FUJIFILM FLA-7000 Operation Manual

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Operation Manual

Version 2.0

2006. Mar

Page 2

2006. Mar

Page 3

Contents

2006. Mar

Contents

Part 1 Preparations Before Use ...................................................................... 1

1. Starting the Image Reader ................................................................................................. 2

2. About the Main Window ..................................................................................................... 4

Part 2 Reading Imaging Plates ........................................................................ 5

1. Setting the Reading Conditions..........................................................................................6

2. Setting the IP on the IP Stage ............................................................................................ 8

3. Setting the IP Stage on the FLA-7000 ...............................................................................8

4. Starting Reading ................................................................................................................9

Part 3 Reading Fluorescent Samples ........................................................... 13

1. Setting the Reading Conditions........................................................................................14

2. Setting a Fluorescent Sample on the FLUOR Stage........................................................17

3. Setting the FLUOR Stage on the FLA-7000 ..................................................................... 17

4. Starting Reading ..............................................................................................................17

Part 4 Reading Digitized Samples ................................................................ 21

1. Setting the Reading Conditions........................................................................................22

2. Setting a Digitized Sample on the FLUOR Stage.............................................................25

3. Setting the FLUOR Stage on the FLA-7000 ..................................................................... 25

4. Starting Reading ..............................................................................................................25

Part 5 Lasers and Filters, Other Settings ..................................................... 29

1. Registering Laser and Filter Combinations (Method) ....................................................... 30

2. Filter Module Settings (Filter Module) ..............................................................................31

2a. Registering Filters in the Image Reader ............................................................................................ 31

2b. Saving the Four Types of Filter Combinations .................................................................................... 34

2c. Recalling Four Types of Filter Combinations ...................................................................................... 35

2d. Registering a New Filter Name .......................................................................................................... 35

3. Other Settings (Preference...) ..........................................................................................36

3a. Settings for Scanning (Scan Settings) ............................................................................................... 36

3b. Selecting the File Format for Saving and the Analyzing Software to Launch (Image File Settings) ... 38

Part 6 Installing and Uninstalling the Software........................................... 41

1. Installation (For Windows®) .............................................................................................. 42

1a. Installation of FUJI USB Control Driver.............................................................................................. 42

1b. Installation of FUJI USB Function Driver............................................................................................ 47

1c. Installation of Image Reader FLA-7000 Software ..............................................................................48

2. Uninstallation (For Windows®) ..........................................................................................49

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3. Installation (For MacintoshTM) ...........................................................................................50

4. Uninstallation (For MacintoshTM).......................................................................................50

Part 7 Troubleshooting ................................................................................... 51

1. Errors ............................................................................................................................... 52

2. Warnings ..........................................................................................................................52

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Part

Preparations Before Use

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2006. Mar

1. Starting the Image Reader

Caution

Please note that the GUI screens may change without notice.

Note1:

The Image Reader FLA-7000 software is available in two types: a Windows version, and a Macintosh version. Both versions have the same functions. This manual uses the screens of the Windows version. If you are using the Macintosh version, follow the instructions in this manual, except those of OS-related operations (such as starting and exiting the software).

Note2:

The following computers are compatible: OS: Windows XP Professional SP2 or later, Mac OS X 10.3 or later Memory: 512 MB Interface: USB 2.0

Precautions for Use

Do not connect any USB devices other than the FLA-7000 to the computer in which the Image Reader is installed. Otherwise, it may cause malfunction. During reading, do not use any USB devices other than the FLA7000 connected to the computer. If USB devices are used simultaneously, image data may be lost.

1-1 Turn on the FLA-7000 and peripheral devices.

Caution

If an Imaging Plate is inserted in the FLA-7000 before turning it on, its scanned data cannot be guaranteed. The sensitivity of the Imaging Plate may deteriorate, based on the self-diagnosis of the FLA-7000.

USB Connection

1-2 Turn on the computer.

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1. Starting the Image Reader

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1-3 Make sure that the FLA-7000 has completed the warm-up. (Only

the power lamp on the upper left panel on the front of the FLA7000 is lit up when the warm-up is completed.) Then, perform the procedures below.

For the Windows version: Start the Image Reader FLA-7000 software from the Startup menu, or use the shortcut key.

Shortcut Key

For the Macintosh version: Double-click the alias or the software to start the Image Reader FLA-7000 software.

