No part of this publication may be reproduced, transmitted, transcribed, stored in a
retrieval system or translated into any language in any form by any means without the
prior written consent of Excelitas Canada Inc.. Every effort has been made to ensure
information in this manual is accurate; however, information in this manual is subject to
change without notice and does not represent a commitment on the part of the authors.
Excelitas Canada Inc.
2260 Argentia Road
Mississauga ON L5N 6H7 CANADA
Trademarks
X-Cite®, Intelli-Lamp®, and Closed-Loop Feedback are registered trademarks of Excelitas
Canada Inc.. All rights reserved.
All other product names are trademarks of their respective owners.
The lamp is warming up. Warm up time is
approximately 4 minutes ref section 6.1
bulb
Lamp Error
Lamp installed incorrectly /Lamp did not strike.
See section 8.3
cool
Lamp is too hot to strike
The lamp will automatically strike when it has
cooled to the optimum striking temperature.
See section 8.3
Alternating:
old / bulb
Old Lamp
The lamp has accumulated over 2500 hours.
Lamp may be near end of life.
Alternating:
end / bulb
New Lamp Required
The lamp has reached end of life. The lamp
will not strike.
SFI
Shutter Failure
The shutter has failed to return to home
position. Unit should be restarted. If the error
repeats, contact Tech Support.
E1
Lamp temperature is too high
Check ventilation filters and outlets to ensure
that no blockage exists. Restart unit and if
problem persists replace lamp. If problem
continues contact Tech Support
E4
Internal hardware failure
Restart unit, if problem persists contact Tech
Support
E5
Failure to communicate with
Intelli-Lamp
Check Intelli-Lamp connection and restart unit.
If problem persists try another lamp, if problem
still persist contact Tech Support.
E6
Lamp self extinguished
Let system cool and restart lamp. If it goes out
again, replace lamp. If new lamp continues to
self-extinguish, contact Tech Support
E7
Internal system error
Restart unit, if problem persists contact Tech
Support
1 X-Cite® 200DC Message Reference
See Section 8 for troubleshooting.
X-Cite® 200DC User Guide
035-00446R rev. 4
1
2 Introduction
The X-Cite® 200DC illuminator is the ultimate combination of ease of use and
performance. Like the previous X-Cite® illuminators, the X-Cite® 200DC has the
convenience of pre-aligned, long-life Intelli-Lamps, easy installation and compatibility
with all major microscope brands. With smooth 0-100% intensity control, a high speed
shutter and ultra-stable lamp output, the X-Cite® 200DC is an ideal choice for both
routine assays and advanced imaging applications.
Excelitas Technologies Corp. is a global technology leader focused on delivering
innovative, customized solutions to meet the lighting, detection and other highperformance technology needs of customers. X-Cite®, formerly of Lumen Dynamics
(which was acquired by Excelitas Technologies Corp. in November 2013) offers the Life
Science and Analytical Instrumentation market a broad range of innovative lamp and
LED fluorescence illumination and measurement solutions.
The X-Cite® 200DC is a high quality product manufactured in accordance with ISO 9001,
CE marked and certified to UL, China RoHS and CSA standards.
We suggest that you read this manual to discover all its features, and how to use them.
Thank you for choosing X-Cite®!
X-Cite® 200DC User Guide
035-00446R rev. 4
2
3 Safety
3.1 Glossary of Symbols
CAUTION - Risk of danger: consult accompanying documents
WARNING -Eye damage may result from directly viewing ultraviolet light –
protective eye shielding and clothing must be used at all times.
Input/Output Signals
Input Signal
CAUTION – Hot Surface
3
X-Cite® 200DC User Guide
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3.2 Safety Precautions
Please observe the following precautions during use. This series of cautions,
warnings and dangers relate to the operation and maintenance of the
X-Cite® 200DC. They are also presented throughout this User’s Guide where
necessary.
Warning
Eye damage may result from directly viewing the light produced by the lamp used
in this product. Always use protective eyewear and turn the lamp off before
removing cover.
Caution
Never look into the light emitting end of the light guide. The light could severely
damage the cornea and retina of the eye if the light is observed directly. Eye
shielding must be used at all times as well as clothing to protect exposed skin.
Warning
Always make sure the light guide is properly inserted into the X-Cite® 200DC and
the microscope prior to turning on power to the unit. This will minimize the risk of
exposure to the UV light.
Caution
UV emitted from this product. Eye or skin irritation may result from exposure.
Use appropriate shielding.
Warning
IR emitted from this product. Do not stare at operating lamp.
Caution
Possibly hazardous optical radiation emitted from this product. Do not stare at
operating lamp. May be harmful to the eyes.
Caution!
To prevent damage/ degradation of the light guide, always allow adequate
clearance at the front of the X-Cite® 200DC unit to prevent kinking or excessive
bending.
Warning
To reduce the risk of fire or shock, always replace the fuses with the same type
and rating.
Warning
Disconnecting of main supply source is only possible by unplugging the power
cord.
X-Cite® 200DC User Guide
035-00446R rev. 4
4
Danger
This unit contains HIGH VOLTAGE components. It is recommended that ONLY
QUALIFIED TECHNICAL PERSONNEL perform any testing or repairs described in
this manual. Disconnect the AC power cord from the unit before opening the cover
of this unit. All cover screws must be replaced prior to applying power to the unit, or
safety of the unit will be impaired.
Monitoring the unit during manual operation
The level of UV and visible energy supplied by the X-Cite® 200DC is sufficient to
ignite flammable substances. During manual operation, the unit must be attended
at all times by a qualified operator. The unit must not be left unattended while
turned on. If an operator leaves the work area of the unit, the lamp power switch
must be turned off.
Monitoring the unit during Automated operation
The level of UV and visible energy supplied by the X-Cite® 200DC is sufficient to
ignite flammable substances. Therefore, when the unit is operated unattended in
an automated environment, an alarm function must be provided by the user to
indicate a malfunction in the associated equipment used.
Warning
Hg – LAMP CONTAINS MERCURY, Manage in Accord with Disposal Laws,
see: www.lamprecycle.org or 1-800-668-8752
Danger: Exposure to Mercury represents a health hazard to humans.
