GE P&W Sievers Nitric Oxide Analyzer User Manual

GE

Water & Process Technologies
Analytical Instruments

Sievers

Nitric Oxide Analyzer (NOA 280i)

Overview

The Sievers Nitric Oxide Analyzer (NOA*280i) offers the
most versatile detection system for Nitric Oxide (NO)
analysis. Employing GE Analytical Instruments’ highly
sensitive, ozone-chemiluminescence technology, the
NOA 280i has unsurpassed versatility for liquid and
exhaled breath NO measurement. With over 1,000
publications using the Sievers Nitric Oxide Analyzer, the
NOA has proven to be the instrumentation of choice by
researchers worldwide.

Applications

Liquid Samples

The NOA 280i measures nitric oxide, nitrite, nitrate or
nitrosothiols in most biological samples. Concentrations,
ranging from low nanomolar to millimolar levels, can be
measured in sample volumes ranging from a few
microliters to several milliliters.

Exhaled Breath Samples

Exhaled nitric oxide may be a useful marker of airway
inflammation and can easily be measured with the
NOA 280i. Fast response time and low sample flow
rates allow for measurement of exhaled NO in humans
and animals.

Fact Sheet

Low-Cost Maintenance

Requires minimal maintenance—consumables are
inexpensive and require replacement approximately
every six months (900 hours of operation). The firmware
tracks operating time and alerts the user when to
replace consumables or perform routine maintenance.

Compact Size

Compact footprint conserves valuable bench space.
The vacuum pump can be conveniently located either
under the bench or on a cart with the analyzer.

Powerful Data Collection and Analysis

NOAnalysis,*custom-designed software for data
collection and analysis, includes: Liquid, Restricted
Exhaled Breath, Breath-by-Breath and Bag Sampling
Programs. With four different programs for liquid and
gas-phase measurements, the user can process data
over the wide range of sampling techniques.

Features

User-Friendly Operation

Easy-to-use, menu-based firmware provides complete
analyzer control. Create, store and easily upload meth­ods for quick retrieval of multiple application parameters.

Full-Range Service Support

GE Analytical Instruments offers a full range of factory
and on-site services.

Easy-to-Perform Calibration

Calibration is easy-to-perform with the GE Analytical
Instruments Calibration Kit.

Liquid Applications

Exhaled Breath Applications

Measure nitric oxide and its reaction products, nitrate,
nitrite and nitrosothiols, in virtually any biological fluid
including: plasma, sera, urine, cerebral-spinal fluid, cell
culture media, tissue homogenates and perfusates.

Nitrate

Nitrate (NO
some cell culture systems and most physiological
fluids, is formed when nitric oxide reacts with
oxyhemoglobin or superoxide anion. Use the NOA 280i
to measure total nitric oxide by converting the nitrate
and nitrite to nitric oxide using vanadium (III) chloride in
hydrochloric acid at 90° C.

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), the major oxidation product of NO in

3

Nitrite

Nitrite (NO
absence of oxyhemoglobin or superoxide anion, is
formed when nitric oxide reacts with dissolved oxygen.
Use the NOA 280i to measure nitrite by converting the
nitrite to nitric oxide using room temperature iodide
and acetic acid.

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), the major oxidation product of NO in the

2

Nitrosothiols

Nitrosothiols can be measured directly using the NOA
280i. Measure low molecular weight compounds such
as S-nitrosoglutathione using a Cu(I)/Cysteine reagent.
Measure higher molecular weight species (S-nitroso­albumin or hemoglobin) using iodine, iodide and
acetic acid after chemical or chromatographic removal
of nitrite.

Liquid Accessories

Purge Vessel

Use the NOA 280i, in conjunction with the Purge Vessel,
to measure most reaction products of nitric oxide. The
Purge Vessel’s design ensures quantitative conversion
of nitrate, nitrite, and nitrosothiols to nitric oxide,
resulting in sharp, well-defined data peaks. Clean-up is
quick and simple with the built-in drain port.

Perform all current exhaled NO techniques including:
On-line, Off-line, Nasal, Breath-by-Breath and Chamber
Sampling.

On-Line Sampling

Use our patented technique1and the On-Line NO
Breath Kit*to measure on-line concentrations of NO in
exhaled breath. The NOA 280i’s built-in exhaled pressure
biofeedback system and the optional Thermal Mass
Flowmeter facilitate exhalation at constant flow rates.

Off-Line Sampling

Use GE Analytical Instruments patented technique1and
the Bag Collection and Sampling Kit to collect subjects’
exhaled breath for later analysis. The concentration of
NO in the Mylar®collection bags is stable for 8 to 12
hours. Reuse the bags after a simple cleaning procedure.

Nasal Sampling

Increased levels of nasal nitric oxide have been
identified in subjects with sinusitis, rhinitis and asthma.
On-line measurement of nasal nitric oxide may be a
useful marker of inflammation in the nasal passages
and paranasal sinuses. Use a constant transnasal flow
with slow oral exhalation against a resistance to
provide a fast and repeatable measure of nasal NO.