Software

Note:

After starting the Image Reader, its condition is displayed in the Status area.

• When disconnected: Cannot recognize FLA-7000. Please check connection and power.

• During warm-up: FLA-7000 is in self-diagnosis. Please wait.

• When reading is possible: Ready

1-4 The main window of the Image Reader FLA-7000 software is

displayed.

Alias

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2. About the Main Window

IP Mode

Click when reading an imaging plate.

Fluorescence Mode

Click when reading fluorescent samples.

2. About the Main Window

Digitizing Mode

Click when performing digitizing.

Method

Click this button to register or erase combinations of lasers and filters.

Filter Module

Use this button to change or register the filter.

Preference...

Use this button to set the display menu, file format, or the Log/ Square Root of image data type.

Filter

The loaded filters are displayed.

Status

The status of the FLA-7000 is displayed.

Ready

The FLA-7000 is ready for scanning.

Laser

The conditions of the loaded laser units are displayed. In this example, there are four types of laser units loaded: LD 670 nm laser, LD 650 nm laser, LD 473 nm laser, LD 635 nm laser. All the laser units except for the LD 670 nm laser are ready for operation (OK). The condition of the LD 670 nm laser shows that it cannot be used (NG).

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Part

Reading Imaging Plates

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1. Setting the Reading Conditions

1-1 Click the button.

The Reader Settings window for the IP mode is displayed.

: Return to the main window from the IP mode.

1-2 Make these settings before reading IPs.

Refer to the following explanations of reading conditions when making settings.

Specify where to save the file after reading.

Click the button.

Input the name of a file for saving data of a read image.

You may not start reading unless you input a file name.

The comment is saved with the image as a file, and can be viewed

with the analyzing software. Input it if necessary.

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1. Setting the Reading Conditions

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Before starting reading, make sure that the LD 650 nm laser is shown and the IP filter is selected in the Setting field as shown below. In IP reading, the LD 650 nm laser and IP filter are selected automatically.

The laser used for reading is displayed.

The filter used for reading is displayed.

Note:

To use the IP mode, the LD 650nm laser must be loaded, and the IP filter must be set. If these conditions are not fulfilled, the Reader Settings window for the IP mode cannot be accessed.

: Click to select from three types of reading sensitivity, as

shown on the left.

S10000

Sensitivity: high

Sensitivity: low

S1000

: Set the pixel size for reading. Click to select from one of

the 4 types, as shown on the left. A sample with a smaller pixel size is analyzed more finely.

200 100 50 25 µm

Short Reading Time Long

Small Image File Size Large

: Specify the dynamic range. The dynamic range that can be

detected is bigger with L5 than with L4. If the signals of the sample are in the L4 range, the density gradation is represented more finely if L4 is selected.

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2. Setting the IP on the IP Stage/3. Setting the IP Stage on the FLA-7000

Estimated time until reading finishes.

Size of the file.

Drag and select the scanning area on the IP stage.

2. Setting the IP on the IP Stage

Note:

Select this to specify the reading area based on the 2.5 cm grid lines on the IP stage. Select this to specify the reading area arbitrarily. Select this to specify a reading area of 20 cm x 25 cm. Select this to specify a reading area of 20 cm x 40 cm.

: Use this button when saving the reading conditions in a file. You

may save reading conditions that are used frequently with this function and recall them later with .

: Use this button when recalling reading conditions saved with

Note:

When starting the Image Reader, the settings information from the previous session is displayed.

Set the IP on the IP stage. For instructions on setting the IP, see the Fluorescent Image Analyzing System FLA-7000 Operation Manual.

3. Setting the IP Stage on the FLA-7000

Set the IP stage on the FLA-7000. For instructions on setting the IP stage on the FLA-7000, see the Fluorescent Image Analyzing System FLA-7000 Operation Manual.

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4. Starting Reading

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4. Starting Reading

4-1 Click the button to start reading.

The scanned area is displayed in the real-time window, as shown

below.

The stage is read from the left towards the right.

Reading may be finished at any time before the whole scanning area has completed reading. Click the

button when you want to

finish reading.

Note1:

If you click Stop during reading, the part that has not been read yet will be saved as an image with a data value of 0 (light intensity of 0).

Note2:

When you click the button, reading itself is canceled. You cannot start reading again from the location where reading stopped.

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4. Starting Reading

4-2 When you want to change the display parameters of the real-time

window, refer to the explanations below.