When unpacking or installing the lamp, always wear protective clothing and a face
mask. Operate the lamp inside the X-Cite® 200DC housing only. This prevents
direct viewing of the arc and in the case of lamp bursting, contains the lamp
particles. In the rare instance in which a lamp bursting occurs, and the mercury
content is released, the following safety precautions are recommended; all
personnel should be immediately evacuated from the area to prevent inhalation of
the mercury vapour. The area should be well ventilated for a minimum of 30
minutes. Prior to clean up ensure an ‘approved-for-mercury’ respirator mask and
non-porous gloves such as latex or rubber are used. After the lamp housing
elements have cooled, the mercury residue should be collected with the use of a
special absorbing agent available from laboratory equipment suppliers.
Listed below are examples of internet web sites for obtaining Mercury Spill Kits;
Should this X-Cite® 200DC unit be used in a manner not specified by Excelitas
Technologies, the protection provided by the equipment may be impaired.
X-Cite® 200DC User Guide
035-00446R rev. 4
5
Warning
The method in which lamps are disposed of must comply with local rules and
regulations for disposal of hazardous materials. Lamps may be returned to
Excelitas Technologies providing they are returned in their original packaging.
Excelitas Technologies will dispose of them in the appropriate manner.
Warning
This unit is designed for bench top use only! Always ensure that the unit is
operated on a hard, stable surface. This will prevent obstruction of the bottom
chassis ventilation openings. Any obstruction of these openings could result in a
possible over-heating condition. Do not attempt to remove or tamper with the
rubber feet located on the bottom of the unit.
Caution
The lamp module’s operational life can be significantly shortened if it is handled
incorrectly. Do not touch the bulb’s glass envelope or the inside surface of the
reflector. Skin oils can cause the lamp module to fail prematurely.
Caution
Prior to opening the unit and handling the lamp module, allow a minimum of 5
minutes for the lamp module to cool down completely.
Caution
Any electronic equipment connected to the X-Cite® 200DC must be comply with
the requirements of EN/IEC 60950.
Cleaning:
Clean the exterior of the unit with a slightly dampened cloth and simple water/
detergent solution only.
Carefully unpack the unit and accessories. Ensure that all components are present.
Store the packing material for future use.
Box Contents:
If your packaged unit is missing any of the above components,
call Excelitas Canada Inc, at (905) 821-2600
or TOLL FREE 1-800-668-8752.
Any additional optional items purchased to customize the unit will also be present.
X-Cite® 200DC User Guide
035-00446R rev. 4
7
LED DISPLAY
SHUTTER
ACTIVATION
BUTTON
LAMP “ON”
STATUS LED
SHUTTER
STATUS LED
IRIS
ADJUSTMENT
DIAL
LIGHT GUIDE
PORT
LIGHT GUIDE
STATUS LED
POWER ON/OFF
SWITCH
4.2 Front Panel
Figure 1 Front Panel
8
X-Cite® 200DC User Guide
035-00446R rev. 4
AC RECEPTACLE
AND FUSE
DRAWER
FOOT PEDAL
INPUT
TTL OUTPUT
FOR SHUTTER
SYNC
TTL INPUT FOR
SHUTTER
4.3 Rear panel
Figure 2 Rear Panel
9
X-Cite® 200DC User Guide
035-00446R rev. 4
Caution! The lamp module’s operational life can be significantly shortened if
handled incorrectly. Be sure only to handle the ceramic surfaces and
the lamp rim. Do not touch the bulb’s glass envelope or the inside
surface of the reflector. Skin oils can cause the lamp module to fail
prematurely.
LAMP HOUSING
SIDE PANEL
Note: Review Section 2 – Safety Precautions before proceeding
5 Installation/Set-up
5.1 Installing Lamp Module
5.1.1 Be sure the AC POWER cord is disconnected from the unit.
5.1.2 Remove the screw from the lamp housing side panel using the 3mm hex tool
provided and remove the panel from the unit cover.
Figure 3 Side Panel
5.1.3 Carefully remove the lamp module from its container, holding only the ceramic
component and lamp rim.
5.1.4 As illustrated below, position the lamp facing towards the front of the unit with the
POWER leads facing towards you. The lamp should be aligned so that the
leading edge of the reflector (lamp rim) fits into the mounting groove on the lamp
holder assembly.
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10
SPRING CLAMP
CERAMIC
COMPONENT
POWER
LEADS
LAMP RIM
LAMP HOLDER
Figure 4 Lamp Housing
5.1.5 Make sure the middle of the lamp is in position to fit into the spring clamp. Slide
the lamp until it snaps into the spring clamp. The leading edge of the reflector
(lamp rim) should fit snugly into the lamp holder recess.
11
X-Cite® 200DC User Guide
035-00446R rev. 4
CENTER BAR
AND LEAD
WITHIN CUT-OUT
POWER
CONNECTOR
INTELLI-LAMP
CONNECTOR
5.1.6 Locate the 4-pin Intelli-Lamp sensor connector at the rear of the lamp module
and connect it to its mate located on the top of the lamp-housing wall.
Tip: The Intelli-Lamp connector will only attach in the correct orientation. If you
are having difficulty attaching the connector, try rotating it by 180º.
Note: If the Intelli- Lamp connector is not installed correctly, the lamp will not
strike and the “bulb” message will display when POWER is turned on to
the unit.
Figure 5 Lamp connections
5.1.7 Locate power connector with two leads and connect it to its mate located on the
side of the lamp-housing wall.
12
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Warning This unit is designed for bench top use only! Always ensure that the
unit is operated on a hard, stable surface. This will prevent
obstruction of the bottom chassis ventilation openings. Any
obstruction of these openings could result in a possible over-heating
condition. Do not attempt to remove or tamper with the rubber feet
located on the bottom of the unit.
5.1.8 Ensure the lamp anode cooling fin (bar) and lamp power lead, at the front of the
lamp, are centered within the lamp holder cut-out. Rotate the lamp as required.
5.1.9 Replace the lamp housing side panel and tighten the fastening screw.
Note: If the lamp housing panel is not secured completely the lamp will not strike
and the “bulb” message will display when power is turned on to the unit.
