GE Amersham Typhoon Operating Instructions Manual

Amersham

™

Typhoon

Operating Instructions

Original instructions

Table of Contents

Table of Contents

41 Introduction ..........................................................................................................

51.1 About this manual ................................................................................................................................

61.2 Important user information .............................................................................................................

81.3 Regulatory information ......................................................................................................................

91.3.1 EU Directives ....................................................................................................................................

101.3.2 Eurasian Customs Union ............................................................................................................

111.3.3 Regulations for USA and Canada ...........................................................................................

121.3.4 Korean regulatory information ................................................................................................

131.3.5 Other regulations and standards ............................................................................................

151.4 Abbreviations ..........................................................................................................................................

162 Safety instructions ...............................................................................................

172.1 Radiation ...................................................................................................................................................

192.2 Safety precautions ...............................................................................................................................

272.3 Labels .........................................................................................................................................................

332.4 Emergency procedures ......................................................................................................................

362.5 Recycling information .........................................................................................................................

372.6 Declaration of Hazardous Substances (DoHS) ........................................................................

393 System description ..............................................................................................

403.1 System overview ...................................................................................................................................

433.2 Illustrations ..............................................................................................................................................

473.3 Stages ........................................................................................................................................................

483.3.1 Overview of stages ........................................................................................................................

493.3.2 Fluor stage with accessories .....................................................................................................

533.3.3 Phosphor stage with accessories ...........................................................................................

553.3.4 Multi stage with accessories .....................................................................................................

573.4 Filters ..........................................................................................................................................................

584 Amersham Typhoon Control Software .............................................................

594.1 Main window ...........................................................................................................................................

664.2 Scanning modes ...................................................................................................................................

694.3 General settings ....................................................................................................................................

724.4 Scan area functions .............................................................................................................................

764.5 Image file settings ................................................................................................................................

784.6 View Image mode .................................................................................................................................

815 Installation ............................................................................................................

825.1 Site requirements ..................................................................................................................................

855.2 Delivery and transport .......................................................................................................................

865.3 Setup ...........................................................................................................................................................

885.4 Connections ............................................................................................................................................

2 Amersham Typhoon Operating Instructions 29193226 AC

Table of Contents

916 Operation ..............................................................................................................

936.1 Preparing the Amersham Typhoon scanner ............................................................................

946.1.1 Starting the system .......................................................................................................................

976.1.2 Changing the filters .......................................................................................................................

1016.1.3 Custom filters ...................................................................................................................................

1036.2 Preparing the sample .........................................................................................................................

1046.2.1 Preparing a sample for fluorescence imaging ..................................................................

1096.2.2 Preparing a sample for phosphor imaging ........................................................................

1166.2.3 Preparing a sample for densitometry ...................................................................................

1176.3 Performing a scan ................................................................................................................................

1226.4 Procedures after a scan ....................................................................................................................

1247 Maintenance .........................................................................................................

1278 Troubleshooting ...................................................................................................

1288.1 General errors and warnings ..........................................................................................................

1298.2 Error messages ......................................................................................................................................

1319 Reference information ........................................................................................

1329.1 Specifications .........................................................................................................................................

1359.2 Software ....................................................................................................................................................

1369.3 Ordering information ..........................................................................................................................

1389.4 Health and Safety Declaration Forms .........................................................................................

140Index .......................................................................................................................

Amersham Typhoon Operating Instructions 29193226 AC 3

1 Introduction

1 Introduction

About this chapter

This chaptercontains importantuser information,descriptions ofsafety notices,regula­tory information, and intended use of the Amersham Typhoon system.

In this chapter

This chapter contains the following sections:

See pageSection

51.1 About this manual

61.2 Important user information

81.3 Regulatory information

151.4 Abbreviations

4 Amersham Typhoon Operating Instructions 29193226 AC

1.1 About this manual

Purpose of this manual

The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.

Scope of this manual

The Operating Instructions cover all configurations of Amersham Typhoon scanner and
the Amersham Typhoon Control Software. The illustration below shows the
Amersham Typhoon scanner.

1 Introduction

1.1 About this manual

Descriptions of Amersham Eraser and Amersham Cabinet are not covered in this docu­ment.

Typographical conventions

Software items are identified in the text by bold italic text.

Hardware items are identified in the text by bold text (for example, Power switch).

Amersham Typhoon Operating Instructions 29193226 AC 5

1 Introduction

1.2 Important user information

1.2 Important user information

Read this before operating the
product

All users must read the entire Operating Instructions before installing, operating or
maintaining the product.

Always keep the Operating Instructions at hand when operating the product.

Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.

Intended use of the
Amersham Typhoon scanner

The Amersham Typhoon scanner is a versatile laser scanner that can be used for many
different imaging applications, including:

•

imaging of storage phosphor screens

•

fluorescence imaging with multiple lasers, for example for muliplex Western blotting
and two-dimensional difference gel electrophoresis (2D DIGE)

•

imaging of gels with colorimetric stains

•

densitometric imaging, for example of Coomassie™ blue stained gels

Amersham Typhoon scanner is intended for research use only, and shall not be used in
any clinical procedures or for diagnostic purposes.

Prerequisites

In order to operate Amersham Typhoon scanner in the way it is intended, the following
prerequisites must be fulfilled:

•

You must have read and understood the safety instructions in the user documenta­tion.

•

All operations should be performed by qualified personnel who are adequately
trained.

6 Amersham Typhoon Operating Instructions 29193226 AC

Safety notices

1 Introduction

1.2 Important user information

•

Amersham Typhoon scanner must be installed in accordance with the site require­ments and instructions in Chapter 5 Installation, on page 81.

This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.

WARNING

WARNING indicates a hazardous situation which, if not avoided,

could resultin deathor serious injury. It is important notto proceed
until all stated conditions are met and clearly understood.

CAUTION

CAUTION indicates a hazardous situation which, if not avoided,

could result in minor or moderate injury. It is important not to pro­ceed until all stated conditions are met and clearly understood.

NOTICE

NOTICE indicates instructions that must be followed to avoid

damage to the product or other equipment.

Notes and tips

Note:

Tip:

Amersham Typhoon Operating Instructions 29193226 AC 7

A note is used to indicate information that is important for trouble-free and
optimal use of the product .

A tip contains useful information that can improve or optimize your procedures.

1 Introduction

1.3 Regulatory information

1.3 Regulatory information

Introduction

This sectionlists theregulations and standards that applyto Amersham Typhoon scanner.

Manufacturing information

The table below summarizes the required manufacturing information.

In this section

InformationRequirement

GE Healthcare Bio-Sciences AB,Name and address of manufacturer

Björkgatan 30, SE 751 84 Uppsala, Sweden

See pageSection

91.3.1 EU Directives

101.3.2 Eurasian Customs Union

111.3.3 Regulations for USA and Canada

121.3.4 Korean regulatory information

131.3.5 Other regulations and standards

8 Amersham Typhoon Operating Instructions 29193226 AC

1.3.1 EU Directives

Conformity with EU Directives

This product fulfills the European Directives listed below. See the EU Declaration of
Conformity for the directives and regulations that apply for the CE marking.

If not included with the product, a copy of the EU Declaration of Conformity is available
on request.

CE marking

1 Introduction

1.3 Regulatory information

1.3.1 EU Directives

TitleDirective

Machinery Directive (MD)2006/42/EC

Electromagnetic Compatibility (EMC) Directive2014/30/EU

Low Voltage Directive (LVD)2014/35/EU

Restriction of Hazardous Substances (RoHS) Directive2011/65/EU

The CE marking and the corresponding EU Declaration of Conformity is valid for the in­strument when it is:

•

used according to the Operating Instructions or user manuals, and

•

used in the same state as it was delivered from GE, except for alterations described
in the Operating Instructions or user manuals.

Amersham Typhoon Operating Instructions 29193226 AC 9

1 Introduction

1.3 Regulatory information

1.3.2 Eurasian Customs Union

1.3.2 Eurasian Customs Union

Introduction

This section contains additional regulatory information to comply with the Eurasian
Customs Union technical regulations.

Manufacturer and importer
information

The table below summarizes the manufacturer and importer information required by
the Eurasian Customs Union.

ing information about importer

InformationRequirement

See Manufacturing informationName and address of manufacturer

Telephone: + 46 771 400 600Telephone number of manufacturer

GE Healthcare LLCImporter and/orcompany forobtain-

GE Healthcare Life Sciences

Presnenskaya nab., 10C, 12th floor

RU-123 317 Moscow, Russian Federation

Telephone 1: + 7 495 411 9714

Fax nr: + 7 495 739 6932

Email: [e-mail]

10 Amersham Typhoon Operating Instructions 29193226 AC

1.3.3 Regulations for USA and Canada

NRTL certification

This symbol indicates that Amersham Typhoon has been certified by a Nationally Rec­ognized Testing Laboratory (NRTL).

NRTL means an organization, which is recognized by the US Occupational Safety and
Health Administration (OSHA) as meeting the legal requirements of Title 29 of the Code
of Federal Regulations (29 CFR), Part 1910.7.

FCC compliance

This device complies with part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) This device may not cause harmful interference, and (2) this device
must acceptany interference received, includinginterference that maycause undesired
operation.

Note:

This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea­sonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses,and canradiate radiofrequen­cy energy and, if not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user will be re­quired to correct the interference at his own expense.

The user is cautioned that any changes or modifications not expressly approved
by GE could void the user’s authority to operate the equipment.

