Emerson Rosemount 550pH Reference Manual

00809-0100-3550, Rev AA

Rosemount™ 550pH

Single-use pH Sensor for Bioprocessing Applications

Reference Manual

March 2019

Essential instructions

Read this page before proceeding!

Emerson designs, manufactures, and tests its products to meet many national and international standards. Because these
instruments are sophisticated technical products, you must properly install, use, and maintain them to ensure they continue to
operate within their normal specifications. The following instructions must be adhered to and integrated into your safety program
when installing, using, and maintaining Emerson products. Failure to follow the proper instructions may cause any one of the
following situations to occur: loss of life, personal injury, property damage, damage to this instrument, and warranty invalidation.

• Read all instructions prior to installing, operating, and servicing the product.

• If this Reference Manual is not the correct one, call 1-800-999-9307 to request the correct Reference Manual. Save this

Reference Manual for future reference.

• If you do not understand any of the instructions, contact your Emerson representative for clarification.

• Follow all warnings, cautions, and instructions marked on and supplied with the product.

• Inform and educate your personnel in the proper installation, operation, and maintenance of the product.

• Install equipment as specified in the installation instructions of the appropriate Reference Manual and per applicable local and

national codes. Connect all products to the proper electrical and pressure sources.

• To ensure proper performance, use qualified personnel to install, operate, update, program, and maintain the product.

• When replacement parts are required, ensure that qualified people use replacement parts specified by Rosemount.

Unauthorized parts and procedures can affect the product's performance, place the safe operation of your process at risk, and
may result in fire, electrical hazards, or improper operation.

• Ensure that all equipment doors are closed and protective covers are in place, except when maintenance is being performed

by qualified people, to prevent electrical shock and personal injury.

2

Reference Manual Contents

00809-0100-3550 March 2019

Contents

Chapter 1 Sensor readiness............................................................................................................ 5

1.1 Storage and handling....................................................................................................................... 5

1.2 Specifications................................................................................................................................... 5

1.3 Dimensional drawings...................................................................................................................... 7

Chapter 2 Install the sensor............................................................................................................9

Chapter 3 Wire the sensor............................................................................................................13

Chapter 4 Calibrate...................................................................................................................... 17

4.1 One-point standardization based on a grab sample........................................................................ 17

Chapter 5 Troubleshoot............................................................................................................... 19

5.1 Faulty reading.................................................................................................................................19

5.2 Label information too small............................................................................................................ 19

Chapter 6 China RoHS table..........................................................................................................21

Chapter 7 EU Declaration of Conformity....................................................................................... 23

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Contents Reference Manual

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4 Rosemount 550pH

Reference Manual Sensor readiness

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1 Sensor readiness

1.1 Storage and handling

Store the sensors attached to the single-use bioreactor until needed.

WARNING

Do not allow sensors to freeze or go below 39 °F (4° C).

Disposal

If the sensors have been contaminated, decontaminate prior to disposal. Please dispose
with other electrical and electronic waste per local guidelines. Declarations of Conformity
can be found at the end of this manual.

1.2 Specifications

Table 1-1: Rosemount™ 550pH Sensor Specifications

Specifications Materials and units

Operating temperature range 39 to 104 °F (4 to 40 °C)

Pressure range 0 to 30 Psig (0 to 2 bar)

pH range 2 to 12

Sensor drift Less than 0.005 pH drift per day in laboratory

Initial one-point standardization accuracy using
internal sensor buffer

Shelf life 2 years at 77 °F (25 °C), expiration date on

Measuring method

Potentiometry pH glass electrode and double-junction

Wetted materials

Body material ULTEM™/PEI (USP Class VI)

pH electrode Proprietary glass formulation

conditions

Less than ±0.1 pH at pH 7

sensor label

reference electrode

O-ring EPDM (USP Class VI)

Operations parameters

Process connection 1-in. barb fitting

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Table 1-1: Rosemount™ 550pH Sensor Specifications (continued)

Specifications Materials and units

Electrical connector Variopol (VP 8.0)

Sensitivity Greater than 57 mV / pH at 77 °F (25 °C)

Electrolyte Phosphate based (see SDS on product website)

Integral temperature sensor Pt-100

Gamma irradiation Up to 50 kGy

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1.3 Dimensional drawings

See Figure 1-1 for sensor dimensions

Figure 1-1: Rosemount 550pH A1M22E0VP000 Dimensional Drawing

A. VP8 cable connection

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8 Rosemount 550pH

Reference Manual Install the sensor

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2 Install the sensor

The sensor will be integrated into a 1-in. barb fitting, sealed by several zip tie connectors.

Note

Complete Step 1 through Step 3 before filling the single-use bioreactor with solution.

