Dionex UltiMate 3000 Series Operating Instructions Manual
UltiMate 3000 Series
SD, RS, and BM Pumps
Operating Instructions
(Original Operating Instructions)
Revision: 1.4
Date: September 2010
© 2010 Dionex
Doc. No. 4820.4001
UltiMate 3000 Series:
SD, RS, and BM Pumps
Operating Instructions
Declaration of Conformity
Product: Pump
Types: ISO-3100SD, ISO-3100BM
HPG-3200SD, HPG-3200RS, HPG-3400SD, HPG-3400RS
LPG-3400SD, LPG-3400RS, LPG-3400BM
DGP-3600SD, DGP-3600RS, DGP-3600BM
Dionex GmbH herewith declares conformity of the above products with the
respective requirements of the following regulations:
Machinery Directive 2006/42/EC
Low-Voltage Directive 2006/95/EC
EMC Directive 2004/108/EC
The safety of the machinery was evaluated based on the following standard:
EN-ISO-14121-1
Safety of machinery - risk assessment, part 1
The electrical safety of the products was evaluated based on the following
standard:
EN 61010-1: 2002
Safety requirements for electrical equipment for measurement, control and
laboratory use, Part 1: General Requirements
The Electromagnetic Compatibility (EMC) of the products was evaluated
based on the following standard:
DIN EN 61326: 2006
Electrical equipment for measurement, control and laboratory use
EMC Requirements
Person responsible for the technical CE documentation: Burkhard Seyferth
at Dionex Softron GmbH, Dornierstrasse 4, D-82110 Germering
This declaration is issued for the manufacturer
Dionex Softron GmbH
Dornierstraße 4
D-82110 Germering
by the President, Dr. Peter Jochum.
August 10, 2010
Operating Instructions Page I
UltiMate 3000 Series:
SD, RS, and BM Pumps
Page II Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
Operating Instructions Page III
UltiMate 3000 Series:
SD, RS, and BM Pumps
Page IV Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
Operating Instructions Page V
UltiMate 3000 Series:
SD, RS, and BM Pumps
Page VI Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
Table of Content
1 Introduction................................................................................................................... 1
1.1 How to Use this Manual ........................................................................................1
1.2 Safety Information .................................................................................................2
1.2.1 Symbols on the Pump and in the Manual......................................................... 2
1.2.2 General Safety Precautions............................................................................... 3
1.2.3 Consignes Générales de Sécurité...................................................................... 6
1.3 Intended Use .......................................................................................................... 9
1.4 Federal Communications Commission (FCC) Note.............................................. 9
2 Overview...................................................................................................................... 11
2.1 Unit Description................................................................................................... 11
2.2 Pump Configurations ...........................................................................................12
2.2.1 Overview ........................................................................................................ 12
2.2.2 Combinations of UltiMate 3000 Pumps and Solvent Racks .......................... 13
2.2.3 Special Information for Biocompatible Pumps ..............................................14
2.3 Operating Principle.............................................................................................. 15
2.4 Front Panel Elements........................................................................................... 16
2.5 Rear Panel............................................................................................................17
2.5.1 Power Switch.................................................................................................. 18
2.5.2 Fuse Cartridge ................................................................................................ 18
2.5.3 USB Connector............................................................................................... 18
2.5.4 Digital I/O....................................................................................................... 19
2.5.5 Solvent Rack................................................................................................... 19
2.6 Fluid Connections................................................................................................ 20
2.7 Rear Seal Wash System .......................................................................................21
2.8 Mixing System and Inline-Filter.......................................................................... 22
2.9 Purge Unit............................................................................................................23
2.10 Leak Sensor.......................................................................................................... 23
2.11 Vacuum Degasser ................................................................................................ 24
2.12 Pulse Damper....................................................................................................... 24
2.13 Chromeleon Software .......................................................................................... 25
2.14 Wellness, Predictive Performance and Diagnostics ............................................26
3 Installation................................................................................................................... 27
3.1 Facility Requirements.......................................................................................... 27
3.2 Unpacking............................................................................................................ 27
3.3 Positioning the Pump in the UltiMate 3000 System............................................ 28
3.4 Connecting the Pump...........................................................................................30
3.4.1 General Information .......................................................................................30
3.4.2 Connecting the USB Cable............................................................................. 30
Operating Instructions Page i
UltiMate 3000 Series:
SD, RS, and BM Pumps
3.4.3 Connecting the Power Cord............................................................................31
3.4.4 Connecting the Solvent Rack and Digital I/O ................................................31
3.5 Setting Up the Pump in Chromeleon ...................................................................32
3.5.1 Loading the USB Driver for the Pump...........................................................32
3.5.2 Installing the Pump.........................................................................................34
3.5.3 Configuring the Pump.....................................................................................35
3.5.3.1 Initial Installation.....................................................................................35
3.5.3.2 Changing the Configuration Properties ...................................................42
3.6 Connecting a Dionex ESA Detector to the Pump................................................43
4 Preparation for Operation (Startup) ........................................................................45
4.1 Overview of Actions............................................................................................45
4.2 General Precautions for Connecting Capillaries.................................................. 47
4.3 Solvent Reservoirs ...............................................................................................48
4.3.1 General Notes ................................................................................................. 48
4.3.2 Connecting the Solvent Reservoirs.................................................................49
4.3.2.1 All pumps except ISO-3100BM..............................................................49
4.3.2.2 ISO-3100BM ...........................................................................................50
4.4 Setting Up the Rear Seal Wash System ...............................................................51
4.5 Connecting Drain Tubing ....................................................................................53
4.6 Purging the Pump.................................................................................................54
4.6.1 Purging the Pump Manually ...........................................................................55
4.6.2 Purging the Pump By Using the Autosampler................................................57
4.7 Equilibrating the System......................................................................................58
5 Operation and Maintenance ...................................................................................... 61
5.1 Power-Up .............................................................................................................61
5.2 Status Screens ......................................................................................................62
5.3 Operation with Chromeleon................................................................................. 64
5.3.1 Connecting to Chromeleon.............................................................................64
5.3.2 Direct Control .................................................................................................65
5.3.3 Automated Control ......................................................................................... 68
5.4 Function Keys and Menus on the Pump Display.................................................70
5.4.1 Showing the Function Keys............................................................................70
5.4.2 Pump Menus ...................................................................................................72
5.4.2.1 General Menu Layout and Structure .......................................................73
5.4.2.2 Main Menu ..............................................................................................74
5.4.2.3 Control Menu...........................................................................................75
5.4.2.4 Preferences Menu ....................................................................................75
5.4.2.5 Diagnostics Menu....................................................................................76
5.4.2.6 Configuration Menu ................................................................................76
5.5 Information for Operating the Pump....................................................................77
5.5.1 Choosing the Solvents ....................................................................................77
5.5.2 Linking the Pump to the Autosampler............................................................80
Page ii Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
5.5.3 Setting the Flow Rate, Flow Acceleration, and Flow Deceleration ............... 81
5.5.4 Setting the Pressure Limits............................................................................. 82
5.5.5 Recording the Pump Pressure......................................................................... 83
5.5.6 Rear Seal Wash System.................................................................................. 83
5.5.6.1 Working with Rear Seal Washing...........................................................83
5.5.6.2 Choosing the Seal-Washing Liquid......................................................... 84
5.5.6.3 What happens …. .................................................................................... 84
5.5.7 Purging the Pump ........................................................................................... 85
5.5.8 Detecting Liquid Leaks in the Pump..............................................................86
5.5.9 Adjusting the Screen Brightness or Contrast.................................................. 87
5.5.10 SmartStartup and SmartShutdown..................................................................87
5.5.11 Vacuum Degasser (LPG-3400 and SRD-3x00)..............................................88
5.5.11.1 General Notes for Degasser Operation.................................................... 88
5.5.11.2 Turning the Degasser on and off ............................................................. 89
5.5.12 General Precautions for Operating an ISO-3100BM .....................................90
5.6 Special Chromeleon Functions............................................................................91
5.6.1 Predictive Performance .................................................................................. 91
5.6.2 Pump Diagnostics........................................................................................... 93
5.6.3 Setting a Gradient Curve ................................................................................ 94
5.6.4 Liquid Level Monitoring for Solvent Reservoirs and Waste Container.........94
5.6.5 Using the Digital Inputs and Outputs (Digital I/O)........................................ 96
5.6.6 Operational Qualification and Performance Qualification............................. 96
5.7 Shutting Down the Pump..................................................................................... 97
5.8 Routine and Preventive Maintenance ..................................................................99
6 Troubleshooting ........................................................................................................ 103
6.1 Overview............................................................................................................ 103
6.2 Pump Block Status Indicator ............................................................................. 104
6.3 Messages on the Pump Display ......................................................................... 105
6.4 Chromeleon Diagnostics Messages ................................................................... 112
6.5 Operating Problems ........................................................................................... 114
6.6 Checking the Compression Values.................................................................... 121
7 Service
........................................................................................................................ 123
7.1 General Notes .................................................................................................... 123
7.2 Eliminating Leakage.......................................................................................... 124
7.3 Rear Seal Wash System .....................................................................................125
7.3.1 Inspecting the Rear Seal Wash System for Leakage....................................125
7.3.2 Replacing the Peristaltic Tubing ..................................................................126
7.3.3 Replacing the Detector ................................................................................. 127
7.3.4 Cleaning the Detector...................................................................................127
7.4 Replacing the Check Valve Cartridges.............................................................. 128
7.5 Pistons and Piston Seals..................................................................................... 130
7.5.1 Visually Inspecting the Pump for Piston Seal Leakage................................134
7.5.2 Pump Head and Pistons................................................................................135
Operating Instructions Page iii
UltiMate 3000 Series:
SD, RS, and BM Pumps
7.5.2.1 Removing the Pump Head and Pistons..................................................135
7.5.2.2 Installing the Pistons and Pump Head ...................................................137
7.5.3 Replacing the Piston Seals............................................................................139
7.5.3.1 Disassembling the Pump Head and Removing the Piston Seals ...........140
7.5.3.2 Cleaning the Pistons ..............................................................................141
7.5.3.3 Installing the Piston Seals and Reassembling the Pump Head..............142
7.5.3.4 Recommended Actions after Piston Seal Replacement.........................144
7.5.3.5 Replacing the Piston Seals in the Plate of the Seal Wash System.........145
7.6 Mixing System...................................................................................................146
7.6.1 Capillary Mixer.............................................................................................146
7.6.2 Static Mixer ..................................................................................................147
7.6.2.1 Replacing the Static Mixer ....................................................................147
7.6.2.2 Checking the Static Mixer for Permeability..........................................148
7.7 Inline Filter.........................................................................................................149
7.7.1 Replacing the Inline Filter (ISO-3100)......................................................... 149
7.7.2 Inline Filter (LPG-3400BM, DGP-3600BM)...............................................150
7.7.2.1 Replacing the Inline Filter.....................................................................150
7.7.2.2 Replacing the Filter Frit in the Inline Filter...........................................151
7.7.3 Checking the Inline Filter for Permeability..................................................152
7.8 Replacing the Purge Valve Knob....................................................................... 153
7.9 Testing the Pump for Leakage ...........................................................................154
7.10 Vacuum Degasser (Rinsing the Degassing Channels).......................................157
7.11 Replacing the Main Power Fuses.......................................................................158
7.12 Updating the Pump Firmware............................................................................ 159
8 Pump-Specific Information...................................................................................... 161
8.1 ISO-3100............................................................................................................162
8.1.1 ISO-3100SD .................................................................................................162
8.1.1.1 Interior Components..............................................................................162
8.1.1.2 Flow Schematic ..................................................................................... 163
8.1.1.3 Operating Principle (Schematics)..........................................................164
8.1.2 ISO-3100BM ................................................................................................165
8.1.2.1 Interior Components..............................................................................165
8.1.2.2 Flow Path............................................................................................... 166
8.1.3 Operating Principle (Schematics)................................................................. 167
8.2 LPG-3400........................................................................................................... 168
8.2.1 Interior Components .....................................................................................168
8.2.2 Flow Path......................................................................................................169
8.2.3 Operating Principle (Schematics)................................................................. 171
8.3 DGP-3600 ..........................................................................................................172
8.3.1 Interior Components .....................................................................................172
8.3.2 Flow Path......................................................................................................173
8.3.3 Operating Principle (Schematics)................................................................. 175
Page iv Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
8.4 HPG-3200 ..........................................................................................................176
8.4.1 Interior Components..................................................................................... 176
8.4.2 Flow Path...................................................................................................... 177
8.4.3 Operating Principle (Schematics)................................................................. 178
8.5 HPG-3400 ..........................................................................................................179
8.5.1 Interior Components..................................................................................... 179
8.5.2 Flow Path...................................................................................................... 180
8.5.3 Possible Gradient Combinations and Operating Principle (Schematics) .....181
9 Optimizing the Pump for Specific Applications ....................................................183
10 Technical Information.............................................................................................. 187
11 Accessories, Consumables, and Spare Parts ..........................................................191
11.1 Standard Accessories.........................................................................................191
11.2 Optional Accessories ......................................................................................... 197
11.3 Consumables and Spare Parts............................................................................ 199
12 Reference Information .............................................................................................207
12.1 Chemical Resistance of PEEK........................................................................... 207
12.2 Solvent Miscibility............................................................................................. 210
12.3 Properties of Common Solvents ........................................................................ 211
12.4 Safety Information about Flammable Solvents .................................................212
13 Appendix.................................................................................................................... 215
13.1 Digital I/O (Pin Assignment)............................................................................. 215
13.2 Solvent Rack (Pin Assignment)......................................................................... 216
14 Index........................................................................................................................... 217
Operating Instructions Page v
UltiMate 3000 Series:
SD, RS, and BM Pumps
Page vi Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
1 Introduction
1.1 How to Use this Manual
The layout of this manual is designed to provide quick reference to the sections of interest
to the reader. However, in order to obtain a full understanding of the pump, Dionex
recommends that you review the manual thoroughly before beginning operation.
Almost all descriptions in the manual apply to all SD, RS, and BM pumps in the UltiMate
3000 pump series. Therefore, the term "the pump" is used throughout the manual. If some
detail applies to only one pump model or version, the model (version) is identified by name. If
only the pump name is indicated, for example, HPG-3200, the related information applies to
all pump versions (that is, for the example, to the HPG-3200SD and HPG-3200RS).
The same applies to the descriptions of the Viper™ capillary connections throughout this
manual. They apply also to the nanoViper™ capillary connections if not otherwise stated.
Notes: The device configuration may vary; therefore, not all descriptions necessarily
apply to your particular pump.
The optical design of some components may differ from the representation in the
pictures in this manual, but this does not affect the descriptions.
The descriptions in this manual refer to firmware version 3.00 and Chromeleon
6.80 Service Release 10. If you want to operate the pump with Chromeleon 7,
note the information on page 25.
The information contained in this manual should not be construed as a commitment by
Dionex. Dionex assumes no responsibility for any errors that may appear in this document.
