Froilabo BM 3E Essential, BM 3E Evolution User Manual

BM 3E

Ultra-low temperature freezer

Models:

 Essential
 Evolution

User manual – Issue 0.1

Original manual – Iss. 0.1 User manual – BM 3E Freezer 1/ 44

This document was drawn up with the utmost care. However,
Froilabo may not be held responsible in the event of errors or
omissions. The same applies to any damages arising from the use
of the information contained in this manual.

FROILABO
Lyon - 5 avenue Lionel Terray
69330 Meyzieu
FRANCE

Original manual – Iss. 0.1 User manual – BM 3E Freezer 2/ 44

1. Notes

All freezers and electronic equipment must be earthed. This protects users and the equipment.
Only operate a freezer after implementing all required safety measures.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 3/ 44

2. Symbols used in this manual

Information: This symbol indicates advice and additional information that allows optimal use to be made of
the product.

Warning: This symbol warns users that failure to take the information into account may result in
damage to the equipment. Precautions to be taken and potential consequences are described in the
warning.

Caution! This symbol indicates the safety measures that must be applied by users and technicians to
avoid injury to persons near the equipment. It is essential to follow these measures thoroughly.

Danger, extreme cold! This symbol indicates a hazard related to the ultra-low temperature of the
freezer chamber and its contents.

3. Change log

Updates are listed in the revision history below.

Date

Issue

Changes

Sections

Author

Jan. 2017

0.1

First issue

All

F. LE CAM

Original manual – Iss. 0.1 User manual – BM 3E Freezer 4/ 44

4. Table of contents

1. CERTIFICATE OF CONFORMITY .................................................................................................................. 6

2. WARRANTY .............................................................................................................................................. 7

3. GENERAL INFORMATION .......................................................................................................................... 8

4. SPECIFICATIONS ....................................................................................................................................... 9

4.1 RANGE OF UNITS .................................................................................................................................................. 9

4.2 SPECIFICATIONS ................................................................................................................................................... 9

4.3 POWER SUPPLY ................................................................................................................................................... 9

4.4 REFRIGERATION SYSTEM ........................................................................................................................................ 9

4.5 PERFORMANCE AND AMBIENT TEMPERATURE .......................................................................................................... 10

5. INSTALLATION ........................................................................................................................................ 11

5.1 DELIVERY - UNPACKING ...................................................................................................................................... 11

5.2 ENVIRONMENT CONDITIONS ................................................................................................................................ 11

5.3 ELECTRICAL ENVIRONMENT .................................................................................................................................. 11

5.4 LOCATION ........................................................................................................................................................ 11

5.5 CONSTRUCTION AND INSULATION .......................................................................................................................... 12

5.6 CONTROL CONSOLE ............................................................................................................................................ 12

5.7 LOADING .......................................................................................................................................................... 13

6. GENERAL USE ......................................................................................................................................... 14

6.1 COMMISSIONING ............................................................................................................................................... 14

6.2 MAIN SCREEN ................................................................................................................................................... 15

6.2.1 ESSENTIAL MODEL .............................................................................................................................................. 15

6.2.2 EVOLUTION MODEL ............................................................................................................................................ 15

6.3 HOME SCREEN................................................................................................................................................... 15

6.4 ADJUSTING THE TEMPERATURE SETPOINT ................................................................................................................ 16

6.5 USER PREFERENCES MENU ................................................................................................................................... 18

6.6 MAPPING MENU ................................................................................................................................................ 18

6.6.1 CONFIGURING THE FREEZER ................................................................................................................................. 19

6.6.2 ADDING A SAMPLE ............................................................................................................................................. 20

6.7 SEARCHING FOR A SAMPLE ................................................................................................................................... 21

6.8 PRODUCT INFORMATION ..................................................................................................................................... 22

6.9 STATISTICS ........................................................................................................................................................ 23

6.10 SERVICE / CUSTOMER ADMINISTRATION MENU ........................................................................................................ 24

6.10.1 SETTING THE HIGH AND LOW ALARMS .................................................................................................................... 24

6.10.2 SETTING THE START-UP TIME DELAY ....................................................................................................................... 25

6.11 SETTING THE CO

2

/ N2 INJECTION THRESHOLD ......................................................................................................... 25

6.12 NUMERIC KEYPAD .............................................................................................................................................. 27

6.13 ENTERING A MESSAGE ......................................................................................................................................... 27

6.14 FACTORY MENU ................................................................................................................................................. 27

6.15 DIAGNOSTICS MENU ........................................................................................................................................... 28

6.16 ECO MENU ...................................................................................................................................................... 29

6.17 CLOSING THE FREEZER ......................................................................................................................................... 29

6.18 BATTERY-BACKED ALARM FUNCTIONS .................................................................................................................... 29

6.19 SETTING THE AIR FILTER CHECK INTERVAL ................................................................................................................ 30

6.20 BOSS SYSTEM ................................................................................................................................................... 30

7. OPTIONS ................................................................................................................................................. 31

7.1 REMOTE ALARM ................................................................................................................................................ 31

7.2 CO

2

/ NO2 BACKUP ........................................................................................................................................... 31

7.2.1 RISKS AND PRECAUTIONARY MEASURES .................................................................................................................. 31

7.2.2 COLD BURNS (CRYOGENIC BURNS) ......................................................................................................................... 31

7.2.3 RISK OF ASPHYXIATION ........................................................................................................................................ 31

7.2.4 CO

2

BACKUP ..................................................................................................................................................... 31

7.2.5 N

2

BACKUP ....................................................................................................................................................... 32

Original manual – Iss. 0.1 User manual – BM 3E Freezer 5/ 44

7.3 DELAYING TEMPERATURE RISES ............................................................................................................................. 32

7.4 CHART RECORDER .............................................................................................................................................. 32

7.4.1 TIME SETTING ................................................................................................................................................... 34

7.4.2 REPLACING THE LR6 (AA) BATTERY ....................................................................................................................... 34

7.4.3 REPLACING THE STYLUS IN THE RECORDER ............................................................................................................... 34

8. STORAGE ELEMENTS .............................................................................................................................. 35

9. MAINTENANCE AND CUSTOMER SERVICE .............................................................................................. 36

9.1 USER MAINTENANCE .......................................................................................................................................... 36

9.2 OUTER SURFACES ............................................................................................................................................... 36

9.3 INTERIOR CHAMBER ............................................................................................................................................ 36

9.4 DOOR SEALS ..................................................................................................................................................... 36

9.5 PRESSURE RELIEF VALVE ...................................................................................................................................... 36

9.6 AIR FILTER ........................................................................................................................................................ 36

9.7 STOPPING FOR EXTENDED PERIODS ........................................................................................................................ 36

