EST Centurion W, Centurion WS Operating Instructions Manual

Centurion W and WS Manual
Original Operating Instructions
Rev. 7.6

Notices

No part of this manual may be reproduced in any form or by any means (including electronic
storage and retrieval or translation into a foreign language) without prior agreement and written
consent of EST Analytical.

Version

Rev. 7.6

Printed in USA

EST Analytical
503 Commercial Dr.
Fairfield OH, 45014 USA

Acknowledgements

Encon® is a registered trademark of EST Analytical
Tekmar® LSC2000 and Tekmar® LSC3000 are registered trademarks of Teledyne
Technologies, Inc.
OI®4460, 4560 and 4660 are registered trademarks of OI Analytical, Inc.
Windows®.Net is a registered trademark of the Microsoft Corp.

Trademark information is accurate to the best of our knowledge at the time of printing.

The symbol marked on the product indicates compliance with the requirements of
CAN/CSAC22.2 No.61010.1, 2nd edition, including Amendment 1.

Warranty

The material contained in this document is provided “as is”, and is subject to being changed
without notice, in future editions. EST Analytical warrants the products it manufactures and
distributes, except those specially exempted, to be free from defects for (1) full year from the
date of shipment. This warranty is limited to the original purchaser of the product and is not
transferable. This limited warranty does not extend to any products that have been damaged as a
result of accident, misuse, abuse, or service or modification by anyone.

Except as expressly set forth above, no other warranties are expressed or implied including, but
not limited to, any implied warranties merchantability and fitness for a particular purpose, and
EST expressly disclaims all warranties not stated herein. In the event the product is not free
from defect as warranted above, the purchaser’s sole remedy shall be provided above. Under no
circumstances will EST Analytical be liable to the purchaser or any user for any damages,
including the incidental or consequential damages, expenses, lost profits, lost savings or other
damages arising out of the use or inability to use the product. This warranty shall not be
applicable to the extent that any provision of this warranty is prohibited by any federal, state, or
municipal law that cannot be preempted.

Federal Communications Commissions advisory:

This equipment has not been tested or found to comply with the limits of Class A computing
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable
protection against harmful interference when the equipment is operated in a commercial
environment. This equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful interference to
radio communications. Operation of this equipment in a residential area is likely to cause
harmful interference in which case the user will be required to correct the interference at his
expense.

CAUTION

A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or
the like that, if not properly performed or adhered to, could result in damage. Do not proceed
beyond a CAUTION notice until the indicated conditions are completely understood and met.

ATTENTION

La mention ATTENTION signale un danger. Il attire l'attention sur une procédure
d'exploitation, la pratique ou la comme ça, s'il n'est pas correctement effectuée ou respectée, peut
entraîner des dommages. Ne pas aller au-delà d'une mention ATTENTION jusqu'à ce que les
conditions indiquées sont complètement comprises et respectées.

WARNING

A WARNING notice denotes a hazard. It calls attention to an operating procedure or practice,
or the like that, if not properly performed or adhered to, could result in personal injury of death.
Do not proceed beyond a WARNING notice until the indicated conditions are completely
understood and met.

AVERTISSEMENT

La mention AVERTISSEMENT signale un danger. Il attire l'attention sur une procédure ou une
pratique exploitation, ou le comme ça, s'il n'est pas correctement effectuée ou respectée, peut
entraîner des blessures de la mort. Ne pas aller au-delà d'une mention AVERTISSEMENT
jusqu'à ce que les conditions indiquées sont complètement comprises et respectées.