5.1.10 Replace the hex tool in the clips underneath the system for future use.
5.2 Inserting/Removing Light Guide
5.2.1 Ensure the power is not connected to the X-Cite® 200DC unit.
5.2.2 Ensure that the protective end caps are removed from both the input and output
ends of the light guide prior to installation.
5.2.3 Remove plastic plug (if present) from the light guide port on the front panel of the
X-Cite® 200DC.
5.2.4 Holding the light guide by the grey sleeve and strain relief portion only, insert the
light guide into the light guide port. Push the light guide in until it seats with a
second positive "click" (up to the “A” line printed on the grey ferrule).
Note: Never grip the light guide during installation or removal in a place other
than the reinforced strain relief portion of the light guide.
X-Cite® 200DC User Guide
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13
LIGHT GUIDE
INSERTION LINE
GREEN LIGHT GUIDE
STATUS LED
LIGHT GUIDE
INSERTION LINE
RED LIGHT GUIDE
STATUS LED
Figure 6 Light Guide Position
Tip: When the light guide is fully inserted, the line on the light guide insertion
label will be right up against the light guide port. Also, the LED above the
light guide port will illuminate green when POWER is on to the unit. If the
light guide is not fully inserted the LED will illuminate red.
Note: The shutter will not open if the light guide is not fully inserted.
5.2.5 To remove the light guide, firmly grip the strain relief near the light guide retainer
and pull out firmly.
Note: The X-Cite® 200DC is designed for use with a 3mm liquid light guide.
Excelitas Technologies can not guarantee the performance of the X-Cite®
200DC if using light guides other than those supplied by Excelitas
Technologies.
Note: The light guide has a minimum bend radius of 1.6 inches (40.mm).
Bending or coiling the light guide tighter than this radius will result in
permanent damage to the light guide.
14
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035-00446R rev. 4
5.3 Installing the Collimating Adapter
5.3.1 Ensure that the power is off on the X-Cite® 200DC unit.
5.3.2 The existing lamp housing must be removed from the microscope before the
collimating adapter can be mounted. Do not discard the existing mounting
hardware, since it will be used to secure the collimating adapter to the
microscope.
5.3.3 Remove the collimating adapter from its packaging.
Note: The collimating adapter has been factory set. No adjustments by the
customer are required; however some adapter models are outfitted with
centering set screws, for longer light path applications. Separate
instructions are included with these models.
5.3.4 Insert the flange portion of the collimating adapter into the lamp port of the
microscope. Using the existing hardware, tighten the screw(s) until the flange is
fully secured.
Note: The installation of the collimating adapter will vary with microscope
models.
5.3.5 Remove the protective cap from the output end of the light guide. Insert the light
guide into the input portion of the collimating adapter until it is flush with the
adjustable insert. Secure the light guide to the collimating adapter by tightening
the thumbscrew. Do not over tighten.
Note: The light guide has a minimum bend radius of 1.6 inches (40.0mm).
Bending or coiling the light guide tighter than this radius will result in
permanent damage to the light guide.
X-Cite® 200DC User Guide
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15
Warning!
Turning power to the on position without the light guide properly installed in the
unit and in the microscope can increase your risk of exposure to UV light.
Figure 7 Collimating Adapter
X-Cite® 200DC User Guide
035-00446R rev. 4
16
Warning! Turning power on to the on position without the light guide properly
installed in the unit and in the microscope can increase your risk
of exposure to UV light.
Lamp Warm-Up:
The ARC lamp has 3 distinct phases of operation;
1. Ignition.
2. Warm-up. Excelitas Technologies recommends 20 minutes of
proper warm-up to ensure a stable optical output.
3. Stable Operation.
It is recommended that phase 1 and 2 are not interrupted. This can result in
shortened lamp life. The lamp must be allowed to warm-up uninterrupted.
6 Operation
6.1 Powering Up/Powering Down
6.1.1 Ensure that the lamp and light guide have been properly installed and that the
lamp housing panel is securely fastened.
6.1.2 Plug the X-Cite® 200DC unit into a properly grounded AC outlet.
6.1.3 Turn on the main POWER switch “I”, located on the front panel and check the fan
for airflow.
6.1.4 As soon as the LED display turns on, it will display the version of software
currently programmed in the unit (i.e. R – X). Once the software version level has
been indicated, the display will reset after several seconds to the next display
mode.
6.1.5 The lamp will automatically turn on within 45 seconds and the LAMP indicator will
illuminate (green). The display will flash during the warm up period for
approximately 4 minutes. The display will stop flashing when the warm up period
has completed. Wait until the warm up period has completed before using the
unit to illuminate a specimen. Excelitas Technologies recommends 20
minutes to ensure a stable output
Note: Unit should not be turned off unless the lamp has been on for a minimum
of 20 minutes.
X-Cite® 200DC User Guide
035-00446R rev. 4
17
Button
Condition
Description
I/O
Press on press off
Turns unit on “I” Turns unit off “0”
SHUTTER
Press and release
Opens/closes internal shutter
LED Indicator
Status
Description
LIGHT GUIDE
On – green
The light guide is fully inserted
On – red
The light guide is not fully inserted
LAMP
On - green
The lamp is on
Off – no illumination
The lamp is off
OPEN
On - green
The shutter is open
Off – no illumination
The shutter is closed
6.1.6 To power down the unit after use, set the main power switch located on the front
panel to “0”.
Note: If the lamp is turned off, and an attempt is made to turn it back on before it
has fully cooled, the “Cool” message will appear on the display and the
lamp indicator will be un-lit. The lamp will automatically re-strike when the
lamp has cooled, the lamp indicator will illuminate (green).
6.2 Illuminating a Specimen
6.2.1 When the X-Cite® 200DC is first powered on, the internal shutter will be in the
closed position as a safety precaution. If the shutter is not closed error code SFI
will appear on the LED display.
6.2.2 To illuminate a specimen, press and release the SHUTTER button to open the
shutter (OPEN LED will turn on).
