Dionex RF-2000 Operating Instructions Manual
Fluorescence Detector
RF-2000
Operating Instructions
Version: 1.40
Datum: July 7, 2000
© 2000 Dionex Softron GmbH
Doc.: RF2000_OI_E_V140.doc
Operating Instructions RF-2000
DECLARATION OF CONFORMITY
We, Dionex Softron GmbH, declare that the product Fluorescence Detector
RF-2000
to which this declaration relates, is in conformity with the following European standards:
# EN 50081-1 (1992):
# EN 50082-1 (1992):
Attention
January 27, 1998 QM-Manager
Dionex Softron will not accept any liability for damage, direct or indirect,
caused by connecting this instrument to devices which do not meet relevant
safety standards.
Electromagnetic compatibility - Generic emission standard
Part 1: Residential, commercial and light industry
Electromagnetic compatibility - Generic immunity standard
Part 1: Residential, commercial and light industry
Operating Instructions RF-2000
CONTENTS
1 Precautions ................................................................................................3
1.1 Warnings in these Operating Instructions ................................................................ 3
1.2 Warning Labels on the Unit ..................................................................................... 3
1.3 Replacement of Fuses............................................................................................. 5
1.4 Precautions on Installation Site and Handling of the Unit......................................... 6
1.5 Precaution in the region where 240V is used as working voltage............................. 7
1.6 Precautions on Static Electricity............................................................................... 8
2 General Information................................................................................. 11
2.1 Outline................................................................................................................... 11
2.2 Features................................................................................................................ 11
3 Component Location and Function .......................................................12
3.1 Front View............................................................................................................. 12
3.2 Top Side and Right Side........................................................................................ 13
3.3 Bottom Side........................................................................................................... 13
3.4 Rear View.............................................................................................................. 14
4 Installation ................................................................................................15
4.1 Basic Installation Requirements............................................................................. 15
4.2 Electrical Connections........................................................................................... 16
4.3 Fluidic Connections ............................................................................................... 17
4.4 Connecting the Recorder and the Integrator.......................................................... 18
4.5 Connecting a Drain Tube for Solvent Leakage ...................................................... 19
4.6 Installing the Xenon Lamp ..................................................................................... 19
4.7 Adjusting the Xenon Lamp Alignment.................................................................... 23
5 Operation ..................................................................................................26
5.1 Precautions for Operation...................................................................................... 26
5.2 Fundamentals of Operation ................................................................................... 26
5.3 Operating Procedure in Spectrum Scanning Mode ................................................ 33
5.4 Creating and Executing a Time Program ............................................................... 36
5.5 Additional Functions .............................................................................................. 42
6 Performance Verification ........................................................................ 50
6.1 Outline of System Validation.................................................................................. 51
6.2 Repeatability Test of Chromatographic Data (Isocratic LC) ................................... 51
6.3 Repeatability Test of Chromatographic Data (Gradient LC) ................................... 53
7 Control from External Equipment ..........................................................56
7.1 Connecting External Input and Output Terminals................................................... 56
7.2 Control from External Equipment via RS-232C...................................................... 58
i
Operating Instructions RF-2000
8 Maintenance ............................................................................................. 64
8.1 Maintenance of Flow Cell ...................................................................................... 64
8.2 Checking the Xenon Lamp .................................................................................... 70
8.3 Replacing the Fuse................................................................................................ 72
8.4 Changing the Set Power Supply Voltage ............................................................... 73
8.5 Periodical Cleaning................................................................................................ 74
9 Troubleshooting ...................................................................................... 75
9.1 Summary of the Most Frequently Problems ........................................................... 75
9.2 List of Error Messages........................................................................................... 80
10 Specifications ....................................................................................... 84
11 Standard Accessories ..........................................................................86
12 Replacement Parts ...............................................................................86
ii
Operating Instructions RF-2000
1 Precautions
The Dionex RF-2000 is the fluorescence detector for the high performance liquid
chromatograph. In order to operate the unit safely, strictly observe the following
points. It is dangerous not to comply with the following points.
• Use the unit for any purpose mentioned above.
• Follow the procedures described in the operating instructions.
• Observe the warnings and cautions.