Introduction
…………………………………………………………Chapter 1

1.1 Product Description

1.2 Design Features

1.3 Product Specifications

1.4 Warnings and Symbols

1.5 Disposal and Recycling Information

Centurion W/S Installation and Setup
…………………………………………………………Chapter 2

2.1 General Information

2.2 Work Space Requirements

2.3 Power Requirements

2.4 Unpacking the Centurion

2.5 Centurion Setup/Installation

2.5.1 Connections on the Back of the Centurion

2.5.2 Water Reservoir and Cap Assembly

2.5.3 Feed Gas Installation

2.5.4 Interface Connection

2.5.5 Monitor Setup

2.5.6 Power Connection

2.5.7 Vial Storage Trays

2.5.8 Internal Standard Reservoir Installation

2.5.9 Pressurize System

2.5.10 Instrument Start-up

2.5.11 Water and Soil Axis Calibration

2.5.12 Method Build

2.5.13 System Set-up Complete

Centurion W/S Operation
…………………………………………………………Chapter 3

3.1 Powering on Centurion W/S

3.2 Opening the Centurion W/S Application

3.3 Powering off the Centurion W/S

3.4 Main Screen

3.5 Diagnostics Screen

3.6 Home/Logon Screen

3.7 Exit Icon

3.8 Options Screen

3.8.1 Instrument Tab

3.8.2 System Setup

3.8.3 User Accounts

3.9 Sequence Screen

3.9.1 Concentrator #1

3.9.2 Populating the Sequence Table

3.9.3 Run Setup

3.10 Method Screen

3.10.1 General Setup

3.10.2 Internal Standards Tab

3.10.3 Soil Sample Tab (OPTION)

3.11 Run Status Screen

3.12 Run Log Screen

3.13 Help Screen

User Maintenance and Troubleshooting
……………………………………………………………Chapter 4

4.1 Technical Support Contact Information

4.2 Cleaning the Centurion W/S

4.3 Filling the Water Reservoir

4.4 Diagnostics Screen

4.4.1 E-Stop

4.4.2 Flow Pathway Dropdown Menu

4.4.3 Icons

4.4.4 Vial Position

4.4.5 Manual Heater Control

4.4.6 Manual Movement Control

4.5 Manual Functions

4.5.1 Rinse Functions

4.5.2 Drain Sparge Tube

4.5.3 Internal Standards

4.5.4 Sweep Soil Needle

4.5.5 Mechanical Test

4.5.6 Syringe Maintenance

4.6 Calibration Functions

4.6.1 Auto Calibration

4.6.2 Manual Calibration

4.6.3 Syringe Calibration

4.6.4 Heater Calibration

4.6.5 Digital Input/Output

4.6.6 Internal Standard Calibration

4.6.7 Stirrer Calibration

4.7 Leak Checking the Soil Pathway

4.8 Replacement of Sampling Station Needle

4.9 Removal/Installation of Water Needle

4.10 Fuse Replacement

4.11 Spare Parts

Flow Diagrams
……………………………………………………………Chapter 5

Appendix A Instructions for the Water and Methanol Extraction

Feature

Appendix B Installation of the Centurion W or WS to an OI 4660

Concentrator

Appendix C Installation of the Centurion W or WS to a Tekmar

Stratum Concentrator

Appendix D Installation of the Centurion W or WS to a Tekmar

3000/3100 Concentrator

User Manual

Introduction

Chapter 1

1.1 Product Description

The EST Centurion W and WS Purge and Trap Autosamplers are designed to automate
the tedious sample handling procedures associated with purge and trap analysis for
volatile organic compounds (VOC's) under current EPA methods. The EST Centurion
can be used for water and soil (optional) matrices and is designed as a stand-alone unit
for retrofit to any manufactured purge and trap concentrator. Laboratories in today’s
market have enormous challenges to reduce cost and improve productivity. The EST
Centurion provides the flexibility to meet these needs.

The EST Centurion W and WS are a state-of-the art robotic, X-Y-Z axis autosampler
with a unique Windows XPe™ operating platform. The Autosampler has 100 sample
positions, (90 soil), with 2 removable 50 position trays. A vial cooling option is available
which allows the vials to be cooled to below 10°C.

To process a water sample, the 40ml VOA vial is placed in the tray. The Centurion W or
WS robotic arm moves to the programmed vial location and the sample needle is lowered
into the sample vial to a calibrated depth. The sample vial is pressurized, transferring the
sample from the vial through an inert pathway filling a fixed volume sample loop. The
overflow is carried out to waste. The internal standard is injected into the sample stream
and the sample is transferred to the sparge vessel located on the concentrator.

The syringe option for the Centurion W or WS autosampler allows automated sample
dilutions. The dilution range is from 2x to 400x. The Centurion W or WS robotic arm
moves to the vial location as described previously. Next, an aliquot of sample is pulled
from the vial and diluted using the chosen dilution factor, the sample is then sent through
an inert sample pathway, injected with internal standard and transferred to the sparge
vessel on the concentrator. The syringe option also provides the capability to sample
volumes from 5mls to 25mls in 1ml increments.

To process a soil sample, the 40ml VOA vial is placed in the tray. The Centurion WS
robotic arm moves to the programmed vial location. Unlike the water vial which stays in
the sample tray, in the soil mode, the vial is transported to the soil cup. In the soil cup,
internal standard can be transferred into blank water through the fixed volume loop to the
sample. The sample can then be heated, stirred and purged to your specifications.
Finally, the analytes purged out of the sample are transferred to the concentrator through
a heated transfer line.

The Centurion W or WS autosampler will rinse the sample pathway with hot water for
cleaning in preparation of the next sample. The number of rinses and rinse time for the
sample needle and sample loop are programmable. The Centurion W or WS can have two
distinct modes of operation. They are the Single Concentrator and Dual Concentrator
(optional) modes. The single concentrator mode will allow the vial autosampler to run
with one purge and trap concentrator. The optional Dual concentrator mode will allow the
Centurion W or WS autosampler to process and deliver water and soil samples to two
purge and trap concentrators on an efficient alternating basis. The Dual Mode

significantly increases the sample throughput of the Autosampler. There is no need to
purchase a second autosampler. All modes perform per their programmed method.

The EST Centurion W or WS is easily programmed through their own keyboard and
mouse. The Centurion W or WS has two USB ports for the keyboard and mouse.
Programming is menu driven and displayed on a color monitor. Parameters can be read
and altered and the screen displays the function in progress during the run.

The Centurion W or WS when connected to an EST Encon Evolution concentrator
further enhances cost savings and improves sample throughput with the Foam Sensor
option on the Evolution. The Centurion W or WS works with the Evolution to rinse the
sparge tube with a pre-programmed number of hot water rinses whenever a foaming
sample is detected. This option prevents the accidental transfer of a foamed sample to the
GC column which can result in down time and costly repairs.

NOTE: For clarification purposes, the use of the word Centurion throughout the
manual shall refer to both the Centurion W and WS.

1.2 Design Features

 Dual 50 position removable sample trays for 40ml VOA vials.
 Rugged X-Y-Z platform.
 Windows XPe™ operating platform.
 Runs blanks from its own blank/wash water reservoir.
 Sample Loop sizes range from 5, 10, 15, 20 or 25ml.
 Syringe Option, allows dilutions from 2x to 400x and sample sizes from 5mls to 25mls

in 1ml increments.

 All sample pathways are chemically inert, manufactured from PEEK®, Stainless Steel,

or Teflon.

 Separate needles for the soil and water modes thus minimizing carryover between the

two matrices.

 Built in Minimizer Valve aiding in the removal of contamination on the soil side by

diverting some of the bake flow from the concentrator through the soil pathway of the
Centurion.

 Programmable volume addition of Internal Standards/Surrogates.
 Optional sample tray cooling capability. Optional refrigerated chiller is required.
 Priority Sample feature allows the current analysis to be interrupted for RUSH

samples.

 Fast easy programming from the keyboard/mouse combination.
 Programmable methods may be stored for various method configurations.
 A sample may be run from any position in the tray.

 Easy hook up and installation to all manufactured purge and trap systems.
 Hot water rinse utilizes a cartridge heater to heat blank water to 90C prior to flushing

sample pathways and the purge vessel.

 Optional Dual concentrator mode for both water and soil matrices.
 Optional Dual GC mode.

