Gilson 402 User Manual
402 Dilutor Dispenser
User’s Guide
402 Dilutor Dispenser
User’s Guide
LT801310/C - ©2003 Gilson SAS All rights reserved November 2003
Contents
Table of Contents 0
Safety
1 Introduction
Notational Conventions .................................................................. 1-2
Overview .......................................................................................... 1-3
Unpacking ...................................................................................... 1-5
Warranty ......................................................................................... 1-6
Customer Service .......................................................................... 1-7
2 Description
Single-Syringe Model ....................................................................... 2-2
Front Panel ................................................................................ 2-2
Rear Panel ................................................................................. 2-2
Dual-Syringe Model ...................................................................... 2-3
Front Panel ................................................................................ 2-3
Rear Panel ................................................................................. 2-3
Keypad and Probe ......................................................................... 2-4
Keypad ...................................................................................... 2-4
Probe .......................................................................................... 2-4
Hydraulic Components ............................................................... 2-5
Valve .......................................................................................... 2-5
Tee-junction .............................................................................. 2-5
Syringes ..................................................................................... 2-5
3 Installation
Assembling the Probe ...................................................................... 3-2
For Standard Use ........................................................................ 3-2
Adapter Kit for Disposable Tips ................................................ 3-2
Connecting the Tubing .................................................................... 3-4
Single-Syringe Model ................................................................. 3-4
Dual-Syringe Model ................................................................ 3-5
Electrical Connections.................................................................. 3-7
Sector Block ............................................................................... 3-7
Voltage Selector........................................................................ 3-7
Installing the Fuses ................................................................. 3-7
Connecting the Power Cord .................................................. 3-8
Connecting the Keypad.......................................................... 3-8
Connecting the Probe .............................................................. 3-8
Syringes........................................................................................... 3-9
Assembling a Syringe ............................................................. 3-9
Fitting a Syringe ....................................................................... 3-9
Table of Contents-1
Upgrading to Dual-Syringe Model ........................................... 3-10
Removing the Cover .............................................................3-11
Connecting the Flat Cable.................................................... 3-11
Inserting the Module ............................................................ 3-11
4 Operation - Standard Use
Essential Steps .................................................................................. 4-2
Standard Parts.......................................................................... 4-2
Connect the Keypad ................................................................ 4-2
Fit the Fuses and Connect the Power ................................... 4-2
Assemble and Connect the Probe ......................................... 4-2
Connect the Tubing ................................................................. 4-2
Switch-on .................................................................................. 4-3
Configuration (Conf) .............................................................. 4-4
Fit the Syringe(s) ...................................................................... 4-7
Creating a Method (Edit) ....................................................... 4-7
Prime the 402 (Man) .............................................................. 4-10
Running a Method (Run) ..................................................... 4-11
5 Operation - Advanced Use
Correct Use of the 402 ................................................................... 5-2
Single-Syringe Model .............................................................. 5-2
Dual-Syringe Model ................................................................ 5-2
Software .......................................................................................... 5-5
Entering the Configuration (Conf)........................................ 5-6
Creating a Method (Edit) ....................................................... 5-9
Saving a Method.................................................................... 5-10
File Manipulations (File) ...................................................... 5-10
Direct Manipulations (Man) ............................................... 5-12
Tasks - Detailed Descriptions .............................................. 5-15
Additional Information ............................................................. 5-31
Flow Rates............................................................................... 5-31
Syringe Correction Function................................................ 5-33
Use of Disposable Tips .........................................................5-33
6 Maintenance
Table of Contents-2
Cleaning ......................................................................................... 6-2
Cleaning the Exterior .............................................................. 6-2
Cleaning the Valve.................................................................. 6-2
Cleaning the Tee Junction ...................................................... 6-3
Cleaning the Syringe Assembly ............................................ 6-4
Autoclaving.................................................................................... 6-5
Autoclaving the Tubing ......................................................... 6-5
Changing the Syringe Piston Assembly.................................... 6-6
Replacing the Piston ............................................................... 6-6
7 Troubleshooting
Appendices
Changing the Piston Syringes and Piston Noses .................... 6-7
Changing the 100 and 250 µL Piston Noses ...................... 6-7
Changing the 500 µL Piston Noses ......................................6-9
Changing the 1 mL Piston Noses ....................................... 6-10
Changing the 25 mL Piston Noses ..................................... 6-11
Changing the 5 mL and 10 mL Piston Noses................... 6-11
Mechanical Problems ................................................................... 7-2
Hydraulic Problems ...................................................................... 7-3
Electrical Problems ........................................................................ 7-4
A - Parts and Accessories Lists ....................................... Appendix A-1
402 Single Syringe ............................................... Appendix A-2
402 Dual Syringe with Tee Junction Config. ...Appendix A-3
Additional Accessories....................................... Appendix A-4
Spare Parts ............................................................ Appendix A-5
B - Chemical Compatibility ..................................... Appendix B-2
Materials in Contact with Liquids ................... Appendix B-2
Compatibility ....................................................... Appendix B-2
C - Technical Data ..................................................... Appendix C-1
Hydraulics ............................................................Appendix C-2
Mechanics............................................................. Appendix C-5
Environmental .....................................................Appendix C-6
D - Error Messages ................................................... Appendix D-2
Index
Table of Contents-3
Safety 0
Safety
Read this section carefully before installing and operating the instrument.