This document is believed to be complete and accurate at the time of publication. In no
event shall Dionex be liable for incidental or consequential damages in connection with or
arising from the use of this document.
The information contained in this document is subject to change without notice.
®
CHROMELEON
Dionex Corporation in the United States of America and/or other countries. Viper™ and
nanoViper™ are trademarks of Dionex Corporation in the United States of America and/or
other countries. All other trade or company names mentioned are subject to the copyright and
the property and trademark rights of the respective companies.
, UltiMate®, Corona®, and Coulochem® are registered trademarks of
Operating Instructions Page 1
UltiMate 3000 Series:
SD, RS, and BM Pumps
All rights reserved, including those for photomechanical reproduction and storage on
electronic media. No part of this publication may be copied or distributed, transmitted,
transcribed, stored in a retrieval system, or transmitted into any human or computer language,
in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or
disclosed to third parties without the express written permission of Dionex.
1.2 Safety Information
The CE Mark label and cTUVus Mark safety label on the pump indicate that the pump is
compliant with the related standards ( pages I and II).
1.2.1 Symbols on the Pump and in the Manual
The table shows the symbols used on the pump:
Symbol Description
Alternating current—Courant alternatif
˜
Power supply is on (-)—L'instrument est mis sous tension (-) and
Power supply is off (O)—L'instrument est mis hors tension (O)
Refer to the Operating Instructions to prevent risk of harm to the operator and to
protect the instrument against damage.
Référez-vous à ce manuel pour éviter tout risque de blessure à l'opérateur et/ou
protéger l'instrument contre tout dommage.
Label according to the "Measures for Administration of the Pollution Control of
Electronic Information Products" (China RoHS) guideline
Étiquette "Measures for Administration of the Pollution Control of Electronic
Information Products" (China RoHS)
WEEE (Waste Electrical and Electronic Equipment) label—For more
information, see the WEEE Information section in the "Installation and
Qualification Documents for HPLC Systems" binder. Étiquette WEEE (Waste
Electrical and Electronic Equipment) —Pour plus d'informations, référez-vous au
chapitre WEEE Information dans le classeur "Installation and Qualification
Documents for HPLC Systems".
Page 2 Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
At various points throughout the manual, messages of particular importance are indicated
by certain symbols:
Tip: Indicates general information, as well as information intended to
optimize the performance of the instrument.
Important: Indicates that failure to take note of the accompanying information
could cause wrong results or may result in damage to the module.
Important: Indique que ne pas tenir compte de l'information jointe peut conduire
à de faux résultat ou endommager l'instrument.
Warning: Indicates that failure to take note of the accompanying information
may result in personal injury.
Avertissement: Indique que ne pas tenir compte de l'information jointe peut
entraîner des blessures corporelles.
1.2.2 General Safety Precautions
When working with analytical instrumentation, you should know the potential hazards of
using chemical solvents. Wear appropriate protective clothing.
Tips: Before initial operation of the pump, make sure that you are familiar with the
contents of this manual.
Observe any warning labels on the pump and see the related sections in these
Operating Instructions.
To avoid the possibility of personal injury and damage to the instrument, observe the
following general safety precautions when operating the pump or carrying out any
maintenance work:
Install the HPLC system in a well-ventilated laboratory. If the mobile phase includes
volatile or flammable solvents, do not allow them to enter the workspace.
For minimum interference effects, all components of the analytical system should be
connected to the same mains output (same phase).
The pump is primed with 2-propanol. During initial operation of the pump, make sure that
the solvents used are miscible with 2-propanol. Otherwise, follow the appropriate
intermediate steps.
When lifting or moving the pump, always lift by the sides of the instrument. Lifting the
pump by the front panel may damage the front panel door.
Operating Instructions Page 3
UltiMate 3000 Series:
SD, RS, and BM Pumps
Do not place any heavy objects on the open front panel door. This may damage the door.
Always set a lower pressure limit for the HPLC pump. This prevents damage resulting
from leakage or from running the pump dry over a longer period. Activate liquid level
monitoring for the solvent reservoirs ( page 94).
To avoid that the pressure calibration of the pump is impaired, turn on the pump only
when the pump pressure is down. To ensure that the pressure is down, open the purge
valve before turning on the pump. If you are operating an ISO-3100BM, observe the
precautions on page 90.
Never run the pump dry. Damage to the pistons or the piston seals could result.
Before you start operating the pump, check the liquid level in the wash liquid reservoir
and refill the reservoir as needed. After turning on the pump, wait until the wash liquid
has passed all pump heads.
Always use fresh rear seal wash liquid.
Dionex advises against recycling the solvents. This may impair the performance of the
seals.
When connecting the capillaries, make sure that the connectors are free from
contaminants. Even minute particles may cause damage to the system, for example, to the
column.
After operation, rinse out buffers and solutions that form peroxides.
Before switching from buffer to organic solution, rinse the pump thoroughly with
deionized water.
When switching to another solvent, ensure that the new solvent is miscible with the one
contained in the pump. Otherwise, the pump can be damaged; for example, by
flocculation.
If the pump flow is interrupted for longer periods (> 1 hour), turn off the lamps in any UV
or RF detector connected to the pump. This will prevent evaporation in the flow cell.
