Foss Soxtec 1047 User Manual

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Soxtec®1047 System

Hydrolyzing Unit

User Manual

1000 3315 / Rev. 2.2

1047011b

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1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

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1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

Table of Contents

1 PRECAUTIONS AND PROCEDURES

1.1 Intended use..............................................................................................1

1.2 Safety precautions.................................................................................1

2 INTRODUCTION

2.1 Performance data...................................................................................3

3 PREPARATIONS FOR USE

3.1 Unpacking and checking ....................................................................4

3.2 Installation .................................................................................................5

3.2.1 Installation requirements ............................................................................5

3.2.2 Fuses .................................................................................................................5

3.2.3 Installation instructions................................................................................5

4 OPERATION

4.1 Preliminary preparation.....................................................................6

4.2 Pre-extraction ...........................................................................................7

4.3 Hydrolysis ..................................................................................................8

4.4 Drying............................................................................................................9

4.5 Solvent extraction .................................................................................9

4.6 Calculation................................................................................................10

5 MAINTENANCE

5.1 Change of vacuum valve ................................................................11

5.2 Change of condenser .........................................................................11

5.3 Change of filter seal, upper ..........................................................11

5.4 Change of the lower thimble holder........................................12

5.5 Change of the vacuum tube ..........................................................12

5.6 Change of fuses.....................................................................................12

6 TECHNICAL SPECIFICATIONS

6.1 Safety...........................................................................................................13

6.2 Environmental conditions...............................................................13

6.3 Lifting instructions..............................................................................13

7 Foss Tecator APPLICATION NOTES SYSTEM 8 ACCESSORIES AND SPARE PARTS

8.1 Accessories ...............................................................................................15

8.2 Spare parts ...............................................................................................15

8.3 Manufacturer’s name and address............................................15

9 SAFETY INFORMATION 1047 (GB)................................17

9 SICHERHEITSINFORMATIONEN 1047(DE) ..............20

9 SIKKERHEDSINFORMATION 1047(DK) .....................24

9 INFORMACION DE SEGURIDAD 1047 (ES)............28

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

9 TURVALLISUUSOHJEET 1047 (FIN)................................32

9 INSTRUCTIONS DE SECURITE 1047(FR)....................36

9 ЛЗШЗ МЕФСЩН БУЦБЛЕЙБУ 1047 (GR)......................40

9 ÖRYGGISLEIÐBEININGAR 1047(IS)..............................45

9 INFORMAZIONI DI SICUREZZA 1047(IT).................48

9 VEILIGHEIDSINFORMATIE 1047(NL)...........................52

9 SIKKERHETSINFORMASJON 1047(NO) .....................56

9 INFORMAÇÕES DE SEGURANÇA 1047(PT)...........59

9 SÄKERHETSINFORMATION 1047(SE)..........................63

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

The 1047 Hydrolyzing Unit is designed for laboratory use analysing parameters as specified in Foss Tecators Application Notes.

Please read these operating instructions carefully and act accordingly. For safety reasons people not familiar with these operating instructions must not use the instrument.

2 INTRODUCTION

Crude Fat is generally defined as sample constituents soluble in appropriate organic solvents, such as diethyl ether, petroleum ether or chloroform. The main constituents in Crude Fat are triglycerols and free fatty acids. In certain Sample types, however, some of the fat, or lipids, are bound as lipoproteins, liposaccharides or metal salts. Solvents are not capable of breaking such bonds, therefore hydrolysis is used to transform these lipids into extractable forms.

The 1047 Hydrolyzing Unit in combination with Soxtec System HT enables total fat extractions to be performed in a simple way in a variety of Sample types.

2.1 Performance data

Sample size 0.5-5 g

Measuring range 0-100% fat

Capacity per batch 6 per unit

Speed of hydrolyzing cycle 20-60 min

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

STEC004A

Fig. 1 The procedure for fat determination with 1047 Hydrolyzing Unit and 1043 Soxtec Extraction Unit.

3 PREPARA TIONS FOR USE

3.1 Unpacking and checking

Unpack the 1047 Hydrolyzing Unit and its accessories with care. Remove all packing material. Use the parts list, packed together with the instrument and check that you have received all parts. Use Fig.2 to identify the different parts. If any parts are missing, contact your Foss Tecator representative.

