1036.2Preparing the sample .........................................................................................................................
1046.2.1Preparing a sample for fluorescence imaging ..................................................................
1096.2.2Preparing a sample for phosphor imaging ........................................................................
1166.2.3Preparing a sample for densitometry ...................................................................................
1176.3Performing a scan ................................................................................................................................
1226.4Procedures after a scan ....................................................................................................................
This chaptercontains importantuser information,descriptions ofsafety notices,regulatory information, and intended use of the Amersham Typhoon system.
In this chapter
This chapter contains the following sections:
See pageSection
51.1 About this manual
61.2 Important user information
81.3 Regulatory information
151.4 Abbreviations
4Amersham Typhoon Operating Instructions 29193226 AC
1.1About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions cover all configurations of Amersham Typhoon scanner and
the Amersham Typhoon Control Software. The illustration below shows the
Amersham Typhoon scanner.
1 Introduction
1.1 About this manual
Descriptions of Amersham Eraser and Amersham Cabinet are not covered in this document.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text (for example, Power switch).
All users must read the entire Operating Instructions before installing, operating or
maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use of the
Amersham Typhoon scanner
The Amersham Typhoon scanner is a versatile laser scanner that can be used for many
different imaging applications, including:
•
imaging of storage phosphor screens
•
fluorescence imaging with multiple lasers, for example for muliplex Western blotting
and two-dimensional difference gel electrophoresis (2D DIGE)
•
imaging of gels with colorimetric stains
•
densitometric imaging, for example of Coomassie™ blue stained gels
Amersham Typhoon scanner is intended for research use only, and shall not be used in
any clinical procedures or for diagnostic purposes.
Prerequisites
In order to operate Amersham Typhoon scanner in the way it is intended, the following
prerequisites must be fulfilled:
•
You must have read and understood the safety instructions in the user documentation.
•
All operations should be performed by qualified personnel who are adequately
trained.
6Amersham Typhoon Operating Instructions 29193226 AC
Safety notices
1 Introduction
1.2 Important user information
•
Amersham Typhoon scanner must be installed in accordance with the site requirements and instructions in Chapter 5 Installation, on page 81.
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could resultin deathor serious injury. It is important notto proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
A note is used to indicate information that is important for trouble-free and
optimal use of the product .
A tip contains useful information that can improve or optimize your procedures.
1 Introduction
1.3 Regulatory information
1.3Regulatory information
Introduction
This sectionlists theregulations and standards that applyto Amersham Typhoon scanner.
Manufacturing information
The table below summarizes the required manufacturing information.
In this section
InformationRequirement
GE Healthcare Bio-Sciences AB,Name and address of manufacturer
Björkgatan 30, SE 751 84 Uppsala, Sweden
See pageSection
91.3.1 EU Directives
101.3.2 Eurasian Customs Union
111.3.3 Regulations for USA and Canada
121.3.4 Korean regulatory information
131.3.5 Other regulations and standards
8Amersham Typhoon Operating Instructions 29193226 AC
1.3.1EU Directives
Conformity with EU Directives
This product fulfills the European Directives listed below. See the EU Declaration of
Conformity for the directives and regulations that apply for the CE marking.
If not included with the product, a copy of the EU Declaration of Conformity is available
on request.
This section contains additional regulatory information to comply with the Eurasian
Customs Union technical regulations.
Manufacturer and importer
information
The table below summarizes the manufacturer and importer information required by
the Eurasian Customs Union.
ing information about importer
InformationRequirement
See Manufacturing informationName and address of manufacturer
Telephone: + 46 771 400 600Telephone number of manufacturer
GE Healthcare LLCImporter and/orcompany forobtain-
GE Healthcare Life Sciences
Presnenskaya nab., 10C, 12th floor
RU-123 317 Moscow, Russian Federation
Telephone 1: + 7 495 411 9714
Fax nr: + 7 495 739 6932
Email: LSrus@ge.com
10Amersham Typhoon Operating Instructions 29193226 AC
1.3.3Regulations for USA and Canada
NRTL certification
This symbol indicates that Amersham Typhoon has been certified by a Nationally Recognized Testing Laboratory (NRTL).
NRTL means an organization, which is recognized by the US Occupational Safety and
Health Administration (OSHA) as meeting the legal requirements of Title 29 of the Code
of Federal Regulations (29 CFR), Part 1910.7.
FCC compliance
This device complies with part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) This device may not cause harmful interference, and (2) this device
must acceptany interference received, includinginterference that maycause undesired
operation.
Note:
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses,and canradiate radiofrequency energy and, if not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense.
The user is cautioned that any changes or modifications not expressly approved
by GE could void the user’s authority to operate the equipment.
