Dionex GP50 Operator's Manual

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GP50 GRADIENT PUMP

OPERATOR'S MANUAL

Document No. 031377

Revision 03

July 2001

This publication is protected by federal copyright law. No part of this publication may be copied or distributed, transm itted, tr anscri bed , stored in a retrieval system, or transmitted into any human or computer language, in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties without the express written permission of Dionex Corporation, 1228 Titan Way, Sunnyvale, California 94088-3603 U.S.A.

DISCLAIMER OF WARRANTY AND LIMITED WARRANTY

THIS PUBLICATION IS PROVIDED “AS IS” WITHOUT WARRANT Y OF ANY KIND. DIONEX CORPORATION DOES NOT WARRANT, GUARANTEE, OR MAKE ANY EXPRESS OR IMPLIED REPRESENTATI ONS RE GARD ING THE U SE, O R TH E RES ULTS OF THE USE, OF THIS PUBLICATION IN TERMS OF CORRECTNESS, ACCURACY, RELIABILITY, CURRENTNESS, OR OTHERWISE. FURTHER, DIONEX CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVI SION OR CHANGES.

TRADEMARKS

DX-LAN™ is a trademark, and PeakNet®, Self-Regenerating Suppressor®, and SRS® are registered trademarks of Dionex Corporation Tefzel® is a registered trademark of E.I. duPont de Nemours & Co.

PRINTING HISTORY

Revision 01, May 1998 Revision 02, October 2000 Revision 03, July 2001

1 • Introduction

1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

1.2 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

1.2.1 Safety Messages and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

1.2.2 Safety Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4

2 • Description

2.1 Front Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

2.1.1 Control Panel Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3

2.1.2 Display Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7

2.2 Electronics Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8

2.3 Mechanical Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-11

2.4 Mechanical Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-12

2.5 Vacuum Degas Pump Assembly (Optional) . . . . . . . . . . . . . . . . . . . .2-16

2.6 Piston Seal Wash (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-16

2.7 Eluent Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-17

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2.4.1 Pump Eluent Manifold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-13

2.4.2 Pump Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-14

2.4.3 Pump Mixers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-14

2.4.4 Pump Priming Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-15

2.4.5 Pressure Transducer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-15

GP50 Gradient Pump

2.8 Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-18

2.9 Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-18

2.9.1 Operating and Control Modes . . . . . . . . . . . . . . . . . . . . . . . . 2-18

2.9.2 Local and Remote Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-19

2.9.3 Method Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-20

2.9.4 Eluent Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-22

3 • Operation and Maintenance

3.1 Getting Ready to Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

3.1.1 Degas Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

3.1.2 Filter Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

3.1.3 Pressurize Eluent Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

3.1.4 Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

3.1.5 Selecting the Pressure Limits . . . . . . . . . . . . . . . . . . . . . . . . . 3-4

3.1.6 Selecting the Operating and Control Modes . . . . . . . . . . . . . . 3-5

3.2 Running Under Direct Control (Local Mode) . . . . . . . . . . . . . . . . . . . 3-6

3.3 Running Under Method Control (Local Mode) . . . . . . . . . . . . . . . . . . 3-7

3.3.1 Creating a New Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8

3.3.2 Running a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-10

3.3.3 Editing a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11

3.3.4 Deleting a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12

3.3.5 Changing the Running Method . . . . . . . . . . . . . . . . . . . . . . . 3 -12

3.3.6 Controlling the Method Clock . . . . . . . . . . . . . . . . . . . . . . . .3-12

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3.4 Example Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-13

3.4.1 Isocratic Method Example . . . . . . . . . . . . . . . . . . . . . . . . . . .3-13

3.4.2 Linear Gradient Method Example . . . . . . . . . . . . . . . . . . . . .3-14

3.4.3 Curved Gradient Method Example . . . . . . . . . . . . . . . . . . . . .3-19

3.4.4 Editing a Running Method Example . . . . . . . . . . . . . . . . . . .3-23

3.5 Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-25

3.5.1 Daily Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-25

3.5.2 Periodic Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-27

3.6 Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-27

4 • Troubleshooting

4.1 Left-Right Pump Head Pressure Fluctuations . . . . . . . . . . . . . . . . . . . .4-1

Contents

4.2 Pump Will Not Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3

4.3 Pump Stops . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3

4.4 Liquid Leaks/Leak Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7

4.5 Noisy Pump Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-8

4.6 Vacuum Degas Pump Does Not Run . . . . . . . . . . . . . . . . . . . . . . . . . .4-9

4.7 Vacuum Degas Pump Calibration Fails . . . . . . . . . . . . . . . . . . . . . . .4-10

4.8 Vacuum Degas Pump Low Vacuum . . . . . . . . . . . . . . . . . . . . . . . . . .4-11

4.9 Relays or TTLs Inoperative . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11

4.10 Poor Chromatographic Reproducibility . . . . . . . . . . . . . . . . . . . . . . .4-12

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iii

GP50 Gradient Pump

5•Service

5.1 Cleaning and Replacing the Check Valves . . . . . . . . . . . . . . . . . . . . . . 5-1

5.2 Piston Seal Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4

5.3 Pump Piston Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8

5.4 Pressure Transducer Waste Valve O-Ring Replacement . . . . . . . . . . 5-10

5.5 Proportioning Valve Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . .5-11

5.6 Changing Main Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-13

A • Specifications

A.1 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1

A.2 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1

A.3 Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1

A.4 Display and Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1

A.5 Hydraulics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-2

A.6 Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-3

A.7 Vacuum Degas Pump Assembly (Optional) . . . . . . . . . . . . . . . . . . . . .A-3