For safe and correct use of the instrument, it is essential that both operating and
service personnel follow generally accepted safety procedures as well as the safety
instructions given in this document, the 402 Dilutor-Dispenser User’s Guide.
The instrument described in this document is a syringe operated pump for transferring liquids. It should only be used in the laboratory or similar indoor environment for
analytical purposes, by qualified personnel. If an instrument is used in a manner not
specified by Gilson, the protection provided by the instrument may be impaired.
Voltages present inside the instrument are potentially dangerous. If there is a problem
with the instrument, the power cable should be removed until qualified service personnel
have repaired it. This is to prevent anyone from inadvertently using the instrument,
thus causing possible harm to themselves, or damage to the instrument itself.
The leakage current of this instrument is within the limits allowed by international
safety standards for laboratory equipment. An efficient ground connection is imperative for the physical protection of the user.
Power supply cord reference 7080316106 is for use in France and Germany. Power
supply cord reference 7080316105 is for use in USA and Canada. For other countries
contact your local Gilson distributor. You must only use the type of fuse described in
this document: 1.0 Amp type “T” slow blow.
Adequate protection including clothing and ventilation must be provided if dangerous
liquids are used in the analytical work. In case of incidental spillage, carefully wipe with a
dry cloth, taking into account the nature of the spilled liquid and the necessary safety
precautions.
Cleaning, dismantling, maintenance, and repair should only be performed by personnel
trained in such work, and who are aware of the possible dangers involved.
Safety-1
Safety
!
1
Individual components:
parts of the valve, the Tee
junction, and the syringes
(except for the 25 mL syringe)
may be autoclaved after
dismounting (121°C, 0.1 MPa,
20 min).
Symbol Explanation
Alternating current
~
PROTECTIVE CONDUCTOR
TERMINAL
On (Supply switch)
I
Off (Supply switch)
O
This instrument1 must not be sterilized, using an
autoclave, or any other mechanical device. When
you need to clean an instrument, use one of the
three following methods:
1 - a clean dry cloth,
2 - a cloth dampened with water,
3 - a cloth dampened with soapy water.
If a cloth dampened with soapy water is used to clean
the instrument, only domestic soap may be used.
No other form of detergent or chemical may be
used.
These electronic and hazard symbols
appear on the 402 Dilutor Dispenser:
Caution, risk of electric shock
Caution (refer to User’s Guide)
Safety-2
Introduction 1
The following chapter describes the instrument, the service and warranty conditions.
1-1
Introduction 1
Notational Conventions
The conventions in this User’s Guide are as follows.
Used in the stand-alone mode, this instrument is
referred to as ‘402 Dilutor-Dispenser’ or simply 402.
Specific keys to press on the Gilson Keypad are
represented in bold capitals, for example: ‘key in
the value and press ENTER’; where ENTER is a
Notational Conventions
key on the keypad.
For a soft-key that must be pressed, the function of
that key is represented in bold, for example: ‘Press
Prime to prime the pump’.
The Gilson Keypad displays on-screen messages as
normal typeface inside a frame.
1-2
Introduction 1
Overview
The 402 Dilutor-Dispenser, is used to transfer liquids
between vials according to user defined protocols
(Methods). You program the 402 Dilutor-Dispenser
via the keypad, which includes an LCD screen, for
communication purposes.
