ASPEC® Positive Pressure Manifold
User's Guide
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Gilson,Inc.
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respective holders. Use of the trademark(s) in this document does not imply any
aliation with or endorsements by the trademark holder(s).
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USER’S GUIDE | ASPEC™ POSITIVE PRESSURE MANIFOLD
CONTENTS
CONTENTS
Safety | 6
Symbols, Signs, and Notices | 6
Chemical Hazards | 7
Flammable Solvents | 7
Replacement Parts | 7
Signs of Damage | 7
Site Requirements | 7
Storage and Movement | 7
Sécurité | 8
Symboles et Notes | 8
Risques Chimiques | 9
Solvants Inflammables | 9
Pièces de Remplacement | 9
Signes de Détérioration | 9
Exigences du Site | 9
Stockage et Déplacement | 9
Introduction | 10
Description | 10
Technical Specifications | 19
Setup | 20
Set up or Replace the Manifold | 20
Connect the Gas Supply | 21
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ASPEC® POSITIVE PRESSURE MANIFOLD | USER'S GUIDE
CONTENTS
Operation | 22
Setup for Flow to Waste | 22
Setup for Flow to Collection | 23
Seat the Manifold | 24
Unseat the Manifold | 24
Control the Flow | 25
General Flow Guidelines | 27
Basic SPE Technique | 28
Maintenance | 31
Clean the ASPEC PPM | 31
Install or Replace the O-Ring | 31
Replace the Manifold Gasket | 31
Maintenance Parts | 31
Troubleshooting Table | 32
Repair and Return Policies | 36
Before Calling Us | 36
Warranty Repair | 36
Non-Warranty Repair | 36
Return Procedure | 37
Unit End-of-Life | 37
Replacement Parts and Accessories | 38
Configuration Kits | 39
ASPEC Positive Pressure Unit | 39
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USER’S GUIDE | ASPEC™ POSITIVE PRESSURE MANIFOLD
SAFETY
Safety
Read this section before installing and operating the instrument.
Only trained technical personnel in a laboratory environment may use the
instrument for non-medical, liquid handling purposes. For safe and correct use
of the instrument, operating and service personnel must follow all instructions
contained in this guide when installing, cleaning, and maintaining the instrument.
All safety precautions must be observed during all phases of operation, service,
and repair of the instrument.
Failure to comply with these precautions or with warnings described in the user’s
guide violates safety standards of design, manufacture, and intended use of the
instrument. Gilson assumes no liability for customers failing to comply with these
requirements.
Refer to the rear panel label on the instrument for the current standards to which
the instrument has been found compliant.
Read all documentation and safety information for accessories, peripherals, and
other instruments that may be used with this instrument before operating the
system.
Symbols, Signs, and Notices
Safety Notices
The following safety notices may appear in this document:
WARNING
Indicates a potentially hazardous situation which, if not
avoided, may result in personal injury.
CAUTION
Indicates a potentially hazardous situation which, if not
avoided, may result in minor or moderate injury.
NOTICE
Indicates a potentially hazardous situation which, if not
avoided, may result in equipment damage.
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ASPEC® POSITIVE PRESSURE MANIFOLD | USER'S GUIDE
SAFETY
Hazard Symbols
The following hazard symbol may appear on the instrument or in this document:
Symbol Explanation Explication Erläuterung
Caution Attention Vorsicht
Chemical Hazards
Any chemicals used for analysis should be handled according to good laboratory
practice (GLP). They should also be stored, used, and disposed of in accordance
with the manufacturer’s specifications, as well as local and national regulations.
Potentially hazardous chemicals can be used with the instrument. Use care when
handling chemicals and wear appropriate personal protective equipment (PPE),
such as safety glasses, gloves, etc.
The responsible individual must ensure that personnel are not exposed to
hazardous levels of toxic substances as outlined in the material safety data sheets
(MSDSs), or any documentation provided by local governing bodies such as The
Health Protection Agency (United Kingdom) or The Occupational Safety and
Health Administration (United States).