6.2.3 Use “Iris Adjustment Dial” for intensity adjustment to desired level.
6.2.4 Press the SHUTTER button to close the shutter. The OPEN LED will turn off.
6.3 Front Panel Button Functions
6.4 LED Indicators
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18
Display
Format
Definition
Description
XXXX.
Lamp Hours
Displays the “hours of use” accumulated by the
lamp
Flashing:
“XXXX”
Warming Up
The lamp is warming up. Warm up time is
approximately 4 minutes
6.5 Display Format Reference
6.5.1 The formatting used on the display board is used to identify the unit of
measurement or phase of operation.
6.6 Adjusting Light Output
Figure 8 Iris Adjustment Dial Layout
6.6.1 To adjust light intensity, use the Iris Adjustment Dial and set to desired level by
turning. Clockwise to increase light intensity and counter-clockwise to decrease
light intensity.
6.6.2 There are no detent stops, motion is smooth starting from 0% and ending at
100%. 0, 12.5, 25, 50, 100% are marked on the fascia around the dial for
reference (ref. Figure 8)
6.6.3 Note that as the lamp ages, absolute power at a given setting will decrease.
Adjust the intensity as necessary. If monitoring absolute value of lamp output is
desired, refer to section 6.8 or contact Excelitas Technologies about purchasing
an X-Cite® Optical Power Measurement System (OPMS).
X-Cite® 200DC User Guide
035-00446R rev. 4
19
6.7 Viewing Accumulated Lamp Hours
6.7.1 The X-Cite® 200DC system automatically accumulates the number of hours for
which the lamp has been on by saving it to the Intelli-Chip on the lamp, and
shows this information on the LED display. The lamp hours are shown when the
unit is powered on and after lamp has warmed up.
6.8 Power Measurement (Optional)
6.8.1 If your X-Cite® 200DC was purchased with the X-Cite® Optical Power
Measurement System accessory, power output can be measured at the end of
the light guide using the XR2100 or at the specimen plane using the XP750.
Note: To learn more about the OPMS or to purchase the OPMS accessory
contact ExcelitasTechnologies.
XR2100 Optical Power Meter with Light Guide Input;
1. Install the supplied light guide adaptor corresponding to the size of your light
guide into the Optical Input Port of the XR2100
2. Ensure the X-Cite® 200DC shutter is closed and set Iris to the desired intensity
level.
3. Remove light guide from microscope adapter and insert the light guide end into
the light guide adaptor and fasten in place using the supplied thumb screw.
4. Open shutter and turn the XR2100 to ON.
5. Use Relative/Absolute button on the XR2100 to adjust display mode of
measurement.
XP750 Objective Plane Power Meter Sensor (with upright microscopes);
1. Plug the XP750 multi-pin connector into the XR2100, turn the XR2100 to ON.
2. Place the XP750 onto the stage with the detection window facing up, and
approximately centered beneath the microscope objective lens.
3. Secure the XP750 in place using the microscope’s stage clip and the supplied
suction-cup ensuring the XP750 is seated firmly and sits flush on the stage.
4. Turn on the epi-fluorescence light source and open the microscope shutter,
ensure that the filter cube in the beam path is directing visible light to the
microscope stage
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20
5. To align the detection window with the visible light from the microscope objective,
use the microscope stage x-y controls, ensuring the light is centered within the
window.
6. Engage the appropriate objective, filter cube, intensity and other microscope
settings and press the ‘(nm)’ button on the XR2100 to see the current
wavelength setting.
XP750 Objective Plane Power Meter Sensor (with inverted microscopes);
1. Plug the XP750 multi-pin connector into the XR2100 turn the XR2100 to ON.
2. Place the XP750 onto the stage with the detection window facing down, and
approximately centered beneath the microscope objective lens. The + icon
(etched inside a circle) should be facing up which marks the center of the
detection window.
3. Secure the XP750 in place using the microscope’s stage clip and the supplied
suction-cup ensuring the XP750 is seated firmly and turn on the transmission
light source and using the microscope stage x-y controls, center the light on the +
icon.
4. Engage the appropriate objective, filter cube, intensity and other microscope
settings and press the ‘(nm)’ button on the XR2100 to see the current
wavelength setting.
Note: To learn more about the OPMS or to purchase the OPMS accessory
contact Excelitas Technologies.
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21
Alarm Condition
Alarm
Type
Other Actions/
Corrections
Light guide is not inserted
properly and/or removed
while shutter is open or an
attempt is made to open the
shutter
LIGHT GUIDE
LED turns red, “3
beep” audible
alarm
Critical
Safety
Shutter shall
automatically close and
be disabled from opening
until light guide is inserted
Lamp failure to strike
Display “BULB”
Function
Lamp too hot to strike
Display “CooL”
Function
Automatically attempts to
re-strike lamp when cool
Shutter failure
“3 beep” audible
alarm
display “SF1”
Critical
Safety
If failure to close—lamp
turns off
Lamp overheated
“3 beep” audible
alarm.
Display “E1”
Critical
Safety
Lamp turns off
Lamp self-extinguish or nonpassive failure
“3 beep” audible
alarm.
Display “E6”
Critical
Safety
Fan/blower shall shut off
6.9 Warnings and Alarms
6.9.1 The X-Cite® 200DC is designed to detect certain conditions that may affect safe
or proper functioning. These conditions are categorized as one of two “alarm types” according to the danger they pose to the operator, system or experiment:
1. Critical Safety – continuing may cause harm to the operator or equipment,
alarm is an audible 3 beep “Alarm Condition”.
2. Function – operation may continue, but depending on specific alarm
condition, functionality of unit may be limited or impossible (e.g. lamp too hot
to strike)
6.10 Clearing Alarms
6.10.1 The X-Cite® 200DC provides an audible alarm (3 beeps only) to alert the operator
X-Cite® 200DC User Guide
035-00446R rev. 4
of various error conditions. After the 3 beeps have counted off, the alarm will
automatically shut off.