You may store a total of 99 linkable Methods using
the keypad. Each Method consists of one or more of
the following tasks: Dilute, Dispense, Mix, Pipette,
and Rinse. In addition three functions for direct
manipulation are available: Priming, Titration,
Volume Measurement.
Gilson’s 402 Dilutor-Dispenser is available in two
configurations: Single-syringe, or Dual-syringe.
The 402 Dilutor-Dispenser is very versatile; it may
be fitted with seven sizes of syringe. The standard
402 Dilutor-Dispenser is equipped with a handheld probe for aspirating and dispensing liquids.
Optionally, the probe may be fitted with disposable
tips (Gilson Diamond® Tips, D200 and D1000).
Overview
This versatility means that your 402 Dilutor-Dispenser
may be used for a wide variety of applications
including the chemical field where the ability to
handle large volumes or high dilution ratios may be
appreciated, and the biological field where small
volumes and protection against cross-contamination
are important (see Autoclavability, page 6-5).
It can even be used with corrosive liquids (see
Chemical Compatibility, Appendix B).
Refer to Appendix A for lists of the standard
equipment, additional accessories and spare parts.
The 402 DD is supplied with a tubing kit for connecting to a solvent reservoir. Syringes are not
included in the standard equipment; you should
order syringe(s) appropriate to your Method (volume,
systematic error and flow rate requirements; refer to
Appendix C). Also, a Tee junction kit (not including the syringe) is available for upgrading a Singlesyringe Dilutor-Dispenser to the Dual-syringe version.
1-3
Introduction 1
Good Laboratory Practice (GLP) is facilitated by the
following:
Overview
씮 codes to protect the program files against unau-
thorized modification,
씮 counters for the number of syringe and valve
operating-cycles,
씮 checking protocol for the hydraulic specifica-
tions (IQ/OQ procedures),
씮 correction of the syringe-piston stroke to com-
pensate for environmental factors (temperature,
viscosity, etc.).
1-4
Introduction 1
Unpacking
Your 402 Dilutor-Dispenser is shipped in a box
together with its various accessories. The original
box and packing material should be kept in case
the equipment has to be returned to your Gilson
distributor.
You should check off the parts received in the box
against the parts list (refer to Appendix A), at the
time that you receive the shipment, even if the 402
is not to be used immediately. Your Gilson distributor should be notified immediately of any inconsistencies between the contents of the box and the
parts list, or if any of the parts appear to have been
damaged in transit.
Unpacking
1-5
Introduction 1
Warranty
Warranty
If the 402 Dilutor-Dispenser does not appear to
function correctly, first verify the electrical connections are correct and that the instrument is
switched ON. Contact your Gilson distributor for
technical advice or possibly a service visit. Any
service required will be given within the warranty
conditions assured by your Gilson distributor.
1-6
Introduction 1
Customer Service
Gilson and its worldwide network of authorized
distributors provide you with four types of assistance: sales, technical, applications and service.
Customer Service personnel are able to serve you more
efficiently if you provide the following information:
• The serial number and model number of the
equipment involved.
• The installation procedure you used.
• A concise list of the symptoms.
• A list of operating procedures and conditions you
were using when the problem arose.
• A list of other devices connected to the system (if
any) and a system diagram showing the connections.
Customer Service
• A list of other electrical connections in the room.
1-7
Introduction 1
1-8
Description 2
The following chapter describes the mechanics and hydraulics of the Dilutor-Dispenser.
Two models of 402 Dilutor-Dispenser are available: Single-syringe and Dual-syringe.
Each model comes with a keypad, a probe, and accessories. You order and fit the
syringe(s), from those described on page 2-6, most suited to your application.
Your 402 is delivered with the appropriate modules fitted. If later, you decide to
convert a Single-syringe model into a Dual-syringe model, you must order and fit a
Tee-junction module to the right-hand side of your dilutor; the left-hand always
contains a Valve module.
2-1
Description 2
Single-syringe Model
The Single-syringe model has a Valve module at the
left, and a blanking plate at the right.
The Valve module includes one motor to operate
the syringe and another motor to switch the valve.
It is a liquid transfer station that can be used to
Single-syringe Model
deliver up to 25 mL in one stroke, and down to 1 µL
(typical value), with high accuracy.
Bottle Clip for Tubing
Valve Module
ON/OFF indicator
Tubing Clip (Self-adhesive)
Cable Support
Clamp for Probe
2-2
Microswitches
ON/OFF Switch
Front Panel
The front panel holds the hydraulic components
and is fitted with an ON/OFF indicator.