• Do not disassemble or modify the unit without approval from Dionex. Failing to do
so may lead to a dangerous situation or damage to the instrument.
• For internal repair of the product, contact the Dionex Service .
1.1 Warnings in these Operating Instructions
Warning:
Important:
Please note:
Indicates that failure to take note of the accompanying
information may result in personal injury.
Indicates that failure to take note of the accompanying
information may cause wrong results or damage to the instrument
Indicates General information to assist you in obtaining optimum
performance.
1.2 Warning Labels on the Unit
For safety of operation, this unit is provided with the mark at the portion where
special cautions are required. When operating the parts where this mark is indicated,
exercise special caution after reading the operating instructions.
Warning:
• The xenon lamp may explode in the course of long use (more than 1000hours).
Replace the lamp of expired life (500 hours) with a new one.
• Be sure to wear protective gears, such as protective mask, heavy and longsleeved shirt and gloves, when handling the xenon lamp. Since high pressure gas
is charged in the xenon lamp, if the lamp is strongly shocked or the glass surface
is scratched, the lamp may explode and pieces of glass may scatter.
• When mounting or replacing the xenon lamp, be sure to turn OFF the power
switch and disconnect the power cable. Voltage is applied to the electrode by the
electric charge right after the power switch is turned OFF. Therefore, leave the
xenon lamp for more than five minutes after turning the power switch OFF before
opening the cover of the light source chamber.
• The xenon lamp is very hot when it is lit. Therefore, when replacing the xenon
lamp, wait for more than an hour after turning the power OFF to cool the lamp
enough for replacement.
• High voltage (approx. 30 kV) is applied to the xenon lamp during ignition. When
turning ON the power, be sure to mount the lamp and close the cover of the light
source chamber.
3
Important:
Be sure to read this instruction manual before supplying power to this unit.
• Be sure to mount the xenon lamp correctly before supplying the power to this unit
(refer to 4.7 Installing the Xenon Lamp). Failing to do so may damage the main
unit because high voltage around 30kV is applied to electrode when turning ON
the xenon lamp.
• Use the power supply voltage at the state which conforms to the power supply
setting (display of the power supply connector unit at the rear) of this unit. For
changing the power supply setting of this unit, refer to 8.4 Changing the Set Power
Supply Voltage. However, when the voltage is 220V ± 10%, set the power supply
voltage of this unit at 230Vac.
Operating Instructions RF-2000
4
Operating Instructions RF-2000
1.3 Replacement of Fuses
This unit uses the following fuse. Be sure to replace the fuse of the same type and
capacity.
Power supply voltage: 220-240 V
Part no. 706.072.0165221
250V 3.15AT
Important:
When the xenon lamp is lit, the flow cell is irradiated by the
excitation light beam. Do not look into the excitation light beam,
while the xenon lamp is lit.
5
Operating Instructions RF-2000
1.4 Precautions on Installation Site and Handling of the Unit
Generally, a large amount of organic solvents is used in the high-performance liquid
chromatograph. Sufficient care should be taken in installation site and handling of the
unit. Please take care of the notices in the text, not to mention the precautionary
requirements listed below:
1.4.1 Ventilation
Solvents used in the high-performance liquid chromatograph are inflammable
and/or toxic. Be sure to ventilate the room well.
1.4.2 Fire
The lighting of fires is prohibited in the room where high-performance liquid
chromatograph is installed. Do not install any devices which may emit sparks
in the room. It is also necessary to provide a fire extinguisher preparing for the
worst.
1.4.3 Safety goggles
Wear safety goggles in handling solvents.
1.4.4 Other equipment
A washstand or the like is necessary to be furnished nearby preparing for the
cases such that solvent comes into the personnel's eyes or the personnel
touches toxic solvent.
1.4.5 Power source
Working voltage range and power consumption of this system are as follows.
Be sure to connect to the power source conforming to them.
Part No. 706.228.35353.38, 220-230V ~ ±10%/240V ~ ±10%, 350VA,
50-60Hz
If working with a different working range, different fuses must be used! Please
contact the Dionex Service.
1.4.6 Grounding
To prevent electric shock and to secure safe operation of the system, always
make grounding.
