EUTECH INSTRUMENTS DO 6 - REV 2, DO 6 Instruction Manual
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Instruction Manual
DO 6
Economy Hand-held Dissolved Oxygen Meter
68X243622
Rev 2 Apr 08
Part of Thermo Fisher Scientific
Preface
This manual serves to explain the use of the DO 6 Dissolved Oxygen Handheld.
This manual functions in two ways: first as a step by step guide to help you
operate the meter; second, as a handy reference guide.
This manual is written to cover as many anticipated applications of the DO6
palm-top meter as possible. If there are doubts in the use of the Dissolved
Oxygen Handheld, please do not hesitate to contact the nearest Eutech
Instruments / Oakton Instruments authorized Distributor.
Eutech Instruments/Oakton Instruments will not accept any responsibility for
damage or malfunction to the meter caused by improper use of the instrument.
The information presented in this manual is subject to change without notice as
improvements are made, and does not represent a commitment on the part of
Eutech Instruments / Oakton Instruments.
Copyright © 2004
Eutech Instruments Pte Ltd
Oakton Instruments
All rights reserved.
TABLE OF CONTENTS
1 INTRODUCTION ..........................................................................1
2 DISPLAY & KEYPAD FUNCTIONS.............................................2
2.1 DISPLAY .................................................................................2
2.2 K
EYPAD ..................................................................................3
3 PREPARATION............................................................................4
3.1 INSERTING & REMOVING RUBBER BOOT ................................4
3.2 INSERTING THE BATTERIES .....................................................5
3.3 BATTERY REPLACEMENT........................................................6
3.4 D
ISSOLVED OXYGEN PROBE INFORMATION............................7
3.5 CONNECTING THE PROBE TO METER ......................................9
3.6 SWITCHING THE METER ON ..................................................10
3.7 M
EASUREMENT MODE SELECTION:- PERCENTAGE
SATURATION (%), CONCENTRATION (MG/L)(PPM) AND
TEMPERATURE(T).................................................................11
4 CALIBRATION...........................................................................12
4.1 IMPORTANT INFORMATION ON METER CALIBRATION ...........12
4.2 P
REPARING THE METER FOR CALIBRATION ..........................13
4.3 TEMPERATURE CALIBRATION ...............................................15
4.4 DISSOLVED OXYGEN CALIBRATION IN % SATURATION........17
4.4.1 To calibrate 100% Saturation:................................................17
4.4.2 To calibrate 0% Saturation.....................................................19
4.5 DISSOLVED OXYGEN CALIBRATION IN MG/L OR PPM
CONCENTRATION MODE
........................................................20
4.5.1 To calibrate in mg/L (ppm) Concentration mode:...................20
5 MEASUREMENT........................................................................22
5.1 WITH AUTOMATIC TEMPERATURE COMPENSATION (ATC) ..22
5.2 MANUAL TEMPERATURE COMPENSATION ............................23
5.3 T
AKING MEASUREMENTS .....................................................25
5.3.1 To take measurements: .........................................................25
5.4 T
AKING PRESSURE/SALINITY COMPENSATED DO
MEASUREMENTS...................................................................26
5.4.1 Pressure Setting Adjustment.................................................. 26
5.4.2 Salinity Setting Adjustment ....................................................29
6 HOLD FUNCTION......................................................................30
7 ADVANCED SETUP FUNCTIONS.............................................31
7.1 ADVANCED SETUP OVERVIEW..............................................31
7.1.1 To enter the % Saturation or Temperature Set Up menu: ..... 31
7.1.2 To enter the mg/L (ppm) Concentration Set Up menu:..........31
7.2 (COF.1) CONFIGURATION MENU..........................................36
7.2.1 Selection of Automatic Temperature Compensation.............. 36
7.2.2 Selection of mg/L or ppm mode .............................................38
7.3 (CAL.2) CALIBRATION DATA...............................................39
7.3.1 Viewing the % Saturation calibration data.............................. 39
7.3.2 Viewing the mg/L (ppm) Concentration calibration data ........39
7.4 (ELE.3) ELECTRODE PROPERTIES.........................................40
7.4.1 Viewing the electrode Slope Factor .......................................40
7.4.2 Viewing the % Saturation Offset (Only available in %
Saturation or Temperature Set Up menu):............................................... 40