1. Hydrolyzing Unit 2. Sample Tube (set of 6)

3. Glass Thimbles (set of 6) 4. Glass Thimble Holder (set of 6)

5. Water Spray Device 6. Beaker

7. Tube Stand 8. Reflector

9. Cleaning rod 10. Glass Thimble adaptor (set of 6)

11. Celite 12. Tube 8/11 L= 2 m

13. Tube 6/11 L= 2 m 14. Spray bottle, red

15. Cotton 16. Glass Thimble seals (set of 6)

17. Water Aspirator Pump 18. Hydrolysis Stand

19. Manual 22. Mains cable

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

1047011A

Fig. 2

3.2 Installation

All text in this section is translated in local language.

3.2.1

Installation requirements

Power supply 110 or 200-230V, 50-60 Hz

Power consumption 975W

Dimensions L xDxH 61x31x85cm

Weight 23 kg

Water supply Tap water approx. 2 l/min

(cooling). Tap water for vacuum pump.

Ventilation Fume hood is recommended.

Place the unit on the laboratory bench near suitable power, water and drainage.

3.2.2

Fuses

External: 230V: Two fuses T 6,3 A.

110V: Two fuses T 10 A.

3.2.3

Installation instructions

Before connecting to an earthed power supply, make sure that the mains voltage agrees with the voltage quoted at the rear of the unit.

Connect tubes to the rear of the unit according to fig. 3.

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

1047012A

1 2

VACCUM

WATER

DRAIN

Fig. 3 1. Equipotential screw, 2. Mains and fuses, 3. Vacuum (water aspirator pump), 4. Cooling water inlet, 5. Drain.

4 OPERATION

Total fat determinations according to most accepted procedures include the following main steps:

Hydrolysis with boiling acid

Cooling of solution with solubilized fat

Filtration

Drying of hydrolyzed sample residue

Solvent extraction

Drying of extracted matter

Weighing and calculation

Due to local reference methods, variations in e.g. acid strength, hydrolysis time, type of solvents etc. do occur.

Samples rich in fat (>10 %) are generally recommended to be pre-extracted before hydrolysis.

The following description of how to operate the 1047 Hydrolyzing Unit in conjunction with 1043 or 1045 Soxtec Extraction Systems, is generally based. For more detailed information on specific types of samples, please ask your local Foss Tecator dealer for "Application Notes".

4.1 Preliminary preparation

In order to achieve as good results as possible, all samples must be homogenized in a proper way before chemical treatment.

Sample Type of milI

Dry product, e.g. Cyclone mill e.g.

grains, foods, granulates

1093 Cyclotec.

Products high in moisture,

Knife homogenizer e.g.

fat or fibrous samples. 1094 Homogenizer or

Moulinex.

In order to make wet samples e.g. meat products more lyphilic, a short predrying before extraction is recommended.

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

4.2 Pre-extraction

As high fat levels can prevent effective hydrolysis, samples with 10% fat content should be pre-extracted with the same type of solvent as used in the final extraction.

1.Weight in the sample with a precision of

1000 ±2 mg. Load the glass thimble with 1 g celite and the sample. Do not cover with cotton.

2.Fit adapters on the thimbles, by introducing them into the thimbles in such a way that the circlip is compressed progressively into the thimble, (i.e. the closed side of the circlip is pressed in first and then working towards the open side), and insert them into the Soxtec HT Extraction Unit.

3.Add solvent and extract for 10 minutes in "Rinsing" position.

4.After the extraction, dry the extraction cups in an oven at 100°C for 30 minutes. Let them cool down and weigh them. Calculate percentage extracted fat. If the fat content is high, 20% , it is recommended to use a second extraction cup for the final extraction.

5.Remove the thimble holders from the glass thimbles. Transfer the pre-extracted sample as quantitatively as possible from the glass thimble to a sample tube for acid hydrolysis. Proceed from step 2 of the hydrolysis part.

Note: Use the same glass thimbles for the filtration after hydrolysis (see 4.3 position 4 )

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

1047013A

Fig. 4 Insert the tube with sample and celite into the Hydorlyzing Unit.

4.3 Hydrolysis

1. Weigh the samples with a precision of

1000 ±2 mg and transfer them to the sample tubes.

2. Add 1-2 g of celite and 100-120 ml of the acid solution to each sample tube. The level of the acid solution must be over the top of the cover of the heaters.