10. MAINTENANCE PERFORMED BY TECHNICAL SERVICES ........................................................................... 37

10.1 SAFETY RULES.................................................................................................................................................... 37

10.2 SERVICING / SERVICE CONTRACT ........................................................................................................................... 37

11. FAULTS AND ERROR CODES .................................................................................................................... 39

12. SERVICE CONTRACT ................................................................................................................................ 41

13. SAFETY ................................................................................................................................................... 42

13.1 LIQUID CO

2

BACKUP ........................................................................................................................................... 42

13.2 LIQUID NITROGEN BACKUP ................................................................................................................................... 43

14. CUSTOMER SERVICES ............................................................................................................................. 44

Original manual – Iss. 0.1 User manual – BM 3E Freezer 6/ 44

1. Certificate of conformity

Meyzieu, 2 January 2017
Froilabo SAS hereby certifies that the equipment listed below:

BM 3E freezers, versions:

 Essential, -86°C and -45°C, capacity 340 l, 515 l and 690 l,
 Evolution, -86°C and -45°C, capacity 340 l, 515 l and 690 l,

Comply with the applicable standards and technical directives:

 EN 61010-1 – Safety requirements for electrical equipment for measurement, control and laboratory use -

Part 1: general requirements

 2014/35/EU – Low Voltage Directive
 2014/30/EU – EMC Directive, Class A equipment
 97/23/EC – Pressure equipment

Note: These units are not designed for operation in explosive atmospheres (ATEX). Furthermore, they may not be
used to store flammable, corrosive or explosive substances.

Figure 1 BM 3E freezers

Original manual – Iss. 0.1 User manual – BM 3E Freezer 7/ 44

2. Warranty

Froilabo SAS guarantees that the equipment will operate optimally in accordance with the conditions for installation
and use set out in this manual.
The duration of the warranty is 24 months,
extended to:

- 5 years for the fan, compressors, condenser, regulator and PLC.

- 10 years for the VIP insulation.

During this time, in the event that the equipment malfunctions, the warranty is limited to:

- operational enhancement,

- repair or exchange of equipment, without charge,

if it is clear that the fault or failure is related to a material or manufacturing defect.
Any other claims for compensation are excluded.

General information
Froilabo SAS assumes no liability for damage caused by non-compliant use, maintenance operations or unauthorised
modifications.

Compliant use shall mean that the instructions in this manual have been followed and that inspection and
maintenance have been performed.

The photos used in this manual are not contractually binding.

Please read this manual before using the equipment for the first time.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 8/ 44

3. General information

Ensure that all persons using the equipment are suitably trained.
Ensure that all persons installing, using and repairing the equipment are aware of the potential hazards related to
their work and the safety measures to be followed, and that they have read and understood the instructions.
If hazardous or potentially hazardous substances are used, only persons fully familiar with the equipment should
handle these substances. These persons must be capable of making an overall assessment of the potential risks.
Please contact us if you have any questions regarding the use of the equipment or the instructions.
Under no circumstances may Froilabo be held liable for the quality of material stored in the freezers.
Note: The equipment that you have purchased is designed for professional use. Nevertheless, impacts to the frame
and vibrations should be avoided. Ensure that the equipment is inspected at regular intervals appropriate for the
frequency of use. Also check (at least once every two years) that labels relating to safety and unauthorised use are
properly in place. If backup systems using liquid CO2 or nitrogen vapour injection are used, please refer to the
corresponding safety data sheets.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 9/ 44

4. Specifications

4.1 Range of units

There are two ranges of Froilabo freezer, Essential and Evolution, which differ in their man-machine interface.
For each range, several capacities (BM340, BM515 and BM690) and two minimum operating temperatures (-45°C
and -86°C) are available.

4.2 Specifications

FREEZER MODEL

BM340

BM515

BM690

Capabilities

Gross volume (litres)

340

515

690

Number of compartments

2 3 4

Outside dimensions

Height (mm)

1280

1640

2000

Width (mm)

875

875

875

Depth (mm)

970

970

970

Clearance required at the rear of the unit (mm)

200

200

200

Maximum dimension with door open (mm)

1750

1750

1750

Maximum width with door open

1150

1150

1150

Maximum door opening angle

110°

110°

110°

Gross internal dimensions excluding racks

Height (mm)

716

1076

1436

Width (mm)

630

630

630

Depth (mm)

752

752

752

Weight (kg)

223

267

330

Power (Watts)

1100

1500

1500

Absorbed current, 230 V 50 Hz model (A)

6 8 8

Absorbed current, 120 V 60 Hz model (A)

12

16

16

4.3 Power supply

See the manufacturer’s plate at the rear of the unit. Two models are available:

- Voltage 230 V AC ±10%, 50 Hz, protected by aM 12 A fuse.

- Voltage 120 V AC ±10%, 60 Hz, protected by aM 20 A fuse.

4.4 Refrigeration system

FREEZER MODEL

BM340

BM515

BM690

Two-stage -86°C models

Stage 1 hermetic compressor power (Watt)

581

778

778

Stage 2 hermetic compressor power (Watt)

378

581

581

Stage 1 R417a refrigerant – load (g)

580

600

600

Stage 2 R508b refrigerant – load (g)

300

300

300

Single-stage -45°C models

Hermetic compressor power

378

581

778

Isceon 89 refrigerant – load (g)

600

600

600

Capillary expansion

yes

yes

yes

Air condenser as standard

yes

yes

yes

Original manual – Iss. 0.1 User manual – BM 3E Freezer 10/ 44

4.5 Performance and ambient temperature

The BM freezer is factory set to optimise its electricity consumption.
On the -86°C model, the setpoint can be adjusted from -55°C to -87°C. The default setting is -80°C.
On the -45°C model, the setpoint can be adjusted from -20°C to -45°C. The default setting is -45°C.

The ambient temperature (between +18°C and +35°C, ideally between 23°C and 25°C) also has a significant impact on
the electricity consumption of the equipment.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 11/ 44

5. Installation

5.1 Delivery - Unpacking

Use a pallet truck to move the freezer on its pallet. The freezer must be supported during unpacking to avoid any risk
of toppling. The unit may be placed on the floor and manoeuvred on casters. Once the unit has been placed in the
desired location, remove the protective plastic film and chocks.
Froilabo freezers are delivered on a pallet with an unloading ramp. This means that no special equipment is required
to unload them from the pallet. Please refer to the handling and unpacking sheet affixed to the unit.
Do not use sharp objects as they might damage the paintwork.
Note: After unpacking, inspect (if possible with the carrier present) the condition of the unit and its accessories. If any
transport damage is observed, state the details on the delivery note and inform Froilabo without delay.

