USER’S
GUIDE
MICROPIPETTE
BIOTIX COBRA PIPETTE
Contents Page
1- INTRODUCTION 3
2- PARTS CHECK LIST 3
3- DESCRIPTION 4
4- SETTING THE VOLUME 6
5- USER ADJUSTMENT 7
6- PIPETTING 8
7- GENERAL GUIDELINES FOR GOOD PIPETTING 10
8- ACCESSORIES 12
9- GLP FEATURES 12
10- TROUBLESHOOTING 13
11- LEAK TEST 14
12- MAINTENANCE 15
13- CLEANING AND STERILIZATION 20
14- SPECIFICATIONS 24
15- SPARE PARTS 25
16- MAINTENANCE & CALIBRATION 30
17- WARRANTY 31
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USER’S
USER’S
GUIDE
GUIDE
1 - INTRODUCTION
The LabPRO pipette is an air displacement mechanism
and is used with disposable pipette tips.
This pipette line provides:
• Light and comfortable body
• Low pipetting forces ensuring ergonomics and users’
well-being
• Unique patented Lock/Unlock mecanism for results
you can trust
Eight single channel models cover a volume range from
0.2 µL to 10 mL.
Eight multichannel models (4 x 8-ch and 4 x 12-ch)
cover a volume range from 0.5 µL to 300 µL.
2 - PARTS CHECK LIST
Just take a moment to verify that the following items
are present:
Single models
• LabPRO Pipette,
• User’s Guide,
• Certificate of conformity,
• Calibration Key.
• Tip-ejector extention for 10µl only
Multichannel models
• LabPRO Pipette,
• User’s Guide,
• Ejector spacer for short collar tips (only for multi-
channels models 10 µL),
• Certificate of conformity,
• Calibration key.
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BIOTIX COBRA PIPETTE
VWR® MECHANICAL PIPETTE
3 - DESCRIPTION
Single models
Multichannel models
Color-coded push-button
Lock/Unlock mecha-
nism & Tip-ejector
button
Handle
Counter
Connecting nut
Tip-holder
Tip-ejector
Ejector clip
Ejector support
Ejector lock
Cover
Tip-holders
FIGURE 1
For 2µl and 10µl Single channel
models, a tip-ejector extention
is supplied to fit with short tips
models.
To fit the tip-ejector extention:
Slide the extention over the
tip-holder
Push the extention firmly
onto the end of the tip-ejector until it clicks into place
4
FIGURE 2
USER’S
USER’S
GUIDE
GUIDE
To remove the tip-ejector extention:
Gently twist the extention
Pull it away from the pipette
Ejector spacer for Multichannel models (X10µl only)
Multichannel models fit with long collar tips. If you use
short collar tips, you might have to insert the ejector
spacer especially indicated for that:
• Remove the tip-ejector, keep both ejector locks depressed; pull the tip-ejector down.
• Insert the broad ejector spacer and click it to the tipejector.
•
To refit the tip-ejector, gently re-insert the tip-ejector
vertically into the rails of the ejector support.
Ejector spacer
FIGURE 3
Long collar tips
Short collar tips
4 - SETTING THE VOLUME
The volume of liquid to be aspirated is set using the
volumeter. The digits are colored either black or green
to indicate the position of the decimal point, depending
on the model (see examples below).
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BIOTIX COBRA PIPETTE
0
7
5
X10µl
7.5 µL
1
2
5
X20µl
12.5 µL
X200µl
1
2
5
125 µL
X300µl
1
2
5
125 µL
1
5
2µl
1.25 µL
2
0
7
5
10µl
7.5 µL
1
2
5
20µl
12.5 µL
100µl
0
7
5
75 µL
200µl
1
2
5
125 µL
1000µl
0
7
5
0.75 mL
5000µl
1
2
5
1.25 mL
10ml
0
7
5
7.5 mL
VWR® MECHANICAL PIPETTE
Single models Multichannel models
MODEL Color of volumeter digits
Black Green Increment
2µl µL 0.01 µL 0.002 µL
10µl to 20µl µL 0.1 µL 0.02 µL
8X-10µl, 8X-20µl
12X-10µl, 12X-20µl µL 0.1 µL 0.02 µL
100µl, 200µl, 8X-200µl, 12X-200µl µL - 0.2 µL
8X-300µl, 12X-300µl µL - 0.2 µL
1000µl 0.01 mL mL 0.002 mL
5000µl 0.01 mL mL 0.002 mL
10 mL mL 0.1 mL 0.02 mL
Lock system:
For trusted results, the volume selection is lockable.