Rear Panel
The rear panel holds a socket for connecting the
keypad, a connector for the probe, the ON/OFF
switch, and voltage selector/fuse holder. There is
also a Gilson Serial Input Output Channel (GSIOC)
connector, which is a communications channel to
link the 402 Dilutor-Dispenser with other equipment,
if required. There is also a bank of microswitches
for use in setting up the communications channel.
Description 2
Dual-syringe Model
The Dual-syringe model is fitted with a Valve
module at the left and a Tee-junction module at the
right. The modules are linked by plastic tubing.
Dual-syringe Model
Bottle Clip for Tubing
Valve Module
ON/OFF indicator
Front Panel
Tubing Clip (Self-adhesive)
Cable Support
Clamp for Probe
The front panel holds the hydraulic components
and is fitted with an ON/OFF indicator.
The Valve module includes one motor to operate the
syringe and another motor to switch the valve. The
Tee-junction module has only one motor, to operate
the syringe.
The Dual-syringe model may be fitted with syringes
of different volumes. The left syringe is the large
volume syringe and is used to deliver a large volume
of liquid in a minimum time. The right syringe can
be used to transfer smaller volumes with high
reproducibility and accuracy (refer to Appendix C
for Technical Data). The benefits of this configuration
are a large dilution ratio with the best reproducibility and fast transfer times.
Rear Panel
This is the same for both models.
2-3
Description 2
Keypad and Probe
Keypad
The keypad includes an LCD display (two 24character lines), membrane touch-keys, and a
connecting cord.
Keypad and Probe
Numeric keys: the keypad has the numbers 0 to 9, a
decimal point key, and the Enter key.
Five Soft-keys: the function of each key depends on
the screen that is displayed (e.g. Next,
Prev, Chge...).
STOP key: Press this key to stop the current ac-
tion (Method or direct manipulation).
HELP key: Press this key to access help messages
relating to the current screen.
ESC key: Press this to quit a menu or return
to the first menu of the software
branch.
CLEAR key: Press this key to cancel a value and
return to the previous value before
validating it.
Probe
The assembled probe fitted with the standard probe
nozzle is shown opposite. Assembly and installation
of the standard probe is described in Chapter 3.
A kit is available to enable you to fit Gilson’s
Diamond® tips (D200, D1000) to the probe (see
Chapter 3).
2-4
Description 2
Hydraulic Components
Valve
Hydraulic Components
The two-position valve, which is fitted to the lefthand side of the 402, has three ports. It switches
the syringe port to either the reservoir or to the
probe side (see opposite figure).
The valve, which has a 0.5 sec rotation period, is
driven by a stepper motor. It is designed to withstand a million rotations, when used with water.
The liquid contact surfaces of the valve, which is
autoclavable, are in PEEK and ceramic (121°C,
0.1 MPa, 20 min).
Tee Junction
The Tee-junction, which is fitted to the right-hand
side of the dual-syringe model, has three ports: the
inlet from the valve, the outlet to the probe, and the
syringe port (see opposite figure).
The Tee-junction, which has no internal moving
parts, includes 2 channels, each 1.5 mm in diameter oriented at 120°. The liquid contact surfaces of
the Tee-junction, which is autoclavable, are in PEEK.
From reservoir
To waste
From Valve
Probe (or Tee
junction)
Syringe
To Probe
Syringe
Syringes
The choice of syringe allows the 402 Dilutor-Dispenser
to handle volumes from 1 µL to 25 mL with low
values of systematic and random error. The 402
Dilutor-Dispenser is able to transfer liquid as
follows:
- from 1 µL to 10 mL from the probe (transfer
tubing volume of up to 10 mL),
- up to 25 mL from the reservoir, in one stroke,
- up to 99 mL from the reservoir, in several strokes.
When using the Dual-syringe model, a theoretical
dilution ratio of 1/25000 is achieved in one stroke
of both sample and diluent syringes. However, you
are advised not exceed a ratio of 1/500 for any
given dilution, in order to stay within the RSD
range (after efficient mixing).
2-5
Description 2
0.1 mL
0.25 mL
0.5 mL
Hydraulic Components
5 mL
10 mL
1 mL
25 mL
There are seven syringe sizes available for the 402,
all except the 25 mL are autoclavable. The lifetime
of all pistons (see Chapter 6), when they are used
with water at a pressure of up to 0.3 MPa is over
30000 cycles (each cycle contains 2 strokes: one up
and one down).
