Electron Microscopy Sciences EMS 5000 User Manual
EMS 5000 Tissue Slicer
QUALITY INSPECTION SUMMARY
We have made every effort to manufacture this instrument to the highest quality standards. All assemblies
have been thoroughly tested and inspected at the factory as follows:
Initial Assembly Inspection ________
Initial QC Inspection/Calibration ________
24 Hour Burn-In ________
Packaging Inspection Initials: __________ Date: __________
Items included with any catalog number may be labeled and packaged separately in shipping carton.
EMS, Inc. maintains a system of traceability to allow for product notifications in the event that issues arise pertaining to the
recall or failure of critical components contained in this instrument. In order that we may properly notify you, we ask you to
complete the following information and return to:
Quality Assurance Department
Electron Microscopy Sciences
1560 Industry Road
Hatfield Pa 19440
Serial Number(s) __________ __________ __________
Installation and Functional Checkout Complete per section 2.2 and 2.4 of this manual:
Final Performance Inspection ________
Description Quantity Checked
85-00-1 EMS5000
Containing:
EMS5000 ________
Manual ________
Footswitch ________
Tray ________
Pedestal ________
Specimen Vise ________
Mounting Blocks ________
Magnifier ________
Stainless Steel Slicer Blades ________
1/16” hex Driver ________
55-AUS Australia ________
55-CH China ________
55-DAN Denmark ________
55-EURO Europe ________
55-ISR Israel ________
55-ITA Italy ________
55-JA Japan ________
55-SAF South Africa ________
55-SWI Switzerland ________
55-UK United Kingdom ________
55-USA North America ________
Accepted by: _____________________
Institution: _______________________
Date: ___________________________
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TABLE OF CONTENTS
EMS 5000 Tissue Slicer
0 Declarations
0.1 Declaration Of Conformity
0.2 Conditions For Use
1 Operational Manual
1.1 Features
1.2 Description
1.3 Operating Environment
1.4 Inventory
1.4.1 Items Described In This Manual
1.4.2 Additional Items Required For Operation
1.4.3 Replacement Items
1.4.4 Optional Accessories
1.5 Concepts
1.5.1 Design Description
1.6 Technical Summary
1.6.1 Specifications
1.6.2 Controls / Connectors
1.6.3 Compatibilities
1.7 Illustrative Procedure
2 Reference Manual
2.1 Reference Information
2.1.1 Packaging
2.1.2 Mounting
2.1.3 Inspection
2.1.4 Power Connections
2.1.5 Warranty
2.1.6 Policies
2.1.7 Service
2.2 Installation
2.3 Functional Checkout
2.4 Operational Information
2.5 Scheduled Maintenance
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We: Electron Microscopy Sciences
of: P.O. Box 550
Hatfield Pa 19440
declare that:
Equipment: Oscillating Tissue Slicer
Model: EMS 5000
Serial Number: ___________
in accordance with the following Directives:
73/23/EEC Low Voltage Directive
and its amending directives
89/336/EEC Electromagnetic Compatibility Directive
and its amending directives
98/37/EC Machinery Directive
and its amending directives
has been designed and manufactured to the following specifications:
EN61010-2001: Safety Requirements for electrical equipment for measurement,
control, and laboratory use: Part 1 - General Requirements
EN50081-1:1992 Electromagnetic Compatibility – Generic emission standard:
Part 1 - Residential, commercial, and light industry
EN292-1: 1991 Safety of machinery – Basic concepts, general principles for design
Part 1: Basic terminology, methodology
EN292-2/A1:1995 Safety of machinery – Basic concepts, general principles for design
Part 2: Technical principles and specifications
I hereby declare that the equipment named above has been designed to comply with the
relevant sections of the above referenced specifications. The unit complies with all essential
requirements of the Directives.
Signed by: __________________________________Date: ____________________
Name: Stacie Kirsch
Position: President
Done at: 1560 Industry Road Hatfiled, Pa 19440
Phone: 215-412-8400, Fax: 215-412-8450
E-mail:sgkcck@aol.com Website: http://www.emsdiasum.com
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0.2 CONDITIONS FOR USE
Intended Use
The EMS 5000 is intended for acquiring samples for: electrophysiology of fresh tissue,
immunocytochemistry and immunohistochemistry, histology, cytology, botanical research, and polymer
science.
Warnings
The EMS 5000 should not be disassembled beyond the indications of this manual. Any disassembly
beyond this may affect function and calibration. Do not use phenols or ketones to clean the tray, as it
will crack. Care should be taken when handling any blade. Do not contact the front edge of the blade.
The EMS5000 should not be placed in the vicinity of high sources of electromagnetic phenomena. If
repair is required please contact EMS at (215) 412-8400 for evaluation and to secure a return
authorization number if necessary.
Storage Precautions
Store at normal room temperatures between -34°C (-29°F) and 57°C (135°F). Do not expose to
temperatures below -39°C (-29°F) or greater than 70°C (158°F), or a relative humidity of less than 10%
or more than 100%, including condensation, or an atmospheric pressure less than 500hPa or greater
than 1060hPa for long-term storage.
Sterilization
Except for the pedestal and mounting blocks, the EMS 5000 components are not designed for
sterilization. (For questions on sterilization of the tray components, please contact the EMS Technical
Services department at (215-412-8400.) Any attempt to sterilize the non-tray components may result in
malfunction or component failure.
Handling
While a high degree of durability has been designed into the EMS 5000, care should be taken not to
drop the components. Do not force the tray on the platform, or the pedestal/vise in the tray. Place all
cables and leads where they will not be inadvertently pulled or tangled.
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1 OPERATIONAL MANUAL
1.1 FEATURES
New slicer head design minimizes vibration that causes tissue distortion.
User defined "travel window" reduces blade travel time between sections.
User selectable multiple slice mode for "hands-free" preparation of many slices.
Consistent live tissue slices of 10 microns achieved.
Membrane switch keypad, separate from module, isolates circuitry from solutions, reduces
“handedness”, and improves ergonomics.
Slice thickness up to 999 microns, set in 1 micron increments.
Focused LED's mounted to blade arm for constant, direct, cold-source illumination.
Two specimen mounting options: XY adjustable pedestal or vise/block arrangement.
Removable, easy to clean tray.
Accepts Razor, Glass, Diamond, and Sapphire blades.
Precise bar graph displays of blade oscillation and advance speeds.
Large LED display of slice counter/slice thickness.
Removable X2 magnifier lens swings away when not in use.
Removable slicer head for cleaning and maintenance.
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1.2 DESCRIPTION
The EMS 5000 is used for sectioning slice samples for use in the fields of electrophysiology of fresh
tissue, immunocytochemistry and immunohistochemistry, histology, cytology, botanical research, and
polymer science.
The new design of the slicer head minimizes Z-axis vibration, which dramatically improves slice surface
integrity. This improvement is especially useful for fresh tissue where IR/DIC imaging methods are used.
To reduce the blade travel time between slices, the EMS 5000 features a user-programmable travel
window. This allows the user to set the range of blade travel customized to the specimen sample,
reducing the time between slices. Once the range is set, the START/STOP button is used to toggle the
sectioning sequence.
The EMS 5000 allows the user to section in two different modes. In manual slice mode, the user can
perform all movements of the blade arm using the keypad, or a combination of the keypad and
footswitch. In multiple slice mode, the EMS 5000 will automatically perform repeatable sequences.
The tray is made of molded clear polycarbonate for improved viewing of the slice operation. The tray is
removable to facilitate specimen mounting and cleaning. Two focused LED’s are installed in the blade
head, which provide constant cold illumination of the specimen during the slicing process.
Two specimen mounting options are available to the user; a pedestal with forward/backward and side to
side tilting adjustments, and a vise and mounting block (2 sizes provided) assembly. The blocks are
used to quickly interchange multiple specimens on individual blocks.
The front panel of the EMS 5000 has precise bar graph displays of the blade oscillation and advance
speeds. A large, three digit display shows the number of slices performed, and the current slice
thickness setting. (The display toggles when either of the thickness keypad buttons are pressed once.)
The modular configuration of the keypad allows for ambidextrous and ergonomic operation. The keypad
incorporates membrane switch technology for durability and reliability in the laboratory environment.
The blade head is removable for occasional cleaning and maintenance. The blade clamp is designed to
accept razor, sapphire, diamond, and glass blades.
A separate self-contained refrigeration system will be made available soon as an option.
1.3 OPERATING ENVIRONMENT
The EMS 5000 has been designed to operate in a typical laboratory setup. It should be placed on a
solid, flat surface that is level and free from contaminants and vibration. The EMS 5000 should not be
placed or used near or during high sources of electromagnetic phenomena.
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