: Select the type of tone curve from the pull-

down menu. Exponential: The exponential tone curve is

used to adjust gradations.

Linear: The linear tone curve is used

to adjust gradations.

Sigmoid: The sigmoid tone curve is

used to adjust gradations.

: Drag the adjuster.

You may adjust the density of the read image. Data of a lower light intensity that the line on the left (Low value) will be displayed as a completely white image, and data of a higher light intensity than the line on the right (High value) will be displayed as a completely black image.

: If Auto Range Scope is checked, the Image

Reader automatically corrects the optimum tone.

Note:

The Image Reader converts data read from samples to images that have an information of 65536 tones, with 0 being the value for white, and 65535 being the value for black. The tones are indicated by the horizontal axis of the tone curve graph.

: You may change the display area by

selecting a magnification ratio from the pull-down menu. In addition, after reading, if you click the display area after clicking the buttons, the clicked area can be enlarged or reduced.

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4. Starting Reading

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4-3 : Save the data with a different file name.

: Launch the registered analyzing software to

display the image.

4-4 To read a second IP continuously, carry out reading by following

the above procedures. Click the button to return to the first Reader Settings window. Do not open the stage door of the FLA-7000 until the stage has completely returned. If it is opened, close it immediately. When scanning finishes, the and buttons become active, but the button is grayed out until the stage has completely returned.

4-5 Finish reading.

Before turning off the power of the FLA-7000, shut down the Image Reader software.

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4. Starting Reading

Note:

If the photo-multipliers (PMT) are replaced with a multi-alkali PMT, the IP mode switches from that of the regular IP mode to a PMT voltage adjustment mode. The PMT voltage adjustment mode differs from the regular IP mode in that a sensitivity level that matches the scanned sample can be set independently. Replacement of the standard PMT with a multi-alkali PMT is done by a serviceman. For details, contact an authorized dealer.

The Sensitivity settings differ from that of the regular IP mode. The operation procedures for other settings are the same as those of the regular IP mode. Follow the operation procedures of the regular IP mode.

IP mode

PMT voltage adjustment mode

You may set the voltage to be applied to the PMT as an integral value within the predetermined range. The larger the value is, the higher the sensitivity, but noise will be greater.

Small

Low High

200V 1000V

Value

Sensitivity

Large

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Part

Reading Fluorescent Samples

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1. Setting the Reading Conditions

1-1 Click the button.

The Reader Settings window for the Fluorescence mode is displayed.

: Return to the main window from the Fluorescence mode.

1-2 Make these settings before reading fluorescent samples.

Refer to the following explanations of reading conditions when making settings.

Click the button and specify where to save the file after reading.

Note:

When scanning multiple times, the image data is saved in the folder with the name specified in File Name, which is automatically created in the specified location.

Input the name of a file for saving data of an image.

You may not start reading unless you input a file name.

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1. Setting the Reading Conditions

000- 635- 1

File name

Laser name

Scan number

2006. Mar

Note:

When the number of scans is between 2 and 4, the laser name and scan number are automatically added to the specified name.

Example: If reading is set with the LD 635 nm laser, LD 473 nm

laser, LD 635 nm laser, and LD 635 nm laser, with "test" as the file name, then the following files are created in the "test" folder. test-635-1 test-473 test-635-2 test-635-3

The comment is saved with the image as a file, and can be viewed

with the analyzing software. Input it if necessary.

: Scanning can be performed up to 4

times. The following items can be set.

Note:

: From the pull-down menu, select the Method

that corresponds with the sample. The selected laser and filter combination is displayed below.

: You may set the voltage to be applied to the

photo-multiplier tube (PMT) as an integral value within the predetermined range. The larger the value is, the higher the sensitivity.

200V 1000V

Small

Low High

Value

Sensitivity

Large

: Click the button to increase the number of scans,

and click the button to reduce the number of scans. Up to 4 scans can be performed.

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1. Setting the Reading Conditions

: Set the pixel size for reading. Click to select from one of

the 4 types, as shown on the left. A sample with a smaller pixel size is analyzed more finely.

200 100 50 25 µm

Short Reading Time Long

Small Image File Size Large

: Specify the dynamic range. The dynamic range that can be

detected is bigger with L5 than with L4. If the signals of the sample are in the L4 range, the density gradation is represented more finely if L4 is selected.

Estimated time until reading finishes.