1.4.7 Repair and maintenance of the unit
Normal maintenance of this unit can be performed without removing the
cover. Do not remove the cover at the normal maintenance. Contact the
Dionex Service if repair requires to remove the cover of the main body.
6
Operating Instructions RF-2000
1.5 Precaution in the region where 240V is used as working
voltage
1.5.1 Confirming the working voltage
The voltage setting must correspond to the working voltage used. When the working
voltage is between 220V and 230V, it is not necessary to change the factory setting
(230Vac). However, when the working voltage is 240V, change the voltage setting as
follows:
(1) Turn OFF the power switch.
(2) Pull out the power supply cable from the power supply connector.
(3) Open the fuse holder cover at the rear of this unit by a slotted screwdriver.
(4) Change the rotary switch showing the voltage in the fuse holder to display
240Vac.
(5) Close the fuse holder cover until a clicking sound is heard.
7
Operating Instructions RF-2000
1.6 Precautions on Static Electricity
Liquid chromatography using flammable organic solvents as mobile phase requires
proper care against fire, explosion, etc. Particularly, among various possible
accidents, those caused by static electricity are difficult to anticipate, and tend to
occur only with unexpected conditions which often make countermeasures
insufficient. At a site where preparative liquid chromatography is practiced, a large
amount of flammable substances may be used. Therefore, once an accident
happens, it could lead to tremendous damage. The mechanism of accident caused
by static electrical discharge and preventive measures are described below. Take
due care in safety measures in handling of equipment.
1.6.1 Mechanism of Static Electrical Discharge Accident (Example)
Accidents caused by static electricity take place through the following processes.
When liquid is fed at high speed through a small-diameter tube like the pipe of a
liquid chromatograph, static electrical charge occurs by friction between solid and
liquid as shown in the figure below
Fig.: Occurrence of Static Electricity by Friction between Solid and Liquid
When the charged liquid is collected in an insulated vessel, the static charge
accumulates gradually, and the voltage can easily reach a few kilovolts. If some other
conductive object is brought near the vessel, electricity is discharged at a certain
distance from the vessel releasing heat energy. If flammable gas of sufficient
concentration exists nearby, ignition is caused by this energy.
8
Operating Instructions RF-2000
Fig.: Conditions which may cause accidents
1.6.2 Preventive Measures against Accidents
The principal preventive measure is the prevention of charging and storage of static
electricity among those items shown in Mechanism of Static Electrical Discharge
Accidents. The preventive measures are shown below. It is recommended to
exercise two or more measures simultaneously.
• Particularly when a large quantity of flammable solvent is held in a large vessel,
be sure to observe the preventive measures 1, 2, and 3.
Preventive measure 1.
Use metallic (conductive) waste liquid vessel which is well grounded. This releases
the charge of the waste liquid and vessel to ground.
• Be sure to ground the vessel properly. Disconnecting of grounding wire or poor
grounding defeat the purpose of using a metallic vessel.
• There are some metallic cans which have no conductivity due to an oxidized
coating or lacquer on their surface. Be sure to confirm the grounding of vessels by
a tester before application.
• When a liquid with almost no conductivity (of 10-10s/m or less) is discharged into
the vessel, it is necessary to mix it with another liquid with some conductivity. (The
other liquid can be placed in the vessel in advance.)
Preventive measure 2.
Minimize the clearance of both inlet and outlet of vessel to prevent flame from
entering the vessel.
• Use a cap with three holes for 18 liter and 4 liter cans.
9
Operating Instructions RF-2000
Fig.: Anti-static electricity measures for vessel
Preventive measure 3.
Do not approach the vessel with charged objects including the human body.
Charging prevention measures for human body
a) Prevention of charging of shoes and clothes
b) Grounding of human body
c) Make working floor conductive
Suitable products to be used for those measures a), b), and c) are available on the
market.
• When persons who use no charge prevention measures approach dangerous
sections, they have to be grounded beforehand. (For example, they should
contact grounded metal by hand.)
Preventive measure 4.
Use pipes with inner diameter of 2mm or more for waste liquid line for large flow
rates.
• Inclusion of bubbles in the tube may increase the amount of charging by ten times.