B • Installation

B.1 Facility Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .B-1

B.2 Installation Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .B-2

B.2.1 Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .B-2

B.2.2 Electronics Chassis Connections . . . . . . . . . . . . . . . . . . . . . . .B-4

B.2.3 DX-L AN Interface: 10B ASE-T Connections (Optional). . . . .B-6

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B.2.4 DX-LAN Interface: BNC Connections (Optional) . . . . . . . . . B-9

B.2.5 Waste Lines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-13

B.2.6 Eluent Inlet Line Connections. . . . . . . . . . . . . . . . . . . . . . . . B-14

B.2.7 Eluent Outlet Line Connections . . . . . . . . . . . . . . . . . . . . . . B-14

B.2.8 Piston Seal Wash Connections (Optional) . . . . . . . . . . . . . . B-15

B.2.9 Priming the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-17

B.3 Automatic SRS Power Control (Optional) . . . . . . . . . . . . . . . . . . . . B-20

C • Display Screens

C.1 Operational Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2

C.1.1 Menu of Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2

C.1.2 Main Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-3

Contents

C.2 Diagnostic Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-14

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C.1.3 Detail Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-5

C.1.4 Method Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-6

C.1.5 Method Extension Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-8

C.1.6 Degas Options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-9

C.1.7 Module Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-11

C.1.8 Pump Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-12

C.1.9 Time Function In. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-13

C.2.1 Diagnostic Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-14

C.2.2 Power-Up Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-15

C.2.3 Elapsed Time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-16

C.2.4 DSP Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-17

GP50 Gradient Pump

C.2.5 DX-LAN Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-18

C.2.6 Keyboard Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-20

C.2.7 Diagnostic Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-21

C.2.8 Pressure Statistics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-23

C.2.9 DSP Code Version . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-24

C.3 Calibration Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-25

C.3.1 Calibration Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-25

C.3.2 Calibration Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-26

C.3.3 Leak Sensor Calibration and Status . . . . . . . . . . . . . . . . . . . .C-27

C.3.4 Degas Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-28

C.3.5 Flow Calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-29

C.3.6 Pressure Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-30

C.3.7 Degas Pump Calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-34

D • TTL and Relay Control

D.1 TTL and Relay Output Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . .D-2

D.2 TTL Input Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .D-3

D.2.1 TTL Input Signal Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .D-3

D.3 TTL and Relay Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .D-5

D.3.1 Example Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .D-6

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1.1 Overview

The GP50 Gradient Pump is an integral part of a Dione x chr omat ogr aphy system. It is a microprocess or -based, dua l-pisto n, v ariable -speed, gr adient de li ver y system designed to blend and pump mixtures of up to four different eluents at precisely controlled flow rates. The pump can deliver the selected eluent composition isocratically, or as a multistep linear or curved gradient. A Digital Signal Processor (DSP) provides high speed control of pump flow and pressure.

The pump can operate as a stand-alone product or with other Dionex modules as part of a complete chromatography system. It can also be used with non-Dionex modules that meet interface requirements for software, TTL, or relay control.

The GP50 can be controlled locally, using the front panel keypad and display, or from a remote host computer with a Dionex DX-LAN™ interface installed and

PeakNet ava ilabl e from an y de vi ce capabl e of pro v iding compati ble TTL signa ls to contr ol the pump.

The pump's two basic mode s of control, Dire ct control and Method control, e nable it to operat e with or without reference to time-based events.

software installed on t he hos t comput er. Limited remote control is also

1 • Introduction

The GP50 is available in four versions. An optional vacuum degas pump is available for all versions:

GP50 Gradient Pump V e r sio n With Degas Pump Without Degas Pump

Standard bore with PEEK components

Standard bore with stainless steel components

Microbore with PEEK components P/N 054045 P/N 054429 Microbore with stainless steel

components

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P/N 054426 P/N 054427

P/N 054419 P/N 054420

P/N 054425 P/N 054424

1-1

GP50 Gradient Pump

1.2 About This Manual

Chapter 1

Introduction

Chapter 2

Description

Chapter 3

Operation and

Maintenance

Chapter 4

Troubleshooting

Chapter 5

Service

Appendix A

Specifications

Appendix B

Installation

Appendix C

Display Screens

Appendix D

TTL and Relay

Control

Gives an overview of the GP50 Gradient Pump, and explains conventions used in this manual, including safety-related information.

Describes the GP50 front panel controls, electronic and mechanical components, and operating modes.