1 Introduction

1.3 Regulatory information

1.3.3 Regulations for USA and Canada

Amersham Typhoon Operating Instructions 29193226 AC 11

1 Introduction

1.3 Regulatory information

1.3.4 Korean regulatory information

1.3.4 Korean regulatory information

NOTICE

Class A equipment (equipment for business use).

This equipment has been evaluated for its suitability for use in a
business environment.

When usedin a residential environment, there is a concern of radio
interference.

주의사항

A급 기기 (업무용 방송통신 기자재)
이 기기는 업무용환경에서 사용할 목적으로 적합성평가를 받은

기기
로서 가정용 환경에서 사용하는 경우 전파간섭의 우려가 있습니

다.

12 Amersham Typhoon Operating Instructions 29193226 AC

1.3.5 Other regulations and standards

Environmental conformity

This product conforms to the following environmental requirements.

TitleRequirement

Waste Electrical and Electronic Equipment (WEEE) Directive2012/19/EU

1 Introduction

1.3 Regulatory information

1.3.5 Other regulations and standards

China RoHS

Standards, machinery and
electrical equipment

Harmonized standard requirements fulfilledby this product aresummarized in the table
below.

EN 61010-1, IEC 61010-1,
UL 61010-1, CAN/CSA­C22.2 No. 61010-1

IEC/EN 61010-2-081,
UL61010-2-081, CAN/CSA­C22.2 No. 61010-2-081

EN 61326-1, IEC 61326-1,
FCC Part 15 B Class A,
ICES-003 Class A

Management Methods for the Restriction of the Use of Haz­ardous Substances in Electrical and Electronic Products.

DescriptionStandard

Safety requirements for electrical equipment for mea­surement, control, and laboratory use - Part 1: General
requirements.

Particular requirements for automatic and semi-auto­matic laboratory equipment for analysis and other
purposes.

Electrical equipment for measurement, control and
laboratory use - EMC requirements.

NOTICE

This equipmentis notintended for
use in residential environments
and may not provide adequate
protection to radio reception in
such environments.

EN ISO 12100

Amersham Typhoon Operating Instructions 29193226 AC 13

Safety of machinery. General principles for design. Risk
assessment and risk reduction.

Degrees of protection provided by enclosures.EN60529, IEC60529

CLASS 1
LASER PRODUCT

EN60825-1 : 2014

CLASS 1
LASER PRODUCT

IEC60825-1:2014

1 Introduction

1.3 Regulatory information

1.3.5 Other regulations and standards

Regulations and standards,
products containing lasers

Subchapter J, Part

1040.10 Laser Products

This instrument is a class 1 Laser Product (IEC60825-1/EN60825-1). This instrument
meets the laser radiation safety requirements specified in the Code of the Federal Reg­ulations (21 CFR, Chapter 1, Subchapter J).

DescriptionRegulation / Standard

Safety of laser productsEN/IEC 60825-1

Safety of laser productsUSA 21 CFR, Chapter I,

The following lasers can be installed in the Amersham Typhoon scanner:

Maximum powerOperating powerWavelengthLaser and class

70 mW25 mW (CW)488 nmLD laser, class 3B

80 mW10 mW (CW)532 nmSHG laser,class 3B

220 mW84 mW (CW)635 nmLD laser, class 3B

290 mW50 mW (CW)685 nmLD laser, class 3B

230 mW100 mW (CW)785 nmLD laser, class 3B

Note:

The beam divergence of all laser modules is collimated.

Different lasers are installed depending on the configuration, see Main components in

Amersham Typhoon scanner, on page 132.

14 Amersham Typhoon Operating Instructions 29193226 AC

1.4 Abbreviations

The abbreviations used in this Operating Instructions are defined as follows:

1 Introduction

1.4 Abbreviations

DescriptionAbbreviation

Two-dimensional Difference Gel Electrophoresis2D DIGE

Optical densityOD

Photo-multiplier tubePMT

Red Green BlueRGB

Relative humidityRH

RadioisotopeRI

Second harmonic generation nonlinear optical processSHG

Titer plateTP

Ultraviolet radiationUV

Near-infrared radiationNIR

Amersham Typhoon Operating Instructions 29193226 AC 15

2 Safety instructions

2 Safety instructions

About this chapter

This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition,the chapterdescribes emergency and recovery procedures, and
provides recycling information.

WARNING

Before installing, operating or maintaining the product, all users
must read and understand the entire contents of this chapter
to become aware of the hazards involved.

In this chapter

See pageSection

172.1 Radiation

192.2 Safety precautions

272.3 Labels

332.4 Emergency procedures

362.5 Recycling information

372.6 Declaration of Hazardous Substances (DoHS)

16 Amersham Typhoon Operating Instructions 29193226 AC

2.1 Radiation

Radiation hazard prevention

This instrument is not equipped with any radioisotope or radiation generating unit, and
is therefore not regulated by radiationhazard prevention laws. However, the instrument
is capableof scanningstorage phosphorscreens which may be polluted by radioisotopes.

CAUTION

If radioisotope (RI) pollution occurs, stop use of the instrument im­mediately andfollow theinstructions of your radiation administra­tor.

Controlled area

Paragraph 1of Article1 ofthe LawEnforcement Rulesfor Prevention ofRadiation Hazards
due to Radioisotope and so forth (Prime Minister's Office ordinance No. 56) defines the
controlled area as "a place where the dose equivalent related to external radiation ex­ceeds the dose equivalent determined by the Director General of the Science and
Technology Agency (hereinafter referred to as the Director General), the concentration
of radioisotopein theair exceedsthe concentrationdetermined by the Director General,
or the radioisotope density on the surface polluted by radioisotope exceeds the density
determined by the Director General."

2 Safety instructions

2.1 Radiation

Limit of superficial pollution

Paragraph 3 of Article 4 of Notice No. 15 of the Science and Technology Agency that
determines thequantity, etc.of radiatingisotope specifiesthat thedensity of radioisotope
on thesurface polluted by radioisotope must be one tenth of the density defined in Article

8.

Article 8 and Table 3 of this Notice define the limits as shown below:

1

Superficial density of radioisotope that radiates alpha rays: 4 Bq/cm

2

Superficial density of radioisotope that does not radiate alpha rays: 40 Bq/cm

Amersham Typhoon Operating Instructions 29193226 AC 17

2

2

2 Safety instructions

2.1 Radiation

Installation site of instrument

This instrument is capable of scanning not only storage phosphor screens but also fluo­rescent pigment label samples (non-RI method). Therefore, it is recommended that the
user should install it outside the controlled area and use RI-indicated samples without
contacting them with storage phosphor screens directly.

However, as described above, the storage phosphor screen surface may be polluted by
radioisotope (RI), depending on the sample condition, since the instrument sticks the
sample to the3H-compatible storage phosphor screen surface and exposes it in an
auto-radiography experiment of the3H label sample.

The degree of superficial pollution of the storage phosphor screen is greatly influenced
by thedryness of thesample and doseof radioisotopein an experimentand may exceed
the limits mentioned in the section above.

When the instrument reads a storage phosphor screen with a polluted non-exposure
area, it may be polluted. The degree of such superficial pollution greatly differs with
users' operationconditions. Superficialpollution mayexceed thelimit mentionedabove.

Note:

As mentioned above, install this instrument in the RI controlled area if the user
uses RI-indicated samples that will be in direct contact with storage phosphor
screens.

Removal from the controlled
area

If it is necessary to move the instrument and its laboratory, which were installed and
have been used in the controlled area, from the controlled area, make sure that the de­gree ofthe superficial pollution is below the limits mentioned in Limit of superficial pollu-

tion, on page 17 above.

18 Amersham Typhoon Operating Instructions 29193226 AC

2.2 Safety precautions

Introduction

Amersham Typhoon scanner is powered by mains voltage and handles materials that
may be hazardous. Before installing, operating or maintaining the system, you must be
aware of the hazards described in this manual.

Follow the instructions provided to avoid personal injuries, damage to the product ,
or to other personnel and equipment in the area.

The safety precautions in this section are grouped into the following categories:

•

General precautions

•

Personal protection

•

Power supply

•

Installing and moving the system

•

System operation

•

Maintenance

2 Safety instructions

2.2 Safety precautions

General precautions

WARNING

Do notuse theequipment ifsmoke, strangenoises orstrange odors
can be perceived, or if the equipment becomes unusually hot. This
may result in fire or electric shock.

Stop using the equipment immediately, turn off the power switch
and unplug the equipment from the power outlet. Contact your
local GE representative to request repair.

WARNING

Do notdamage thepower supplycord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.

If the power supply cords are damaged, contact your local GE
representative for replacements.

Amersham Typhoon Operating Instructions 29193226 AC 19

2 Safety instructions

2.2 Safety precautions

WARNING

Do notallow liquids,flammable materialsor metallicobjects to get
into theAmersham Typhoon.This mayresult infire or electric shock.

In case of malfunction, turn off the power switch, unplug the
equipment from thepower outlet, thencontact you localGE repre­sentative.

WARNING

Never detach the inner cover screwed to this instrument. If it is
detached, laser beam may leak with a risk of loss of vision.

WARNING

Never modify the interlocks in this instrument, laser beam may leak
with a risk of loss of vision.

CAUTION

If radioisotope (RI) pollution occurs, stop use of the instrument im­mediately andfollow theinstructions of your radiation administra­tor.

CAUTION

Use of controls or adjustments or performance of procedures
other thanthose specifiedherein mayresult in hazardous radiation
exposure.

NOTICE

Avoid anyshock orvibration tothe equipment, asthis maydamage
the equipment.

