GenScript eStain User Manual
Version 2.0
June 4, 2010
Prot e in Staining System
User Manual
For electric staining of proteins in mini polyacrylamide gels
The Biology CRO
Table of Contents
Product Contents
Product Specifications
eStain
TM
Protein Staining Device
Accessory Products
Introduction
Quick Reference Guide
Protocols
Troubleshooting
Examples of Results
Technical Support
Warranty
1
3
5
7
8
11
12
21
22
23
24
eStainTM Protein Staining System www.genscript.com
Product Contents Product Contents,
Type of products
eStain
TM
Protein Staining
Device Contents
Upon Receiving
the Instrument
eStain
TM
Protein Staining
Pads
Product
eStainTM Protein Staining Device
Cat. No.
L02010
Component
eStainTM Protein Staining Device
Specific Power Cord based on the type
of unit ordered (for U.S./Canada/China/
Taiwan/Japan, Europe, or UK)
Forceps
Shovel
eStainTM Graphite Electrode (11mm)
(installed inside the device)
Ice Pack
Quantity
1 each
1 each
1 each
1 each
1 each
1 each
Product
eStainTM Protein Staining Pads (R-250, 20-pak)
eStainTM Protein Staining Pads (G-250, 20-pak)
Cat. No.
L02011
L02012
This manual is supplied with the following products:
The contents of the eStainTM Protein Staining Device are listed below
Examine the unit carefully for any damage incurred during transit. File any
damage claims with the carrier. The warranty does not cover in-transit
damage.
The following eStain
TM
Protein Staining Pads are available from GenScript:
If you ordered the eStainTM Protein Staining Pads, you will receive the
components listed in the table below. Store the eStainTM Protein Staining
Pads at room temperature. For best results, use the eStainTM Staining Pads
before the expiration date printed on the package.
eStainTM Protein Staining System www.genscript.com eStain
continued
eStain
TM
Protein Staining
Pads, continued
eStainTM Graphite
Electrode
Product
eStainTM Graphite Electrode (11mm, 1-pak)
Cat. No.
L02014
The following eStainTM Graphite Electrode is available from GenScript:
For best results, when a Graphite Electrode was used for 200 times of electric
staining, replace it with a new one.
Component
The eStain
TM
Protein Staining Pads
(R-250, 20-pak) contains:
eStainTM Protein Staining Pad (R-250)
Gel Window (66mm×56mm)
Gel Window (76mm×64mm)
The eStainTM Protein Staining Pads
(G-250, 20-pak) contains:
eStainTM Protein Staining Pad (G-250)
Gel Window (66mm×56mm)
Gel Window (76mm×64mm)
each pack eStain
TM
Protein Staining Pad
(R-250) contains:
1×eStainTM Cathode Pad (R-250)
1×eStainTM Anode Pad
each pack eStainTM Protein Staining Pad
(G-250) contains:
1×eStainTM Cathode Pad (G-250)
1×eStainTM Anode Pad
TM
Protein Staining System www.genscript.com
Quantity
20
1
1
20
1
1
1
1
1
1
- 2 --1 -
Product Specifications Product Specifications,
eStainTM Protein Staining
Device Specifications
- 3 -
Weight:
Dimensions:
Electrical Parameters:
Built-in Features:
Compatibility:
Materials:
Operating Temperature:
Forceps:
Shovel:
2.3 kg
200 mm (l) ×180 mm (w) ×90 mm (h)
100-120 V, 220-240 V, 50/60 Hz, 3.2 A
Digital display, alarm, light LED
Suitable for electric staining of proteins in mini
polyacrylamide gels
Acrylonitrile Butadiene Styrene, Polycarbonate,
Aluminum, Titanium, Plasticized silicone.
15 - 40°C
Stainless steel
Polycarbonate
eStainTM Protein Staining
Pads Specifications
size:
Materials:
8cm (l) x 7cm (w) x2.5mm (thick)
Blotting filter paper presoaked with proprietary
cathode buffer containing CBB R-250 or G-250
size:
Materials:
8cm (l) x 7cm (w) x2.5mm (thick)
Blotting filter paper presoaked with proprietary
anode buffer
The eStainTM Protein Staining Pads are used with the eStainTM Protein Staining
Device.
The specifications of the eStainTM Protein Staining Pads are listed below:
eStainTM Cathode Pad (R-250 or G-250)
Avoid acetone, dimethyl sulfoxide, and acetic acid. These reagents can erode or
damage the device.
eStainTM Anode Pad
Inter frame size:
Materials:
66mm×56mm
76mm×64mm
Polycarbonate
Gel Window
eStainTM Protein Staining System www.genscript.com eStain
- 4 -
continued
eStainTM Graphite
Electrode Specifications
Dimensions:
Weight:
Materials:
100 mm (l) ×90 mm (w) ×11mm (h)
180g
Powdered carbon, clay
eStain
TM
Graphite Electrode (11mm)
The eStain
TM
Graphite Electrode is used as the replaceable anode of eStain
TM
Protein Staining Device and available separately from GenScript.
The specifications for eStainTM Graphite Electrode are listed below:
TM
Protein Staining System www.genscript.com
eStainTM Protein Staining Device eStain
Front View of
eStain
TM
Device
Rear View of
eStain
TM
Device
- 5 -
The rear view showing various parts of the eStain
TM
Protein Staining Device
is shown below.
The front top view showing various parts of the eStain
TM
Protein Staining Device
is shown below.
Control Panel
Handle Bar
eStainTM Protein Staining System www.genscript.com eStain
Power Switch AC Inlet
- 6 -
TM
Protein Staining Device,
continued
Control Panel of
eStain
TM
Device
Inside View of
eStain
TM
Device
The control panel of the eStainTM Protein Staining Device is described below.
When upper status light is on, which indicates staining mode, the Digital
Display shows running time; otherwise when both upper and lower status lights
are on, which indicates numbering mode, the Digital Display shows how many
times of electric staining the eStainTM Graphite Electrode has been used for.
The Reset button is used to clear parameters.
The Time button is used to set running time and toggle between staining mode
and numbering mode.
The Start/Stop button is used to activate/stop the staining program.
The inside view showing various parts of the eStain
TM
Protein Staining
Device is shown below:
TM
Protein Staining System www.genscript.com
Titanium Cathode
Graphite Anode
Accessory Products Introduction
eStainTM Protein Staining System www.genscript.com
eStainTM Protein Staining
Pads
eStain
TM
Graphite
Electrode
Additional Products
- 7 -
eStain
TM
Graphite Electrode is available separately from GenScript. Ordering
information is provided below.
The precast Express
TM
PAGE Gels as well as premade buffers or buffer powders
are available from GenScript. For details, contact Technical Support or visit
www.genscript.com.
eStain
TM
Protein Staining Pads are available separately from GenScript. Ordering
information is provided below.
Product
eStainTM Protein Staining Pads
(R-250, 20-pak)
eStainTM Protein Staining Pads
(G-250, 20-pak)
Cat. No.
L02011
L02012
Quantity
1 box
1 box
Product
eStainTM Graphite Electrode (11mm, 1-pak)
Cat. No.
L02014
Quantity
1 unit
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