Gilson DISTRIMAN User Manual

User’s Guide
EN
DISTRIMAN® | USERS GUIDE
TABLES OF CONTENTS
DISTRIMAN® | USERS GUIDE
TABLE OF CONTENTS | DISTRIMAN®
TABLE OF CONTENTS
OPERATION | 4
Description | 4
Select the DISTRITIP® | 5
Fit the DISTRITIP® | 6
Select the Volume Range | 7
Set the Aliquot Volume | 8
Set the Volume | 8
Lock the Selected Volume | 9
Fill the Syringe | 9
Dispense the Aliquots | 10
RECOMMENDATIONS | 11
SPECIFICATIONS | 12
MAINTENANCE | 14
Replace the Push Button Assembly | 14
Replace the DISTRITIP® retaining spring | 15
Replace the Piston Retaining Assembly | 16
Replace the Red Flag | 16
Replace the COLORISTM | 17
DECONTAMINATION | 17
SPARE PARTS | 18
WARRANTY | 19
DISTRIMAN® | USER’S GUIDE
INTRODUCTION
3
DISTRIMAN® | USER’S GUIDE
Chapter 1
INTRODUCTION
DISTRIMAN® is a continuously adjustable repeater pipette designed to dispense precise aliquots of liquid safely. The DISTRIMAN can quickly transfer volumes from 1 µL to 1.25 mL, depending on the capacity of the DISTRITIP® (positive displacement syringe) fitted to it. The number of aliquots that can be dispensed depends on the volume of liquid aspirated (up to the maximum for a given size of syringe) and the aliquot volume selected.
This ergonomically designed product is suitable for transferring most kinds of liquids (aqueous, volatile, viscous, or dense) into many kinds of vials (tubes, microplates, etc.). DISTRIMAN ensures reliable results in any domain and in all kinds of laboratory, for a wide variety of applications.
The positive displacement mechanism permits precise pipetting of viscous materials (e.g. blood, plasma, oils, etc.), including those used in molecular biology (glycerol, enzymes, and amplified DNA), without contaminating the pipette.
DISTRIMAN is fitted with a COLORIS™ tag, which can identify the user or application. You may replace the one fitted with a COLORIS of a dierent color (as supplied, refer to Chapter 5
Maintenance on page 14).
DISTRIMAN is equipped with a direct reading volumeter that allows precise and continuous volume setting. The aliquots are dispensed by pressing the push button the required number of times (the volume between aliquots can also be changed). A small red flag (refer to Figure 1 on page 4) indicates that the final aliquot will be dispensed.
Because DISTRIMAN is permanently calibrated when manufactured, there is no need for readjustment. It is virtually maintenance free. However, a few spare parts have been made available in case they are damaged as the result of extensive use.
OPERATION
4
DISTRIMAN® | USERS GUIDE
OPERATION | DISTRIMAN®
Push Button Unit Indicator
Adjustment Ring Selector Switch
Parallax-limiting
Red Flag
Digital Volumeter
Filling Knob DISTRITIPS
Chapter 2
OPERATION
Description
Figure 1
DISTRIMAN Description
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OPERATION
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DISTRIMAN® | USER’S GUIDE
For example, the DISTRITIP Micro is ideal for filling a 96-well microplate. The syringe capacity is 125 µL, so the DISTRIMAN can dispense at least
96 aliquots of 1 µL.
Number of aliquots** =
Volume in Syringe
Volume of Aliquot
MODEL VOLUME
DISTRITIP Micro
125 µL
1 µL to 12.5 µL
DISTRITIP Mini
1250 µL
10 µL to 125 µL
DISTRITIP Maxi
12.5 mL
100 µL to 1250 µL
Table 1
DISTRTIPS Model
Perform the following sequence of steps in order to operate the DISTRIMAN®.
1. Select a DISTRITIP of suitable capacity.
2. Fit the DISTRITIP to the DISTRIMAN.
3. Select the volume range on the DISTRIMAN.
4. Set the aliquot volume.
5. Lock in the selected volume.
6. Fill the DISTRITIP.
7. Dispense the aliquots.
8. Eject the DISTRITIP.
Select the DISTRITIP
Three standard DISTRITIPS are provided with DISTRIMAN (refer to Table 1 below). Individually wrapped sterilized versions are available.
Select the capacity* of the DISTRITIP according to the volume and number of aliquots that you want to dispense.
Because DISTRIMAN is continuously adjustable you can also select any aliquot volume within the range for the size of DISTRITIP fitted.
OPERATION
6
DISTRIMAN® | USERS GUIDE
OPERATION | DISTRIMAN®
Fit the DISTRITIP
Take the following steps to ensure the DISTRITIP is correctly fitted.
1. Push the filling knob downwards to its lowest possible position, using your thumb . Hold the filling knob firmly in place with your thumb.
2. Hold the DISTRITIP in your other hand, place the end of the piston into the body of the DISTRIMAN at a right-angles to the main axis .
3. Gently rotate the DISTRITIP downwards with the other hand , and then snap the syringe into place with your thumb, using moderate pressure .
4. Finally, check that the piston of the syringe is correctly fitted by pushing the filling knob
upwards.
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Figure 2
Fit the DISTRITIP
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NOTE
Before using DISTRIMAN, check the selected volume range matches the
volume of the DISTRITIP.
NOTE
*Capacity refers to the maximum usable volume of a DISTRITIP; the range refers to the maximum and minimum aliquot volumes that may be dispensed.
**Taking into account the systematic error, the maximum number of aliquots varies from 118 to 121, according to the type of DISTRITIP used.
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