If you want to connect an ESA Corona or Coulochem III detector to the pump, refer to
page 43 for details.
Always use the frits recommended by Dionex to prevent particulate matters from entering
the HPLC system. Using other frits may considerably affect the system performance.
Do not use stainless steel frits with the BM pumps; this renders the biocompatibility void.
Frits are used on the drawing side (in the solvent reservoirs) and on the high-pressure side
(in the inline filter).
If the mobile phase includes volatile or flammable solvents, avoid open flames and
sparks.
Page 4 Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
If a leak occurs, turn off the pump and remedy the situation immediately.
Disconnect the pump from all power sources before removing the panels. When the
panels are removed, dangerous electrical connections will be exposed. The enclosure
should be opened only by authorized service personnel.
Always replace blown fuses with original spare part fuses from Dionex ( page 191).
Replace faulty power cords and communication cables.
Many organic solvents and buffers are toxic. Know the toxicological properties of all
mobile phases that you are using.
The toxicological properties of many samples may not be well known. If you have any
doubt about a sample, treat it as if it contains a potentially harmful substance.
Wear goggles when handling mobile phases or operating the pump. An eyewash facility
and a sink should be close to the unit. If any mobile phase splashes on the eyes or skin,
wash the affected area and seek medical attention.
Dispose of waste mobile phase in an environmentally safe manner that is consistent with
all local regulations. Do not allow flammable or toxic solvents to accumulate. Follow a
regulated, approved waste disposal program. Never dispose of flammable or toxic
solvents through the municipal sewage system.
Use only standard solvents (HPLC grade) and buffers that are compatible with all parts
that may be exposed to solvents.
In an UltiMate 3000 system, some components are made of PEEK™. While this polymer
has superb chemical resistance to most organic solvents, it tends to swell when in contact
with trichlormethane (CHCl3), dimethyl sulfoxide (DMSO), or tetrahydrofuran (THF). In
addition, it is attacked by concentrated acids, such as, sulfuric acid and nitric acid or a
mixture of hexane, ethyl acetate, and methanol. (Swelling or attack by concentrated acids
is not a problem with brief flushing procedures.)
Do not use PEEK tubing that is stressed, bent, or kinked.
Avoid looking directly into the pump light LED and do not use light focusing instruments
for viewing the light beam. The high luminosity of the lamp can be harmful to the eyes.
Before interrupting operation for several days or more or when preparing the pump for
transport, observe the precautions for shutting down the pump ( page 97).
Use original Dionex spare parts only. Substituting non-Dionex parts or using non-Dionex
accessories m
ay impair the performance of the instrument.
Do not use the pump in ways other than those described in these operating instructions.
Operating Instructions Page 5
UltiMate 3000 Series:
SD, RS, and BM Pumps
1.2.3 Consignes Générales de Sécurité
Veuillez noter: Avant de commencer à utiliser la pompe, assurez-vous que vous
vous êtes familiarisés avec le contenu de ce manuel.
Observez des étiquettes d'avertissement sur l'appareil et référez-vous
aux sections correspondantes dans ce mode d'emploi.
Veuillez observer les consignes générales de sécurité suivantes lorsque vous utilisez
l'instrument ou que vous procédez à des opérations de maintenance.
Installez le système HPLC dans un laboratoire bien ventilé. Si la phase mobile contient
des solvants volatils ou inflammables, empêchez qu'ils ne pénètrent dans l'espace de
travail.
Afin d'éviter au maximum les interférences, tous les éléments du système analytique
doivent être raccordés à la même ligne secteur (même phase).
La pompe est stockée sous 2-propanol. Au cours démarrage de la pompe, assurez-vous
que les solvants utilisés soient miscibles avec le 2-propanol. Sinon, suivez les étapes
intermédiaires appropriées.
Le panneau avant bascule vers le haut. Afin d'éviter d'endommager la pompe lorsque que
vous la soulevez ou la déplacez, saisissez-la toujours par les côtés de l'unité.
Ne placez aucun objet lourd sur la porte ouverte du panneau avant. Ceci pourrait
endommager la porte.
Réglez toujours une limite de pression minimum pour la pompe HPLC. Ceci prévient les
dommages résultant de fuites ou de long-terme fonctionnement à sec de la pompe.
Activez la surveillance de niveau liquide pour des réservoirs ( page 94).
Afin d'éviter que le calibrage de pression de la pompe ne soit pas entravé, mettez en
marche la pompe seulement quand la pompe est sans pression. Toujours ouvrez la vis de
purge avant mettre la pompe en marche. Si la pompe est une ISO-3100BM, observez les
précautions figurant en page 90.
Ne faites jamais fonctionner la pompe à sec. Il peut en résulter des dommages aux pistons
ou aux joints de piston.
Avant de mettre en marche la pompe, assurez-vous que le réservoir de rinçage du joint
arrière est rempli. Attendez jusqu'à ce que le rinçage du joint arrière ait été pompé par
toutes les têtes de pompe.
Utilisez toujours le liquide frais pour le rinçage du joint arrière.
Dionex déconseille de recycler les solvants. Ceci peut nuire aux performances des joints.