Check that there is no inclusion of air via tube joints.
Preventive measure 5.
When it is impossible to use a conductive vessel, use caution in the following points.
a) Set the vessel so that the pipe outlet will be placed below the liquid level in
the vessel. Or, dip a grounded metal (ex. pipe connected to the main body of
device) in the liquid. Please note: This method is not effective for liquid with
small conductivity (10-10s/m or less).
b) Use a vessel of the smallest possible capacity to minimize the damage by fire
if it should occur.
c) Prevent the room from being dry. Humidity of 65% or more has charge
prevention effects.
10
Operating Instructions RF-2000
2 General Information
2.1 Outline
The basic design concept of the Dionex Fluorescence Detector RF-2000 is to
enhance productivity of analysis through its highest performance and stability.
This concept is realized by the highest sensitivity of the detector (higher than 300
signal to noise ratio for Raman spectrum of water), the highest wavelength accuracy
(± 2nm), the highest wavelength reproducibility (± 0.2nm) and the GLP/GMP
compliance support and improvement of operational safety.
The Dionex RF-2000 supports operation via the data system CHROMELEON.
Please refer to the sections "7.2 Control from External Equipment via RS-232C" and
"7.2.3 Controlling the RF-2000 via CHROMELEON" for details.
2.2 Features
• Greater sensitivity
The reengineered RF-2000 optical system provides significantly increased
sensitivity (signal-to-noise ratio greater than 300 for Raman spectrum of water).
Furthermore, use of appropriate excitation and emission spectral scan conditions
together with wavelength time programming allow high sensitivity detection of
multiple components at trace level concentrations.
• Enhanced wavelength accuracy
The RF-2000 offers unsurpassed wavelength accuracy (± 2nm) and wavelength
reproducibility to further enhance the reliability of wavelength selectivity and unitto-unit performance.
• Support for GLP/GMP compliance
Daily checks and log-keeping can be automated*. The standard validation
documents such as Certificate of Compliance and Hardware Validation SOP are
provided to facilitate compliance with regulatory requirements.
*With optional System Check software
• Enhanced safety features
Both heat and leak sensors are provided as a standard feature. In addition, the
lamp may be turned ON/OFF via time program, minimizing unnecessary lamp
consumption during continuous, unattended runs.
11
Operating Instructions RF-2000
3 Component Location and Function
3.1 Front View
No. Description Function
1 Control panel Performs setting and display.
12
3.2 Top Side and Right Side
Operating Instructions RF-2000
No. Description Function
1 Light source chamber cover Remove this cover when replacing the
lamp.
2 Cell cover Remove this cover when replacing the
cell.
3 Cell inlet tubing Marked with blue cover tubing.
4 Cell outlet tubing
3.3 Bottom Side
No. Description Function
1 Drain tube for solvent leakage Drain tube is connected here.
13
3.4 Rear View
Operating Instructions RF-2000
No. Description Function
1 Horizontal direction adjusting
screw
2/upper Vertical direction adjusting screw Adjusts vertical alignment of the lamp on
2/lower Lamp fixing screw Used to fix the lamp.
3 Fan Removes internal heat.
4 External input and output
terminals
5 Grounding terminal Grounding is made through this terminal.
6 Fuse holder Fuse is set in this holder.
7 Power supply connector Connector for power cable
8 RS-232C connector Connector for control by CHROMELEON
Adjusts horizontal alignment of the lamp
on replacement.
replacement.
Terminals used for connection with
external instruments.
14
4 Installation
4.1 Basic Installation Requirements
Operating Instructions RF-2000
Warning:
1. Ventilation Ventilate the room where the high performance liquid
2. Fire Never use fire in the same room where the high
3. Sink Install a sink nearby for flushing eyes or skin which have
4. Corrosive gas and
dust
5. Electromagnetic noise Avoid locations subject to intense magnetic or
6. Space requirements This system is designed to be used on a table or stand,
7. Others Select an installation site with the following parameters to
(1) Maintain room temperature within 4~35C, without
(2) Avoid direct output of a heater or a cooler.
(3) Avoid exposure to direct sunlight.