Procedure

1. Orient the sensor within 10 and 45 ° of horizontal.
See Figure 2-1.

Figure 2-1: Sensor Orientation

2. Wire the sensor to the transmitter and wait for the reading to stabilize.
See Wire the sensor for instructions on wiring the sensor to Rosemount

transmitters.

3. Use the Calibration menu on the transmitter to go to Slope/offset setting.

Recommendation: Do not fill the bag with buffer or other solution until after 3.b to
confirm sensor functionality.

a) Input the factory calibrated slope on the sensor label into the "Slope at 25 C"

field and press ENTER. Press EXIT to go back to the Calibration menu.

Do not touch the value in the "Offset" field. The offset will change and be
shown after 3.b.

Note

To find the sensor slope and cal pH, scan the two-dimensional barcode on
the sensor label.

See Figure 2-2.

™

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Figure 2-2: Enter Data on Calibrate Screen

b) In the Calibration menu, go to the "Standardize (grab)" option. Press ENTER

at the installation prompt since the reading should already be stabilized.
Input the Cal pH on the sensor label into the "pH of grab sample" field to
complete the initial sensor standardization.

Note

Use pH sensor prior to expiration date.

See Figure 2-2.

4. Clip the zip tie, locking the clip in place. Remove the circular warning tag if it is still
present.

See Figure 2-3.

Figure 2-3: Clipped Zip Tie

5. Press the slide lock into the unlocked position.
The small lip of the lock should be the side that is pressed in. See Figure 2-4.

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Reference Manual Install the sensor

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Figure 2-4: Unlocked Slide Lock

Note

Before continuing to the next step, ensure the sensor is fully submerged in the
solution.

6. Use the plunger assembly to push the sensor into the activated position.
See Figure 2-5.

Figure 2-5: Activated Sensor

7. Press the slide lock into the locked position.
The small tab on the slide lock should be protruding out. See Figure 2-6.

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Figure 2-6: Locked Slide Lock

8. Install a zip tie into the slide lock to prevent the sensor from returning to storage
position.

See Figure 2-7.

Figure 2-7: Zip Tie

Postrequisites

After the start-up is complete, there is no additional work needed on the sensor unless a
one-point standardization is deemed necessary. See One-point standardization based on a

grab sample for this procedure using an off-line sample.

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Reference Manual Wire the sensor

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3 Wire the sensor

Use the following wiring diagrams to wire the Rosemount™ 550pH Sensor to the
Rosemount 56, 1056, 1057, 1066, and 5081 Transmitters.

1. See Figure 3-1 to wire the Rosemount 550pH Sensor to the Rosemount 56, 1056, or
1057 Transmitter.

Figure 3-1: Wire the Rosemount 550pH Sensor to the Rosemount 56, 1056, or
1057 Transmitter

2. See Figure 3-2 to wire the Rosemount 550pH Sensor to the Rosemount 1066
Transmitter.

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Figure 3-2: Wire the Rosemount 550pH Sensor to the Rosemount 1066
Transmitter

3. See Figure 3-3 to wire the Rosemount 550pH Sensor to the Rosemount 5081
Transmitter.

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Figure 3-3: Wire the Rosemount 550pH Sensor to the Rosemount 5081
Transmitter

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Reference Manual Calibrate

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4 Calibrate

4.1 One-point standardization based on a grab sample

Complete the following steps to perform a one-point calibration against an off-line
sample. This procedure is written for a Rosemount ™ 56 transmitter.

Procedure

1. Press ENTER on the transmitter.

2. Select "S1 Measurement" or "S2 Measurement", depending on which sensor you
wish to standardize.

3. Select "Standardize (grab)" in the Calibration menu.

4. Once the pH reading of the sensor is stabilized, press ENTER to pass the installation
prompt.

5. Input the pH of the grab sample into the "pH of grab sample" field and press ENTER.

6. Press EXIT to complete the calibration.

The pH reading on the screen should now equal the pH of the grab sample.

Note

pH is a temperature dependent attribute. Please ensure off-line sample is at the same
temperature as the process, so that no errors in standardization occur.

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Calibrate Reference Manual

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Reference Manual Troubleshoot

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5 Troubleshoot

5.1 Faulty reading

The one-point standardization at startup has a faulty reading.

Recommended actions

1. Rotate sensor within sensor sleeve 90 degrees.

2. Repeat sensor standardization procedure.

5.2 Label information too small

The serial number, Cal pH, or slope are too small to read.

Recommended action

Scan the two-dimensional bar code on the sensor label to retrieve information.

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Troubleshoot Reference Manual

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20 Rosemount 550pH

Reference Manual China RoHS table

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6 China RoHS table

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China RoHS table Reference Manual

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22 Rosemount 550pH

Reference Manual EU Declaration of Conformity

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7 EU Declaration of Conformity

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EU Declaration of Conformity Reference Manual

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Reference Manual

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Reference Manual 25

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Rev. AA

2019

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