Size of the file. Expressed as file size per test x number of scans.

Drag and select the scanning area on the FLUOR stage.

Note:

Select this to specify the reading area based on the 2. cm grid lines on the FLUOR stage.

Select this to specify the reading area arbitrarily.

Select this to specify the entire FLUOR stage as the reading area.

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2. Setting a Fluorescent Sample on the FLUOR Stage/3. Setting the FLUOR Stage on the FLA-7000/4. Starting Reading

2006. Mar

: Use this button when saving the reading conditions in a file. You

may save reading conditions that are used frequently with this function and recall them later with .

: Use this button when recalling reading conditions saved with

Note:

When starting the Image Reader, the settings information from the previous session is displayed.

2. Setting a Fluorescent

Sample on the FLUOR Stage

3. Setting the FLUOR Stage

on the FLA-7000

4. Starting Reading

Set the fluorescent sample on the FLUOR stage. For instructions on setting the fluorescent sample, see the Fluorescent Image Analyzing System FLA-7000 Operation Manual.

Set the FLUOR stage on the FLA-7000. For instructions on setting the FLUOR stage on the FLA-7000, see the Fluorescent Image Analyzing System FLA-7000 Operation Manual.

4-1 Click the button to start reading.

Displays the scan results of the 1st, 2nd, 3rd, and 4th scan, starting from the left. Click on the thumbnails to switch the display.

Displays information related to the contents of the currently displayed scan.

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4. Starting Reading

The scanned area is displayed in the real-time window, as shown below. The stage is read from the left towards the right.

Reading may be finished at any time before the whole scanning area has completed reading. Click the

button when

you want to finish reading.

Note1:

If you click Stop during reading, the part that has not been read yet will be saved as an image with a data value of 0 (light intensity of 0).

Note2:

When you click the button, reading itself is canceled. You cannot start reading again from the location where reading stopped.

4-2 When you want to change the display parameters of the real-time

window, refer to the explanations below and make settings.

: Select the type of tone curve from the pull-

down menu. Exponential: The exponential tone curve is

used to adjust gradations

Linear: The linear tone curve is used

to adjust gradations.

Sigmoid: The sigmoid tone curve is

used to adjust gradations.

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4. Starting Reading

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: Drag the adjuster.

You may adjust the density of the read image.

Data of a lower light intensity than the line on the left (Low value) will be displayed as a completely white image, and data of a higher light intensity than the line on the right (High value) will be displayed as a completely black image.

: If Auto Range Scope is checked, the Image

Reader automatically corrects the optimum tone.

Note:

: You may change the display area by selecting a

magnification ratio from the pull-down menu. In addition, after reading, if you click the display

area after click the buttons, the clicked area can be enlarged or reduced.

4-3 : Save the data with a different file name.

: Launch the registered analyzing software to display

the image.

4-4 To read another fluorescent sample continuously, carry out reading

by following the above procedures.

Click the button to return to the first Reader Settings window. Do not open the stage door of the FLA-7000 until the stage has completely returned. If it is opened, close it immediately. When scanning finishes, the and buttons become active, but the button is grayed out until the stage has completely returned.

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4. Starting Reading

4-5 Finish reading.

Before turning off the power of the FLA-7000, shut down the Image Reader software.

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Part

Reading Digitized Samples

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1. Setting the Reading Conditions

1-1 Click the button.

The Reader Settings window for the Digitizing mode is displayed.

: Return to the main window from the Digitizing mode.

1-2 Make these settings before reading digitized samples.

Refer to the following explanations of reading conditions when making settings.

Specify where to save the file after reading.

Click the button and specify where to save the file after

reading.

Input the name of a file for saving data of the image.

You may not start reading unless you input a file name.

The comment is saved with the image as a file, and can be viewed

with the analyzing software. Input it if necessary.

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1. Setting the Reading Conditions

2006. Mar

: Select the Method that corresponds

with the sample from the pull-down menu.

The Method to be used for reading is displayed.

The contents of the laser and filter combination to be used for reading are displayed.

Note:

To use the Digitizing mode, the LD 473 nm laser must be loaded and the Y520 filter must be registered, or the SHG 532 nm laser must be loaded and the O580 filter must be registered. If these conditions are not fulfilled, the Reader Condition Settings window for the Digitizing mode cannot be accessed.

: You may set the voltage to be applied to the photo-

multipliers (PMT) as an integral value within the predetermined range.