3. Insert the tubes into position in the 1047 Hydrolyzing Unit (Fig. 4). Lower the suction tubes to the fume exhaust position by pulling the handle upwards (Fig. 5). The teflonpart should rest on the top of the sample tubes and the suction tubes should be about one centimeter down in the sample tubes.

4. Place the glass thimbles into the thimble supports (Fig. 6) and insert them into the Hydrolyzing Unit(Fig. 7).

Note: If pre-extraction according to section 4.2 has been carried out, check that the thimbles are used for the same samples as in pre-extraction.

5. Start the water aspirator pump for the fume exhaust system. Open vacuum valves (Fig. 7) under each thimble. Adjust during boiling for low fume exhaust.

6. Turn on the heater to maximum effect and place the reflector in front of the sample tubes. When the solution starts boiling, adjust to a gently boiling speed with the heater control (Fig. 8).

7. At the end of the hydrolysing period, turn off the heater and remove the reflector.

8. Open the cold water tap for the condensers (approximately 2 l/m).

9. Pull down the suction tube handle to pos. 1 (Fig. 5). Dilute the acid solution

in each tube with some 100 ml of 20-25°C distilled water. Close the vacuum valve (Fig. 7) under each thimble.

10. Lower the suction tubes to pos. 2 (Fig. 5). Open the vacuum valve (Fig. 7) under one thimble and suck up the sample solution and as much as possible of the solid particles in the solution. Close the valve and repeat in the same way with the rest of the tubes.

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

1047014A

Fig. 5 Pull the handle up (to pos 2) to lower the suction tubes.

1047015A

Fig. 6 Use gloves when handling thimbles to minimize contamination.

1047016A

Fig. 7 Turn the knob to open/close the vacuum valve.

11. Wash each tube by opening the vacuum valve and spray 5x50 ml of approximately 50°C distilled water, by using the water sprayer (Fig. 9).

12. Raise the suction tubes and take out one of the sample tubes. Cover the cleaning rod with a thin layer of defatted cottonwool and wet it with acetone. Clean the inside of the tubes by gently pushing and pulling the cleaning rod up and down in the tube (Fig. 10). Take a small piece of the cottonwool and clean the outside of the suction tube if necessary. Place each pad of the cottonwool on the top of the sample residue in the glass thimble.

Note: Do not have the valves in closed position when the Hydrolysing Unit is shut down.

4.4 Drying

To make the wet sample residue lyophilic and achieve effective solvent extraction, the residue and thimble must be dried.

Alt. 1: Dry overnight at 60-80°C in a oven. Alt. 2: Dry in a microwave oven with

turntable at medium power for 30-60 minutes.

Most samples can be dried in a microwave oven, except meat and fish products.

WARNING!

Do not use a microwave oven if residues of organic solvents are present.

4.5 Solvent extraction

1.Fit the adapters, as written in 4.2:2, to

each thimble and insert them into the Soxtec HT Extraction Unit.

2.Extract and analyse the samples according

to the Soxtec HT Extraction Unit instructions, see Soxtec HT manual.

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

1047017A

Fig. 8

1047018A

Fig. 9 Wash the tubes with the sprayer to suck up the residues of the sample solution.

1047019A

Fig. 10 Clean the tube with the cleaning rod and defatted cotton.

4.6 Calculation

Calculate the fat content according to the formula below.

W3 = The extraction cup weight after fat

extraction and cooling in desiccator.

W2 = Clean and predried extraction cup.

W1 = Sample weight.

% Fat =

W3-W2

x 100

If a pre-extraction was required, using two extraction cups, add the two results that have been calculated, and the sum is the total fat in the sample.

F1 = Fat content after pre-extraction. F2 = Fat content in final extraction.

% Fat/Oil = F1 + F2

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

5 MAINTENANCE

5.1 Change of vacuum valve

1. Loosen all neoprene tubings carefully that are connected to the suction tubes, by removing the plastic straps.

2. Lower the suction tubes down the horizontal plate, so the suction tubes rest against the bottom of the oven.

3. Unscrew the 3 screws that are placed on the white plate on the backside of the hydrolyzing unit. Unscrew the 3 screws that are on the bottom of the white plate.

4. Take away the white plate.

5. Loosen the 6 metal clips on the neoprene tubings connected to the connection tube and then loosen the neoprene tubes from the connection tube.

Note: Do not take away the neoprene tube that is situated to the far right.

6. Fold the connection tube to the right.

7. Carefully loosen and take away the filter seal that is connected on the top of the vacuum valve to be changed.

8. Turn the vacuum valve knob counter clockwise until it disengages.

9. The vacuum valve is now free and is drawn out from the rear of the hydrolyzing unit.

10. Fit the new vacuum valve and put together in reverse order.

5.2 Change of condenser

1. Take away the plastic straps on the 3 neoprene tubings that are

connected to the condenser and loose the tubings from the condenser.

2. Loosen carefully the condenser from the filter seal and pull the

condenser right up.

3. Assemble the new condenser and connect the tubes in the same

way as for the old condenser.

5.3 Change of filter seal, upper

1. Loosen the condenser according to paragraph 5.2 Pos. 1-3

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

2. Carefully loosen and pull out the old filter seal. Press gently down the new filter seal.

Note: Check carefully the correct positioning of the new filter seals.

5.4 Change of the lower thimble

holder

1. Loosen the old thimble holder by gently easing and pulling it upwards.

2. Press down the new thimble holder and check that it fits correctly.

5.5 Change of the vacuum tube

1. Remove the plug from the wall socket.

2. Unscrew the instrument panel with the heating control knob.

3. Remove the white plate on the back of the hydrolyzing unit according to paragraph 5.1 Pos. 1- 4.

4. Remove the plastic strap on the neoprene tube that is situated to the far right on the connection tube.

5. Unscrew the nipple on the back of the hydrolyzing unit that is marked "vacuum".

6. Pull out the tube with the nipple through the instrument hole.

7. Cut the new vacuum tube to 450 mm and assemble the nipple from the old tube.

8. Assemble the rest in reverse order.

5.6 Change of fuses

1. The 2 pole fuse holder is located under the mains cable. Push the middle button and change the fuses.

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

1047012A

1 2

VACCUM

WATER

DRAIN

Fig. 11 Pos 2: 2 pole fuse holder and mains

6 TECHNICAL

SPECIFICATIONS

6.1 Safety

The insulation of external, inaccessible circuits is reinforced.

6.2 Environmental conditions

The equipment is designed to be safe at least under the following conditions:

Indoor use

Altitude up to 2000 m.

Temperature 5°C to 40°C.

Maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.

Mains supply voltage fluctuations not exeeding ±10% of the rated voltage.

Transient overvoltage is according to category II, which is normal for this type of equipment.

Pollution degree 2.

6.3 Lifting instructions

This instrument is not equipped with handles or grips. One or two persons are needed to lift the instrument, following the instructions below:

1. Tilt the instrument slightly backwards, lift and support the instrument as shown in fig.12 to simplify and ensure a safe lift.

2. Weight 23 kg.

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

1047021a

Fig. 12

7 Foss Tecator

APPLICATION NOTES SYSTEM

For information about preparation of chemicals and the Solvent Extraction (with hydrolysis) method. please see the Application Note AN 301.

In Foss Tecators extensive list of Application Sub Notes you will find information on how to analyse the sample of your choice. Please contact your Foss Tecator representative for more details.

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

Fig. 13

8 ACCESSORIES AND

SPARE P AR TS

8.1 Accessories

Order no.

Tube Stand 1000 0012

Digestion Tubes (set of 6) 1000 0155

Glass Thimble Holder (set of 6) 1000 3218

Glass Thimble (set of 6) 1000 3217

Cleaning Rod 1000 3275

Stand for 6 glass thimbles (stainless steel) 1000 3640

Stand for 6 glass thimbles (plastic) 1000 3669

Glass Thimble Adapter 1000 6630

Spray Bottle (red) 1522 0006

Beaker 1522 0014

Cotton (Defatted) 1529 0009

Water Aspirator Pump 1529 0021

8.2 Spare parts

Vacuum valve 1000 3200

Condenser 1000 3209

Filter seal, upper, set of 6 1000 3219

Lower Thimble Holder, set of 6 1000 3343

Vacuum Tube, Viton Tubing 12/16 mm 1582 0017

Glass Suction Tube 1000 3207

Heating Oven, 230 V 1000 3220

Heating Oven, 110 V 1000 3230

Indication Lamp, 230 V 1564 0037

Indication Lamp, 110 V 1564 0065

Potentiometer 1566 0034

Heating Element, 230 V 1578 0008

Heating Element, 110 V 1578 0009

Neoprene Tubing, 6x12 1582 0023

Tube clamp, dia 14 mm 1582 0051

Tube clamp, dia 16 mm 1582 0052

Mains cable 230V 1565 0026

Mains cable 110V 1565 0063

Fuse T 6,3 A 1564 0083

Fuse 10 A 1564 0006

8.3 Manufacturer’s name and address

Foss Tecator AB Box 70 S-263 21 Höganäs Sweden Int phone. +46 42 361500 Int fax. +46 42 340349

1047 Soxtec System Hydrolyzing Unit, User Manual, Rev. 2.2, Part no 1000 3315, Foss Tecator AB, Sweden

9.4.2 Pre-extraction

As high fat levels can prevent effective hydrolysis, samples with 10 % fat content should be pre-extracted with the same type of solvent as used in the final extraction.