20 Amersham Typhoon Operating Instructions 29193226 AC

Personal protection

Power supply

2 Safety instructions

2.2 Safety precautions

CAUTION

Always wear gloves, protective glasses and a lab coat or similar
when handling samples.

CAUTION

Always wear cotton gloves when handling storage phosphor
screens.

WARNING

Do not use the equipment with a power supply other than that
recommended. Fire and electric shock could result.

WARNING

The Amersham Typhoon scanner must always be connected to a
grounded power outlet.

WARNING

Do not block access to the power switch and power cord. The
power switch must always be easy to access. The power cord with
plug must always be easy to disconnect.

WARNING

Connect thepower supplydirectly to a grounded wallpower outlet.
The useof extension cords ormultiple loads on one electrical outlet
could result in fire and electric shock.

Amersham Typhoon Operating Instructions 29193226 AC 21

2 Safety instructions

2.2 Safety precautions

Installing and moving

WARNING

Only use power cords delivered or approved by GE.

CAUTION

Do notuse the samepower supplyas that of large equipmentsuch
as an air conditioner or centrifuge. Malfunction could result.

WARNING

Do not block the ventilation inlets or outlets on the system.

WARNING

Do not place the equipment on unstable tables or on inclined sur­faces, asthe equipment couldbe droppedor fall, resultingin injury.

CAUTION

Only authorized service personnel are allowed to install or move
Amersham Typhoon scanner.Contact your localGE representative
for help and advice.

CAUTION

Install the Amersham Typhoon scanner in a location where it will
not come into contact with water or chemicals.

22 Amersham Typhoon Operating Instructions 29193226 AC

2 Safety instructions

2.2 Safety precautions

CAUTION

•

Do not connect any USB devices other than the
Amersham Typhoon scanner to the computer in which the
Amersham Typhoon Control Software is installed. Malfunction
could result.

•

Use only the Amersham Typhoon Control Software during
scanning.

CAUTION

Connect the computer hardware on the same power circuit as the
scanner, otherwise the equipment may be influenced by electrical
noise.

CAUTION

Do not block the cooling fans. If they are blocked, the instrument
may malfunction.

NOTICE

Any computer used with the equipment must comply with EN/IEC
60950-1, andbe installedand usedaccording to themanufacturer's
instructions.

Operation

WARNING

Do not use the instrumentwithin or near a sink, or in humid(above
70% RH)or dustyenvironments. Fire andelectric shockcould result.

Amersham Typhoon Operating Instructions 29193226 AC 23

2 Safety instructions

2.2 Safety precautions

WARNING

When opening or closing the main scanner door, make sure that
no objects or body parts are caught in the main scanner door.

CAUTION

Do not place heavy objects on the instrument, these may fall off
and cause injury.

CAUTION

Do not open the main scanner door or filter door while the device
is in operation. Injury could result.

CAUTION

Do notscratch or dropparts containingglass suchas lenses, filters
or lights.

CAUTION

Handle the sample stages and the digitization plate with care to
avoid dropping them.

CAUTION

Be careful not to break the glass surface of the fluor stage.

CAUTION

Reagents usedto prepare thesample should be used in accordance
with the manufacturer instructions.

24 Amersham Typhoon Operating Instructions 29193226 AC

2 Safety instructions

2.2 Safety precautions

CAUTION

Do notleave samples inthe instrumentafter scanning. Ifleft, these
may degrade and cause damage to the instrument.

CAUTION

Do not insert a storage phosphor screen in Amersham Typhoon
before turning on the machine. If a storage phosphor screen is
detected during the self-diagnosis of the Amersham Typhoon, the
sensitivity of the storage phosphor screen may deteriorate and
reduce the quality of the scanned data.

CAUTION

Do not turn off power during operation as this can cause loss of
data in the internal memory. Only turn off power in an emergency
situation.

CAUTION

Do not use the instrumentwithin or near a sink, or in humid(above
70% RH) or dusty environments. This can result in data failure.

NOTICE

The automatic sleep mode of the computer should be turned off
to prevent the computer from entering sleep mode during a scan.
Otherwise loss of data might occur.

Maintenance

WARNING

Do not attempt to modify the instrument, or fire and electric shock
could result.

Amersham Typhoon Operating Instructions 29193226 AC 25

2 Safety instructions

2.2 Safety precautions

WARNING

Do not use excessive amounts of liquids for cleaning the
Amersham Typhoon, this may result in product malfunction or
electric shock.

CAUTION

Wear gloves to prevent direct contact with chemical substances.

CAUTION

Take care when connectingthe power supplycable. Do not tug on
the cable,and do not handle the connection plugs with wethands.

CAUTION

Turn off the power switch and remove connecting cables before
moving the equipment.

CAUTION

Turn the power switch off before cleaning the inside of the equip­ment.

CAUTION

Unplug the equipment if it will not be used for an extended period.

26 Amersham Typhoon Operating Instructions 29193226 AC

2.3 Labels

About this section

This section describes the system label and other safety or regulatory labels that are
attached to Amersham Typhoon scanner.

System label

The illustration below shows an example of a system label that is attached to
Amersham Typhoonscanner. The system label identifies the productand showselectrical
data and regulatory compliance.

2 Safety instructions

2.3 Labels

DescriptionLabel text

Instrument modelModel

Instrument serial numberSerial number

Manufacturing year and monthManufacturing

Year and Month

Supply voltage frequencyFrequency

Supply voltageSupply Voltage

Max. current consumptionMax Current

Protection class. Ingress protection according to IEC 60529.Protection class

Warning! Read the user documentation before using the sys­tem. Donot openany covers or replaceparts unless specifically
stated in the user documentation.

Amersham Typhoon Operating Instructions 29193226 AC 27

2 Safety instructions

2.3 Labels

DescriptionLabel text

The system complies with applicable European directives.

This symbol indicates that waste electrical and electronic
equipment must not be disposed as unsorted municipal waste
and mustbe collectedseparately. Pleasecontact anauthorized
representative of the manufacturer forinformation concerning
the decommissioning of equipment.

The systemcomplies withthe requirements for electromagnetic
compliance (EMC) in Australia and New Zealand.

This symbolindicates RRA registration ofcompatibility inKorea.
The registration number of the product will appear beside the
symbol. This equipment has been tested and found to comply
with the limits for a Class A digital device.

Eurasian Conformitymark: thesingle conformitymark indicates
that the product is approved for circulation on the markets of
the member states of the Eurasian Customs Union.

This symbol indicates that the product contains hazardous
materials in excess of the limits established by the Chinese
standard SJ/T11364-2014 Requirements for Concentration
Limits for Certain Hazardous Substances in Electronics.

This symbol indicates that Amersham Typhoon scanner has
been certified by a Nationally Recognized Testing Laboratory
(NRTL).

28 Amersham Typhoon Operating Instructions 29193226 AC

Labels concerning lasers

This device complies with part 15 of the FCC Rules.
Operation is subject to the following two conditions:
(1) This device may not cause harmful interference, and
(2) this device must accept any interference received,
including interference that may cause undersired operation.

CAN ICES-3 (A)/NMB-3(A)

2 Safety instructions

2.3 Labels

MeaningLabel

Warning! The instrument containslasers

and service is only allowed by trained
service engineers. Do not modify the in­strument in any way.

This product complies with FDA perfor­mance standards for laser products ex­cept for deviations pursuant to Laser
Notice No. 50, dated (June 24, 2007).

Other safety labels

Amersham Typhoon Operating Instructions 29193226 AC 29

MeaningLabel

This device complies with part 15 of the
FCC rules.

CAN ICES-3 (A)/NMB-3 (A)

2 Safety instructions

2.3 Labels

Symbols on the
Amersham Typhoon scanner

MeaningLabel

On/Off switch

Power switch

l: Power ON

O: Power OFF

USB port

30 Amersham Typhoon Operating Instructions 29193226 AC

Location of labels and symbols
on the Amersham Typhoon
scanner

2 Safety instructions

2.3 Labels

Amersham Typhoon Operating Instructions 29193226 AC 31

2 Safety instructions

2.3 Labels

Interlock parts inside the
Amersham Typhoon scanner

Never insertany foreignobjects into the interlock parts to avoid potential laser exposure
and injury.

32 Amersham Typhoon Operating Instructions 29193226 AC

2.4 Emergency procedures

About this section

This sectiondescribes how to do an emergency shutdownof AmershamTyphoonscanner.
The section also describes the results of a power failure.

Precautions

WARNING

Access to power switch and power cord with plug. Do not block

access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.

2 Safety instructions

2.4 Emergency procedures

Amersham Typhoon Operating Instructions 29193226 AC 33

2 Safety instructions

2.4 Emergency procedures

Emergency shutdown

In an emergency situation, follow the steps below to stop the scan:

ActionStep

Click the Stop button in the Amersham Typhoon Control Software.1

2

Turn off the Amersham Typhoon scanner by switching the power switch on
the right side of the instrument to the O position.

Disconnect the power cord from the wall socket.3

34 Amersham Typhoon Operating Instructions 29193226 AC

Power failure

2 Safety instructions

2.4 Emergency procedures

The following table describes the consequences of a power failure.

will result in...Power failure to...

Amersham Typhoon
scanner

Computer running the
control software

Amersham Typhoon
scanner and computer
running the control soft­ware

Restart after emergency
shutdown or power failure

Follow theinstructions torestart the instrument after an emergency shutdownor power
failure.

ActionStep

1

Make sure that the condition that caused the emergency shutdown or
power failure is corrected.

The scanis interruptedimmediately. Theinstrument

•
is in an undefined state.

The data collected up to the time of the power fail-

•
ure is available in the file created when starting the
scan.

The computer shuts down immediately.

•

The run continues, but no data is saved.

•

The scanis interruptedimmediately. Theinstrument

•
is in an undefined state.

The computer shutsdown immediately andno data

•
is saved.

2

3

Amersham Typhoon Operating Instructions 29193226 AC 35

If powerto theinstrument hasbeen lost, re-start the instrument as described
in Start the Amersham Typhoon scanner, on page 94.

Re-start the computer and Amersham Typhoon Control Software as de­scribed in Start the Amersham Typhoon Control Software, on page 95.

2 Safety instructions

2.5 Recycling information

2.5 Recycling information

Introduction

This section contains information about the decommisioning of Amersham Typhoon
scanner.

Decontamination

The product must be decontaminated before decommissioning. All local regulations
must be followed with regard to scrapping of the equipment.

General instructions for disposal

When taking Amersham Typhoon scanner out of service, the different materials must
be separated and recycled according to national and local environmental regulations.

Specific instructions for disposal

Measure the superficial radio isotope pollution of the instrument and storage phosphor
screen as mentioned in the Radiation hazard prevention, on page 17.

If the pollution level exceeds the limit, dispose of the instrument as radioactive waste.
Otherwise, dispose of the materials according to applicable laws and regulations for
disposal of industrial waste.

Disposal of electrical
components

Waste ofelectrical andelectronic equipment must not be disposed as unsorted municipal
waste and must be collected separately. Please contact an authorized representative
of themanufacturerfor information concerningthe decommissioning ofthe equipment.

36 Amersham Typhoon Operating Instructions 29193226 AC

2 Safety instructions

2.6 Declaration of Hazardous Substances (DoHS)

2.6 Declaration of Hazardous Substances (DoHS)

根据SJ/T11364-2014《电子电气产品有害物质限制使用标识要求》特提供如下有关污染控制方面的
信息。

The following product pollution control information is provided according to SJ/T11364-2014 Marking
for Restriction of Hazardous Substances caused by electrical and electronic products.

电子信息产品污染控制标志说明

Explanation of Pollution Control Label

该标志表明本产品含有超过中国标准GB/T 26572 《电子电气产品中限用物质的限
量要求 》中限量的有害物质。标志中的数字为本产品的环保使用期，表明本产品
在正常使用的条件下，有毒有害物质不会发生外泄或突变，用户使用本产品不会
对环境造成严重污染或对其人身、财产造成严重损害的期限。单位为年。

为保证所申明的环保使用期限，应按产品手册中所规定的环境条件和方法进行正
常使用，并严格遵守产品维修手册中规定的定期维修和保养要求。

产品中的消耗件和某些零部件可能有其单独的环保使用期限标志，并且其环保使
用期限有可能比整个产品本身的环保使用期限短。应到期按产品维修程序更换那
些消耗件和零部件，以保证所申明的整个产品的环保使用期限。

本产品在使用寿命结束时不可作为普通生活垃圾处理，应被单独收集妥善处理。

This symbolindicates theproduct containshazardous materials in excessof thelimits
established by the Chinese standard GB/T 26572 Requirements of concentration
limits for certain restricted substances in electrical and electronic products. The
number in the symbol is the Environment-friendly Use Period (EFUP), which indicates
the period during which the hazardous substances contained in electrical and elec­tronic products will not leak or mutate under normal operating conditionsso that the
use ofsuch electricaland electronicproducts willnot result inany severeenvironmen­tal pollution,any bodily injury or damage to anyassets. Theunit of the period is “Year”.

In order to maintain the declared EFUP, the product shall be operated normally ac­cording to the instructions and environmental conditions as defined in the product
manual, and periodic maintenance schedules specified in Product Maintenance Pro­cedures shall be followed strictly.

Consumables or certain parts may have their own label with an EFUP value less than
the product. Periodic replacement of those consumables or parts to maintain the
declared EFUP shall be done in accordancewith theProduct MaintenanceProcedures.

This product must not be disposed of as unsorted municipal waste, and must be
collected separately and handled properly after decommissioning.

Amersham Typhoon Operating Instructions 29193226 AC 37

2 Safety instructions

2.6 Declaration of Hazardous Substances (DoHS)

有害物质的名称及含量

Name and Concentration of Hazardous Substances

产品中有害物质的名称及含量

Table of Hazardous Substances’ Name and Concentration

部件名称

Component name

本表格依据SJ/T 11364的规定编制。

This table is prepared according to SJ/T 11364.

0:

X:

•

0:

X:

表示该有害物质在该部件所有均质材料中的含量均在 GB/T 26572规定的限量要求以下。

表示该有害物质至少在该部件的某一均质材料中的含量超出 GB/T 26572规定的限量要
求。

此表所列数据为发布时所能获得的最佳信息。

Indicates that this hazardous substance contained in all of the homogeneous materials for
this part is below the limit requirement in GB/T 26572.

Indicates that this hazardous substance contained in at least one of the homogeneous
materials used for this part is above the limit requirement in GB/T 26572.

有害物质

Hazardous substance

铅

(Pb)

汞

(Hg)

镉

(Cd)

六价铬

(Cr(VI))

多溴联苯

(PBB)

多溴二苯醚

(PBDE)

000X0X29187191

000X0X29187193

00000X29187194

000X0X29238583

•

38 Amersham Typhoon Operating Instructions 29193226 AC

Data listed in the table represents best information available at the time of publication.

3 System description

About this chapter

This chapter gives an overview of Amersham Typhoon scanner and a brief description
of its function.

In this chapter

3 System description

See pageSection

403.1 System overview

433.2 Illustrations

473.3 Stages

573.4 Filters

Amersham Typhoon Operating Instructions 29193226 AC 39

1

2

3

3 System description

3.1 System overview

3.1 System overview

Introduction

The Amersham Typhoon scanner is a laser scanner that can be used for many different
imaging applications. The scanner is part of the complete Amersham Typhoon system,
that consists of the scanner, an eraser and a cabinet.

For moreinformation on how to safely operate the Amersham Eraser, read theAmersham
Eraser Operating Instructions, 29187307.

Scanner configurations

The Amersham Typhoon scanner is available in four different configurations:

FunctionPart

Amersham Typhoon scanner1

Amersham Eraser2

Amersham Cabinet3

Amersham Typhoon scanner RGB

Intended useConfiguration

Phosphor imagingAmersham Typhoon scanner IP

Fluorescence with three lasers, phosphor
imaging, and densitometry

40 Amersham Typhoon Operating Instructions 29193226 AC

3 System description

3.1 System overview

Intended useConfiguration

Amersham Typhoon scanner 5

Amersham Typhoon scanner NIR

For an overview of the types of lasers that are installed, refer to Main components in

Amersham Typhoon scanner, on page 132 and Laser specifications, on page 132.

Scanning modes

The AmershamTyphoon scanner canbe usedfor scanning withthree different scanning
modes.

Fluorescence

Phosphor imaging

Fluorescence with five lasers, phosphor
imaging, and densitometry

Fluorescence with two lasers, 685 nm
and 785 nm, for NIR fluorescence appli­cations.

Used forMode

Scanning fluorescent samplesusing different combina-

•
tions of lasers and filters.

Scanning luminescent samples, with all the lasers

•
turned off and no filters in use.

Scanning storage phosphor screens that have been ex­posed to radioactive samples, to image and quantify radi­oligands in the samples.

Densitometry

Scanning gels to obtain digital images of gels for doc-

•
umentation.

Densitometric imaging of stained gels (e.g., Coom-

•
massie-stained gels) for quantitation of samples.

Photomultiplier tubes

The photomultipliertube (PMT)detects lightand converts it to an electric signal. The PMT
potential (in volt) determines the amplitude of the signal.

Different types of photomultiplier tubesare installed in the AmershamTyphoon scanner:

•

Bi-alkali

•

Multi-alkali

Amersham Typhoon Operating Instructions 29193226 AC 41

3 System description

3.1 System overview

The bi-alkali PMT is very sensitive in the 400-500 nm wavelength region. It is ideally
suited for phosphor imaging.

The multi-alkaliPMT hasa wide spectral responsefrom theultraviolet tothe nearinfrared
region. It is the detector of choice for fluorescence applications.

Cooling fans

The AmershamTyphoon scanner hascooling fansthat prevent theinternal temperature
from rising.

Instrument control

The AmershamTyphoon scanner is operated usingAmersham TyphoonControl Software.
The scanner also has an instrument panel with indicator lamps that indicate the status
of the instrument. Refer to Instrument panel, on page 45 for more information about the
instrument panel.

42 Amersham Typhoon Operating Instructions 29193226 AC

3.2 Illustrations

1

2

3

4

5

6

7

8

Front and right view of the
Amersham Typhoon scanner

3 System description

3.2 Illustrations

FunctionPart

Instrument panel1

Main scanner door2

Scanner door handle3

Instrument cover4

Power cord connector5

Power switch6

Filter module7

Filter door8

Amersham Typhoon Operating Instructions 29193226 AC 43

1

2

4

3

3 System description

3.2 Illustrations

Rear and left view of the
Amersham Typhoon scanner

FunctionPart

Air intake filter and fan1

Exhaust outlet2

Exhaust outlet3

USB connector4

44 Amersham Typhoon Operating Instructions 29193226 AC

Instrument panel

1 2 3 4

3 System description

3.2 Illustrations

Function/statusColorPart

On/Off buttonWhite1

White LED light2

Blue LED light3

If steady: Instrument is ready

•

If flashing: Instrument is starting up or

•
shutting down

The instrument is scanning, or

•

The filters are being moved inside the

•
scanner (after filter installation).