(4) Avoid locations subject to strong vibrations or
(5) Maintain relative humidity within 45~85%.
(However, humidity should be below 70% when the
To take full advantage of the RF-2000 performance capabilities and
to ensure its operational stability over a long service life, verify that
the selected installation site satisfies the following requirements.
chromatograph is located since the solvent used is
flammable and/or toxic.
performance liquid chromatograph is installed. Also, avoid
installation in the same room of other devices which may
spark. Always keep a fire extinguisher nearby in case of
accident.
been in contact with solvent.
Avoid installation in a place exposed to corrosive gases or
dust.
electromagnetic fields. Use an additional noise filter if
power line noise interferes.
preferably a solid and flat surface with a depth of 70cm or
more.
maintain full performance of the system.
extreme fluctuations.
prolonged weak vibrations.
room temperature is 30C or higher.)
15
4.2 Electrical Connections
Operating Instructions RF-2000
Warning:
When the power supply voltage is 220-230V~, operate the unit with
the voltage setting of the power supply connector at the rear of this
unit at 230Vac. When the power supply voltage is at 240V~, set the
power supply connector at 240Vac. For changing the setting of
power supply connector, refer to "8.4 Changing the set power
supply voltage".
If the power supply is not stable or the capacity insufficient,
satisfactory performance is not possible. Verify for the total power
supply for the system before preparing power supply.
• Verify that the power switch of the main unit is turned OFF.
4.2.1 Connection to outlet
(1) Connect the female connector of the power supply cord supplied with the unit
to the power supply cord connector at the rear of the unit, and plug the male
connector into a power supply outlet.
(2) Supplied power supply cord is of three-conductor (3P) type.
When connecting to two-conductor (2P) type power supply outlet, use the
provided power supply adaptor.
4.2.2 Grounding
(1) When three-conductor (3P) type power supply outlet is used, the unit is
grounded by the power supply cord.
(2) When two-conductor (2P) type power supply outlet is used, the unit is not
grounded. In this case, carry out grounding from the grounding terminal on
the unit rear panel.
To prevent electric shock and to secure safe operation of the system, always
ground the unit.
16
Operating Instructions RF-2000
4.3 Fluidic Connections
4.3.1 Connecting the Detector and the Column
Typical connection between this equipment and the column is described in this
section.
(1) Cut the piping tube (SUS Tube 1.6 x 0.3, standard accessory) at the length
necessary for connecting the column outlet and the cell inlet pipe (pipe with
blue tape) of this equipment.
Cut the tube perpendicular. If the tube is cut diagonally, dead volume is
generated to deteriorate separation.
(2) Connect the piping between the column and the cell inlet pipe with the
supplied male PEEK nut and the male to male union as shown in the figure
below. Tighten the male PEEK nut by the hand firmly. It is not necessary to
use a tool for tightening.
Please note:
Insert the piping tube into the column joint and the male to male
union securely until it comes to the end and then tighten the
male PEEK nut. Similarly, insert the cell inlet pipe until it comes
to the end and tighten the male PEEK nut so that dead volume
is avoided.
4.3.2 Detector Outlet Side Piping
(1) Prepare a waste liquid bottle.
(2) Connect the male to male union and the Tefzel tube (standard accessories of
this product) to the cell outlet pipe and put the other end in the waste liquid
bottle, as shown in the figure.
Please note:
Pressure tolerance of the cell is 1960 kPa {20kgf/cm2} (Value in
{} is for reference). Pressure exceeding this level may cause
leak of liquid or damage of the cell. To prevent such troubles,
pay attention to the following points:
17
• Do not apply excessive back pressure to the outlet side of the cell.
• In order to prevent clogging in the flow line, thoroughly clean the flow line before
stop delivery of solvent when the analysis is finished. Especially, when the buffer
solution is used, salts may be crystallized inside flowline. Be sure to clean the flow
line.
• When delivery of the solvent is stopped temporarily, put the tip of the flow line
outlet in the waste liquid in the bottle to prevent clogging of the flow line. This is to
keep inside the flowline being wet.
Operating Instructions RF-2000
Important:
When a mobile phase with high insulation, such as hexane,
is used, it is dangerous since static electricity is changed
at the waste liquid bottle. Use a metal liquid bottle for
waste and ground the bottle.