Flammable Solvents
Secure all flammable solvents. The temperature of liquids inside the system must
be 25°C (77°F) below the lowest flame temperature of any solvents used.
Replacement Parts
Be sure to use only replacement parts mentioned in this user’s guide. Refer to
Replacement Parts and Accessories on page 38.
Signs of Damage
Do not attempt to use the instrument if there are visible signs of damage.
Site Requirements
Do not operate the instrument if site conditions are not within specifications.
Refer to Technical Specifications on page 19 for more details.
Storage and Movement
Empty the drain well before moving the instrument.
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USER’S GUIDE | ASPEC™ POSITIVE PRESSURE MANIFOLD
SÉCURITÉ
Sécurité
Veuillez lire attentivement ce paragraphe avant toute installation ou utilisation de
l’instrument.
Cet instrument est exclusivement destiné à être utilisé dans un environnement de
laboratoire, par un personnel qualifié, à des fins de manipulations de liquides nonmédicales. Pour une utilisation correcte et en toute sécurité de l’instrument, il est
nécessaire que le personnel qui utilise et réalise la maintenance de l’instrument,
suive les instructions contenues dans ce guide lors de l’installation, du nettoyage
et de la maintenance de l’instrument. Toutes les consignes de sécurité doivent
être respectées durant toutes les phases de fonctionnement, d’entretien ou de
réparation de l’instrument.
Le non-respect de ces précautions ou des avertissements spécifiques mentionnés
dans ce guide compromet les normes de sécurité de conception, de fabrication
et d’utilisation prévue de l’instrument. Gilson décline toute responsabilité en cas
d’incapacité du client à se conformer à ces exigences.
Merci de vous reporter aux indications mentionnées sur le panneau arrière de
l’instrument pour connaître les normes auxquelles l’instrument a été déclaré
conforme.
Veuillez lire toute la documentation et les informations relatives à la sécurité pour
les accessoires, périphériques, et autres instruments pouvant être utilisés avec cet
instrument avant de faire fonctionner le système.
Symboles et Notes
Notes de Sécurité
Les notes de sécurité suivantes peuvent apparaître dans ce document:
AVERTISSEMENT
Une situation potentiellement dangereuse qui, si
elle n’est pas évitée, peut entraîner des dommages
corporels.
AT TENTION
Une situation potentiellement dangereuse qui, si
elle n’est pas évitée, peut entraîner des blessures
mineures ou légères.
AVIS
Une situation potentiellement dangereuse qui, si
elle n’est pas évitée, peut entraîner des dommages
matériels.
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ASPEC® POSITIVE PRESSURE MANIFOLD | USER'S GUIDE
SÉCURITÉ
Symbole de Dangers
Le symbole de dangers suivants peuvent apparaître sur l’instrument ou dans ce
document:
Symbol Explanation Explication Erläuterung
Caution Attention Vorsicht
Risques Chimiques
Tous les produits chimiques utilisés pour l’analyse doivent être manipulés
conformément aux bonnes pratiques de laboratoire (BPL). Ils doivent être
stockés, utilisés et éliminés conformément aux recommandations du fabricant
ainsi qu’aux réglementations locales et nationales. Manipuler les produits
chimiques avec précaution et porter un équipement de protection individuelle
adapté (EPI) comme des lunettes de sécurité, des gants, etc.
Le responsable doit veiller à ce que le personnel ne soit pas exposé à des niveaux
dangereux de substances toxiques comme indiqué dans les fiches de données
de sécurité (fDS), ou toute documentation fournie par les instances locales telles
que l’agence de protection de la santé (Royaume-Uni) ou l’administration de la
sécurité et de la Santé (Etats-Unis).
Solvants Inflammables
Prendre garde aux solvants inflammables. La température des solvants
à l’intérieur du système doit impérativement être inférieure de 25°C à la
température de flamme la plus basse des solvants utilisés.
Pièces de Remplacement
Utiliser exclusivement les pièces détachées mentionnées dans ce guide utilisateur.
Faire référence aux Replacement Parts and Accessories on page 38.
