figure 4
Location of
push button and
thumbwheel
Page 9
Chapter 3
NOTE
NOTE
PIPETTING
While many brands of tips can be used with
the PIPETMAN G, it is recommended to use
PIPETMAN DIAMOND Tips with the PIPETMAN G
for optimum performance. These tips are made
from pure polypropylene. Plastic tips are for a
single application—they should not be cleaned for
reuse.
Fitting the Tips
To fit a new PIPETMAN DIAMOND Tip, push the tip
holder into the tip using a slight twisting motion to
ensure a firm, airtight seal.
Fitting the Tips on P2G and P10G Models
For the P2G and P10G models, a dualposition adapter (plastic) is required
to fit DL10 tips (longtips) or D10 tips
(short tips). The metallic rod of the tip
ejector is shaped so the adapter may
be clipped to it in e it he r position.
P2G and P10G models are
delivered with the adapter
in place, ready to use
DL10 tips. If D10 tips are
used, the adapter must be
repositioned in the shorter
slot as follows:
1. Pull the adapter down
from the metallic rod.
2. Turn the adapter 180°.
3. Refit the adapter so the
end of the metallic rod
engages the shorter slot
of the adapter.
The dual-position
adapter is
autoclavable.
Short tips
Figure 5
Dual-position
adapter for P2G
and P10G with
stainless steel tip
ejector
Long tips
PIPETTING
PIPETMAN® G | USER’S GUIDE
9
Page 10
An autoclavable tip ejector extension can
be ordered as an accessory for P2 and P10
equipped with a plastic tip ejector (part
number F107027; refer to ACCESSORIES
on page 14).
To fit a tip ejector extension:
1. Slide the extension over the tip holder.
2. Push the extension firmly onto the
end of the tip ejector until it clicks
into place.
PIPETTING
To remove a tip ejector extension:
1. Gently twist the extension.
2. Pull it away from the pipette.
Ejector Spacer for PIPETMAN G 10 µL
Multichannel Models
According to the tip used, D10 or DL10, you may
have to exchange the ejector spacer. The broad
one is dedicated to D10, and the small one is
dedicated to DL10.
● Remove the tip ejector, keeping both ejector
locks depressed. Pull the tip ejector down.
● Gently press the
tabs from the ejector
spacer and remove it
from the tip ejector.
● Insert the alternative
ejector spacer and
click it to the tip
ejector.
● To refit the tip ejector,
gently re-insert the tip
ejector vertically into
the rails of the ejector support.
spacer
Ejector
Figure 6
Ejector spacer location
Ejector spacer
D10
Short tipsLong tips
PIPETMAN DIAMOND Tips are best fitted with
the ROCK Y RACK technique, invented by Gilson,
available only in our TIPACK and TOWERPACK.
PIPETMAN DIAMOND Tip Compatibility
for Multichannel Models
Figure 7
ROCKY RACK
technique
10
PIPETTING | PIPETMAN® G
P8x10G, P12x10G D10*, DL10, DF10, DFL10
P8x20G, P12x20G DL10, DFL10, DF30, D200
P8x200G, P12x200G D200, D300, DF200, DF300
P8x300G, P12x300G D200, D300, DF200, DF300
* Using the broad ejector spacer D10, you can adapt a
D10 tip (see above).
Figure 8
PIPETMAN DIAMOND Tips
compatibility charts for
multichannel models
Page 11
Pre-Wetting the Tips
Pre-wetting the tips before pipetting helps
prepare the tips for the best pipetting
performance. Ideally, the pre-wet includes
both immersing the tip in the liquid and
performing one pipetting step.
Pre-wetting the tips helps ensure that volumes
that you pipette will achieve accuracy and
precision within specifications.
1
2
Aspirating
1. Press the push button to the first stop
(this corresponds to the set volume of liquid).
2. Hold the pipette vertically and immerse the
tip in the liquid (refer to Guideline for Good
Pipetting on page 12).