The available syringe sizes are:
Part Number Description
25025341 0.1 mL
25025342 0.25 mL
25025347 0.5 mL
25025343 1 mL
25025344 5 mL
25025345 10 mL
25025346 25 mL
Syringe Materials
Part of the syringe Material
Syringe body Borosilicate glass
Syringe adapter 316L stainless steel
Piston Stainless steel (models up to 1 mL)
Aluminum
Piston nose PTFE/Ekonol (PTFE for the 25 mL)
2-6
Installation 3
This chapter explains how to install the fuses, syringes and hydraulics according to
your system requirements.
This chapter explains how to add the extra module to upgrade your 402 to a dual
syringe pump.
3-1
Installation 3
Assembling the Probe
For Standard Use
Access Point
Probe
Assembling the Probe
Probe
Nozzle
Transfer Tubing
When you want to increase the
length of tubing protruding
from the probe, you must first
carefully lift the tubing out of
the handle, otherwise you will
damage the tubing.
In standard use the probe is used with the 2 mL
tapered transfer-tubing (see opposite figure).
1. Thread the tapered end of the transfer-tubing
through the plastic ‘tunnels’ in each of the 5 premounted cable supports (see figure below).
2. Lay the tubing on the probe’s groove such that
about 4 cm of the tapered end protrudes from
the end of the probe.
3. Push the tubing into the groove in the probe at
the 3 round access points using (for example)
the rounded end of a pencil. Do not press on the
tubing with your fingernail.
4. Push the tapered end of the tubing through the
standard tip, then screw the tip onto the end of
the probe.
5. Plug the end of the control cable into the socket,
labeled ‘PROBE’ at the back of the 402.
6. Fit the clamp for probe at the right-hand side of
the 402 (see opposite figure).
7. Mount the probe on the clamp.
8. Connect the other end of the tubing to the outlet
of the Valve (Single-syringe) or the Tee-junction
(Dual-syringe).
9. Use the 402 Software to check (and change, if
necessary) the configuration using the Conf
(Vol) menu.
Adapter Kit for Disposable Tips
The ability to use Diamond Tips will extend the use
of your 402 into the biological field, where protection against cross-contamination is important, and
in the chemical field to protect the tubing from
corrosive liquids.
You must fit a tip-holder to the probe when you
want to use Gilson’s Diamond tips. An adapter kit
(reference F4015015) is available to permit you to
fit them to the probe. The kit includes two sizes of
tip-holder (with built-in tip ejector), and a special
non-tapered 2 mL transfer tubing, as follows.
3-2
Installation 3
2 Special adapters for the probe
1 D200 tips, pack of 10
1 D1000 tips, pack of 10
1 Transfer-tubing (outlet) non-tapered, FEP (2 mL)
1. Lay the tubing on the probe’s groove such that
the end with the small washer is just at the
orifice of the probe.
2. Push the tubing into the groove in the probe at
the 3 round access points using the rounded end
of a pencil (for example). Do not press on the
tubing with your fingernail.
3. Screw the tip-holder onto the end of the probe.
4. Plug the end of the control cable into the socket,
labeled ‘PROBE’ at the back of the 402.
5. Fit the clamp at the right-hand side of the 402
(see on the previous page).
6. Mount the probe on the clamp.
7. Connect the other end of the tubing to the outlet
of the valve (Single-syringe) or the Tee junction
(Dual-syringe).
(1) The small washer is important
in that it helps to ensure that
fluid does not leak from the
junction between the tip-holder
and the orifice of the probe.
(2) The special ‘S’ shaped metallic
clips, which are pre-mounted, are
for supporting the tubing on the
black control cable. The tubing
for use with the tip-holder will
not pass through the plastic
‘tunnels’ in the pre-mounted
cable supports. However, to
make the metallic clip easier to
attach, you can push these black
tubing-supports to the end of
the cable.
Assembling the Probe
8. Inform the 402 Software of the new configuration using the Conf (Vol) menu.
9. Fit a tip to the holder. In use, when you change
a tip, wipe the end of the tip-holder first, to
remove any liquid from the orifice. Do not
twist the probe to the left, when fitting a tip,
otherwise the tip-holder will unscrew itself.
3-3
Installation 3
Connecting the T ubing
Tubing connectors should be hand-tightened,
tools should not be used as their use will damage
the connector’s thread.