First step, unlock the
tip-ejector button by
pushing it up
Second step, the
volume is set by turning
the push button
3 Third step, push down
the tip-ejector button.
The selected volume is locked.
6
Unlocked
Position
USER’S
USER’S
GUIDE
GUIDE
To obtain maximum accuracy when setting the volume,
proceed as follows:
> when decreasing the volume setting, slowly reach the
required setting, making sure not to overshoot the mark.
> when increasing the volume setting, pass the required
value by 1/3 of a turn and then slowly decrease to reach
the volume, making sure not to overshoot the mark.
> To adjust perfectly the last digit, it is even more precise
to do so on the Lock position.
5 - USER ADJUSTMENT
The calibration of the LabPRO Pipettes has been performed with distilled water and very high precision
volumetric instruments. Adjusting the pipette can be
necessary for different solutions due to their density,
viscosity, surface tension and/or vapor pressure etc.
Calibration is sometimes recommended when it is used
in high altitudes or with special tips. It can also be recalibrated when long pipette tips are used. Performance
testing should take place in a draught-free room at 1530° C, constant to ± 0.5° C and humidity above 50 %.
To adjust the volume setting:
Remove the push button.
Use the calibration key to take off the block cover.
a. Insert the metal rod into the
calibration tool on the hexagonal side.
b. Engage the two rectangular
FIGURE 5
Part 1
hooks of part 2 into the two
holes of the block cover. You
Part 2
should feel them clipped on
firmly in the hidden part of the
pipette (see figure 5).
c. Make sure to hold the part 1 at the top cap, along
the part 2.
d. Turn the part 2 counterclock-
FIGURE 6
wise slowly to remove the block
cover (see figure 6).
Put the block cover apart.
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BIOTIX COBRA PIPETTE
Use the calibration key to adjust
the pipette
a. Insert the metal rod into the
calibration key on the circle tip
side (see figure 7). Lock it into
place. You should feel the internal part of the calibration key
clamped and clipped on firmly.
If it is not the case, turn it counterclockwise slowly. Then, turn
the plastic connecting nut of the
part 1 slowly until it’s locked in
place (see figure 8).
b. Hold the connecting nut of the
part 1 with one hand
and turn
the part 2 with the other one according to the correction needed
(see figure 9).
Once the desired volume
is set, remove the calibration key and put back
the block cover using
the hexagonal side. Turn
it clockwise to lock the
block cover. Put back the
push button.
With reference water, one
revolution (1/8 turn of the
calibration tool) of the volume setting corresponds to:
Volume range Vol. per 1/8 turn
(equivalence in µL)
2µl 0.012
10µl 0.047
20µl 0.120
100µl 0.48
200µl 1.20
1000µl 4.75
5000µl 23.8
10ML 48
8X-10µl
12X-10µl
8X-20µl
12X-20µl
8X-200µl
12X-200µl
8X-300µl
12X-300µl
FIGURE 7
FIGURE 8
FIGURE 9
i.e: 1 full turn is 0.096
0.058
0.120
1.20
1.90
6 - PIPETTING
Fitting the tips
LabPRO pipettes have been designed to fit universal
tips.
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Pre-rinse the tips
Some liquids (e.g.protein-containing solutions and organic
solvents) can leave a film of liquid on the inside
wall of the tip; pre-rinse the tip to minimize any errors
that may be related to this phenomenon.
Pre-rinsing consists of aspirating the first volume of
liquid and then dispensing it to waste. Tips will not fall
off nor will they have to be manually positioned.
Make sure first that the pipette is calibrated with the
tips that you are using. Then, subsequent volumes that
you pipette will have levels of accuracy and precision
within specifications. Using other tips may require a
validation of the pipetting system.
1st stop
2nd stop
Figure 10
Aspirate
Press the push-button to the first stop (this corre-
sponds to the set volume of liquid).
Hold the pipette vertically and immerse the tip in the
liquid (see immersion depth table, page 10).
Release the push-button slowly and smoothly (to
top position) to aspirate the set volume of liquid.
Wait one second (time depends on model, see table
page 10); then withdraw the pipette tip from the
liquid.
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BIOTIX COBRA PIPETTE
You may wipe any drop lets away from the outside
of the tip using a medical wipe, however if you do so
take care to avoid touching the tip’s orifice.
/ For the multichannel models, use a reagent reservoir.
Dispense
Place the end of the tip against the inside wall of the
recipient vessel (at an angle of 10° to 40°).
Press the push-button slowly and smoothly to the
first stop.
Wait for at least a second, then press the push-button
to the second stop to expel any residual liquid from
the tip.
Keep the push-button pressed fully down and (while
removing the pipette) draw the tip along the inside
surface of the vessel.
Release the push-button, smoothly. Eject the tip by
pressing firmly on the tip-ejector button.
/ For the multichannel models, use a reagent reservoir.
7 - GENERAL GUIDELINES FOR GOOD
PIPETTING
Make sure that you operate the push-button slowly
and smoothly.
When aspirating, keep
the tip at a constant
depth below the surface of the liquid (refer to the table).
Change the tip before
aspirating a different
liquid, sample, or reagent.
Change the tip if a
droplet remains at the
end of the tip from the
previous pipetting operation.
10
Table Immersion Depth and Wait Time
Model Immersion Wait Time
Depth (mm) (seconds)
2µl 1 1
10µl 1 1
20µl 2-3 1
100µl 2-4 1
200µl 2-4 1
1000µl 2-4 2-3
5000µl 3-6 4-5
10mL 5-7 4-5
8X-10µl, 12X-10µl 1 1
8X-20µl, 12X-20µl 2-3 1
8X-200µl, 12X-200µl 2-3 1
8X-300µl, 12X-300µl 2-4 1
USER’S
USER’S
GUIDE
GUIDE
Each new tip should be pre-rinsed with the liquid to
be pipetted.
Liquid should never enter the tip-holder; to prevent
this:
•
press and release the push-button slowly and
smoothly,
• never turn the pipette upside down,
•
never lay the pipette on its side when there is liquid
in the tip.
If you use the same tip with a higher volume, pre-
rinse the tip.
For volatile solvents you should saturate the aircushion of your pipette by aspirating and dispensing
the solvent repeatedly before aspirating the sample.
When the pipetted liquid is not at room tempera-
ture, pre-rinse the tip several times before use.
You may remove the tip-ejector (see chapter 12 -
Maintenance) to aspirate from very narrow tubes.
After pipetting acids or other corrosive liquids that
emit
vapors, remove the tip-ejector, the tip-holder,
rinse, dry
and lubricate the piston (see chapter 12 -
Maintenance).
Do not pipette liquids having temperatures above
70°C or below 4°C. The pipette can be used between
+4°C and +40°C but the specifications may vary according to the temperature (refer to the ISO8655-2
standard for conditions of use).
/ Pipettes should be held in the vertical position.
8 - ACCESSORIES
To avoid the possibility of liquid running back into the
pipette, store the pipette vertically.
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BIOTIX COBRA PIPETTE
To identify or personalize your pipette, identification
clips are available:
Identification clips (mixed colors set of 10) FE07060
Identification clips (red, set of 10) FE07061
Identification clips (yellow, set of 10) FE07062
Identification clips (green, set of 10) FE07063
Identification clips (blue, set of 10) FE07064
Identification clips (white, set of 10) FE07065
FIGURE 12
9 - GLP FEATURES
The Serial Number is engraved
on the body of the pipette. It
provides a unique identification
of the pipette and the manufacturing date. Example: 15A1425
To know the specific details
about your pipette, see thetable.
The Bar Code on the box and the
certificate of conformity provide
traceability of your pipette.
Year /CODE Month/CODE NUMBER
(example)
2015/15 January/A 0001
2016/16 February/B 0325
2017/17 March/C 0500
2018/18 April/D 0750
2019/19 May/E 1000
2020/20 June/G 1300
2021/21 July/H 1600
2022/22 August/J 2000
2023/23 September/K 2400
2024/24 October/L 2600
2025/25 November/M 2800
2026/26 December/N 3000
10 - TROUBLESHOOTING
A quick inspection of the pipette may help you to detect
a problem.
The following tables may help you to identify and correct
the problem you might encounter.
For any other symptom or if you can’t solve the problem,
please contact your supplier.
