GE TYPHOON FLA 7000 Getting Started Manual

Getting Started with

Typhoon

™

FLA 7000

Original instructions

Table of Contents

41 Introduction ..........................................................................................................

51.1 Important user information .............................................................................................................

71.2 Regulatory information ......................................................................................................................

111.3 The Typhoon FLA 7000 laser scanner .........................................................................................

141.4 Typhoon FLA 7000 control software ............................................................................................

182 Safety instructions ...............................................................................................

192.1 Safety precautions ...............................................................................................................................

252.2 Labels .........................................................................................................................................................

302.3 Emergency procedure ........................................................................................................................

312.4 Recycling information .........................................................................................................................

322.5 Declaration of Hazardous Substances (DoHS) ........................................................................

343 Installation ............................................................................................................

353.1 Site requirements ..................................................................................................................................

363.2 Transporting Typhoon FLA 7000 ...................................................................................................

373.3 Delivery inspection ...............................................................................................................................

383.4 Connections ............................................................................................................................................

393.5 Software installation ...........................................................................................................................

403.5.1 Installing Typhoon FLA 7000 Control Software for Windows XP ..............................

443.5.2 Installing Typhoon FLA 7000 Control Software for Windows Vista .........................

483.5.3 Installing 32-bit Typhoon FLA 7000 Control Software for Windows 7 ...................

583.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 ....................

703.6 Preparations before starting Typhoon FLA 7000 ...................................................................

714 Operation ..............................................................................................................

724.1 Operation overview .............................................................................................................................

734.2 Starting the instrument and the Typhoon FLA 7000 control software ........................

765 Reading fluorescent samples ............................................................................

775.1 Set the reading mode and reading conditions .......................................................................

795.2 Place the sample on the stage .......................................................................................................

825.3 Place the stage in Typhoon FLA 7000 .........................................................................................

835.4 Read the sample ...................................................................................................................................

846 Exposing Storage phosphor screens ................................................................

856.1 Handling precautions .........................................................................................................................

876.2 Preparing the Storage phosphor screen ...................................................................................

896.3 Exposing the Storage phosphor screen .....................................................................................

917 Reading phosphorimaging samples .................................................................

927.1 Set the reading conditions ...............................................................................................................

957.2 Place the storage phosphor screen on the phosphor stage ............................................

967.3 Place the phosphor stage in the Typhoon FLA 7000 ...........................................................

2 Getting Started with Typhoon FLA 7000 28-9607-64 AD

Table of Contents

977.4 Read the sample ...................................................................................................................................

988 Reading digitization samples .............................................................................

998.1 Set the reading mode and reading conditions .......................................................................

1018.2 Place the sample on the stage .......................................................................................................

1028.3 Place the stage in the Typhoon FLA 7000 .................................................................................

1038.4 Read the sample ...................................................................................................................................

104Appendix A Other settings .....................................................................................

106Appendix B Daily maintenance ............................................................................

107Appendix C Periodic maintenance .......................................................................

108Appendix D Installing and replacing filters ........................................................

113Appendix E Default sample detection methods ................................................

115Appendix F Specifications .....................................................................................

Getting Started with Typhoon FLA 7000 28-9607-64 AD 3

Table of Contents

1 Introduction

See pageSection

51.1 Important user information

71.2 Regulatory information

111.3 The Typhoon FLA 7000 laser scanner

141.4 Typhoon FLA 7000 control software

Purpose of Getting Started

This manual provides you with the instructions needed to handle the Typhoon FLA 7000
system in a safe way.

Prerequisites

In order to operate the Typhoon FLA 7000 in the way it is intended, the followingprereq­uisites must be fulfilled:

•

You have read and understood the safety instructions outlined in this manual.

•

The instrument is installed according to the instructions in this manual.

In this chapter

This chapter contains important user information, and a general description of the
Typhoon FLA 7000 and its intended use.