After receipt, please check the items delivered:

BM 3E – 340 litres

BM 3E – 515 litres

BM 3E – 690 litres

1 non-detachable electrical power lead

1 filter cassette

1 set of two keys

1 pressure relief valve + filters

1 manual

1 shelf

2 shelves

3 shelves

2 brackets

4 brackets

6 brackets

5.2 Environment conditions

The freezer is designed for use under the following ambient conditions (in accordance with EN 61010-1):

 Indoor use
 Maximum altitude: 2000 m
 Ambient temperature range between 5°C and 40°C
 Maximum relative humidity of 80% for temperatures up to 22°C
 Supply voltage ripple < ±10 % of rated voltage.
 Supply network voltage surges: category II (Standard IEC 60364-4-44)
 Maximum level of room contamination: 2

BM 3E freezers are Class A devices within the meaning of the EMC Directive.

5.3 Electrical environment

A 230 V / 50 Hz or 120 V / 60 Hz single-phase electrical power supply is required, as specified on the manufacturer’s
plate at the rear of the unit.

The freezer must be powered via a safety mechanism, such as a correctly rated differential circuit breaker, which
automatically cuts off the power supply if an insulation fault occurs.
To enable the unit to be electrically cut off, the circuit breaker must be readily identifiable and within reach of the
operator.

5.4 Location

To minimise power consumption and achieve the specified performance, place the freezer in a ventilated location,
away from sources of heat (radiators, heaters, etc.) and avoid locations exposed to sunlight. Place it on a flat surface.
For correct operation, it is essential that the freezer be placed level. Use rigid chocks if necessary.
Ensure that there are no obstacles (walls, equipment) that impede the ventilation of the freezer (air inlet and outlet).
Lower the two front legs, turning by an additional quarter-turn after they touch the ground.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 12/ 44

Room temperature should not exceed 35°C. The moisture content in the air must not exceed 50% RH. The use of an
air conditioning system can considerably extend the service life of the compressors.
If backup systems using liquid CO2 or nitrogen vapour injection are used, please refer to the corresponding safety data
sheets in section 13 SAFETY.

5.5 Construction and insulation

The self-contained outside casing is made from zinc electroplated steel sheet protected by epoxy paint.
The inner chamber is made from stainless steel sheet. Thermal insulation is provided by vacuum and polyurethane
foam insulating panels. The insulated swivel door is mounted on a hinge.
The door is closed and sealed by a progressive sealing handle.

5.6 Control console

The control console is located towards the top of the door. It comprises a main screen and a touch-sensitive alarm
window. The main screen is touch-sensitive only on the Evolution model.

Interactive touch-sensitive screen (Evolution
model)

Touch-sensitive alarm window

Lockable handle
Filter cassette

Lower door

Front

Opening of lower door panel

Stabilising legs

Alarm warning lights

Disk recorder unit (optional)

Screen (Essential model)

Essential model

Evolution model

Main screen

Touch-sensitive

alarm window

Touch-sensitive
main screen

Touch-sensitive

alarm window

Original manual – Iss. 0.1 User manual – BM 3E Freezer 13/ 44

5.7 Loading

In order to avoid any damage to the freezer and ensure that the specified technical performance is attained, the
following instructions must be followed:

- Do not place highly corrosive substances in the freezer.

- Do not place explosive or highly flammable substances in the freezer.

- Leave at least 3 cm clearance along internal walls.

- Do not pull out several shelves at once.

In order to avoid any risk of a heavily loaded freezer toppling, do not pull out several sliding shelves and/or drawers
simultaneously.
Do not under any circumstances exceed the maximum allowable load for each sliding shelf or drawer.

These freezers are not explosion-proof.

The interior surfaces of the freezer and the contents can be extremely cold (-86°C):
Protect yourself accordingly (in particular by wearing suitable gloves).

It is possible to replace a drawer with a shelf. A shelf cannot, however, be replaced with a drawer.

Freezer model
Storage level

BM340

BM515

BM690
Level 4

/ / 1 shelf

Level 3

/

1 shelf

1 shelf or 1 drawer

Level 2

1 shelf

1 shelf or 1 drawer

1 shelf or 1 drawer

Level 1

No fixtures

No fixtures

No fixtures

Original manual – Iss. 0.1 User manual – BM 3E Freezer 14/ 44

6. GENERAL USE

6.1 Commissioning

Follow the instructions in the correct order:

1) Install the drawers or shelves in each level.

2) Connect the freezer to a power outlet (230 V 50 Hz 16 A + neutral + earth) protected by a 30 mA differential circuit

breaker (the compressor(s) and fan will start up).

Press the On/Off button.

3) The screen displays “Froilabo” (start-up phase), then shows the temperature inside the chamber.

4) Adjust the setpoint (-45°C or -80°C by default, depending on model).

See the procedure in section 6.4 Adjusting the temperature setpoint.

5) Wait until the freezer reaches the set temperature (3 to 6 hours depending on model).

6) Load the freezer.

Note 1: The temperature in the freezer may rise if it is loaded with “hot” products. The high temperature alarm may

be triggered.

Note 2: The switch cuts off only the internal 24 V power supply required in particular for the processor and display.
The freezer is still powered as long as the power cable is connected to the mains.
Any electrical maintenance must be performed by qualified staff.

Rear view

On/Off switch

Switch position (set back within the

freezer)

Close-up view of the switch

Original manual – Iss. 0.1 User manual – BM 3E Freezer 15/ 44

6.2 Main screen

6.2.1 Essential model

Image

Description

The image opposite is displayed at all times.

In the event of a malfunction, the screen turns off and the
red indicator flashes. A buzzer sounds intermittently.

6.2.2 Evolution model

Image

Description

Command

When the freezer is in use, the screen opposite is
displayed at all times.

Main display

Temperature measured

The envelope flashes when a new message is added.

The biohazard or radiation symbols may be shown at the
top of this screen. These visual reminders can be
configured in the user preferences.

6.3 Home screen

Press anywhere on the main display to show the Home screen.
The Home screen provides access to all available menus.
If the screen is not touched for 1 minute, the main display automatically resumes.

Image

Description

Command

Home screen

User preferences menu

Mapping menu

Information menu

Statistics menu

Servicing menu

Factory menu (password required)

Original manual – Iss. 0.1 User manual – BM 3E Freezer 16/ 44

Diagnostics menu

ECO menu

Access to a free text zone in order to leave messages for
subsequent users.
The logo flashes to indicate the presence of a new
message.

Adjust the temperature setpoint

Return to the main display

6.4 Adjusting the temperature setpoint

Image

Description

Command

On the Evolution model, the temperature setpoint adjustment is accessible from the Home screen.

Home screen

To adjust the temperature setpoint, press the
pictogram opposite on the Home screen.

Numeric data entry

The numeric data entry screen is used to enter a
negative value within the authorised range.

Enter the desired setpoint.