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For single models
Symptom Possible Cause Action
Pipette is leaking sample Damaged tip-holder Replace the tip-holder
Worn O-ring or seal Replace both parts and lubricate
Pipette won’t aspirate Worn O-ring Replace both parts and lubricate
Damaged tip-holder Replace the tip-holder
Connecting nut is loose Tighten connecting nut
Damaged or corroded piston Return pipette to supplier
Improper repair or assembly See Chapter 12 - Maintenance
Pipette is inaccurate Improper repair or assembly See Chapter 12 - Maintenance
Unscrewed tip-holder Tighten connecting nut
Connecting nut is loose Tighten connecting nut
Pipette is not precise Tip-holder is loose Tighten connecting nut
Connecting nut is loose Tighten connecting nut
Incorrect operator technique Operator training
Damaged or corroded piston(s) Return pipette to supplier
Damaged tip-holder(s) Replace the tip-holder
Worn O-ring or seal Replace both parts and lubricate
Tips fall off or do not Low quality tips
fit correctly
Dirty tip-holder Clean the tip-holder with
isopropanol or ethanol
Damaged tip-holder(s) Replace the tip-holder
Damaged tip-ejector Replace tip-ejector
The tip-ejector is loose
The ejector lock is misaligned Align the ejector lock
Pipetting seize up Piston need lubricant Lubricate piston assembly
Use good quality tips to have opti
mum results of the pipetting system
Assemble the tip-ejector properly
-
For Multichannel models
Symptom Possible Cause Action
Tips fall off or Low quality tips
do not fit correctly
Tip-ejector damaged Replace tip-ejector
Ejector spacer damaged Replace ejector spacer
Dirty tip-holder Clean them with ethanol or
isopropanol
Damaged tip-holder Contact your local supplier
Pipette won’t aspirate Connecting nut is loose Tighten connecting nut
Pipette is inaccurate Connecting nut is loose Tighten connecting nut
Pipette is not precise Connecting nut is loose Tighten connecting nut
Incorrect operator technique Operator training
Use good quality tips to have opti
mum results of the pipetting system
-
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BIOTIX COBRA PIPETTE
11 - LEAK TEST
This test may be performed at any
time to check that the pipette does
not leak, especially after performing a maintenance or sterilization
procedure. If a pipette fails this
test, replace the O-ring and seal.
After making sure that the pipette
is correctly reassembled, repeat
this test.
For the 2µl to 200µl Single channel models:
Fit Pipette Tips.
Set the pipette to the maximum volume given in the
specifications, and pre-rinse.
Aspirate the set volume from a beaker of distilled
water.
Maintain the pipette in the vertical position and wait
for 20 seconds.
If a water droplet appears at the end of the tip there
is a leak.
If you see no droplet, re-immerse the tip below the
surface of water.
The water level inside the tip should remain constant;
if the level goes down there is a leak.
FIGURE 14
no leak
For the 1000µl, 5000µl and 10mL Single channel
models:
Fit Pipette Tips.
Set the pipette to the maximum volume given in the
specifications.
Aspirate the set volume from a beaker of distilled
water.
Maintain the pipette in the vertical position and wait
for 20 seconds.
If a water droplet appears at the end of the tip, there
is a leak.
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For the Multichannel models (8X - 12X):
Fit Pipette tips.
Set the pipette to the maximum volume given in the
specifications, and pre-rinse.
Aspirate the set volume from a reagent reservoir of
distilled water.
Maintain the pipette in the vertical position and wait
for 20 seconds; fluid level in tips should remain constant.
If a water droplet appears at the end of the tip, there
is a leak.
If you see no droplet, for volumes below 200 µL, re-
immerse the tip below the surface of water.
The water level inside the tip should remain constant;
if the level goes down there is a leak.
12A - MAINTENANCE FOR THE SINGLE
CHANNEL MODELS ONLY
Routine maintenance will help to keep your pipette in
good condition, ensuring a continued high level of performance.
Maintenance is limited to:
• Cleaning or sterilization (see Chapter 13 - Cleaning
and Sterilization).
• Replacing spare parts.
• Greasing the piston assembly.
Lubricant tube 1 g is available under the reference
FE07066
2µl and 10µl Single channel models should not be
disassembled, so you may only replace the push-
button, tip-ejector and its adapter.
With these pipettes if the tip-holder is damaged, the
piston may also be damaged.
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BIOTIX COBRA PIPETTE
Changing the tip-ejector
To remove
Push the ejection button.
Push laterally (to the left) the
tip-ejector.
Slide and remove the tip-ejector.
To refit
Push the ejection button.
Slide the tip-ejector along the
tip-holder.
Clip and push laterally (to the right) the tip-ejector
on the body of the pipette.
Changing the tip-holder – no tools required
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly and the seals.
Clean, sterilize, or replace the tip-holder.
If necessary, lubricate lightly the piston and its seals
(see below).
Reassemble the pipette (refer to the figure 16).
Tighten the connecting nut (turn counterclockwise).
Refit the tip-ejector (see above).
FIGURE 15
Servicing the piston assembly
You may remove the piston assembly for cleaning purposes only. If the piston assembly is changed, the pipette must be adjusted and calibrated in the Supplier
Service Center. As the models 2µl and 10µl single
channel models contain miniaturized parts, it is best
not to disassemble these pipettes yourself.
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly, O-ring and seal.
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Leave exposed the piston, clean it with isopropanol
or ethanol and lubricate lightly.
For 20µl, 100µl, 200µl Single channel models:
lubricate only the useful part of the piston
(20 ±5 mm length) and the O-ring.
For 1000µl single channel model:
lubricate the piston.
For 5000µl, 10mL single channel model:
disassemble the seals, lubricate their internal part
and lubricate the piston. Do not lubricate the O-ring.
Reassemble the pipette (refer to the figure 16).
Tighten the connecting nut (turn counterclockwise).
Refit the tip-ejector (see above).
Changing the seals
The O-ring and seal are on the piston; if worn or damaged in any way (chemical or mechanical), they must be
replaced. As the models
models
contain miniaturized parts, it is best not to
2µl and 10µl single channel
disassemble these pipettes yourself, please contact
your Supplier Service Center.
The dimensions of the O-ring vary depending on the
model of pipette.
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly, O-ring and seal.
If necessary clean the piston and replace the seal ;
lubricate them lightly. Please place them in the correct order.
Reassemble the pipette (refer to the figure 16).
Tighten the connecting nut (turn counter clockwise).
Refit the tip-ejector (see above).
12B - MAINTENANCE FOR THE MULTICHANNEL MODELS ONLY
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BIOTIX COBRA PIPETTE
FIGURE 16
20µl
100µl
200µl
Color-coded push-button
(volume setting)
Connecting nut
Tip-ejector Tip-holder
1000µl
Piston assembly
5000µl
10mL
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GUIDE
12B - MAINTENANCE FOR THE MULTICHANNEL MODELS ONLY
Routine maintenance will help keep your pipette in good
condition, ensuring a continued high level of performance.
Maintenance is limited to:
• Cleaning or sterilization (see Chapter 13 - Cleaning
and Sterilization)
• Replacing spare parts
• Greasing the piston assembly.
FIGURE 17
Ejector lock
Tip-ejector
19
BIOTIX COBRA PIPETTE
Changing the tip-ejector
To remove the tip-ejector, keep both ejector locks
depressed. Pull the tip-ejector down. To refit the tipejector, gently re-insert the tip-ejector vertically into
the rails of the ejector support. Pull lightly on the tipejector to check the position.
FIGURE 18
Ejector spacer for
10µl models only
13 - CLEANING AND STERILIZATION
LabPRO Pipettes are designed so that the parts normally
in contact with liquid contaminants, can easily be cleaned
and decontaminated. However, because the models 2µl
and 10µl single channel models contain miniaturized
parts, it is best not to disassemble these pipettes
yourself; please contact your Supplier Service Center.
Liquid must never enter the upper part (handle) of anypipette.
Cleaning for the Single Models only
The pipette must be cleaned, as described below, before it is decontaminated. Soap solution is recommended for cleaning these models.
External cleaning
Remove the tip-ejector.
Wipe the tip-ejector with a soft-cloth or lint-free
tissue impregnated with soap solution.
20
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GUIDE
Wipe the entire pipette with a soft-cloth or lint-free
tissue impregnated with soap solution, to remove all
dirty marks. If the pipette is very dirty, a brush with
soft plastic bristles may be used.
Wipe the entire pipette and the tip-ejector with a soft
cloth or lint-free tissue soaked with distilled water.
Refit the tip-ejector and allow the pipette to dry.
Internal cleaning
The following components only can be immersed in a
cleaning solution: connecting nut, tip-ejector, tip-holder,
piston assembly, seal and O-ring.
Disassemble the pipette as described in the chapter
12A - Maintenance.
Set aside the upper part in a clean, dry place.
Clean the individual components of the lower part
of the pipette using an ultrasonic bath (20 minutes
at 50°C) or with a soft-cloth. Note that the piston
assembly and seals must be degreased with isopropanol or ethanol before being immersed in another ultrasonic bath. Small round brushes with soft
plastic bristles may be used to clean the interior of
the tip-holder.
Rinse the individual components with distilled water.
Leave the parts to dry by evaporation or wipe them
with a clean soft-cloth or lint-free tissue.
Reassemble the pipette as described in the chapter
12A - Maintenance.
Cleaning for the Multichannel Models only
The pipette must be cleaned,
as described below, before
it is decontaminated. Soap
solution is recommended for
FIGURE 19
Tip-ejector
cleaning these models.
The following components
only can be immersed in a
cleaning solution: tip-ejector
and ejector spacer.
Remove the tip-ejector
and the ejector spacer.
Ejector spacer
for 10µl models only
21
BIOTIX COBRA PIPETTE
Immerse the tip-ejector, and ejector spacer in the
cleaning solution or wipe them with a soft cloth or
free tissue impregnated with the cleaning solution.
Rinse the components with distilled water.
Wipe the entire pipette with a soft cloth or lint-free
tissue impregnated with the cleaning solution.
Wipe it with distilled water.
Leave the parts to dry by evaporation or wipe them
with a clean soft-cloth or lint-free tissue.
Refit the tip-ejector as described in “Changing the
tip-ejector”.
Chemical Decontamination
You may choose to decontaminate your pipette chemically, in accordance with your own procedures. Whatever
decontaminant you use, check with the supplier of the
decontaminant that it is compatible with stainless steel
and the plastics used in the construction of the pipette:
PBT (Polybutylene Terephtalate), PC (Polycarbonate),
PC/PBT (Polycarbonate/Polybutylene Terephtalate),
PEEK (Polyetheretherkitone), PEI (Polyetherimide), POM
(Polyoxymethylene), PPS (Polyphenylene Sulfide), PVDF
(Polyvinylidene Fluoride), or PP (Polypropylene).
lint-
For the single models:
Upper Part (handle)
Wipe the upper part (handle) of the pipette with a
soft cloth or lint-free tissue impregnated with the
chosen decontaminant.
Wipe the upper part of the pipette with a soft cloth
or lint-free tissue soaked with distilled water or
sterile water.
Lower Part (Volumetric module)
The following components only can be immersed in a
decontaminant solution: connecting nut, tip-ejector,
tip-holder.
Piston assembly and seals must be degreased with
methyl alcohol before being immersed in decontamination solution in separate vessel.
Disassemble the pipette as described in the chapter
12A.
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GUIDE
Immerse tip-ejector, tip-holder and connecting nut
in the cleaning solution.
Degrease the piston assembly, the seals and im-
merse them in another vessel.
Rinse each component with distilled water.
Leave the parts to dry by evaporation (or wipe with
a soft cloth the tip-ejector, the tip-holder and connecting nut).
Lubricate the piston assembly and the seals.
Reassemble the piston assembly, the tip-holder
and the tip-ejector.
For the multichannel models:
The following components only can be immersed in a
decontamination solution: tip-ejector and ejector spacer
(for 10µl models only).
Remove the tip-ejector and the ejector spacer.
Immerse the tip-ejector and ejector spacer in the
decontamination solution or wipe them with a soft
cloth or lint-free tissue impregnated with the decontamination solution.
Rinse the components with distilled water.
Wipe the entire pipette with a soft cloth or lint-free
tissue impregnated with the decontamination solution.
Wipe it with distilled water.
Leave the parts to dry by evaporation or wipe them
with a clean soft-cloth or lint-free tissue.
Refit the tip-ejector as described in “Changing the
tip-ejector”.
Sterilization
The entire autoclavable range of pipettes can be
sterilized by steam autoclaving at 121°C (252°F), 1 atm
for 20 minutes. The single and multichannel pipettes
can be autoclaved without special precautions. Use of a
bag is not recommended in order to improve the drying
of the pipette.
After autoclaving, check the connecting nut is fully
tightened and screw it if it is not the case.
The pipette
needs to dry completely and cool down to room
temperature. (1/2 day if your autoclave has a dry cycle
or otherwise overnight before use). The piston does not
23
BIOTIX COBRA PIPETTE
need to be lubricated after autoclaving, except if grease
was removed during cleaning. Gravimetric checking is
recommended after every 5 autoclave cycles for single
pipettes and after 1 cycle for multichannel pipettes.
14 - SPECIFICATIONS
LabPRO pipettes are high quality. These pipettes are
compatible with universal tips.
Checking and recalibrating your pipette with the tips
that you use may be needed.
Each pipette is inspected and validated by qualified
technicians in accordance with DKSH Quality System.
DKSH declares that its manufactured pipettes comply
with the requirements of the ISO8655 standard, by type
testing.
The adjustment is carried out under strictly defined and
monitored conditions (described in the internal manufacturer procedure wich are based on the ISO8655).