: Set the pixel size for reading. Click to select one of the 4

types, as shown on the left. A sample with a smaller pixel size will be analyzed more finely.

200 100 50 25 µm

Short Reading Time Long

Small Image File Size Large

: Specify the dynamic range. The dynamic range that can be

detected is bigger with L5 than with L4. If the signals of the sample are in the L4 range, the density gradation is represented more finely if L4 is selected.

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1. Setting the Reading Conditions

Estimated time until reading finishes. Size of the file.

Drag and select the scanning area on the FLUOR stage.

Note:

Select this to specify the reading area based on the 2.5 grid lines on the FLUOR stage.

Select this to specify the reading area arbitrarily.

Select this to set the entire FLUOR stage as the reading area.

: Use this button when saving the reading conditions in a file. You

may save reading conditions that are used frequently with this function, and recall them later with .

: Use this button when recalling reading conditions saved with

Note:

When starting the Image Reader, the settings information from the previous session is displayed.

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2. Setting a Digitized Sample on the FLUOR Stage/3. Setting the FLUOR Stage on the FLA-7000/4. Starting Reading

2006. Mar

2. Setting a Digitized Sample

on the FLUOR Stage

3. Setting the FLUOR Stage

on the FLA-7000

4. Starting Reading

After setting the digitized sample on the FLUOR stage, place the fluorescent plate for digitizing on top of it. For instructions on setting the digitize sample, see the Fluorescent Image Analyzing System FLA-7000 Operation Manual.

Set the FLUOR stage on the FLA-7000. For instructions on setting the FLUOR stage on the FLA-7000, see the Fluorescent Image Analyzing System FLA-7000 Operation Manual.

4-1 Click the button to start reading.

The scanned area is displayed in the real-time window, as shown below.

The stage is read from the left towards the right.

Reading may be finished any time before the whole scanning area has completed reading. Click the

button when you

want to finish reading.

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2006. Mar

4. Starting Reading

Note1:

If you click Stop during reading, the part that has not been read yet will be saved as an image with a data value of 0 (light intensity of 0).

Note2:

When you click the button, reading itself is canceled. You cannot start reading again from the location where reading stopped.

4-2 When you want to change the display parameters of the real-time

window, refer to the explanations below and make settings.

: Select the type of tone curve from the pull-

down menu. Exponential: The exponential tone curve is

used to adjust gradations

Linear: The linear tone curve is used

to adjust gradations.

Sigmoid: The sigmoid tone curve is

used to adjust gradations.

: Drag the adjuster.

You may adjust the density of the read image. Data that is lighter than the line on the left (Low value) will be displayed as a completely white image, and data that is darker than the line on the right (High value) will be displayed as a completely black image.

: If Auto Range Scope is checked, the Image

Reader automatically corrects the optimum tone.

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4. Starting Reading

2006. Mar

Note:

: You may change the display area by selecting a

magnification ratio from the pull-down menu. In addition, after reading, if you click the display area after click the buttons, the clicked area can be enlarged or reduced.

4-3 : Save the data with a different file name.

: Launch the registered analyzing software to display

the image.

4-4 To read another digitized sample continuously, carry out reading by

following the above procedures. Click the button to return to the first Reader Settings window. Do not open the stage door of the FLA-7000 until the stage has completely returned. If it is opened, close it immediately. When scanning finishes, the and buttons become active, but the button is grayed out until the stage has completely returned.

4-5 Finish reading.

Before turning off the power of the FLA-7000, shut down the Image Reader software.

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2006. Mar

Page 33

Part

Lasers and Filters, Other Settings

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2006. Mar

1. Registering Laser and Filter Combinations (Method)

Note:

Method settings are registered by a serviceman upon installation. Under normal circumstances, it is not necessary to register these settings.

You may register, change, and delete laser and filter combinations.

1-1 Click the button on the main window.

The following dialog box appears.

Names of the laser and filter combinations

Click this button to delete laser and filter combinations

1-2 Click the button.

The following dialog box appears.

However, combinations of lasers and filters that are not loaded cannot be selected in the Reader Condition screen.

Click this button to edit laser and filter combinations

Click this button to registe new laser and filter combinations

Registered laser and filter combinations

Name:

Input a name for the combination to be registered.

Laser :

Select the type of laser. (You may also select lasers that are not actually loaded.)

Filter :

Select the type of filter. (You may also select lasers that are not actually loaded.)