Provides an overview of GP50 opera tion, including how to create, edit, and run methods from the GP50 front panel. Lists routine preventive maintenance procedures.

Lists problems and presents step-by-step procedures for how to isolate and eliminat e them.

Provides step-by-step instructions for routine service and parts replacement procedures.

Lists the GP50 specifications and installation site requirements.

Describes how to install the GP50.

Illustrates and describes all of the screens that can be displayed on the GP50 front panel.

Describes relay and TTL input and output functions and provides installation instructions

1-2

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1.2.1 Saf et y Me ss a ges and Note s

This manual contains warnings and precautionary statements that, when properly followed, can prevent personal injury and/or damage to the GP50. Safety messages appear in bold type and are accompanied by icons, as shown below.

Indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.

Indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

Indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury.

1 • Introduction

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Indicates that the function or process of the instrument may be impaired. Operation does not constitute a hazard.

Informational messages also appear throughout this manual. These are labeled NOTE and are in bold type:

NOTE NOTES call attention to certain information. They alert

you to an unexpected result of an action, suggest how to optimize instrument performance, etc.

1-3

GP50 Gradient Pump

1.2.2 Saf et y Label s

The TUV GS, C, US Mark safety label and the CE Mark label on the GP50 indicate that the GP50 is in compliance with the following standards: EN 61010-1:1993 (safety), CAN/CSA-C22.2 No. 1010.1-92 (safety), UL 3101-1/10.93 (safety), EN 50082-1:1992 (susceptibility), and EN 55011:1991 (emissions).

The symbols below appear on the GP50, or on GP50 labels.

Alternating current

Protective conductor terminal

Power supply is on

Power supply is off

1-4

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2 • Description

The GP50 Gradient Pump consists of two units (see Figure 2-1). The upper unit houses the electronics components and the lower unit houses the pump heads and other mechanical pump assemblies.

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Pump Heads

Figure 2-1. GP50 Enclosure

2-1

GP50 Gradient Pump

2.1 Front Control Panel

The control panel on the upper door of the pump enclosure contains a liquid crystal display (LCD), a membrane keypad, and the actuator for the main power switch (see Figure 2-2). The door opens to provide access to the electronics chassis (see Section 2.2).

NOTE If no keypad buttons are pressed within a two-hour

period, the front panel backlight will automatically turn off. To restore the backlight, press any button.

Screen Contrast

Information is displayed on the LCD, also called the contrast, use the knurled knob in the recess below the keypad (see Figure 2-2).

Tilt Panel

To maximize visibility, the front control panel can be tilted to four different positions. To tilt the panel, support the door at the left side (to prevent it from opening) and lift firmly on the tab in the middle of the recess below the keypad (see Figure 2 -2). Push on the tab t o return the panel to its vertical position.

. To adjust the screen

screen

2-2

Power Switches

The main powe r swit ch is on the bulkhead behind the uppe r door (see Figur e 2-1). An actuator for the main power switch is on the outside of the front door, at the lower left corner (see Figure 2-2).

The actuator functio ns only when the door is fully clos ed. Whe n the door is open, press the main power switch on the bulkhead to turn the module off and on.

To prevent damage to the pump circuitry and components, always wait at least 15 seconds after powering down before turning on the power again.

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GP50 G radient Pump

100.0 %A

0.0 %B

0.0 %C

0.0 %D

Help Message

LOAD

COLUMN A

2000

2.00

LOCAL 2

10.27

METHOD

2•Description

PSI m L /MIN

MIN

Off/O n

Prime Reset

Insert

Delete

Ma in P o wer Sw itc h A c tu a to r

Hold/Run

Select

Tab (for opening the door)

Help Menu

Figure 2-2. GP50 Display and Keypad Layout

2.1.1 Control Panel Keypad

Use the keypad to directly control pump operation, as well as to create and modify programmed series of timed events, called summary:

Press

To go from a menu to a screen, press the numeric button that corresponds to the screen’s number on the menu, or move the cursor to the desired screen name and press

to display a list of available screens.

Knob (for adjusting the contrast)

Enter

Tab (for tilting the panel)

Enter

methods

. In

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Only fields shown in reverse video on a screen can be edited. Other

fields display information only. To edit a value in a reverse video field, use the four directional arrow

buttons to move the cursor to the field. Use the numeric buttons to

2-3

GP50 Gradient Pump

enter variable values, or use the choose from among predetermined options.

Select

∆

and

Select

buttons to

∇

Keypad Buttons

To confirm the selected value, press

some screens or screen fields, pressing

NOTE A high-pitched beep sounds when you press a

button. When an error occurs, this beep is lower in frequency. The beeps can be disabled from the

MODULE SET-UP

Function

Turns the pump motor off and on. In Direct control (see Section 2.9), turnin g on the m otor cause s it to pump isocratically using the displayed eluent percentages and flow rate. In Method control (see Section 2.9.3), turning on the moto r causes it to pump at the eluent percentages and flow rate for the elapsed time of the selected method, or at the initial conditions (when the method clock is at

INIT

This button is used when priming the pump heads. pump to run at maximum vo lume (2.5 mL/min, microb ore; 10.0 mL/min, standard bore). If the pump motor is off when pump automatically turns on. To exit priming and return to the normal flow rate, press See Section B.2.8 for detailed priming in str uctions.

