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This Quick Installation Guide explains the basic installation steps for a P680 ISO isocratic
pump system. For additional information, please consult the Operating Instructions for the
P680 HPLC Pumps.
1. Preparation
Bring the pump to a moderate temperature for four hours to allow any condensation that
might have occurred during transit to evaporate. Do not connect the pump to the mains during
this period. If it is obvious that condensation did not evaporate completely, continue to warmup the pump (which is not connected to the mains) until the condensation is completely gone.
Place the pump on a firm and level surface that is free of vibration. Make sure that the surface
is resistant to solvents. Avoid locations with extreme changes in temperature (such as direct
sunlight or drafts) and high air humidity.
2. Remove the caution label
You may remove the “caution” label
from the pump's enclosure.
Important: The pump is primed with 2-propanol. During initial operation of the
pump, make sure that the solvents used are miscible with
2-propanol. Otherwise, follow the appropriate intermediate steps.
3. Connect the eluent tube to the solvent bottle
The bottle caps have five holes. Four holes are capped by default (white caps), one hole is
open for the eluent tube. A retaining guide holds the tubing in place.
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Guide the eluent tube through the retaining
guide and into one of the five holes in the
bottle cap.
Retaining guide
Place the eluent frit on the end of the tube,
place the complete assembly into the bottle,
and hand-tighten the bottle cap.
Please note: To exchange the tubes, remove the frit first , then the eluent tube, and then
the retaining guide.
Important: To draw solvents from the solvent bottles, use only eluent tubes with filter
frits. This prevents contamination of the HPLC system.
Please note: Regularly check the frits for permeability. Especially when working with
aqueous solvents, algae and other microorganisms can grow on the frits
and clog them. Therefore, exchange the solvents at regular intervals. Rinse
the bottles thoroughly before using them again. Replace the frits as
necessary.
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4. Place the SOR-100 Solvent Rack on top of the
pump
The SOR-100A-2 Solvent Rack (part no.
5030.9210) fits on top of the P680.
For safety reasons, place the eluent
bottles on the tray on top of the pump.
The SOR-100A-2 Solvent Rack is shipped with one 1-liter eluent bottle, one 2-liter eluent
bottle, appropriate tubing, and bottle caps.
5. Connect the SOR-100 with the P680
Please note: The pictures below show a 6-channel degasser. For a P680A ISO, an SOR-100-2
(part no. 5030.9210) with an integrated 2-channel degasser is sufficient.
The SOR-100 fits on top of the P680. For
safety reasons, always place the eluent bottles
on the rack, on top of the pump. Open the
SOR-100 by tilting the front panel upward.
Pull the tray with the degassing chambers
toward you.
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© 2004 Dionex