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2. Filter Module Settings (Filter Module)

2006. Mar

1-3 Input a name for the combination, select the type of laser and filter,

and click the button. The laser and filter combination is registered .

Note:

Click the and buttons to delete or change a registered Method. Methods initially registered as default cannot be deleted or edited. The Methods marked with [**] are the Methods that are initially set as default.

2. Filter Module Settings

(Filter Module)

2a. Registering Filters in the

Image Reader

1-4 Click the button.

Note:

Filter Module settings are registered by a serviceman upon installation. Under normal circumstances, it is not necessary to register these settings.

After exchanging the filters of the FLA-7000, you must register the exchanged filters in the Image Reader.

Note:

If filters are not registered in the Image Reader, they are not displayed in the Image Reader window, even if they are physically set in the FLA-7000.

The following explains the method for registering filters in the Image Reader when the [605DF40] is set in filter module position No.2 (second from the back).

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2006. Mar

Caution

When removing the filter module, make sure to press the

Filter Settings window. If the filter module is forcibly removed, the part where the filter comes in contact with the photo-multipliers (PMT) will become damaged.

2a-1 Click the button on the main window.

The following window appears and the filter module moves to a position where it can be taken out.

Use this button to set a filter in the Image Reader It is also possible to use the mouse to drag-and-drop

2. Filter Module Settings (Filter Module)

button and remove it after the window changes to the

Use this button to remove a filter in the Image Reader

List of filters currently registered in the software Filters marked with [**] are the filters that are initially set as default

Corresponds to the numbers (4,3,2,1) on the filter tray

Use this button when loading saved filter combinations

Use this button when saving filter combinations as a file

: Use this button to add a new filter to the filter list.

: Use this button to change the name or color of the displayed icon for the registered filter. Default filters ([**]) cannot be edited.

: Use this button to delete a registered filter. Default filters ([**]) cannot be deleted.

2a-2 Exchange the filter of the FLA-7000.

For instructions on exchanging the filter of the FLA-7000, see the Fluorescent Image Analyzing System FLA-7000 Operation Manual.

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2. Filter Module Settings (Filter Module)

2006. Mar

2a-3 Click filter position No. 2.

A red frame appears around the selected filter position.

2a-4 Select [605DF40] from the Filter List.

The selected item is highlighted in blue.

2a-5 Click the button.

It is also possible to drag-and-drop the selected filter to filter position No. 2.

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2. Filter Module Settings (Filter Module)

Filter position No. 2 changes to [605DF40].

Note:

Click the button to keep the filter position empty after removing a filter.

2b. Saving the Four Types of

Filter Combinations

2a-6 Click the button.

You may save four types of filter combinations that are currently displayed.

Note:

Exchanging the module and saving/recalling filter combinations can be managed more easily if each user has their own filter module.

2b-1 Click the button on the main window.

2b-2 Click the button.

The following dialog box appears.

Filter Module Name:

Input a name for the filter combination.

2b-3 After inputting a name for the filter combination, click the

button. The filter combation is saved.

2b-4 Click the button.

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2. Filter Module Settings (Filter Module)

2006. Mar

2c. Recalling Four Types of

Filter Combinations

You may recall four types of filter combinations that are currently registered.

2c-1 Click the button on the main window.

2c-2 Click the

button.

The following dialog box is displayed.

2c-3 Select the name of the filter combination you want to recall, and

click the button. The filter combination is recalled.

2d. Registering a New Filter

Name

2c-4 Click the button.

You may register a filter name.

2d-1 Click the button on the main window.

2d-2 Click the button.

The following dialog box appears.

Name:

Input a name for the filter.

Icon:

Select the color of the filter icon to be displayed in the software.

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3. Other Settings (Preference...)

2d-3 Select the filter name and icon color you want to register, and click

the button. The filter is newly registered.

Note:

To delete or edit the register filter name, select the filter name and click the or button. Methods initially registered as default cannot be deleted or edited.

3. Other Settings (Preference...)

3a. Settings for Scanning

(Scan Settings)

2d-4 Click the button.

These are settings that are carried out when specifying settings for reading samples. Depending on the reading mode, there may be some functions that cannot be used.

3a-1 Click the button on the main window.

3a-2 Click the tab.

The following dialog box appears.

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3. Other Settings (Preference...)

2006. Mar

3a-3 For each of the following items, refer to the explanations below and

click the radio button.

When detecting a very small sample amount, the Log format is more effective, because the low-density areas are converted to the gray scale more finely in the Log format. Log format is also recommended for reading gels stained with CBB or silver in the Digitizing mode.

The image will have a low background and clear differences in density. If there are large sample amounts in the IP, Fluorescence, or Digitizing modes, the Root format is more effective.

Note:

The following software versions are required for quantitative analysis of images read in the Root format. Root format files cannot be opened properly with older software versions. For Windows: Science Lab 2003 (Multi Gauge Ver.2.1, Colony Ver.1.1, L-Process Ver.2.1) or later versions For Macintosh: Science Lab 2003 (Image Gauge Ver.4.2, L-Process Ver.2.1) or later versions

Uses specific image shading correction data that was adjusted in accordance with each laser in the Fluorescence mode.

Correction settings will be added in the Fluorescence mode. You will be able to select from optional image shading correction data.

Note:

Registration of the correction settings list is performed by a serviceman. Please contact the dealer where you purchased the FLA-7000, or contact Fuji Photo Film.

Does not use ND filter for adjusting light intensity.

Uses ND filter for adjusting light intensity.

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3. Other Settings (Preference...)

If select Quick, reading time would become shorter. However, noise would stand out during reading. The reading time varies depending on the setting image size and the scanning area. In case of reading the whole area of FLUOR stage, the reading time are as follows:

< Standard Mode > 200um : 210sec, 100um : 210sec, 50um : 330sec, 25um : 450sec

< Quick Mode > 200um : 150sec, 100um : 150sec, 50um : 210sec, 25um : 330sec

3a-4 A function for supporting new stages in the future.

3a-5 Click the button.

3b. Selecting the File

Format for Saving and the Analyzing Software to Launch (Image File Settings)

These are settings for saving images. Depending on the scanning mode, there may be some functions that cannot be used.

3b-1 Click the button on the main window.

3b-2 Click the tab.

The following dialog box appears.

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3. Other Settings (Preference...)

2006. Mar

3b-3 Refer to the explanations below and click a radio button.

The standard file format of the Fuji Film BAS/FLA series. Each file is saved as a combination of an information file (inf file) and a raster file (img file). The Fuji Film Science Lab or Array Gauge may be used for analysis.

In combination with an img file and inf file, a read image can also be saved in TIFF file format. For TIFF files, image data type is always set to Linear format.

3b-4 Click the button, and select the specified analyzing software.

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Page 45

Part

Installing and Uninstalling the Software

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2006. Mar

1. Installation (For Windows®)

1a. Installation of FUJI USB

Control Driver

It installs in the following sequence.

1a. Installation of FUJI USB Control driver 1b. Installation of FUJI USB Function driver 1c. Installation of Image Reader FLA-7000 software

Note:

The computer and FLA-7000 should not be connected with a USB cable during the operation.

1a-1 Open the control panel and click “Printers and Other Hardware”. 1a-2 Click the “Add Hardware”.

1a-3 Click the “Next” button.

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1. Installation (For Windows®)

2006. Mar

1a-4 Select “Yes, I have already connected the hardware” and click the

“Next” button.

1a-5 Select “Add a new hardware device” and click the “Next” button.

1a-6 Select “Install the hardware that manually select from a list

[Advanced]” and click the “Next” button.

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1. Installation (For Windows®)

1a-7 Select “Show All Devices” and click the “Next” button.

1a-8 Click “Have Disk...” button.

1a-9 Click “Browse...” button.

1a-10 Set the folder in which a file is saved to FLA-7000 CD-ROM.

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1. Installation (For Windows®)

2006. Mar

1a-11 Open the FUJIUSB control folder.

1a-12 Select a DevMng.inf file and click the “Open” button.

1a-13 Click the “OK” button.

1a-14 Click the “Next” button.

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1. Installation (For Windows®)

1a-15 Click the “Next” button.

1a-16 Click the “Continue Anyway” button.

1a-17 Click the “Finish” button.

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1. Installation (For Windows®)

2006. Mar

1b. Installation of FUJI USB

Function Driver

1b-1 Connect the USB cable and turn ON the power switch of the FLA-

7000.

Note:

Perform this operation, or the personal computer may be reset.

1b-2 Click the “Next” button.

1b-3 Click the “Finish” button.