Prime

screen (see Section C.1.7).

again or press

or an arrow button. In

Enter

is required.

Enter

Prime

is pressed, the

Prime

Off/On

to turn off the pump motor.

causes the

2-4

Inserts a new step into a method. This button functions only when the cursor is in a

1. Move the cursor to the added below the cursor position. Parameter values in the new step are blank.

2. Fill in the time value and press

Note:

time value, the inserted step is not saved because it is incomplete.

3. Insert steps in any order. When you press automatically reorganized in the correct chronological order.

TIME

field on the

If you move the cur sor out of the

Table 2-1. GP50 Front Panel Buttons

METHOD

field and press

TIME

METHOD

or a cursor arrow button.

Enter

TIME

Enter

extension screen.

. The new step is

Insert

field before entering a

, they will be

Doc. 031377 -03 7/01

2•Description

Keypad Buttons

Function

Removes the value from the current entry field. To restore the previous value, move the cursor from the field before entering a new value.

Pressing screen “blanks” the step parameter value. Moving the cursor to another field does not restore the previo us v alue; ins tead, the step remains blank, indicating no change from the previous step.

To delete an entire method step:

1. Position the cursor in the method’s time field and press

2. Press

Turns the method clock off ( only when the pump is under Method control (see Section 2.9.3).

When the method clock is in Hold, pressing at either the initial step of a new method or, if resuming an interrupted method, at the time at which the clock was put in Hold.

When the method clock is in Run, pressing this “holds” the method and freezes the current conditions.

Delete

time is removed and the help line prompts you to press again to delete the step.

parameters, press any button except

when the cursor is in a step entry field o n the

Delete

again. Or, to restore the original time and step

Delete

) and on

Hold

(Run)

Hold/Run

METHOD

. The

Delete

. This button functions

starts the clock

stops the clock;

Doc. 031377-03 7/01

Changes the method clock time to specified by the method to occur. This button functions only when the GP50 is under Method control (see Section 2.9.3).

If the method is running, it continues running. If the method is in Hold, the method clock executes the initial conditions and holds.

When the cursor is positioned at a field with predetermined parameters, these buttons cycle through the options. In fields with predetermined numeric values, pressing

pressing

Select Enter

button increases (or decreases) the value continuously. Press

or a cursor arrow button to confirm the selected value.

Table 2-1. GP50 Front Panel Buttons (Continued)

∇ decreases the value by one unit. Holding down a

Select

, causing the initial conditions

INIT

∆

increases the value by one unit;

2-5

GP50 Gradient Pump

Keypad Buttons

Function

The four cursor buttons move the cursor, in the direction of the arrow, to the next entry field. If there is no changeable field in that direction, the cursor moves diagonally or remains in its current location.

In most cases, after entering a new value in an entry field, pressing an arrow button saves and/or executes the change. The exceptions are the

field, the

METHOD SAVE TO

command, the Displays a help screen with information pertaining to the current entry

field.

Displays one of three menus, depending on the current screen:

From an operational screen, pressing

SCREENS

From a diagnostic screen, pressing

DIAGNOSTIC MENU

From a calibration screen, pressing

CALIBRATION MENU

Enters numeric values into the current entry field. The numeric buttons are 0 through 9 and the decimal.

DIAGNOSTIC TESTS

METHOD RUN

screen, and all menu screens.

any calibration

field,

displays the

MENU of

2-6

From a menu screen, press ing a nu meri c b utt on open s t he co rres pon ding screen.

Saves and/or executes changes made in entry fields. If a menu screen is displayed, pressing

Table 2-1. GP50 Front Panel Buttons (Continued)

opens the highlighed screen.

Enter

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2.1.2 Display Screens

When the pump has successfully powered-up and passed all diagnostic tests, the the power-up, the screen. See Section C.2.7 if this occurs.

POWER-UP

screen (see Figure 2-4). If one of the diagnostic tests fails at

MAIN

DIAGNOSTIC TEST

2•Description

screen (see Figure 2-3) displays briefly, followed by

screen displays instead of the

MAIN

NOTE

Help Message

The selecting the screen from the (see Section C.2.1).

GP50 GRADIENT PUMP

Figure 2-3. Power-Up Screen

100.0 %A

0.0 %B

0.0 %C

0.0 %D

Help Message

POWER-UP

screen can be viewed at any time by

PUM P H EA D VO LUM E 100 uL

MODULEWARE REV n.nn

B IO S R E V n.n n

LOAD

2000

COLUMN A

2.00

10.27

LOCAL 2

METHOD

DIAGNOSTIC MENU

PSI

mL /MIN MIN

Doc. 031377-03 7/01

Figure 2-4. Main Screen

The allow viewing from a d istance. Use the parameters, such as the flow rate and the percentages of eluents to run.

T o access oth er GP50 screens, pre ss the

of SCREENS

screen displays status information in enlarged char acters to

MAIN

screen to select operating

MAIN

button to displ ay th e

(see Figure 2-5).

2-7

GP50 Gradient Pump

There are two ways to select a screen from a menu:

Press the numeric button on the front panel keypad that corresponds

to the screen number on the menu. For example, p re ss 3 to di splay th e

METHOD

Move the cursor to the field containing the screen number and press

Enter

See Appendix C for a description of each screen.

screen.

MENU of SCREENS

M AIN S CR E EN

DETAIL SCREEN

METHOD

DEGAS OPTIONS

Help Message

Figure 2-5. Menu of Screens

2.2 Electronics Chassis

The electronics chassis is located behind the upper door of the pump enclosure. The chassis includes se v eral ele ctronics car ds (printed ci rcuit boards) that are used to control the pump. Connectors on the cards also allow communication between the pump and other Dionex chromatography modules. Figure 2-6 shows the electronics components with the upper door open. To open the door, pull on the tab located to the right of the main power actuator (see Figure 2-2).

Do not remove any of the electronics cards from the pump. There are no user-serviceable components on the cards. If servicing is required, it must be performed by qualified personnel and appropriate electrostatic discharge (ESD) handling procedures must be followed.

MOD ULE SET-UP PUMP OPTIONS

6 7

TIME FUNCTION IN

DIAGNOSTIC MENU

2-8

Ne retirez aucune des cartes électroniques de la pompe. Auc un des composants sur les cartes ne peut être réparé par l'utilisateur. Toute réparation doit être effectuée par un personnel qualifié utilisant des procédures correctes de décharge électrostatique.

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2•Description

PWR SPY

L C

L E A K

GP50/IP25-DSP130W

DIST

MOTOR

SLOT 2SLOT 1

BLANK

L C

C O M M

P I N

D I S T

2 P

I N

SLOT 3 BLANK

SLOT 4

LAN-000K

RLY-1 OUT

RLY-2 OUT

TTL-1

OUT

TTL-2

OUT

TTL-1

TTL-2

TTL-3

TTL-4

POWER SUPPLY GREEN - OK RED - FAULT

SLOT 5

CPU/RLY

F R O N T

P A N E L

Figure 2-6. GP50 Electronics Chassis

(Located behind pump door)

LC LEAK

The leak control cable from the LC10 Chromatography Organizer or LC20 Chromatography Enclosure, connects t o the

LC LEAK

connector in

slot 1. When a leak occurs i n the LC10 or LC2 0 it is re ported to t he GP50.

Doc. 031377-03 7/01

NOTE The LC25 Chromatography Oven and LC30

Chromatography Oven do not connect to the GP50

LEAK

connector. These ovens contain internal leak

control electronics.

LC COMM

The LC30 Chromatography Oven's RJ-11 serial cable connects to the

connector in slot 1. When connected, the LC30 can be remotely

COMM

controlled by the PeakNet workstation.

2-9

GP50 Gradient Pump

LC AIR

The cable from the ai r soleno id v alv es in t he LC10, LC20, L C25, or LC3 0 chromatography module conne cts to the connected, the GP50 can electrically actuate the solenoid valves that control the position of the injection valve and the optional column switching valve in the chromatography module.

LC AIR

connector in slot 1. When

To select the valve positions, go to either the Section C.1.2) or the

TTL/RELAY

METHOD

screen (see Section C.1.4).

MAIN

screen (see

A strip of eight relay and TTL connectors is located in slot 4. These connectors interface with Dionex and non-Dionex modules for relay and TTL control of the pump. Appendix D describes the relay and TTL functions and the connections between the GP50 and other modules.

CPU

Control Moduleware for the pump resides on the CPU/Relay cards. The CPU logic and Relay I/O cards occupy slot 5. The Relay I/O card

rides piggybac k o n t he CPU card and exten ds over the f ro nt of slot 4. The card is short enough to allow the optional DX-LAN pump interface card (P/N 044195) to mount behi nd it in slot 4. A 60- pin ri bbon cab le lin ks the CPU logic to the display and keypad. The logic monitors the internal power supply outputs, and reports the status on the multicolored LED at the bottom of slot 4.

Green indicates normal operation.

Red indicates a power fault. The GP50 will enter its diagnostic state

and inhibit all other cont rols unti l the f ault is corr ected . If this occurs, turn off the power for a few seconds and then turn it back on.

2-10

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2.3 Mechanical Chassis

The mechanical chassis is housed in a pull-out drawer located behind the lower door of the pump enclosure. The front of the chassis contains the components described in Section2.4. Other mechanical assemblies are located inside the chassis drawer. The drawer should be pulled out only for service procedures. For routine operation, push in the drawer and tighten the lock located on the lower right corner of the chassis.

Observe the warning label on the inside of the lower door. The arrows on the label indicate moving mechanical parts that present pinch hazards when the pump is on and the mechanical drawer is open. Do not operate the pump with the mechanical chassis drawer pulled out.

Respectez l'étiquette d'avertissement apposée à l'intérieur de la porte inférieure. Les flèches sur l'étiquette indiquent des pièces mécaniques mobiles qui posent un danger de pincement lorsque le GP50 est sous tension et le tiroir mécanique est ouvert. N'ut ilisez jamais le GP50 avec le tiroir du châssis mécanique ouvert.

2•Description

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2-11

GP50 Gradient Pump

2.4 Mechanical Components

Figure 2-7 shows the mechanical componen ts located behi nd the lo wer door of the enclosure.

Pressure

Transducer

Pressure

Transducer

Waste Valve

Pr im ing

Block

Pump Heads

2-12

Eluent

Manifold

Proportioning

Figure 2-7. GP50 Mechanical Components

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2.4.1 Pump Eluent Manifold

Eluent lines A through D are routed through the rear of the GP50 to the vacuum de g as pump ( if ins ta ll ed) (see Sec ti on 2.5) and then to the rear of the proportioning valve. If the vacuum degas pump is not installed, the eluent lines are connected directly to the proportioning valve. The proper proportion of eluent exits the front of the valve and is directed to the priming block. Figure 2-8 shows the eluent flow path through the system.

2•Description

Eluent Reservoirs

VAC Chamber IN

VAC Chamber OUT

ABCD

Pump H eads

OUT

Proportioning Valve/

Manifold Assembly

Pressure Transducer

Figure 2-8. Eluent Flow Schematic

Mixer To Column

Rinse Ports

Priming Block

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2-13

GP50 Gradient Pump

2.4.2 Pump Heads

There are two types of pump heads: standard bore and microbore. The table below summarizes the features and operating conditions for each type.

Pump Head Type

Standard Bore

Microbore 25µL Isocratic and

*Flow rates are adjustable in increments of 0.01 mL/min.

NOTE Although there is some overlap in flow rates

Piston Volume

100µL Isocratic 0.04–10.0 35 MPa

between the two pump configurations, continuous operation of the microbore pump at flow rates above 2.0 mL/min will decrease seal and pump life. For the best extended operation at 2.0 mL/min or above, use a standard bore pump. To achieve optimum performance at flow rates below

0.4 mL/min, use a microbore pump.

Pump Operation

Gradient 0.4–10.0 35 MPa

Gradient

Flow Rate (mL/min)*

0.0–2.50 35 MPa

Maximum Operating Pressure

(5000 psi)

2.4.3 Pump Mixers

A pump mixer ensures complet e mixi ng of the proportioned eluents prior to injection. The mixer is installed between the pump outlet and the injection valve. Three types of mixers are available:

2-14

In standard bore PEEK pumps, a GM-5 Mixer (P/N 054044) is used. In microbore PEEK pumps, a GM-4 Mixer (P/N 049136) is used. In stainless ste el pumps , a Sta inles s Ste el Mi x er (P /N 0 54043) is use d.

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2.4.4 Pump Priming Block

The priming block “tee” directs the flow of eluent fr om the manifo ld i nto the pump heads. The priming block is also used for rapid removal of air from the system.

Refer to Section B. 2.8 for instructions on priming the pump heads.

2.4.5 Pressure Transducer

From the priming block, the liquid stream is directed to the inlet check valves on the pump heads, through the pump heads, and finally through the outlet check valves to the pressure transducer.

Flow from the outlet check valves on the pump heads is combined in the pressure transducer. The pressure transducer measures the system pressure at this point. The interactive constant-flow/constant-p ressure control program on the DSP precisely controls the pump motor speed to assure flow rate accuracy.

A waste line exits the bottom of the pressure transducer. Opening the valve on the pressure transducer diverts flow to the waste line and relieves system pressure, forcing air out of the system.

2•Description

Doc. 031377-03 7/01

Flow output from the pressure transducer is directed out of the pump module, through the gradient mixer, and on to the rest of the chromato graphy system (inj ection valve, column, detector).

See Section B.2.6 for eluent outlet line connections.

2-15

GP50 Gradient Pump

2.5 Vacuum Degas Pump Assembly (Optional)

The Dionex vacuum degas pump provides continuous on-line vacuum degassing of up to four eluents. The assembly, which must be installed at the factory, consists of :

A 4-channel degas chamber (with degas membranes) with 17 mL internal

capacity p er channel A dual-stage diaphragm vacuum pump

A solenoid valve

An on-board vacuum sensor

The electronics required to operate the vacuum pump

Fittings, tubing, and other accessories

By default, the de gas pump tur ns on f or 2 minu tes when th e GP50 power is turned on. Thereafter, the pump turns on for 30 seconds at 10-minute intervals. The

DEGAS OPTIONS

Section C.1.6). You can check the vacuum chamber pressure from the

STATUS

screen (see Section C.3.4).

screen allows you to change the cycle time and duration (see

DEGAS

NOTE All components of the vacuum degas assembly are made

of inert materials or corrosion-resistant materials. However, Dionex recommends thoroughly flushing any chemicals out of the tubing with deionized water after each use to avoid crystallization in the membrane pores.

2.6 Piston Seal Wash (Optional)

When using highly concentrated buffer solutions, Dionex recommends continuously rinsing the piston seal with a piston seal wash. Rinsing removes salt crystals that may abrade the piston, thereby causing the seal to wear out prematurely and allow leaks.

Dionex offers a continuous seal wash kit (P/N 059187) for the GP50. For installation instructions, see Section B.2.8 or the instructions included with the kit.

2-16

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2.7 Eluent Reservoirs

NOTE Dionex strongly recommends degassing all eluents and

storing them in reservoirs pressurized with helium. This helps prevent bubbles (resulting from eluent outgassing) from forming in the el uent proportionin g valves, pump heads, and the detector cell.

Degassed eluents and pressurized reservoirs are especially important when combining aqueous and nonaqueous components (e.g., water and methanol). Pressurizable reservoirs allow eluents to be stored under a specific atmosphere.

The following pressurizable reservoirs are available from Dionex:

1-liter glass reservoirs with shatterproof plastic coating (P/N 044126)

2-liter glass reservoirs with shatterproof plastic coating (P/N 044127)

1-liter plastic reservoirs (P/N 044128)

2•Description

2-liter plastic reservoirs (P/N 044129)

Do not use the 2-liter plastic reser voir (P/N 044129) for off-line vacuum degassing of eluents. The reservoir was not designed for this purpose.

N'utilisez pas le réservoir en plastique de 2 litres (N /P 044129) pour le dégazage à vide hors ligne d'éluants. Le réservoir n'a pas été conçu à cette fin.

Refer to the

Pressurizable Reservoir Installation Instructions

(Document No.

034581) for installation details.

EO1 Eluent Organizer (Optional)

The Dionex EO1 Eluent Organizer (P/ N 044 125) holds eluent reservoirs in a liner that contains spills and leaks. The EO1 can also be used to pressurize reservoirs. Up to two optional E01 Eluent Organizers can be places on top of the system

Doc. 031377-03 7/01

2-17

GP50 Gradient Pump

enclosure. Each organizer can accommodate one or two reservoirs, depending on the type of reservoir used (see the table below).

No. of Reservoirs

2 1-Liter glass or plastic 2 L 2 2-Liter plastic 4 L 1 2-Liter glass 2 L

Description Total Volume

2.8 Rear Panel

The rear panel contains the main power receptacle and fuses. It also includes a DX-LAN connector for interfacing the GP50 with the PeakNet workstation. The rear panel is illustrated in Figure B-1.

2.9 Functional Description

2.9.1 Operating and Control Modes

The operating mode determines commands:

In Local mode, the GP50 receives commands from the front control panel buttons and screens.

in Liters

how

the GP50 receives operating

2-18

In Remote mode, PeakNet 5 software sends commands from the host

computer via the DX-LAN int erfac e. Limited o perating changes fr om the front panel are allowed.

In Locked Remote mode, PeakNet 5 or PeakNet 6 software sends commands from the host computer via the DX-LAN interface. All operating changes from the f ront pane l are disab led. When PeakNet 6 software is controlling the GP50, the pump is always in Locked Remote mode.

The control mode determines

In Direct control, the GP50 exe cutes commands immediately.

when

operating commands are executed.

Because there is no time-ba sed program, th e method clock i s not used and the

Hold/Run

and

buttons do not operate.

Reset

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2•Description

In Method control, the GP50 executes commands according to the timed steps in a method. The method is programmed from the GP50 front panel or from PeakNet 5 software. See Section 2.9.3 for details about Method control from the front panel. using PeakNet 5 to program and run methods, refer to the onli ne Help or user’s guide.

The table below summarizes the various operating and control mode configurations. Select the modes from the Section C.1.2),

DETAIL

screen (see Section C.1.3), or chromatography

software.

Operating/Control Mode Pump Operat ion

Local/Direct Control Commands are entered from the GP50 front control

panel and executed immediately after being entered.

Local/Method Commands are entered from the GP50 front control

panel and executed by running a method programmed from the front panel.

Remote/Direct Control Commands are sent from PeakNet 5 and executed

immediately when received.

For information about

screen (see

MAIN

Locked Remote/Direct Control

Remote/Method Commands are sent from PeakNet 5 and executed by

2.9.2 Local and Remote Modes

Local Mode

When the GP50 power is turned on, the pump is in Local mode. In Local mode the pump accepts operating commands from two sources:

Direct input from the front panel keypad and screens. With direct input, all GP50 operating functions are available.

TTL inputs from a remote controller (for example, a Dionex detector module or autosampler). TTL signals can be used to turn the pump motor on and off, turn the method clock on and off, and increment or decrement the method number.