7.4.3 Viewing the 100% Saturation mV value.................................41
7.4.4 Viewing the 0% Saturation mV value.....................................41
7.5 (ATO.4) AUTOMATIC OFF ....................................................43
7.6 (
RST.5) RESET TO FACTORY DEFAULT..................................44
7.6.1 Calibration Reset....................................................................44
7.6.2 User Reset .............................................................................46
7.7 (OFS.6) % SATURATION OFFSET ADJUSTMENT....................47
7.8 (DPR.7) DISSOLVED OXYGEN PARAMETERS.........................49
7.8.1 Pressure Setting Adjustment.................................................. 49
7.8.2 Salinity Setting Adjustment ....................................................51
8 PROBE CARE AND MAINTENANCE........................................52
8.1 PROBE CARE.........................................................................53
8.2 PRE-MEMBRANED CAPS REPLACEMENT...............................53
8.3 T
O REPLACE THE PROBE PRE-MEMBRANED CAP ....................54
8.4 ELECTROLYTE SOLUTION .....................................................55
9 TROUBLE-SHOOTING GUIDE..................................................56
10 ERROR MESSAGES..................................................................57
11 FACTORY DEFAULT SETTINGS..............................................58
12 SPECIFICATIONS......................................................................60
13 ACCESSORIES..........................................................................62
14 ADDITIONAL INFORMATION....................................................63
14.1 DISSOLVED OXYGEN ............................................................63
14.1.1 General Information ...............................................................63
14.1.2 Measurement Units................................................................65
14.1.3 What Is Being Measured?......................................................65
14.1.4 Air Calibration.........................................................................66
14.1.5 Applications............................................................................66
15 WARRANTY...............................................................................68
16 RETURN OF ITEMS...................................................................69
Instruction Manual DO 6
1
1 INTRODUCTION
Thank you for purchasing the DO 6 Dissolved Oxygen Handheld. This economy
microprocessor-based handheld has a large custom LCD (Liquid Crystal
Display) for clear and easy reading.
The DO 6 offers the measurement of dissolved oxygen in the percentage
saturation mode and in the concentration mode. Temperature measurement is
also available in degrees Celsius. The meter ensures accurate measurement of
the Dissolved Oxygen values through its temperature, barometric pressure and
salinity compensation features.
Your meter includes a dissolved oxygen probe, refill solution, a rubber boot, 4
alkaline “AAA” batteries, and instruction manual.
Please read this manual thoroughly before operating your meter.
To order other accessories and standard solutions, please refer to the Section
on Accessories for more information.
Instruction Manual DO 6
2
2 DISPLAY & KEYPAD FUNCTIONS
2.1 Display
The meter has a large custom LCD that consists of 14-segment 4 digit display
and operation annunciators for Percentage Saturation measurement mode (%),
Concentration measurement mode (mg/L or ppm) and Temperature
measurement mode in degrees Celsius (T). Other annunciators include “A”
(when the ATC function is activated), “CAL” (when meter is in calibration mode)
and battery diagram for low battery condition. See Figure 1.
milligram per litre
(Concentration Mode)
mg/l
ppm
%
TA
14-segment Liquid Crystal Display
Low Battery annunciato
r
Calibration Mode
Annunciator
CAL
Temperature Mode
A T C annunciator
parts per million
Percentage
(Percentage Saturation
Mode)
Figure 1: Active LCD display for DO 6
Instruction Manual DO 6
3
2.2 Keypad
The DO 6 meter has 6 keys on its splash-proof keypad; ON/OFF,
HOLD/ENTER CAL, MODE, ▲ and ▼ keys. Some buttons have several
functions depending on its mode of operation.
ON
OFF
ON/OFF – Powers on and shuts off the meter. The meter will start in the
measurement mode it was in when last switched off.
CAL
CAL – Activates the calibration mode for Percentage Saturation,
Concentration and Temperature calibrations.
Deactivates calibration or setup without confirming a value in calibration
mode.
HOLD
ENTER
HOLD -Activates/Deactivates freezing of the measured reading while in
measurement mode.
ENTER - Confirms the calibration values in Calibration mode and the
selections in the SETUP menu.