22
Interval
Description
Time
(milliseconds)
t1
Delay time, trigger to start of opening
4
t2
Start of opening to 100% open
2
t3
Minimum recommended exposure time (trigger
open to trigger close)
40
t4
Delay time, trigger to start of close
4
t5
Start of close to 0% open
2
TRIGGER
RESPONSE
t1 t2
t4 t5
SYNC.O/P
80%
t3
SHUTTER
RESPONSE
SYNC
OUTPUT
7 Shutter Control
7.1 Shutter Control via Foot pedal
7.1.1 For hands-free operation, the X-Cite® 200DC is equipped with a foot pedal. The
foot pedal is an alternative to the SHUTTER button and can only be used to open
and close the internal shutter. To use the foot pedal:
1. Plug the foot pedal into the 3.5mm audio style port labelled FP on the back
on the X-Cite® 200DC.
2. Place the foot pedal on the floor or another conveniently located flat surface.
3. Power-up the X-Cite® 200DC as normal, and wait for the lamp to warm up.
4. Press down on the foot pedal until it clicks to open or close the shutter.
7.2 Shutter control via TTL
7.2.1 For high-speed shutter activation, TTL control can also be used. The plot below
shows typical timing values for the X-Cite® 200DC internal shutter when
controlled by TTL. Note that these values should be used as a guide only, and
that actual values will vary slightly from unit to unit.
Note: Using values of t3<40ms may result in shutter bounce-back and a brief
X-Cite® 200DC User Guide
035-00446R rev. 4
unintentional exposure period.
23
TTL
Shutter
Sync
high
open
low
low
closed
high
TTL Signal and Shutter Status
TTL INPUT (BNC) SYNC OUT (BNC)
Maximum low-level +0.8VDC Maximum low-level +0.8VDC
Minimum high-level +2.0VDC Minimum high-level +4.5VDC
Maximum high-level +5.5VDC Maximum output current: 500uA
Typical input current: 800uA
24
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Code
Error Description
Recommended Action
E1
Lamp temperature is too high
Check ventilation filters and outlets to ensure
that no blockage exists. Restart unit and if
problem persists replace lamp. If problem
continues contact Tech Support. See also
section 8.7
E4
Internal hardware failure
Restart unit, if problem persists contact Tech
Support
E5
Failure to communicate with
Intelli-Lamp
Check Intelli-Lamp connection and restart unit.
If problem persists try another lamp, if problem
still persist contact Tech Support.
E6
Lamp self-extinguished
Let system cool and restart lamp. If it goes out
again, replace lamp. If new lamp continues to
self-extinguish, contact Tech Support
E7
Internal system error
Restart unit, if problem persists contact Tech
Support
bulb
Lamp Error
Lamp installed incorrectly /Lamp did not strike.
cool
Lamp is too hot to strike
The lamp will automatically strike when it has
cooled to the optimum striking temperature
Alternating:
old / bulb
Old Lamp
The lamp has accumulated over 2500 hours.
Lamp may be near end of life.
Alternating:
end / bulb
New Lamp Required
The lamp has reached end of life. The lamp
will not strike.
SFI
Shutter Failure
The shutter has failed to return to home
position. Unit should be restarted. If the error
repeats, contact Tech Support.
8 Troubleshooting
Service to be completed by qualified repair personnel only!
8.1 Error Codes
If the X-Cite® 200DC internal monitoring systems identify a problem, an error code will
be generated. Definitions and recommended actions are in the following table.
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8.2 Power Up Failure
If the unit fails to POWER up or function properly, use the following checklist to
eliminate the most common causes of problems. Check that:
1. The AC POWER cord is securely plugged into a functional AC wall plug.
2. The AC POWER cord is securely plugged into the AC inlet on the rear of the unit.
3. The main AC POWER switch is in the ON position.
4. Check that the ventilation openings on both the bottom and rear of the unit are not
blocked.
If the unit still does not power-up:
5. Check both main power fuses by first disconnecting the power cord. Then carefully
remove the fuse drawer assembly below the AC inlet on the rear of the unit. If the fuse(s)
is/ are open, replace with the same type (5A, 250 V, Fast acting).
8.3 Lamp Strike Failure
If the LED display lights and the fan starts, but the lamp will not turn on, check for
the following:
1. The LED display indicates the “bulb” message and the system begins to beep. This
indicates that no lamp is detected. Check if the lamp has been installed correctly. Refer
to section 5.1– Installing the Lamp Module.
2. The LED display indicates the “bulb” message after approximately 45 seconds and
the system will beep 3 times as an audible warning. This indicates that the lamp has
failed to strike. It may be a result of the lamp reaching end of life, or that the lamp
housing panel is not secured properly in place. Press the MODE button to clear the
audible alarm. Turn power off to the unit. Check that the lamp housing panel is secured
properly. Wait a few minutes and turn the power on to the unit. If it still does not strike,
replace the lamp.
3. The LED display indicates the “Cool” message. This indicates the lamp is too hot to
strike. The lamp will automatically strike when it has cooled.
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8.4 Low Light Intensity
If the light intensity is too low, check that:
1. The percent iris opening is set high enough, if not turn Iris Adjustment Dial to a higher
setting.
2. There are no foreign substances on the emitting end of the light guide.
3. There are no bends, kinks, or other physical damage to the guide. Replace the light
guide if there is any physical damage.
4. The lamp has been installed correctly. See section 5.1– Installing the Lamp Module.
5. Check the microscope settings, filter cubes, ND filters, manual stops, polarisers, and
position of camera port beam splitters for anything that may be blocking the light path
between the X-Cite® and eyepieces/camera.
It may be necessary to replace the lamp or to replace the light guide. Contact your
Excelitas Technologies sales representative for information on purchasing a new lamp or
light guide.
8.5 Shutter Failure
If the shutter does not open, check that:
1. The light guide is fully inserted; the LED above the light guide port will be illuminated
green. The lamp is warmed-up; the display is not flashing. The iris setting is not at 0%.
2. If the LED displays “SF1”, indicating a shutter failure. POWER down the unit, wait a
few minutes and turn POWER on to the unit. If the unit displays a shutter failure again,
contact your local ExcelitasTechnologies Service Center to have your unit serviced.
8.6 LED Display Failure
If the LED display does not light:
1. If the fan is functional, POWER down the unit, wait approximately 20 seconds then
POWER it up again.
