Edu Science M800X User Manual
LABORATORY MICROSCOPE
MICROSCOPE DE LABORATOIRE
MICROSCOPIO DE LABORATORIO
800
#5F60948
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M800x Microscope Set
Supervision by Adults
Read and follow the instructions, safety
rules and rst aid information.
This Microscope set is intended for
children over the age of 8 years. Children
should only use this device under
adult supervision. Never leave a child
unsupervised with this device.
Accessories in this experimental kit may
have sharp edges and tips. Please store
the device and all of its accessories and
aids out of the reach of young children
when not being used due to a risk of
INJURY.
This device contains electronic
components that are powered by
batteries. Batteries should be kept out of
children’s reach. When inserting batteries
please ensure the polarity is correct.
Insert the batteries according to the
displayed +/- information.
Fire/Danger of Explosion!
Do not expose the device to high
temperatures. Use only battery types
recommended. Never mix old and new
batteries (replace all batteries at the
same time). Never mix Alkaline, standard
(Carbon Zinc) or rechargeable batteries.
Never short circuit the device or batteries
or throw into a re. Exposure to high
temperatures or misuse of the device
can lead to short circuits, re or even
explosion! Leaking or damaged batteries
can cause injury if they come into contact
with the skin. If you need to handle such
batteries please wear suitable safety
gloves.
Chemicals
Any chemicals or liquids used in
preparing, using, or cleaning should
be kept out of reach of children. Do not
drink any chemicals! Hands should be
washed thoroughly under running water
after use. In case of accidental contact
with the eyes or mouth rinse with water.
Seek medical treatment for ailments
arising from contact with the chemical
substances and take the chemicals with
you to the doctor for treatment.
RISK of material damage
Never take the device apart. Please
contact our service center and send the
device in for repair as needed.
Do not subject the device to temperatures
exceeding 140˚F.
TIPS on cleaning
Remove batteries from device before
cleaning.
Microscope Care
Clean the exterior of device with a dry
cloth. Do not use cleaning uids so as
to avoid causing damage to electronic
components. Clean the lens (objective
and eyepiece) only with a soft lint-free
cloth (e.g., micro ber). Do not use
excessive pressure - this may scratch
the lens. Protect the device from dust and
moisture. Store the device in its original
packaging. Batteries should be removed
from the device if not used for a long
period of time.
DISPOSAL
Keep packaging materials (plastic bags,
rubber bands, etc.) away from children.
There is a risk of SUFFOCATION.
Dispose of the packaging materials
as legally required. Consult the local
authority on the matter if necessary.
DISPOSAL
Dispose of the packaging materials
properly, according to their type, such as
paper or cardboard. Contact your local
waste-disposal service or environmental
authority for information on the proper
disposal.
Please take the current legal regulations
into account when disposing of your
device. You can get more information on
the proper disposal from your local
waste-disposal service or environmental
authority.
Contents:
• Microscope
• Slide case
• 5 Prepared slides
• 18 Blank slides
• 18 Slide covers
• 18 Labels
• 4 Collection vials
• Red-dye vial
• Green-dye vial
• Pipette
• Tweezers
• Magnifying glass
• Graduated cylinder
• Spatula
• Shrimp hatchery
• Shrimp eggs
• Carrying case
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M800x Microscope Set
The Parts of Your Microscope:
1 (2) Exchangeable Wide Field
Eyepiece (WF10x, WF20x)
2 Soft Rubber Eye Cups
3 Rotating Eyepiece Head
4 Coarse and Fine Focus Knob
5 Stage
6 Metal Stage Clips
7 Color Filter Wheel
8 Objective Turret
9 4x, 10x 40x Objectives
10 LED Lower Illumination
11 LED Upper Illumination
12 Base with Battery Compartment
13 3 Position Illumination Switch
14 Microscope Arm
15 Carrying Case
Additional Contents:
16 (5) Prepared Glass Slides
17 (18) Blank Glass Slides
18 (18) Slide Covers
19 (18) Labels
20 (4) Collection Vials
21 Red-Dye Vial
22 Green-Dye Vial
23 Pipette
24 Tweezers
25 Magnifying Glass
26 Graduated Cylinder
27 Spatula
28 Shrimp Hatchery
29 Shrimp Eggs
Congratulations! You’ve chosen one
of the highest quality microscopes
available for young explorers. Read
the following instructions carefully
to get the greatest bene t from your
precision instrument. Then try out the
experiments to begin your investigation
of the fascinating world around you.