1.3 Product Specifications

Tray Capacity:

100 sample vials. 40 ml EPA VOC

Sample Volume:

Indoor Use
Altitude:
Operating

Temperature:
Relative Humidity:
Voltage:

Current:

Battery:
Installation Category:

Pollution Degree:

5, 10, 15, 20, 25ml sample loop, 5 to 25ml volume
syringe (1ml increments for syringe)

Up to 2000 m
15C to 25C; Storage Temperature 5C to 85C

10 to 90%
115/230VAC ( 10%), 50/60Hz

Model

CenturionW 4.0 2.0

CenturionWS 8.0 5.0

115VAC 230VAC

Current(A) Current(A)

CR2032 battery contained on SBC (CPU) board
II

2

Weight:
Size:
Gas Requirements:

Approximately 80 lbs; 36.5 Kilograms

24.5”W (62.2 cm) x 27”D (68.6 cm) x 25” H (63.5 cm)
Helium or Nitrogen 60-80 PSI (414-552kPa) 99.999%

GC/MS grade purity; BTU/hr. = NA (compressed gas
used for pressurization only)

1.4 Warnings and Symbols

English

 Warnings in the manual or on the Centurion to be observed during installation, operation,

service and/or repair of the instrument. EST Analytical will assume no responsibility for
customers failing to comply with the safety precautions stated.

 Use of the Centurion for something other than the intended use of the instrument is

prohibited.

 The use of the Centurion is for trained personnel only.
 Personnel operating the Centurion should wear safety glasses and gloves where

appropriate.

 The Centurion is not intended for use in a hazardous environment.
 Installation of the Centurion requires that there is nothing blocking the vents of the

system.

 Installation of the Centurion requires that the instrument be placed on a non-flammable

surface capable of handling the weight of the instrument.

 Heaters and heated transfer line(s) require surrounding space to be non-combustible

material.

 Sound pressure level of the Centurion is 65db (A).
 When cleaning the Centurion please refer to section 4.2 of the User Manual. No other

cleaning agents may be used other than what is specified in this section.

Français

 Les avertissements dans le manuel ou sur le Centurion à respecter lors de l'installation , le

fonctionnement , le service et / ou la réparation de l'instrument . EST analytique n'assume
aucune responsabilité pour les clients qui ne respectent pas les consignes de sécurité
indiquées .

 Utilisation du Centurion pour autre chose que l'utilisation prévue de l'instrument est

interdite.

 L'utilisation du Centurion est de personnel qualifié seulement .
 Le personnel d'exploitation du Centurion doivent porter des lunettes de sécurité et des

gants le cas échéant .

 Le Centurion n'est pas destiné à être utilisé dans un environnement dangereux .
 L'installation du Centurion exige que rien ne bloque les évents du système .
 Montage du Centurion nécessite que l'instrument est placé sur une surface non

inflammable capable de supporter le poids de l'instrument.

 Chauffe et ligne de transfert chauffée (s) nécessitent de l'espace environnant à un

matériau incombustible .

 le niveau de la Centurion de pression sonore est de 65db ( A) .
 Lors du nettoyage du Centurion s'il vous plaît se référer à la section 4.2 du Manuel de

l'utilisateur . Pas d'autres agents de nettoyage peuvent être utilisées autrement que ce qui
est spécifié dans cette section.

See Accompanying Instructions for more Information

Voir les instructions d'accompagnement pour plus d'informations

Indicates an Electrical Hazard

Indique un danger électrique

Indicates Hot Surface

Indique surface chaude

Indicates a Risk of Hand Entanglement

Indique un risque d'étranglement pour les mains

Indicates a Sharp Point

Indique une pointe acérée

Indicates Bulky/Heavy Item, Use Two Person Lift

Indique objet volumineux / lourds, utilisez deux personnes ascenseur

1.5 Disposal and Recycling Information

United States:
This symbol indicates that your product must be disposed of properly according
to local laws and regulations. When your product reaches its end of life, contact
your local authorities to learn about recycling options.

European Union:
This symbol above means that according to local laws and regulations your product should be
disposed of separately from household waste. When this product reaches its end of life, take it to
a collection point designated by local authorities. Some collection points accept products for
free. The separate collection and recycling of your product at the time of disposal will help
conserve natural resources and ensure that it is recycled in a manner that protects human health
and the environment.

User Manual

Installation and Setup

Chapter 2

2.1 General Information

The system setup and installation section is intended to be as thorough as possible. If in­doubt at any point, please contact EST Analytical toll free at [phone]. If
installation is not directed or performed by EST Analytical personnel, the operator must
be thoroughly familiar with setup and installation sections of this manual.

NOTE: Failure to follow the installation instructions as set forth could result in
damage to the product and nullify the warranty.

2.2 Work Space Requirements

The Centurion foot print is 24.5”W (62.2 cm) x 27”D (68.6 cm) x 25” H (63.5 cm).

NOTE: Allow for added space on all sides of the instrument for proper ventilation.

2.3 Power Requirements

Voltage:
115/230 VAC (+/- 10%)
50/60 Hz

8.0/5.0 Amps
15/20A circuit breaker

2.4 Unpacking the Centurion

Figure 2a: Shipping Container

 The Centurion is packaged in one Carton containing all components necessary for

instrument installation and operation.

NOTE: Retain all shipping material.

 Before un-strapping the Centurion Box, visually inspect the shipping carton for any

damage. Call EST Analytical at [phone] immediately if visual damage is present.

 Cut the strapping and lift the top box cover off the box and put aside. Lift the outer box

shell off the Centurion and put aside. Remove the excess foam packing material from the
top of the Centurion and put aside.

Figure 2b: Instrumentation Packing 1

 Remove the two boxes inside of the Centurion main body. Box #1 contains the vial

storage trays. Box #2 will contain the installation kit, I/O cable, keyboard, mouse,
internal standard vessel, and power cord.

Figure 2c: Instrumentation Packing 2

 With the help of another person tilt the Centurion on one side and remove the

foam inserts. The Centurion in now ready to place on the bench. With one person on
EACH side of the Centurion transfer the Centurion to the bench.

NOTE: The Centurion needs to be lifted from the base of the instrument.
WARNING: The Centurion needs enough space on all sides of the instrument to
ensure proper cooling of instrument components AND to ensure that the power cord
can be easily disconnected.

AVERTISSEMENT: Le Centurion besoin de suffisamment d'espace sur tous les
côtés de l'appareil pour assurer le bon refroidissement des composants de
l'instrument et de veiller à ce que le cordon d'alimentation peut être facilement
déconnecté.

2.5 Centurion Setup/Installation

2.5.1 Install all connections at the rear of the instrument:

 Keyboard-grey USB cable, see Figure 2d.
 Mouse-white USB cable, see Figure 2d.

Figure 2d: Centurion Keyboard, Mouse and Interface Connections

 Waste (W)-Black Tubing, see Figure 2e.

o Flangeless Nut-Green
o Flangeless Ferrule-PEEK

Figure 2e: Waste Line

 Concentrator (1)-PEEK Tubing, see Figure 2e and 2f

o Super Flangeless Nut-black
o Super Flangeless Ferrule-ring/PEEK ferrule
o Connect to Concentrator (1) three way valve-green nut and

natural ferrule.

Figure 2f: Water Transfer Line Connection to Concentrator

 Concentrator (2)-if applicable, same connections as Concentrator (1).
 Concentrator (1)-Soil Purge Gas Line Connections – If Applicable.

(Note: Shipped as an assembly.)

o Connect PEEK tubing (red) to Concentrator (1) purge bulkhead

with Upchurch nut and Ferrule. See Figure 2g.

Figure 2g: PEEK Lines from Centurion to Encon Evolution

o Connect other end of the PEEK tubing (red) to the Centurion WS

port labeled Concentrator (1) Purge Bulkhead using Reverse Nut
PEEK and ferrule. See Figure 2h.

Figure 2h: PEEK lines on the back of the Centurion

o Connect PEEK tubing (blue) to Concentrator (1) glassware on the

purge side using the 1/16-1/4 Union. See Figure 2g.

o Connect the other end of the PEEK tubing (blue) to the Centurion

WS port labeled Concentrator (1) Sparge Tube using Reverse Nut
PEEK and ferrule. See Figure 2h.

 Concentrator (2)-if applicable, same connections as Concentrator (1).
 Soil Transfer Line

o Connect Valco nut on the end of the heated transfer line to the four

way Tee in the concentrator. See Figure 2i.

WARNING: The heated transfer line needs to be routed at least
10 inches away from any adjacent objects. All other cables need to
be routed away from the transfer line heaters also.

AVERTISSEMENT: La ligne de transfert chauffée doit être
acheminé au moins 25.4 centimètres de tous les objets adjacents.
Tous les autres câbles doivent être acheminés à l'écart des
réchauffeurs de conduites de transfert aussi.

 Pressure Line (Water Bottle)-Black tubing.

o Quick connect fitting to back panel. See Figure 2j

Figure 2j: Water Bottle Pressure Line, Cold and Hot Water Lines

Figure 2i: Soil Transfer Line

 Cold Water Input (C)-Teflon Tubing, See Figure 2j

o Flangeless Nut-Green
o Flangeless Ferrule-PEEK

 Hot Water Input (W)-Teflon Tubing, See Figure 2j

o Flangeless Nut-Green
o Flangeless Ferrule-PEEK

2.5.2 Water Reservoir Cap Assembly

 Install Nut and Ferrule on the reservoir end of the cold and hot water

pickup lines. See Figure 2k.

o Flangeless Nut-Green
o Flangeless Ferrule-PEEK

 Install cold and hot water pickup lines through two of the three holes in

the water reservoir plug. Lines need to reach the bottom of the reservoir.

 Install the frits to the ends of the cold and hot water pickup lines after

they have been installed through the reservoir plug. See Figure 2l.

o Frit

Figure 2k: Reservoir Lines

Figure 2l: Water Line Frits

 Fill reservoir with organic free water.
 Install o-ring onto the smaller flange end of the reservoir plug
 Install the black pressure line into the cap allowing the end of the line to

come through the hole and into the reservoir about 3-5mm deep or cut
flush with the bottom of the cap insert.

 Screw the cap onto the reservoir. See Figure 2m.

Figure 2m: Reservoir Bottle

2.5.3 Feed Gas Installation

 Install a Tee to the end of the feed gas line and Tee in the feed gas to the

Centurion W/S with the other end of the Tee going to the concentrator.

NOTE: Make sure the pressure is between 60-80psi (414-552kPa).

2.5.4 Interface Connection

 Install supplied interface cable between Centurion and Concentrator. In

Single Concentrator Mode the Centurion is equipped with a 25 pin
connector which connects to Concentrator (#1). If the Centurion is
equipped with the optional Dual Mode the interface cable will have a
second 25 pin male connector that will connect to Concentrator (#2) in
the same manner as Concentrator #1. The Centurion can be interfaced
with different manufactured concentrators and or multiple GC’s. These
configurations require a custom cable. See Appendix A for the specific
instructions on installation to other concentrators. Call EST at
[phone] if you have any question about your interface connections.
See Figure 2d.

2.5.5 Monitor Setup

 Mount the monitor to the mounting bracket with the four screws given in

the Monitor box. Plug the VGA cable to the VGA port on the back of the
monitor. Plug power cable to the 24Vdc power port on the back of the
monitor as shown below in Figure 2n.

Figure 2n: Monitor Connections

2.5.6 Power Connection

 Plug the power cord into the Power Entry module on the back of the

Centurion. See Figure 2o.

WARNING: Ensure proper grounding of the Centurion system
before powering the system up.

AVERTISSEMENT: S'assurer de la bonne mise à la terre du système
Centurion avant de mettre le système en place.

Figure 2o: Power Entry Module and Helium Input Connector

2.5.7 Vial Storage Trays

 Place the vial storage trays onto the base of the Centurion. The trays have

a key on the bottom side which will fit the slots on the base of the
Centurion. See Figure 2p.

Figure 2p: Vial Storage Tray

2.5.8 Internal Standard Reservoir Installation

 Rinse and fill IS reservoir(s) with methanol.
 Place the knurled nut(s) and teflon ferrules on the reservoir(s) and tighten

the IS reservoir(s) in the respective position(s). NOTE: Tighten until
finger tight and use the provided IS mount wrench to tighten the IS
vessel about ¼ of a turn more. See Figure 2q. NOTE: The system
must be de-pressurized in order to remove the IS reservoir.

2.5.9 Pressurize System

 Turn on the pressurization valve on the back panel of the Centurion WS.

See Figure 2r.

 Adjust the system pressure on the front panel to 14-15psi (97-103kPa). If

the pressure is set too high it may be necessary to back of the regulator
and release the pressure by turning off the pressurization valve. In order
for the gauge to drop in pressure, an IS mount may need to be loosened.
Allow the pressure to bleed off of the system, the re-pressurize the system
and adjust the front panel system pressure to the required set-point.

Figure 2q: Internal Standard Vessels

2.5.10 Instrument Start-up

 Turn on the instrument.
 Login-Password for the Administrator is ESTAnalytical (Case Sensitive).
 Wait for the IO system configuration to load; then open the Application.

Figure 2r: System Pressure Switch

 Proceed to the Diagnostics screen and prime the IS vessel(s) three times

with methanol. NOTE: Refer to section 4.5.3 of the Diagnostics
Chapter on how to prime the IS vessel(s).

 De-pressurize the system, remove the IS vessel(s) and rinse and re-fill

them with your required IS and place them back on the system.

 Re-pressurized and prime a second time with the IS.

2.5.11 Water and Soil Axis Calibration

 Perform a Mechanical Test; refer to section 4.5.5 in the User

Maintenance and Troubleshooting Chapter.

 If the system fails the Mechanical Test, the water and soil axis will need

to be calibrated. Refer to section 4.6.1 for instructions on how to auto
calibrate the Centurion.

2.5.12 Method Build

 Setup a user method by setting all times. NOTE: Make sure that

the set desorb time on the Centurion WS method is the SAME as the
desorb time of the concentrator.

 Use the water waste line to monitor if the sample fill time has been set

correctly.

 Verify the water sample delivery time by evidence of gas flow into the

sparge tube after complete water delivery.

 Refer to Table 2a for typical Method settings for the WS with a 5ml loop.

Purge and Trap Auto-Sampler EST Centurion WS

Sample Type Water

Sample Loop Fill Time (sec) 12

Loop Equilibration Time (sec) 5

Sample Transfer Time (sec) 15

Needle Rinse Time (sec) On/10

Needle Sweep Time (sec) 5

Sample Loop Rinse Time (sec) On/10
Sample Loop Sweep Time (sec) 5
Concentrator Desorb Time (sec) 60

Sparge Rinse Time (sec) On/25
Sparge Rinse Transfer Time (sec) 20
Concentrator #1 Cycle Time (min) 0

Number of Rinse Cycles 2
Number of Foam Cycles 1

Water Heater Temp. (°C) 85

Table 2a: Typical Centurion Water Parameters for a 5ml Loop

2.5.13 System Set-up is Complete

You are now ready to run.

User Manual

Operation

Chapter 3

3.1 Power On

1. Please read the entire manual and become familiar with the operation of the

Centurion prior to installation of the autosampler. NOTE: Only a trained operator
may run the Centurion.

2. Be sure that all electrical connections are in place and the plumbing connection s, both

water and gas, are free of leaks.

3. Check all waste lines and ensure that they are properly routed and contained before

powering on the autosampler.

4. Press ON at the power switch located on the right side of the rear panel.

5. The embedded Windows XPe

™ operating system will load and the IO System

Configuration will load. The IO System Configuration is a self-loading
communication device between the Centurion and the Windows XPe™ operating
system.

3.2 Open the Centurion Application

1. The user will control the operation of the Centurion with a mouse and keyboard.

2. <click> the Centurion icon on the desktop.

3. The EST Analytical screen will appear while the application is loading.

4. After the application has loaded the user will be prompted to log on with a password

as shown in Figure 3a. NOTE: The password for the Centurion is ESTAnalytical
(case sensitive).

5. The user will then be prompted to Home All Axes as shown in Figure 3b. The

Centurion must be homed prior to operation.

Figure 3a: Log in Screen

Figure 3b: Home Axes Screen