▲▼ – Sets the calibration values during the Concentration and
Temperature calibration.
Scrolls through each SETUP and its sub group menu.
Set offset adjustments and configuration settings.
MODE
MODE - Selects the measurement option between DO Percentage
Saturation measurement; DO Concentration measurement and
Temperature measurement.
When pressed together with ON/OFF key during power on, SETUP
mode is selected. This menu allows meter customization with
preferences such as activating ATC selection, DO concentration
measurement unit selection, viewing of the last calibration data,
viewing of the electrode properties, selecting the auto power off,
resetting calibration data or meter settings back to factory default,
setting the offset adjustments, setting barometric pressure in Hg or
PA for barometric pressure compensation and setting of the salinity
value for salinity compensation.
Instruction Manual DO 6
4
3 PREPARATION
3.1 Inserting & Removing Rubber Boot
1) To remove meter from rubber boot, push out from the bottom edges of
meter until it is completely out of boot. Ensure that the cables of Dissolved
Oxygen electrode or temperature probe are not connected.
2) To insert meter into rubber boot, slide in from the top of meter before
pushing the bottom edges of meter down to set it into position. Lift up the
stand at the back of meter for bench top applications if necessary.
Figure 1: Inserting or removing the rubber boot
Instruction Manual DO 6
5
3.2 Inserting the Batteries
The battery compartment is found at the back of instrument as shown in Figure
3. To open the battery compartment first remove protective rubber boot/stand
then:
1) Push in the direction of arrow and lift up the cover.
2) Note the polarity of battery before inserting into position.
3) After replacement, place cover back and press down until it locks tight.
Figure 2: Inserting the batteries
Instruction Manual DO 6
6
3.3 Battery Replacement
A low battery annunciator in the LCD alerts you when battery power is running
low. See Figure 4. Replace with the same type as recommended by the
manufacturer.
%
Figure 3: Low Battery Condition
Caution: Power off the meter when changing battery.
Instruction Manual DO 6
7
3.4 Dissolved Oxygen Probe Information
The DO 6 is supplied with a Dissolved Oxygen
Probe that works on galvanic principle, that is, it
does not require any polarising voltage from
your meter. Rather it will generate a millivolt
signal proportional to the amount of oxygen in
the solution.
Your DO Probe has a twin cable, one with a
BNC connector for the DO measurement input,
and the other with a phono jack plug for the
temperature measurement input. Its sensing
area consists of a cathode, anode, and an inner
electrolyte which is separated from your sample
solution by an oxygen permeable membrane
pre-membraned to the probe’s detachable cap.
Re conditioning of your probe is made easy with
the introduction of this specially designed
detachable pre-membraned cap.
This light weight probe (93X233912 /35642-50)
has a built-in temperature sensor for Automatic
Temperature Compensation (ATC) with 12mm
diameter epoxy body housing and 16 mm
diameter Noryl detachable cap. Its compact
sensing area reduces air entrapment which
makes it easy to obtain fast, accurate and
stable readings. Simply stir the probe in the
solution being measured. Shaking will aid to
remove bubbles if needed before taking a
reading. Also, always ensure to remove water
drops from the membrane when calibrating in
air.
Proper use of probe is essential to ensure that
the optimum measurement is taken in a short
Figure 4: 12 mm Galvanic DO
probe (93X233912 / 35642-50)
Detachable
Pre-membraned Cap
(Probe Sensing Area)
16 mm
12 mm
Instruction Manual DO 6
8
time. Always immerse the probe beyond the pre-membraned cap. The minimum
water flow rate is 2 inch/second across membrane. The temperature range for
use is within 0 to 50 °C.
Always ensure that the probe’s membrane is protected against any scratches or
dents. The whole pre-membraned cap has to be replaced if the membrane is
damaged. It is also important to keep the membrane clean so as to produce an
optimum and accurate measurement.
See Section 8 - “Probe Care and Maintenance” for more information.
Instruction Manual DO 6
9
3.5 Conn ecting the Probe to Meter
1) Insert the BNC connector from the electrode to the BNC connector socket
on the meter accordingly and rotate connector clockwise until it locks. See
Figure below.
2) To remove, simply rotate the connector in counter-clockwise direction until
it unlocks, and slide the connector off the socket.
3) Plug the phono jack of temperature sensor into the phono socket of the
meter as shown in Figure below.
Caution: Do not pull or force the probe cord or the probe wires might
disconnect.
Note: Keep connectors clean. Do not touch connector with soiled hands.
BNC Connector for
DO probe
Phono jack for
Temperature probe
Figure 5: DO 6 probe to meter connections
Instruction Manual DO 6
10
3.6 Switching the Meter On
When switching the meter on, it will go
through a series of display, eventually
showing the revision number of the meter
before going to the measurement mode.
The mode of measurement which the
meter displays will be similar to the last
measurement mode to which the meter is
switched off.
Press ON/OFF key to power up your
meter.
1) All LCD segments will light up for 1.5
seconds.
2) The display will then momentarily
show the meter’s identification “DO 6”
with the annunciators for percentage
saturation mode and concentration
mode.
3) The display continues to switch to
display meter’s revision number for
1.5 seconds before finally showing
the measurement mode.
4) Either percentage saturation
measurement mode, concentration
measurement mode or temperature
measurement mode will be displayed
following the previous measurement
mode to which the meter is switched
off.
CAL 13 CAL 24
mg/l
NTU
ppm
%pH
TAvg
mg/l
ppm
%
ON
OFF
A
%
Figure 6: Power Up sequence
Instruction Manual DO 6
11
3.7 Measur ement Mode Selection:- Percentage
Saturation (%), Concentration (mg/L)(ppm) and
Temperature(T)
By simply pressing the MODE key, you can select the measurement mode of
percentage saturation measurement, concentration measurement or
temperature measurement.
The customized annunciator shall indicate the selection of the measurement
mode.
mg/l
A
TA
A
%
MODE
MODE
MODE
Percentage Saturation
Concentration Mode
Temperature Mode
Automatic Temperature
Compensation
Figure 7: Measurement mode selection using the mode key
Instruction Manual DO 6
12
4 CALIBRATION
4.1 Importan t Information on Meter Calibration
Your meter has three measurement modes namely the DO % saturation
measurement mode, the DO mg/L (ppm) concentration mode and the
Temperature measurement mode.
Since the mg/L (ppm) concentration value of dissolved oxygen varies with
temperature, barometric pressure and salinity, the meter with its electrode has
to be calibrated with consideration to these factors to ensure a proper
measurement. Therefore, it is very important to set the proper temperature,
barometric pressure and salinity values in the meter prior to any calibration or
measurement process. Since the dissolved oxygen measurements in %
saturation will linearly affect the mg/L (ppm) concentration measurement, it is
important that the meter has to be first calibrated in the % Saturation mode and
temperature mode before commencing the mg/L (ppm) concentration mode
calibration.
In % saturation calibration mode, the dissolved oxygen calibration values will
vary according to the barometric pressure correction set in the meter. Therefore
it is necessary to set the barometric pressure first before proceeding to %
Saturation calibration. The following table is an example which explains the
acceptance calibration values when % Saturation mode calibration is attempted
under different absolute value and under two different barometric pressures.
% Saturation factory
default value
Acceptance Calibration Value
(760mmHg)
Acceptance Calibration Value
(700mmHg)
less than 10% 0% 0%
10.1% to 49.9% Err.1 (error 1) Err.1 (error 1)
50% to 200% 100% 92.1%
Instruction Manual DO 6
13
In the mg/L (ppm) concentration calibration mode, barometric pressure, salinity
and temperature of the calibration sample needs to be set in the meter prior to
calibration. Since the pressure has already been set in the % saturation
calibration which is done earlier and assuming that the calibration is done on
the same barometric pressure and if you are using the temperature sensor in
automatic temperature compensation mode, you only need to input the known
salinity of the calibration sample to the meter. The dissolved oxygen mg/L (ppm)
concentration value can then be calibrated to the known sample concentration
calibration value. Note that the concentration calibration window is +/- 40% of
the factory default value. However the minimum point of calibration is 2 mg/L.
When you recalibrate your meter in % saturation mode, old calibrations in %
saturation will be replaced while the % saturation offset adjustment will be
erased. However, recalibration in the mg/L (ppm) concentration mode will only
replace the old calibration in the concentration mode and do not affect the
calibration in the percentage saturation mode.
