GeneCopoeia AOPR-0200, AOPR-0600, AOPR-1200 User Guide
All‐in‐One
Foruniversalquantitativereal‐timePCR
Cat.No.AOPR‐0200(200qPCRreactions)
Cat.No.AOPR‐0600(600qPCRreactions)
Cat.No.AOPR‐1200(1200qPCRreactions)
PerformanceoptimizedwithAll‐In‐One™qPCRPrimersandAll‐in‐OneFirst‐StrandcDNA
SynthesisKit
™
qPCRMix
UserManualI
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USA
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© 2009 GeneCopoeia, Inc.
All-in-One™ qPCR Mix Manual
USER MANUAL I
™
All-in-One
I. Description
II. Related Products
III. Contents and Storage
IV. Preparation
V. Procedure
VI. Example
VII. Trouble Shooting Guide
VIII. Limited Use License and Warranty
I. Description
The All-in-One™ qPCR Mix provides fast and efficient SYBR
qPCR Mix uses a high-fidelity hot-start DNA polymerase, optimized reaction buffer and high-quality dNTPs
to enable specific and sensitive amplification of even low-copy genes. The All-in-One qPCR Mix reduces
experimental design time by providing a universal reaction condition that can be used with almost all primers
and most real-time PCR instruments.
II. Related Products
GeneCopoeia offers comprehensive solutions for studying gene expression. A careful process of co
develop-ment ensures that they work well together and provide robust and reproducible results.
qPCR Mix
®
Green-based real-time quantitative PCR. The
GeneCopoeia Description
All-in-One™ First-Strand cDNA
Synthesis Kit
All-in-One™ qPCR Primers
All-in-One™ qPCR Primer Array
All-in-One™ miRNA qRT-PCR
Detection Kits
All-in-One™ miRNA qPCR Primers
OmicsLink™ Expression-Ready
ORF cDNA Clones
Endofectin™ Transfection Reagents
Reverse transcription kit
Human, mouse and rat
primers
User specified, ready-to-use
primer arrays in 96-wellplate format
®
SYBR
Green-based Accurately quantify miRNA expression
Human, mouse and rat
primers
20,000 human
15,000 mouse
Optimized for specific cell
types
Produces first-strand cDNA using poly A
or total RNA as template
Validated, gene-specific primers ensure
specificity and sensitivity
Reliable tools ideal for analyzing the
expression of a focused panel of genes
such as pathways, diseases or
customized gene panels
Validated for robust, reproducible and
reliable quantitation of miRNA activity
Perform a variety of applications with
expression-ready clones
Transfect efficiently and with low toxicity
2
All-in-One™ qPCR Mix Manual
III. Contents and Storage
Contents and storage recommendations for the All-in-One qPCR Mix
Cat. Nos. AOPR-0200, AOPR-0600
Contents Quantity Storage temperature/ conditions
2X All-in-One qPCR Mix
50X ROX Reference Dye
and AOPR-1200 are provided in the following table.
2 x 1 ml
3 x (2 x 1 ml)
6 x (2 x 1 ml)
1 x 80 µl
3 x 80 µl
6 x 80 µl
–20°C (Stable for at least 12 months)
Alternatively, the solution can also be
stored at -80°C in aliquots. Avoid
repeated freezing/ thawing.
–20°C (Stable for at least 12 months)
Alternatively, the solution can also be
stored at -80 °C in aliquots. Avoid
repeated freezing/ thawing.
IV. Preparation
Wearing a lab coat, disposable gloves and protective goggles are recommended when handling
chemicals.
IMPORTANT NOTES:
1. Store the kit at –20°C. Avoid storage or leaving reagents at 4°C or room temperature. Avoid light
exposure at all times.
2. Mix reagents thoroughly by gently inverting tubes several times avoiding bubbles and then briefly
centrifuge before use.
3. Prepare the reaction mix with PCR grade water.
4. Strictly follow standard procedures for PCR to avoid nucleic acid contamination and non-specific
amplification.
5. Read all procedures before setting up the PCR reaction
V. Procedure
1. Thaw the 2X All-in-One qPCR Mix and 50X ROX Reference Dye as needed.
2. Prepare the PCR reaction mix on ice. See the example below.
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