4 Getting Started with Typhoon FLA 7000 28-9607-64 AD

1 Introduction

1.1 Important user information

Read this before using Typhoon
FLA 7000

All users must read this entire manual to fully understand the safe use of Typhoon FLA

7000.

Intended use

Typhoon FLA 7000 is a fast laser scanner optimized for quantitative phosphorimaging,
Amersham ECL Plex™ Western blots, visible fluorescence and gel documentation.

Typhoon FLA 7000 is intended for research use only, and shall not be used in anyclinical
procedures, or for diagnostic purposes.

Safety notices

This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.

WARNING

WARNING indicates a hazardous situation which, if not avoided,

could result in death orserious injury. It is important not to proceed
until all stated conditions are met and clearly understood.

CAUTION

CAUTION indicates a hazardous situation which, if not avoided,

could result in minor or moderate injury. It is important not to pro­ceed until all stated conditions are met and clearly understood.

NOTICE

NOTICE indicates instructions that must be followed to avoid

damage to the product or other equipment.

Getting Started with Typhoon FLA 7000 28-9607-64 AD 5

1 Introduction

1.1 Important user information

Notes and tips

A note is used to indicate information that is important for trouble-free and
optimal use of the product .

Note:

A tip contains useful information that can improve or optimize your procedures.

Tip:

Typographical conventions

Software items areidentified in thetext by bolditalic text. A colon separatesmenu levels,
thus File:Open refers to the Open command in the File menu.

Hardware items are identified in the text by bold text (for example, Power).

6 Getting Started with Typhoon FLA 7000 28-9607-64 AD

1 Introduction

1.1 Important user information

1.2 Regulatory information

This section lists the directives and standards that are fulfilled by the Typhoon FLA 7000
system.

Manufacturing information

The table below summarizes the required manufacturing information. For further infor­mation, see the EU Declaration of Conformity document.

ContentRequirements

GE Healthcare Bio-Sciences AB, Björkgatan
30, SE 751 84 Uppsala, Sweden

Name and address of manufacturer

INTERTEK SEMKO AB, NB 0413Name and ID of notified body

Conformity with EU Directives

This product complies with the European directives listed in the table, by fulfilling the
corresponding harmonized standards.

TitleDirective

Machinery Directive (MD)2006/42/EC

Electromagnetic Compatibility (EMC) Directive2004/108/EC

Low Voltage Directive (LVD)2006/95/EC

International standards

This product fulfills the requirements of the following standards:

NotesDescriptionStandard

EN 61010-1 harmo­nized with EU directive
2006/95/EC

Safety requirements for electri­cal equipment for measure­ment, control, and laboratory
use

EN/IEC 61010-1, UL
61010-1, IEC 61010-2­081, CAN/CSA-C22.2
No. 61010-1

EN 61326-1 harmo­nized with EU directive
2004/108/EC

EMC emissions and immunity
requirements for electrical
equipment for measurement,
control and laboratory use

EN 61326-1
VCCI Class A
FCC Part 15 B Class A
ICES-003 Class A

Getting Started with Typhoon FLA 7000 28-9607-64 AD 7

1 Introduction

1.1 Important user information

NotesDescriptionStandard

EN ISO standard is har­monized with EU direc­tive 2006/42/EC

Safety of machinery, general
principles for design, risk as­sessment and risk reduction

EN ISO 12100

EN standard harmo­nized with 2006/95/EC

Safety of laser productsEN/IEC 60825-1

Safety of laser productsUSA 21 CFR, Chapter I,

Subchapter J, Part

1040.10 Laser Products

CE marking

The CE marking and the corresponding EU Declaration of Conformity is valid for the in­strument when it is:

•

used as a stand-alone unit, or

•

connected to otherproducts recommended or describedin theuser documentation,
and

•

used in the same state as it was delivered from GE, except for alterations described
in the user documentation.

FCC compliance

This device complies with part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) This device may not cause harmful interference, and (2) this device
must accept anyinterference received,including interferencethat may cause undesired
operation.