To completely recalibrate your meter, or when you use a replacement electrode,
it is best to clear all calibration data. To erase all the old calibration data
completely, see Section 7.6 (rSt.5) Reset to Factory Default
4.2 Preparing th e Meter for Calibration
Before starting calibration, make sure you are in the correct measurement mode
and in the correct calibration sequence. The temperature and the % Saturation
calibration must be done first before attempting to do the mg/L (ppm)
Concentration calibration.
In % Saturation, the meter is able to perform either a one point calibration or a 2
point calibration. For one point calibration, it is recommended that you perform a
100% Saturation calibration in saturated air. If you opt for 2 point calibration,
you can calibrate for 100% Saturation in saturated air and 0% Saturation using
a zero oxygen solution. The meter will take several minutes to reach 0%
Saturation value after submersion into the zero solution.
Rinse the probe well in the de-ionized (DI) water or rinse solution and wipe the
probe carefully taking care of the membrane.
Calibrate the meter in all the modes to ensure the highest accuracy throughout
the DO measurement range. In % Saturation calibration, should there be a
Instruction Manual DO 6
14
calibration failure for 0% Saturation slope calibration; the meter may have
exceeded the limit of 10% of the factory calibrated absolute value. The sensor
may have to be re- conditioned. Also, note that the DO 6 meter will not perform
0% or 100% Saturation calibration for absolute value ranging from 10.1% to
49.9%. The meter also will not perform the mg/L (ppm) Concentration
calibration for an absolute value of less than 2.00 mg/L (ppm) or calibration
which is out of its window tolerance of +/- 40% from the factory default value.
An error message of “Err.1” will be indicated and will return the meter to the
measurement mode.
All new calibration values will automatically override the existing data. It is
recommended to calibrate the meter periodically and or if it is suspected to be
inaccurate.
Always rinse the probe with either DI water or rinse solution before and after
each calibration/sample measurement. When calibrating in air, make sure that
any water droplets from the probe’s membrane are removed.
For details please refer to Section 8 - PROBE CARE AND MAINTENANCE.
Note: The DO6 factory calibrated default value is in respect to 760mm Hg or
101.3 kPa barometric pressure (sea level). To set the barometric pressure to
different value, see Section 5.3.1 Pressure Setting Adjustment.
Instruction Manual DO 6
15
4.3 Temperature Calibration
DO mg/L (ppm) Concentration measurement
is dependent on the temperature of the
sample to be measured. Most users will
choose to have the DO6 automatically
compensate for temperature using the
temperature sensor that is built into the DO
probe. However, it is possible to manually
enter a known temperature to the meter
(manual temperature compensation). The
built-in temperature sensor of the DO6 probe
is factory calibrated. However, the
temperature can be re-calibrated if needed
and is recommended when a replacement
probe is used.
Before calibration, ensure that the meter
displays annunciator “A” to indicate that it is
in automatic temperature compensation
mode. Please see Set Up menu on page 36
for its activation procedure.
1) Switch the meter on. Press MODE to
select temperature mode. The display
should show the annunciator “T” for
temperature mode and “A” for automatic
temperature compensation mode.
2) Dip the probe to a solution of known
temperature (i.e. a temperature
bath).Allow some time for the reading to
stabilize.
3) Press the CAL key. The CAL indicator
will appear and blinks above the display.
The temperature value displayed is with
respect to the factory default calibration.
CAL
CAL
TTA
A
TA
TA
CAL
HOLD
ENTER
Factory default
Temperature
Measurement
“CO” will display for
1.5 seconds
Figure 8: Temperature Calibration Sequ ence
Instruction Manual DO 6
16
4) Press the ▲ and ▼ keys to adjust the meter reading to the correct
temperature value (i.e. the temperature of the temperature bath)
5) Press the HOLD/ENTER key to confirm temperature calibration and return
to measurement mode.
NOTE:
To exit from Temperature Calibration mode without confirming the calibration,
press CAL instead of HOLD/ENTER.
Temperature calibration is restricted to ± 5°C from the factory default
temperature measurement displayed during calibration.
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