Safety of machinery. General principles for design. Risk
assessment and risk reduction.
Degrees of protection provided by enclosures.EN60529, IEC60529
CLASS 1
LASER PRODUCT
EN60825-1 : 2014
CLASS 1
LASER PRODUCT
IEC60825-1:2014
1 Introduction
1.3 Regulatory information
1.3.5 Other regulations and standards
Regulations and standards,
products containing lasers
Subchapter J, Part
1040.10 Laser Products
This instrument is a class 1 Laser Product (IEC60825-1/EN60825-1). This instrument
meets the laser radiation safety requirements specified in the Code of the Federal Regulations (21 CFR, Chapter 1, Subchapter J).
DescriptionRegulation / Standard
Safety of laser productsEN/IEC 60825-1
Safety of laser productsUSA 21 CFR, Chapter I,
The following lasers can be installed in the Amersham Typhoon scanner:
Maximum powerOperating powerWavelengthLaser and class
70 mW25 mW (CW)488 nmLD laser, class 3B
80 mW10 mW (CW)532 nmSHG laser,class 3B
220 mW84 mW (CW)635 nmLD laser, class 3B
290 mW50 mW (CW)685 nmLD laser, class 3B
230 mW100 mW (CW)785 nmLD laser, class 3B
Note:
The beam divergence of all laser modules is collimated.
Different lasers are installed depending on the configuration, see Main components in
Amersham Typhoon scanner, on page 132.
14Amersham Typhoon Operating Instructions 29193226 AC
1.4Abbreviations
The abbreviations used in this Operating Instructions are defined as follows:
1 Introduction
1.4 Abbreviations
DescriptionAbbreviation
Two-dimensional Difference Gel Electrophoresis2D DIGE
Optical densityOD
Photo-multiplier tubePMT
Red Green BlueRGB
Relative humidityRH
RadioisotopeRI
Second harmonic generation nonlinear optical processSHG
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition,the chapterdescribes emergency and recovery procedures, and
provides recycling information.
WARNING
Before installing, operating or maintaining the product, all users
must read and understand the entire contents of this chapter
to become aware of the hazards involved.
In this chapter
See pageSection
172.1 Radiation
192.2 Safety precautions
272.3 Labels
332.4 Emergency procedures
362.5 Recycling information
372.6 Declaration of Hazardous Substances (DoHS)
16Amersham Typhoon Operating Instructions 29193226 AC
2.1Radiation
Radiation hazard prevention
This instrument is not equipped with any radioisotope or radiation generating unit, and
is therefore not regulated by radiationhazard prevention laws. However, the instrument
is capableof scanningstorage phosphorscreens which may be polluted by radioisotopes.
CAUTION
If radioisotope (RI) pollution occurs, stop use of the instrument immediately andfollow theinstructions of your radiation administrator.
Controlled area
Paragraph 1of Article1 ofthe LawEnforcement Rulesfor Prevention ofRadiation Hazards
due to Radioisotope and so forth (Prime Minister's Office ordinance No. 56) defines the
controlled area as "a place where the dose equivalent related to external radiation exceeds the dose equivalent determined by the Director General of the Science and
Technology Agency (hereinafter referred to as the Director General), the concentration
of radioisotopein theair exceedsthe concentrationdetermined by the Director General,
or the radioisotope density on the surface polluted by radioisotope exceeds the density
determined by the Director General."
2 Safety instructions
2.1 Radiation
Limit of superficial pollution
Paragraph 3 of Article 4 of Notice No. 15 of the Science and Technology Agency that
determines thequantity, etc.of radiatingisotope specifiesthat thedensity of radioisotope
on thesurface polluted by radioisotope must be one tenth of the density defined in Article
8.
Article 8 and Table 3 of this Notice define the limits as shown below:
1
Superficial density of radioisotope that radiates alpha rays: 4 Bq/cm
2
Superficial density of radioisotope that does not radiate alpha rays: 40 Bq/cm
This instrument is capable of scanning not only storage phosphor screens but also fluorescent pigment label samples (non-RI method). Therefore, it is recommended that the
user should install it outside the controlled area and use RI-indicated samples without
contacting them with storage phosphor screens directly.
However, as described above, the storage phosphor screen surface may be polluted by
radioisotope (RI), depending on the sample condition, since the instrument sticks the
sample to the3H-compatible storage phosphor screen surface and exposes it in an
auto-radiography experiment of the3H label sample.
The degree of superficial pollution of the storage phosphor screen is greatly influenced
by thedryness of thesample and doseof radioisotopein an experimentand may exceed
the limits mentioned in the section above.