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1. Installation (For Windows®)

1c. Installation of

Image Reader FLA-7000 Software

1c-1 Insert the installation CD-ROM of FLA-7000.

1c-2 Click the setup icon.

1c-3 Click the “Next” button.

1c-4 Click the “Install” button.

1c-5 Click the “Finish” button.

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2. Uninstallation (For Windows®)

2006. Mar

2. Uninstallation

(For Windows®)

2-1 Open the control panel and select “Add or Remove program”.

2-2 Select Image Reader FLA-7000 and click the “Remove” button to be

deleted.

➟ A progress bar is displayed and uninstallation is started.

Note:

Connection files created after installation of the reader software are deleted. Correction files such as shading data is required to read by FLA-7000. They arelocated in Data folder of FLA-7000 folder. The files you wish to keep must be saved outside the FLA-7000 folder.

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3. Installation (For MacintoshTM) / 4. Uninstallation (For MacintoshTM)

3. Installation (For MacintoshTM)

Installation of FLA-7000

4. Uninstallation (For MacintoshTM)

To install Image Reader FLA-7000 software, follow the procedure described below.

< For Mac OS10.4 >

3-1 Double-click the “FLA-7000 Install CD” icon to open it. 3-2 Click the “FLA-7000.pkg” icon. 3-3 Click the “Continue” button on the “Introduction” screen. 3-4 Click the “Continue” button on the “Installation Destination” screen. 3-5 Click the “Upgrade” button on the “Installation Type” screen. 3-6 Provide the name and password of the administrator. 3-7 Click the “close” button on the “Finish Up” screen.

Move the FLA-7000 folder contained in the Application folder on the Machintosh HD to your Trash bin.

Note:

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Part

Troubleshooting

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2006. Mar

1. Errors/2. Warnings

1. Errors

2. Warnings

An error is the condition in which all of the reading modes of the FLA-7000 cannot be used. When a warning occurs, the indicator lamps make a sound (“Be-beep, bebeep, be-beep”).

An error message dialog box is displayed on the Image Reader screen displayed on the computer.

Please contact Fuji Photo Film with the 4-digit Error Code and the 8-digit number inside the parentheses.

The following are examples of warnings that are displayed as a message in the Image Reader window. If a displayed message includes instructions, please follow them.

Message Filter module has not been setup. Push the filter module button and set up the filter module.

The combination of the lasers and filters could be inappropriate. Check the lasers and filters.

Meaning and Countermeasure Meaning:

• A filter module has not been set.

• Press the filter module button, and set a filter module.

Countermeasure: Confirm that a filter module is set.

Meaning: There is a possibility that the laser and filter combination is inappropriate. Please check the laser and filter.

Countermeasure:

• Confirm the Methods registered. It is possible that the laser and filter combination is inappropriate.

• Confirm that the filter specified in the Image Reader is actually set.

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2. Warnings

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Laser error occurred. Use other lasers.

Failed to retrive picture data from PC. Check the PC setting.

Meaning:

• A laser error has occurred.

• Use a different laser.

Countermeasure:

• An error has occurred with the laser.

• If an error occurs even after

restarting the instrument, call a serviceman.

• It is possible to scan using a different laser.

Meaning

• There was a failure in reading the

image data from the computer.

• Check the computer environment.

Countermeasure:

• It is possible that the computer's

processing ability has degraded.

• Check the computer once, when

there are no devices connected to it and no software started.

PMT error occurred. Use low sensitivity for the setting.

The file name is already used. Close the SampleSetDoor or FilterChangeDoor.

Meaning:

• An overexposure error has

occurred.

• Scan with low sensitivity settings.

Countermeasure:

• Overexposure has been detected

outside of the scanning area.

• If an error occurs even after

restarting the instrument, call a serviceman.

Meaning: Close the sample set door or the filter change door.

Countermeasure: Close the sample set door or the filter change door.

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2. Warnings

Note:

If an error message is displayed, a serviceman should take the countermeasures to resolve the trouble. Please contact the dealer where you purchased the FLA-7000, or contact Fuji Photo Film.

Fuji Photo Film Co., Ltd. 2-26-30 Nishi Azabu, Minato-ku Tokyo, Japan 106-8620 TEL: +81-3-3406-2201 FAX: +81-3-3406-2158 e-mail: sghelp@fujifilm.co.jp

For technical questions, send an e-mail to the following address. e-mail: sginfo@fujifilm.co.jp.