3. Release the push button slowly and smoothly
(to the top position) to aspirate the set
volume of liquid. Wait one second (refer to
Guidelines for Good Pipetting on page 12)
and then withdraw the pipette-tip from the
liquid.
4. You may wipe any droplets away from the
outside of the tip using a medical wipe,
however, if you do so take care to avoid
touching the tip’s orifice.
PIPETTING
Rest position
First stop
Second stop
Figure 9
Pipetting motion - aspirate and dispense
PIPETMAN® G® | USER’S GUIDE
11
Page 12
Dispensing
1. Place the end of the tip against the inside wall of
the recipient vessel (at an angle of 10° to 40°).
2. Press the push button slowly and smoothly
to the first stop.
3. Wait for at least one second, then press the
push button to the second stop to expel
any residual liquid from the tip. Keep the
push button pressed fully down and (while
PIPETTING
removing the pipette) draw the tip along the
inside surface of the vessel.
4. Release the push button smoothly. Eject the tip
by pressing firmly on the tip ejector button.
Guidelines for Good Pipetting
Make sure that you operate the push button slowly
and smoothly.
When aspirating, keep the tip at a constant depth
below the surface of the liquid (refer to the table
below).
IMMERSIO N DEPT H AND WAIT TIME
MODEL
P2G 1 1
P10G - Px10G 1 1
P20G - Px20G 2-3 1
P100G - Px200G - Px300G 2-4 1
P200G 2-4
P1000G 2-4
P5000G 3-6
P10mLG 5-7 4-5
IMMERSION
DEPTH (MM)
WAIT TIME
(SECONDS)
1
2-3
4-5
12
PIPETTING | PPIPETMAN® G
Page 13
● Change the tip before aspirating a different
NOTICE
liquid, sample, or reagent.
● Change the tip if a droplet remains at the end of
the tip from the previous pipetting operation.
● Pre-wet each new tip with the liquid to be
pipetted
● Liquid should never enter the tip holder.
To prevent this:
○ Press and release the push button slowly
and smoothly.
○ Never turn the pipette upside down.
○ Never lay the pipette on its side when
there is liquid in the tip.
● If you use the same tip with a larger volume,
pre-rinse the tip.
● For volatile solvents, you should saturate the
air cushion of your pipette by aspirating and
dispensing the solvent repeatedly before
aspirating the sample.
● When the temperature of the liquid is
different from the ambient temperature,
pre-wet the tip several times before use.
● You may remove the tip ejector (refer to
MAINTENANCE on page 17) to aspirate from
very narrow tubes.
● After pipetting acids or other corrosive liquids
that emit vapors, remove the tip ejector
and the tip holder; and then rinse, dry, and
lubricate the piston (refer to MAINTENANCE
on page 17). For the model P1000G, you can
increase the lifetime of the piston by using a
specific tip holder equipped with a filter (refer
to ACCESSORIES on page 14).
● Do not pipette liquids with temperatures
above 70°C or below 4°C. The pipette
can be used between 4°and 40°C, but the
specifications may vary according to the
temperature (refer to Maximum Permissible
Errors on page 7).
PIPETTING
PIPETTING
It is recommended to regularly verify
the volume displayed on the dial
while pipetting.
PIPETMAN® G | USER’S GUIDE
13
13PIPETTING | PPIPETMAN® G
Page 14
Chapter 4
ACCESSORIES
To make pipetting more comfortable and
more secure, Gilson has developed several
accessories.
DESCRIPTION PART NUMBER
Plastic ejector P2/P10 with adapter F107027
Plastic ejector P20 F107028
Plastic ejector P100 F107029
ACCESSORIES
Plastic ejector P200 F107030
Plastic ejector P1000 F107031
Pipette stands allow users to store pipettes
vertically to avoid the possibility of liquid
running back into the pipette.
DESCRIPTION PART NUMBER
CARROUSEL™ Pipet te Stand ( 7 pipet tes) F161401
TRIO™ Stand (3 pipet tes) F161405
SINGLE™ Pip ette Holder F16140 6
COLORIS™ Identification Clips identify or personalize
your pipette. Write your name or application to avoid
mix-ups and then snap into place.