The user is cautioned that any changes or modifications not expressly approved
by GE could void the user’s authority to operate the equipment.

Note:

8 Getting Started with Typhoon FLA 7000 28-9607-64 AD

1 Introduction

1.2 Regulatory information

This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea­sonable protection against harmful interference when the equipment is operated in a
commercial environment. Thisequipment generates, uses, andcan radiate radio frequen­cy energy and, if not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user will be re­quired to correct the interference at his own expense.

Environmental requirement

TitleRequirement

Restriction of Hazardous Substances (RoHS) Directive2011/65/EU

Waste Electricaland Electronic Equipment (WEEE)Directive2012/19/EU

Administration on the Control of Pollution Caused by Elec­tronic Information Products,China Restriction of Hazardous
Substances (RoHS)

ACPEIP

Registration, Evaluation, Authorization and restriction of
CHemicals (REACH)

Regulation (EC) No
1907/2006

Laser standards

This instrument is a Class 1 (IEC60825-1:2007/EN60825-1:2007) laser product.

Getting Started with Typhoon FLA 7000 28-9607-64 AD 9

1 Introduction

1.2 Regulatory information

The following lasers can be installed in Typhoon FLA 7000:

Maximum powerWavelengthLaser and class

20 mW (CW)473 nmLD laser, class 3B

10 mW (CW)532 nmSHG laser, class 3B

45 mW (CW)635 nmLD laser, class 3B

80 mW (CW)650 nmLD laser, class 3B

The beam divergence of all laser modules is collimated.

Note:

Regulatory compliance of
connected equipment

Any equipment connected to Typhoon FLA 7000 should meet the safety requirements
of EN/IEC 61010-1, or relevant harmonized standards. Within EU, connected equipment
must be CE marked.

10 Getting Started with Typhoon FLA 7000 28-9607-64 AD

1 Introduction

1.2 Regulatory information

1.3 The Typhoon FLA 7000 laser scanner

Major features

The compact Typhoon FLA 7000 is an image analyzer that offers high performance,
multifunctionality, and high-speed reading. Benefits include:

•

Detection of high-sensitivity autoradiographs using storage phosphor screens.

•

Four lasers and multiple filters enable the use of a variety of fluorescent dyes and
detection methods.

•

Data is aquired through high resolution, sensitivity and wide range linearity.

•

Compatibility with various types of samples, for example fluorescent labels, fluores­cent dyes, CBB stain, silver stain, X-ray film, and storage phosphor screens.

Getting Started with Typhoon FLA 7000 28-9607-64 AD 11

1 Introduction

1.3 The Typhoon FLA 7000 laser scanner

Parts and functions

1

2

3

4

5

6

7

8

9

A

B

C

D

E F G H

I J

K

L

M

N O

P

A

B

C

D

E

F G

H

I

J K L

M N

O

P

A B C D

E

F G H I

J

K

L

M N O

P

A B C D E

F

G H

I J K

L

M

N O P

1

2

3

4

5

6

7

8

9

FunctionPart

Power switch, located between the AC connector and the USB connector1

Indicator lamps2

Knob3

Lid4

Setting block5

Filter change door6

Filter module slot7

Filter module8

Filter9

12 Getting Started with Typhoon FLA 7000 28-9607-64 AD

1 Introduction

1.3 The Typhoon FLA 7000 laser scanner

FunctionPart

Phosphor Stage10

Suction rod11

Protection cover for storage phosphor screen12

Fluor Stage13

Digitizing fluorescent plate14

Membrane weight (included in Fluor stage)15

Getting Started with Typhoon FLA 7000 28-9607-64 AD 13

1 Introduction

1.3 The Typhoon FLA 7000 laser scanner

1.4 Typhoon FLA 7000 control software

System requirements

Minimum requirementParameter

Microsoft®Windows®XP Professional SP3 (32-bit)Operating system

or

Microsoft Windows Vista®Business SP2 (32-bit)

or

Microsoft Windows 7 Professional (32-bit)

or

Microsoft Windows 7 Professional (64-bit)

1 GBInternal memory

Intel®Core 2 Duo processorProcessor

80 GBHard drive

1280 × 1024 pixelsMonitor resolution

One USB 2.0 portOther requirements

DVD-ROM drive

14 Getting Started with Typhoon FLA 7000 28-9607-64 AD

1 Introduction

1.4 Typhoon FLA 7000 control software

Overview of the main window

The Typhoon FLA 7000 is used to control, use and supervise the Typhoon FLA 7000.

1

2

3

4

5

6 7 8

FunctionPart

Phosphorimaging button: click to read a storage phosphor screen1

Fluorescence button: click to read a fluorescent sample2

Digitization button: click to perform digitization3

Method button: click to register or erase a combination of laser and filter.4

Filter Module button: click to change or register a filter.5

Preferences button: clickto set theimage file format, scan mode, correction

mode and to enable or disable the ND filter.

6

Filter: displays the loaded filters.7

Laser: displays the status of the loaded laser units8

Getting Started with Typhoon FLA 7000 28-9607-64 AD 15

1 Introduction

1.4 Typhoon FLA 7000 control software

Overview of the reader settings
window

1

2

3

4

7

6

12

13

10

11

8

5

9

14

15

DescriptionPart

Image folder: specify where to save the file after the reading.1

File Name: enter the name of a file to save image data.2

Comment: enter an optional comment. Thecomment is embedded

in the file where the image is saved, and can be viewed with the
analyzing software.

3

Method: set the method to use in the scan. Up to 4 scans can be
performed in a row, all with individual methods.

4

PMT: set the voltage of the photo-multiplier tube. The higher the
value, the higher the sensitivity.

5

Click to increase the number of scans

Click to decrease the number of scans

16 Getting Started with Typhoon FLA 7000 28-9607-64 AD

1 Introduction

1.4 Typhoon FLA 7000 control software

DescriptionPart

Set the scanning area.6

1

Select a stage in the drop-down menu.

2

Drag the red square to the desired position of the scanning
area.

3

Drag the sides of the red square as needed to adjust the size
of the scanning area.

Pixel Size: set the pixel size.7

Choose asmall pixel size for highquality images. Note that a small
pixel size setting increases the reading time and the size of the
image file.

Latitude: Specify the dynamic range.8

•

L5 provides a larger detectable range than L4.

•

L4 provides a finer densitygradation, if thesignals of thesample
areas are in the correct range.

Mode: Specify the mode for the reading area borders.9

•

Select Grid to limit the borders to the grid lines.

•

Select Free for an arbitrary area.

•

Select All to use the entire Fluor stage as the reading area.

Save Condition... click this button to save the current reading
conditions in a file, if desired. For details, refer to the User Manual.

10

Load Condition... click this button to loadpreviously savedreading
conditions, if desired. For details, refer to the User Manual.

11

File Size: the estimated size of the result data file is presented.12

Reading Time: the estimatedtime required for the scan is present-

ed.

13

Top: return to the main window.14

Start Scan: start the scan. The sample must be loaded before

starting a scan.

15

Getting Started with Typhoon FLA 7000 28-9607-64 AD 17

1 Introduction

1.4 Typhoon FLA 7000 control software

2 Safety instructions

In this chapter

This chapter contains the following sections:

See pageSection

192.1 Safety precautions

252.2 Labels

302.3 Emergency procedure

312.4 Recycling information

322.5 Declaration of Hazardous Substances (DoHS)

18 Getting Started with Typhoon FLA 7000 28-9607-64 AD

2 Safety instructions

2.1 Safety precautions

Introduction

The Typhoon FLA 7000 is powered by mains voltage and is used to image samples that
may be hazardous. Before installing, operating or maintaining the equipment, you must
be aware of the hazards described in the user documentation. Follow the instructions
provided to avoid personal injury or damage to the equipment.