Page 6 Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
Lorsque vous connectez les capillaires, assurez-vous que les raccords sont exempts de
tout contaminant. Même d'infimes particules peuvent causer des dommages au système
(ex. diviseur de débit, vanne de régulation de débit et colonne).
Après utilisation, purgez le système des tampons et des susceptibles de former des
peroxydes.
Lorsque vous passez d’une solution saline à un solvant organique, effectuez un rinçage
intermédiaire de la pompe à l'eau dé-ionisée.
Lorsque vous passez à un autre solvant, assurez-vous que le nouveau solvant soit miscible
avec celui qui se trouve dans la pompe. Dans le cas contraire, la pompe peut être
endommagée; par exemple, par des floculations!
Si le débit de la pompe est interrompu pour des périodes prolongées (> 1 heure), éteignez
les lampes de tout détecteur UV ou RF raccordé à la pompe. Ceci empêchera
l'évaporation dans la cellule.
Si vous utilisez un détecteur ESA Corona ou Coulochem III avec la pompe, observez
les remarques figurant en page 43.
Si vous utilisez des phases mobiles avec une forte teneur en sel, ne faites pas fonctionner
la pompe sans rinçage du joint arrière pendant un temps prolongé (> 5 minutes). Ceci peut
endommager les joints de piston et le piston. Remplacer régulièrement le liquide dans le
réservoir du système de rinçage du joint arrière (au moins une fois par semaine).
Utilisez toujours les frittés recommandés par Dionex afin d'empêcher les particules
étrangères d'entrer dans le système HPLC. Utiliser d'autres frittés peut affecter
considérablement les performances du système.
N'employez pas des frittes d'acier inoxydable avec des versions biocompatible de la
pompe. Ceci rend vide de compatibilité biologique de la pompe.
Si la phase mobile contient des solvants volatils ou inflammables, évitez les flammes nues
et les sources d’étincelles à proximité.
Si une fuite survient, arrêtez l'instrument et résolvez le problème immédiatement.
Quand les capots de protection de l’appareil sont démontés, vous êtes exposés à des
connexions électriques sous haute tension deviennent accessibles. Débranchez la pompe
de toute source d'alimentation électrique avant de retirer les capots. Ne démontez les
capots de protection que si cela est explicitement demandé au cours de ces instructions.
Remplacez toujours les fusibles grillés par des fusibles de rechange d'origine Dionex
( page 191).
Remplacez les cordons d'alimentation électrique et les câbles de communication
défectueux.
Operating Instructions Page 7
UltiMate 3000 Series:
SD, RS, and BM Pumps
De nombreux solvants organiques et solutions salines sont toxiques. Informez-vous des
propriétés toxicologiques de toutes les phases mobiles que vous utilisez. Portez le
vêtement de protection approprié.
Les propriétés toxicologiques de nombreux échantillons peuvent être mal connues. Au
moindre doute concernant un échantillon, traitez-le comme s'il contenait une substance
potentiellement dangereuse.
Portez des lunettes de protection lorsque vous manipulez des phases mobiles ou que vous
utilisez l'instrument. Une installation permettant de se laver les yeux ainsi qu'un lavabo
doivent se trouver à proximité du système. Si une phase mobile, quelle qu'elle soit, entre
en contact avec vos yeux ou votre peau, rincez abondamment la zone affectée à l’eau,
puis.
Débarrassez-vous de tous les déchets de phase mobile de manière écologique,
conformément à la règlementation en vigueur au niveau local. Empêchez impérativement
l'accumulation de solvants inflammables et/ou toxiques. Suivez un programme
d'élimination des déchets règlementé et approuvé. Ne jetez jamais de solvants
inflammables et/ou toxiques dans le système municipal d'évacuation des eaux usées.
Utilisez uniquement des solvants (qualité HPLC) et des solutions salines compatibles
avec les matériaux exposés phase mobiles.
Dans un système UltiMate 3000, certaines composantes sont en PEEK. Bien que ce
polymère présente une excellente résistance chimique à la plupart des solvants
organiques, il a tendance à gonfler lorsqu'il est en contact prolongé avec du chloroforme
(CHCl3), du diméthyle sulfoxyde (DMSO) ou du tétrahydrofurane (THF). De plus, il est
attaqué par des acides concentrés tels que l'acide sulfurique et l'acide nitrique ou d'un
composé du hexane, éthyle acétate et méthanol. (Ces acides peuvent cependant être
utilisés dans le cadre de procédures de nettoyage, à condition que l’exposition soit brève.)
N'utilisez pas de tubes PEEK écrasés, pliés ou abimés.
Ne regardez jamais directement la DEL pour l'éclairage intérieur dans la pompe et ne
regardez pas du faisceau lumineux par des instruments qui focalisent le rayon lumineux.
L'intensité lumineuse de la lampe peut être nocive pour les yeux.
Avant d'interrompre le fonctionnement pendant plusieurs jours ou plus, observez les
précautions figurant en page 97.
Utilisez des pièces de rechange d'origine Dionex. Effectuer des remplacements par des
pièces ne provenant pas de Dionex ou utiliser des accessoires ne provenant pas de Dionex
peut affecter les performances de l'instrument.
