Fein Optic RB50 User Manual

RB50 Microscope User’s Manual

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Microscope Components:

RB50 Fluorescence Microscope

Eyepieces

Filter Cube

Selector

Sextuple Nosepiece

Mechanical Stage

Shutter Lever

Trinocular Port

Beam Splitter

Field Diaphragm

Adjustment

Aperture Diaphragm

Adjustment

Hex Wrench

Diaphragm Centering

Screws

Y-Axis Knob

X-Axis Knob

Condenser

Illuminator

Light Rheostat

Adjustment

Knob

Focusing Tension

Page 1

Voltage Indicator

Reset Button

for Intensity

Coarse Focusing

Fine Focusing

Adjustment

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Microscope Components:

RB50 Fluorescence Microscope

Fluorescence Illuminator

Attachment

100w Halogen

Light Source

ND Filter Slots

Quintuple
Nosepiece

Coarse Adjustment

Limit Ring

Filter Levers

Page 2

Coarse Focus

Knob

Fine Focus

Knob

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Binocular / Trinocular Microscope Assembly:

RB50 Microscope Assembly

RB50 Fluorescence Microscope Assembly

Page 3

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Before Use:

Do not shake or drop the microscope.

Do not not expose the microscope to
direct sun, high temperatures, dust, or
damp environments. Use a at work
surface. Indoor operating temp
41°~104°F (5°~40°C), max relative
humidity of 80%.

When moving the microscope use both hands,
holding by the handle at the back (1) and the base
(2) as shown at right.

When working, the surface of the light source
will be warm. Make sure there is enough room
for the heat to dissipate around the light
source.

Set the power switch to o “O” before
replacing a bulb or fuse, and wait until the
lamp is cool. The power switch (3) is located on
the back of the base of the microscope.
Microscope uses 12V, 100W halogen bulb.
(Part# 12v100wH).

Voltage range of 100~240V is supported.
Additional transformer is not necessary. Use
only an outlet with voltage in this range and
use the power cord supplied with the
microscope.

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Maintenance:

Wipe lenses gently with a soft tissue. Carefully remove excess oil from the 100x immersion oil lens.
Wipe o ngerprints from lens surfaces with lens paper using a small amount of microscope cleaning
solution or a 3:7 mixture of alcohol and ether or dimethylbenzene. (Alcohol and ether are ammable,
do not place these chemicals near re and clean in a ventilated area.)

When cleaning other surfaces of the microscope use water only. A basic detergent can be used to
clean the surface if necessary, but ensure that all the detergent is removed from the frame with a
clean, damp cloth prior to drying the surface.

If the microscope becomes wet during use, power off the microscope and dry the microscope
thoroughly.

Do not disassemble the microscope.

After use, cover the microscope with a dust cover and power o the light.

Objectives:

RB50 Innity Corrected Objectives all have a parfocal distance of 45mm.
Recommended coverslip thickness is 0.17mm.

Objective Type

Plan Achromat

Plan Semi

Apochromat Fluor

Plan Phase

Contrast

Part # / Magnication

FPLN4 / 4x

FPLN10 / 10x

FPLN20 / 20x

FPLN40 / 40x

FPLN50 / 50x Oil

FPLN60 / 60x

FPLN100 / 100x Oil

SAPOFL4 / 4x

SAPOFL10 / 10x

SAPOFL20 / 20x
SAPOFL40 / 40x

SAPOFL100 / 100x Oil

FPL-PH10 / 10x
FPL-PH20 / 20x

FPL-PH40 / 40x

FPL-PH100 / 100x Oil

Numerical Aperture

0.10

0.25

0.40

0.65

0.95

0.85

1.25

0.13

0.30

0.50

0.75

1.28

0.25

0.40

0.65

1.25

Working Distance

11.9mm

12.1mm

1.5mm

0.36mm

0.19mm

0.3mm

0.18mm

18.5mm

10.6mm

2.33mm

0.6mm

0.21mm

12.1mm

1.5mm

0.36mm

0.18mm

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Step-by-Step Assembly:

Light Source Assembly & Bulb Replacement:
Loosen the lock screw (1) completely with an M4
spanner and remove the cover.
Open the bulb lock (2).
Handle the bulb (3) with a clean glove or soft tissue
(do not touch the bulb with ngers).
Insert the bulb pins (4) into the bulb holder (5).
The bulb should be vertical after assembly.
If any ngerprints got on bulb, wipe clean with a
clean, soft cloth.
Replace bulb only with 12v, 100w halogen bulb
(part# 12v100wH).
Before replacing the bulb, make sure to unplug and
power o the microscope. Wait for the bulb housing
to cool o before opening.

Light Source Microscope Assembly:
Push the light source holder (1) into the body of the
microscope. Keep the light source horizontal in
relation to the microscope body and tighten the
screw (2).

Stage Assembly:
Loosen the set screw (1) on the stage.
Center the stage on the base, aligning the two “V”
notches on the bottom of stage and on the
condenser (2).
Tighten the set screw.

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