General precautions

WARNING

Do notuse the equipment if smoke, strangenoises or strange odors
can be perceived, or if the equipment becomes unusually hot. This
may result in fire or electric shock.

Stop using the equipment immediately, turn off the power switch
and unplug the equipment from the power outlet. Contact your
local GE representative to request repair.

WARNING

Do notdamage the power supplycord by bending, twisting,heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.

If the power supply cords are damaged, contact your local GE
representative for replacements.

WARNING

Do not place the equipment on unstable tables or on inclined sur­faces, as the equipment could be dropped orfall, resultingin injury.

Getting Started with Typhoon FLA 7000 28-9607-64 AD 19

2 Safety instructions

2.1 Safety precautions

WARNING

Do notallow liquids, flammable materialsor metallic objects to get
into the Typhoon FLA 7000. This may result in fire or electric shock.

Turn off the power switch, unplug the equipment from the power
outlet, then contact you local GE representative.

CAUTION

Do not scratchor drop parts containingglass such as lenses, filters
or lights.

Laser safety

WARNING

Never detach the inner cover screwed to this instrument. If it is
detached, laser beam may leak with a risk of loss of vision.

CAUTION

Never cancelthe interlocks in this instrument, laserbeam may leak
with a risk of loss of vision.

CAUTION

Using procedures or adjustments other thanthose specified in this
manual may result in hazardous exposure to laser radiation.

20 Getting Started with Typhoon FLA 7000 28-9607-64 AD

2 Safety instructions

2.1 Safety precautions

Radiation safety

This instrument is not equipped with any radioisotope or radiation generating unit, and
is therefore not regulated by radiation hazardprevention laws. However, the instrument
is capableof reading Storage phosphorscreens which may bepolluted by radioisotopes.

CAUTION

If radioisotope (RI) pollution occurs, stop use of the instrument im­mediately and follow the instructionsof your radiation administra­tor.

Personal protection

CAUTION

Always wear gloves, protective glasses and a lab coat or similar
when handling samples.

CAUTION

Always wear cotton gloves when handling Storage phosphor
screens.

CAUTION

Wear gloves, protective glasses and a lab coat or similar when
disposing of the Typhoon FLA 7000.

Installing and moving the
instrument

WARNING

The Typhoon FLA 7000 instrument must always be connected to
a grounded power outlet.

Getting Started with Typhoon FLA 7000 28-9607-64 AD 21

2 Safety instructions

2.1 Safety precautions

WARNING

Do not block the ventilation inlets or outlets on the system.

WARNING

Power cord. Only use power cords with approved plugs delivered

or approved by GE.

WARNING

Heavy object. Because of the significant weight of Typhoon FLA

7000, great care must be taken not to cause squeeze or crushing
injuries during movement. Use suitable lifting equipment when
moving the unit.

CAUTION

•

Do not connect any USB devices other than the Typhoon FLA
7000 to the computer in which the Typhoon FLA 7000 Control
Software is installed.

•

Use only the Typhoon FLA 7000 Control Software during read­ing.

System operation

WARNING

Do not use the equipment with a power supply other than that
recommended. Fire and electric shock could result.

WARNING

Do notuse the equipmentwithin or near a sink, or in humid or dusty
environments. Fire and electric shock could result.

22 Getting Started with Typhoon FLA 7000 28-9607-64 AD

2 Safety instructions

2.1 Safety precautions

WARNING

Connect thepower supply directly toa grounded wall poweroutlet.
The useof extension cords or multiple loads onone electrical outlet
could result in fire and electric shock.

CAUTION

Do not usethe same power supply asthat of large equipment such
as an air conditioner or centrifuge. Malfunction could result.

NOTICE

Avoid exposing storage phosphor screens in places where the en­vironmental radiation may be increased, for example rooms with
concrete walls or in basements.

Maintenance

WARNING

Do not attempt to modify the equipment, or fire and electric shock
could result.

WARNING

Do not use excessive amounts of liquids for cleaning the Typhoon
FLA 7000, this may result in product malfunction or electric shock.

CAUTION

Wear gloves to prevent direct contact with chemical substances.

Getting Started with Typhoon FLA 7000 28-9607-64 AD 23

2 Safety instructions

2.1 Safety precautions

CAUTION

Take care when connecting the power supply cable. Do not tug on
the cable,and do not handle the connectionplugs with wet hands.

CAUTION

Connect the computerhardware on thesame powercircuit, other­wise the equipment may be influenced by electrical nosie.

CAUTION

Turn off the power switch and remove connecting cables before
moving the equipment.

CAUTION

Turn the power switch off before cleaning the inside of the equip­ment.

CAUTION

Unplug the equipment if it will not be used for an extended period.

24 Getting Started with Typhoon FLA 7000 28-9607-64 AD

2 Safety instructions

2.1 Safety precautions

2.2 Labels

Typhoon FLA 7000 serial number

The Typhoon FLA 7000 serial number islocated on alabel on theback of theinstrument.

Symbols used in safety labels

MeaningLabel

Warning! Read the user documentation before using the system. Do

not open any covers or replace parts unless specifically stated in the
user documentation.

The systemcomplies with the requirements forelectromagnetic compli­ance (EMC) in Australia and New Zealand.

The system complies with applicable European directives.

Getting Started with Typhoon FLA 7000 28-9607-64 AD 25

2 Safety instructions

2.2 Labels

Labels concerning use of
hazardous substances

MeaningLabel

This symbolindicates that the waste of electricaland electronic equipment
must not be disposed as unsorted municipal waste and must be collected
separately. Please contact an authorized representative of the manufac­turer for information concerning the decommissioning of equipment.

This symbol indicates that the product contains hazardous materials in
excess of the limits established by the Chinese standard SJ/T11363-2006
Requirements for Concentration Limits for Certain Hazardous Substances
in Electronics.

Labels concerning laser light

MeaningLabel

CAUTION! Avoid exposure to the laser

beam when the lid is open.

CAUTION! Class 3B Laser product when
open andinterlock defeated. Avoid expo­sure to the beam.

Do notattempt to defeatthe safety inter­locks under the sample lid or behind the
filter door, orotherwise try to gainaccess
to the interior of the instrument through
any otheropening. Exposure to laserlight
can cause injury. Viewing the laser light
directly can cause blindness.

26 Getting Started with Typhoon FLA 7000 28-9607-64 AD

2 Safety instructions

2.2 Labels

Labels at the front of Typhoon
FLA 7000

Getting Started with Typhoon FLA 7000 28-9607-64 AD 27

2 Safety instructions

2.2 Labels

Labels at rear of Typhoon FLA
7000

28 Getting Started with Typhoon FLA 7000 28-9607-64 AD

2 Safety instructions

2.2 Labels

Labels on left side of Typhoon
FLA 7000

I : Power ON
O : Power OFF

USB

Getting Started with Typhoon FLA 7000 28-9607-64 AD 29

2 Safety instructions

2.2 Labels

2.3 Emergency procedure

This section describes how to do an emergency shutdown of the Typhoon FLA 7000 in­strument. The section also describes the results of a power failure.

Emergency shutdown

ActionStep

Click the Stop button in the Typhoon FLA 7000.1

Turn off the Typhoon FLA 7000 by pressing the power switch on the left side
of the instrument body.

2

Disconnect the power cord from the power outlet.3

Power failure

The results of a power failure depends on the unit or units affected.

ResultsUnit affected by power fail-

ure

•

The reading is interrupted immediately. The in­strument is in an undefined state.

•

The data collected up to the time of the power
failure is available in the file created when
starting the scan.

Typhoon FLA 7000 instrument

•

The computer shuts down immediately.

•

The run continues, but no data is saved.

Computer runningthe control
software

30 Getting Started with Typhoon FLA 7000 28-9607-64 AD

2 Safety instructions

2.3 Emergency procedure