2. If the problem persists, contact your local Excelitas Technologies Service Center.
8.7 Cooling Fan Failure
If one or more fans do not work:
1. If the LED display is functional, POWER down the unit, wait approximately 20
seconds then POWER it up again.
2. If the problem persists, contact your local Excelitas Technologies Service Center.
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9 Care and Maintenance
9.1 General
This section contains installation, maintenance and troubleshooting tips to help optimize
the lifetime and performance of X-Cite® 200DC units and components. This information
is intended to supplement the detailed installation and operating instructions found in the
X-Cite® User Manuals. Topics covered include liquid light guides, lamps, and air vents –
the components most likely to affect performance over time and require maintenance or
replacement. Note that the lifetime information on components is for typical or average
conditions. Depending on the imaging application, more frequent replacement may be
required—for example, if a fluorophore’s signal is low to begin with, or is excited by a low
power portion of the X-Cite® 200DC lamp’s output spectrum.
9.1.1 Operate the unit in a well ventilated area with at least six inches clearance at the
rear of the unit for proper air flow. Do not place any objects below the unit,
between the feet as this will restrict airflow through the bottom of the front face
plate.
9.1.2 For safe operation, use only a grounded outlet.
9.1.3 Avoid physical shocks or jarring to the unit especially while the unit is operating.
Such sudden movements reduce the lamp module life.
9.1.4 The lamp module must be operated for a minimum of 20 minutes each time it is
turned on to prevent damaging the lamp. Increasing the time between turning
the lamp module on and off will maximize lamp life.
9.1.5 Replace the air filter, found under the front face plate, frequently to ensure
unrestricted air flow. It is recommended as a minimum that the air filter be
replaced every time the lamp module is replaced.
Note: restricted airflow can cause the lamp temperature to increase above
optimum temperature, significantly reducing lamp life.
9.1.6 When necessary, clean the light emitting end of the light guide using an optical
cleaning solution.
9.1.7 Cleaning of unit is not required, however if cleaning is desired, disconnect the AC
power cord from the unit and use only a water and simple detergent solution.
Ensure that cleaning solution does not come in contact with any optical, moving
mechanical or electrical parts.
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9.2 Liquid Light Guides
Tips to Prevent Premature Degradation of Light Guides
9.2.1 Liquid light guides have a typical useful life of 2-3 years/4000 operational hours
in the X-Cite® 200DC when installed and handled properly. The formation of
bubbles is one of the most common reasons for a light guide to degrade
prematurely and result in a sudden reduction in illumination intensity. Bubbles
form without warning, usually due to overheating and/or mechanical stress to the
light guide. Below are some simple tips to avoid overheating and stressing the
light guide.
9.2.2 The light guide has a minimum bend radius of 1.6 inches (40.0mm) however, a
bend radius of at least 75mm is recommended for a light guide while “in use”.
Sharper bends can cause heat to build up and cause problems longer term.
Bending or coiling the light guide tighter than this radius will result in permanent
damage to the light guide. Minimum bend radius to prevent immediate damage to
the LLG is 40mm,.
9.2.3 Make sure the light guide is cooled properly during use, and prevent overheating:
a. Always fully insert the light guide in to the X-Cite® 200DC; this ensures
contact with a heat sink to conduct heat away from the light guide.
b. Never obstruct the air vents on the X-Cite® 200DC. Vents are located at the
rear and underside of the unit.
c. Do not remove the rubber feet on the X-Cite® 200DC or otherwise
reduce/block the space between the bottom of the unit and bench top. This
may compromise airflow through the unit.
d. Ensure that the air being used to ventilate the X-Cite® 200DC is
approximately “room temperature” (e.g. do not place the X-Cite® unit on top
of another heat-producing instrument).
e. If a heated environmental chamber is being used for live cell imaging, make
sure that the X-Cite® 200DC and the light guide are located outside of the
chamber.
9.2.4 Do not expose the light guide to extreme temperatures (above 35°C, below -5°C)
for extended periods of time during use, transport or storage; this may cause
degradation of the seals and allow air bubbles to form in the liquid.
9.2.5 Never kink, bend, crush, or stretch the light guide; this type of mechanical stress
may cause bubbles to form in the liquid and/or damage to the outer sheath
a. Always allow adequate clearance between the light guide port of the X-Cite®
200DC and other objects to prevent excessive bending.
b. Place the X-Cite® 200DC close enough to the microscope so that there is
some slack in the light guide and no sharp bends.
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9.2.6 Never leave an endcap on the output end of the light guide when the other end is
connected to the X-Cite® 200DC; if the unit is turned on in this condition, the cap
will overheat, melt and/or permanently discolour the quartz end of the light guide.
9.2.7 While the X-Cite® 200DC is on but not in actual use (i.e. during sample
preparation, or between time lapse time points) close the iris/shutter on the XCite®; this reduces unnecessary UV photon load on the liquid light guide. (Using
only the shutter/stop in the microscope itself protects the specimen, but energy is
still passing through the light guide.)
Proper handling and installation of liquid light guides
9.2.8 Liquid light guides have a typical useful life of 4000 hours of operation when
handled properly and installed in a well maintained X-Cite® 200DC. The
formation of bubbles is one of the most common reasons for a light guide to
degrade prematurely and result in a sudden reduction in illumination intensity.
Bubbles can form without warning, and if they occur within the first 1500 to 2000
hours of use, is it typically due to overheating and/or mechanical stress to the
light guide. Below are some simple tips to avoid overheating and stressing the
light guide;
9.2.9 Always fully insert the light guide in to the X-Cite® 200DC (refer to section 5.2);
this ensures contact with a heat sink to conduct heat away from the light guide.
9.2.10 Always place the X-Cite® 200DC close enough to the microscope so that there is
some slack in the light guide and no sharp bends.
9.2.11 Never leave an endcap on the output end of the light guide when the other end is
connected to the X-Cite® 200DC; if the unit is turned on in this condition, the cap
will overheat, melt and/or permanently discolour the quartz end of the light guide.