N'utilisez pas la pompe de manière autre que celles décrites dans ce manuel.
Page 8 Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
1.3 Intended Use
The pump has been designed to perform equally well as a dependable system for routine
analyses and as a sophisticated research instrument for use in high-performance liquid
chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC)
applications, especially as part of the UltiMate 3000 system. However, the pump can also
be used with other HPLC systems if adequate control inputs and outputs are available. A
PC with USB port is required.
The pump is controlled by the Chromeleon Chromatography Management System. If you
want to operate the pump as part of the UltiMate 3000 system with other data systems, such as
Analyst® (AB Sciex), Compass™/ HyStar™ (Bruker Daltonics), or Xcalibur™ (Thermo
Scientific), contact the Dionex sales organization.
Please note that the pump may be operated only with Dionex accessories and spare parts
( page 191) and within its technical specifications ( page 187).
If there is any question regarding appropriate usage, contact Dionex before proceeding.
Dionex cannot be held liable for any damage, material or otherwise, resulting from
inappropriate or improper use of the pump.
1.4 Federal Communications Commission (FCC) Note
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the U.S. FCC Rules. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this equipment in a residential
area is likely to cause harmful interference, in which case the user will be required to
correct the interference at his expense.
Operating Instructions Page 9
UltiMate 3000 Series:
SD, RS, and BM Pumps
Page 10 Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
2 Overview
2.1 Unit Description
The pump is the heart of the Dionex UltiMate 3000 system. The pump performs equally
well as a flexible and reliable module for routine analysis and sophisticated research tasks
in HPLC and UHPLC and can be used in numerous laboratory environments:
The patented isokinetic pre-compression allows a precise and almost pulse-free flow.
The technical specification meets the highest requirements for flow rate reproducibility,
zero pulsation, and operational reliability ( page 187).
All pumps are fitted with floating pistons, allowing compensation for small mechanical
tolerances within the specification and thus enhancing the robustness of the pump.
As a standard, all pumps are equipped with an active rear seal wash system ( page 21).
Various monitoring and diagnostic features are provided for optimum system
performance and reliability ( page 26).
The pump is designed for easy access to the fluid components, allowing fast and reliable
maintenance while the instrument remains in the UltiMate 3000 system stack.
For the secure and functional positioning of the solvent reservoirs on top of the pump, the
Solvent Racks of the UltiMate 3000 series are available from Dionex ( page 13).
Except for the SR-3000, all Solvent Racks include an integrated vacuum degasser.
The pump can be fully controlled by the Chromeleon Chromatography Management
System, providing a high degree of system integration.
All parts that may be exposed to solvents are made of materials that provide optimum
resistance to the most commonly used solvents and buffer solutions.
Operating Instructions Page 11
UltiMate 3000 Series:
SD, RS, and BM Pumps
2.2 Pump Configurations
2.2.1 Overview
The pump is available in the following configurations:
Pump Description Part No.
ISO-3100SD Isocratic pump (analytical, 1 solvent)
working pressure: up to 62 MPa (9000 psi)
ISO-3100BM Same as ISO-3100SD, however as
biocompatible micro pump with pulse damper ( page 24)
worki
ng pressure: up to 41 MPa (6000 psi)
LPG-3400SD Low-pressure gradient pump (analytical, 4 solvents)
with integrated vacuum degasser
working pressure: up to 62 MPa (9000 psi)
LPG-3400RS Same as LPG-3400SD, however,
working pressure: up to 103 MPa (15000 psi)
LPG-3400BM Same as LPG-3400SD,
however, as biocompatible micro pump
working pressure: up to 50 MPa (7250 psi)
DGP-3600SD Dual low-pressure gradient pump (analytical)
Two separate pumps in one enclosure (2 x 3 solvents),
working pressure: up to 62 MPa (9000 psi)
DGP-3600RS Same as DGP-3600SD, however,
working pressure: up to 103 MPa (15000 psi)
5040.0011
5042.0011
5040.0031
5040.0036
5042.0036
5040.0061
5040.0066
DGP-3600BM Same as DGP-3600SD,
however, as biocompatible micro pump
working pressure: up to 50 MPa (7250 psi)
HPG-3200SD High-pressure gradient pump (analytical; 2 solvents)
working pressure: up to 62 MPa (9000 psi)
HPG-3200RS Same as HPG-3200SD, however,
working pressure: up to 103 MPa (15000 psi)
HPG-3400SD High-pressure gradient pump (analytical) with
solvent selector (2 from 4)
working pressure: up to 62 MPa (9000 psi)
HPG-3400RS Same as HPG-3400SD, however,
working pressure: up to 103 MPa (15000 psi)
5042.0066
5040.0021
5040.0026
5040.0041
5040.0046
Page 12 Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
The isocratic pumps and the micro pumps have an inline filter installed. All other pumps
are shipped with a two-step mixing system, including a capillary mixer and a static mixer.
For more information, see page 22.