Single-syringe Model
Connecting the T ubing
From
reservoir
Transfer tubing
to probe
Valve Inlet Tubing
A length of Fluoride Ethylene Propylene (FEP)
tubing, supplied with the 402, is used to connect
the inlet port to the solvent reservoir. Screw the
connector into the inlet port (left-hand) of the valve
and put the other end into the reservoir. A bottle
clip is provided to hold the tubing securely in the
reservoir (see opposite Figure).
An alternative tubing, fitted with a connector at
one end and a 20 µm filter at the other, is available
as an accessory (reference 3645357).
Waste Tubing
Connect a suitable length of tubing from the waste
outlet of the valve to a suitable receptacle. The valve
releases liquid through the waste outlet if the pressure
inside the valve is too high. For the pressure limits
of the valve, refer to Appendix C, Technical Data.
Transfer Tubing (Probe)
3-4
To waste
V alve
This tubing (FEP) allows the transfer of samples
aspirated from the probe and prevents the contamination of the syringe(s). The size of transfer tubing
is chosen as a function of the volumes of samples,
reagents, and air gaps that have to be handled via
the probe. The total volume transferred must be less
than the volume of the transfer tubing.
The transfer tubing connects the outlet port (righthand) of the valve to the probe. A 2 mL tapered
tubing is supplied as standard; 1 mL, 5 mL, and
10 mL are available as additional accessories.
Self-adhesive clips are provided for holding the
transfer tubing securely against the side of the 402.
The 1 mL transfer tubing is recommended for transferring volumes smaller than 1 mL. To avoid movements of the tubing that could affect the precision,
the 5 mL and 10 mL transfer tubings are rolled and
held by magnets on the left-side of the 402.
Installation 3
After assembling the probe, the other end of the
tubing is installed by screwing the connector into
the right-hand port of the valve. When you use
Gilson Diamond Tips, the special non-tapered
tubing, included in the kit, must be fitted.
Dual-syringe Model
Valve Inlet Tubing
A length of Fluoride Ethylene Propylene (FEP) tubing, supplied with the
402, is used to connect the inlet port
to the solvent reservoir. Screw the
connector into the inlet port (lefthand) of the valve and put the other
end into the reservoir. A bottle clip is
provided to hold the tubing securely
in the reservoir.
An alternative tubing, fitted with a
connector at one end and a 20 µm
filter at the other, is available as an
accessory (reference 3645357).
From
reservoir
To waste
Connection tubing
Transfer tubing
to probe
Left
Syringe
V alve Tee-junction
Right
Syringe
Connecting the T ubing
Valve Waste Tubing
Connect the length of tubing (supplied as standard)
to the waste outlet of the valve, and put the other
end into the waste or any other suitable receptacle.
The valve releases liquid through the waste outlet,
if the pressure inside the valve is too high (see
Appendix C, Technical Data).
Connection Tubing (Valve to Tee-junction)
This tubing has connectors at both ends and is used
to connect from the valve outlet port (right-hand) to
the Tee-junction inlet port (left-hand). It is made of
FEP and has the following dimensions: 70 x 3 x 2 mm.
It is autoclavable (20 min. at 0.1 MPa, 121 °C).
Transfer Tubing (Probe)
This tubing (FEP) allows the transfer of samples
aspirated from the probe and prevents the contamination of the syringe(s). The size of transfer tubing
is chosen as a function of the volumes of samples,
reagents, and air gaps that have to be handled via
the probe. The total volume transferred must be
less than the volume of the transfer tubing.
3-5
Installation 3
The transfer tubing connects the outlet port (righthand) of the Tee-junction to the probe. A 2 mL
tapered transfer-tubing is supplied as standard ;
1 mL, 5 mL, and 10 mL are available as additional
accessories.
Self-adhesive clips are provided for holding the
transfer tubing securely against the side of the 402
(see previous page).
Connecting the T ubing
The 1 mL transfer tubing is recommended for
transferring volumes smaller than 1 mL. To avoid
movements of the tubing that could affect the
precision, the 5 mL and 10 mL transfer tubings are
rolled and held by magnets on the left-side of the
402.
After assembling the probe, the other end of the
tubing is installed by screwing the connector into
the right-hand port of the Tee-junction. When you
use Diamond tips, the special non-tapered tubing,
included in the kit, must be fitted.
3-6
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