ErrorRed LED light4

Amersham Typhoon Operating Instructions 29193226 AC 45

1

2

3 System description

3.2 Illustrations

Filter module and stage

FunctionPart

Filter module1

Stage2

46 Amersham Typhoon Operating Instructions 29193226 AC

3.3 Stages

About this section

This section describes the different stages and accessories that are supplied with the
Amersham Typhoon scanner.

In this section

3 System description

3.3 Stages

See pageSection

483.3.1 Overview of stages

493.3.2 Fluor stage with accessories

533.3.3 Phosphor stage with accessories

553.3.4 Multi stage with accessories

Amersham Typhoon Operating Instructions 29193226 AC 47

3 System description

3.3 Stages

3.3.1 Overview of stages

3.3.1 Overview of stages

Stages

Three types of stages can be used with the Amersham Typhoon scanner depending on
the type of scanning:

•

Fluor stage

•

Phosphor stage

•

Multi stage

Accessories

The table below lists the different stages and accessories, and for which applications
the stages and accessories are used.

ApplicationAccessoryStage

stage

Note:

Fluores­cence

XMembrane weightFluor stage

XTiter plate holderMulti stage

XGlass slide holder

XGlass plate guide (33 × 42

cm)

The magnetic phosphor stage is used for standard storage phosphor screens.
In addition, the fluor stage can be used to scan unmounted phosphor screens,
and the multi stage can be used to scan mounted phosphor screens.

Phosphor
Imaging

XStorage phosphor screenPhosphor

XCassette

XSuction rod

Densitome­try

XDigitization plate

XXSpacers

48 Amersham Typhoon Operating Instructions 29193226 AC

3.3.2 Fluor stage with accessories

Illustration of the fluor stage

The fluor stage is used for scanning gels, membranes or other types of samplesthat are
placed directly on the stage. The fluor stage has a glass surface.

3 System description

3.3 Stages

3.3.2 Fluor stage with accessories

Digitization plate

The digitizationplate isa fluorescentplate thatemits lightwhen exposedto a laser beam.
The digitization plate is used together with spacers for densitometry (measurement of
relative optical density) and digitization of samples.

Amersham Typhoon Operating Instructions 29193226 AC 49

3 System description

3.3 Stages

3.3.2 Fluor stage with accessories

Spacers

There are two types of spacers that can be used with the fluor stage:

FunctionSpacer type

Sample spacer (single
spacer)

Digitization/OD spacers
(pair of spacers)

The image below shows the sample spacer.

The digitization/OD spacers are used together with the digitization plate for digitization
of samples and for measurements of relative OD in the densitometry scan mode. The
digitization plate rests on the spacers, so that there is space for the sample between the
fluor stage and the digitization plate. The illustration below shows the digitization/OD
spacers.

The digitization/ODspacers canbe usedboth for digitization and for OD measurements,
depending onthe orientation and placement of the spacers. The illustationbelow shows
the orientation in which the spacers are used for digitization.

Used toavoid thatthe sampleis placedoutside thescan
area

Used to hold the digitization plate in position, and to
create space for the sample between the digitization
plate and the fluor stage.

50 Amersham Typhoon Operating Instructions 29193226 AC

The illustration below shows the orientation in which the spacers are used for OD mea-

1

1

2

3

4

surements.

The spacers are placed by hooking them onto grooves on the fluor stage. Refer to

Spacer positioning, on page 51 for more information about positioning the spacers.

Spacer positioning

The spacers are placed and secured on the fluor stage by hooking the spacers onto
grooves on the fluor stage. The following illustration shows the positions of the grooves
on the fluor stage.

3 System description

3.3 Stages

3.3.2 Fluor stage with accessories

Amersham Typhoon Operating Instructions 29193226 AC 51

3 System description

3.3 Stages

3.3.2 Fluor stage with accessories

(pair)

For digitization, the spacers should be placed with the digitization label facing up, and
the arrows pointing to the scan area. For OD measurements, the spacers should be
placed with the OD label facing up.

Membrane weight

The membrane weight is used to press fluorescent membranes flat against the fluor
stage. The use of a membrane weight improves image quality, since the membrane
weight removes air bubbles and reduces vibration of the membrane during scanning.

PositionType of spacer

Placed between groove 1 and 2Sample spacer

Placed between groove 1 and 2, and between 3 and 4.Digitization/OD spacers

52 Amersham Typhoon Operating Instructions 29193226 AC

3.3.3 Phosphor stage with accessories

Phosphor stage

The phosphorstage is used for scanning storage phosphorscreens. Thephosphor stage
is magnetic so that it holds the storage phosphor screen in place.

3 System description

3.3 Stages

3.3.3 Phosphor stage with accessories

Storage phosphor screen

Storage phosphor screens, or phosphor imaging plates (IP), can be scanned by the
Amersham Typhoonscanner to capturetwo-dimensional imagesof radioactively labeled
samples.

A phosphor storage screen contains a layer of a polyester base material that is densely
coated withfine photo-stimulablecrystals, forexample BaFBrdoped withtraces of Eu2+.
When the storage phosphor screen is exposed to radioactively labeled samples, the
energy of the ionizing radiation is absorbed in the crystals. The accumulated radiation
energy can subsequently be detected by the Amersham Typhoon scanner. The surface
of the storage phosphor screen is scanned with a laser inside the scanner, and the ex­posed areas emit light, which is detected by the photomultiplier tube (PMT). In this way,
the Amersham Typhoon scanner creates a digital image of the radiated areas of the
storage phosphor screen.

Storage phosphorscreens are exposedto radioisotope-labeledsamples byplacing them
in close contact with the samples in an X-ray film-like cassette.

After scanning, the storage phosphor screens can be erased using Amersham Eraser,
so that they can be re-used.

Amersham Typhoon Operating Instructions 29193226 AC 53

3 System description

3.3 Stages

3.3.3 Phosphor stage with accessories

Cassette

Exposure cassettes are used for exposing storage phosphor screens to radioactively
labeled samples. The time of exposure varies depending on the samples.

Suction rod

The suction rod is used to lift up or place the storage phosphor screen on the phosphor
stage.

54 Amersham Typhoon Operating Instructions 29193226 AC

3.3.4 Multi stage with accessories

1

9

8

7

6

5

4

3

2

Multi stage

The multi stage is used to scan titer plates, glass slides or gels in glass cassettes.

3 System description

3.3 Stages

3.3.4 Multi stage with accessories

FunctionPart

Guide plate position lock screw1

Groove for titer plate holder2

Groove for DIGE gel glass cassette3

6

Screw to secure spring lock4

Spring lock to hold glass cassette or titer plate holder5

Height adjustment ridges for positioning the titer plate holder and glass
cassettes (3 mm and 5 mm)

Movable guide plate7

Lock screw, holds the movable guide plate on the multi stage8

Movable guide plate9

Amersham Typhoon Operating Instructions 29193226 AC 55

3 System description

3.3 Stages

3.3.4 Multi stage with accessories

Titer plate holder

The titer plate holder is used when scanning titer plate samples. The titer plate holder is
placed on the multi stage using guide plates. Up to nine titer plates can be analyzed in
one scan.

Glass slide holder (optional)

The glass slide holder is used to place glass slides on the multi stage for scanning. The
glass slide holder is used together with the titer plate holder.

Glass plate guides (optional)

For large glass cassettes (max. 33 × 42 cm), a pair of glass plate guides is available that
keep the glass cassette in place on the multi stage. The glass plate guides are used in­stead of the default movable guide plates. The thickness of each glass in the glass cas­sette should be 5 mm.

56 Amersham Typhoon Operating Instructions 29193226 AC

3.4 Filters

Filters in the Amersham Typhoon
scanner

Depending on the system configuration, different filters are installed in the
Amersham Typhoon scanner. The installed filters appear in the Instrument status pane
in Amersham Typhoon Control Software, see Instrument status, on page 64. All standard
filters are high performance band-pass filters.

Additional filters

It is possible to install additional filters, for example long-pass filters. These accessory
filters are delivered mounted in filter holders which are recognized by the
Amersham Typhoonscanner. Contact your local GE representative forinformation about
the available filters.

3 System description

3.4 Filters

Custom filters

It is also possible to use custom (third party) filters. Amersham Typhoon scanner is sup­plied with two accessory custom filter holders in which these custom filters can be
mounted. These filters are recognized by the scanner as Custom1 and Custom2 filters.

For information on how to mount a custom filter into the custom filter holder, refer to

Section 6.1.3 Custom filters, on page 101.

Amersham Typhoon Operating Instructions 29193226 AC 57

4 Amersham Typhoon Control Software

4 Amersham Typhoon Control

Software

About this chapter

This chapter describes the user interface of the Amersham Typhoon Control Software.

In this chapter

See pageSection

594.1 Main window

664.2 Scanning modes

694.3 General settings

724.4 Scan area functions

764.5 Image file settings

784.6 View Image mode

58 Amersham Typhoon Operating Instructions 29193226 AC

4.1 Main window

1

2

4

3

5

6

8

7

9

Introduction

This section gives an overview of the main window and describes the settings that can
be made.

Example of the main window

When the Amersham Typhoon Control Software is started, the main window appears.
The main window contains the following panes and fields.