2.2.2 Combinations of UltiMate 3000 Pumps and Solvent Racks
The Solvent Racks of the UltiMate 3000 system series are an ideal complement to the
pumps of the UltiMate 3000 pump series, whether you need high efficiency degassing of
the solvents or simply want to safely organize your solvent reservoirs.
Solvent Rack Part No.
SRD-3200 Solvent Rack with analytical 2-channel vacuum degasser
typically for use with the following pumps:
- one HPG-3200
- one ISO-3100SD
SRD-3400 Solvent Rack with analytical 4-channel vacuum degasser
typically for use with the following pumps:
- one HPG-3400
- two HPG-3200s in a two-stack system
- one HPG-3200 or ISO-3100SD if you want to degas the solvents and the wash
liquid of an UltiMate 3000 series autosampler
SRD-3600 Solvent Rack with analytical 6-channel vacuum degasser
typically for use with the following pumps:
- one DGP-3600
- two HPG-3200s in a two-stack system
- one HPG-3200 and one HPG-3400 in a two-stack system
- one HPG-3400 if you want to degas the solvents and the wash liquid of an
UltiMate 3000 series autosampler
SR-3000 Solvent Rack without vacuum degasser
typically for use with an LPG-3400 or ISO-3100BM
5035.9250
5035.9245
5035.9230
5035.9200
For information about online degassing of the wash liquid, see the Autosampler Manual.
Operating Instructions Page 13
UltiMate 3000 Series:
SD, RS, and BM Pumps
2.2.3 Special Information for Biocompatible Pumps
The UltiMate 3000 pump series includes the following biocompatible pump types:
LPG-3400BM
DGP-3600BM
ISO-3100BM
Except for the fluid components, the BM pumps are identical to the SD and RS pumps.
Therefore, almost all descriptions in this manual apply also to the BM pumps. If some detail
applies to only one pump type, the type will be identified by name. The differences are as
follows:
The fluid components are made of titanium. Titanium is a base material, similar to aluminum
and magnesium. When titanium is processed, a titanium oxide film builds up on the
component surface, ensuring excellent corrosion resistance. The color of titanium and stainless
steel is slightly different. In addition, titanium parts are lighter than parts made of stainless
steel. Nevertheless, you can easily confuse titanium with stainless steel parts.
Observe the following:
When substituting parts be sure to install the appropriate replacement part for the BM
pumps. For information about the part numbers and for installation details, see the related
service sections ( page 123 and following pages) and the Consumables and Spare Parts
section ( page 199).
When connecting the solvent reservoirs, make sure to use appropriate filter frits on the
end of the solvent lines (shipped with the pump). As a standard, filter holders with
stainless steel frits are provided in the accessories kit of the SRD-3x00 Solvent Racks of
the UltiMate 3000 system. Replace the stainless steel frits with the frits from the
accessories kit of the pump ( page 48).
Ring seals with a titanium
spring are installed as piston seals in the biocompatible pumps,
whereas common ring seals with a steel spring are used in the SD and RS pumps.
Therefore, do not confuse these seals.
Page 14 Operating Instructions
UltiMate 3000 Series:
SD, RS, and BM Pumps
2.3 Operating Principle
The pump is a zero-pulsation, serial dual-piston pump with electronic compressibility
compensation. The pump head includes two cylinders—working cylinder and equilibration
cylinder—that are connected in series. The solvent passes both cylinders successively.
Continuous delivery is achieved as follows: The working cylinder delivers at the
appropriate flow rate while simultaneously filling the serially connected equilibration
cylinder. The latter serves as a reservoir and delivers while the working cylinder carries
out the suction stroke.
The characteristic feature of the patented isokinetic pre-compression is the overlapping phase
of the delivery strokes of the working and equilibration cylinders. When delivering
compressible liquids without controlled pre-compression, the pulsation increases as the
working pressure increases, since part of the delivery stroke is required for compressing the
solvent in the working cylinder. Pulsation during the pre-compression phase is reduced to a
minimum by velocity modulation of the drive. The highly constant delivery is ensured by a
patented secondary control system (automatic compressibility compensation). The flow rate is
always kept constant in relation to the atmospheric pressure.
The schematics in section 8 illustrate how the pumps operate ( page 161).
Operating Instructions Page 15
UltiMate 3000 Series:
SD, RS, and BM Pumps
2.4 Front Panel Elements
3
2
1
Fig. 1 Front panel view
No. Front Panel
Element
1 Display Shows information about the pump, for example:
2 Standby button Switches the pump to Standby mode (the LED is red).
3
LEDs
Power The LED is blue when the pump is turned on.
Connected The LED is green when the pump is connected in Chromeleon.
Status The LED is green when the pump is ready for operation.
Description
- General information upon power up ( page 61)
-
Status screen ( page 62)
-
Functions and menus ( pages 70 and 72)
- Messages
To cancel Standby mode and resume operation, press the Standby button
again (the LED is not lighted).
Notes:
To allow the pump to change the mode, press and hold the Standby button for
at least 1 second. If you switch a pump to which an SRD-3x00 Solvent Rack
is connected to the Standby mode, the Solvent Rack, too, will be set to
Standby mode.
The LED is red when an error has been detected, for example, a leak.
( page 105)
Page 16 Operating Instructions
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