1. Weight in the sample with a precision of 1000 ±2 mg. Load the glass thimble with 1 g celite and the sample. Do not cover with cotton.

2. Fit adapters on the thimbles, by introducing them into the thimbles in such a way that the circlip is compressed progressively into the thimble, (i.e. the closed side of the circlip is pressed in first and then working towards the open side), and insert them into the Soxtec HT Extraction Unit.

3. Add solvent and extract for 10 minutes in "Rinsing" position.

4. After the extraction, dry the extraction cups in an oven at 100°C for 30 minutes. Let them cool down and weigh them. Calculate percentage extracted fat. If the fat content is high, 20% , it is recommended to use a second extraction cup for the final extraction.

5. Remove the thimble holders from the glass thimbles. Transfer the pre-extracted sample as quantitatively as possible from the glass thimble to a sample tube for acid hydrolysis. Proceed from step 2 of the hydrolysis part.

Note: Use the same glass thimbles for the filtration after hydrolysis (see 9.4.3 Pos. 4)

9.4.3 Hydrolysis

1. Weigh the samples with a precision of 1000 ±2 mg and transfer them to the sample tubes.

2. Add 1-2 g of celite and 100-120 ml of the acid solution to each sample tube. The level of the acid solution must be over the top of the cover of the heaters.

4. Place the glass thimbles into the thimble supports (Fig. 6) and insert them into the Hydrolyzing Unit (Fig. 7).

Note: If pre-extraction according to section

9.4.2 has been carried out, check that the

thimbles are used for the same samples as in pre-extraction.

5. Start the water aspirator pump for the fume exhaust system. Open vacuum valves (Fig.

7) under each thimble. Adjust during boiling for low fume exhaust.

6. Turn on the heater to maximum effect and place the reflector in front of the sample tubes. When the solution starts boiling, adjust to a gently boiling speed with the heater control (Fig. 8).

7. At the end of the hydrolysing period, turn off the heater and remove the reflector.

8. Open the cold water tap for the condensers (approximately 2 1/m).

9. Pull down the suction tube handle to pos. 1 (Fig. 5). Dilute the acid solution in each tube with some 100 ml of 20-25°C distilled water. Close the vacuum valve (Fig. 7) under each thimble.

10. Lower the suction tubes to pos. 2 (Fig. 5). Open the vacuum valve (Fig. 7) under one thimble and suck up the

sample solution and as much as possible of the solid particles in the solution. Close the valve and repeat in the same way with the rest of the tubes.

11. Wash each tube by opening the vacuum valve and spray 5x50 ml of approximately 50°C distilled water, by using the water sprayer (Fig. 9).

Note: Do not have the valves in closed position when the Hydrolysing Unit is shut down.

9.4.4 Drying

To make the wet sample residue lyophilic and achieve effective solvent extraction, the residue and thimble must be dried.

Alt. 1: Dry overnight at 60-80°C in a oven. Alt. 2: Dry in a microwave oven with turntable

at medium power for 30-60 minutes.

Most samples can be dried in a microwave oven, except meat and fish products.

Warning

Do not use a microwave oven if residues of organic solvents are present.

9.4.5 Solvent extraction

1. Fit the adapters, as written in 9.4.2:2, to each thimble and insert them into the Soxtec HT Extraction Unit.

2. Extract and analyse the samples according to the Soxtec HT Extraction Unit instructions, see Soxtec HT manual.

9.4.6 Calculation

Calculate the fat content according to the formula below.

W3 = The extraction cup weight after fat

extraction and cooling in desiccator.

W2 = Clean and predried extraction cup.

W1 = Sample weight.

% Fat =

W3-W2

x 100

If a pre-extraction was required, using two extraction cups, add the two results that have been calculated, and the sum is the total fat in the sample.