4 Amersham Typhoon Control Software

4.1 Main window

Reference/DescriptionFunctionPart

See Scanning mode tabs, on page 60Scanning mode tabs1

See Scan settings, on page 60Scan settings2

See Stage/Area settings, on page 62Stage/Area settings3

See Image file settings, on page 63Image file settings4

See Method and scanning, on page 63Method and scanning5

Amersham Typhoon Operating Instructions 29193226 AC 59

4 Amersham Typhoon Control Software

4.1 Main window

Reference/DescriptionFunctionPart

See Instrument status, on page 64Instrument status6

See View Image, on page 65View image7

Scanning mode tabs

The scanning mode can be chosen by clicking on one of the scanning mode tabs.

Depending on the chosen scanning mode, different options will be available in the Scan
settings pane.For examplesof the different scanningmode windows,refer toSection 4.2

Scanning modes, on page 66.

General settings8

FunctionScanning mode

Opens the fluorescence imaging windowFluorescence

Opens the phosphor imaging windowPhosphor Imaging

Opens the densitometry scanning windowDensitometry

See Section 4.3 General settings, on

page 69

Help button9

About the Amersham Typhoon Control
Software

Scan settings

Depending onthe scanningmode, different scansettings canbe chosen.All scansettings
are saved in a Method. There are several pre-programmed methods to choose from
when starting an experiment. If a method is edited by changing any parameter setting,
the user can save the new method with a new method name.

For more information about scan settings in the different scanning modes, refer to Sec-

tion 4.2 Scanning modes, on page 66.

60 Amersham Typhoon Operating Instructions 29193226 AC

4 Amersham Typhoon Control Software

scanning.

Note:

In the Fluorescence mode, up to five sequen­tial scans can be performed in one method.

A sample with a smaller pixel size can be
analyzed in more detail, but the scanning
time and the image file size will increase.

tube (PMT).

The higher the PMT voltage, the higher the
sensitivity. However, a too high PMT voltage
may lead to image saturation and a high
background. It is therefore recommended
to optimize PMT voltage for each sample
type.

4.1 Main window

Scanning modeDescriptionScan setting

1

F

2

P

3

D

XXXSelects a pre-programmed method for

4

XXSelects the pixel size for scanning.

X

XXSelects the voltage of the photo-multiplier

XSets the voltage of the PMT automatically

based on a pre-scan.

XSelects the scan speed: Normal or Slow.

Normal scan speed is recommended for
most applications.

To reduce background noise, use a slow
scan speed.A slowscan speedreduces noise
by data averaging.

XAdds additional scans.

XChanges betweenscan areas (DIGEmethod).

XAdds an annotation (DIGE method).

An annotationcan be deleted with the small
cross.

1

Fluorescence mode

2

Phosphor Imaging mode

3

Densitometry mode

Amersham Typhoon Operating Instructions 29193226 AC 61

2

1

4 Amersham Typhoon Control Software

4.1 Main window

4

Only for digitization

Stage/Area settings

In the Stage/Area pane, the area that is to be scanned is defined.

FunctionNamePart

Stage/Area1

Scanning surface2

For more information about the Stage/Area settings, refer to Section 4.4 Scan area

functions, on page 72.

62 Amersham Typhoon Operating Instructions 29193226 AC

Selects the stage and area that will be used
for scanning.

Displays the total scanning surface of the
scanner:

White areaindicates anarea that will not

•
be scanned.

Blue area indicates a scan area that will

•
be scanned.

Blue frame (white area) indicates a scan

•
area that has been disabled. A disabled
scan area will be omitted during scan­ning.

Image file settings

In the image file settings pane, the image file settings can be defined.

4 Amersham Typhoon Control Software

4.1 Main window

FunctionField

Displays the folder where the image file will be saved.Image folder

Specifies the folder where the image file will be saved.Browse

File name

Note

For more information about image file settings, refer to Section 4.5 Image file settings,

on page 76.

Method and scanning

Specifies the image file name.

Note:

Details of the used laser and filter combination and the area
name (A, B, C, ...) are automatically added to the file name when
the file is saved. If the file name is not specified, it is automatically
set to date and time.

Selects the image file format.Format(s)

An optional note can be entered. The note is saved with the
image file, and can be viewed with the ImageQuant analyzing
software.

Displays the estimated scanning time.Scan time

Displays the total file size of the image files after scanning.Total file size

FunctionButton

Opens the Input method name dialog box to save the methodSave Method

Deletes the methodDelete Method

Starts a pre-scanPre Scan

Starts a scanScan

Amersham Typhoon Operating Instructions 29193226 AC 63

4 Amersham Typhoon Control Software

4.1 Main window

Instrument status

The instrument status pane shows the status of the lasers, filters and photo-multiplier
tubes (PMT).

FunctionField

List of installed lasersLaser

List of installed filtersFilter

List of photo-multiplier tubesPMT

Status

For moreinformation about the installed laser,refer to Laser specifications, on page132.
For more information about the filters, refer to Section 3.4 Filters, on page 57. For more
information about the photo-multiplier tubes,refer to Photomultiplier tubes, on page 41.

Displays thecurrent status of the Amersham Typhoon scanner.

The status of the scanner can be one of the following:

Scanner not connected

•

Initializing

•

Ready

•

Pre-scanning

•

Pre-scan(s) completed

•

Scanning

•

Scan(s) completed and image(s) saved

•

Filter moving

•

Pre-scan(s) stopped

•

Scan(s) stopped and image saved

•

Scan(s) stopped and image not saved

•

64 Amersham Typhoon Operating Instructions 29193226 AC

View Image

4 Amersham Typhoon Control Software

4.1 Main window

FunctionButton

View Image

Refer to Section 4.6 View Image mode, on page 78 for more information about the View
Image mode.

Displays the image in the View Image mode after a pre-scan
or a scan.

Amersham Typhoon Operating Instructions 29193226 AC 65

4 Amersham Typhoon Control Software

4.2 Scanning modes

4.2 Scanning modes

Fluorescence imaging tab

The following default methods can be selected on the Fluorescence tab:

1

Single channel fluorescence scan for all available lasers.

2

DIGE three-color muliplexing method.

3

Dark scan forluminescent samples. The default dark scan method uses the bi-alkali
PMT, and does not use any lasers or filters.

The scan settings and Stage/Area area settings can be edited in a default method. An
edited method can be saved under a new name for re-use at a later time.

The following illustration shows and example of a DIGE method set up screen.

66 Amersham Typhoon Operating Instructions 29193226 AC

Phosphor imaging tab

The following illustration shows an example of a Phosphor Imaging set up screen.

4 Amersham Typhoon Control Software

4.2 Scanning modes

Amersham Typhoon Operating Instructions 29193226 AC 67

4 Amersham Typhoon Control Software

4.2 Scanning modes

Densitometry tab

The following default methods can be selected on the Densitometry imaging tab:

1

OD method for measurements of relative optical density

2

Digitization method blue (blue laser)

3

Digitization method green (green laser)

The following illustration shows an example of the OD method set up screen.

68 Amersham Typhoon Operating Instructions 29193226 AC

4.3 General settings

Introduction

This section gives a detailed description of the general settings dialog. The General set­tings dialog is used to set laser and filtercombinations, setauto correction, selectdisplay

scale, and generate service log files.

Laser and filter combinations

Amersham Typhoon scanner comes with a number of default laser and filter combina­tions. New laser, filter, and detector combinations can be created on the Laser & Filter
tab in the General settings dialog.

4 Amersham Typhoon Control Software

4.3 General settings

FunctionButton

Deletes a laser and filter combination.Delete

Amersham Typhoon Operating Instructions 29193226 AC 69

4 Amersham Typhoon Control Software

4.3 General settings

FunctionButton

Edit a laser and filter combination.Edit

The following dialog opens:

Add a new laser and filter combinationAdd

Note:

Note:

Auto correction

Amersham Typhoonscanner is installed with correctionfiles. Itis recommendedto always
use the correction files (auto correction on). The auto correction can be turned off in the
General settings dialog on the Auto correction tab.

The default laser and filter combinations cannot be edited or removed.

For fluorescence scanning, only the multi-alkali PMT can be used.

70 Amersham Typhoon Operating Instructions 29193226 AC

Display scale

4 Amersham Typhoon Control Software

4.3 General settings

In the General settings dialog on the Display scale tab, it is possible to select display
scale for Phosphor Imaging. This selection only affects how images are displayed, it
does not affect the saved image.

Service log

In the General settings dialog on the Service tab, a log file can be generated which is
used for instrument service or troubleshooting.

Amersham Typhoon Operating Instructions 29193226 AC 71

4 Amersham Typhoon Control Software

4.4 Scan area functions

4.4 Scan area functions

Introduction

This section gives a detailed description of the Stage/Area settings.

Defining the scan area

In the Stage/Area pane, the area that is to be scanned can be defined.

ActionIf you want to...

Click anddrag tocreate anew scanarea.Add a new scan area

Delete a scan area

72 Amersham Typhoon Operating Instructions 29193226 AC

Select thescan areaand press theDelete
key on the computer keyboard.

Click and drag the blue box.Change the position of a scan area

Click and drag the edges of the blue box.Change the size of a scan area

Additional scan area functions

A menu containing additional scan area functions opens when you right-click on ascan
area.

4 Amersham Typhoon Control Software

4.4 Scan area functions

FunctionButton

Enables or disables an individual scan area.Enabled

A tick mark indicates that the scan area is enabled and will be
included in the scan.

Note:

A disabled scan area will be omitted during scanning.

Setting...

Duplicates the selected scan area.Duplicate

Assigns a new name to the scan area (A, B, C...).Reorder

When the name of one scan area is changed, all other scan
areas will be renamed as well.

Deletes the scan area.Remove...

Opens the dialog box Scan Area Settings, where the size and
position of the scan area can be adjusted.

Amersham Typhoon Operating Instructions 29193226 AC 73

4 Amersham Typhoon Control Software

4.4 Scan area functions

Image Area Settings dialog

To open the Image Area Settings dialog, right-click on a scan area and select Setting....

Grid

Millimeter

FunctionField

Defines the scan area based on the grid on the stage. The
maximum and minimum values are displayed above the list
boxes.

Lower Left sets the position of the lower left corner of the

•
scan area.

Upper Right sets the position of the upper right corner of

•
the scan area.

Defines thescan areabased onthe positionon thestage, based
on the distance from the front left corner of the total scanning
surface (mm).The maximumand minimum valuesare displayed
above the list boxes.

Lower Left sets the position of the lower left corner of the

•
scan area.

Upper Right sets the position of the upper right corner of

•
the scan area.

Assigns a name (A, B, C, ...) to the scan area.Area

When the name of one scan area is changed, all other scan
areas will be renamed as well.

74 Amersham Typhoon Operating Instructions 29193226 AC

4 Amersham Typhoon Control Software

4.4 Scan area functions

FunctionField

Enables or disables an individual scan area.Enabled

A tick mark indicates that the scan area is enabled and will be
included in the scan.

Note:

A disabled scan area will be omitted during scanning.

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4 Amersham Typhoon Control Software

4.5 Image file settings

4.5 Image file settings

Available image file formats

Depending onthe scanningmode, theimages canbe savedin .gel, .img, or .tif file format.

FluorescenceFile

format

Description of image file formats

In the fluorescence and phosphor imaging modes, the output log data from the PMT
detectors is first converted to a linear 32-bit floating decimal data format. This data is
stored temporarily for generating 16-bit .img, .gel, and .tif files. Different conversion
functions are used for the different file formats:

•

The .img format uses a logarithmic compression of data which preserves the many
intensity levels of the original data, especially in the low-intensity region. This allows
for measurements overa broaddynamic range.Information aboutthe scanis stored
in a separate text file.

•

The .gelformat usesa squareroot algorithmto accuratelyrepresentsmall differences
in data, whereas a .tif format may assign the same number to two values, particu­larly in the fainter part of the image. Image files include information about scan
settings, for example date andtime, which canbe viewedafter the scan,for example
using ImageQuant.

In addition to the .img and .gel formats, the .tif format can be used for presentation of
fluorescence and phosphor imaging data.

In thedensitometry mode,the .tifformat isused for saving digitizationimages andrelative
OD scans.

DensitometryPhosphor

Imaging

Digitiz.ODDIGEGeneral

N/AN/AXXX.gel

N/AN/AXN/AX.img

XXXN/AX.tif

76 Amersham Typhoon Operating Instructions 29193226 AC

Choosing an image file format

The choice of file format depends on the purpose of the scan and the analysis software.

In the fluorescence and phosphor imaging mode, the .img, .gel and .tif formats are
available.

•

For quantitative analysis use the .img format or the .gel file format.

•

For DIGE applications, and when image properties need to be read in IQTL, use the
.gel format.

In the Densitometry mode, the data is automatically converted and saved as a .tif file.

4 Amersham Typhoon Control Software

4.5 Image file settings

Amersham Typhoon Operating Instructions 29193226 AC 77

1

2

3

4

5

4 Amersham Typhoon Control Software

4.6 View Image mode

4.6 View Image mode

View Image window

During and aftera pre-scan ora scan, theimage can be viewed inthe View Image mode.
The ViewImage window is automaticallyopened duringa scan.The followingillustration
gives an example of the View Image window and its possible functions. The actual
functions displayed inthe ViewImage window will varydepending onwhether theimage
is viewed during or after a pre-scan or a scan.

AvailabilityFunctionPart

1

78 Amersham Typhoon Operating Instructions 29193226 AC

2

the PMT.

the area and calculate an estimated PMT that gives
a non-saturated image.

simulate images using different PMT settings.

Pre-scan and scanClick to select the scan that is displayed or adjust

Pre-scanIn the Pre-scan tab of the Adjust PMT field, select

Pre-scanUse the Estimated tab of the Adjust PMT field to

Pre-scan and scanScanned image.3

4 Amersham Typhoon Control Software

4.6 View Image mode

AvailabilityFunctionPart

4

5

Image display settings

on page 79.

Scan to start a scan.

port, print and save images.

FunctionButton

Selects themagnification (ratio)at whichthe imageis displayed.

Zooms in and out of the displayed image.

Selects thecolors thatare used to display theimage, forexam­ple:

Negative Gray

Pre-scan and scanImage display settings, see Image display settings,

Pre-scanClick Back to return to the previous window or click

ScanExport Display, Print, and Save as functions to ex-

Black and white display of intensity
levels based on a gray-scale.

Multi Color

Selects the type of tone curve used in the intensity graph.

Exponential

Linear

Sigmoid

Tip:

It is recommended to use the linear curve setting

Amersham Typhoon Operating Instructions 29193226 AC 79

Choose thisoption after a scan toview
multiple scans in different colors.

The lightintensities aredisplayed using
an exponential tone curve.

The lightintensities aredisplayed using
a linear tone curve.

The lightintensities aredisplayed using
a sigmoid (S-shaped) tone curve.

4 Amersham Typhoon Control Software

4.6 View Image mode

FunctionButton

Automatically adjuststhe rangeof lightintensities for optimum
display.

Intensity graph: displays the intensity levels of the image, and
can be used to adjust the contrast in the image.

The Low and High values below the graph correspond to the
range of intensities displayed. These values are displayed in
the graph as vertical red lines. To display a larger or smaller
interval of intensities, drag the red lines to adjust the Low and
High values, or type the limits directly in the Low and High
boxes.

80 Amersham Typhoon Operating Instructions 29193226 AC

5 Installation

About this chapter

This chapter provides required information to enable users and service personnel to
unpack, install, and transport Amersham Typhoon scanner.

CAUTION

Only authorized service personnel are allowed to install or move
Amersham Typhoon scanner.Contact your localGE representative
for help and advice.

In this chapter

This chapter contains the following sections:

5 Installation

See pageSection

825.1 Site requirements

855.2 Delivery and transport

865.3 Setup

885.4 Connections

Amersham Typhoon Operating Instructions 29193226 AC 81

5 Installation

5.1 Site requirements

5.1 Site requirements

Precautions

CAUTION

Install the Amersham Typhoon scanner in a location where it will
not come into contact with water or chemicals.

NOTICE

The Amersham Typhoon is intended for indoor use only.

Space requirements

Required bench space area

Amersham Typhoon scanner

RequirementParameter

110 cm × 90 cm (including required free space at
the left, right and rear)

70 cm at the front of the instrumentFree space required around

10 cmat left, right, top and rear of the instrument

Stable, horizontal surface.Placement

Do not place in direct sunlight or in brightly lit
places.

82 Amersham Typhoon Operating Instructions 29193226 AC

Dimensions

90 cm

80 cm

40 cm

5 Installation

5.1 Site requirements

The following illustration shows the dimensions of Amersham Typhoon scanner.

Amersham Typhoon scanner weighs 90 kg.

Environmental requirements

environment

Electrical requirements

RequirementParameter

18°C to 28°CAmbienttemperature,operation

20% to 70%, non-condensingMax. relative humidity,operation

2000 m above sea levelMax. altitude, operation

Pollution Degree 2Pollution degree of the intended

RequirementParameter

100 to 240 V AC ±10%Supply voltage

50 to 60 HzFrequency

2.5 to 1.0 AMax current

Overvoltage category IITransient overvoltages

Amersham Typhoon Operating Instructions 29193226 AC 83

5 Installation

5.1 Site requirements

Computer requirements

Minimum requirementParameter

Operating system

Microsoft®Windows®7 Professional SP1 (64-bit) En­glish/Japanese

or

Microsoft Windows 8.1 Pro (64-bit) English/Japanese

or

Microsoft Windows 10 Pro (64-bit) English/Japanese

8 GBInternal memory

Intel®Core i5 processorProcessor

80 GBFree space on hard drive

One USB 2.0 portOther requirements

DVD-ROM drive

NOTICE

The user is responsible for avoiding unauthorized use of the com­puter that is connected to the scanner.

NOTICE

The automatic sleep mode of the computer should be turned off
to prevent the computer from entering sleep mode during a scan.
Otherwise loss of data might occur.

Note:

84 Amersham Typhoon Operating Instructions 29193226 AC

At installation, the GE service engineer will turn off the automatic sleep mode
of the computer.

5.2 Delivery and transport

Upon delivery

Upon receiving Amersham Typhoon scanner, inspect the package for external damage
and check that all items in the packing list are included.

Should you find any external damage, or if any items on the packing list are missing,
notify the delivery company and contact GE for further advice.

Storing the Amersham Typhoon
scanner

Store Amersham Typhoon scanner in an environment according to the requirements in

Section 5.1 Site requirements, on page 82 until the product is unpacked and installed.

Moving the Amersham Typhoon
scanner

5 Installation

5.2 Delivery and transport

CAUTION

Turn off the power switch and remove connecting cables before
moving the equipment.

If you need to transport the Amersham Typhoon scanner, contact your local GE repre­sentative for help and advice. See also Removal from the controlled area, on page 18.

Amersham Typhoon Operating Instructions 29193226 AC 85

5 Installation

5.3 Setup

5.3 Setup

Precautions

WARNING

Do not block the ventilation inlets or outlets on the system.

