SE 250/260 Mighty Small II
Operating Instructions
Original instructions
Table of Contents
31 Introduction ..........................................................................................................
41.1 About this manual ................................................................................................................................
51.2 Important user information .............................................................................................................
71.3 Regulatory information ......................................................................................................................
81.3.1 EU directives .....................................................................................................................................
91.3.2 Eurasian Customs Union ............................................................................................................
101.3.3 Regulations for USA and Canada ...........................................................................................
111.3.4 Other regulations and standards ............................................................................................
122 Safety information ...............................................................................................
132.1 Safety precautions ...............................................................................................................................
202.2 Labels .........................................................................................................................................................
222.3 Emergency procedures ......................................................................................................................
232.4 Recycling information .........................................................................................................................
242.5 Declaration of Hazardous Substances (DoHS) ........................................................................
263 System description ..............................................................................................
284 Installation ............................................................................................................
295 Operation ..............................................................................................................
315.1 Preparations ............................................................................................................................................
345.2 Assembly ..................................................................................................................................................
405.3 Electrophoresis run ..............................................................................................................................
426 Maintenance .........................................................................................................
447 Troubleshooting ...................................................................................................
478 Reference information ........................................................................................
488.1 Specifications .........................................................................................................................................
498.2 Ordering information ..........................................................................................................................
508.3 Health and Safety Declaration Forms .........................................................................................
52Index .......................................................................................................................
2 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
Table of Contents
1 Introduction
About this chapter
This chaptercontains importantuser information,descriptions ofsafety notices,regulatory information, and intended use of the SE 250/260 Mighty Small II.
In this chapter
See pageSection
41.1 About this manual
51.2 Important user information
71.3 Regulatory information
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 3
1 Introduction
1.1 About this manual
Purpose of this manual
The Operating instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions covers the SE 250/260 Mighty Small II. The illustration below
shows the SE 250/260 Mighty Small II.
Illustrations
The images and annotations in this document are for illustrative purposes only. The
configuration of individual products mayvary, andtherefore illustrations maynot reflect
the actual system delivered.
4 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
1 Introduction
1.1 About this manual
1.2 Important user information
Read this before operating the
product
All users must read the entire Operating instruction before installing, operating or
maintaining the product.
Always keep the Operating instruction at hand when operating the product.
Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use of the product
The SE 250/260 Mighty Small II small format vertical slab gel unit is intended for rapid
electrophoresis of proteinor nucleicacid samples.Most samplescan berun in 45 minutes,
and only a minimal amount of sample is required.
The SE 250/260 Mighty Small II accommodates one or two 10 × 8 cm gel sandwiches.
The SE 250/260 Mighty Small II accommodates one or two 10 × 8 cm or 10 × 10.5 cm
gel sandwiches. In both SE 250/260 Mighty Small II units, the upper buffer chamber is
formed when the notched side of a gel sandwich is sealed against the silicone rubber
gasket.
The upper buffer chamber core serves as a heat exchanger if cooling is required. The
core is hollow and equipped with ports on either side for coolant circulation.
Prerequisites
In order to operate the SE 250/260 Mighty Small II in the way it is intended:
•
The user must have a general understanding of gel electrophoresis.
•
The user must read and understand theSafety Instructions chapter in the Operating
Instructions.
•
The SE 250/260 Mighty Small II must be installed in accordance with the instructions
in the Operating Instructions.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 5
1 Introduction
1.2 Important user information
Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result indeath or serious injury. It is importantnot to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Note:
A tip contains useful information that can improve or optimize your procedures.
Tip:
6 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
1 Introduction
1.2 Important user information
1.3 Regulatory information
Introduction
This section lists the regulations and standards that apply to the SE 250/260 Mighty
Small II.
Manufacturing information
The table below summarizes the required manufacturing information.
InformationRequirement
GE Healthcare Bio-Sciences AB,Name and address of manufacturer
Björkgatan 30, SE 751 84 Uppsala, Sweden
In this section
See pageSection
81.3.1 EU directives
91.3.2 Eurasian Customs Union
101.3.3 Regulations for USA and Canada
111.3.4 Other regulations and standards
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 7
1 Introduction
1.3 Regulatory information
1.3.1 EU directives
Introduction
This section describes the EU Directives that apply to SE 250/260 Mighty Small II.
Conformity with EU Directives
This product fulfills the European Directives listed below. See the EU Declaration of
Conformity for the directives and regulations that apply for the CE marking.
If not included with the product, a copy of the EU Declaration of Conformity is available
on request.
TitleDirective
Low Voltage Directive (LVD)2014/35/EU
Restriction of Hazardous Substances (RoHS) Directive2011/65/EU
CE marking
The CE marking and the corresponding EU Declaration of Conformity is valid for the instrument when it is:
•
used according to the Operating Instructions or user manuals, and
•
used in the same state as it was delivered from GE, except for alterations described
in the Operating Instructions or user manuals.
8 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
1 Introduction
1.3 Regulatory information
1.3.1 EU directives
1.3.2 Eurasian Customs Union
Introduction
This section contains additional regulatory information to comply with the Eurasian
Customs Union technical regulations.
Manufacturer and importer
information
The table below summarizes the manufacturer and importer information required by
the Eurasian Customs Union.
InformationRequirement
See Manufacturing informationName and address of manufacturer
Telephone: + 46 771 400 600Telephone number of manufacturer
GE Healthcare LLCImporter and/orcompany forobtain-
ing information about importer
GE Healthcare Life Sciences
Presnenskaya nab., 10C, 12th floor
RU-123 317 Moscow, Russian Federation
Telephone 1: + 7 495 411 9714
Fax nr: + 7 495 739 6932
Email: LSrus@ge.com
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 9
1 Introduction
1.3 Regulatory information
1.3.2 Eurasian Customs Union
1.3.3 Regulations for USA and Canada
Introduction
This section describes the regulations that apply to SE 250/260 Mighty Small II in the
USA and Canada.
NRTL certification
This symbol indicates that the product has been certified by Intertek, which is a US Occupational Safety and Health Administration Nationally Recognized Testing Laboratory
(NRTL).
10 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
1 Introduction
1.3 Regulatory information
1.3.3 Regulations for USA and Canada
1.3.4 Other regulations and standards
Introduction
This section describes the standards that apply to the SE 250/260 Mighty Small II.
Environmental conformity
This product conforms to the following environmental requirements.
TitleRequirement
Waste Electrical and Electronic Equipment (WEEE) Directive2012/19/EU
Management Methods for the Restriction of the Use of Hazardous Substances in Electrical and Electronic Products.
China RoHS
Standards, applied to this
product
Standard requirements fulfilled by this product are summarized in the table below.
DescriptionStandard
Safety requirements for electrical equipment for measurement, control, and laboratory use - Part 1: General
requirements.
IEC/EN 61010-1, UL
61010-1, CAN/CSA-C22.2
No. 61010-1
Technical documentation forthe assessmentof electrical
and electronic products with respect to the restriction
of hazardous substances
EN 50581
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 11
1 Introduction
1.3 Regulatory information
1.3.4 Other regulations and standards
2 Safety information
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition,the chapterdescribes emergency and recovery procedures, and
provides recycling information.
Important
WARNING
Before installing, operating or maintaining the product, all users
must read and understand the entire contents of this chapter
to become aware of the hazards involved.
In this chapter
See pageSection
132.1 Safety precautions
202.2 Labels
222.3 Emergency procedures
232.4 Recycling information
242.5 Declaration of Hazardous Substances (DoHS)
12 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.1 Safety precautions
Introduction
The SE 250/260 Mighty SmallII ispowered by anexternal power supply. Before installing,
operating or maintaining the system, you must be aware of the hazards described in
this manual.
Follow the instructions provided to avoid injury to the operator or other personnel,
to the product, or to other equipment in the area.
The safety precautions in this section are grouped into the following categories:
•
General precautions
•
Personal protection
•
Using flammable liquids
•
Installing and moving the product
•
Power supply
•
System operation
•
Maintenance
Always follow the instructions below to avoid injury when using the SE 250/260 Mighty
Small II.
General precautions
WARNING
Before installing, operating or maintaining the product, all users
must read and understand the entire contents of this chapter
to become aware of the hazards involved.
WARNING
Only properly trained personnel may operate and maintain the
product.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 13
2 Safety information
2.1 Safety precautions
WARNING
Do not operate the SE 250/260 Mighty Small II in any other way
than describedin theSE 250/260Mighty SmallII OperatingInstructions.
WARNING
Do notdamage thepower supply cord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local GE
representative for replacements.
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
WARNING
Any liquid on the equipment must be dried off before connecting
the power supply.
CAUTION
Circulate only water or 50/50 water/ethylene glycol through the
heat exchanger. Never use anti-freeze or any organic solvent in
the heat exchanger.
CAUTION
Never introduce anti-freeze or any organic solvent into any part
of the instrument. Organic solvents will cause irreparable damage
to the instrument.
14 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.1 Safety precautions
Personal protection
WARNING
Always use appropriate Personal ProtectiveEquipment (PPE)during
operation and maintenance of this product.
WARNING
Hazardous substances and biological agents. When using haz-
ardous chemical and biological agents, take all suitable protective
measures, such as wearing protective clothing,glasses and gloves
resistant to the substances used. Follow local and/or national
regulations for safe operation and maintenance of this product .
WARNING
Spread of biological agents. The operator must take all necessary
actions toavoid spreading hazardous biological agents.The facility
must comply with the national code of practice for biosafety.
CAUTION
Handle theglass components with care! Wearappropriate personal
protective equipment (PPE).
Using flammable liquids
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 15
2 Safety information
2.1 Safety precautions
Installing and moving the
product
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
CAUTION
Turn off the power switch and remove connecting cables before
moving the equipment.
CAUTION
The electrophoresis unitis heavy,especially when filled with buffer.
Handle the unit with care to avoid personal injury.
Power supply
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by GE.
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
16 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.1 Safety precautions
WARNING
Make sure that there isaccess tothe instrument power supplycord
at all times.
Operation
WARNING
The highvoltage power supply must always be disconnectedwhen
the safety lid of the electrophoresis unit is taken off. The high voltage power supply must never be switched on unless the safety lid
is on the electrophoresis unit.
WARNING
Acrylamide is a neurotoxin. Always wear gloves and observe all
laboratory safety procedures.
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the productoutside these limits can
damage equipment and cause personal injury or death.
WARNING
Turn all power supply controls off and disconnect the power leads
before removing the safety lid.
WARNING
Always disconnect the high voltage leads from the power supply
before removing the lid from the unit.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 17
2 Safety information
2.1 Safety precautions
CAUTION
Circulate coolant throughthe heat exchanger to minimizeheating.
Overheating will cause irreparable damage to the unit! Do not
connect the heat exchanger to a water tap or any coolant source
where the water pressure is unregulated.
CAUTION
Do not operate with buffer temperatures above the maximum
specified technicalspecifications. Overheating will cause irreparable
damage to the unit!
CAUTION
Devices must only be operatedwith acertified power supply capable of limiting the voltage and current to specified ratings.
NOTICE
After initial monitoring, do not leave the unit unattended for more
than 45 min before checking the progress of the bands and the
buffer level.
NOTICE
If running only one gel, block off the unused part of the core with
a glass plate. Do not fill this side with buffer.
Maintenance
WARNING
Decontaminate before maintenance. To avoid personnel being
exposed to potentially hazardous substances, make sure that the
SE 250/260 Mighty Small II is properly decontaminated and sanitized before maintenance or service.
18 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.1 Safety precautions
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by GE may be used for maintaining or
servicing the product.
WARNING
Disconnect power. Always disconnect power from the instrument
before performing any maintenance task.
WARNING
Decommissioning. Decontaminatethe equipment before decom-
missioning to make sure that hazardous residues are removed.
NOTICE
Cleaning. Keep the exterior of the instrument dry and clean. Wipe
regularly with a soft damp tissue and, if necessary, a mild cleaning
agent. Let the instrument dry completely before use.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 19
2 Safety information
2.1 Safety precautions
2.2 Labels
Introduction
This section describes the system label and other safety or regulatory labels that are
attached to the product.
Description of symbols on the
system label
The table below describes the various symbols that may be found on the system label.
MeaningLabel
Warning! Read the user documentation before using the system.
Do not open any covers or replace parts unless specifically stated
in the user documentation.
This symbol indicates that the waste of electrical and electronic
equipment must not be disposed as unsorted municipal waste and
must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment.
This symbol indicates that the product does not contain toxic or
hazardous materials in excess of the limits established by the Chinese standard GB/T 26572 Requirements of concentration limits for
certain hazardous substances in electrical and electronic products,
and can be recycled after being discarded, andshould not be casually discarded.
The system complies with applicable European directives.
Eurasian Conformity mark: the single conformity mark indicates
that the product is approved for circulation on the markets of the
member states of the Eurasian Customs Union.
20 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.2 Labels
MeaningLabel
This symbolindicates thatthe product hasbeen certified by Intertek,
which is a US Occupational Safety and Health Administration Nationally Recognized Testing Laboratory (NRTL).
Serial number of the productSerial no.:
Year (YYYY) and month (MM) of manufactureManufac-
tured:
Safety labels
The table below describes the various symbols that may be found on the product.
DescriptionSymbol/text
Warning! Read the user documentation before using
the system. Do not open any covers or replace parts
unless specifically stated in the user documentation.
Warning! High Voltage. Always make sure that the
system is disconnected from electric power before removing the lid.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 21
2 Safety information
2.2 Labels
2.3 Emergency procedures
Introduction
This section describes how to shut down of the SE 250/260 Mighty Small II in an emergency situation, and the procedure for restarting the SE 250/260 Mighty Small II.
The section also describes the result in the event of power failure.
Precautions
WARNING
Make sure that there isaccess tothe instrument power supplycord
at all times.
Emergency shutdown
In anemergency situation, shut down the power supply in accordancewith itsemergency
procedure.
Power failure
In case of power failure to the SE 250/260 Mighty Small II, the run is interrupted immediately.
Restart after emergency
shutdown or power failure
To restart the run after an emergency shutdown or power failure, follow these steps:
ActionStep
Make sure all connections are in place.1
Start the power supply as described in the power supply's User Manual.2
22 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.3 Emergency procedures
2.4 Recycling information
Introduction
This section contains information about the decommissioning of the product.
Decontamination
The product must be decontaminated before decommissioning. All local regulations
must be followed with regard to scrapping of the equipment.
Disposal of the product
When taking the product out of service, the different materials must be separated and
recycled according to national and local environmental regulations.
Disposal of electrical
components
Waste electricaland electronic equipment mustnot bedisposed ofas unsortedmunicipal
waste and must be collected separately. Please contact an authorized representative
of the manufacturerfor information concerningthe decommissioning ofthe equipment.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 23
2 Safety information
2.4 Recycling information
2.5 Declaration of Hazardous Substances (DoHS)
根据SJ/T11364-2014《电子电气产品有害物质限制使用标识要求》特提供如下有关污染控制方面的
信息。
The following product pollution control information is provided according to SJ/T11364-2014 Marking
for Restriction of Hazardous Substances caused by electrical and electronic products.
电子信息产品污染控制标志说明
Explanation of Pollution Control Label
该标志表明本产品不含有超过中国标准 GB/T 26572 《电子信息产品中有毒有害物
质的限量要求》中限量的有毒有害物质,报废后可以进行回收处理,不能随意丢弃。
This symbol indicates that this electrical and electronic product does not contain any
hazardous substances above the maximum concentration value established by the
Chinese standard GB/T26572 , and can berecycledafter being discarded,and should
not be casually discarded.
24 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
2 Safety information
2.5 Declaration of Hazardous Substances (DoHS)
有害物质的名称及含量
Name and Concentration of Hazardous Substances
产品中有害物质的名称及含量
Table of Hazardous Substances’ Name and Concentration
有害物质
Hazardous substance
部件名称
Component name
多溴二苯醚
(PBDE)
多溴联苯
(PBB)
六价铬
(Cr(VI))
镉
(Cd)
汞
(Hg)
铅
(Pb)
OOOOOO80614745
OOOOOO80614935
本表格依据SJ/T 11364的规定编制。
This table is prepared according to SJ/T 11364.
0:
表示该有害物质在该部件所有均质材料中的含量均在 GB/T 26572规定的限量要求以下。
X:
表示该有害物质至少在该部件的某一均质材料中的含量超出 GB/T 26572规定的限量要
求。
•
此表所列数据为发布时所能获得的最佳信息.
0:
Indicates that this hazardous substance contained in all of the homogeneous materials for
this part is below the limit requirement in GB/T 26572.
X:
Indicates that this hazardous substance contained in at least one of the homogeneous
materials used for this part is above the limit requirement in GB/T 26572.
•
Data listed in the table represents best information available at the time of publication.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 25
2 Safety information
2.5 Declaration of Hazardous Substances (DoHS)
3 System description
About this chapter
This chapter gives an overview of the SE 250/260 Mighty Small II.
Illustration of the instrument
The illustration below shows the SE 250/260 Mighty Small II instrument.
1
2
3
4
5
6
8
7
FunctionPart
Color-coded leads (2)1
26 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
3 System description
FunctionPart
Safety lid2
Upper buffer chamber core3
Foam gasket4
Coolant port (2)5
Spring clamps (4)6
Positioning tabs7
SE 250/260 Mighty Small II Lower buffer chamber8
The following parts are included, but not shown in the illustration:
•
Glass plates
•
Notched alumina plates
•
Gel seal, 1/4 oz.
•
Spacer-Mate
•
Well-locating decal
Power supply with a minimum rating of 250 V, 50 mA, constant current or
constant voltage is required, but not included.
Note:
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 27
3 System description
4 Installation
About this chapter
This chapter provides required information to enable users and service personnel to
unpack the SE 250/260 Mighty Small II instrument.
Safety precautions
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
Unpacking procedure
Unwrap all packages carefully.
Inspect all visible parts for damage or missing pieces. If any damage is observed, record
this on the receiving documents and inform your GE representative. Make sure to keep
all packing material for damage claims or to use should it become necessary to return
the unit.
28 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
4 Installation
5 Operation
About this chapter
This chapter gives instructions on how to operate the product in a safe way.
Safety precautions
WARNING
The highvoltage power supply must always be disconnectedwhen
the safety lid of the electrophoresis unit is taken off. The high voltage power supply must never be switched on unless the safety lid
is on the electrophoresis unit.
WARNING
Acrylamide is a neurotoxin. Always wear gloves and observe all
laboratory safety procedures.
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the productoutside these limits can
damage equipment and cause personal injury or death.
CAUTION
Do not operate with buffer temperatures above the maximum
specified technicalspecifications. Overheating will cause irreparable
damage to the unit!
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 29
5 Operation
CAUTION
Circulate only water or 50/50 water/ethylene glycol through the
heat exchanger. Never use anti-freeze or any organic solvent in
the heat exchanger.
CAUTION
Devices must only be operatedwith acertified power supply capable of limiting the voltage and current to specified ratings.
NOTICE
After initial monitoring, do not leave the unit unattended for more
than 45 min before checking the progress of the bands and the
buffer level.
NOTICE
If running only one gel, block off the unused part of the core with
a glass plate. Do not fill this side with buffer.
In this chapter
See pageSection
315.1 Preparations
345.2 Assembly
405.3 Electrophoresis run
30 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
5 Operation
5.1 Preparations
Prepare the gel sandwich
Both precast and self-cast gels can be run in the SE 250/260 Mighty Small II units. This
unit accepts gels in 10 × 8 cm plates, which can be cast in SE215, SE245, or SE275 gel
casters.
Each unit includes notched alumina plates and rectangular glass plates. If casting your
own polyacrylamide gels, we recommend using a notched alumina ceramic back plate
because it transfers heat 40 times more rapidly than glass. For applications that are not
heat sensitive, a notched glass plate is available.
Before installingthe gels into the electrophoresis unit, the separating gel should already
be completely polymerized. Clean away any gel adhering to the notched alumina back
plate. The stacking gel (if applicable) can be cast in place on the electrophoresis unit.
Load liquid samples after the gel sandwich is installed.
Maintenance before use
Rinse the instrument before each use. Before using the first time, disassemble the unit
completely and wash with a dilute solution of a laboratory detergent and thoroughly
rinse with water and distilled water.
Check the gasket. Periodically remove the gray silicone rubber gasket from the core. Inspect for nicks and wear. If the gasket is intact, apply a light film of Gel seal, and replace
it inthe groove.Avoid stretching thegasket. Lay it onto the groove and pressit into place.
Disassemble a fully assembled
unit
ActionStep
Remove the safety lid by pressing on the handle at the top of the upper
buffer chamber core while lifting the lid by the bottom edges.
1
Empty all buffer chambers and remove any gel sandwiches.2
Depress both release tabs and lift out the upper buffer chamber core.3
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 31
5 Operation
5.1 Preparations
Optional cooling
Circulating pressure must not exceed 0.8 bar (12 psi) above ambient pressure. Do not
connect the cooling core to an unregulated coolant source such as a water tap.
To connect the cooling core to a circulator bath, follow these steps:
ActionStep
Slide hose clamps (4 in total) onto each end of two lengths of 8 mm (5/16")
vinyl or silicone tubing.
1
Attach one end of each length of tubing to a cooling core port.2
Attach the free ends of each length of tubing to the circulator bath ports;
one to the inlet and the other to the outlet.
3
Secure the connections with the hose clamps.4
Install the upper buffer chamber
core
CAUTION
Circulate only water or 50/50 water/ethylene glycol through the
heat exchanger. Never use anti-freeze or any organic solvent in
the heat exchanger.
ActionStep
Steady the lower chamber with one hand and then hold the core with the
other hand.
1
Position the lower chamber on the positioning tabs.2
Press down, listening for the core to snap into place. (Alternatively, depress
both release tabs at either side, position the core on the positioning tabs,
press into place, and release the tabs. Check that the core is secure.)
3
32 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
5 Operation
5.1 Preparations
ActionStep
To remove the core, depress both release tabs and lift.4
1
2
3
DescriptionPart
Handle1
Release tabs (2)2
Coolant port (2)3
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 33
5 Operation
5.1 Preparations
5.2 Assembly
Place the gel sandwich in the SE
250/260 Mighty Small II
WARNING
Acrylamide is a neurotoxin. Always wear gloves and observe all
laboratory safety procedures.
ActionStep
Rinse away the gel overlay with distilled water and drain any excess water.1
If installinga self-castor precast10 ×8 cm gel sandwich, orient the sandwich
so thatthe notchedplate facesthe gasket, notches at the top. Set the bottom
of thesandwich onthe supportingledges inthe bottomof thelower chamber
and center the plate so that the gasket seals both sides.
2
Place the gel sandwich in the SE
250/260 Mighty Small II
WARNING
Acrylamide is a neurotoxin. Always wear gloves and observe all
laboratory safety procedures.
34 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
5 Operation
5.2 Assembly
ActionStep
Rinse away the overlay with distilled water and drain any excess water.1
If installing a self-cast or precast 10 × 8 cm gel sandwich, align the bottom
of the plate with the bottom of the core (see figure below). The bottom of
the notched plate must cover the silicone rubber gasket. The 10 × 8 cm gel
sandwich fits flush with the bottom of the upper buffer chamber core.
2
If installing a self-cast or precast 10 × 10.5 cm gel sandwich, orient the
sandwich so that the notched plate faces the gasket, notches at the top.
Set the bottom of the sandwich on the supporting ledges in the bottom of
the lower chamber and center the plate so that the gasket seals both sides
(see figure below). The 10 × 10.5 cm gel sandwich fits against the bottom of
the lower buffer chamber.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 35
5 Operation
5.2 Assembly
Clamp the sandwich in place
Cooling is optional. If desired, attach tubing to ports on both sides of the core
before attaching gel sandwiches. Circulate coolant. See Optional cooling, on
page 32.
Note:
ActionStep
Lightly press the sandwich against the gasket and secure it to the core with
one spring clamp on each side.
1
Position the jaw so that the shorter rounded jaw edge fits into the core
groove and the longer edge sits on the glass plate.
2
Slide the clamps down to the stop.
Note:
Proper positioning is important to achieve a seal and to minimize glass
breakage.
3
Repeat step 1-3 for the second sandwich, or, if running only one gel, clamp
a plain glass plate on the unused side of the core to prevent a possible short
circuit with the unused electrode. Do not fill this chamber with buffer if no
gel sandwich is in place.
4
Sample preparation and loading
If applicable, cast the stacking gel in the unit.
Calculate the stacking gel monomer solution volume:measure the distance, in cm, from
the top of the resolving gel to the notch in the alumina plate. (This should be at least 2
cm—more if the sample depth in the well is unusually high.) Multiply this distance by the
gel width (8.3 cm) and the gel thickness (cm). This product is the required volume in ml.
If wells are already in place, start at with preparing the sample.
36 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
5 Operation
5.2 Assembly
To prepare the wells, follow these steps:
ActionStep
De-aerate the stacking gel monomer solution.1
Add catalyst and initiator to the stacking gel monomer solution and then
pour.
2
Use a pipette to deliver the solution into one corner of the plate, taking care
not to trap any bubbles.
3
Insert a comb (at a slight angle to prevent trapping air) into the sandwich,
allowing the comb sides to rest on the spacers.
4
Overlay each gel with a thin layer of water-saturated n-butanol, water, or
diluted gel buffer to prevent gel exposure to oxygen. Slowly deliver the
overlay solution from a glass syringe fitted with a 22-gauge needle. Apply
the solution near the spacer at the side of the sandwich and allow it to flow
across the surface unaided.
5
Allow a minimum of one hour for the gel to polymerize.6
To prepare the sample, follow these steps:
ActionStep
Increase liquid sample density with 10% glycerol or sucrose.1
Add a tracking dye.2
For SDS protein gels, use 2× treatment buffer to denature both liquid and
dry samples in a test tube.
3
To liquid protein solutions, add an equal volume of 2× buffer.
To dry protein samples, add equal volumes of buffer and ddH2O to achieve
the desired concentration.
Heat the tube in boiling water for 90 seconds, then chill it in ice until ready
to use.
4
Treated samples can be stored at -40°C to -80°C for future runs.
To load samples, follow these steps:
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 37
5 Operation
5.2 Assembly
ActionStep
To aid in loading samples, wet the well-locating decal and apply it to the
front of the glass plate so that the appropriate edge outlines the sample
wells.
Note:
The side wells for standards of a preparative comb correspond to the outermost wells formed by the 10-well comb.
1
Fill the sample wells and each upper buffer chamber that will be used with
running buffer. One upper buffer chamber holds approximately 75 mL.
2
Load the sample into the wells using a fine-tipped microsyringe. The width
of the wells depends on the number of wells per comb.For the volume required to fill to 1mm well depth, see the table below.
3
Volume of sample (µL) per 1 mm depth
Comb thickness (mm)No. of wells
1.51.00.75
19.112.79.55
5.89
7.24.83.610
4.42.92.215
2.918
Final assembly
CAUTION
Devices must only be operatedwith acertified power supply capable of limiting the voltage and current to specified ratings.
38 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
5 Operation
5.2 Assembly
ActionStep
Fill the lower buffer chamber with running buffer.1
The SE 250/260 Mighty Small II holds about 250 mL. Make sure that the
lower electrode (running along the bottom of the the upper buffer chamber
core) is completely submerged.
Note:
If using precast gels, check that the lower gel/buffer contact surface is exposed
(the colored plastic tape must be removed).
Place the safety lid on the unit.2
Plug the color-coded leads into the jacks of an approved power supply. The
red lead plugs into the red output jack, and the black lead plugs into the
black output jack.
3
Optional cooling:Begin circulating coldwater ora chilled 50/50 water/ethylene glycol solution.
4
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 39
5 Operation
5.2 Assembly
5.3 Electrophoresis run
Running the gel
Gels maybe run at either constant currentor constantvoltage. Aconstant current setting
is traditionallyused witha discontinuous buffer system so that the rate of electrophoretic
migration remains unchanged throughout the run. Under these conditions, voltage increases as the run proceeds. A lower current setting is recommended for higher resolution. Precast gels are run under the same current and voltage conditions as self-cast
gels.
It takes about one hour to run two 7 cm × 0.75 mm Laemmli gels at 40 mA (20 mA per
gel, constant current). Check band progress after 5 minutes, and again after half an
hour, keeping an eye on the position of the tracking dye. The run is complete when the
tracking dye reaches the bottom of the gel. Watch the buffer level in the upper buffer
chamber and, if necessary, replenishit before itfalls below the level of the notchedplate.
A small volume of buffer may leak past a chipped plate or nicked gasket, or it may wick
out through the gel.
NOTICE
After initial monitoring, do not leave the unit unattended for more
than 45 min before checking the progress of the bands and the
buffer level.
After the run
WARNING
Always disconnect the high voltage leads from the power supply
before removing the lid from the unit.
ActionStep
Once the tracking dye reaches the bottom of the gel, turn off the power
supply, disconnect the leads, and remove the safety lid as described in Dis-
assemble a fully assembled unit, on page 31
1
If coolant is circulating, stop the flow and disconnect the fittings or tubing.2
40 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
5 Operation
5.3 Electrophoresis run
ActionStep
Remove the core assembly with gels attached by squeezing the release tabs
and lift out the core assembly.
3
Pour outthe bufferby inverting the core assembly,then remove bothclamps,
and lift away gel sandwich(es) from the upper buffer chamber core.
4
Gently loosen and then slide away both spacers.5
Slip an extra spacer or a Wonder Wedge into the bottom edge (to prevent
breaking the ears of the notched plates) and separate the plates. The gel
usually adheres to the alumina plate.
6
Carefully lift the gel from the plate and lay it into a tray of stain or fixative.7
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 41
5 Operation
5.3 Electrophoresis run
6 Maintenance
About this chapter
This chapter provides information to enable users and service personnel to clean and
maintain the product.
Precautions
WARNING
Decontaminate before maintenance. To avoid personnel being
exposed to potentially hazardous substances, make sure that the
SE 250/260 Mighty Small II is properly decontaminated and sanitized before maintenance or service.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by GE may be used for maintaining or
servicing the product.
WARNING
Disconnect power. Always disconnect power from the instrument
before performing any maintenance task.
General procedures
Immediately after each use, rinse the unit with water and then rinse thoroughly with
distilled water. Do not use organic solvents, abrasives, strong cleaning solutions, or
strong acids or bases to clean the chambers. Handle the upper buffer chamber core
with care to prevent damage to the banana plugs. Allow to air dry.
Clean glassand aluminaplates andspacers witha dilutesolution ofa laboratory cleanser,
then rinse thoroughly with tap and distilled water. Glass plates can also be treated with
(but notstored in) acid cleaning solutions. Do not autoclave or heat any part above 45°C.
42 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
6 Maintenance
Cleaning before planned
maintenance/service
To ensure the protection and safety of service personnel, all equipment and work areas
must be clean and free of any hazardous contaminants before a Service Engineerstarts
maintenance work.
Please complete the checklist in the On Site Service Health and Safety Declaration Form
or the Health and Safety Declaration Form for Product Return or Servicing, depending on
whether theinstrument isgoing to be serviced on site or returned for service, respectively.
Copy theform you needfrom Section 8.3 Health and Safety Declaration Forms, on page 50.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 43
6 Maintenance
7 Troubleshooting
About this chapter
This chapter provides information to assist users and service personnel to identify and
correct problems that may occur when operating the product.
If the suggested actions in this guide do not solve the problem, or if the problem is not
covered by this guide, contact your GE representative for advice.
Safety precautions
CAUTION
Circulate only water or 50/50 water/ethylene glycol through the
heat exchanger. Never use anti-freeze or any organic solvent in
the heat exchanger.
CAUTION
Never introduce anti-freeze or any organic solvent into any part
of the instrument. Organic solvents will cause irreparable damage
to the instrument.
Potential problems
Corrective actionError description
To reduce the running temperature:Smile effect on the
buffer front
•
Circulate coolant throughthe upper buffer chamber core.
•
Prechill the buffer.
•
Decrease the current or voltage setting. (10 mA per 0.75
mm gel, 15 mA per 1.5 mm thick gel.)
•
Run the gel in the cold room.
44 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
7 Troubleshooting
Corrective actionError description
Centrifuge or filter sample before loading to remove particulates.
Protein streaks vertically
Dialyze or desalt the sample.
To increase ordecrease the migrationrate, adjust the voltage
or current by 25% to 50%.
Unusually slow (or
fast) run
Adjust the solutions:
•
Check recipes, gelconcentrations, solutions,and dilutions.
(For instance, do not use Tris-HCl instead of Tris.)
•
If the required pH of a solution is exceeded, do not backtitrate. Prepare a fresh buffer.
•
Dispose of older acrylamide solutions and use only stock
of the highest quality.
•
Only use freshly deionized urea.
Check gel preparation and polymerization.Bands are skewed
or distorted
De-gas the stacking gel solution and avoid trapping air bubbles under the comb teeth.
Overlay therunning gelwith water-saturated n-butanolbefore
polymerization begins to avoid forming an uneven gel surface.
Check sample preparation.
Dialyze or desalt the sample.
Centrifuge or filter sample before loading to remove particulates.
Near the buffer front:Stained sample col-
lects
•
Protein is not sufficiently restricted by the resolving gel;
increase the % T.
Near the top of the gel when the buffer front has reached the
bottom:
•
The gel pore size is too small. Decrease the % T of the resolving gel.
•
The protein has precipitated. Heat the sample at a lower
temperature (70°C or less) for 1–2 minutes.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 45
7 Troubleshooting
Corrective actionError description
Use only the highest quality reagents.Poor band resolu-
tion
Conduct the separation at a lower current or voltage setting.
Dialyze or desalt the sample.
Reduce the sample volume or concentration.
Only use freshly deionized urea.
Improve dissociation of subunits by heating sample in SDS
sample buffer 1–2 minutes at 100°C.
Add more mercaptoethanol or dithiothreitol; check sample
treatment.
Only use gels that were recently prepared.
Check pHvalues of the separating and stacking gel solutions.
Do not back-titrate buffers.
Sample preparation:
•
Heat samples for no more than 1–2 minutes at 100°C.
Store on ice after heating.
•
Store sample on ice before it is denatured.
•
Add protease inhibitorsif necessary to preventproteolytic
degradation of sample.
•
Store samples to be frozen in aliquots to prevent repeated
freezing and thawing. (Store at -40% to -80%.)
Pour a taller stacking gel. (For best results, allow a stacking
gel height of 2.5 times the height of the sample in the well.)
Bromophenol blue
does not sharpen
into a concentrated
zone in the stacking
gel
Dispose of outdated acrylamide solutions and use only the
highest grade of acrylamide.
When preparing samples, avoid using solutions with a high
sodium or potassium concentration.
46 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
7 Troubleshooting
8 Reference information
About this chapter
This chapter lists the technical specifications of the SE 250/260 Mighty Small II. The
chapter also includes ordering information, and the Health and Safety Declaration form
for service.
In this chapter
See pageSection
488.1 Specifications
498.2 Ordering information
508.3 Health and Safety Declaration Forms
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 47
8 Reference information
8.1 Specifications
SpecificationParameter
10 × 8 cmGel plate size
8 × 7 cmApproximate gel size
12 WMaximum power
500 VMaximum voltage
500 mAMaximum current
45°CMaximum gel temperature
Indoor use: 4°C to 40°CEnvironmental operating
conditions
Humidity up to 80%
Altitude up to 2000 m
IIInstallation category
2Pollution degree
16.5 × 16 × 16 cmDimensions (W × H × D)
(6.5 × 6.3 × 6.3 in.)
2.7 kgWeight
48 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
8 Reference information
8.1 Specifications
8.2 Ordering information
For product codes and information about how to order, please see
www.gelifesciences.com
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 49
8 Reference information
8.2 Ordering information
8.3 Health and Safety Declaration Forms
On site service
On Site Service Health &
Safety Declaration Form
Service Ticket #:
To make the mutual protection and safety of GE service personnel and our customers, all equipment and work areas must be
clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your
equipment, please complete this checklist and present it to the Service Engineer upon arrival. Equipment and/or work areas
not sufficiently cleaned, accessible and safe for an engineer may lead to delays in servicing the equipment and could be subject to
additional charges.
Yes
No
Please review the actions below and answer “Yes” or “No”.
Provide explanation f or any “No” answers in box below.
Instrument has been cleaned of hazardous substances.
Please rinse tubing or piping, wipe down scanner surfaces, or otherwise ensure removal of any dangerous
residue. Ensure the area around the instrument is clean. If radioactivity has been used, please perform a
wipe test or other suitable survey.
Adequate space and clearance is provided to allow safe access for instrument service, repair or
installation. In some cases this may require customer to move equipment from normal operating location
prior to GE arrival.
Consumables, such as columns or gels, have been removed or isolated from the instrument and from
any area that may impede access to the instrument .
All buffer / waste vessels are labeled.
Excess containers have been removed from the area to provide access.
Provide
explanation
for any “No”
answers here:
Equipment type / Product No:
Serial No:
I hereby confirm that the equipment specified above has been cleaned to remove any hazardous substances and that the
area has been made safe and accessible.
Name:
Company or institution:
Position or
job title:
Date (YYYY/MM/DD):
Signed:
GE and GE monogram are trademarks of General El ectri c Company.
GE Healt hcare Bio-Scien ces Corp, 800 Centennial Avenue, P.O. Box 1327, Piscataway,
NJ 08855-1327
© 2010-14 General Electric Company—All rights reserved. First pub lis hed April 2010.
DOC114954 2/28-9800-26 AC 05/201 4
50 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
8 Reference information
8.3 Health and Safety Declaration Forms
Product return or servicing
Health & Safety Declaration
Form
for Product Return or
Servicing
Return authorization
number:
and/or
Service Ticket/Request:
To make sure the mutual protection and safety of GE personnel, our customers, transportation personnel and our environment,
all equipment must be clean and free of any hazardous contaminants before shipping to GE. To avoid delays in the processing of
your equipment, please complete this checklist and include it with your return.
1. Please note that items will NOT be accepted for servicing or return without this form
2. Equipment which is not sufficiently cleaned prior to return to GE may lead to delays in servicing the equipment and
could be subject to additional charges
3. Visible contamination will be assumed hazardous and additional cleaning and decontamination charges will be applied
Yes
No
Please specify if the equipment has been in contact with any of the following:
Radioactivity (please specify)
Infectious or hazardous biological substances (please specify)
Other Hazar
dous
Chemicals (please specify)
Equipment must be decontaminated prior to service / return. Please provide a telephone number where G E can
contact
you for additional information concerning the system / equipment.
Telephone No:
Liquid and/or gas in equipment is:
Water
Ethanol
None, empty
Argon, Helium, Nitrogen
Liquid Nitrogen
Other, please
specify
Equipment type / Product No:
Serial No:
I hereby confirm that the equipment specified above has been cleaned to remove any hazardous substances and that the
area has been made safe and accessible.
Name:
Company or
institution:
Position or job title:
Date (YYYY/MM/DD)
Signed:
To receive a return authorization num ber or service number,
please call local technical support or customer service.
GE and GE monogram are trademarks of General El ectri c Company.
GE Healt hcare Bio-Scien ces Corp, 800 Centennial Avenue, P.O. Box 1327, Piscataway,
NJ 08855-1327, US
© 2010-14 General Electric Company—All rights reserved. First pub lis hed April 2010.
DOC114954 4/28-9800-27 AC 05/201 4
SE 250/260 Mighty Small II Operating Instructions 29281629 AA 51
8 Reference information
8.3 Health and Safety Declaration Forms
Index
C
CE
conformity, 8
marking, 8
Cleaning, 42
Before service, 43
D
Decommisioning, 23
E
Emergency
procedures, 22
Emergency procedures
emergency shutdown, 22
power failure, 22
restart, 22
G
Gel
running, 40
Gel sandwich
assembly, 34, 36
preparation, 31
I
Important user information, 5
Intended use, 5
L
Labels, 20
Safety labels, 21
System label, 20
M
Maintenance, 42
Manufacturing information, 7
N
Notes and tips, 6
NRLT, 10
O
On site service, 50
Operation, 29
Ordering information, 49
P
Prerequisites, 5
Product return or servicing, 51
Purpose of this manual, 4
R
Recycling information
decontamination, 23
disposal ofelectrical components, 23
Regulatory information, 7, 10
S
Safety information, 12
Safety label
Description, 21
Safety notices, 6
Safety precautions
Installation, 16
introduction, 13
Maintenance, 18
Power supply, 16
System operation, 17
Samples
loading, 36
preparation, 36
SE 250/260 Mighty Small II
illustration, 26
main components, 26
Service
Cleaning before, 43
Specifications, 48
Standards, 11
System label
Description, 20
T
Troubleshooting, 44
U
Unpacking, 28
Unpacking procedure, 28
52 SE 250/260 Mighty Small II Operating Instructions 29281629 AA
Index
GE and the GE Monogram are trademarks of General Electric Company.
© 2004-2018 General Electric Company
All goods and services are sold subject to the terms and conditions of sale of
the company within GE Healthcare which supplies them. A copyof these terms
and conditions is available on request. Contact your local GEHealthcare representative for the most current information.
GE Healthcare Bio-Sciences AB
Björkgatan 30, 751 84 Uppsala, Sweden
GE Healthcare Europe GmbH
Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare Bio-Sciences Corp.
100 Results Way, Marlborough, MA 01752, USA
HyClone Laboratories, Inc.
925 W 1800 S, Logan, UT 84321, USA
GE Healthcare Japan Corporation
Sanken Bldg. 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073, Japan
For local office contact information, visit
www.gelifesciences.com/contact
GE Healthcare UK Limited
Amersham Place
Little Chalfont
Buckinghamshire, HP7 9NA
United Kingdom
www.gelifesciences.com
29281629 AA 03/2018 a155
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