Confirm.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 17/ 44

On the Essential model, the setpoint can be adjusted on the display at the bottom of the unit.

Open the door at the bottom right to access the
display.

Essential display

Adjust the value using the two buttons.
No confirmation is necessary; the value is stored
directly.

Actual
temperature
(in red)

Temperature

setpoint

(in green)

Original manual – Iss. 0.1 User manual – BM 3E Freezer 18/ 44

6.5 User preferences menu

The user can configure certain settings to customise use of the freezer.

Image

Description

Command

From the Home screen, access the user preferences.

User preferences menu

Screen brightness setting

Standby timer

Date setting

Time setting

Choice of temperature unit: °C / °F
Default value: °C

Display of estimated sample temperature.
Default value: ON
When set to OFF, the chamber air temperature is
displayed.

Display of biological risk warning: ON/OFF. Default value:
OFF.
When set to ON, the logo is displayed at the top of the
screen.

Display of radiation risk warning: ON/OFF.
Default value: OFF.

Return to previous menu

6.6 Mapping menu

General menu giving access to the three mapping-related functions.
This menu is an option on Evolution models.

Image

Description

Command

From the Home screen, access the mapping menu.

Mapping menu

Search for a sample

Enter a new sample

Configure the freezer storage space

Return to the previous menu without saving changes

Pressing one of these buttons brings up a username entry
screen.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 19/ 44

6.6.1 Configuring the freezer

The freezer must be configured to specify the type of storage elements incorporated. Configuration thus entails
defining the types and locations of drawers and shelves.

Image

Description

Command

Mapping menu

From the Mapping screen, access the freezer
configuration menu.

Enter the access code (five digits).

Confirm.

Select the freezer capacity by pressing the
corresponding value. The elementary grid
corresponding to the capacity is displayed (factory-set
default value).

Note: Several sample numbers can be stored in the
same location.

Select a shelf two units wide.

Select a drawer one unit wide.

Select another accessory two units wide.

Select another accessory one unit wide.

Empty space.

Exit the menu, confirming the configuration.

Erase the last item entered.

The elementary location being configured is
highlighted.

Return to the previous menu without saving changes.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 20/ 44

Example after configuring the top three

shelves.

Drawer elements are shown in blue.

Shelf elements are shown in cream.

The cursor is positioned on the next zone to be edited.

6.6.2 Adding a sample

Image

Description

Command

From the Mapping screen, access the Add sample menu.

Enter the access code (five digits).

Confirm.

Press the blue zone to enter a sample identifier. A data
entry keyboard is displayed.

Enter the sample identifier.

Save and confirm the data entered.

Select where the sample will be stored.
Drawer (blue)

or

Shelf (pink)

Original manual – Iss. 0.1 User manual – BM 3E Freezer 21/ 44

Select the drawer or shelf by pressing the corresponding
number.

The selected drawer number is displayed in the location.

Confirm the data entered.

6.7 Searching for a sample

Image

Description

Command

Searching for a sample

Press the blue zone to enter a sample identifier. A
data entry keyboard is displayed.

Enter the sample identifier.
Confirm the data entered.

The search is performed.

The corresponding storage area is highlighted.
The shelf number is shown in the centre.

The sample record can be deleted.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 22/ 44

6.8 Product information

Image

Description

Command

From the Home screen, access the Product
information menu.

The information cannot be modified. The following
information is displayed, in order:

• Model name

• Capacity in litres

• Serial number

Maximum electrical power rating

• Electricity consumption converted to BTU/h
(1 W = 3.412141633 BTU/h)

• System software version

• Screen software version

L: Stage 1 refrigerant type
Stage 1 load
H: Stage 2 refrigerant type
Stage 2 load

Return to previous menu

Original manual – Iss. 0.1 User manual – BM 3E Freezer 23/ 44

6.9 Statistics

Data is stored in the unit. It can be displayed either for the last 24 hours or for the entire operating life of the freezer.

Image

Description

Command

From the Home screen, access the Statistics menu.

(*)
CP1: Stage 1 (bottom) compressor
CP2: Stage 2 (top) compressor

Number of start-ups (e.g. 10) and number of start-ups
per hour (e.g. 3/h) for each compressor.(*)

Compressor usage rate.
This displays the compressor operating time relative to
the total operating time for the unit.

Compressor usage time, in hours.

Number of times the door has been opened, and
average door open time.

Electrical power consumption of the unit.

Selection of statistical data displayed:

“24H”: last 24 hours
"4EVER”: since the freezer was commissioned

Access to the temperature graph for the last 10 hours.
See below.

Access to the fault history.
Return to previous menu

Temperature graph

From the Statistics menu, access the temperature
graph.

The graph shows the actual temperature, the high and
low alarm levels and the temperature setpoint. If
possible, link the axis limits to the temperature alarm
levels. Allow for return.

From the Statistics menu, access the fault history.

Fault history

List of the last 10 errors, followed by the dates and
times when the fault occurred and ceased.

Access the error codes

Original manual – Iss. 0.1 User manual – BM 3E Freezer 24/ 44

6.10 Service / customer administration menu

Image

Description

Command

From the Home screen, access the Servicing menu.

Enter the access code (five digits).
Default code: “11111”.

Confirm.

Servicing menu

Top sensor offset
(protected by servicing password)

Bottom sensor offset
(protected by servicing password)

Door open alarm delay

Low temperature alarm threshold

High temperature alarm threshold

Emergency injection configuration: OFF / CO2 / N2

Emergency injection trigger threshold
Start-up delay time

Return to the main display

6.10.1 Setting the high and low alarms

Image

Description

Command

Servicing menu

Low temperature alarm threshold
Set the desired temperature at which the alarm will be
triggered.

High temperature alarm threshold
Set the desired temperature at which the alarm will be
triggered.

Return to the main display

Original manual – Iss. 0.1 User manual – BM 3E Freezer 25/ 44

6.10.2 Setting the start-up time delay

If several freezers are installed on the same electrical network, the start-up time delay can be used to start the units in
turn in order to avoid momentarily overloading.

Image

Description

Command

Servicing menu

Start-up delay time
Set the delay before the unit starts up.

After the freezer is powered up, the first stage
compressor will start only after this delay has passed.

Note:
This parameter can also be set on the Essential model. This operation must be performed by a qualified technician. It
entails opening the rack and setting switches on the Artic board.
The switches marked “SW1” should be set to allow at least 5 seconds between each start-up. The number set in
binary corresponds to a delay of 30 seconds + the number of seconds encoded in binary.

Image

Description

Command

Artic board

Set the desired value based on the binary codes
shown below.

Switch No.

1 2 3 4 5 6 7 8 Binary total

Time delay
(in seconds)

Binary weight

8 7 6 5 4 3 2 1 Example 1

0 0 0 0 0 0 0 0 0

30 (30+0)

Example 2

0 0 0 0 0 1 0 1 5

35 (30+5)

Example 3

0 0 0 0 1 0 1 0 10

40 (30+10)

Example 4

0 0 0 0 1 1 1 1 15

45 (30+15)

Example 5

0 0 0 1 0 1 0 0 20

50 (30+20)

Example 6

0 0 0 1 1 0 0 1 25

55 (30+25)

etc. … …

.

6.11 Setting the CO

2

/ N2 injection threshold

To use emergency injection, CO2 / N2 injection must be enabled and the injection threshold must be set.

Warning: When the freezer is installed, injection must be enabled only once the temperature setpoint has been

reached, otherwise the gas cylinders will be discharged before the temperature drops for the first time.

Warning: Using these gases is hazardous. Please refer to the safety data sheet in section 13 SAFETY.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 26/ 44

Image

Description

Command

Servicing menu

Emergency injection configuration: OFF / CO2 / N2
Select the corresponding mode.

Emergency injection trigger threshold
Set the desired temperature.

Return to the main display

Original manual – Iss. 0.1 User manual – BM 3E Freezer 27/ 44

6.12 Numeric keypad

When a numeric parameter is to be entered, the keypad below is displayed.

Image

Description

Command

Numeric data entry

Save the change and return to the previous screen.

Delete the last digit entered.

Return to the previous menu without saving changes.

6.13 Entering a message

A text message, visible to all users, can be entered.

Image

Description

Command

Press the text field.

Delete the entire message

Return to the main display

Access lower case characters

Access upper case characters

Access digits and symbols

6.14 Factory menu

Image

Description

Command

From the Home screen, access the Factory menu.
This menu is reserved for use on the production line.
Access is protected by a factory password.

Return to previous menu

123456A

-83

.2

°C

123456A

-83

.2

°C

free text field

Original manual – Iss. 0.1 User manual – BM 3E Freezer 28/ 44

6.15 Diagnostics menu

Image

Description

Command

From the Home screen, access the Diagnostics menu.
Temperature of the top sensor.

Optional.

Temperature of the bottom sensor (analogue gauge).

Ambient temperature.
Optional.

Instantaneous power consumption (analogue gauge), in
Watts.

Door status sensor:
Green tick: door closed
Red cross: door open

Top compressor (H or CP2) control:
State of power contactor: ON/OFF

Bottom compressor (L or CP1) control:
State of power contactor: ON/OFF

Condenser fan control:
State of power contactor: ON/OFF

Battery low signal:
Green tick: battery charged
Red cross: battery discharged

Heating gasket control: ON/OFF
Default value: ON.

Top compressor (H) overpressure sensor:
Pressure value in bar.

Bottom compressor (L) overpressure sensor:
Pressure value in bar.

Temperature at intermediate condenser

Condenser fan flow measurement: percentage of
maximum flow measured in factory.

User presence detection:
Green tick: Movement is detected in front of the
keyboard.
Red cross: User not present or motionless in front of the
door.

Remote alarm relay test

CO2 / N2 solenoid valve test
Return to previous menu

H

L

H

L

Original manual – Iss. 0.1 User manual – BM 3E Freezer 29/ 44

6.16 ECO menu

The ECO menu can be used to define the freezer’s electricity consumption settings for each day of the week.

Image

Description

Command

From the Home screen, access the Factory menu. This
menu is reserved for use on the production line.
Access is protected by a factory password.

Select the day of the week to be configured based on the
settings shown on the screen.
Monday = 1 to Sunday = 7

ECO mode: enabled (ON) or disabled (OFF).

Set the start time for ECO mode.
A numeric keypad is displayed. Enter the start time and
then confirm.

Set the stop time for ECO mode. A numeric keypad is
displayed. Enter the end time and then confirm.

Door seal heating mode: enabled (ON) or disabled (OFF).
In the OFF position, frost may build up on the seal, which
may then harden.

User presence detection mode: enabled (ON) or disabled
(OFF).
If this mode is disabled, the screen does not turn on when
movement is detected in front of the door.

Energy saving advice is displayed.

Setting the ECO mode setpoint.
A numeric keypad is displayed. Enter the desired
temperature and then confirm.

indication of the electricity consumption corresponding to
the settings.
Green: economical setting.
Red: setting with higher electrical consumption.

Return to previous menu

6.17 Closing the freezer

The freezer has a progressive sealing handle with a double-acting mechanism to facilitate opening and closing of the
door. The freezer door can be locked using a key-operated lock on the side of the handle.

6.18 Battery-backed alarm functions

In the event of a mains power cut, the buzzer, remote alarm and CO2 injection (optional) functions remain active,

powered by a backup battery supplied as standard. The temperature display is also maintained.
Warning: The compressors are no longer powered and the freezer thus does not cool.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 30/ 44

6.19 Setting the air filter check interval

This operation must be performed by a qualified technician. It entails opening the rack and setting switches on the
Artic board.

Image

Description

Command

Artic board

Set the desired value based on the codes shown
below.

The SW2 switches must be set exactly as shown in the table below:

Switch No.

1 2 3 4 Time delay

Case 1

0 or 1

0 0 0

6 months

Case 2

0 1 0 0 8 months

Case 3

0 0 1 0 12 months

Case 4

0 0 0 1 14 months

6.20 BoSS system

The BoSS system ensures that the freezer will operate in the event of a malfunction on the 24 V circuit board provided
that the mains supply is present. The compressors are then powered continuously, but are not regulated by the PLC.

Your samples are thus safe, with no risk of a temperature rise.

Trigger principle:

1. If the 24 V supply to the regulator is cut off due to a fault on the circuit board, the regulator is powered by the 24 V
batteries, the alarm light is not lit and the freezer operates normally, powered by the backup batteries.

2. If the battery voltage drops to 20 Volts (±2 V) or if the controller does not activate its heartbeat signal, the BoSS
system is triggered, the alarm indicator and buzzer on the freezer front panel are triggered and the regulator display is
lit. The compressors are connected directly to the 230 V supply with no regulation system.
The temperature in the freezer may fall to –90°C ±2°C.

3. After 40 hours (±1 hour), the regulator display stops. The compressors remain in continuous operation with no
regulation, and the alarm indicator is not lit.

The freezer can operate under the BoSS system for several days without difficulty. However, Froilabo’s service

department should be contacted to schedule a repair.

Warning: THESE SPECIFICATIONS WERE OBTAINED USING NEW BATTERIES CHARGED FOR AT LEAST 24 HOURS.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 31/ 44

7. OPTIONS

7.1 Remote alarm

The connector located at the rear of the unit can be used to connect a remote alarm.
We highly recommend using the NC contact, which also enables an alarm to be transmitted if one of the wires is
disconnected.
Maximum allowable current: 5 A at 30 V DC, 5 A at 250 V AC

Note: After connecting the wires, screw the connector to its base to avoid any risk of the wires being pulled loose.

7.2 CO

2

/ NO2 backup

7.2.1 Risks and precautionary measures

Whenever a CO2 or N2 backup system is used, it is essential to refer to the safety data sheet (see sections 13.1 and

13.2).

7.2.2 Cold burns (cryogenic burns)

The “extreme cold” pictogram must be used to warn of this hazard.
If CO2 or N2 injection has been used, the door must not be opened, in order to avoid any risk of cryogenic burns.

In the event of an accident: WARNING! The temperature of the injury site must be returned to body temperature as

quickly as possible. The appearance of a cryogenic burn initially seems to be minor and does not cause concern. Rinse
the burn with warm water for at least 15 minutes.
Cover with a sterile dressing and consult a doctor.

7.2.3 Risk of asphyxiation

The “asphyxiation risk” pictogram must be used to warn of this hazard.
Cold vapours are heavier than air and can accumulate in confined locations, particularly at or below floor level.
These gases can result in asphyxiation, even from the second breath, leading to unconsciousness (oxygen content of
less than 18%).
To avoid this hazard:

- Ensure that storage and use areas are well ventilated.

- Do not discharge nitrogen into locations where it could build up to dangerous levels (sewers, basements, pits, closed
rooms).

In the event of an accident: If asphyxiation occurs, the rescuer must use a self-contained breathing apparatus to

access the victim, or must be able to quickly ventilate the room to a sufficient extent without entering it.

7.2.4 CO

2

backup

General remarks

CO2 The pressure in the cylinders and hoses reaches 70 bar. It is therefore recommended to call on qualified

personnel for any servicing of this system.
Cylinders of liquid CO2 must be used.
Connection
Principle: The freezer injects liquid CO2 into the chamber if the interior temperature rises. The temperature is
regulated by the CO2 injection solenoid valve.

FREEZER AT -80°C, ALARM THRESHOLD SET TO -60°C

Contact position above

alarm threshold

Contact position below

alarm threshold

Temperature above alarm

threshold (e.g. 59°C):

the contact switches to NC

Temperature below alarm

threshold (e.g. 80°C):

the contact is at NO

Original manual – Iss. 0.1 User manual – BM 3E Freezer 32/ 44

Connect the hose to the solenoid valve located at the rear of the freezer, then to the distribution manifold or the CO2
cylinder, after checking that Teflon tape is applied to the thread.
Warning: If the ambient temperature is higher than +37°C, all of the CO2 in the cylinder will be transformed to a gas.
The pressure may exceed 90 bar (temperature of +40°C). If gaseous CO2 is injected into the freezer chamber, no
cooling effect occurs.
CO2 detector
When CO2 or N2 are injected, the oxygen content in the air gradually decreases. To avoid potential asphyxiation of
personnel in the room, Froilabo can supply CO2 detectors. An audible alarm is sounded if the oxygen level is low.
To order or install a CO2 detector on an existing system, please contact our customer service department.

7.2.5 N

2

backup

Principle: The freezer injects liquid nitrogen (vapour phase) into the chamber if the interior temperature rises. The
solenoid valve regulates the temperature by pulsing the N2 injection.

CO2 solenoid valve
Connecting the N2 option

1. Connect the N2 hose supplied with the freezer (1).

2. Check that all couplings are properly tightened.

3. Connect the liquid nitrogen supply.
Parts to be assembled:

- ¼” copper pipe + short nut + safety valve (part A)

- pre-assembled kit (tee piece + two male-female unions) (part B).

1. Check that the pre-assembled kit is covered with Teflon tape.

2. Screw the pre-assembled kit onto the solenoid valve, ensuring that the tee points upwards.

3. Connect the copper pipe to the tee.

4. Attach the copper pipe to the freezer using Colson clamps, ensuring that the safety valve points downwards.

5. Check that all couplings are properly tightened.

6. Connect the liquid nitrogen supply.

7.3 Delaying temperature rises

Principle: The cryo-accumulator system is incorporated into the useful volume of the freezer and does not reduce its
storage capacity.
It provides additional autonomy before the temperature rises due to refrigeration stoppages.
For example, the time taken for the temperature in a BM 3E 690L freezer to rise from -80°C to -40°C is:

- 6½ hours without a cryo-accumulator.

- 13 hours with a cryo-accumulator.
Note: These tests were conducted with a freezer half full, at an ambient temperature of 25°C, without opening the
door.

7.4 Chart recorder

Froilabo proposes an optional chart recorder, capable of recording the temperature over a 24-hour or 7-day period
(selectable on the unit). Two models are available: disc or digital.
Description

Changing the paper disc (diagram)

1. Unlock and open the recorder door.

Lock

Clips

Chart

Stylus arm

Stylus

Knurled nut

Original manual – Iss. 0.1 User manual – BM 3E Freezer 33/ 44

2. Raise the stylus arm.

3. Unscrew the knurled nut.

4. Remove the chart.

5. Position the new disc, inserting it beneath the clips, and then tighten the knurled nut without forcing.
Note: The chart is perfectly aligned with the axis.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 34/ 44

7.4.1 Time setting

Manually turn the axis clockwise to the desired date and time.
Use the end of the stylus as a reference point. It is essential to turn clockwise to avoid backlash in the timer
mechanism.

7.4.2 Replacing the LR6 (AA) battery

1. The battery compartment is located underneath the paper disc in the recorder (1).

2. Remove the paper disc (1).

3. Remove the drive mechanism and turn it upside down (2 and 3).

4. Replace the battery, ensuring that the polarity is correct (4).

5. Replace the drive mechanism (5).

6. Position the disc beneath the clips and then tighten the knurled nut (knurling on outside) without forcing (6).

7. Set the time (7).
Note: The operating speed can be selected (1× 24 h or 7× 24 h). To change the speed, move the drive mechanism
switch downwards (8).

7.4.3 Replacing the stylus in the recorder

The felt stylus is supplied in a sealed silver-coloured bag.

1. Raise the arm.

2. Remove the stylus, holding the arm between your thumb and index finger (photo).

3. Insert the new stylus.

4. Remove the protective cap.

5. Lower the arm.

6. Set the time.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 35/ 44

8. STORAGE ELEMENTS

To facilitate storage in your freezer, an extensive range of storage elements is available: fixed shelves, sliding shelves,
drawers, etc.
For further information, please contact our sales department.

Drawers (ELMP690)

Shelves (EL.E)

Drawer unit (EL.T)

Original manual – Iss. 0.1 User manual – BM 3E Freezer 36/ 44

9. MAINTENANCE AND CUSTOMER SERVICE

9.1 User maintenance

MICROBIAL BUILD-UP
Due to the intense cold, microorganisms from packaging, handling and contact can survive and remain fully virulent.
All necessary precautions must be taken when accessing freezers:

- Outer surfaces must be cleaned regularly.

- Gloves must be worn.

- The door must be opened only briefly.

- Tissue samples and packaging must be handled under a laminar flow hood.
The freezer must be switched off before cleaning. A water jet must not be used for cleaning, to avoid splashing the
freezer.

9.2 Outer surfaces

Wash with warm water and soap or a non-corrosive neutral detergent. Rinse and dry thoroughly.

9.3 Interior chamber

Bleach must not be used, even at very low concentrations.
The stainless steel surfaces must not be cleaned with steel wool or any other abrasives.

9.4 Door seals

The door seal is heated by a resistive wire located within the joint in order to minimise frost build-up. Frost may
nonetheless build up between the lips of the seal, especially if the door is opened frequently. The silicone seal should
be cleaned with dry compressed air or using the plastic blade supplied by Froilabo.

9.5 Pressure relief valve

Froilabo freezers have a pressure relief valve enabling the chamber to be quickly returned to ambient pressure, either
when opening the door or after injection of CO2 or N2.
Frequent opening of the doors increases humidity in the freezer and may result in ice forming in the air intake tube in
which the pressure relief valve is located. A set of calibrated foam blocks is placed in the air intake tube to minimise
this phenomenon.
If the air intake tube is blocked by ice, opening the door becomes difficult.
If this occurs, remove the safety valve and the calibrated foam blocks and then insert a screwdriver (or other long,
thin object) in the air intake tube and push the ice into the freezer chamber.
Clean the pressure relief valve (on the side of the unit) and the calibrated foam blocks on a regular basis (every one to
three months, as needed). Ensure that the valve and blocks are replaced correctly.

9.6 Air filter

In order to maintain the refrigeration performance of the freezer and prolong the life of the compressors, an air filter
is located on the front panel, in front of the condenser.
This filter must be cleaned of dust as often as necessary. To do this, simply remove the filter and wash it (using water
with no cleaning products). After drying (without wringing) the filter, put it back in place.
Freezers must not be operated without filters (except in a clean room).

9.7 Stopping for extended periods

If the unit is to remain unused for a long period of time, the following operations should be performed:

1. Turn off the freezer.

2. Unplug the mains lead.

3. Defrost, clean and wipe the chamber.

4. Leave the door ajar to avoid any unpleasant odours.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 37/ 44

10. MAINTENANCE PERFORMED BY TECHNICAL SERVICES

10.1 Safety rules

Before performing any servicing on the freezer, turn it off using the On/Off button and disconnect it from the mains.
Cut off at the main fuse holder (on the electrical cabinet, accessible by dismantling the bottom right section.

Image

Description

Remove the rack mounting system.

Withdraw the rack and place it carefully on the floor.

10.2 Servicing / service contract

In the same way that motorists service their vehicles to keep them in the best possible working order, so the use of a
freezer requires a certain minimum level of maintenance in order to keep it in good working order.
Accordingly, an annual service is recommended, to check various aspects (performance, safety, alarms), to pre-empt
certain failures and to carry out preventive actions to minimise the risks of unexpected shutdowns. This service may
be performed by the customer’s maintenance department, if it possesses the equipment needed and has received
training from Froilabo, or by a Froilabo technician during an annual inspection. Notwithstanding the above, any
serious issues will require a visit from our maintenance service, or diagnosis and assistance by telephone.
Inspection points:

- Fully clean the condenser and replace the filter.

- Check the refrigerant load.

- Check the motor current loads.

- Check the safety mechanisms, alarms and thermostats.

- Check all mechanical parts (latches, chamber fixtures)

- Check the internal chamber temperature.

- Check the refrigerating circuit for leaks.
Check the compressor dampers (vibration reduction).

- Replace the stylus in the recorder.

- Check the fan bearings.

Depending on the type of service contract, Froilabo undertakes to respond within set lead times in the event of a
failure.
To take out a service contract, please return the service contract request form (see section 12 SERVICE CONTRACT) by
fax.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 38/ 44

Image

Description

Command

Power and control rack

Remote alarm connector

Power contactors (×3)
The BoSS protection system makes use of these

normally-closed power contactors.

Fuse holder

24 V supply

Artic board

Buzzer

Regulator (Essential model)

Original manual – Iss. 0.1 User manual – BM 3E Freezer 39/ 44

11. FAULTS AND ERROR CODES

On the Evolution model, faults are indicated on the screen on the door.

Image

Description

Command

Evolution model:
If a fault occurs, the screen turns red.
The temperature is displayed.

Press the “i” button for details of the fault.

A pictogram illustrating the fault is displayed in the
centre of the screen.

A brief description and the action to be taken are
displayed in English beneath the pictogram.

On the Essential model, faults are indicated on the display at the base of the unit. The same pictograms are used on
the Essential and Evolution models.

Image

Description

Error
codes

Pictogram

Possible
states

Indicator LEDs can be seen by
opening the door at the bottom
right.

/ /

Intermediate exchanger information
(no fault even if lit)

/

First LED, no logo

Temperature alarm:

1 & 2 / /

Temperature too high

1 Steady

Temperature too low

2 Flashing

CO2 or N2 injection required

3 Steady

Door open

4 Steady

Backup battery discharged

5 Steady

Power supply cut
& BoSS system activated

6

+

Steady

Not used 7 /

Steady

Compressor alarm:

8 & 9 / /

Compressor 1 pressure too high

8 Steady

Compressor 2 pressure too high

9 Flashing

Air filter dirty or missing.

10 Steady

Main temperature sensor fault

11 Steady

Original manual – Iss. 0.1 User manual – BM 3E Freezer 40/ 44

Not used

12

N/A

/

Not used

13

N/A

/

Intermediate exchanger temperature
sensor fault

14 Flashing

Compressor 1 contactor fault

15 2 pulses

Compressor 2 contactor fault

16 2 pulses

Fan contactor fault

17 Flashing

Compressor 1 pressure sensor fault

18 3 pulses

Compressor 2 pressure sensor fault

19 3 pulses

Target temperature not reached

20

+

Steady

Not used

/ /

Not used

/ /

Original manual – Iss. 0.1 User manual – BM 3E Freezer 41/ 44

12. SERVICE CONTRACT

Original manual – Iss. 0.1 User manual – BM 3E Freezer 42/ 44

13. SAFETY

13.1 Liquid CO

2

backup

PRECAUTIONS FOR THE USE OF CO

2

Properties of CO

2

Does not sustain life or combustion.
Present in low concentrations (0.03%) in air.
Makes the atmosphere unbreathable at concentrations exceeding 3%.
Accelerates respiratory rate, induces faintness, vomiting, coma or even death.
Heavier than air (d=1.53).
Non-flammable, colourless gas with a slightly acidic odour at high concentrations.
When liquid CO2 is released at atmospheric pressure, carbon dioxide snow is generated at a temperature of –80°C.
Ensuing risks:
Risk of asphyxiation. Loss of consciousness occurs when concentration exceeds 8 to 10%.
CO2 builds up in low areas.
Risk of frostbite.
Risk of corrosion of steels in the presence of humidity.
Essential precautions to be taken
Areas where CO2 is stored or used must be well ventilated (extraction or ventilation at both high and low levels in the
room).
Areas liable to contain an unbreathable atmosphere must be indicated with a CO2 – risk of asphyxiation hazard
pictogram.
Never enter a room that has contained CO2 without taking predefined precautionary measures.
Eliminate links between areas where CO2 is stored or used and low points (pits, drainage channels, basements) where
it could accumulate and render the atmosphere unbreathable.
Use a CO2 concentration detector (or O2 concentration detector) to check that the CO2 concentration is less than 0.5%
(as recommended by INRS, the French National Research and Safety Institute).
In the event of an incident or accident
If asphyxiation has occurred
Check the oxygen concentration in the room and then take the victim to the open air (taking an insufflator with you).
Begin artificial respiration and call the emergency services.
In the event of a leak
Do not enter the room without breathing apparatus if the carbon dioxide concentration is higher than 3%.
Close the valve on the cylinder.
Air the room at length, ensuring that low points are ventilated.
PRECAUTIONS WITH REGARD TO EQUIPMENT
Warning: cylinders are pressurised!
Handle cylinders with care.
Secure cylinders in place.
Do not expose cylinders to excessive temperatures.
Treat valves with care (operate gently, do not dismantle or lubricate valves).
Ensure that hoses and pressure regulators are fit for use. Check the condition of gaskets and use original parts only.
Before installing the pressure regulating valve, open the valve briefly to expel dust (do not stand in front of the valve
outlet at this time).
Never transfer gas from one cylinder to another.
Never lay a cylinder on its side during use.
After use:
Close the valve carefully.
Drain the outlet circuit.
Loosen the adjustment screw on the pressure regulating valve.
Close the valve on the receiving equipment.
Regulations apply to the transportation of cylinders.
Frames must be handled with care in the same way as cylinders.
Users of the equipment, who are fully aware of the conditions of use and thus best placed to monitor the equipment,
are solely responsible for proper use.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 43/ 44

13.2 Liquid nitrogen backup

Strict rules must be followed when handling cryogenic fluids such as liquid nitrogen. These rules are intended to avert
two key risks: anoxia and burns from contact or splashes. Air contains 21% oxygen and 78% nitrogen, by volume. At
atmospheric pressure, liquid nitrogen evaporates at temperatures greater than –196°C.
In a cryogenic room, natural evaporation from recipients, filling, and handling of stored samples result in continuous
evaporation of liquid nitrogen. This can increase significantly if a fault occurs. If the room is not sufficiently well
ventilated, the nitrogen gas generated can cause the atmosphere to be depleted in oxygen.
Properties of nitrogen liquid
Does not sustain life or combustion.
Present in air (78%).
The evaporation of one litre of liquid nitrogen generates 680 litres of gas.
Heavier than air at low temperatures.
Non-flammable and colourless.
Ensuing risks:

Risk of asphyxiation and loss of consciousness. An atmosphere with less than 16% oxygen is hazardous (nitrogen gas

displaces oxygen in the air).

Nitrogen gas builds up in low areas.
Risk of frostbite.
Risk of corrosion of steels in the presence of humidity.
In closed vessels, the pressure can reach very high values (in the order of 700 bar) resulting in a risk of bursting.
Essential precautions to be taken
Areas where liquid nitrogen is stored or used must be well ventilated (extraction or ventilation at both high and low
levels in the room).
Areas liable to contain an unbreathable atmosphere must be indicated with an asphyxiation hazard pictogram.
Never enter a room that has contained liquid nitrogen without taking predefined precautionary measures.
Eliminate links between areas where liquid nitrogen is stored or used and low points (pits, drainage channels,
basements) where it could accumulate and render the atmosphere unbreathable.

Use an O2 concentration detector to ensure that the oxygen concentration is greater than 18%.

Avoid uninsulated liquid nitrogen pipes in any areas.
Call on the services of a specialist to determine the layout of facilities.
In the event of an incident or accident
If asphyxiation has occurred
Check the oxygen concentration in the room and then take the victim to the open air (taking an insufflator with you).
Begin artificial respiration and call the emergency services.
In the event of a leak
Evacuate the room.
Do not enter the room without breathing apparatus if the oxygen concentration is lower than 18%.
Close the valve on the leaking circuit.
Air the room at length, ensuring that low points are ventilated.
If liquid nitrogen is splashed:

In the eyes: Rinse the eye thoroughly for at least 20 minutes. Call a doctor.
On the skin: Do not rub. Remove clothing if necessary. Warm up the affected areas as quickly as possible under

running water for 20 minutes. Call a doctor.
PRECAUTIONS WITH REGARD TO EQUIPMENT
Warning: Nitrogen is liquid at extremely low temperatures and is stored in a double-walled tank. A high vacuum
between the walls ensures good thermal insulation. This equipment, especially mobile storage tanks, must be handled
with care.
Avoid impacts. Never lay a cryogenic container on its side.
Do not expose containers to excessive temperatures.
Treat valves with care (operate gently, do not dismantle or lubricate valves).
All equipment used must be in good condition and designed for use at the intended temperature and pressure.
Mobile equipment must be sheltered from inclement weather. Open-necked containers must be fitted with stoppers
to avoid the neck becoming blocked by cryopumping of ambient humidity.
Use suitable connection hoses; never use intermediate couplings.
Monitor the pressure of closed containers and check safety equipment (pressure relief valve).
Regulations apply to the transportation of recipients.
Users of the equipment, who are fully aware of the conditions of use and thus best placed to monitor the equipment,
are solely responsible for proper use.

Original manual – Iss. 0.1 User manual – BM 3E Freezer 44/ 44

14. CUSTOMER SERVICES

NORTHERN FRANCE
[e-mail]
Sales hotline

 [phone]
 [phone]

Technical hotline

 [phone]
 [phone]

SOUTHERN FRANCE
[e-mail]
Sales hotline

 [phone]
 [phone]

Technical hotline

 [phone]
 [phone]

EXPORT DIVISION
[e-mail]
Sales hotline

 [phone]
 [phone]

Technical hotline

 [phone]
 [phone]