(Refer to section "1.6 Precautions on Static Electricity")
4.4 Connecting the Recorder and the Integrator
4.4.1 Connecting with a Recorder
Connect the RECORDER terminal of this instrument and the recorder using the
provided signal cable.
When using a stranded wire, twist the end tightly, or tin it with solder. Using a small
screwdriver or other tool, depress the rectangular button adjacent to the appropriate
terminal hole. Insert the wire and release the button to clamp the wire in position, as
shown in the figure below.
Important:
Be sure to turn the power of the main unit OFF before
connecting the signal cable.
4.4.2 Connecting with an Integrator
Connect the integrator to the INTEGRATOR terminals in the external input/output
terminals at the rear of the RF-2000. Connect the cable supplied with the integrator.
Connect each terminal of the signal cable to the terminal block supplied with the
integrator.
18
Operating Instructions RF-2000
4.5 Connecting a Drain Tube for Solvent Leakage
Each component in the RF-2000 is designed so that liquid leakage in the equipment
is discharged from the liquid leak tube connection port at the right side or the lower
front side of the equipment. Connect liquid leak tube if necessary.
4.5.1 Connecting a Liquid Leak Tube
Cut the drain tube (standard accessory) for liquid leakage at an appropriate length
and connect the waste liquid tube to the drain tube and put the other end in the waste
liquid bottle.
Important:
Place the waste liquid bottle lower than the device at the
bottom. If the flow line of the liquid leak discharging tube is
placed above the bottom face of the RF-2000, the waste liquid
cannot be drained.
4.6 Installing the Xenon Lamp
Be certain that the AC power cable plug is disconnected from this equipment for
prevention of electric shock when installing the xenon lamp.
Warning:
• Be sure to wear protective gears, such as protective mask,
heavy and long sleeve shirt and gloves, when handling the
xenon lamp.
• Since high pressure gas in changed in the xenon lamp, if the
lamp is strongly shocked or the glass surface is scratched,
the lamp may explode and piece of glass may scatter.
19
Operating Instructions RF-2000
Warning:
• When the light source chamber cover shall be opened, be
sure to turn OFF the power switch and disconnect the power
cable from power supply.
• It is very dangerous to touch the lamp by any mistake
because high voltage, approx. 30kV, is applied to the + pole
of the xenon lamp when the lamp is lit on and 20V is applied
while the lamp is lit.
• The xenon lamp is still charged just after the power switch is
turned OFF. Leave more than 5 minutes after the power
switch is turned OFF before open the light source chamber
cover to avoid electric shocks.
• It is very high temperature right after the Xenon lamp is
turned OFF. Wait until it is cooled down sufficiently for
replacement. The time required for cooling down the lamp is
an hour when it is left with its power supply turned OFF,
while it is 30 minutes when the lamp is turned OFF with
"LAMP" of the AUX FUNC set at "0".
4.6.1 Installation procedure
(1) Remove the two mounting screws of the light source chamber cover with a
screwdriver and remove the light source chamber cover.
(2) Loosen the sliding plate screws and slide the sliding plate so that the inside of
the light source chamber case can be seen.
(3) Insert the supplied screwdriver in the hole for screwdriver for lamp fixing along
the guide and turn in counterclockwise to loosen the lamp fixing screw.
(4) Take the xenon lamp out of the case and remove the knurled screws at the
plus pole and the minus pole of the lamp.
(5) Turn the plus pole of Xenon lamp upward, and insert the xenon lamp into the
xenon lamp holder with the gas filling protrusion (tip off) of the xenon lamp
faced to the opposite side of the beam-condensing mirror.
20
Important:
When doing this, do not touch the valve surface of the lamp. If
Operating Instructions RF-2000
touched, wipe off the surface with a gauze wet with ethanol
(provided with the lamp), etc. If the lamp is lit with fingerprints
on it, the fingerprints are burned and causes explosion of the
lamp valve.
(6) Tighten the lamp mounting screw with the screwdriver through the hole for
screwdriver for lamp fixing to fix the lamp.
(7) Close the sliding plate and fix it with the sliding plate screws.
21
(8) Set the terminal of cable connected to the plus pole in the screw at the plus
pole of the lamp and fix it with a knurled screw securely.
(9) Remount the light source chamber cover.
Operating Instructions RF-2000
Warning:
When tightening the terminal at the plus pole with a
knurled screw, be sure to allow the cable to the plus pole
be bent.
If the plus pole is stretched by the cable, there occurs a
danger that the xenon lamp may be destroyed while being
lit. Besides, when tightening the knurled screw, be sure to
tighten by fingers. It is dangerous to use any tool such as
wrench.
22
Operating Instructions RF-2000
4.7 Adjusting the Xenon Lamp Alignment
4.7.1 Adjusting the light source position (coarse adjustment)
Adjust the light source position for maximum equipment performance. Be sure to
adjust the light source position during installation or replacement of the lamp.
Adjustment consists of coarse and fine adjustment.
Please note:
(1) Turning power ON, and make sure lamp LED on the front panel is lit.
(2) Set excitation wavelength to 540nm.
(3) Remove the flow cell and place a piece of white paper where the flow cell was
installed and where green light is observed on the paper.
(4) Adjust the light source horizontal direction adjusting screw for maximum
brightness of the green light on the paper.
(5) Adjust the light source vertical direction adjusting screw in a same way as
above (4).
(6) Remove the paper.
The xenon lamp may not be lit on if the lamp is heated just after
lit off after being lit on for a while. In such case, wait for about
10 minutes until the lamp is cooled and turn ON the power
switch again.
23
Operating Instructions RF-2000
4.7.2 Adjusting the light source position (fine adjustment)
Important:
(1) Connect a recorder to the 10mV RECORDER terminal of the RF-2000 (see
section 7.1), and turn the power ON.
(2) Remove the flow cell from the RF-2000. Pour distilled water into the flow cell
using the provided syringe. Make sure that no bubbles are present in the flow
cell, and install the flow cell in the sample compartment (see "8.1
Maintenance of Flow Cell").
(3) Set recorder parameter as follows:
1) Range : 10mV/Full Scale
2) Chart Speed : 10mm/min
(4) Adjust the zero position by pressing the zero key of the RF-2000.
(5) Adjust the zero position of the recorder.
(6) Press func key of the RF-2000 until the fluorescent character displays
(7) Press ENTER.
The parameters are automatically set as follows
(Refer to "5 Operation"):
1) EX : 350nm
2) EM : 450nm
3) GAIN : 2 ( x 4)
4) RANGE : 4 ( x 1/8)
5) RATIO : 0 (OFF)
6) sens : 2 (MED)
7) rsp : 1 (0.1s)
8) Z WAVE : 1 (OFF)
The above parameters are set and the auto-zero function is activated
automatically while the following message appears on the screen.
If a pen recorder is connected, proceed as described below. If
not, please contact the Dionex Service for the fine adjustment of
the light source position.
LAMP ADJUST
Then the emission wavelength is changed and set at 397nm automatically
and the following message appears.
Please note:
SETTING ZERO
LAMP ADJUST XX.XX
Be sure to obey the order of adjustment of the horizontal
direction adjusting screw and the vertical direction adjustment
screw as described below. If the order is not correct, the light
source position deviates greatly from the slit of the excitation
monochromator and re-adjustment cannot be possible. In this
case, carry out the adjustment from "4.7.1 Adjusting the light
source position (Coarse adjustment)" again.
24
(8) Adjust the horizontal direction adjusting screw on the rear panel, till the pen
position comes to the maximum point. (till the value in the display indicates its
maximum.)
(9) Adjust the vertical direction adjusting screw on the rear panel till the pen
position comes to the maximum point. (till the value in the display indicates its
maximum.)
(10) Repeat the steps (8) and (9) so that the maximum value at the horizontal
direction adjustment and that of the vertical direction adjustment become the
same. In many cases, the value indicated on display of RF-2000 is more than
30.
(11) Stop recording by the recorder. To stop, press shift down, plot and Enter.
(12) Pressing the CE key resets each parameter at the state before "LAMP
ADJUST" is performed.
Operating Instructions RF-2000
25
Loading...