9.2.12 Do not expose the light guide to extreme temperatures (above 35°C, below -5°C)
for extended periods of time during use, transport or storage; this may cause
degradation of the seals and allow air bubbles to form in the liquid.
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100% 11.3% 5.2%
What does a bubble look like?
9.2.13 Depending on the size and location in the light guide, a bubble may or may not
be obvious. To check for bubbles:
9.2.14 Disconnect the LLG from the X-Cite® 200DC and microscope adapter.
9.2.15 Hold one end towards a bright window or overhead room light - DO NOT use an
X-Cite® 200DC or any other focused light source for this test!
9.2.16 Look at the quartz at the other end of the LLG
a. Bubble-free: quartz end will appear as a bright, solid circle; you may also be
able to see a thin circular outline at the quartz/liquid interface.
b. Bubbles at/near the quartz end: appear as dark spots, as small as 0.5mm in
diameter or even as larger more defined spheres.
c. Bubbles in the middle of the light guide: may not be well-defined spots, but
will appear as dark shadows.
d. In extreme cases, where the bubble is blocking the entire diameter of the light
guide, no light will come through, even when pointing the distal end at a light
source.
Appearance and % output of light guides
with bubbles relative to an LLG without bubbles (100%).
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Can a bubbled light guide recover?
9.2.17 Yes, light guides with small bubbles can sometimes recover. Disconnect the light
guide from the X-Cite® 200DC, and leave the light guide undisturbed on a shelf
for 1-2 weeks. For this to be effective, it is important to catch the bubble when it
is small.
When should light guides be replaced?
9.2.18 It is usually time to replace a light guide when:
1. Illumination is low and replacing the lamp does not improve brightness
2. Dark or uneven areas become visible in the field of view (a bubble is blocking
part of the light)
3. A section of the light guide becomes noticeably warmer than the rest of the guide
(a bubble is blocking transmission of light, forcing the light guide to absorb the
energy)
4. It is 2-3 years old OR has been in use for 4000-6000 hours (2-3 lamp changes)
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Lamp Output Spectrum X-Cite® 200DC
9.3 Lamps
Spectrum and lifetime
9.3.1 The lamp output and typical output levels over lifetime are shown below.
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X-Cite® 200DC Lamp: Typical Degradation
9.3.2 It is normal for the output to decline over the first several hundred hours of use
and then stabilize at a level ~70% of the initial output for the remainder of the
lamp life. If the lower power regions of the output spectra are being used, it may
be desirable to replace lamps more frequently to maximize signal levels.
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General tips to help maximize lamp lifetime:
1. Each time the lamp is ignited, the stress on the lamp effectively decreases lamp life
by 5 hours, therefore it is recommended to avoid shutting down the unit during lunch
or short breaks or between users, if being used in a multi-user lab
2. After lamp ignition, avoid turning the lamp off until it has run for a minimum of 20
minutes
3. Avoid attempting to strike a hot lamp (note: the X-Cite® 200DC Intelli-Lamp will
prevent this)
4. Avoid unnecessary movement and jarring of the lamp, especially when it is in
operation or hot.
5. When changing a lamp:
a. Handle the lamp only by the ceramic areas
b. Never touch the glass envelope of the bulb (inner stem), the inner surface, or
the outer surface of the reflector. If touched, carefully clean the envelope with
alcohol. Skin oils can etch the glass and cause premature bulb failure
c. Wear cotton gloves or powder free latex/nitrile gloves when handling any
lamp
6. Ensure that air filters are kept clear of dust and debris – even a partially blocked filter
can reduce lamp lifetime to approximately 25% of what is normally expected.
9.4 Replacing the Lamp Module
9.4.1 The X-Cite® 200DC is designed to operate only with the X-Cite® 200DC lamp
module supplied by an authorized Excelitas Technologies distributor. When the
lamp requires replacing, use only:
Excelitas Technologies Part # Description
012-66000R X-Cite® 200DC/exacte
replacement lamp module
Refer to section 5.1 for lamp module replacement instructions
Refer to section 8 for messages regarding troubleshooting and lamp module
replacement.
Refer to section 3.1 Glossary of Symbols, for lamp warning information.
Refer to section 6.1 Powering Up/Powering Down, powering up/down
instructions.
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9.5 Air Vents & Filters
9.5.1 X-Cite® 200DC has several air vents that are an integral part of the cooling
system and overall lamp performance. Proper cooling ensures that lamps
operate at optimal temperature and pressure for output power, spectrum, lamp
life, light guide life and safety.
Ensuring adequate cooling
9.5.2 Never obstruct the air vents on the X-Cite® 200DC. Vents are located at the rear
and underside of the unit.
9.5.3 Always leave clearance for air flow between the X-Cite® 200DC and walls or
other equipment. Do not remove the rubber feet on the X-Cite® 200DC unit or
otherwise reduce/block the space between the bottom of the unit and bench top.
This may compromise airflow through the unit.
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9.5.4 Ensure that the air being used to ventilate the X-Cite® 200DC is approximately
“room temperature” (e.g. do not place the X-Cite® 200DC on top of another heat-
producing instrument).
9.5.5 If a heated environmental chamber is being used for live cell imaging, make sure
that the X-Cite® 200DC and the light guide are located outside of the chamber.
9.5.6 Periodically check the air filter at the air intake vent for debris. Replace or clean
as necessary
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How to Check/Clean Metal Vents
9.5.7 When unit is OFF and lamp is COOL, unplug power from X-Cite® 200DC and turn
unit upside down. Visually inspect the metal gratings for lint, dust and other
debris.
9.5.8 Debris can be removed from the gratings using a small vacuum cleaner with a
crevice tool, or blown out with a can of compressed air. If using compressed air,
direct the air flow ACROSS the vents, not perpendicularly (i.e. into the X-Cite®
200DC).
9.6 Replacing the Air Filter
9.6.1 The external air filter is located under the front face plate of the X-Cite® 200DC.
9.6.2 Turn off the main POWER switch and remove the AC POWER cord from the unit.
9.6.3 Gently slide and pull out the filter.
9.6.4 Push in the replacement filter so that it sits flat in place
What happens if filters and vents are not clean?