F1 = Fat content after pre-extraction.

F2 = Fat content in final extraction.

% Fat/Oil = F1 + F2

9.5 MAINTENANCE

9.5.1 Change of vacuum valve

1. Loosen all neoprene tubings carefully that are connected to the suction tubes, by removing the plastic straps.

2. Lower the suction tubes down the horizontal plate, so the suction tubes rest against the bottom of the oven.

1047 Instruction Manual, Rev. 2.2 10/00, 1000 3315 18

3. Unscrew the 3 screws that are placed on the white plate on the backside of the hydrolyzing unit. Unscrew the 3 screws that are on the bottom of the white plate.

4. Take away the white plate.

5. Loosen the 6 metal clips on the neoprene tubings connected to the connection tube and then loosen the neoprene tubes from the connection tube.

Note: Do not take away the neoprene tube that is situated to the far right.

6. Fold the connection tube to the right.

7. Carefully loosen and take away the filter seal that is connected on the top of the vacuum valve to be changed.

8. Turn the vacuum valve knob counter clockwise until it disengages.

9. The vacuum valve is now free and is drawn out from the rear of the hydrolyzing unit.

10. Fit the new vacuum valve and put together in reverse order.

9.5.2 Change of condenser

1. Take away the plastic straps on the 3 neoprene tubings that are connected to the condenser and loose the tubings from the condenser.

2. Loosen carefully the condenser from the filter seal and pull the condenser right up.

3. Assemble the new condenser and connect the tubes in the same way as for the old condenser.

9.5.3 Change of filter seal, upper

1. Loosen the condenser according to paragraph 9.5.2 Pos. 1-3

2. Carefully loosen and pull out the old filter seal. Press gently down the new filter seal.

Note: Check carefully the correct positioning of the new filter seals.

9.5.4 Change of the lower

thimble holder

1. Loosen the old thimble holder by gently easing and pulling it upwards.

2. Press down the new thimble holder and check that it fits correctly.

9.5.5 Change of the vacuum tube

1. Remove the plug from the wall socket.

2. Unscrew the instrument panel with the heating control knob.

3. Remove the white plate on the back of the hydrolyzing unit according to paragraph

9.5.1 Pos. 1- 4.

4. Remove the plastic strap on the neoprene tube that is situated to the far right on the connection tube.

5. Unscrew the nipple on the back of the hydrolyzing unit that is marked "vacuum".

6. Pull out the tube with the nipple through the instrument hole.

7. Cut the new vacuum tube to 450 mm and assemble the nipple from the old tube.

8. Assemble the rest in reverse order.

9.5.6 Change of fuses

1. The 2 pole fuse holder is located under the mains cable. Push the middle button and change the fuses.

9.6 TECHNICAL SPECIFICATIONS

9.6.1 Safety

The insulation of external, inaccessible circuits is reinforced.

9.6.2 Environmental conditions

The equipment is designed to be safe at least under the following conditions:

Indoor use

Altitude up to 2000 m.

Temperature 5°C to 40°C.

Maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.

Mains supply voltage fluctuations not exeeding ± 10% of the rated voltage.

Transient overvoltage is according to category II, which is normal for this type of equipment.

Pollution degree 2

9.6.3 Lifting instructions

This instrument is not equipped with handles or grips. One or two persons are needed to lift the instrument, following the instructions below:

1. Tilt the instrument slightly backwards, then

lift and support the instrument as shown in fig.12 to simplify and ensure a safe lift.

2. Weight 23 kg.

9.6.4 Explanations to

illustrations

Fig. 3

1. Equipotential screw

2. Mains and fuses

3. Vacuum (water aspirator pump)

4. Cooling water inlet

5. Drain

Fig. 4 Insert the tube with sample and celite into the Hydrolyzing Unit.

Fig. 5 Pull the handle up (to pos 2) to lower the suction tubes.

Fig. 6 Use gloves when handling thimbles to minimize contamination.

Fig. 7 Turn the knob to open/close the vacuum valve.

Fig. 8 Adjust heating with the heating control.

Fig. 9 Wash the tube with the sprayer to suck up the residues of the sample solution.

Fig. 10 Clean the tube with the cleaning rod and defatted cotton.

Fig. 11 Pos 2: 2 pole fuse holder and mains

1047 Instruction Manual, Rev. 2.2 10/00, 1000 3315 19

Foss Soxtec 1047 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual