Dionex ICS-2100 Installation Instructions Manual

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ICS-2100 Ion Chromatography System

Installation Instructions

Document No. 065294

Revision 01 March 2009

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This publication is protected by federal copyright law. No part of this publication may be copied or distributed, transmitted, transcribed, stored in a retrieval system, or transmitted into any human or computer language, in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties without the express written permission of Dionex Corporation, 1228 Titan Way, Sunnyvale, California 94088-3603 U.S.A.

DISCLAIMER OF WARRANTY AND LIMITED WARRANTY

THIS PUBLICATION IS PROVIDED “AS IS” WITHOUT WARRANTY OF ANY KIND. DIONEX CORPORATION DOES NOT WARRANT, GUARANTEE, OR MAKE ANY EXPRESS OR IMPLIED REPRESENTATIONS REGARDING THE USE, OR THE RESULTS OF THE USE, OF THIS PUBLICA TION IN TERMS OF CORRECTNESS, ACCURACY, RELIABILITY, CURRENTNESS, OR OTHERWISE. FURTHER, DIONEX CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVISION OR CHANGES.

TRADEMARKS

AES, Atlas, Chromeleon, EluGen, and SRS are registered trademarks of Dionex Corporation. Microsoft, Windows, and Windows Vista are registered trademarks of Microsoft Corporation. PEEK is a trademark of Victrex PLC.

PRINTING HISTORY

Revision 01, March 2009

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1. Facility Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

2. Unpacking the IC System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

3. Connecting an Autosampler (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . 4

3.1 Connecting an AS-DV Autosampler . . . . . . . . . . . . . . . . . . . . . 4

3.2 Connecting an AS Autosampler . . . . . . . . . . . . . . . . . . . . . . . . . 5

4. Unpacking the PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

5. Installing Chromeleon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

6. Installing the Chromeleon Software License Dongle . . . . . . . . . . . . . . 10

7. Installing the Software License . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

8. Starting the Chromeleon Server . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

9. Connecting to the Chromeleon PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

9.1 Connecting the IC System to the PC . . . . . . . . . . . . . . . . . . . . 13

9.2 Connecting Additional USB Devices . . . . . . . . . . . . . . . . . . . . 14

10. Connecting the Power Cord . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

11. Installing the ICS-2100 Device Driver . . . . . . . . . . . . . . . . . . . . . . . . . 17

12. Setting Up Chromeleon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

12.1 Creating a Timebase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

12.2 Configuring the Device Properties . . . . . . . . . . . . . . . . . . . . . . 21

12.3 Connecting to the Panel Tabset . . . . . . . . . . . . . . . . . . . . . . . . 24

12.4 Verifying Chromeleon Communication . . . . . . . . . . . . . . . . . . 25

13. Installing and Plumbing the Columns and Suppressor . . . . . . . . . . . . . 26

13.1 Unpacking the Columns and Suppressor . . . . . . . . . . . . . . . . . 27

13.2 Setting Up the Column Heater . . . . . . . . . . . . . . . . . . . . . . . . 27

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ICS-2100 Installation Instructions

13.3 Plumbing the Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29

13.4 Installing the Suppressor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

14. Connecting the Waste Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31

14.1 Installing the Waste, Gas Separator Tube . . . . . . . . . . . . . . . . .32

15. Setting Up the Eluent Reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

16. Entering the Eluent Level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

17. Priming the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

17.1 Priming the Eluent Lines with a Syringe . . . . . . . . . . . . . . . . .35

17.2 Priming the Pump Heads with the Prime Button . . . . . . . . . . . .37

18. Setting Up the Eluent Generator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

18.1 Eluent Generator Setup for KOH, LiOH, MSA, and

NaOH Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

18.2 Eluent Generator Setup for Carbonate/Bicarbonate Eluent . . . .54

18.3 Eluent Generator Setup for Carbonate Eluent . . . . . . . . . . . . . .80

19. Equilibrating the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97

19.1 EluGen Cartridge Backpressure Requirements . . . . . . . . . . . . .97

20. Verifying Operational Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .98

21. Connecting the Analog Output (Optional) . . . . . . . . . . . . . . . . . . . . . .100

22. Pressurizing the Eluent Reservoir (Optional) . . . . . . . . . . . . . . . . . . . .100

22.1 Connecting the Gas Source . . . . . . . . . . . . . . . . . . . . . . . . . . .101

22.2 Applying the Pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 02

23. Connecting the Pump Continuous Seal Wash (Optional) . . . . . . . . . .103

24. Sample Processing Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105

25. Troubleshooting the USB Configuration Error . . . . . . . . . . . . . . . . . .106

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Installation

This document provides instructions for the initial installation of the ICS-2100 Ion Chromatography System

NOTE The order of the steps is important. It is especially

important to install the Chromeleon Management System before connecting the USB cable and turning on the system power.

1. Facility Requirements

• Make sure the installation site meets the following environmental

specifications: Main Power: 100 to 240 Vac, 50 to 60 Hz (Auto-sensing power supply; no

manual voltage or frequency adjustment required)

Operating Temperature: 4 to 40 °C (40 to 104 °F Humidity: 5% to 95% relative humidity, noncondensing

• Provide a sturdy workbench for the system of a height that ensures convenient

access to the interior of the ICS-2100.

• Allow at least 15 cm (6 in) behind the system for power connections and

ventilation. For optimal performance, install the system in a draft-free location, out of the path of air conditioning and heating vents.

• (Optional) For pressurization of the eluent reservoir, provide a clean gas

source regulated to between 0.55 and 0.83 MPa (80 and 120 psi). An air regulator accessory (P/N 060054) is required to regulate the pressure to between 0.03 and 0.04 MPa (5 and 6 psi).

Chromatography

)

Do not pressurize the eluent reservoir above 0.07 MPa (10 psi).

Ne mettez jamais les réservoirs d'éluants sous une pression supérieure à 0,07 MPa (10 lb/po²).

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ICS-2100 Installation Instructions

Setzen Sie den Eluentbehälter auf keinen Fall einem Druck über 0,07 MPa aus.

• Use ASTM filtered, Type I (18-megohm) deionized water when preparing

eluent and regenerant.

2. Unpacking the IC System

1. Open the shipping box (see

Figure 1

layer of foam.

) and remove the top

(Remove this foam)

Figure 1. Unpacking the ICS-2100

2. Open the plastic bag that covers the IC system (see

Figure 2

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Eluent

Bottle

Figure 2. IC System Enclosed in Bag

EGC Holder (upside down)

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3. Set the eluent bottle on top of the ICS-2100 (see Figure 3

4. TURN OVER THE EGC HOLDER, and set it on top of the ICS-2100.

5. Remove the next layer of foam and remove the Ship Kit (P/N 064375) (see Figure 4

6. The bottom layer of foam has two cutouts on either side of the module; use these as handholds when lifting the IC system.

Unpacking the IC System

EGC Holder

(right-side up)

Figure 3. ICS-2100 After Installing

Eluent Bottle and EGC Holder

Ship Kit

Figure 4. IC System with Ship Kit

7. Lift the IC system off the bottom layer of foam and out of the box. Set it on a workbench.

Two or more persons should lift the IC system, which weighs 30 kg (66 lb). Lift the IC system only from each side of the cabinet bottom. Lifting from the front door will damage the door hinges.

Au moins deux personnes peuvent soulever le chromatograph ionique: il pèse 30 kg (66 lb). Ne soulevez le chromatograph ionique que par chaque côté du fond de l'armoire. Son soulèvement par la porte du panneau avant endommagera les charnières de la porte.

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ICS-2100 Installation Instructions

Das IC system wiegt über 30 kg. Daher sollte das Gerät nur von zwei oder mehr Personen angehoben werden. Greife n Sie dazu an beiden Seiten unter das Gerät. Heben Sie den Ionen chromatographen nicht an der Vordertür an, da dadurch die Türangeln beschädigt werden könnten.

8. Inspect the system for any shipping damage. Reme mber to save the shipping

container and all packing material. You will need them in future if the IC system is shipped.

3. Connecting an Autosampler (Optional)

T o connect an AS-DV or AS Autosampler to the injection valve in the IC system, follow the instructions in the appropriate section below.

Notes:

• If an optional auxiliary valve is installed in the ICS-2100, it can be used as the

injection valve, instead of the standard valve configured with each system. After connecting the autosampler to the auxiliary valve (as described in the appropriate section below), configure the valve as the injection valve in Chromeleon. For instructions, refer to Installing the ICS-1100/1600/2100 Auxiliary Valve (Document No. 065288). The document is provided with the valve.

• To connect an AS-HV Autosampler, refer to the instructions in the AS-HV

Autosampler Operator’s Manual (Document No. 065125). The manual is

provided on the Dionex Reference Library CD-ROM (P/N 053891 ).

3.1 Connecting an AS-DV Autosampler

1. Place the AS-DV to the left of the IC system on the workbench.

2. Open the IC system and the autosampler doors.

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Connecting an Autosampler (Optional)

3. Route the sample outlet line from the AS-DV through the left side slot, at the front

Sample

Outlet Line

of the IC system.

4. Connect the outlet sample line from the AS-DV to port

S(5) on the injection valve

(see Figure 5

Figure 5. IC System Injection Valve

3.2 Connecting an AS Autosampler

NOTE Dionex recommends using AS models that do not

have injection valves. If a valve is installed in the autosampler, it is switched at the same time as the valve in the IC system. This causes unnecessary wear on the autosampler valve. If your AS has an injection valve, contact Dionex for assistance.

1. Place the autosampler to the left of the IC system on the workbench.

2. Open the IC system and the autosampler doors.

AS-DV

3. Route the needle seal line that extends from the autosampler inject port through the autosampler right side slot (see Figure 6

) and then

through the IC system left side slot to the injection valve.

Route the autosampler needle seal line to the injection valve inside the IC system

Insert the waste line from the IC system injection valve here

Figure 6. AS Autosampler Drip Tray and Right Side Slot

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ICS-2100 Installation Instructions

The needle seal line from the AS autosampler inject port to the IC system injection valve is specially constructed. It consists of two tubes (one inside the other) that are seale d at the en d. This seal must be maintained.

In addition, the line is calibrated for use with the IC system. To ensure correct autosampler function, this line MUST NOT BE CUT! If replacement is required, refer to the AS operator’s manual for the required part number and replacement instructions.

4. Connect the needle seal line to port S (5) on the injection valve (see Figure 7).

Autosampler

Needle Seal

Line

Waste

Line

Figure 7. Injection Valve

A coupler (see Figure 8

) connects the green waste line from port W (6) to a 3-mm (1/8-in) ID PTFE waste line. The waste line is routed through the tubing chase to the IC system rear panel.

Green

Waste Line

Coupler

Figure 8. Injection Valve Waste Line Coupler

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Connecting an Autosampler (Optional)

5. If the coupler is not visible, grasp the green waste line where it exits the tubing chase and pull it toward the front of the system until the coupler is outside the chase.

6. Disconnect the green waste line (labeled

TO AS50 TRAY) from the coupler and

route the line to the drip tray in the autosampler compartment. You can either leave the disconnected PTFE waste line and coupler in the

tubing chase, or pull the line out from the front.

7. Bend the green waste line about 5 mm (0.2 in) from the end (to ensure it stays in the AS drip tray). When bending the tubing, be sure not to restrict the inner diameter.

8. Push the end of the green waste line into the small round opening on the right side of the AS tray (see Figure 6

Make sure the injection valve waste line is not elevated above the injection valve at any point between the valve and the autosampler drip tray.

NOTE After the AS is configured for use with the ICS-2100

(see Section 12.2 supported. In addition, all injection valve commands (whether issued from Chromeleon or the AS front panel) can control the injection valve.

), all AS injection types are

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ICS-2100 Installation Instructions

4. Unpacking the PC

1. Remove the PC and all documentation from the PC box and place them on a workbench.

2. Follow the instructions in the PC installation guide to connect the PC components.

5. Installing Chromeleon

NOTE If you purchased Chromeleon and a PC from Dionex,

omit this section and go directly to “Starting the

Chromeleon Server” on page 12.

1. To verify that the PC meets current system specifications, go to http://www.dionex.com Specifications link.

2. Verify that the Chromeleon disk contains Chromeleon version 6.80 SR6a (or later).

3. For a local PC: Log onto Windows administrator.

, select Software under Products, and click the

XP or Windows® Vi sta® as an

For a network PC: Log on as a user with local PC administrator privileges.

4. Verify that at least 250 MB of free disk space is available on the drive on which you plan to install Chromeleon.

More than 250 MB may be needed to store the large amounts of data that Chromeleon can generate. Data can also be stored at a different location (for example, on a network).

5. Insert the Chromeleon disk into the PC drive.

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Installing Chromeleon

The Chromeleon Setup screen appears (see Figure 9).

Figure 9. Chromeleon Setup screen

6. If you are installing Chromeleon on a PC that has an earlier release of

Chromeleon 6.80 already installed, click Update Chromeleon and follow the on-screen instructions.

7. If you are installing Chromeleon on a PC that does not have an earlier release of Chromeleon 6.80 installed, follow these steps to install Chromeleon:

a. On the Chromeleon Setup screen, click Exit. b. Go to the following folder on the Chromeleon disk: CM680SRx (where x

is the service release number). For example, if you are installing Chromeleon 6.80 SR6a, go to the CM680SR6a folder on the disk.

c. Double-click setup.exe and follow the on-screen instructions.

8. When asked whether to add the Server Monito r program to th e S tart up group, select Yes if the PC will be physically connected to devices or No if the PC will never be connected to devices.

9. After installing the software, restart the PC.

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ICS-2100 Installation Instructions

6. Installing the Chromeleon Software License Dongle

NOTE If your software license is provided through a

Chromeleon License Server, omit this section. Instead, follow the instructions in the Chromeleon Help to install the software license. Then, go on to “Connecting to the

Chromeleon PC” on page 13 .

1. If your software license is issued with a USB dongle, plug the dongle into a

USB port on the PC. Always connect the USB dongle to the PC, not to a

Dionex module.

Microsoft Found New Hardware Wizard (see Figure 10

Windows automatically detects the USB dongle and launches the

Figure 10. Windows Found New Hardware Wizard

2. Complete the wizard by selecting the following options:

• If asked whether Windows can connect to Windows Update to search for

software, select No, not this time.

• Accept the default option (Install the software automatically

(Recommended) option and click Next >. It is not necessary to insert a

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Installing the Software License

hardware installation disk. When the wizard has completed installing the software, click Finish.

3. Store the Chromeleon disk in a safe place, away from heat and moisture.

7. Installing the Software License

To activate the licensed features in Chromeleon, enter the license key code (an alphanumeric series) provided on the Software License key code sheet. St o re the key code sheet in a safe place.

1. Click Start on the Windows taskbar and select All Programs > Chromeleon > Chromeleon to start the Chromeleon client.

2. Select Help > About Chromeleon.

3. Click the Preferences button and select the License tab (see Figure 11

Figure 11. Chromeleon License

4. Under Copy Protection Location, select Dongle and then enter the key code, exactly as it appears on the key code sheet. Click OK.

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ICS-2100 Installation Instructions

5. The About Chromeleon Client screen should now display the serial number of the dongle, and all purchased license features (devices controlled, number of timebases, and software options) should be labeled On.

6. Click OK to close the screen.

8. Starting the Chromeleon Server

Check the Chromeleon Server icon on the Windows taskbar to determine if the server is already running:

• If the icon is crossed out in red , the server is not running. Right-click the

icon and select Start Server.

• If the icon is gray , the server is already running.

If the Server Monitor icon is not on the Windows taskbar, follow these steps to start the Chromeleon server:

1. Click Start on the Windows taskbar and select Al l Programs > Chromeleon > Server Monitor.

The Server Monitor window appears.

Figure 12. Server Not Running

2. Click Start to start the server.

3. Click Close to close the Server Monitor window. The Server Monitor icon appears on the taskbar.

NOTE Clicking the Quit Monitor button quits (or exits) the

Server Monitor program, but does not stop the server. To stop the server, click the Stop button.

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Connecting to the Chromeleon PC

9. Connecting to the Chromeleon PC

The IC system rear panel provides a USB receptacle for connecting to a USB port on the Chromeleon PC. The rear panel also provides two USB ports for connecting other Dionex USB-compliant modules to the system.

Do not turn on the power to the ICS-2100 or other USB module(s) until after you connect the USB cable.

Before connecting the USB cable, verify that Chromeleon is installed. If not, Windows will be unable to identify the new USB device when the power is turned on. To install Chromeleon, see Section 5

9.1 Connecting the IC System to the PC

1. Locate the USB cable (P/N 960779) in the IC system Ship Kit.

2. Plug the cable’s “A” connector into a USB port on the Chromeleon PC and plug the cable’s “B” connector into the USB receptacle (labeled

FROM PC) on the IC system rear panel (see Figure 13).

System

USB Cable

Figure 13. Example USB Connections: One IC System Connected to the PC

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ICS-2100 Installation Instructions

Power

Switch

FROM PC USB Receptacle

TO MODULE USB Ports

Figure 14. IC System Rear Panel

9.2 Connecting Additional USB Devices

Two USB ports on the IC system rear panel are provided for connecting additional Dionex USB-compliant modules (for example, an autosampler) to the system.

To connect an additional USB module, plug the “A” connector on a USB cable into an “A” port (labeled

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TO MODULE) on the ICS-2100 rear panel

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Connecting to the Chromeleon PC

(see Figure 14) and plug the cable’s “B” connector into the USB receptacle on the other USB module (see Figure 15

System

B A A

Dionex

USB

Module

USB Cables

Chromeleon

Dionex

USB

Module

Figure 15. Example USB Connections: Two Dionex USB Modules

Connected to the IC System

A USB 2.0 external hub can be used to connect the modules to the PC. Refer to the manual provided by the hub vendor for complete installation instructions. One or more hubs is required in the following situations:

• Use a hub if the number of USB devices in the system exceeds the

number of available USB ports.

• Use a hub if the IC system or other USB device is more than 5 meters

(16 ft) from the PC.

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The USB standard limits the USB cable length to 5 meters (16 ft). Each USB device can be separated from the PC by no more than five hubs. Thus, each USB device can be located no more than 30 meters (32 yds) from the PC.

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ICS-2100 Installation Instructions

10. Connecting the Power Cord

1. Verify that the main power switch on the rear panel of the ICS-2100 (see

Figure 14

accidentally when the module is unpacked.)

2. Connect the power cord (IEC 320 C13) (ordered separately) from the main power receptacle on the rear panel to a grounded power source. The IC system power supply is auto-sensing, so no adjustment is required to select the line voltage.

) is turned off. (The main power switch may be turned on

SHOCK HAZARD—To avoid electrical shock, use a grounded receptacle. Do not operate the IC system or connect it to AC power mains without an earthed ground connection.

The power supply cord is used as the main disconnec t device. Make sure the socket-outlet is located near the IC system and is easily accessible.

Operation at AC input levels outside of the specified operating voltage range may damage the IC system.

DANGER D'ÉLECTROCUTION—Pour éviter toute électrocution, il faut utiliser une prise de courant avec prise de terre. Ne l'utilisez pas et ne le branchez pas au secteur C.A. sans utiliser de branchement m is à la terre.

Le cordon d'alimentation principal est utilisé comme dispositif principal de débranchement. Veillez à ce que la prise de base soit située/installée près du module et facilement accessible.

STROMSCHLAGGEFAHR—Zur Vermeidung von elektrischen Schlägen ist eine geerdete Steckdose zu verwenden. Das Gerät d arf nicht ohne Erdung betrieben bzw. an Wechselstrom angeschlossen werden.

Das Netzkabel ist das wichtigste Mittel zur Stromunterbrechung. Stellen Sie sicher, daß sich die Steckdose nahe am Gerät befindet und leicht zugänglich ist.

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Installing the ICS-2100 Device Driver

11. Installing the ICS-2100 Device Driver

Before turning on the system power, verify that Chromeleon software is installed. If Chromeleon is not installed first, Windows will be unable to identify the new USB device. For Chromeleon installation instructions, see Section 5

1. If you have not already done so, turn on the PC power.

2. For a local PC: Log onto Windows XP or Windows Vista as an

administrator. For a network PC: Log on as a user with local PC administrator privileges.

3. If the Chromeleon Server is not running, start the server (see Section 8

4. Press the power switch on the ICS-2100 rear panel (see Figure 14

) to turn on

the power.

5. Turn on the power to the autosampler (if installed).

6. Windows automatically detects the new USB devices. A message flashes on the screen to inform you that new hardware was found.

The Found New Hardware Wizard appears (see Figure 16

). This wizard

installs the new hardware into the Windows Device Manager.

Figure 16. Windows Found New Hardware Wizard: IC System Device

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ICS-2100 Installation Instructions

7. Complete the wizard for each new device by selecting the following options:

• If asked whether Windows can connect to Windows Update to search for

software, select No, not this time.

• Accept the default option (Install the software automatically) and click

Next >. It is not necessary to insert a hardware installation disk.

8. If any additional devices were found (for example, an autosampler) complete the wizard for the device.

12. Setting Up Chromeleon

NOTE This section provides brief instructions for setting up

Chromeleon. For details about any of these steps, refer to the Chromeleon Help.

12.1 Creating a Timebase

1. Click Start on the Windows taskbar and click All Programs > Chromeleon > Server Configuration to start the Chromeleon Server

Configuration program.

2. Select Edit > Add Timebase. The New Timebase dialog box appears.

3. Type a name for the timebase (for example, ICS-2100) (see

Figure 17

Figure 17. Example New Timebase Dialog Box

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Setting Up Chromeleon

4. (Optional) Select the Create Folder Structure for Your Data

check box. This creates a standardized folder structure in the

Chromeleon Browser for the data related to your timebase.

5. Click OK.

The new timebase appears under the server name.

Figure 18. Example Server Configuration with New Timebase

6. Right-click the new timebase and select Add Device.

The Add device to timebase dialog box appears.

7. Under Manufacturers, select Dionex IC: Integrated Systems.

8. Under Devices, select ICS-2100 IC System and click OK. The Properties dialog box for the IC system appears. The General tab

page shows the Device Name (Pump_ECD) and the Mode (Live).

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ICS-2100 Installation Instructions

9. Click the arrow next to Module Serial No. In the list, select the serial

number of the system you are configuring (see Figure 19

Figure 19. Example Properties Dialog Box: General Tab Page

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12.2 Configuring the Device Properties

NOTE For details about the settings on any of the

Properties tab pages, click the Help button.

1. Click the Options tab and verify that the options installed in your IC system are enabled.

Setting Up Chromeleon

Figure 20. Example Properties Dialog Box: Options Tab Page

To configure an auxiliary power su pply (optional):

To operate an electrolytic device such as a water polisher, you can configure the EGC-2 power supply as an auxiliary power supply . The power supply operates in constant current mode and can be configured from 0 to 200 mA, with a maximum voltage of 35 V.

• On the Options tab page, select the AuxPower Supply check box

(see Figure 20

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) and enter the Maximum Current allowed for the

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ICS-2100 Installation Instructions

electrolytic device (refer to the documentation provided with the device).

• Connect the device to the EGC-2 connector on top of the ICS-

2100.

After the auxiliary power supply is configured, information about the power supply (current, on, off, etc.) is displayed in Chromeleon and on the front panel touch screens.

2. Click the Inject Valve tab and verify that the correct device is

assigned to control the injection valve in the ICS-2100.

• If the timebase will include an AS or AS-DV, the Controlled By

setting for Pump_InjectValve must be AS (see Figure 21

Figure 21. Inject Valve Properties: Inject Valve Controlled by the AS

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Setting Up Chromeleon

• If the timebase will include another model of autosampler (or no

autosampler), the Controlled By setting must be ICS-2100 (see

Figure 22

Figure 22. Inject Valve Properties: Inject Valve Controlled

To change a Controlled By setting, select the Pump_InjectValve name, press F2, and select the device.

by the IC System

3. After selecting the device properties, click OK to close the dialog box. The IC system is added to the timebase (see Figure 23

Figure 23. Example Server Configuration

with IC System Added to the Timebase

4. If you are configuring an autosampler, add the autosampler to the timebase, as described for the IC system.

5. When the timebase is complete, select File > Save Installation.

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ICS-2100 Installation Instructions

6. You can ignore the “no inject device installed” warning, if it appears. This simply means that the timebase does not include an autosampler. This message appears if you are using manual injections. Close the Server Configuration Check dialog box.

7. Exit the Server Configuration program.

12.3 Connecting to the Panel Tabset

1. Click Start on the Windows taskbar and select All Programs > Chromeleon > Chromeleon to start the Chromeleon client.

The Chromeleon main window and Browser appear (see Figure 24

Figure 24. Chromeleon Main Window and Browser

2. Select View > Default Panel Tabset to display the panel tabset.

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Setting Up Chromeleon

3. Click the ICS-2100 tab on the panel tabset to view the IC system

panel (see Figure 25

Figure 25. IC System Panel on the Chromeleon Panel Tabset

NOTE If an autosampler is installed, a separate Control

panel for the autosampler is included in the panel tabset.

12.4 Verifying Chromeleon Communication

Verify that Chromeleon is communicating with the IC system by clicking the

Inject and then the Load button on the panel. If communication is

occurring, you will hear the valve actuate as it changes position.

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ICS-2100 Installation Instructions

13. Installing and Plumbing the Columns and Suppressor

This section provides brief installation and plumbing instructions for the columns and suppressor. For detailed start-up and installation instructions, refer to the column and suppressor manuals. The manuals are provided on the Dionex Reference Library CD-ROM (P/N 053891).

For reference, Figure 26 completed.

ELUENT

shows the system flow schematic after all plumbing is

OUT

WASTE

SAMPLE

VALVE

DAMPER

COND

CELL

HEAT EXCHANGER

COLUMN HEATER

SEPARATOR COLUMN

SUPPRESSOR

GUARD

WASTE

OUT

WASTE

DUCER

PUMP HEAD

OUT

PUMP HEAD

Figure 26. IC System Plumbing Schematic

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Installing and Plumbing the Columns and Suppressor

13.1 Unpacking the Columns and Suppressor

1. Remove the guard column, separator column, and suppressor from their boxes.

2. Remove the fitting plugs from the ends of each column and from all ports on the suppressor.

3. Discard any tubing temporarily connecting the suppressor ports.

13.2 Setting Up the Column Heater

Note for 2-mm columns:

For best results with 2-mm columns, install a microbore heat exchanger (P/N 060943) in the column heater. In addition, use red 0.125-mm (0.005-in) ID PEEK™ tubing (P/N 044221), rather than the standard black PEEK tubing, for tubing connections between the following components:

• Injection valve and column heater heat exchanger

• Guard column and separator column

• Separator column and detector cell

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ICS-2100 Installation Instructions

To set up the column heater

1. Cut off the two shipping ties from the column heater and remove them (see Figure 27

Thumbscrew (2)

Shipping Tie (2)

Figure 27. Column Heater with Shipping Ties

2. Loosen the two thumbscrews on the heater cover (they remain attached to the cover).

3. Pull the heater cover straight out to remove it.

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Installing and Plumbing the Columns and Suppressor

4. Unscrew the six thumbscrews on the top metal plate (see

Figure 28

the plate.

) and remove

Thumbscrew (6)

Figure 28. Column Heater with Cover Removed

13.3 Plumbing the Columns

1. Remove the coupler connecting the lines labeled GUARD IN and

GUARD OUT.

2. Connect the arrow on the guard column label; it should point down (flow away from the injection valve).

3. Connect the

4. Push the guard column onto the back column clip.

5. Remove the coupler connecting the

6. Connect the separator column outlet. Check the arrow on the column label. It should point up (flow toward the cell).

7. Push the separator column onto the front column clips.

8. Replace the top metal plate on the column heater.

GUARD IN line to the guard column inlet. Check the

GUARD OUT line to the guard column outlet.

COL IN to the separator column inlet. Connect COL OUT to

COL IN and COL OUT lines.

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ICS-2100 Installation Instructions

13.4 Installing the Suppressor

1. Before installing the suppressor, hydrate it as instructed in the suppressor manual. Suppressor manuals are provided on the Dionex Reference Library CD-ROM (P/N 053891).

2. Locate the suppressor mounting tabs on the component panel (see

Figure 29

the opening between the tabs and pull the yellow connector and attached cable slightly out of the opening.

Connect this cable to the cable from the suppressor (align the pins on the two connectors and push them together).

). Reach into

Suppressor

Mounting Tabs Suppressor

Cable Connector

Guideline for AES

Guideline for SRS

Figure 29. Suppressor Mounting Tabs

and Guidelines

3. Orient the suppressor with the REGEN OUT fitting on top and the cable to the right.

4. Feed the cable into the opening in the component panel.

5. Align the top of the suppressor with the guideline printed on the component panel (see Figure 29

) and align the slots on the rear of the

suppressor with the tabs on the panel.

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Connecting the Waste Lines

6. Press the suppressor onto the tabs and then slide it down to lock it in place. Pull slightly on the center of the suppressor to verify that it is securely fastened.

Figure 30

installed on the component panel.

shows an SRS® suppressor

7. Remove the coupler connecting the

ELUENT IN

and ELUENT OUT lines and connect these lines to the corresponding ports on the suppressor.

8. Remove the fitting plug from the REGEN IN line and connect it to the

REGEN IN port on the suppressor.

9. Do not connect the

REGEN OUT port until after completing the

eluent generator setup (Section 18

10. Replace the cover on the column heater, making sure the line is aligned with the slot on the side of the heater cover.

14. Connecting the Waste Lines

Figure 30. Suppressor Installed

(SRS Shown)

GUARD IN

1. Untape the coiled waste lines from the rear panel. Place the ends of the PUMP

WASTE

2. Direct the

and TO WASTE OUT lines into a waste container.

WASTE, GAS SEPARATOR line from the top of the IC system to the

rear panel. Snap the line onto one of the tubing clips on the rear panel. Connect the line to the waste, gas separator tube (see Section 14.1

NOTE To prevent waste from siphoning back into the system,

check the lines periodically to be sure they are not bent, pinched, or elevated at any point.

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ICS-2100 Installation Instructions

14.1 Installing the Waste, Gas Separator Tube

1. Locate the waste, gas separator tube assembly in the IC

(from the suppressor)

Waste, Gas

Separator Line

system Ship Kit.

Waste,

2. Connect the

GAS SEPARATOR

WASTE,

from the suppressor to one of the 3-mm (1/8in) ID white PTFE

line

Open to

the air

Gas Separator Tube Assembly

Connect this line to a second system, if needed.

lines on the waste, gas separator tube assembly (see

Figure 31

Figure 31. Waste, Gas Separator Tube Installation

Waste

3. Place the waste, gas separator tube assesmbly into the waste container. Make sure the tubing junction (where the white PTFE tubing meets the black polyethylene tubing) is above the top of the container and that the waste, gas separator tube and the waste container are open to the atmosphere.

DO NOT CAP THE WASTE CONTAINER: In the ICS-2100, the Atlas Electrolytic Suppressor and Self-Regenera ting Suppressor (SRS) use an electrolytic process that results in the production of small amounts of oxygen or hydrogen gas. To ensure that the gas is not trapped in a closed container and allowed to concentrate, install a 1.3-cm (0.52-in) ID black waste, gas separator tube (P/N 045460) in an uncapped waste container. Connect the Waste, Gas Separator line to the waste tube (see Figure 31

NE FERMEZ PAS LE CONTENEUR DE GAZ RÉSIDUEL: Le ICS-2100, le Atlas Electrolytic Suppressor, et le Self-Regenerating Suppressor (SRS) travaillent avec un proces d´électrolyse, qu i produit des petite s quantités de gaz d´oxygène ou d´hydrogène. Afin de garantir que le gaz ne soit pas enfermé dans un conteneur fermé et puisse s´y concentrer, connectez un tube noir à gaz résiduel (diamètre intérieur = 1,3 cm; n° de commande 045460) à un conteneu r ouvert (non fermé). Connectez le conteneur résiduel au tube résiduel/gaz séparateur (désigné: « Waste, Gas Separator»).

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Setting Up the Eluent Reservoir

VERSCHLIESSEN SIE DEN ABFALLBEHÄLTER NICHT: Der ICS-2100 , Atlas Electrolytic Suppressor und Self-Regenerating Suppressor (SRS) verwenden einen Elektrolyseprozess, wodurch kleine Mengen an Sauerstoff und Wasserstoff entstehen. Führen Sie einen schwarzen Gasabscheiderschlauch (ID = 1,3 cm; Bestell-Nr. 045460) in einen offenen (unverschlossenen) Abfallbehälter, damit sich das Gas nicht in einem geschlossenen Behälter sa mmelt und aufkonzentriert. Verbinden Sie die mit Waste, Gas Separator bezeichnete Leitung mit dem Abfallschlauch.

15. Setting Up the Eluent Reservoir

1. Rinse the eluent reservoir with ASTM filtered, Type I (18-megohm) deionized water.

2. Fill the reservoir with ASTM filtered, Type I (18-megohm) deionized water.

3. Place the reservoir on top of the IC system.

4. Install the cap and handtighten.

5. Connect the liquid lines from the IC system to the corresponding lines exiting the eluent bottle.

NOTE The IC system does not require pressurized reservoirs.

However, if eluent is manually degassed or is sensitive to contamination, Dionex recommends pressurizing the reservoir with helium or nitrogen. See Section 22 instructions.

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for

Page 38

ICS-2100 Installation Instructions

16. Entering the Eluent Level

After filling the reservoir, enter the volume of liquid in the reservoir.

1. On the IC system

panel on the Chromeleon panel tabset, click the Pump

Settings button. The Pump Settings window appears (see Figure 32

Enter the

volume of

liquid in the

reservoir

(in liters)

Figure 32. Pump Settings Window: Setting the Eluent Level

Note: The Eluent Bottle field and slider gauge are updated as the liquid is used up.

2. Use the slider to enter the volume (in liters), or enter the volume directly into

the Eluent Bottle box.

The IC system determines the eluent usage by monitoring the flow rate and the length of time the pump is on, and updates the

Eluent Bottle volume as the eluent

is used up. A warning appears if the level falls below 200 mL. Warnings are repeated at 100 mL and 0 mL.

In order for the level displayed in the Eluent Bottle box and gauge to be accurate, you must enter the level when the reservoir is filled. The IC system does not automatically detect when the reservoir is filled, nor when it is empty.

If preferred

, you can enter the eluent bottle level on the touch screen HOME page,

instead of on the Chromeleon Control panel.

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17. Priming the Pump

1. Verify that the eluent reservoir is filled, the reservoir cap is installed and handtightened, and the liquid line from the IC system to the reservoir cap is connected (see Section 15

2. Verify that the waste lines are directed to a waste container.

3. The priming procedure consists of two parts:

• Priming the eluent lines with a syringe (see Section 17.1). Perform this

procedure at initial installation, after changing eluents, or when eluent lines are empty.

• Priming the pump heads with the Prime button (see Section 17.2).

Perform this procedure after the eluent lines are primed.

17.1 Priming the Eluent Lines with a Syringe

NOTE Prime the eluent lines after initial installation, after

changing eluents, or when eluent lines are empty.

Priming the Pump

1. Verify that the pump is turned off.

2. Connect a 10 mL syringe (P/N 054578) to the priming valve port on the primary pump head (see Figure 33

Secondary

Pump Head

Waste Valve

(close)

Port

10 mL Syringe

(P/N 054578)

Figure 33. Pump Head Valve Settings for Priming the Eluent Lines

Priming Valve

(open)

Primary Pump Head

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ICS-2100 Installation Instructions

3. Open the priming valve by turning it one-quarter to one-half turn counterclockwise.

4. On the IC system

panel on the Chromeleon panel tabset, click the

Pump Settings button. The Pump Settings window appears (see Figure 34

Figure 34. Pump Settings Window

5. Under

Eluent Flow Valve, click Open.

6. Draw the syringe back to begin pulling eluent through the flow path. It may take several syringe draws to remove all air or previous eluent from the tubing. Draw out a minimum of 20 mL of eluent to ensure that the vacuum degas assembly (if installed) is primed.

7. After priming the lines thoroughly, close the priming valve. Do not overtighten the priming valve.

8. Under

36 Doc. 065294-01 3/09

Eluent Flow Valve, click Closed.

Page 41

Priming the Pump

(

)

17.2 Priming the Pump Heads with the Prime Button

NOTE Perform this procedure after priming the eluent

lines (see Section 1 7.1

1. Close the priming valve on the primary pump head (see Figure 35).

Secondary

Pump Head

Waste Valve

(open)

Priming Valve

Primary Pump Hea

Figure 35. Pump Head Valve Settings for Priming the Pump Heads

2. Open the waste valve on the secondary pump head by turning the knob one-quarter to one-half turn counterclockwise (see Figure 35 Opening the valve directs the eluent flow path to waste and eliminates backpressure.

3. On the Pump Settings window, click the the waste valve is open by clicking

Prime button. Confirm that

OK when the reminder message

appears. The pump will begin pumping at approximately 3 mL/min.

4. Continue priming the IC system until no air bubbles are exiting the pump waste line.

5. On the Pump Settings window, click

Pump Off.

6. Close the waste valve. Do not overtighten. The pump is now ready for operation.

NOTE If the two standard priming procedures described

above are unsuccessful, prime the pump with isopropyl alcohol. See Chapter 5 in the IC system operator’s manual for instructions.

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ICS-2100 Installation Instructions

18. Setting Up the Eluent Generator

Before Beginning

Remove the EluGen® cartridge (EGC) and the other items to be installed from their shipping containers. Depending on the type of eluent to be generated, the other items may include a Continuously Regenerated Trap Column (CR-TC), an EPM Electrolytic pH Modifier, and/or an EGC CO

NOTE The EGC degas assembly is installed inside the EGC

holder at the factory.

The EluGen cartridge contains one of the following: a corrosive bas e (KOH, LiOH, or NaOH), a corrosive acid (MSA), or a concentrated

solution. Wear protective eyewear and gloves when handling

2CO3

the cartridge. La cartouche d'EluGen contient un de ce qui suit: une base corrosive

(KOH, LiOH, ou NaOH), un acide corrosif (MSA), ou une solution concentrée de K

manipulant la cartouche. Die EluGen-Kartusche enthält eine korrosive Base (KOH, Li OH oder

NaOH), oder eine korrosive Säure (MSA). Tragen Sie daher beim Umgang mit der Kartusche eine Schutzbrille und Handschuhe.

. Porter des lunettes et des gants protectives en

2CO3

Mixer.

Refer to the EluGen cartridge, CR-TC, and EPM manuals for the optimal operating conditions for your application. The manuals are provided on the Dionex Reference Library CD-ROM (P/N 053891).

18.1 Eluent Generator Setup for KOH, LiOH, MSA, and NaOH Eluents

Follow the instructions in this section if you are installing a KOH, LiOH, MSA, or NaOH EluGen cartridge and a CR-TC.

• If you are installing a K

Mixer (for generating carbonate/bicarbonate eluent), go to

Section 18.2

• If you are installing a K

generating carbonate eluent), go to Section 18.3

38 Doc. 065294-01 3/09

cartridge, an EPM, and an EGC CO

2CO3

cartridge and an EGC CO3 Mixer (for

2CO3

Page 43

Setting Up the Eluent Generator

Overview

The eluent generator setup procedure for KOH, LiOH, MSA, or NaOH eluents consists of the following main steps:

• Installing the CR-TC.

• Installing the EluGen cartridge.

• Connecting the EluGen cartridge vent line.

• Recording the EluGen cartridge serial number in Chromeleon.

• Conditioning the EluGen cartridge.

• Hydrating the CR-TC.

• Completing the eluent generator setup.

Items Required to Complete the Setup Procedure

In addition to the EluGen cartridge and the CR-TC, you will need the following items to complete the eluent generator setup.

Item P/N Use To

Yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (supplied in the ICS-2100 Ship Kit (P/N 064375))

EluGen Cartridge Vent Line Tubing Kit (supplied with the cartridge)

10-32 to 1/4-28 coupler (supplied in the ICS-2100 Ship Kit)

Temporary waste container (beaker, bottle, etc.) (not supplied)

10-32 fittings 10-32 ferrules

1/8-in fittings 1/8-in ferrules

053765 Provide backpressure during

setup procedures.

063092 Vent the cartridge during

operation.

042806 Provide a temporary connection

during setup procedures.

n/a Collect waste during setup

procedures.

043275 043276

052267 048949

Complete the connections.

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ICS-2100 Installation Instructions

Figure 36 illustrates the ICS-2100 after the EluGen cartridge is installed.

IN EGC OUT

EluGen

Cartridge

(EGC)

EGC Holder

EGC and CR-TC Cable Connections

Figure 36. ICS-2100 with an EluGen Cartridge Installed (Top View)

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Setting Up the Eluent Generator

(2)

Figure 37 illustrates the liquid flow path through the eluent generator

components after all setup steps are complete and the eluent generator is ready for operation.

EGC VENT

EGC II

KOH, LiOH,

MSA, or NaOH

Send to waste

Inlet Outlet

EGC

OUT

TO CR-TC

ELUENT IN

(red)

TO PUMP/

DAMPER

Pre-plumbed

Pump Pulse

Damper

DEGAS

ELUENT OUT

TO DEGAS ELUENT IN

TO DEGAS

ELUENT OUT

EGC

DEGAS

CR-TC

Eluent In

Regen In

Regen Out

(

Eluent

Out

TO CR-TC/EPM

ELUENT OUT

(yellow)

)

TO CR-TC

REGEN OUT

(blue)

Back-

pressure

Coil*

TO INJ

ALVE IN - P/

ELUENT OUT

Connect to

Inject Valve

Port P

*Add backpressure coils, if needed, to create a system backpressure of between 14 and 16 MPa (2000 and 2300 psi).

TO SRS/AES REGEN OUT

Connect to

Suppressor

en Out

Figure 37. Eluent Generator Flow Schematic with CR-TC:

Use for Generating KOH, LiOH, MSA, or NaOH Eluent

WASTE,

GAS

SEPARATOR

Connect to

Gas Separato

Assembl

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ICS-2100 Installation Instructions

Installing the CR-TC

1. Tilt the cartridge holder to view the bottom of the holder and brace the holder against the back corner of the ICS-2100 (see

Figure 38

Figure 38. Cartridge Holder (Tilted to View the Bottom)

Metal ball stud for mounting the CR-TC

2. Before installing the CR-TC, note the metal ball stud inside the bottom of the cartridge holder (see Figure 38

E= Electrical connection (2) (Do not remove)

P=Port (4) (Remove plugs)

)

and the bracket on the CR-TC mounting plate (see Figure 39 These are used to mount the CR-TC

inside the holder after it is plumbed.

Figure 39. CR-TC

Bracket for mounting the CR-TC on the holder

3. Remove the plugs from the four ports, labeled “P” in Figure 39. Do not remove the two electrical connections, labeled “E” in the figure.

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Setting Up the Eluent Generator

4. With the CR-TC oriented as shown in Figure 40, connect each line to the corresponding port on the CR-TC.

NOTE To help retain the fittings and ferrules on the lines,

remove the coupler from each line just before connecting the line to a port.

Regen Out

Eluent

Eluent In

Regen In

Out

Figure 40. Plumbed CR-TC

5. Check that none of the lines are caught under the CR-TC and then push the CR-TC onto the metal stud inside the EGC holder (see

Figure 41

). Push down until the CR-TC snaps into place.

Figure 41. CR-TC Installed in Holder

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ICS-2100 Installation Instructions

6. Feed the CR-TC cable through the slot on the side of the EGC holder (see Figure 42

Figure 42. Installing the CR-TC

CR-TC Cable

7. Turn the EGC holder right-side up and set it back into position.

Installing the EluGen Cartridge

1. Orient the EluGen cartridge with the fittings facing up and remove the plugs from the EluGen cartridge

OUTLET ports (see

Figure 43

44 Doc. 065294-01 3/09

INLET and

OUTLET

INLET

Figure 43. EluGen Cartridge Inlet and Outlet Ports

Page 49

Setting Up the Eluent Generator

2. Set the EluGen cartridge in the service area on top of the ICS-2100 and orient it with the cable toward the EGC holder (see Figure 44

EGC Cable

Figure 44. EluGen Cartridge in Service Area

3. Pull the coiled black

EGC IN and EGC OUT lines out slightly from the

cartridge holder .

4. Disconnect the

IN line from the

union that connects it to the

EGC OUT

EGC

EGC IN

INLET Port

line.

5. Connect the EGC (see Figure 45

Doc. 065294-01 3/09 45

EGC IN to

INLET port

Figure 45. EGC Inlet Port Connections

Page 50

ICS-2100 Installation Instructions

6. Locate the yellow

0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) in the ICS-2100 Ship Kit (P/N 064375).

7. Connect one end of this coil to the EGC

OUTLET

Figure 46

port (see

). Leave the other end of the coil unconnected.

Backpressure Coil

OUTLET Port

Figure 46. EGC Outlet Connection for

Conditioning the EGC

NOTE The backpressure coil connection is temporary; it is

required only during the EluGen cartridge conditioning procedure described in “Conditioning

the EluGen Cartridge” on page 50.

8. Turn the EluGen cartridge over (with the fittings facing down). Shake the EluGen cartridge vigorously , and tap it with the palm of your hand 10 to 15 times. Check that all bubbles trapped in the eluent generation chamber are dislodged.

The EluGen cartridge contains one of the following: a corrosive bas e (KOH, LiOH, or NaOH), a corrosive acid (MSA), or a concentrated

solution. Wear protective eyewear and gloves when ha ndling

2CO3

the cartridge. La cartouche d'EluGen contient un de ce qui suit: une base corrosive

(KOH, LiOH, ou NaOH), un acide corrosif (MSA), ou une solution concentrée de K

manipulant la cartouche. Die EluGen-Kassette enthält eine korrodierende Base (KOH, LiOH

oder NaOH), eine korrodierende Säure (MSA) oder eine konzentrierte

-Lösung. Tragen Sie daher beim Umgang mit Kassette eine

2CO3

Schutzbrille und Handschuhe.

. Porter des lunettes et des gants protectives en

2CO3

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Page 51

Setting Up the Eluent Generator

9. Slide the EluGen cartridge down into the holder until it stops. Make sure the cable and backpressure coil remain out of the holder (see Figure 47

NOTE To avoid crimping the

lines inside the holder, do not twist the cartridge when you slide it into the holder. The lines are designed to coil inside the holder.

Figure 47. Installing the EluGen Cartridge in the Holder

10. Remove the plug from the

EGC-1 connector on

top of the ICS-2100.

11. Orient the cartridge’s blue 4-pin electrical cable connector as shown in Figure 48 Push it onto the

EGC-1

connector on the ICS-

2100. Twist the ring on the cable connector fingertight to secure it.

Figure 48. Connecting the EluGen Cartridge Cable

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ICS-2100 Installation Instructions

Connecting the Vent Line

1. Locate the vent line tubing kit (P/N 063092) supplied with the EluGen cartridge. Slide the label onto one end of the tubing and then push the barbed luer lock onto the tubing.

2. Unscrew and remove the plug from the vent opening on the side of the EluGen cartridge (see Figure 49

Vent Plug

(remove)

Figure 49. EluGen Cartridge Vent Plug

3. Screw the luer adapter onto the vent opening.

Luer Adapter

Luer Lock

Remove the cap on the luer adapter by twisting. Save the cap.

EGC Vent Line

4. Twist the luer lock and EluGen cartridge vent line into the luer adapter (see

Figure 50

NOTE To prevent leaks from the EluGen cartridge, always

Figure 50. Installing the EluGen Cartridge Vent Line

replace the luer adapter cap before moving the EluGen cartridge.

5. Direct the EluGen cartridge vent line to the rear of the ICS-2100 and snap it onto one of the tubing clips on the rear panel.

6. Direct the vent line to the waste container and insert it down into the waste, gas separator tube about 10 to 15 cm (4 to 6 in).

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Page 53

Setting Up the Eluent Generator

Recording the EluGen Cartridge Serial Number in Chromeleon

The serial number is printed on the EluGen cartridge label.

1. Open the Chromeleon Server Configuration program (select Start > Programs > Chromeleon > Server Configuration).

2. Double-click the ICS-2100 system icon. The properties dialog box for the ICS-2100 appears.

3. Click the Eluent Generator tab (see Figure 51

4. Select the EGC-1 check box and enter the EluGen cartridge serial number.

5. Click OK.

Figure 51. Eluent Generator Configuration Properties: OH Example

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ICS-2100 Installation Instructions

Conditioning the EluGen Cartridge

After completing the installation steps described in the previous sections, condition the EluGen cartridge for 30 minutes.

1. Set a temporary waste container, such as a beaker, on top of the ICS-2100 and direct the yellow backpressure coil from the EluGen cartridge

OUTLET port to the container.

2. On the ICS-2100 touch screen

HOME page, set the pump flow rate to

1.00 mL/min.

3. Touch the

GENERATOR

EGC button on the HOME page to open the ELUENT

page. Set the EGC concentration to 50 mM.

4. Verify that the suppressor and CR-TC are off.

To avoid damaging the suppressor and CR-TC, always turn off the suppressor and CR-TC before condition ing the EluGen c artridge. Th e pump flow is on during conditioning, ho wever, no flow reaches the suppressor or CR-TC.

5. Verify that the selected on the ICS-2100 touch screen

ELUENT GENERATOR page. Disabling these options lets you turn on

Automatically turn on with pump options are not

SUPPRESSOR page and

the pump, without automatically turning on the suppressor and CRTC.

6. Verify that the eluent reservoir is filled with ASTM filtered, Type 1 (18 megohm-cm) deionized water (see Section 15

7. Turn on the pump.

8. Turn on the EluGen cartridge.

9. Run at the selected settings (1.00 mL/min at 50 mM) for 30 minutes.

10. Turn off the EluGen cartridge.

11. Turn off the pump.

12. Disconnect the EluGen cartridge cable.

13. Remove the backpressure tubing from the waste container and remove the waste container.

14. Lift the EluGen cartridge out of the holder, turn it over, and set it in the service area.

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Setting Up the Eluent Generator

15. Disconnect the backpressure coil from the EluGen cartridge port

16. Connect the EGC

OUT OUTLET port (see

Figure 52

17. Turn over the EluGen cartridge. Check for bubbles; if necessary, shake and tap the EluGen cartridge to remove bubbles.

OUTLET

line to the

Figure 52. EGC Inlet and Outlet Connections

18. Reinstall the EluGen cartridge in the holder.

19. Do not reconnect the EluGen cartridge cable until after hydrating the CR-TC.

Hydrating the CR-TC

Always hydrate the CR-TC before initial operation. This requires running ASTM filtered, T ype 1 (18 megohm-cm) deionized water through the CRTC for 10 minutes, while bypassing the columns and suppressor.

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ICS-2100 Installation Instructions

Figure 53 illustrates the liquid flow path through the CR-TC during the

hydration procedure.

Deionized water

in from pump

TO EGC

OUT

TO CR-TC

ELUENT IN

(red)

DEGAS

ELUENT OUT

TO DEGAS

ELUENT IN

TO DEGAS

ELUENT OUT

EGC

DEGAS

CR-TC

Eluent In

Regen In

Regen Out

(

Eluent

Out

TO CR-TC/EPM

ELUENT OUT

(yellow)

TO CR-TC

REGEN OUT

)

(blue)

10-32 to

TO INJ VALVE IN - P/ ELUENT OUT

1/4-28 Coupler

TO SRS/AES REGEN OUT

Figure 53. CR-TC Plumbing for Hydration

1. Disconnect the

TO INJ VALVE IN - P/ELUENT OUT line from port P (2)

on the injection valve.

2. Disconnect the

TO SRS/AES REGEN OUT line from the suppressor.

3. Use a 10-32 to 1/4-28 coupler (P/N 042806) to connect the

VALVE IN - P/ELUENT OUT

line to the SRS/AES REGEN OUT line.

4. Verify that the suppressor is off.

WASTE,

GAS

SEPARATOR

TO INJ

To avoid damaging the suppressor, always turn off the suppressor before hydrating the CR-TC. The pump flow is on during conditio ni ng , however, no flow reaches the suppressor.

5. Verify that the selected on the ICS-2100 touch screen

Automatically turn on with pump option is not

SUPPRESSOR page. Disabling

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Setting Up the Eluent Generator

this option lets you turn on the pump, without automatically turning on the suppressor.

6. Turn on the pump and set the flow to the rate recommended for your application.

7. Pump deionized water through the CR-TC for at least 10 minutes.

8. Turn off the pump and remove the coupler.

Completing the Eluent Generator Setup

1. Reconnect the TO SRS/AES REGEN OUT line to the REGEN OUT port on the suppressor.

2. Reconnect the

TO INJ VALVE IN - P/ELUENT OUT line to port P (2) on

the injection valve.

3. Reconnect the EluGen cartridge cable.

4. Remove the plug from the

CR-TC connector on top of the ICS-2100

5. Orient the CR-TC’s black 3-pin electrical cable connector as shown in Figure 48 Push it onto the

connector on the

CR-

ICS-2100. Twist the ring on the cable connector and fingertighten to secure it.

Figure 54. Connecting the CR-TC Cable

NOTE Backpressure tubing may be required between the

injection valve and the EGC degas eluent outlet. The EluGen cartridge requires 14 to 16 MPa (2000 to 2300 psi) of system backpressure for removal of electrolysis gas from the eluent produced by the cartridge. See Section 19.1

for details.

6. Go on to Section 19, “Equilibrating the System.”

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ICS-2100 Installation Instructions

18.2 Eluent Generator Setup for Carbonate/Bicarbonate Eluent

Follow the instructions in this section if you are installing a K2CO3 cartridge, an EPM, and an EGC CO carbonate/bicarbonate eluent).

Mixer (for generating

• If you are installing a K

generating carbonate eluent), go to Section 18.3

cartridge and an EGC CO3 Mixer (for

2CO3

• If you are installing a KOH, LiOH, MSA, or NaOH EluGen cartridge

and a CR-TC, go to Section 18.1

Overview

The eluent generator setup procedure for carbonate/bicarbonate eluent consists of the following main steps:

• Installing the EluGen cartridge.

• Connecting the EluGen cartridge vent line.

• Installing the EPM.

• Plumbing the EPM for conditioning.

• Recording the EluGen cartridge and the EPM serial numbers in

Chromeleon.

• Conditioning the EluGen cartridge and the EPM.

• Installing the EGC CO

• Temporarily filling the EGC CO

• Filling the EGC CO

Mixer.

Mixer with deionized water.

Mixer with eluent of the required concentration.

• Completing the eluent generator setup.

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Page 59

Setting Up the Eluent Generator

Items Required to Complete the Setup Procedure

The following items are required to setup the EluGen cartridge, EPM, and EGC CO Ship Kit (P/N 064375).

Item P/N Used To

EPM mounting bracket 063624 Mount the EPM on the EGC holder.

Mixer. Except as noted, all items are supplied in the ICS-2100

35 cm (14 in) of black 0.25-mm (0.010-in) ID PEEK tubing

30 cm (12 in) of black 0.25-mm (0.010-in) ID PEEK tubing

28 cm (11 in) of clear 1.58-mm (0.062-in) ID PTFE tubing

EluGen Cartridge Vent Line Tubing Kit (supplied with the cartridge)

Yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil

EGC CO

Mixer mounting clips 063642 Install the mixer.

Phillips head screwdriver (not supplied)

35 cm (14 in) of black 0.25-mm (0.010-in) ID PEEK tubing

60 cm (24 in) of black 0.25-mm (0.010-in) ID PEEK tubing

064340 Lengthen the line between the EGC degas

eluent inlet and the EPM

ELUENT OUT port.

064334 Lengthen the line between the EluGen

cartridge outlet and the EPM

ELUENT IN

port.

064337 Lengthen the line between the EGC degas

regenerant inlet and the EPM

REGEN OUT

port.

063092 Vent the cartridge during operation.

053765 Provide backpressure during setup

procedures.

n/a Install the EGC CO

Mixer mounting clips.

064346 Connect the EGC degas eluent outlet and the

EGC CO

064343 Connect the EGC CO

(

2) on the injection valve.

Mixer inlet.

Mixer outlet to port P

Temporary waste container

n/a Collect waste during setup procedures.

(beaker, bottle, etc.) (not supplied) 10-32 to 10-32 coupler

10-32 to 1/4-28 coupler 10-32 fittings

10-32 ferrules 1/8-in fittings

1/8-in ferrules

Doc. 065294-01 3/09 55

042627 042806

043275 043276

052267 048949

Complete the connections.

Page 60

ICS-2100 Installation Instructions

Figure 55 illustrates the EluGen cartridge and EPM after installation.

EluGen

Cartridge

(EGC

EGC Holder

EPM

Figure 55. ICS-2100 with an EluGen Cartridge and EPM Installed

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Setting Up the Eluent Generator

Figure 56 illustrates the liquid flow path through the eluent generator

components after all setup steps are complete and the eluent generator is ready for operation.

EGC VENT

EGC II KCO

Send to waste

TO PUMP/

DAMPER

TO INJ

ALVE IN - P/

ELUENT OUT

Pre-plumbed

Pump Pulse

Damper

*Add backpressure coils, as needed, to create a system backpressure of between 14 and 16 MPa (2000 and 2300 psi).

TO DEGAS ELUENT IN

TO DEGAS

ELUENT OUT

EGC CO

Mixer

TO EGC CO3

MIXER IN

Inlet

EGC

(yellow

label)

TO EGC CO3

MIXER OUT

EGC OUT

EGC

DEGAS

TO INJ VALVE

PORT P

Connect

Inject Valve

Port P (2)

Outlet

(blue

label)

Back-

pressure

Coil*

TO CR-TC/EPM

ELUENT OUT (yellow label)

(

label)

(red

)

ELUENT IN

EPM

TO CR-TC/EPM

REGEN IN

(orange label)

TO SRS/AES

REGEN OUT

Connect to

Suppressor

en Out

)

(

WASTE,

GAS

SEPARATOR

Connect to

Gas Separato

Assembly

Figure 56. Eluent Generator Flow Schematic with an EPM and EGC CO3 Mixer:

Use for Generating Carbonate/Bicarbonate Eluent

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ICS-2100 Installation Instructions

Installing the EluGen Cartridge

1. Tilt the cartridge holder to view the bottom and brace the holder against the back corner of the ICS-2100 (see Figure 57

Figure 57. Cartridge Holder (Tilted to View the Bottom)

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Setting Up the Eluent Generator

2. Use the precut tubing assemblies included in the ICS-2100 Ship Kit to extend the length of three lines as described below:

a. Disconnect the

coupler that connects it to the label). Connect a 32 to 10-32 coupler to the end of the

ELUENT IN line and connect the 30 cm (12 in) length of black

TO CR-TC ELUENT IN line (red label) from the

TO CR-TC ELUENT OUT line (yellow

TO CR-TC

PEEK tubing (red labels) (P/N 064334) to the coupler (see

Figure 58

b. Connect the 35 cm (14 in) length of black PEEK tubing (yellow

labels) (P/N 064340) to the coupler on the

TO CR-TC ELUENT OUT

line (yellow label).

c. Disconnect the

coupler that connects it to the label). Connect (blue labels) (P/N 064337) to the coupler on the

OUT line (blue label).

TO CR-TC REGEN IN line (orange label) from the

TO CR-TC REGEN OUT line (blue

the 28 cm (11 in) length of clear PTFE tubing

TO CR-TC REGEN

TO CR-TC/EPM ELUENT OUT Line (yellow label)

TO CR-TC/EPM

ELUENT IN

Line (red label)

TO CR-TC/EPM REGEN OUT Line (blue label)

Figure 58. EPM Eluent and Regen Line Extensions

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ICS-2100 Installation Instructions

3. Pull the TO CR-TC REGEN IN line (orange label) out of the opening on the side of the holder.

4. Thread the three extended lines (red, yellow , and blue labels) thro ugh the opening on the side of the holder (see Figure 59

TO CR-TC/EPM

ELUENT IN

(red label)

TO CR-TC/EPM

REGEN OUT

(blue label)

TO CR-TC/EPM ELUENT OUT (yellow label)

Figure 59. TO CR-TC/EPM Lines Outside of Holder

5. Turn the EGC holder right-side up and set it back into position.

6. Orient the EluGen cartridge with the fittings facing up and remove the plugs from the cartridge

INLET and OUTLET

fittings (see Figure 60

OUTLET

INLET

Figure 60. EluGen Cartridge Fitting Plugs

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Page 65

Setting Up the Eluent Generator

7. Set the EluGen cartridge in the service area on top of the ICS-2100 and orient it with the cable toward the EGC holder (see Figure 61

EGC Cable

Figure 61. EluGen Cartridge in Service Area

8. Pull the coiled black EGC holder. Disconnect the connects it to the

9. Connect the

EGC IN

EGC IN and EGC OUT lines out from inside the

EGC IN line from the coupler that

EGC OUT line.

line to the EluGen cartridge (see Figure 62

INLET port

10. Remove the coupler and connect the

OUT line to the EluGen

cartridge

Figure 62. EluGen Cartridge Inlet and Outlet Port Connections

EGC

OUTLET port.

11. Turn the EluGen cartridge over (with the fittings facing down). Shake the EluGen cartridge vigorously , and tap it with the palm of your

Doc. 065294-01 3/09 61

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ICS-2100 Installation Instructions

hand 10 to 15 times. Check that all bubbles trapped in the eluent generation chamber are dislodged.

The EluGen cartridge contains one of the following: a corrosive bas e (KOH, LiOH, or NaOH), a corrosive acid (MSA), or a concentrated

solution. Wear protective eyewear and gloves when ha ndling

2CO3

the cartridge. La cartouche d'EluGen contient un de ce qui suit: une base corrosive

(KOH, LiOH, ou NaOH), un acide corrosif (MSA), ou une solution concentrée de K

manipulant la cartouche. Die EluGen-Kassette enthält eine korrodierende Base (KOH, LiOH

oder NaOH), eine korrodierende Säure (MSA) oder eine konzentrierte

-Lösung. Tragen Sie daher beim Umgang mit Kassette eine

2CO3

Schutzbrille und Handschuhe.

12. Slide the EluGen cartridge down into the holder until it stops (see Figure 63 sure the cable remains out of the holder.

. Porter des lunettes et des gants protectives en

2CO3

). Make

NOTE To avoid crimping the lines, do not

twist the cartridge when you slide it into the holder. The lines are designed to coil inside the holder.

Figure 63. EluGen Cartridge Installed in the Holder

62 Doc. 065294-01 3/09

Page 67

13. Remove the plug from

EGC-1 connector on

the top of the ICS-2100.

14. Orient the cartridge’s blue 4-pin electrical cable connector as shown in Figure 64 Push it onto the

EGC-1

connector on the ICS-

2100. Twist the ring on the cable connector fingertight to secure it.

Figure 64. Connecting the EluGen Cartridge Cable

Setting Up the Eluent Generator

Connecting the EluGen Cartridge Vent Line

1. Locate the EluGen Cartridge Vent Line Tubing Kit supplied with the EluGen cartridge.

2. Slide the label onto one end of the tubing and then push the barbed luer lock onto the tubing.

3. Unscrew and remove the plug from the vent opening on the side of the EluGen cartridge (see Figure 65

Figure 65. EluGen Cartridge Vent Plug

Vent Plug

(remove)

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ICS-2100 Installation Instructions

4. Screw the luer adapter onto the vent opening.

Luer Adapter

Luer Lock

Remove the cap on the luer adapter by twisting. Save the cap.

EGC Vent Line

5. Twist the luer lock and EluGen cartridge vent line into the luer adapter (see

Figure 66

Figure 66. Installing the EluGen Cartridge Vent Line

NOTE To prevent leaks from the EluGen cartridge,

always replace the luer adapter cap before moving the EluGen cartridge.

6. Direct the vent line to the waste container and insert it down into the waste, gas separator tube about 10 to 15 cm (4 to 6 in).

Installing the EPM

1. Remove the plugs from

EPM

the EPM eluent and regen liquid ports.

2. Push the EPM onto the

Mounting Bracket

EGC Cable

ball studs on the mounting bracket (see

Figure 67

64 Doc. 065294-01 3/09

Ball Studs

Figure 67. EPM and Mounting Bracket

Page 69

Setting Up the Eluent Generator

3. Lift the EluGen cartridge up slightly from the EGC holder and slide the EPM bracket onto the side of

EGC Cable

the holder (see

Figure 68

Figure 68. EPM and Mounting Bracket Installed on EGC Holder

4. Connect the following lines to the EPM:

• Connect the TO CR-TC/EPM ELUENT OUT line (yellow label) to

the

ELUENT OUT port on the EPM (see Figure 69).

• Connect the TO CR-TC/EPM ELUENT IN line (red label) to the

ELUENT IN port on the EPM.

• Connect the TO CR-TC/EPM REGEN IN line (orange label) to the

REGEN IN port on the EPM.

• Connect the TO CR-TC/EPM REGEN OUT line (blue label) to the

REGEN OUT port on the EPM.

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ICS-2100 Installation Instructions

EGC II KCO

EGC VENT

Send to waste

TO PUMP/

DAMPER

Pre-plumbed

Pump Pulse

Damper

TO DEGAS

ELUENT IN

TO DEGAS

ELUENT OUT

TO INJ VALVE IN - P/ ELUENT OUT

Pre-plumbed

Inject Valve

Port P

Inlet

(yellow

label)

EGC

Outlet

EGC

OUT

EGC

DEGAS

28 cm (11 in) of clear

1.58-mm (0.062-in) ID Teflon tubing (P/N 064337)

35 cm (14 in) of black

0.25-mm (0.010-in) ID PEEK tubing (P/N 064340)

(red

label)

TO CR-TC/EPM

ELUENT OUT (yellow label)

)

(

TO CR-TC/EPM

REGEN IN

(orange label)

(blue

label)

TO SRS/AES

REGEN OUT

30 cm (12 in) of black

0.25-mm (0.010-in) ID PEEK tubing (P/N 064334)

ELUENT IN

(

EPM

I a

Figure 69. EluGen Cartridge and EPM Plumbing with Tubing Extensions

5. Remove the plug from the EGC-2 connector on top of the ICS-2100.

)

WASTE,

GAS

SEPARATOR

Connect to

Gas Separator

Assembl

6. Connect the EPM electrical cable to the

66 Doc. 065294-01 3/09

EGC-2 connector.

Page 71

Setting Up the Eluent Generator

Recording the EluGen Cartridge and EPM Serial Numbers in Chromeleon

The serial numbers are printed on labels on the EluGen cartridge and EPM.

1. Open the Server Configuration program (select Start > Programs > Chromeleon > Server Configuration ).

2. Double-click the ICS-2100 system icon. The properties dialog box for the ICS-2100 appears.

3. Click the Eluent Generator tab (see Figure 70

4. Select the EGC-1 check box and enter the EluGen cartridge serial number.

5. Select the EGC-2 check box and enter the serial number of the EPM.

6. Click OK.

Figure 70. Eluent Generator Configuration Properties: CO3 / EPM Example

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ICS-2100 Installation Instructions

Plumbing the EluGen Cartridge and EPM for Conditioning

Figure 71 illustrates the liquid flow path through the EluGen cartridge and EPM during the conditioning procedure.

EGC II KCO

EGC VENT

Send to waste

TO PUMP/

DAMPER

Pre-plumbed

Pump Pulse

Dam

ELUENT IN

ELUENT OUT

TO INJ VALVE IN - P/ ELUENT OUT

TO DEGAS

Inlet

EGC

(yellow

label)

14 MPa (2000 psi)

at 1.0 mL/min

EGC

OUT

EGC

DEGAS

Back-

pressure

Coil

Outlet

(blue label)

10-32 to

1/4-28 Coupler

(

(red

label)

TO CR-TC/EPM

ELUENT OUT (yellow label)

)

TO SRS/AES REGEN OUT

ELUENT IN

EPM

TO CR-TC/EPM

REGEN IN

(orange)

(

Figure 71. EluGen Cartridge and EPM Plumbing for Conditioning with

Carbonate/Bicarbonate Eluent

)

WASTE,

GAS

SEPARATOR

Connect to

Gas Separator

Assembly

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Page 73

Setting Up the Eluent Generator

1. Disconnect the TO INJ VALVE IN - P/ELUENT OUT line from port P (2) on the injection valve.

2. Use a 10-32 to 10-32 coupler (P/N 042627) to connect the yellow

0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) to the

TO INJ VALVE IN - P/ELUENT OUT line.

3. Connect a 10-32 to 1/4-28 coupler (P/N 042806) to the free end of the backpressure coil.

TO SRS/AES REGEN OUT

10-32 to

1/4-28

Coupler

TO INJ

VALVE IN - P/

ELUENT OUT

4. Disconnect the

REGEN OUT port.

5. Connect the coupler (see Figure 72

TO SRS/AES REGEN OUT line from the suppressor

TO SRS/AES REGEN OUT line to the 10-32 to 1/4-28

NOTE The backpressure coil connection in Figure 72 is

temporary; it is required only during the EPM and EluGen cartridge conditioning procedure described in the next section.

Backpressure Coil

10-32 to 10-32 Coupler

Figure 72. Backpressure Coil Connections for Conditioning the

EluGen Cartridge and EPM with Carbonate/Bicarbonate Eluent

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ICS-2100 Installation Instructions

Conditioning the EPM and EluGen Cartridge

After completing the plumbing connections described in the previous sections, follow the steps below to hydrate the EPM for 5 minutes and then condition the EPM and EluGen cartridge for 30 minutes.

1. Make sure the eluent reservoir is filled with ASTM filtered, Type I (18-megohm) deionized water.

2. Verify that the suppressor is off.

To avoid damaging the suppressor, always turn off the suppressor before conditioning the EPM and EluGen cartridge. The pump flow is on during conditioning, however, no flow reaches the suppressor.

3. Verify that the selected on the ICS-2100 touch screen

Automatically turn on with pump option is not

SUPPRESSOR page. Disabling

this option lets you turn on the pump, without automatically turning on the suppressor.

4. On the ICS-2100 touch screen

HOME page, set the pump flow rate to

1.00 mL/min.

5. Turn on the pump.

6. Run the pump for 5 minutes to hydrate the EPM.

7. Touch the

GENERATOR

8. Verify that the cartridge type for is

pH Mod.

EGC button on the HOME page to open the ELUENT

page.

EGC-1 is Carb and the type for EGC-2

If the cartridge types are wrong, make sure the cartridge serial numbers were entered correctly in the Chromeleon Server Configuration program (see “Recording the EluGen Cartridge and

EPM Serial Numbers in Chromeleon” on page 67).

9. Select the following settings:

• Set the EGC-1 concentration to 9mM.

• Set the EGC-2 concentration to 1mM.

10. Turn on the EGC-1 and EGC-2 currents.

11. Run the EluGen cartridge and EPM at these settings for 30 minutes.

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Page 75

Setting Up the Eluent Generator

12. Turn off the EGC-1 and EGC-2 current.

13. Turn off the pump.

Installing the EGC CO3 Mixer

Figure 73 illustrates the EGC CO3 Mixer after installation.

TO EGC CO3

MIXER OUT

(P/N 064343)

TO EGC CO3

MIXER IN

(P/N 064346)

Figure 73. ICS-2100 with an EGC CO3 Mixer Installed

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ICS-2100 Installation Instructions

1. Use a Phillips head screwdriver to remove the existing small diameter mounting clips from the inside of the ICS-2100 door.

2. Install the larger diameter clips (P/N

063642) (see Figure 74

Figure 74. Location of Mounting Clips for the EGC CO3 Mixer

3. Orient the mixer with the outlet pointing upward and push it onto the clips (see Figure 75 outlet must point upward to ensure thorough mixing of the eluent.

). The mixer

Mixer

Mounting

Clips

Mixer Outlet

4. Locate the 35 cm (14 in) length of black PEEK tubing (white labels) (P/N 064346). Connect the end labeled

TO EGC CO3 MIXER IN to the

mixer inlet.

5. Locate the 60 cm (24 in) length of black PEEK tubing (white labels) (P/N 064343). Connect the end labeled

TO EGC CO3 MIXER OUT to

Mixer Inlet

the mixer outlet.

Figure 75. EGC CO3 Mixer Installed on the ICS-2100 Door

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Page 77

Setting Up the Eluent Generator

Filling the EGC CO

Mixer with Deionized Water

1. Verify that the EGC-1 and EGC-2 currents are off.

2. Disconnect the EluGen cartridge inlet line (labeled

PUMP/DAMPER) from the coupler that connects it to the green pump

outlet line (see Figure 76

TO PUMP/

DAMPER

(disconnect

this line)

Pump Outlet

(green

tubing)

Figure 76. EluGen Cartridge Inlet and Pump Outlet

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ICS-2100 Installation Instructions

3. Connect the pump outlet to the mixer inlet (see Figure 77).

Figure 77. Pump Outlet Connected to Mixer Inlet for Mixer Hydration

4. Direct the mixer outlet line to a temporary waste container, such as a beaker.

Pump Outlet

Mixer Inlet

5. Verify that the suppressor is off.

To avoid damaging the suppressor, always turn off the suppressor before filling the mixer. The pump flow is on during filling, however, no flow reaches the suppressor.

6. Verify that the selected on the ICS-2100 touch screen

Automatically turn on with pump option is not

SUPPRESSOR page. Disabling

this option lets you turn on the pump, without automatically turning on the suppressor.

7. Set the pump flow rate to 5.00 mL/min.

8. Turn on the pump.

9. Run the pump until the mixer is filled and there is a consistent flow of water exiting the mixer outlet.

It takes about 5 minutes to fill a 4-mm mixer and about 2 minutes to fill a 2-mm mixer.

10. Turn off the pump.

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Page 79

Setting Up the Eluent Generator

Filling the EGC CO

Mixer with the Carbonate/Bicarbonate Eluent

Before initial use, follow the steps in this section to fill the EGC CO3 Mixer with the carbonate/bicarbonate eluent concentration required for the application to be run.

Figure 78

components during initial filling of the EGC CO

illustrates the liquid flow path through the eluent generator

Mixer with

carbonate/bicarbonate eluent.

EGC VENT

EGC II

KCO

Send to waste

TO PUMP/

DAMPER

ELUENT OUT

TO INJ

ALVE IN - P/

ELUENT OUT

TO DEGAS ELUENT IN

TO DEGAS

EGC CO

Inlet

(yellow)

TO EGC CO3

MIXER OUT

Mixer

EGC

DEGAS

OUT

Outlet

(blue)

TO CR-TC/EPM

ELUENT OUT

(

(red)

(yellow)

U )

TO CR-TC/EPM

ELUENT IN

EPM

REGEN IN

(orange)

)

(

WASTE,

GAS

SEPARATOR

Pre-plumbed

Pump Pulse

Damper

* 14 MPa (2000 psi) at 1.0 mL/min

Back-

Pressure

Coil*

TO EGC CO3

MIXER IN

TO INJ VALVE

PORT P

TO SRS/AES REGEN OUT

Connect to

Gas Separato

Assembly

Figure 78. EluGen Cartridge and EPM Plumbing for Initial Filling

of the EGC CO

Doc. 065294-01 3/09 75

Mixer with Carbonate/Bicarbonate Eluent

Page 80

ICS-2100 Installation Instructions

1. Disconnect the pump outlet from the mixer inlet.

2. Reconnect the pump outlet and the EluGen cartridge inlet.

3. Connect a 10-32 to 10-32 coupler to the mixer inlet.

4. Disconnect the outlet of the yellow PEEK backpressure coil from the

TO SRS/AES REGEN OUT line and then connect the backpressure coil

to the mixer inlet (see Figure 79

5. Connect the mixer outlet to the

NOTE The backpressure coil connection is temporary;

it is required only during initial filling of the mixer.

pressure

TO SRS/AES REGEN OUT line.

Mixer Outlet

TO SRS/AES REGEN OUT

Back-

Coil

Mixer Inlet

TO INJ PORT IN - P/ELUENT OUT

Figure 79. Mixer Connected Directly to Eluent

Generator Outlet for Initial Filling

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Setting Up the Eluent Generator

6. Verify that the suppressor is off.

To avoid damaging the suppressor, always turn off the suppressor before filling the mixer. The pump flow is on during filling, however, no flow reaches the suppressor.

7. Verify that the selected on the ICS-2100 touch screen

Automatically turn on with pump option is not

SUPPRESSOR page. Disabling

this option lets you turn on the pump, without automatically turning on the suppressor.

8. Set the pump flow rate to the rate required for the application.

9. Turn on the pump.

10. Verify that the system backpressure is between 14 and 16 MPa (2000 and 2300 psi). If necessary, adjust the pressure by adding or removing backpressure tubing.

11. Set the your application. For example, for a 3.5 mM K

mixture, set the

EGC-1 and EGC-2 concentrations to the values required for

/1.0 mM KHCO3

2CO3

EGC-1 concentration to 3.5 mM and the EGC-2

concentration to 1.0 mM.

12. Turn on the

EGC-1 and EGC-2 currents.

13. Run at the selected settings until the mixer is filled with the eluent mixture. At a flow rate of 1 mL/min it will take about 45 minutes to fill the 4-mm mixer and about 15 minutes to fill the 2-mm mixer.

NOTE The void volume of the 4-mm mixer is about

16 mL. The void volume of the 2-mm mixer is about 5 mL.

14. Turn off the EGC-1 and EGC-2 currents.

15. Turn off the pump.

16. Remove the yellow PEEK backpressure coil from the

IN - P/ELUENT OUT

17. Disconnect the mixer outlet from the

line and the mixer inlet.

TO SRS/AES REGEN OUT line

TO INJ VALVE

and remove the 10-32 to 1/4-28 coupler.

18. Reconnect the

port.

OUT

Doc. 065294-01 3/09 77

TO SRS/AES REGEN OUT line to the suppressor REGEN

Page 82

ICS-2100 Installation Instructions

Completing the Eluent Generator Setup for Carbonate/Bicarbonate Eluent

Figure 80 illustrates the liquid flow path through the ICS-2100 after all

setup steps are complete and the eluent generator is ready for operation.

To the pump Degas In

EGC II K

2CO3

Inlet Outlet

Eluent Out

Deionized

Water

EGC Vent

Cell

Pump Head

Eluent

Valve

Regen Out

Press.

Xducer.

Eluent In

Suppressor

Regen Out Eluent Out

Pump

Head

EPM

Regen In

Eluent In Regen In

Column

Heater

Heat Exchanger

Guard

Back-

pressure

Coil

Separator

EGC CO

Mixer

To wast

To Gas Separator Assembly

EGC

Degas

Degas In

Degas Out

Conductivity

Cell In

Cell Out

Pulse Damper

Valve

Sample in

To waste

Figure 80. ICS-2100 Flow Schematic: Carbonate/Bicarbonate Eluent Generation

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Setting Up the Eluent Generator

1. Connect the TO INJ VALVE IN - P/ELUENT OUT line to the coupler attached to the mixer inlet line. Route this line along the back of the drip tray to prevent it from being pinched when the ICS-2100 door is opened and closed

2. Route the mixer outlet line to the back of the component panel (see

Figure 81

3. Connect the mixer outlet line to port

Figure 81. Mixer Outlet Routed to the Back of the Component Panel

NOTE Backpressure tubing may be required between the

injection valve and the EGC CO EluGen cartridge requires 14 to 16 MPa (2000 to 2300 psi) of system backpressure for removal of electrolysis gas from the eluent produced by the cartridge. See Section 19.1

P (2) on the injection valve.

Mixer outlet. The

for details.

4. Go on to Section 19, “Equilibrating the System.”

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ICS-2100 Installation Instructions

18.3 Eluent Generator Setup for Carbonate Eluent

Follow the instructions in this section if you are installing a K2CO3 cartridge and an EGC CO

Mixer (for generating carbonate eluent).

• If you are installing a KOH, LiOH, MSA, or NaOH EluGen cartridge

and a CR-TC, go to Section 18.1

• If you are installing a K

cartridge, an EPM, and an EGC CO

2CO3

Mixer (for generating carbonate/bicarbonate eluent), go to

Section 18.2

Overview

The eluent generator setup procedure for carbonate eluent consists of the following main steps:

• Installing the EluGen cartridge and preparing for conditioning.

• Conditioning the EluGen cartridge.

• Installing the EGC CO

• Filling the EGC CO

the application.

Mixer.

Mixer with the eluent concentration required for

• Completing the eluent generator setup.

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Setting Up the Eluent Generator

Items Required to Complete the Setup Procedure

In addition to the EluGen cartridge and the EGC CO3 Mixer, you will need the following items to complete the eluent generator setup. Except as noted, all items are supplied in the ICS-2100 Ship Kit (P/N 064375).

Item P/N Use To

Yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil

EluGen Cartridge Vent Line Tubing Kit (supplied with the cartridge)

EGC CO

Mixer mounting

clips Phillips head screwdriver (not

supplied) 35 cm (14 in) of black

0.25-mm (0.010-in) ID PEEK tubing

60 cm (24 in) of black

0.25-mm (0.010-in) ID PEEK tubing

Temporary waste container (beaker, bottle, etc.) (not supplied)

10-32 fittings 10-32 ferrules

1/8-in fittings 1/8-in ferrules

053765 Provide backpressure during

setup procedures.

063092 Vent the cartridge during

operation.

063642 Install the mixer.

n/a Install the EGC CO

Mixer

mounting clips.

064346 Connect the EGC degas eluent

outlet and the EGC CO

Mixer

inlet.

064343 Connect the EGC CO

outlet to port

P (2) on the

Mixer

injection valve.

n/a Collect waste during setup

procedures.

043275 043276

052267 048949

Complete the plumbing connections.

10-32 to 10-32 couplers 042627 Complete the plumbing

connections.

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ICS-2100 Installation Instructions

Figure 82 illustrates the liquid flow path through the eluent generator

components after all setup steps are complete and the eluent generator is ready for operation.

EGC II

KCO

EGC VENT

Send to waste

TO PUMP/

DAMPER

Pre-plumbed

Pump Pulse

Damper

TO INJ

VALVE IN - P

ELUENT OUT

TO DEGAS ELUENT IN

TO DEGAS

ELUENT OUT

EGC CO

Mixer

TO EGC CO3

MIXER IN

Inlet

EGC

Outlet

EGC OUT

EGC

DEGAS

TO EGC CO3

MIXER OUT

Back-

pressure

Coil*

TO INJ VALVE

PORT P

Connect

Inject Valve

Port P (2)

*Add backpressure coils, if needed, to create a system backpressure of between 14 and 16 MPa (2000 and 2300 ps i).

TO SRS/AES REGEN OUT

Connect to

Suppressor

Regen Out

WASTE,

GAS

SEPARATOR

Connect to

Gas Separato

Assembly

Figure 82. Eluent Generator Flow Schematic with an EGC CO3 Mixer:

Use for Generating Carbonate Eluent

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Page 87

Setting Up the Eluent Generator

Installing the EluGen Cartridge and Preparing for Conditioning

Follow the steps in this section to install the EluGen cartridge and prepare it for conditioning before initial use.

Figure 83

procedure.

illustrates the liquid flow path during the conditioning

EGC VENT

EGC II

KCO

Send to waste

EGC

EGC OUT

Outlet

TO DEGAS ELUENT IN

TO DEGAS

ELUENT OUT

Inlet

EGC

DEGAS

TO PUM P/

DAMPER

Pre-plumbed

Pump Pulse

Damper

TO INJ

VALVE IN - P/

ELUENT OUT

Back-

pressure

Coil

14 MPa (2000 psi) at 1.0 mL/min

To waste

TO SRS/AES

REGEN OUT

Connect to

Suppressor

Regen Out

SEPARATOR

Connect to

Gas Separato

Figure 83. EluGen Cartridge Plumbing for Conditioning with Carbonate Eluent

Doc. 065294-01 3/09 83

WASTE,

GAS

Assembly

Page 88

ICS-2100 Installation Instructions

1. Orient the EluGen cartridge with the fittings facing up and remove the plugs from the cartridge

INLET and OUTLET

fittings (see Figure 84

2. Set the EluGen cartridge in the service area on top of the ICS-2100 and orient it with the cable toward the EGC holder (see Figure 85

INLET

Figure 84. EluGen Cartridge Fitting Plugs

OUTLET

EGC Cable

Figure 85. EluGen Cartridge in Service Area

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Page 89

Setting Up the Eluent Generator

3. Pull the coiled black EGC IN and EGC OUT lines out from inside the EGC holder. Disconnect the connects it to the

EGC OUT line.

EGC IN line from the coupler that

4. Connect the

EGC IN

line to the EluGen cartridge (see Figure 86

INLET port

5. Remove the coupler and connect the

line to the EluGen

OUT

cartridge

EGC

OUTLET port.

Figure 86. EluGen Cartridge Port Connections

6. Turn the EluGen cartridge over (with the fittings facing down). Shake the EluGen cartridge vigorously , and tap it with the palm of your hand 10 to 15 times. Check that all bubbles trapped in the eluent generation chamber are dislodged.