When the instrument reads a storage phosphor screen with a polluted non-exposure
area, it may be polluted. The degree of such superficial pollution greatly differs with
users' operationconditions. Superficialpollution mayexceed thelimit mentionedabove.
Note:
As mentioned above, install this instrument in the RI controlled area if the user
uses RI-indicated samples that will be in direct contact with storage phosphor
screens.
Removal from the controlled
area
If it is necessary to move the instrument and its laboratory, which were installed and
have been used in the controlled area, from the controlled area, make sure that the degree ofthe superficial pollution is below the limits mentioned in Limit of superficial pollu-
tion, on page 17 above.
18Amersham Typhoon Operating Instructions 29193226 AC
2.2Safety precautions
Introduction
Amersham Typhoon scanner is powered by mains voltage and handles materials that
may be hazardous. Before installing, operating or maintaining the system, you must be
aware of the hazards described in this manual.
Follow the instructions provided to avoid personal injuries, damage to the product ,
or to other personnel and equipment in the area.
The safety precautions in this section are grouped into the following categories:
•
General precautions
•
Personal protection
•
Power supply
•
Installing and moving the system
•
System operation
•
Maintenance
2 Safety instructions
2.2 Safety precautions
General precautions
WARNING
Do notuse theequipment ifsmoke, strangenoises orstrange odors
can be perceived, or if the equipment becomes unusually hot. This
may result in fire or electric shock.
Stop using the equipment immediately, turn off the power switch
and unplug the equipment from the power outlet. Contact your
local GE representative to request repair.
WARNING
Do notdamage thepower supplycord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local GE
representative for replacements.
Do notallow liquids,flammable materialsor metallicobjects to get
into theAmersham Typhoon.This mayresult infire or electric shock.
In case of malfunction, turn off the power switch, unplug the
equipment from thepower outlet, thencontact you localGE representative.
WARNING
Never detach the inner cover screwed to this instrument. If it is
detached, laser beam may leak with a risk of loss of vision.
WARNING
Never modify the interlocks in this instrument, laser beam may leak
with a risk of loss of vision.
CAUTION
If radioisotope (RI) pollution occurs, stop use of the instrument immediately andfollow theinstructions of your radiation administrator.
CAUTION
Use of controls or adjustments or performance of procedures
other thanthose specifiedherein mayresult in hazardous radiation
exposure.
NOTICE
Avoid anyshock orvibration tothe equipment, asthis maydamage
the equipment.
20Amersham Typhoon Operating Instructions 29193226 AC
Personal protection
Power supply
2 Safety instructions
2.2 Safety precautions
CAUTION
Always wear gloves, protective glasses and a lab coat or similar
when handling samples.
CAUTION
Always wear cotton gloves when handling storage phosphor
screens.
WARNING
Do not use the equipment with a power supply other than that
recommended. Fire and electric shock could result.
WARNING
The Amersham Typhoon scanner must always be connected to a
grounded power outlet.
WARNING
Do not block access to the power switch and power cord. The
power switch must always be easy to access. The power cord with
plug must always be easy to disconnect.
WARNING
Connect thepower supplydirectly to a grounded wallpower outlet.
The useof extension cords ormultiple loads on one electrical outlet
could result in fire and electric shock.
Do notuse the samepower supplyas that of large equipmentsuch
as an air conditioner or centrifuge. Malfunction could result.
WARNING
Do not block the ventilation inlets or outlets on the system.
WARNING
Do not place the equipment on unstable tables or on inclined surfaces, asthe equipment couldbe droppedor fall, resultingin injury.
CAUTION
Only authorized service personnel are allowed to install or move
Amersham Typhoon scanner.Contact your localGE representative
for help and advice.
CAUTION
Install the Amersham Typhoon scanner in a location where it will
not come into contact with water or chemicals.
22Amersham Typhoon Operating Instructions 29193226 AC
2 Safety instructions
2.2 Safety precautions
CAUTION
•
Do not connect any USB devices other than the
Amersham Typhoon scanner to the computer in which the
Amersham Typhoon Control Software is installed. Malfunction
could result.
•
Use only the Amersham Typhoon Control Software during
scanning.
CAUTION
Connect the computer hardware on the same power circuit as the
scanner, otherwise the equipment may be influenced by electrical
noise.
CAUTION
Do not block the cooling fans. If they are blocked, the instrument
may malfunction.
NOTICE
Any computer used with the equipment must comply with EN/IEC
60950-1, andbe installedand usedaccording to themanufacturer's
instructions.
Operation
WARNING
Do not use the instrumentwithin or near a sink, or in humid(above
70% RH)or dustyenvironments. Fire andelectric shockcould result.