Single models
SPECIFICATIONS - Error limits
Model Volume Volume Systematic error Random error
range (µL) (µL) (µL) (%) (µL) (%)
2µl 0.2 - 2 0.2 ± 0.026 ± 13.2 ≤ 0.013 ≤ 6.6
2 ± 0.033 ± 1.7 ≤ 0.015 ≤ 0.8
10µl 1 - 10 1 ± 0.035 ± 3.5 ≤ 0.013 ≤ 1.3
10 ± 0.110 ± 1.1 ≤ 0.044 ≤ 0.4
20µl 2 - 20 2 ± 0.11 ± 5.5 ≤ 0.033 ≤ 1.7
20 ± 0.20 ± 1.0 ≤ 0.066 ≤ 0.3
100µl 10 - 100 10 ± 0.39 ± 3.9 ≤ 0.11 ≤ 1.1
100 ± 0.80 ± 0.8 ≤ 0.17 ≤ 0.2
200µl 20 - 200 20 ± 0.55 ± 2.8 ≤ 0.22 ≤ 1.1
200 ± 1.60 ± 0.8 ≤ 0.33 ≤ 0.2
1000µl 100 - 1 000 100 ± 3.3 ± 3.3 ≤ 0.7 ≤ 0.7
1000 ± 8.0 ± 0.8 ≤ 1.7 ≤ 0.2
5000µl 500 - 5 000 500 ± 13 ± 2.6 ≤ 3.3 ≤ 0.7
5000 ± 33 ± 0.7 ≤ 8.8 ≤ 0.2
10mL 1000 - 10000 1000 ± 33 ± 3.3 ≤ 6.6 ≤ 0.7
10000 ± 60 ± 0.6 ≤ 17.6 ≤ 0.2
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USER’S
USER’S
GUIDE
GUIDE
The data given in the tables are achieved with Gilson
standard length series pipette tips. If you are using other
10 µL extended length series pipette tips, you will need
to recalibrate your pipette to comply with specifications.
Multichannel models
SPECIFICATIONS - Error limits
Model Volume Volume Systematic error Random error
range (µL) (µL) (µL) (%) (µL) (%)
8X-10µl 0.5-10 0.5 ± 0.09 ± 17.6 ≤ 0.04 ≤ 8.8
12X-10µl 10 ± 0.22 ± 2.2 ≤ 0.11 ≤ 1.1
8X-20µl 2-20 2 ± 0.11 ± 5.5 ≤ 0.09 ≤ 4.4
12X-20µl 20 ± 0.40 ± 2.0 ≤ 0.17 ≤ 0.8
8X-200µl 20-200 20 ± 0.55 ± 2.8 ≤ 0.28 ≤ 1.4
12X-200µl 200 ± 2.20 ± 1.1 ≤ 0.55 ≤ 0.3
8X-300µl 20-300 20 ± 1.10 ± 5.5 ≤ 0.39 ≤ 1.9
12X-300µl 300 ± 3.30 ± 1.1 ≤ 1.10 ≤ 0.4
The data given in the tables conform to the
ISO8655-2 Standard. With a precise pipetting
technique (see Chapter 7 - General guidelines
for good pipetting) the 2µl single channel model
may be used to aspirate volumes as low as
0.1 µL and the 10µl single channel model as low
as 0.5 µL.
15A- SPARE PARTS FOR SINGLE
MODELS
Description Quantity 2µl
A
B
C
D
E
F1 F2
F2
Push button 5 FE07004
Connecting nut 5 FE07001
Piston assembly 1 FE07040
Seal + O-ring 5 FE07050
Tip holder 5 FE07013
Tip-ejector assembly 5 FE07059
Ejector adapter 1 FE07034
25
BIOTIX COBRA PIPETTE
FIGURE 20
F1
A
B
C
D
E
26
F2
10µl
Description Quantity 10µl
A
B
C
D
E
F1 F2
F2
Push button 5 FE07005
Connecting nut 5 FE07001
Piston assembly 1 FE07041
Seal Guide + O-ring 5 FE07051
Tip holder 5 FE07015
Tip-ejector assembly 5 FE07059
Ejector adapter 1 FE07034
20µl and 100µl
Description Quantity 20µl 100µl
A
B
C
D
E
F1
Push button 5 FE07006 FE07007
Connecting nut 5 FE07001 FE07001
Piston assembly 1 FE07042 FE07043
Seal Guide + O-ring 5 FE07052 FE07053
Tip holder 5 FE07017 FE07019
Tip-ejector assembly 5 FE07030 FE07031
200µl and 1000µl
Description Quantity 200µl 1000µl
A
B
C
D
E
F1
Push button 5 FE07008 FE07009
Connecting nut 5 FE07001 FE07001
Piston assembly 1 FE07044 FE07045
Seal Guide + O-ring 5 FE07054 FE07055
Tip holder 5 FE07021 FE07023
Tip-ejector assembly 5 FE07032 FE07033
5000µl and 10ml
Description Quantity 5000µl 10ml
A
C
D
E
* Not shown.