DESCRIPTION PART NUMBER
COLORIS clips (mixed colors set of 10) F161301
COLORIS clips (red, set of 10)
COLORIS clips (ye llow, set of 1 0) F1 61303
COLORIS clips (green , set of 10) F161304
COLORIS clips (blue, s et of 10) F161305
COLORIS clips (white, set of 1 0) F161306
F161302
With The JIMMY ™, hands-free microtube opener, you
can open both snap-cap and screw-cap microtubes
to increase pipetting efficiency and reduce the
fatigue associated with manual tube opening.
DESCRIPTION PART NUMBER
THE JIMMY (set of 3) F144983
To protect the piston when pipetting corrosive
liquids, you can use a specific tip holder and
filter for the model P1000G.
DESCRIPTION PART NUMBER
Corrosion Protection Kit
(tip holder + a bag of 10 filte rs)
14
ACCESSORIES | PIPETMAN® G
F144570
Page 15
Chapter 5
NOTE
TROUBLESHOOTING
A quick inspection of the pipette may help you to
detect a problem.
You can download the Two Minute
Inspection Poster from the Gilson
website (www.gilson.com). The poster
describes how to perform a quick
diagnosis of your pipette.
The following table can help you to identify and
correct the problem you might encounter.
SYM PTOM POSSIBLE CAUSE ACTION
SINGLE CHANNEL
Pipette is leaking
sample
Pipette won’t
aspirate
Pipette is
inaccurate
Pipette is not
precise
Tips fall o or
do not fit
correctly
Pipetting seize up Piston need lubricant Lubricate piston assembly
MULTICHANNEL MODELS
Tips fall o or do
not fit correctly
Pipette won’t
aspirate
Pipette is
inaccurate
Pipette is not
precise
If you can not solve the prob lem, contact y our local Gils on representa tive.
Damaged tip holder
Worn O-ring or seal
Worn O-ring and seal
Damaged tip holder
Connecting nut is loose
Damaged or corroded piston
Improper repair or assembly
Improper repair or assembly
Unscrewed tip holder
Connecting nut is loose
Tip holder is loose
Connecting nut is loose
Incorrect operator technique
Damaged or corroded piston(s)
Damaged tip holder(s)
Worn O-ring or seal
Low quality tips
Dirty tip holder
Damaged tip holder(s)
Damaged tip ejector
Ejector spacer is missing
The ejector spacer is damaged
The tip ejector is loose
The ejector lock is misaligned
Low quality tips
Damaged tip ejector
Damaged ejector spacer
Dirty tip holder(s)
Damaged tip holder
Connecting nut is loose Tighten the connecting nut
Connecting nut is loose Tighten the connecting nut
Connecting nut is loose
Incorrect operator technique
Replace the tip holder
Replace both parts and lubricate
Replace both parts and lubricate
Replace the tip holder
Tighten connecting nut
Return pipette to supplier (refer to
MAINTENANCE on page 17)
Refer to page 17 - MAINTENANCE
Tighten connecting nut
Tighten connecting nut
Tighten connecting nut
Tighten connecting nut
Operator training
Return pipette to supplier
Replace the tip holder
Replace both parts and lubricate
Use PIPETMAN DIAMOND Tips
Clean the tip holder with alcohol
Replace the tip holder
Replace tip ejector
Mount the spacer on the tip ejector
Replace the ejector spacer
Assemble the tip ejector properly
Align the ejector lock
Use PIPETMAN DIAMOND Tips
Replace the tip ejector
Replace the ejector spacer
Clean the tip holder(s) with
isopropanol or ethanol
Contact your local authorized Gilson
Service Center
Tighten the connecting nut
Operator training
TROUBLESHOOTING
PIPETMAN® G | USER’S GUIDE
15
Page 16
CAUTION
Before returning any pipette to
your local Gilson Service Center,
ensure that it is completely free of
chemical, biological, or radioactive
contamination (refer to CLEANING
AND DECONTAMINATION on page
22). Please use the included safety
bag to return the pipette to your
local Gilson Service Center.
Leak Test
This test may be performed at any time to check
that the pipette does not leak, especially after
performing a maintenance or decontamination
procedure. If a pipette fails this test, replace the
TROUBLESHOOTING
O-ring and seal. After making sure that the pipette
is correctly reassembled, repeat this test.
For the P2G to P200G Models
1. Fit a PIPETMAN DIAMOND Tip.
2. Set the pipette to the maximum volume
given in the specifications and pre-wet.
3. Aspirate the set volume from a beaker of
dist il led water.
4. Maintain the pipette in the vertical position
and wait for 20 seconds.
● If a water droplet appears at the end of
the tip, there is a leak.
● If you see no droplet, re-immerse the tip
below the surface of water.
The water level inside the tip should
remain constant. If the level goes down,
there is a leak.
For the P1000G, P5000G, and P10mLG
Models
1. Fit a PIPETMAN DIAMOND Tip.
2. Set the pipette to the maximum volume given
in the specifications.
3. Aspirate the set volume from a beaker of
dist il led water.
4. Maintain the pipette in the vertical position and
wait for 20 seconds.
● If a water droplet appears at the end of
the tip, there is a leak.
TROUBLESHOOTING | PIPETMAN® G
16
Page 17
NOTICE
For the Multichannel Models (PX8G PX12G)
1. Fit the PIPETMAN DIAMOND Tip.
2. Set the pipette to the maximum volume
given in the specifications and pre-wet.
3. Aspirate the set volume from a reagent
reservoir of distilled water.
4. Maintain the pipette in the vertical position
and wait for 20 seconds. Fluid level in the
tips should remain constant.
● If a water droplet appears at the end of
the tip, there is a leak.
● For volumes below 200 μL, if you see no
droplets, re-immerse the tip below the
surface of water. The water level inside
the tip should remain constant. If the
level goes down, there is a leak.
Chapter 6
MAINTENANCE
Routine maintenance will help keep your pipette
in good condition, ensuring a continued high level
of performance.
Maintenance is limited to:
● Cleaning or decontamination (refer to
CLEANING AND DECONTAMINATION on
page 22).
● Replacing spare parts
● Greasing the piston assembly
MAINTENANCE
MAINTENANCE
Maintenance for Single Channel
Models
PIPETMAN P2G and P10G should not be
disassembled, so you can only replace the push
button, tip ejector, and its dual position adapter.
With these pipettes if the tip holder is damaged,
the piston may also be damaged.
After replacing any parts, you should
verify the performance of your
pipette following the verification
procedure available on the Gilson
website (www.gilson.com). If the
pipette needs to be readjusted,
please contact your local Gilson
Service Center.
PIPETMAN® G | USER’S GUIDE
17
Page 18
Changing the Tip Ejector for Single
Channel Models
To remove:
1
Push the ejection button.
Laterally push the tip ejector.
2
Slide and remove the
3
tip eje ctor.
To r efit :
Push the ejection
1
MAINTENANCE
button.
Slide the tip ejector
2
along the tip holder.
Clip the tip ejector on
3
the body of the pipette.
Changing the Tip Holder – No Tools
Required
1. Remove the tip ejector (refer to Changing
the Tip Ejector for Single Channel Models
on page 18).
2. Unscrew the connecting nut by turning it
counterclockwise.
3. Carefully separate the lower and upper parts.
4. Remove the piston assembly and the seals.
5. Clean, autoclave, or replace the tip holder.
6. If necessary, lightly lubricate the piston
and its seals (refer to Servicing the Piston
Assembly on page 19).
7. Reassemble the pipette (refer to the Figure 10
on page 19).
8. Tighten the connecting nut (turn clockwise)
9. Refit the tip ejector (refer to Changing the Tip
Ejector for Single Channel Models on page 18).
1
2
3
MAINTENANCE | PIPETMAN® G
18
Page 19
NOTICE
P10G, P20G
P100G, P200G
Tip holder
P1000G
Color-coded
push button
Blue ring
Connecting nut
Piston
assembly
P5000G
P10mLG
Tip ejector
MAINTENANCE
Stainless
steel
Plastic tip ejector
available as an
acessory
Figure 10
Disassembled PIPETMAN G, with dif ferent piston
assemblies
Servicing the Piston Assembly
You may remove the piston assembly for cleaning
purposes only. If the piston assembly is changed,
the pipette must be adjusted and calibrated at
a local Gilson Service Center. Because P2G and
P10G models contain miniaturized parts, it is best
not to disassemble these pipettes yourself.
The piston assembly must not be
autoclaved.
PIPETMAN® G | USER’S GUIDE
19
Page 20
1. Remove the tip ejector (refer to
NOTICE
Changing the Tip Ejector for Single
Channel Models on page 18).
2. Unscrew the connecting nut by
turning it counterclockwise.
3. Carefully separate the lower and
upper parts.
4. Remove the piston assembly and
the seals.
5. Leave the piston exposed, clean it
with isopropanol or ethanol, and
lubricate lightly. For P100G, P200G,
MAINTENANCE
and P1000G models, lubricate only the internal
part of the piston and the seals.
For P5000G and P10mLG, lubricate the tip
of the piston, disassemble the seals, and
lubricate their internal part.
6. Reassemble the pipette (refer to Figure 10 on
page 19).
7. Tighten the connecting nut (turn clockwise).
8. Refit the tip ejector (refer to Changing the Tip
Ejector for Single Channel Models on page 18).
Changing the Seal Guide (or Seal)
The O-ring and seal on the piston must not be
autoclaved. If worn or damaged in any way (chemical
or mechanical), they must be replaced. Because the
models P2G and P10G contain miniaturized parts, it
is best not to disassemble these pipettes yourself.
Please contact your local Gilson Service Center.
The dimensions of the O-ring vary depending on the
model of pipette.
1. Remove the tip ejector (refer to Changing the Tip
Ejector for Single Channel Models on page 18).
2. Unscrew the connecting nut by turning it
counterclockwise.
3. Carefully separate the lower and upper parts.
4. Remove the piston assembly, O-ring, and seal.
5. If necessary, clean the piston and replace the seal.
Lightly lubricate both seal and piston. Remember
to replace the seal and O-ring in the correct order.
6. Reassemble the pipette (refer to the Figure 10
on page 18).
7. Tighten the connecting nut (turn clockwise).
8. Refit the tip ejector (refer to Changing the Tip
Ejector for Single Channel Models on page 18).
MAINTENANCE | PIPETMAN® G
20
Decreased pipetting forces are due to
the design of the piston assembly, which
includes a very high-quality lubricant (part
number 5440011070). The use of any other
lubricant voids the warranty of this pipette.
Page 21
Maintenance for Multichannel
Models
Routine maintenance will help keep your pipette
in good condition, ensuring a continued high
level of performance.
Maintenance is limited to:
● Cleaning or decontamination (refer to
CLEANING AND DECONTAMINATION on
page 22)
● Replacing spare parts
● Greasing the piston assembly
Changing the Tip Ejector for
Mutichannel Model
To remove the tip ejector, keep both ejector
locks depressed. Pull the tip ejector down.
To refit the tip ejector, gently re-insert the tip
ejector vertically into the rails of the ejector
support. Pull lightly on the tip ejector to check
the position.
MAINTENANCE
Tip ejector
Ejector lock
Ejector spacer
Figure 11
PIPETMAN® G multichannel tip ejector and ejector spacers
PIPETMAN® G | USER’S GUIDE
21
Page 22
Chapter 7
NOTICE
CLEANING AND
DECONTAMINATION
PIPETMAN G is designed so that the parts
normally in contact with liquid contaminants can
easily be cleaned and decontaminated. However,
because the models P2G and P10G contain
miniaturized parts, it is best not to disassemble
these pipettes yourself. Please contact your local
Gilson Service Center.
You can refer to the
decontamination procedure
available on the Gilson website
(www.gilson.com). Liquid must
never enter the upper part
(handle) of any pipette.
Cleaning for Single Channel Models
The pipette must be cleaned, as described below,
before it is decontaminated. Soap solution is
CLEANING AND DECONTAMINATION
recommended for cleaning PIPETMAN G.
External
1. Remove the tip ejector (refer to Changing the Tip
Ejector for Single Channel Models on page 18).
2. Wipe the tip ejector with a soft cloth or lint-free
tissue soaked with soap solution.
3. Wipe the entire pipette with a soft cloth or
lint-free tissue soaked with soap solution to
remove all dirty marks. If the pipette is very dirty,
a brush with soft plastic bristles may be used.
4. Wipe the entire pipette and the tip ejector
with a soft cloth or lint-free tissue soaked with
dist il led water.
5. Refit the tip ejector and allow the pipette to dry.
Internal
Only the following components can be immersed
in a cleaning solution: connecting nut, tip ejector,
tip holder, piston assembly, seal, and O-ring.
1. Disassemble the pipette (refer to Figure 10 on
page 19).
2. Set aside the upper part in a clean, dry place.
Clean the individual components of the lower
part of the pipette using an ultrasonic bath
(20 minutes at 50°C) or with a soft cloth and
brushes.
CLEANING AND DECONTAMINATION | PIPETMAN® G
22
Page 23
NOTE
The piston assembly and seals must
be degreased with isopropanol or
ethanol before being immersed in the
ultrasonic bath. Small round brushes
with sof t plastic bristles may be used
to clean the interior of the tip holder.
3. Rinse the individual components with distilled water.
4. Let the parts dry by evaporation or wipe them
with a clean soft cloth or lint-free tissue.
5. Reassemble the pipette as described (refer to
Figure 10 on page 19).
Cleaning for Multichannel Models
Only the following components can be immersed
in a cleaning solution: tip ejector, ejector locks, and
ejector spacer.
1. Remove the tip ejector and the ejector spacer.
2. Immerse the tip ejector, ejector locks, and
ejector spacer in the cleaning solution or wipe
them with a soft cloth or lint-free tissue soaked
with the cleaning solution.
3. Rinse the components with distilled water.
4. Wipe the entire pipette with a soft cloth
or lint-free tissue soaked with the cleaning
solution.
5. Wipe it with distilled water.
6. Let the parts dry by evaporation or wipe them
with a clean soft cloth or lint-free tissue.
7. Refit the tip ejector (refer to Changing the Tip
Ejector for Multichannel Models on page 21).
CLEANING AND DECONTAMINATION
Autoclaving
The upper part (body) and the piston assembly of
the pipette are not autoclavable. Only the following
parts may be autoclaved: tip ejector, tip holder,
and connecting nut. The O-ring and seal are not
autoclavable. They may be cleaned or replaced with
the ones found in SPARE PARTS on page 25.
1. Clean the parts to be autoclaved, especially
the tip holder.
2. Put the parts in an autoclaving sack.
3. Autoclave for 20 minutes at 121°C, 0.1 MPa.
4. Check that the parts are dry before
reassembling the pipette.
5. Set the pipette aside to equilibrate to room
temperature.
6. Reassemble the pipette (refer to Figure 10 on
page 19).
PIPETMAN® G | USER’S GUIDE
23
Page 24
Chemical Decontamination
You may choose to decontaminate your pipette
chemically, in accordance with your own procedures.
Whatever decontaminant you use, check with the
supplier of the decontaminant to verify that it is
compatible with stainless steel and the plastics used
in the construction of the pipette: PA (Polyamide),
PBT (Polybutylene Terephtalate), PC (Polycarbonate),
PC/PBT (Polycarbonate/Polybutylene Terephtalate),
POM (Polyoxymethylene), or PVDF (Polyvinylidene
Fluoride).
Chemical Decontamination for Single
Channel Models
Upper Part (handle)
1. Wipe the upper part (handle) of the pipette
with a soft cloth or lint-free tissue soaked
with the chosen decontaminant.
2. Wipe the upper part of the pipette with
a soft cloth or lint-free tissue soaked with
distilled water or sterile water.
Lower Part (volumetric module)
CLEANING AND DECONTAMINATION
Only the following components can be immersed
in a decontaminant solution: connecting nut, tip
ejector, and tip holder.
The piston assembly and seals must
be degreased with isopropanol or
NOTE
1. Remove the tip ejector, the tip holder, and the
connecting nut.
2. Immerse them in the decontamination solution.
3. Degrease the piston assembly and the seals.
Then immerse them in another vessel of
decontamination solution.
4. Rinse each component with distilled water.
5. Let the parts dry by evaporation. The tip
ejector, tip holder, and connecting nut can be
wiped dry with a soft cloth.
6. Lubricate the piston assembly and the seals.
7. Reassemble the piston assembly, the tip
holder, and the tip ejector.
ethanol in a separate vessel before
being immersed in decontamination
solution.
Chemical Decontamination for
Multichannel Models
Only the following components be immersed in a
decontamination solution: tip ejector, ejector locks,
and ejector spacer.
24
CLEANING AND DECONTAMINATION | PIPETMAN® G
Page 25
1. Remove the tip ejector and the ejector spacer.
2. Immerse the tip ejector, ejector locks and
ejector spacer in the decontamination solution
or wipe them with a soft cloth or lint-free tissue
soaked with the decontamination solution.
3. Rinse the components with distilled water.
4. Wipe the entire pipette with a soft cloth or lint-free
tissue soaked with the decontamination solution.
5. Wipe the entire pipette with distilled water.
6. Let the parts dry by evaporation or wipe them
with a clean soft cloth or lint-free tissue.
7. Refit the tip ejector (refer to Changing the Tip
Ejector for Multichannel Models, page 21).
Chapter 8
SPARE PARTS
Service Kit 1
st
level includes:
● 3 piston seals* or
seal guides
● 3 O-rings*
● 1 tip holder
C
D
E
Service Kit 2nd level includes:
● 1 push button
● 1 connecting nut
A
B
● Stainless steel
tip ejector
F2
● Plastic tip ejector F1
available separately as
an accessory
For the P2G and P10G
models only:
● 5 dual-position adapter for
stainless steel tip ejector
● 1 extension for plastic
tip ejector
F4
F3
A
B
C
D
SPARE PARTS
* For P50 00G, t wo piston
seals an d O-rings are
provide d on the Ser vice
Kit 1st level.
F4
Figure 12
Spare parts identified
F2
F3
F1
PIPETMAN® G | USER’S GUIDE
F2
E
25
Page 26
Single Channel Models
P2G (F 144054M) AND P1 0G (F 144055M)
LABEL DESCRIPTION
C+D+E Service Kit 1st level F144501 FA07001
A+B+F1 to F4 Service Kit 2nd level F1619702 F1619712
C+D
F3
SPARE PARTS
F4 Tip ejector extension F2070903 F2070903
P20G ( F14 4056 M) AND P 100 G (F14 4057M)
LABEL DESCRIPTION
C+D+E Service Kit 1st level FA07002 FA07003
A+B+F Service Kit 2nd level F1619722 F1619732
C+D
P200 G (F14405 8M) AND P1000G ( F14 4059 M)
LABEL
LABEL DESCRIPTION
C+D+E Service Kit 1st level FA07004 FA07005
A+B+F Service Kit 2nd level F1619742 F1619782
C+D
Seal Guide + O-ring
(5 sets)
Dual-Position Adapter
(x5)
Seal Guide + O-ring
(5 sets)
Seal Guide + O-ring
(5 sets)
P/N F OR
P2G
F144861 FA07012
F144879 F144879
P/N F OR
P20G
FA07013 FA07014
P/N F OR
P200G
FA07015 FA07016
P/N F OR
P10G
P/N F OR
P100G
P/N F OR