When opening or closing the main scanner door, make sure that
no objects or body parts are caught in the main scanner door.
CAUTION
Do not place heavy objects on the instrument, these may fall off
and cause injury.
CAUTION
Do not open the main scanner door or filter door while the device
is in operation. Injury could result.
CAUTION
Do notscratch or dropparts containingglass suchas lenses, filters
or lights.
CAUTION
Handle the sample stages and the digitization plate with care to
avoid dropping them.
CAUTION
Be careful not to break the glass surface of the fluor stage.
CAUTION
Reagents usedto prepare thesample should be used in accordance
with the manufacturer instructions.
24Amersham Typhoon Operating Instructions 29193226 AC
2 Safety instructions
2.2 Safety precautions
CAUTION
Do notleave samples inthe instrumentafter scanning. Ifleft, these
may degrade and cause damage to the instrument.
CAUTION
Do not insert a storage phosphor screen in Amersham Typhoon
before turning on the machine. If a storage phosphor screen is
detected during the self-diagnosis of the Amersham Typhoon, the
sensitivity of the storage phosphor screen may deteriorate and
reduce the quality of the scanned data.
CAUTION
Do not turn off power during operation as this can cause loss of
data in the internal memory. Only turn off power in an emergency
situation.
CAUTION
Do not use the instrumentwithin or near a sink, or in humid(above
70% RH) or dusty environments. This can result in data failure.
NOTICE
The automatic sleep mode of the computer should be turned off
to prevent the computer from entering sleep mode during a scan.
Otherwise loss of data might occur.
Maintenance
WARNING
Do not attempt to modify the instrument, or fire and electric shock
could result.
Do not use excessive amounts of liquids for cleaning the
Amersham Typhoon, this may result in product malfunction or
electric shock.
CAUTION
Wear gloves to prevent direct contact with chemical substances.
CAUTION
Take care when connectingthe power supplycable. Do not tug on
the cable,and do not handle the connection plugs with wethands.
CAUTION
Turn off the power switch and remove connecting cables before
moving the equipment.
CAUTION
Turn the power switch off before cleaning the inside of the equipment.
CAUTION
Unplug the equipment if it will not be used for an extended period.
26Amersham Typhoon Operating Instructions 29193226 AC
2.3Labels
About this section
This section describes the system label and other safety or regulatory labels that are
attached to Amersham Typhoon scanner.
System label
The illustration below shows an example of a system label that is attached to
Amersham Typhoonscanner. The system label identifies the productand showselectrical
data and regulatory compliance.
2 Safety instructions
2.3 Labels
DescriptionLabel text
Instrument modelModel
Instrument serial numberSerial number
Manufacturing year and monthManufacturing
Year and Month
Supply voltage frequencyFrequency
Supply voltageSupply Voltage
Max. current consumptionMax Current
Protection class. Ingress protection according to IEC 60529.Protection class
Warning! Read the user documentation before using the system. Donot openany covers or replaceparts unless specifically
stated in the user documentation.
The system complies with applicable European directives.
This symbol indicates that waste electrical and electronic
equipment must not be disposed as unsorted municipal waste
and mustbe collectedseparately. Pleasecontact anauthorized
representative of the manufacturer forinformation concerning
the decommissioning of equipment.
The systemcomplies withthe requirements for electromagnetic
compliance (EMC) in Australia and New Zealand.
This symbolindicates RRA registration ofcompatibility inKorea.
The registration number of the product will appear beside the
symbol. This equipment has been tested and found to comply
with the limits for a Class A digital device.
Eurasian Conformitymark: thesingle conformitymark indicates
that the product is approved for circulation on the markets of
the member states of the Eurasian Customs Union.
This symbol indicates that the product contains hazardous
materials in excess of the limits established by the Chinese
standard SJ/T11364-2014 Requirements for ConcentrationLimits for Certain Hazardous Substances in Electronics.
This symbol indicates that Amersham Typhoon scanner has
been certified by a Nationally Recognized Testing Laboratory
(NRTL).
28Amersham Typhoon Operating Instructions 29193226 AC
Labels concerning lasers
This device complies with part 15 of the FCC Rules.
Operation is subject to the following two conditions:
(1) This device may not cause harmful interference, and
(2) this device must accept any interference received,
including interference that may cause undersired operation.
CAN ICES-3 (A)/NMB-3(A)
2 Safety instructions
2.3 Labels
MeaningLabel
Warning! The instrument containslasers
and service is only allowed by trained
service engineers. Do not modify the instrument in any way.
This product complies with FDA performance standards for laser products except for deviations pursuant to Laser
Notice No. 50, dated (June 24, 2007).