Push button 5 FE07010 FE07011
Piston assembly 1 FE07047 FE07049
O-ring 5 FE07056 FE07057
Tip holder 5 FE07025 FE07027
Filter for 5000µl/
10ml*
USER’S
USER’S
GUIDE
GUIDE
10 FE07058 FE07058
27
BIOTIX COBRA PIPETTE
15B - SPARE PARTS FOR
MULTICHANNEL MODELS
FIGURE 21
F3
F4
Tip-ejector (12 channels)
Tip-ejector (8 channels)
G2
Ejector spacer (12 channels)
for short collar tips
8x10µl and 12x10µl
Description Quantity 8x10µl 12x10µl
A
F3 F4
G1 G2
Push button 5 FE07005 FE07005
Tip-ejector 1 FE07038 FE07039
Ejector spacer for short
collar tips
8x20µl and 12x20µl
Description Quantity 8x20µl 12x20µl
A
F3 F4
Push button 5 FE07006 FE07006
Tip-ejector 1 FE07038 FE07039
8x200µl and 12x200µl
Description Quantity 8x200µl 12x200µl
A
F3 F4
Push button 5 FE07008 FE07008
Tip-ejector 1 FE07038 FE07039
G1
Ejector spacer (8 channels)
for short collar tips
1 FE07036 FE07037
28
8x300µl and 12x300µl
Description Quantity 8x300µl 12x300µl
A
F3 F4
Push button 5 FE07012 FE07012
Tip-ejector 1 FE07038 FE07039
USER’S
USER’S
GUIDE
GUIDE
29
BIOTIX COBRA PIPETTE
MAINTENANCE & CALIBRATION
DKSH recommends pipette calibration and maintenance at least once
annually by an authorized service provider. Please contact DKSH directly.
Please refer to the last page detailling local DKSH offices list.
30
USER’S
USER’S
GUIDE
GUIDE
WARRANTY
DKSH warrants this pipette against defects in material under normal use
and service for a period of 36 months from the date of purchase. An extended warranty is possible on request. It extends the standard warranty
coverage for a maximum of 2 years on selected components.
This warranty shall not apply to pipettes which are subject to abnormal
use, and/or improper or inadequate maintenance (contrary to the
recommendations given in the User’s guide), including, but not limited to
pipettes which have been subjected to physical damage, improper handling,
spillage or exposure to any corrosive environment. This warranty shall also
be void in the event pipettes are altered or modified by any party other than
DKSH.The company’s sole liability under this warranty shall be limited to
repair or replace any defective components of pipettes or refund of the
purchase price paid for such pipettes.
THE FOREGOING WARRANTY IS EXCLUSIVE AND DKSH HEREBY
DISCLAIMS ALL OTHER WARRANTIES, WHETHER EXPRESS OR
IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY
AND ANY WARRANTIES OF FITNESS FOR A PARTICULAR
PURPOSE, UNDER NO CIRCUMSTANCES SHALL DKSH BE LIABLE
FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT OR INCIDENTAL
DAMAGES ARISING OUT OF ANY BREACH OF ANY EXPRESS OR
IMPLIED WARRANTY.
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BIOTIX COBRA PIPETTE
Australia
DKSH Australia Pty Ltd
+61 3 9554 6666
Cambodia
DKSH (Cambodia) Ltd
+855 23 212 838
China
DKSH (China) Co., Ltd.
+86 21 5830 0518
Indonesia
PT DKSH Indonesia
+62 21 2988 8557
Japan
DKSH Japan K.K
+81 3 5730 7340
Korea
DKSH Korea Ltd.
+82 2 2192 9500
Laos
DKSH Laos Company Ltd.
+856 2145 3100
Myanmar
DKSH (Myanmar) Ltd.
+95 1 565 270
New Zealand
DKSH New Zealand Ltd
+64 6 356 5323
Philippines
DKSH Philippines Inc
+63 2 864 1677
Singapore
DKSH Singapore Pte Ltd
+65 6471 1466
Taiwan
DKSH Taiwan Ltd.
+886 2 8752 6666
Thailand
DKSH (Thailand) Limited
+66 269 54112
Vietnam
DKSH Technology Co.,Ltd (Vietnam)
+84 8 3812 5848
Malaysia
DKSH Malaysia Sdn Bhd
+60 3 7882 8888
LT801578/A -©2017 DKSH, All rights reserved April 2017
English Printed in France
Specifications subject to change without notifications - errors omitted.
www.dksh.com
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