9.6.5 A complete filter blockage or airflow obstruction generally results in an automatic
lamp shutoff within 10-15 minutes of the unit being powered on due to lack of
adequate cooling.
9.6.6 A partially clogged filter may allow enough airflow for continued operation, but not
for optimal cooling. In this case, the result is usually dramatically reduced lamp
life, e.g. 400 hours instead of 2500+ hours.
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9.7 Replacing the External Fuses
9.7.1 The external (main) fuses are located in the fuse drawer which is located in the
AC inlet module on the rear panel.
9.7.2 Turn off the main POWER switch and remove the AC POWER cord from the unit.
9.7.3 Gently pull out the drawer with the aid of a flat-head screwdriver.
9.7.4 Carefully lever one end of each fuse up from its retaining clip with a small flathead screwdriver and lift it out.
9.7.5 Replace the blown fuse(s) only with the same type and rating (F5A, 250V). The
rear compartment must contain two active fuses.
9.7.6 Close the fuse drawer.
9.7.7 Reconnect the AC POWER cord.
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10 Technical Specifications
10.1 Electrical
Power Supply: Power Factor Corrected, Universal Input
Input Voltage: 100 - 240VAC, 50/60Hz
Current: 3.5A max at 120VAC
2.0A max at 240VAC
Input Surge: 50A max. (cold start)
Protection: Power supply:
Note: X-Cite® 200DC unit dimensions only, does not include clearance at front of
unit for liquid light guide.
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11 Regulatory
11.1 Product Safety and Electromagnetic Compatibility:
The X-Cite 200DC has been tested and found to comply with product safety and
electromagnetic compatibility requirements. For a complete list of tests and for
certification details, please contact your X-Cite representative or visit
Council Directive 2014/35/EU Low Voltage Directive
Council Directive 2014/30/EU EMC Directive
Council Directive 2012/19/EU WEEE Directive
Council Directive 2011/65/EU RoHS
FCC Class A Digital Device or Peripheral - Information to User
NOTE
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with the instruction
manual, may cause harmful interference to radio communications. Operation of this
equipment in a residential area is likely to cause harmful interference in which case the
user will be required to correct the interference at his own expense.
WARNING
Changes or modifications not expressly approved by Excelitas Technologies could void
the user’s authority to operate the equipment.
WEEE Directive (2012/19/EU)
The symbol above indicates that this product should not be disposed of along with
municipal waste, that the product should be collected separately, and that a separate
collection system exists for all products that contain this symbol within member states of
the European Union.
The equipment that you bought has required the extraction and use of natural
resources for its production. It may contain hazardous substances that could
impact health and the environment.
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In order to avoid the dissemination of those substances in our environment and
to diminish the pressure on the natural resources, we encourage you to use the
appropriate take-back systems. Those systems will reuse or recycle most of the
materials of your end life equipment in a sound way.
The crossed-out wheeled bin symbol indicated above invites you to use those
systems.
If you need more information on the collection, reuse and recycling systems,
please contact your local or regional waste administration.
11.2 China RoHS
The symbol above indicates that this product is in compliance with China RoHS
requirements.
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12 Warranty
Excelitas Technologies warrants the original purchaser for a period of one (1) full year,
calculated from the date of purchase, that the equipment sold is free from defects in
material and workmanship.
In the event of a claim under this guarantee, the equipment is to be sent postage and
carriage paid, including a description of the fault, to the Excelitas Technologies Service
Center. Returned equipment will not be received without a Return Authorization (RA)
Number, issued by the appropriate Service Center.
In the case of damage caused by wear and tear, careless handling, neglect, by the use
of force or in the case of interventions and repairs not carried out by an Excelitas
Technologies Service Center, the guarantee ceases to be valid. This guarantee may not
form the basis for any claims for damages, in particular not for compensation of
consequential damages.
The warranty is not transferable. No warranty is extended to perishable items, such as
fuses, air filters and light guides.
Any claims for units received with defects in material or workmanship must be reported
to an authorized Excelitas Technologies Service Center within 30 days from the original
date of receipt.
Replacement Bulb Warranty
If the X-Cite® 200DC bulb fails to strike during the warranty period of 2000 hours, the
bulb will be replaced under warranty, or a credit will be applied to the purchaser’s
account. In the event of a claim under this guarantee, the lamp is to be sent postage
and carriage paid, including a description of the fault, to the Excelitas Technologies
Service Center. Returned equipment will not be received without a Return Authorization
(RA) Number, issued by the appropriate Service Center. Lamps must be purchased
from an authorized Excelitas Technologies Representative or Distributor to be eligible for
the warranty replacement. This warranty is non-transferable.
In the case of damage caused by careless handling, neglect, by the use of force or in the
case of interventions and repairs not carried out by an Excelitas Technologies Service
Center to the X-Cite® 200DC system, the guarantee ceases to be valid.
Returning equipment to ExcelitasTechnologies
1. Please make a note of the problem encountered, the steps followed to isolate the
problem and the result of any trouble shooting steps taken.
2. Contact the nearest Excelitas Technologies Service Center to obtain a Return
Authorization Number. For your convenience, RA numbers can also be requested online at: http://www.excelitas.com/Pages/Support/Service-Request-Form.aspx
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Part Number
Description
010-00283R
X-Cite® 200DC Illumination System
012-66000R
X-Cite® 200DC/Exacte Lamp Module
805-00038
X-Cite® Liquid Light Guide 3mm x 1.5m
805-00040
X-Cite® Liquid Light Guide 3mm x 3.0m
622-00012R
Replacement Air Filter
850-00023R
Hex Key, 3mm (lamp access cover)
035-00447R
X-Cite® 200DC Quick Start English
010-00245R
Optical Power Measurement System includes;
- X-Cite® XR2100 Optical Power Meter (010-00242R)
- X-Cite® XP750 Objective Plane Power Sensor (010-00241R)
3. Follow shipping instructions provided by the service technician. The unit should be
returned in its original packaging if possible. Please do not ship the unit with the lamp
installed.
Replacement parts and accessories can be purchased directly from Excelitas
Technologies. For ordering and pricing information contact the inside sales department
at: