GE Monitor UV-900 User Manual
ÄKTA
™
Monitor UV-900
User Manual
GE Healthcare
Important user information
All users must read this entire manual to fully under-
stand the safe use of Monitor UV-900.
WARNING !
The WARNING! sign hig hlights instructions
that must be followed to avoid personal
injury. It is important not to proceed until
all stated conditions are met and clearly
understood.
CAUTION!
The Caution! sign highlights instructions that must be
followed to avoid damage to the product or other equip-
ment. It is important not to proceed until all sta ted con-
ditions are met and clearly understood.
Note
The Note sign is used to indicate information important
for trouble-free and optimal use of the product.
CE Certifying
This product meets the requir e ment s of app licable CE-
directives. A copy of the corresponding Declaration of
Conformity is available on request.
The CE symbol and corre spon ding dec lara tion of con for-
mity, is valid for the instrument when it is:
– used as a stand-alone unit, or
– connected to other CE-marked GE Healthcare
instruments, or
– connected to other products recommended or
described in this manual, and
– used in the same state as it was delivered from GE
Healthcare except for alterations described in this
manual.
Recycling
This symbol indicates that the waste of
electrical and electronic equipment must
not be disposed as unsorted municipal
waste and must be collected separately.
Please contact an au thorized r epr ese nta-
tive of the manufacturer for information
concerning the decommissioning of equip-
ment.
WARNING!
This is a Class A pruduct. In a domestic environment this
product may cause radio interference in whi ch ca se the
user may be required to make adequate measures
Monitor UV-900 User Manual 18-1120-05 Edition AF
Monitor UV-900 User Manual 18-1120-05 Edition AF
V
Contents
1 Introduction
1.1 General ................................................................................................7
1.2 Safety ...................................................................................................8
2 Installation
2.1 Unpacking ..........................................................................................9
2.2 General precautions .....................................................................9
2.3 Installing the flow cell ................................................................10
2.3.1 Fixing the flow cell.................................................................... 10
2.3.2 Connecting the optical fibers...............................................11
2.4 Connecting electrical signal cables .................................... 12
2.4.1 Connecting to recorder (if used)..........................................12
2.5 Connecting to communication link .....................................12
2.6 Connecting to supply voltage ...............................................13
2.7 Connecting the tubing .............................................................. 13
2.8 Mounting the cell holder cover .............................................14
3Operation
3.1 On/off ................................................................................................15
3.2 Menu selection and settings ..................................................15
3.2.1 Menu selection ...........................................................................15
3.2.2 Return to main menu .............................................................. 16
3.2.3 Select value..................................................................................16
3.3 Main menu overview .................................................................17
3.4 Setting lamp on/off .....................................................................17
3.5 Reading absorbance values ...................................................18
3.6 Setting wavelength .....................................................................18
3.7 Autozero .......................................................................................... 19
3.8 Storage and shut-down ...........................................................19
3.9 Using an external chart recorder ........................................20
3.9.1 Setting range and zero...........................................................20
3.9.2 Event mark...................................................................................20
3.10 Filtering noise ................................................................................21
3.11 UV cell calibration .......................................................................22
3.12 Changing flow cell .......................................................................24
3.13 Restart after power failure ...................................................... 24
4 Maintenance
4.1 Periodic maintenance ...............................................................25
Contents
VI
Monitor UV-900 User Manual 18-1120-05 Edition AF
4.2 Cleaning-in-place .........................................................................25
4.3 Checking the instrument ..........................................................26
4.3.1 Lamp intensity ............................................................................26
4.3.2 Lamp on–time .............................................................................26
4.3.3 Flip time..........................................................................................26
4.3.4 UV flow cell ...................................................................................26
4.4 Cleaning the flow cell and optical fiber connectors ....27
4.4.1 Cleaning the flow cell...............................................................27
4.4.2 Cleaning the optical fiber connectors...............................27
4.4.3 Instrument housing...................................................................27
4.5 ..................................................................Cleaning the system 27
4.6 Recycling ..........................................................................................28
5 Trouble-shooting
5.1 General .............................................................................................29
5.2 Faults and actions .......................................................................29
5.3 Error messages .............................................................................30
Reference information
A Description ........................................................................................................31
A.1
Instrument
............................................................................................................31
A.2
Flow cell
.................................................................................................................32
A.3
Monitor principle
................................................................................................32
B Menus .................................................................................................................. 34
B.1
Check menu
.......................... ..... ..... ..... ..... ..... ..... ..... ..... ..... .... ..... ..... ..... ..... ..... .....34
B.2
Setup menu
..........................................................................................................36
B.3
Setting and using the alarm timer
.............................................................37
B.4
Service displays
..................................................................................................37
B.5
Menu overview
...................................................................................................38
C Technical specifications .............................................................................39
D Accessories and spare parts ....................................................................42
Short instructions on side 39
About this manual
This manual comprises two parts; a practical part
(sections 1–5) and a reference part (sections A-D).
Sections 1–5 contain the necessary information for operating the
instrument.
Introduction 1
Monitor UV-900 User Manual 18-1120-05 Edition AF
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1 Introduction
1.1 General
Monitor UV-900 is a UV-absorption monitor for use in liquid chromatography.
Monitor UV-900 features:
• Variable wavelength for detection in the range 190–700 nm.
• Up to 3 wavelengths can be monitored simultaneously.
• Two alternative flow cells with path length 2 mm and 10 mm.
• Accurate and reliable monitoring through self-test, self-calibration and
fiber optics.
M
on
ito
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V
-9
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1 Introduction
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Monitor UV-900 User Manual 18-1120-05 Edition AF
1.2 Safety
• The instrument is designed for indoor use only.
• Do not use in a dusty atmosphere or close to spraying water.
IMPORTANT!
Monitor UV-900 is intended for laboratory use only, not for
clinical or in vitro use, or for diagnostic purposes.
WARNING!
When using hazardous chemicals, all suitable protective
measures, such as protective glasses, must be taken.
WARNING
! The instruments must not be opened by the user. They contain
high voltage circuits which can give a lethal electric shock.
WARNING
! The system must be connected to a grounded mains socket.
WARNING
! Always disconnect the power supply before attempting to
replace any item on the instrument.
WARNING!
The instrument uses high intensity ultra-violet light. Do not
disconnect the optical fibers while the monitor is running.
CAUTION!
The flow cell must not be used at pressures above 2 MP a (20 bar,
290 psi). At higher pressures the flow cell may break.
WARNING!
When using hazardous chemicals, take all suitable protective
measures, such as wearing protective glasses and gloves resistant to the
chemicals used. Follow local regulations and instructions for sa fe operati on
and maintenance of the system.
WARNING!
There must always be a sample loop connected to ports 2 and 6
of the injection valve. This is to prev ent liquid spraying out of the ports when
switching the valve. This is especially dangerous if hazardous chemicals are
used.
WARNING!
This is a Class A product. In domestic environment this product
may cause radio interference in which the user may be required to take
adequate measures.
Installation 2
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2 Installation
2.1 Unpacking
Unpack the instrument and check the items against the supplied packing list.
Inspect the items for obvious damage which may have occurred during
transportation.
• The system should be installed on a stable laboratory bench providing a
suitable working area.
• To maintain correct ventilation, the system requires an appropriate
amount of free space. Do not block the ventilation inlets or outlets on the
system!
It is recommended that all packing materials should be retained if onward
transport of the instrument is expected.
2.2 General precautions
The instrument should not be installed in a corrosive atmosphere or in an
atmosphere where deposits are liable to form on the optical surfaces.
The instrument should be located in a place of low temperature variations,
away from heat sources, draughts and direct sunlight.
The instrument may be operated at normal ambient temperatures in the range
+4 to +40 °C.
The instrument should be installed on a stable laboratory bench or in
ÄKTA™
explorer or ÄKTApurifier. To ensure correct ventilation a free space of
0.1 m is required behind and in front of the instrument. Place the instrument
directly on the bench. Do not use any soft material under the instrument, to
ensure that the ventilation inlet in the front is not blocked.
CAUTION! The following information should be read car efully to ensur e that
the instrument is installed correctly.
Monitor UV
-900
2 Installation
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Monitor UV-900 User Manual 18-1120-05 Edition AF
2.3 Installing the flow cell
2.3.1 Fixing the flow cell
Handle the optical fibers with care,
do not bend excessively.
Two flow cells are available: UV-900/2 with 2 mm light path length
(cell volume 2 µl) and UV-900/10 with 10 mm path length (8 µl).
1 Unpack the flow cell. Do not remove the r ed pr otective caps f r om the inle t
or outlet, or the black protective caps on the optical fiber connectors.
2 Slide the rear white clip on the cell holder to its inner position for a 2 mm
cell and to its outer position for the 10 mm cell.
3 Place the flow cell in the opening between the white clips. The cell should
be positioned with the text and serial number facing upwards and to the
front. Press the cell into the clips to fasten it.
10 mm
2 mm
CAUTION!
To avoid damaging the optical fibers, press only on the cell body,
never on the optical fibers.
Installation 2
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To improve t he access to the tubing connections, the flow cell can alternatively
be positioned with the tubing connections.
2.3.2 Connecting the optical fibers
1 Remove the 2 black protective caps from the optical fiber connectors.
2 Remove the 2 rubber protective caps from the optical fiber receptacles on
the right side of the housing.
3 Connect the 2 optical fibers to the housing by carefully inserting them into
the sockets and tightening the nut s fingertight using the fiber detachment
tool supplied.
4 Slide the rubber sleeves on the 2 optical fibers onto the connectors. Make
sure that the sleeves are pushed tight to the housing to prevent dust or
fluid entering the connection.
Serial number
Normal position
Serial number
Alternative position
CAUTION! Do not touch the tips of the optical fibers with your fingers as
this will result in poor monitor performance. If you accidentally touch the
optical fiber tip, it can be wiped with 30% isopropanol using lens pape
r.
Fiber
detachmen
t
tool
CAUTION! Do not twist the optical fibers during tightening.
Rubber sleeve
2 Installation
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Monitor UV-900 User Manual 18-1120-05 Edition AF
2.4 Connecting electrical signal cables
The sockets for electrical signals are located on the back panel.
2.4.1 Connecting to recorder (if used)
1 Connect the recorder to the miniDIN-socket
Analogue out using the cable
supplied. Each wavelength signal is available on a separate channel using
the following wires:
Note:
The signal cable is delivered with protective covers on each wir e. Do
not remove the protective covers from unused connections as a
short circuit may disturb the measurements.
2 Set the recorder to 0–1 V input, full scale, 0 V offset.
2.5 Connecting to communication link
The monitor can be used in ÄKTAexplorer or ÄKT Apurifier and controlled from a
PC running UNICORN™ version 3.21 or higher, using UniNet cables.
Analogue out
0-1V
Remote
UniNet 1 Mains On/off
λ
1 Wire 1 +
Wire 2 –
λ
2 Wire 3 +
Wire 4 -
λ
3 Wire 5 +
Wire 6 -
CAUTION!
The mains power to the ÄKTAexplorer or ÄKTApurifier must be
switched OFF before connecting the instrument to the UniNet-1 link.
WARNING!
Only use mains cables delivered or approved by GE Healthcare.
WARNING!
Do not block the rear panel of the system. The mains power
switch must always be easy to access.
Installation 2
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Connect two UniNet cables to the UniNet-1 connectors. The inst rument can be
connected in series anywhere in the chain between the PC and the termination
plug. The UniNet-1 link connects, in series, the PC with Pump P-905,
Monitor pH/C-900 and Monitor UV-900. The termination plug is connected to
the last instrument in the chain.
2.6 Connecting to supply voltage
1 Make sure the on/off switch is in the OFF position (O).
2 Connect a mains cable between the instrument and a grounded mains
socket. The instrument is delivered with both European and US type mains
cables, as standard. Any voltage 100–240 V AC, 50–60 Hz, can be used.
The instrument contains no user replaceable fuse.
2.7 Connecting the tubing
1 Remove the red protective caps from the inlet and outlet of the flow cell
and connect the tubing with 1/16" “fingertights”.
The inlet is on the top of the flow cell, the same side as the serial number.
WARNING!
The instrument must be connected to a grounded mains socket.
Inlet
Outlet
Installation 2
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2.8 Mounting the cell holder cover
The cover is a simple push fit onto the cell holder. Two small lugs on the cover
locate in holes at the front and rear of the cell holder The cover is then lowered
over the cell holder.
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