How do I use my microscope?
Before you use your microscope, make
sure that the table, desk or whatever
surface that you want to place it on is
stable, and is not subject to vibration. If
the microscope does need to be moved,
use the arm and base for support while
carefully transferring it.
Install three “AA” batteries (not included)
in the battery compartment on the
bottom of the microscope. Open battery
door on the bottom of the microscope
and insert the batteries according to the
displayed +/- information. Snap-close
the battery compartment door.
Once the microscope is in a suitable
location and batteries installed, check
the light sources to make sure that
they both illuminate by toggling the
light switch (Fig. 13) to the ALL position
(indicated by the I,ʽʽ0ʼʼ, and II). Use a
cleaning cloth (e.g., micro ber) to gently
wipe the lenses off. If the stage (Fig. 5)
is dirty with dust or oil, carefully clean
it off.
The stage is raised and lowered only by
using the focus adjustment knob (Fig 4).
How do I operate the LED illumination?
This microscope is equipped with
two modern LED lights (light-emitting
diodes) that illuminate the specimen
from the top and below the stage
(Fig. 5) You can use different lighting
techniques to illuminate objects and
specimens from opaque to transparent.
Locate the light switch (Fig. 13) on the
base of the microscope. Toggle the
switch to the rst position (indicated
by the I), and the lower LED light (Fig.
10) will illuminate. Move the Toggle to
the second position (indicated by the
0) to turn off all illumination. Move the
toggle to the nal position (indicated by
II), and both LED lights (Fig. 10 & 11)
will illuminate.
The color lters wheel (Fig. 7) is
located below the microscope stage
(Fig. 5). Filter wheels help you observe
very bright or clear specimens. Using
these lters (blue, yellow, red and
clear), you can choose from various
colors. The lters wheel also has
four different size apertures so you
can adjust the brightness levels on
objects / specimens. Filter wheels help
you better recognize components of
colorless or transparent objects (e.g.,
grains of starch, protozoa). Rotating the
lter wheel in combination with toggling
the lower light or both lights on/off will
allow you to view the object / specimen
and achieve the desired effect.
How do I adjust my microscope
correctly?
Place on a suitable location as
described above and sit in a
comfortable viewing position. This
microscope includes a rotating head
(Fig. 3), which allows for easy viewing
in multiple positions as well as sharing
with others the amazing images you
have discovered with your microscope.
Always start each observation with
the lowest magni cation. Adjust the
microscope stage (Fig. 5) so that the
stage is in the lowest position. Turn
the objective turret (Fig. 8) until it clicks
into place at the lowest magni cation
(Objective 4x). Note: Before you
change the objective setting, always
move the microscope stage (Fig. 5)
to its lowest position by rotating the
focus knob (Fig. 4). Lowering the stage
by rotating the focus knob will avoid
causing any damage to the specimen
slide or microscope. When starting an
observation always start with the WF
10X eyepiece (Fig. 1) in the rotating
head (Fig. 3).
Quick Fact - The highest magnifi cation
is not always the best for every specimen!
Exchangeable WF Eyepieces
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24
25
26
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27
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M800x Microscope Set
How do I observe the specimen?
Sitting in your location with adequate
illumination chosen from the color
lter wheel, the following basic rules
should be observed: Start with a simple
observation at the lowest magnication.
Position the object or specimen in the
middle of the stage under the stage
clips (Fig. 6), centered over the lower
LED light (Fig. 10). Focus the image by
rotating the focus knob (Fig. 4) until a
clear image appears in the eyepiece.
NOTE: The higher the magnication,
the more light you will require for a
good image quality.
Quick Fact - The item you want to
observe with the microscope is known
as the object or specimen.
Place the prepared slide directly under
the objective on the microscope stage
(Fig. 5) securing with the stage clips
(Fig 6). The prepared slide should
be located directly over the lower
illumination (Fig. 10). Look through the
eyepiece and carefully turn the focus
knob (Fig. 4) until the image appears
clear and sharp. Now you can select
a higher magnication by changing
the WF eyepiece to the 20X (Fig. 1).
When the WF 20X lens is inserted
in the barrel of the rotating head, the
magnication is doubled. Higher levels
of magnication can be achieved by
turning the objective turret (Fig. 8) to
a higher setting (10x or 40x). For best
results, return the WF 10x eyepiece
to the lowest power of magnication
before changing the power on the
turret. Replacing the WF 10x eyepiece
upon every rotation of the turret allows
for easier transitions in magnication.
Following this procedure creates
a steady increase of magnication
without overpowering the view of the
object. The following magnications
should be considered: 40x, 80x, 100x,
200x, 400x, then 800x.
Each time the magnication changes
(due to an eyepiece or objective change),
the image sharpness must be readjusted
with the focus knob (Fig. 4). When doing
this, be careful because if you move
the microscope stage too quickly, the
objective and the slide could come into
contact and cause damage to the slide
or microscope.
For transparent objects (e.g., protozoa),
light is projected by the lower LED light,
traveling from below the stage, through
the objective and eyepiece, and nally
into your eye. This process of light
transmission is known as microscopy.
Many microorganisms found in water,
plant components, and the smallest
animal parts are transparent in nature.
Opaque specimens, on the other hand,
will need to be prepared for viewing.
Opaque specimens can be made
transparent by a process of treatment
and penetration with the correct
materials (media), or by slicing. You can
read more about creating specimens in
the following experiment sections.
Cleaning Tips
Ensure your microscope has a long
service life. Clean the lens (objective
and eyepiece) only with a soft lint-free
cloth (e.g., microber). Do not press
hard as this might scratch the lens. Ask
your parents to help if your microscope
is really dirty. The cleaning cloth should
be moistened with cleaning uid
and the lens wiped clean using little
pressure. Make sure your microscope
is always protected against dust and
dirt. After use, leave it in a warm room
to dry, then return it to the carrying case
provided.
This microscope can be the gateway
to a fun, creative, learning process
and will open the door to advanced
knowledge of the world around you.
Allowing you to explore the various
elds of science from Biology to Botany
to Chemistry and beyond, so have fun
exploring the exciting world of science.
Experiment Instructions
WARNING!
• Keep chemicals and corrosive liquids
out of the reach of children!
• Do not Ingest any chemicals!
• Wash your hands with soap thoroughly
under running water after use!
Introduction
Here are a few tips about how to take
a better look at the wonderful world
of microorganisms and crystals. For
example, you’ll learn how to prepare
your object / specimen so that you
can look at it with the microscope.
The numerous experiments described
should make you curious and want to
use your microscope more.
What Kind of Objects?
With a magnifying glass, you can
look at non-transparent (i.e. opaque)
objects, for example, small animals,
parts of plants, tissues, etc. Here,
the light falls onto the object and is
reected back through the magnifying
lens and into your eye. With your
microscope, however, you can also
observe transparent objects, in which
the light from the lamp goes through the
opening on the stage and the prepared
specimen. Then, it passes through the
objective, the body of the microscope
and through the eyepiece into the eye.
Many microorganisms in water, parts of
plants and the tiniest animal parts are
naturally transparent. For other things,
you must make them transparent
through a treatment or penetration with
the right materials (media), or by taking
the thinnest slices from them using
your hand or a specimen slicer (not
included) to be able to examine them
with your microscope. You’ll now nd
out how this is done.
How to Produce Thin Specimen Slices
WARNING:
Only do this with an adult’s supervision!
Ask your parents to help you! As already
mentioned, you need to get the thinnest
slices possible from an object so that
they are transparent and can be looked
at under the microscope. First, you’ll
need a simple candle. Place the wax
from the candle in an old pot and heat
it on the stovetop until it becomes liquid.
Now, use tweezers (Fig. 24) to dip the
object in the liquid wax a few times.
Attention: The wax is very hot! Be
careful. After each dip, allow the wax to
harden and then dip the object into the
wax again. When the wax around the
object has hardened completely, you
can use a specimen slicer to cut thin
slices from it. These slices are to be laid
on a slide and covered with a cover slip
or slide cover (Fig. 18).
The Production of Specimens
There are two basic types of specimens:
Permanent specimens and short-term
specimens.
Short-term Specimens
Short-term specimens are produced
from objects that you want to look at,
but don’t want to keep in your specimen
collection. These specimens are only
meant to be observed for a short period
of time, after which they are disposed
of. For short-term specimens, place the
object on the slide and place a cover
slip on top of it. After looking at the
object, clean the slide and the cover
slip. One of the secrets of successful
observation with your microscope is
the use of clean slides and cover slips.
Spots or stains would only distract you
when looking at an object.
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No recognizable image
No Image
No Light
Turn on light
Readjust focus
Start with the
Lowest power objective (4X)
Lowest power Eyepiece (10X)
Center object on slide under
Start with the
Lowest power objective (4X)
Lowest power Eyepiece (10X)
Replace batteries
Check on/o position
Troubleshooting Table
Problem Solution
M800x Microscope Set
Permanent Prepared Specimens
Permanent specimens are those
produced from objects that you would
like to look at again and again. The
preparation of dry objects (pollen, the
wings of a y, etc.) can only be done
with special glue. You’ll nd such glue
at a local hobby store, identied as
“gum media.” Objects that contain liquid
must rst have the liquid taken out of
them.
How to Prepare a Dry Object
First, place the object in the middle of a
clean slide and cover it with a drop of glue
(gum media). Then place a cover slip on
the object. Lightly press the cover slip, so
that the glue spreads to the edges. Then
let the specimen harden for 2-3 days.
When the specimen is rmly glued, you
will be able to use it.
How to Prepare Smear Specimen
For a smear specimen, a drop of the
liquid to be observed (e.g., water from
a puddle in the forest) is placed on the
end of the slide using a pipette. Then
you can smear the liquid across the
slide with the help of a second slide.
Before observing, let the substance dry
together for a few minutes.
Experiments
Experiment No. 1:
Black and White Print
Objects:
1. A small piece of paper from a
newspaper with a black and white
picture and some text.
2. A similar piece of paper from a
magazine.
In order to observe the letters and the
pictures, produce a short-term slide from
each object. Now, set your microscope
to the lowest magnication and use the
specimen from the newspaper. The
letters on the newspaper look frayed
and broken, since they are printed
on raw, low-quality paper. The letters
on the magazine look smoother and
more complete. The pictures in the
newspaper are made up of many tiny
dots, which appear slightly smudgy.
The halftone dots of the magazine
picture are clearly dened.
Experiment No. 2:
Color Print
Objects:
1. A small piece of color printed
newspaper.
2. A similar piece of paper from a
magazine.
Make short-term specimens from the
objects and observe them with the lowest
magnication. The colored halftone
dots of the newspaper often overlap.
Sometimes, you‘ll even notice two colors
in one dot. In the magazine, the dots
appear clear and rich in contrast. Look at
the different sizes of the dots.
Experiment No. 3:
Textile Fibers
Objects and accessories:
1. Threads from various fabrics (e.g.,
cotton, linen, wool, silk, rayon, nylon,
etc.).
2. Two needles.
Each thread is placed on a slide and
frayed with the help of the two needles.
Next, wet the threads and cover them
with a cover slip. Set the microscope
to one of the lower magnications.
Cotton bers come from a plant, and
look like a at, twisted ribbon under
the microscope. The bers are thicker
and rounder at the edges than in the
middle. Cotton bers are basically long,
collapsed tubes. Linen bers also come
from a plant, and they are round and
run in one direction. The bers shine
like silk and exhibit countless bulges on
the thread. Silk comes from an animal
and is made up of solid bers that are
small in diameter, in contrast to the
hollow plant-based bers. Each ber is
smooth and even and looks like a tiny
glass tube. The bers of the wool also
come from an animal. The surface is
made of overlapping sleeves that look
broken and wavy. If possible, compare
wool from different weaving mills. In
doing so, take a look at the different
appearance of the bers. Experts can
determine which country the wool
came from by doing this. Rayon is a
synthetic material that is produced by
a long chemical process. All the bers
have solid, dark lines on the smooth,
shiny surface. After drying, the bers
curl into the same position. Observe the
differences and the similarities.
Experiment No. 4:
Table Salt
Object: Common table salt.
First, place a few grains of salt on a
slide and observe the salt crystals with
the lowest setting of your microscope.
The crystals are tiny cubes and are all
the same shape.
Experiment No. 5:
Production of Salt Crystals
Objects and accessories:
1. Table salt.
2. A graduated cylinder lled halfway
with warm water to dissolve the salt.
3. Cotton thread.
4. Paper clips.
5. A matchstick or pencil.
Add salt to the water until it no longer
dissolves. You now have a saturated
salt solution. Wait until the water has
cooled. Fix a paper clip to the end
of the cotton thread. The paper clip
serves as a weight. Tie the other end
of the cotton thread into a knot around
the match, and dip the end with the
paper clip in the salt solution. Place
the match horizontally on top of the test
tube. It prevents the cotton thread from
slipping all the way down into the test
tube. Now, place the tube in a warm
place for 3-4 days. If you take a look
at the glass after a few days under the
microscope, you can see that a little
colony of salt crystals has formed on
the cotton thread.
Experiment No. 6:
How do you raise Brine Shrimp?
Accessories (from your microscope set):
1. Shrimp eggs.
2. Sea salt.
3. Hatchery.
4. Yeast. (not included)
Brine Shrimp, or “Artemia Salina” as
they are called by scientists, have
an unusual and interesting life cycle.
The eggs produced by the female are
hatched without ever being fertilized by
a male shrimp. The shrimp that hatch
from these eggs are all females. In
unusual circumstances (e.g., when the
marsh dries up), the male shrimp can
hatch. These males fertilize the eggs
of the females and from this mating,
special eggs are produced. These
eggs, called “winter eggs,” have a
thick shell, which protects them. The
winter eggs are very resistant and
capable of survival, even if the marsh
or lake dries out, killing off the entire
shrimp population. The winter eggs can
exist for 5-10 years in a “sleep” status
and will only hatch when the proper
environmental conditions occur. These
are the type of eggs you have in your
microscope set.
The Incubation of the Brine Shrimp
In order to incubate the shrimp, you
rst need to create a salt solution that
corresponds to the living conditions of
the shrimp. For this, put a half liter of
rain or tap water in a container. Let the
water sit for approx. 30 hours. Since
the water evaporates over time, it is
advisable to ll a second container with
water and let it sit for 36 hours. After the
water has sat stagnant for this period
of time, add half of the included sea
salt to the container and stir it until all
of the salt is dissolved. Now, put a few
eggs in the container and cover it with
a dish. Place the glass container in a
bright location, but don‘t put it in direct
sunlight. Since you have a hatchery,
you can also add the salt solution
along with a few eggs to each of the
four compartments of the tank. The
temperature should be around 77º F
(25ºC). At this temperature, the shrimp
will hatch in about 2-3 days. If the water
in the glass evaporates, add some
water from the second container.
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