Gilson Extractman User Manual

User’s Guide

®

Gilson, Inc. | PO Box 620027 | Middleton, WI 53562-0027 | Tel: [phone] OR 800-445-7661 | Fax: [phone]

Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 Villiers-le-bel, FRANCE

www.gilson.com | [e-mail] | [e-mail] | [e-mail]

LT310210-01

1 Description

EXTRACTMAN® leverages proprietary Exclusion-based Sample Preparation
(ESP™) technology to gently and quickly target proteins from a variety of
samples, including weakly-bound protein complexes that traditional isolation
techniques leave behind. The sliding handle processes up to four samples
simultaneously in as few as 30 seconds.

Handle

Slides to advance the bead capture strip

SLIDE

ReleaseMagnet

Disengages the

capturemagnets to release

paramagneticbeads

4-RowMicroplate

Holds sample solutions

and reagents; wellmoats

collect spillover

ReleaseMagnet

Position Indicator

Displays the position

of the releasemagnet

Base

Positions the
4-rowmicroplate
and guides
the handle

ReleaseMagnet
Positioning Handle

Slides forward or
backward to position
the releasemagnet

Description

Figure 1: EXTRACTMAN® Component Descriptions

EXTRACTMAN® | User’s Guide

7

Input, Wash, and Output Volumes

NOTE

NOTE

● The rst wellcolumn (460–500 µL) was designed for input wells.

● The second (230–275 µL) and third (100–110 µL) wellcolumns were
designed for input or wash wells.

● The fourth and fth (100–110 µL) wellcolumns were designed for
wash wells.

● The sixth (10–15 µL) wellcolumn was designed for output wells.

Well volumes may vary because the formation
of stable, prominent menisci may require the
addition or subtraction of reagent, depending on
the surface tension of the reagent used.

Operation

Input
(Blue)

460–500 µL

Figure 10: Input, Wash, and Output Well Locations

Input or Wash

(Purple)

230–275 µL

Wash
(Red)

100–110 µL

Output
(Green)

10–15 µL

3. Fill each subsequent well with wash or reagent solution.

Wells should have prominent menisci to ensure proper
contact with the bead capture strip.

Figure 11: Prominent Menisci on the 4-RowMicroplate

EXTRACTMAN® | User’s GuideOperation | EXTRACTMAN®

17

Bead Size and Optimal Operation

The size of paramagnetic beads aects their time to pellet, suspension
dynamics, and performance.

Operation

Adjust Protocols for Large Paramagnetic Beads

Larger paramagnetic beads exert greater magnetic force than smaller
beads, resulting in a faster time to pellet. This dynamic may cause larger
paramagnetic beads to pull o the bead capture strip prematurely. This
can be avoided by raising the height of the bead capture strip. Refer to

Adjust the Collection Height on page 22.

Adjust Protocols for Small Paramagnetic Beads

Smaller paramagnetic beads exert less magnetic force than larger beads,
resulting in a slower time to pellet. This dynamic may result in longer
capture times. Gilson recommends waiting a few moments after the bead
discoloration in the wells has cleared before advancing to the next well
column.

Operation | EXTRACTMAN®

26

Figure 22: Magnetic Intensity and Paramagnetic Bead Size

TIME TO PELLET

Figure 23: Paramagnetic Bead Size and Time to Pellet