Signes de Détérioration
Ne pas utiliser le système en cas de signes de détérioration visibles.
Exigences du Site
Ne pas faire fonctionner l’instrument si le site présente des conditions non
conformes aux spécifications. Faire référence aux Technical Specifications on
page 19.
Stockage et Déplacement
Vider le conteneur d’evacuation des solvants usages avant de deplacer l’appareil.
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USER’S GUIDE | ASPEC™ POSITIVE PRESSURE MANIFOLD
INTRODUCTION
Introduction
Description
The ASPEC® Positive Pressure Manifold provides uniform and consistent
flow rate control for the cleanup of low or high viscosity samples, improves
solid-phase extraction (SPE) reproducibility and recovery. The ASPEC®PPM
incorporates easily into laboratory workflows and provides a fast and simple
way to prepare several samples for later analysis by chromatography.
The ASPEC manifold offers a cost-effective approach to automation for
environmental, clinical, forensic, and food or beverage testing laboratories. It
is also an ideal choice for SPE cleanup prior to LCMS for bioanalyses.
Drain Well
Refer to page 12 for
a detailed description.
SPE Racks
15-, 24-, and
96-positions available.
Figure 1
Standard Components of the ASPEC™ Positive Pressure Manifold (Left View)
SPE Collection Racks
15-, 24-, and 96-positions available.
Pressure
Gauge
Monitors the
operating
pressure of the
system, which
should not
exceed 15 psi.
SPE Control Dial
Switch between Low Flow and High Flow when
the process control dial is set to SPE. Refer to
page 12 for a detailed description.
Process Control Dial
Switch between SPE and
Dry controls to adjust gas
flow. Refer to page 12
for more information.
Manifold
Locking Lever
Forms the seal
between the
manifold and SPE
cartridge when
locked. Refer to
page 14.
Safety Shield
Protects users
from solvents.
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ASPEC® POSITIVE PRESSURE MANIFOLD | USER'S GUIDE
INTRODUCTION
Figure 2
Standard Components of the ASPEC™ Positive Pressure Manifold without Shield
Flow Adjustment Locks
Used to lock in flow
adjustments made with the
low and high flow regulator
knobs. Refer to page 13.
High Flow
Adjustment Knob
Used to fine tune flow
when High Flow is
selected on the SPE
control dial. Refer to
page 13.
Low Flow
Adjustment Knob
Used to fine tune
flow when Low Flow
is selected on the
SPE control dial.
Refer to page 13.
Manifold
Positioning Lever
Raises and lowers the
manifold over the SPE
cartridge holder. The
manifold locking level
must be unlocked to
move this lever.
Refer to page 14.
Locator Plate
Refer to page 14 for a
detailed description.
Thumbscrews
Manifold
Retaining Clip
Holds the manifold
in place. Refer to
page 20.
Manifold
Available in three
configurations: 15- or
24-cartridge holders and
96-well plate holders.
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USER’S GUIDE | ASPEC™ POSITIVE PRESSURE MANIFOLD
INTRODUCTION
Control Dials
Process Control Dial
Used to select SPE (activates
the SPE control dial to control
high or low flow) or Dry
(maximum flow).
SPE Control Dial
Used to select Low Flow,
High Flow, or Off (to stop flow)
when the process control dial is
set to SPE.
Drain Well
Used primarily to
flow the preparation
solvents and matrix
to waste. Ensure
that the waste
outlet tubing flows
to a large waste
container.
Ejection Plate
Used to quickly eject all the SPE
cartridges from the cartridge rack.
Slide the ejector plate through the
standoffs on the SPE cartridge
rack, and then push the plate up
to eject the SPE cartridges.
Figure 3
Process Control Dial
Figure 4
SPE Control Dial
Figure 5
Collection and Drain Well with Waste Outlet Tubing
Front
Rear
Waste
Outlet
Tubing
Barbed
Fitting
Standos
SPE Cartridges
Ejector
Plate
Figure 6
Ejecting SPE Cartridges
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