The EluGen cartridge contains one of the following: a corrosive bas e (KOH, LiOH, or NaOH), a corrosive acid (MSA), or a concentrated

solution. Wear protective eyewear and gloves when handling

2CO3

the cartridge. La cartouche d'EluGen contient un de ce qui suit: un e base corrosive

(KOH, LiOH, ou NaOH), un acide corrosif (MSA), ou une solution concentrée de K

manipulant la cartouche. Die EluGen-Kassette enthält eine korrodierende Base (KOH, LiOH

oder NaOH), eine korrodierende Säure (MSA) oder eine konzentrie rte

-Lösung. Tragen Sie daher beim Umgang mit Kassette eine

2CO3

Schutzbrille und Handschuhe.

. Porter des lunettes et des gants protectives en

2CO3

Doc. 065294-01 3/09 85

Page 90

ICS-2100 Installation Instructions

7. Slide the EluGen cartridge down into the holder until it stops (see Figure 87 sure the cable remains out of the holder.

NOTE To avoid crimping the lines, do not

twist the cartridge when you slide it into the holder. The lines are designed to coil inside the holder.

Figure 87. EluGen Cartridge Installed in the Holder

8. Remove the plug from

EGC-1 connector on

the top of the ICS-2100.

). Make

9. Orient the cartridge’s blue 4-pin electrical cable connector as shown in Figure 88 Push it onto the

EGC-1

connector on the ICS-

2100. Twist the ring on the cable connector fingertight to secure it.

Figure 88. Connecting the EluGen Cartridge Cable

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Page 91

Setting Up the Eluent Generator

10. Disconnect the TO INJ VALVE IN - P/ELUENT OUT line from port P (2) on the injection valve.

11. Use a 10-32 to 10-32 coupler (P/N 042627) to connect the

VALVE IN - P/ELUENT OUT

line to one end of the yellow 0.5 mL/min,

TO INJ

7 MPa (1000 psi) backpressure coil (P/N 053765) (see Figure 89

12. Direct the free end of the backpressure coil to a temporary waste container.

EGC Cable

TO INJ V AL VE

IN - P/

ELUENT OUT

Back-

pressure

Coil

Temporary

Waste

Container

Figure 89. Plumbing Connections for Conditioning the

EluGen Cartridge with Carbonate Eluent

Installing the EluGen Cartridge Vent Line

1. Locate the EluGen Cartridge Vent Line Tubing Kit (P/N 063092) supplied with the EluGen cartridge.

2. Slide the label onto one end of the tubing and then push the barbed luer lock onto the tubing.

Doc. 065294-01 3/09 87

Page 92

ICS-2100 Installation Instructions

3. Unscrew and remove the plug from the vent opening on the side of the EluGen cartridge (see Figure 90

Vent Plug

(remove)

Figure 90. EluGen Cartridge Vent Plug

4. Screw the luer adapter onto the vent opening.

Luer Adapter

Luer Lock

Remove the cap on the luer adapter by twisting. Save the cap.

EGC Vent Line

5. Twist the luer lock and EluGen cartridge vent line into the luer adapter (see

Figure 91

NOTE To prevent leaks from the EluGen cartridge, always

Figure 91. Installing the EluGen Cartridge Vent Line

replace the luer adapter cap before moving the EluGen cartridge.

6. Direct the EluGen cartridge vent line to the rear of the ICS-2100 and snap it onto one of the tubing clips on the rear panel.

7. Direct the vent line to the waste container and insert it down into the waste, gas separator tube about 10 to 15 cm (4 to 6 in).

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Page 93

Setting Up the Eluent Generator

Recording the EluGen Cartridge Serial Number in Chromeleon

The serial number is printed on the EluGen cartridge label.

1. Open the Chromeleon Server Configuration program (click Start on the Windows taskbar and select All Programs > Chromeleon >

>Server Configuration).

2. Double-click the ICS-2100 system icon. The properties dialog box for the ICS-2100 appears.

3. Click the Eluent Generator tab (see Figure 92

4. Select the EGC-1 check box.

5. Enter the EluGen cartridge serial number and click OK.

Figure 92. Eluent Generator Configuration Properties: CO3 Example

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Page 94

ICS-2100 Installation Instructions

Conditioning the EluGen Cartridge

After completing the installation steps described in the previous sections, condition the EluGen cartridge for 30 minutes before initial use.

1. Make sure the eluent reservoir is filled with ASTM filtered, Type I (18-megohm) deionized water.

2. Verify that the suppressor is off.

To avoid damaging the suppressor, always turn off the suppressor before conditioning the EluGen cartridge. The pump flow is on during conditioning, however, no flow reaches the suppressor.

3. Verify that the selected on the ICS-2100 touch screen

Automatically turn on with pump option is not

SUPPRESSOR page. Disabling

this option lets you turn on the pump, without automatically turning on the suppressor.

4. On the ICS-2100 touch screen

HOME page, set the pump flow rate to

1.00 mL/min.

5. Turn on the pump.

6. On the touch screen

ELUENT GENERATOR page. Verify that the cartridge type is Carb.

HOME page, touch the EGC button to open the

If the cartridge type is wrong, verify that the cartridge serial number was entered correctly in the Chromeleon Server Configuration program (see “Recording the EluGen Cartridge Serial Number in

Chromeleon” on page 89).

7. Set the

EGC-1 concentration to 9mM.

8. Turn on the EGC-1 current.

9. Run the EluGen cartridge at these settings for 30 minutes.

10. Turn off the EGC-1 current.

11. Turn off the pump.

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Page 95

Setting Up the Eluent Generator

Installing the EGC CO

Mixer

Figure 93 illustrates the EGC CO3 Mixer after installation.

TO EGC CO3

MIXER OUT

(P/N 064343)

TO EGC CO3

MIXER IN

(P/N 064346)

Figure 93. ICS-2100 with an EGC CO3 Mixer Installed

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Page 96

ICS-2100 Installation Instructions

1. Use a Phillips head screwdriver to remove the existing small diameter mounting clips from the inside of the ICS-2100 door.

2. Install the larger diameter mounting clips (P/N 063642) (see Figure 94

Figure 94. Location of Mounting Clips for the EGC CO3 Mixer

3. Orient the mixer with the outlet pointing upward and push it onto the clips (see Figure 95 outlet must point upward to ensure thorough mixing of the eluent.

). The mixer

Mixer

Mounting

Clips

Mixer Outlet

4. Locate the 35 cm (14 in) length of black PEEK tubing (white labels) (P/N 064346). Connect the end labeled

TO EGC CO3 MIXER IN to the

mixer inlet.

5. Locate the 60 cm (24 in) length of black PEEK tubing (white labels) (P/N 064343). Connect the end labeled

TO EGC CO3 MIXER OUT to

Mixer Inlet

the mixer outlet.

Figure 95. EGC CO3 Mixer Installed on the ICS-2100 Door

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Page 97

Setting Up the Eluent Generator

Filling the EGC CO

Mixer with the Carbonate Eluent

Before initial use, follow the steps in this section to fill the EGC CO3 Mixer with the carbonate eluent required for the application to be run.

Figure 96

components during initial filling of the EGC CO

illustrates the liquid flow path through the eluent generator

Mixer with carbonate

eluent.

EGC VENT

EGC II

KCO

Send to waste

Inlet

EGC

Outlet

EGC OUT

TO PUMP/

DAMPER

Pre-plumbed

Pump Pulse

Damper

TO INJ VALVE IN - P/ ELUENT OUT

Back-

pressure

Coil

TO DEGAS ELUENT IN

TO DEGAS

ELUENT OUT

EGC CO

14 MPa (2000 psi) at 1.0 mL/min

DEGAS

Mixer

TO EGC CO3

MIXER IN

EGC

TO EGC CO3

MIXER OUT

To waste

TO SRS/AES REGEN OUT

Connect to

Suppressor

Regen Out

SEPARATOR

Connect to

Gas Separato

Figure 96. Plumbing for Filling the EGC CO3 Mixer with Carbonate Eluent

Doc. 065294-01 3/09 93

WASTE,

GAS

Assembl

Page 98

ICS-2100 Installation Instructions

1. Connect a 10-32 to 10-32 coupler to the mixer inlet and connect the outlet of the yellow PEEK backpressure coil to the coupler.

2. Direct the mixer outlet to the temporary waste container.

3. Verify that the suppressor is off.

To avoid damaging the suppressor and CR-TC, always turn off the suppressor before conditioning the EluGen cartr idge. The pump flow is on during conditioning, however, no flow reaches the suppressor.

4. Verify that the selected on the ICS-2100 touch screen

Automatically turn on with pump option is not

SUPPRESSOR page. Disabling

this option lets you turn on the pump, without automatically turning on the suppressor.

5. Set the pump flow rate to the rate required for the application.

6. Turn on the pump.

7. Verify that the system backpressure is between 14 and 16 MPa (2000 and 2300 psi). If necessary, adjust the pressure by adding or removing backpressure tubing.

8. Set the

EGC-1 concentration to the value required for your

application.

9. Turn on the

EGC-1 current.

10. Run at these settings until a consistent flow of eluent is exiting the mixer outlet. At a flow rate of 1 mL/min it will take about 16 minutes to fill a 4-mm mixer and about 5 minutes to fill a 2-mm mixer.

NOTE The void volume of the 4-mm mixer is about

16 mL. The void volume of the 2-mm mixer is about 5 mL.

11. Turn off the EGC-1 current.

12. Turn off the pump.

13. Remove the yellow PEEK backpressure coil from the

IN - P/ELUENT OUT

line and the mixer inlet.

TO INJ VALVE

14. Retain the coupler on the mixer inlet.

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Setting Up the Eluent Generator

Completing the Eluent Generator Setup for Carbonate Eluent

Figure 97 illustrates the liquid flow path through the ICS-2100 after all

setup steps are complete and the eluent generator is ready for operation.

To the pump Degas In

EGC Vent

EGC II K

2CO3

Deionized

Water

Inlet Outlet

Sample

Valve

To waste

EGC

Degas

Degas In

Degas Out

Conductivity

Cell

Cell In

Cell Out

Pulse Damper

Pump

Head

Eluent

Valve

Press.

Xducer.

Eluent In Regen In

Suppressor

Pump

Head

Regen Out Eluent Out

pressure

Column

Heater

Heat Exchanger

Guard

Back-

Coil

EGC CO

Separator

Mixer

To waste

To Gas

Separator

Assembly

Figure 97. ICS-2100 Flow Schematic: Carbonate Eluent Generation

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ICS-2100 Installation Instructions

2. Route the mixer outlet line to the back of the component panel (see

Figure 98

3. Connect the mixer outlet line to port

Figure 98. Mixer Outlet Routed to the Back of the Component Panel

NOTE Backpressure tubing may be required between the

P (2) on the injection valve.

Mixer outlet. The

for details.

4. Go on to Section 19, “Equilibrating the System.”

96 Doc. 065294-01 3/09

Dionex ICS-2100 Installation Instructions Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Contents

Installation

1. Facility Requirements

2. Unpacking the IC System

3. Connecting an Autosampler (Optional)

3.1 Connecting an AS-DV Autosampler

3.2 Connecting an AS Autosampler

4. Unpacking the PC

5. Installing Chromeleon

6. Installing the Chromeleon Software License Dongle

7. Installing the Software License

8. Starting the Chromeleon Server

9. Connecting to the Chromeleon PC

9.1 Connecting the IC System to the PC

9.2 Connecting Additional USB Devices

10. Connecting the Power Cord

11. Installing the ICS-2100 Device Driver

12. Setting Up Chromeleon

12.1 Creating a Timebase

12.2 Configuring the Device Properties

12.3 Connecting to the Panel Tabset

12.4 Verifying Chromeleon Communication

13. Installing and Plumbing the Columns and Suppressor

13.1 Unpacking the Columns and Suppressor

13.2 Setting Up the Column Heater

13.3 Plumbing the Columns

13.4 Installing the Suppressor

14. Connecting the Waste Lines

14.1 Installing the Waste, Gas Separator Tube

15. Setting Up the Eluent Reservoir

16. Entering the Eluent Level

17. Priming the Pump

17.1 Priming the Eluent Lines with a Syringe

17.2 Priming the Pump Heads with the Prime Button

18. Setting Up the Eluent Generator

18.1 Eluent Generator Setup for KOH, LiOH, MSA, and NaOH Eluents

18.2 Eluent Generator Setup for Carbonate/Bicarbonate Eluent

18.3 Eluent Generator Setup for Carbonate Eluent