Breath-by-Breath Analysis

Use the Breath-by-Breath technique to measure
exhaled NO in subjects who cannot perform on-line or
off-line exhaled breath maneuvers, i.e., ventilated
subjects, small children and animals. The NOA 280i’s
auxiliary pressure transducer, and the NOAnalysis soft­ware, determine the beginning and end of each exhala­tion and the concentration of NO for each exhalation.

Chamber Sampling

Use the Chamber Sampling method to measure
exhaled NO from spontaneously breathing small
animals, such as mice and rats.

Other Gas Applications

Use the NOA 280i to measure gas-phase NO in the GI
tract during endoscopy. The NOA 280i can also be used
to measure NO directly in the airways during bron­choscopy.

Use the Bag Collection and Sampling Kit to collect off­line exhaled NO at 50 mL/sec, the same conditions the
ATS recommends for on-line sampling. This kit employs
the dead space volume discard technique with subse­quent collection of exhaled breath in 1.5 L Mylar bags.

Exhaled Breath Accessories

On-Line NO Breath Kit

Use the On-Line NO Breath Kit to accommodate flow
rates from 30 to 250 mL/sec, to meet the American
Thoracic Society’s (ATS) and the European Respiratory
Society’s (ERS) recommendations for on-line exhaled
NO measurement.

Thermal Mass Flowmeter

Use the Thermal Mass Flowmeter with the On-Line
NO Breath Kit for direct measurement of exhalation
flow rate.

Off-Line Sampling Kits

The Off-Line Sampling Kits include an inspiratory gas fil­ter to reduce the concentration of NO in inspired air to <5
ppb. Two different bag collection kits are available.

Following ATS recommendations for off-line exhaled
NO, use the ATS Compliant Bag Collection and
Sampling Kit to collect the entire vital capacity at a flow
rate of 350 mL/sec. The ATS Kit uses 12 L Mylar bags.

Calibration Kit

The Calibration Kit includes a Zero Air Filter,
to generate air containing <1 ppb NO from room air, the
calibration gas, and a low flow regulator.

Calibration Gas

A small, lightweight, disposable cylinder of 45 ppm NO
in N2, which provides sufficient NO calibration gas for
three to four months of operation.

Zero Air Filter

The Zero Air Filter removes NO from ambient air
providing a clean (<1 ppb nitric oxide) air source for
calibration.

Bacterial/Viral Filters

The single-subject, disposable Bacterial/Viral Filters,
with >99.9% bacterial and viral filtration efficiency, are
used with the On-Line NO Breath and the Bag
Collection and Sampling Kits

Purge Vessel

On-Line NO Breath Kit

Bag Collection

and Sampling Kits

Specifications

Specifications

Liquid Samples Gas Samples

Sensitivity 1 picomole (1 nM for a 1 mL injection) 0.5 ppb
Range Nanomolar to millimolar 0.5 ppb-500 ppm
Repeatability +/- 5% +/- 5%
Sample Volume: 0.001–1 mL Flow rates: 10-200 mL/min

General

Response Time Electronics: 67 milliseconds to 90% full scale. Lagtime: 1 second
Outputs (1) analog 0-1V, 0-10V (1) digital RS-232 (9600-38400 Baud), (1) printer parallel port
Data Sampling Rate Operator selectable — 32, 16, 8, 4, 2,or 1 samples per second; or 12, 4, 2, 1, 0.2, or

0.1 samples per minute

Power Requirements U.S. — 110/115V, 60 Hz (6A)

Japan — 90/100V, 50/60 Hz (7A)
Europe, Asia and South America — 220/240V 50 Hz (3A)

Dimensions

NOA 280i 41 H x 16 W x 51 L cm (16 H x 6.2 W x 20 L in)

Weight: 16 kg (35 lb)

Vacuum Pump 37 H x 19 W x 48 L cm (14.5 H x 7.5 W x 19 L in)

Weight: 21.5 kg (47 lb)

* Trademark of General Electric Company; may be registered in one or more countries.

1

US Patent Numbers: 5,795,787 and 6,010,459. Under license from Aperon Biosystems, Inc.

Caution: For Research Use Only. Not for Use in Diagnostic Procedures.

The Americas

GE Analytical Instruments
6060 Spine Road
Boulder, CO 80301-3687 USA
T [phone]
T [phone]
F [phone]
[e-mail]

Europe/Middle East/Africa

GE Analytical Instruments
Unit 3, Mercury Way
Urmston, Manchester
UK M41 7LY
T [phone]
F [phone]
[e-mail]

Asia Pacific

GE Analytical Instruments
5 United Plaza
1468 Nanjing Rd (W)
Shanghai 200040
China
T [phone]
[e-mail]

www.geinstruments.com

©2008, General Electric Company. All rights reserved.

30000089RevB, Jun-08

MC08-131