WARNING

Do not block access to the power switch and power cord. The
power switch must always be easy to access. The power cord with
plug must always be easy to disconnect.

WARNING

Do not place the equipment on unstable tables or on inclined sur­faces, asthe equipment couldbe droppedor fall, resultingin injury.

CAUTION

Do not block the cooling fans. If they are blocked, the instrument
may malfunction.

Place the scanner

Place the Amersham Typhoon scanner on a sturdy, horizontal surface. Make sure that
the following requirements are met when placing the Amersham Typhoon scanner.

•

The airintake fanon therear sideof theAmersham Typhoon scanneris unobstructed.

•

The air exhaust outlets on the right and rear of the Amersham Typhoonscanner are
unobstructed.

•

There issufficient free spacearound thescanner, see Space requirements, on page 82.

Do not place Amersham Typhoon scanner in direct sunlight, or in a brightly lit place.

If theinstrument is to be used for phosphorimaging, refer to Installation site of instrument,

on page 18 for additional requirements for the installation site.

86 Amersham Typhoon Operating Instructions 29193226 AC

When the scanner is in place

When the scanner is in place, the Amersham Eraser and Amersham Cabinet can be
placed to the left of the scanner, in such a way that the fronts of the instruments are
aligned.

NOTICE

Do not place the Amersham Eraser and Amersham Cabinet to the
right of the scanner, because then they block the access to the
power switch and power outlet.

5 Installation

5.3 Setup

Amersham Typhoon Operating Instructions 29193226 AC 87

5 Installation

5.4 Connections

5.4 Connections

Electrical power

WARNING

Do not use the equipment with a power supply other than that
recommended. Fire and electric shock could result.

WARNING

The Amersham Typhoon scanner must always be connected to a
grounded power outlet.

WARNING

Connect thepower supplydirectly to a grounded wallpower outlet.
The useof extension cords ormultiple loads on one electrical outlet
could result in fire and electric shock.

WARNING

Only use power cords delivered or approved by GE.

CAUTION

Do notuse the samepower supplyas that of large equipmentsuch
as an air conditioner or centrifuge. Malfunction could result.

ActionStep

1

Amersham Typhoon scanner uses a special AC power cord. Only use power cords that
are specified in GE service manuals or by GE service personnel.

88 Amersham Typhoon Operating Instructions 29193226 AC

Plug in the power cord inthe power cord connector on Amersham Typhoon
scanner, and connect the power cord to a grounded wall outlet.

Communication

CAUTION

•

Do not connect any USB devices other than the
Amersham Typhoon scanner to the computer in which the
Amersham Typhoon Control Software is installed. Malfunction
could result.

•

Use only the Amersham Typhoon Control Software during
scanning.

NOTICE

Any computer used with the equipment must comply with EN/IEC
60950-1, andbe installedand usedaccording to themanufacturer's
instructions.

ActionStep

5 Installation

5.4 Connections

1

Connect a USB cable to the USB port on the rear side of the
Amersham Typhoon scanner.

Connect the other end of the USB cable to the USB port on the computer.2

Amersham Typhoon Operating Instructions 29193226 AC 89

5 Installation

5.4 Connections

Installing Amersham Typhoon
Control Software

All tasks related to software installation require a computer account with administrator
privileges.

90 Amersham Typhoon Operating Instructions 29193226 AC

6 Operation

About this chapter

This chapter contains the information required to operate the Amersham Typhoon
scanner.

In this chapter

6 Operation

See pageSection

936.1 Preparing the Amersham Typhoon scanner

1036.2 Preparing the sample

1176.3 Performing a scan

Precautions

1226.4 Procedures after a scan

WARNING

Do not use the instrumentwithin or near a sink, or in humid(above
70% RH)or dustyenvironments. Fire andelectric shockcould result.

WARNING

Do notuse theequipment ifsmoke, strangenoises orstrange odors
can be perceived, or if the equipment becomes unusually hot. This
may result in fire or electric shock.

Stop using the equipment immediately, turn off the power switch
and unplug the equipment from the power outlet. Contact your
local GE representative to request repair.

Amersham Typhoon Operating Instructions 29193226 AC 91

6 Operation

CAUTION

Always wear gloves, protective glasses and a lab coat or similar
when handling samples.

CAUTION

Do not turn off power during operation as this can cause loss of
data in the internal memory. Only turn off power in an emergency
situation.

NOTICE

With regards to third party patents related to sample preparation
etc., it is recommend that you consult a lawyer or patent attorney
regarding obtaining appropriate licenses.

NOTICE

The automatic sleep mode of the computer should be turned off
to prevent the computer from entering sleep mode during a scan.
Otherwise loss of data might occur.

92 Amersham Typhoon Operating Instructions 29193226 AC

6.1 Preparing the Amersham Typhoon scanner

6.1 Preparing the Amersham Typhoon scanner

About this section

This section describes how to prepare the Amersham Typhoon scanner for a scan.

In this section

6 Operation

See pageSection

946.1.1 Starting the system

976.1.2 Changing the filters

1016.1.3 Custom filters

Precautions

CAUTION

Do not insert a storage phosphor screen in Amersham Typhoon
before turning on the machine. If a storage phosphor screen is
detected during the self-diagnosis of the Amersham Typhoon, the
sensitivity of the storage phosphor screen may deteriorate and
reduce the quality of the scanned data.

NOTICE

Avoid anyshock orvibration tothe equipment, asthis maydamage
the equipment.

Amersham Typhoon Operating Instructions 29193226 AC 93

6 Operation

6.1 Preparing the Amersham Typhoon scanner

6.1.1 Starting the system

6.1.1 Starting the system

Start the Amersham Typhoon
scanner

CAUTION

Do not insert a storage phosphor screen in Amersham Typhoon
before turning on the machine. If a storage phosphor screen is
detected during the self-diagnosis of the Amersham Typhoon, the
sensitivity of the storage phosphor screen may deteriorate and
reduce the quality of the scanned data.

ActionStep

Check that there is no stage in the scanner.1

Switch thepower switchon theright side of the instrument to the "I" position.2

Push the On/Off button in the instrument panel.3

94 Amersham Typhoon Operating Instructions 29193226 AC

6 Operation

6.1 Preparing the Amersham Typhoon scanner

6.1.1 Starting the system

ActionStep

The following will occur:

The Ready LED flashes.

•

The instrument will make one beeping sound within 5 minutes after the

•
On/Off button is pushed.

The Ready LED is lit.

•

Result: Amersham Typhoon scanner is ready for operation.

Start the Amersham Typhoon
Control Software

ActionStep

1

Start Amersham Typhoon Control Software from the Start menu, or usethe
desktop shortcut.

The following window is displayed while the Amersham Typhoon Control
Software is launched.

Amersham Typhoon Operating Instructions 29193226 AC 95

6 Operation

6.1 Preparing the Amersham Typhoon scanner

6.1.1 Starting the system

ActionStep

When the Amersham Typhoon Control Software is ready, the main window
is displayed. See Example of the main window, on page 59.

The instrument status is displayed in the Status field, see also Instrument

status, on page 64.

96 Amersham Typhoon Operating Instructions 29193226 AC

6.1.2 Changing the filters

Introduction

The filters that are installed in the Amersham Typhoon scanner are listed in the Instru­ment status pane in the main windowof the Amersham Typhoon Control Software, see

also Instrument status, on page 64.

It is possible to exchange filters if necessary. However, some of the default filters cannot
be exchanged, these are the phosphor imaging filter and the Cy2, Cy3 and Cy5 band­pass filters. There are four additional positions in the filter module that can be used for
near IR filters, long pass filters, and custom filters. Follow the instructions below to ex­change filters.

Remove the filter module from
the scanner

ActionStep

6 Operation

6.1 Preparing the Amersham Typhoon scanner

6.1.2 Changing the filters

1

Make sure theAmersham Typhoon scanner is not scanning.The blue indica­tor LED on the instrument panel should be off.

Press the button on the filter door to open the filter door.2

Amersham Typhoon Operating Instructions 29193226 AC 97

6 Operation

6.1 Preparing the Amersham Typhoon scanner

6.1.2 Changing the filters

ActionStep

Pinch the metal tab on the filter module, and pull out the filter module.3

Replace filters in the filter
module

ActionStep

Slide the holder on the filter module to the left to open the filter module.1

98 Amersham Typhoon Operating Instructions 29193226 AC

6 Operation

6.1 Preparing the Amersham Typhoon scanner

6.1.2 Changing the filters

ActionStep

Pull out the filter from under the metal ridge, and remove the filter.2

Insert a new filter in the filter module as follows:3

Hook a new filter under the metal ridge on the rear of the filter module.

1

Lower the front of the filter.

2

4

Slide the holder on the filter module to the right to close the filter module.
Make sure that all filters are stably locked inside the filter module.

Amersham Typhoon Operating Instructions 29193226 AC 99

6 Operation

6.1 Preparing the Amersham Typhoon scanner

6.1.2 Changing the filters

Place the filter module in the
scanner

ActionStep

5

7

Insert the filter module in the filter moduleholder. A clicking sound indicates
that the filter holder is in the correct position.

Close the filter door.6

In the Amersham Typhoon Control Software, check the list of filters in the
Instrument status pane. Make sure that the newly installed filters are cor­rectly registered.

Note:
The filters are recognized automatically. Custom filters are designated Cus-

tom1 and Custom2 in the control software. In case the scanner does not

automatically recognizes a filter, or identifies a filter incorrectly, contact a GE
representative for service.

100 Amersham Typhoon Operating Instructions 29193226 AC