P1000G
P500 0G (F 144066) AND P10MLG (F 144067)
LABEL DESCRIPTION
C+D+E Service Kit 1st level FA07021 FA07022
C+D
E Tip holder F123608 F161263
DESCRIPTION PART NUMBER
Lubricant 5440011070
SPARE PART S | PIPETMAN® G
26
Seal Guide + O-ring
(2 sets)
P/N F OR
P5000G
FA07017 FA07018
P/N F OR
P10MLG
Page 27
Multichannel Models
F4
Tip ejector
(12 channels)
Tip ejector
(8 channels)
F3
Ejector lock
G
SPARE PARTS
Ejector spacer
(8 channels)
Ejector spacer D10
H4
(12 channels)
Ejector spacer
H2
(12 channels)
H3
Ejector spacer D10
(8 channels)
P8X1 0G (F1440 68) AN D P12X10G( F14 4069)
LABEL DESCRIPTION
F3-F4 Tip ejector F507005 F507006
G Ejector lock F507008 F507008
H1 - H2 Ejector spacer F507001 F507003
H3 - H4 Ejector spacer D10 F807114 F807115
P/N F OR
P8X10G
P/N F OR
P12X10G
P8X20G (F1 44070) AN D P12 X20G(F14 4071)
LABEL DESCRIPTION
F3-F4 Tip ejector F507005 F507006
G Ejector lock F507008 F507008
H1 - H2 Ejector spacer F507001 F507003
P/N F OR
P8X20G
P/N F OR
P12X20G
P8X200G (F 144 072) AND P 12X 200G(F14 4073)
LABEL DESCRIPTION
F3-F4 Tip ejector F507005 F507006
G Ejector lock F507008 F507008
H1 - H2 Ejector spacer F507001 F507003
P/N F OR
P8X200 G
P/N F OR
P12X200G
H1
P8X30 0G (F 144074) AND P12X300 G(F14 4075)
LABEL DESCRIPTION
F3-F4 Tip ejector F507005 F507006
G Ejector lock F507008 F507008
H1-H2 Ejector spacer F507001 F507003
P/N F OR
P8X300G
P/N F OR
P12X300G
PIPETMAN ® G | USER ’S GUIDE
27
Page 28
Chapter 9
NOTICE
WARRANTY
Gilson warrants this pipette against defects
in material under normal use and service for a
period of three years for both single channel
and multichannel models from the date of
purchase.
This warranty shall not apply to pipettes which
WARRANTY
are subject to abnormal use and/or improper
or inadequate maintenance (contrary to the
recommendations given in the user’s guide),
including, but not limited to pipettes which have
been subjected to physical damage, improper
handling, or spillage or exposure to any corrosive
environment. This warranty shall also be void
in the event pipettes are altered or modified by
any party other than Gilson or its designates.
Gilson’s sole liability under this warranty shall
be limited to, at Gilson’s sole option, repair or
replacement of any defective components of
pipettes or refund of the purchase price paid
for such pipettes. Routine cleaning, control,
and recalibration are not covered under the
warranty. The replacement of wearing parts such
as seals, O-rings, broken pistons assembly, and
broken tip holders are not covered under the
warranty.
THE FOREGOING WARRANTY IS EXCLUSIVE
AND GILSON HEREBY DISCLAIMS ALL OTHER
WARRANTIES, WHETHER EXPRESS OR
IMPLIED, INCLUDING ANY WARRANTIES OF
MERCHANTABILITY AND ANY WARRANTIES OF
FITNESS FOR A PARTICULAR PURPOSE, UNDER
NO CIRCUMSTANCES SHALL GILSON BE LIABLE
FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT
OR INCIDENTAL DAMAGES ARISING OUT OF
ANY BREACH OF ANY EXPRESS OR IMPLIED
WARRANTY.
28
WARRANTY | PIPETMAN® G
Yearly rou tine ma inten ance is highly
recommended to keep your pipette in
good condition, ensuring a continued
high level of performance.
Page 29
NOTES
NOTES
PIPETMAN® G | USER’S GUIDE
29
Page 30
gilson.com/contactus
LT801122/F | ©2021 Gilson, Inc. All rights reser ved.
Loading...