Doc. 031377-03 7/01

Commands are sent from PeakNet 5 or PeakNet 6 and executed immediately when received.

running a method programmed in PeakNet 5.

2-19

GP50 Gradient Pump

Remote Mode

In Remote mode, PeakNet 5 software sends operatin g commands fr om the host computer via the DX-LAN interface. In Remote mode, operating parameters can be changed from the front panel, provided they do not interfere with a running method.

Locked Remote Mode

In Locked Remote mode, PeakNet 5 or PeakNet 6 software sends operating commands from the host computer via the DX-LAN interface. In Locked Remote mode, all operating changes from the front panel are disabled.

When the GP50 is controlled by PeakNet 5, select the Locked Remote Start option from the PeakNet 5 Run program to operate in the Locked Remote mode. To return the GP50 to Local mode, clear the Start opt ion or turn the GP50 power off and then on.

When the GP50 is controlled by PeakNet 6, connecting to the PeakNet 6 timebase automatically selects the Locked Remote mode. To return the GP50 to Local mode, either clea r the Connect check box on the PeakNet 6 control panel or turn the GP50 power off and then on.

2.9.3 Method Control

In Method control, commands are executed according to the time-based steps programmed in a method. Each step specifies the eluent composition and flow rate to be delivered by the pump at a given time. The selected eluent mixture is delivered either isocratically, or as a multistep linear or curved gradi ent. As the method runs, the GP50 calculates the changes i n eluent compositio n require d to deli v er a gradient from one method step to the next or to match the selected curve.

2-20

Doc. 031377 -03 7/01

2•Description

Methods are programmed, saved, and edited from the

METHOD

(see Figure 2-9). See Section 3.3 for programming instructions.

M E TH OD E DIT

SAVE TO RUN 100605

LIMITs 0 - 5000

TIME % A

IN IT

0.00

123.45

345.67

25.0

100.0

10.0

17.2

25.0

0.0

22.2

19.6

25.0

0.0

32.3

33.2

25.0

0.0

35.5

30.0

C%B %C %D

L1.00

I L

FLOW

2.00

Help Message

Figure 2-9. Method Screen

NOTE For information about using PeakN et 5 to program and

run methods, refer to the online Help or user’s guide.

Here is a summary of basic information about using methods.

Each method can contain up to 50 time-based steps. Step 1 always

starts at The GP50 can store up to 100 separate methods (0 through 99) in

(initial condition). Step 2 always starts at

INIT

memory. Methods are retained in memory even after the pump is powered down.

PSI

> >

TIME = 0.0

screen

Doc. 031377-03 7/01

The total number of methods that can be stored in memory depends on the length of each method and the amount of available memory; thus, the actual total may be less than 100.

The pump can run under method control while you are entering or

editing any method. When you save changes to the currently running method or switch to

a different method, the method clock continues running unaffected. Only those parameter changes which affect the method

after

the

current time will be implemented in the current run.

2-21

GP50 Gradient Pump

2.9.4 Eluent Delivery

Isocratic Eluent Run

The simplest use of the GP50 is for the delivery of an isocratic (unchanging) mixture of one or more eluents. If more than one eluent is selected, the pump d elivers a proport ional mixt ure of the el uents based on the percentage of each eluent selected. The combined percentages of all eluents selected must total 100% or the pump will not run.

Gradient Eluent Run

The GP50 can produce step, linear, concave, or convex curves in eluent concentration over a specified time period. The slope of the gradient is determined by the selected gradient curve (see Figure 2-10) and the time between the starting and end ing points of a grad ient step. It is important to note the following points:

The curve number p arameter determines whethe r the pump deliver s a

linear or curved gradient.

2-22

1.0 .9

.7 .6 .5

.3 .2 .1 0

45678

910

Figure 2-10. Eluent Composition Profile for Curves 1 - 9

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2•Description

Curve numbers are defined as follows:

Curve Number Gradient Type

1, 2, 3, 4 Convex 5 Linear (power-up default) 6, 7, 8, 9 Concave

NOTE A curve number in a step instructs the pump to use

the selected curve number when moving from the previous step to that step. Because there are no previous steps for cannot be entered for these steps.

The gradient slope does not change during a step if curve 5 (the

INIT

default setting) is selected, because curve 5 represents a linear gradient.

Convex curves cause rapid changes in eluent composition at the

beginning of the curve and slower changes at the end. Concave curves cause slower changes at the beginning and rapid changes at the end.

TIME=0.0

, curve numbers

Slope changes o ver time become mor e ex treme as c urves go from 6 t o

9 (more concave) and from 4 to 1 (more convex). Figure 2-10 shows the eluent composition profiles corresponding to curves 1 through 9, normalized for 0–100% for 10 minutes.

Any consecutive method steps specifying identical eluent

compositions will generate an isocratic segment, regardless of the curve number selecte d.

A step change is a steep linear gradient in which the eluent

composition changes from one eluent to another within 6 seconds.

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2-23

GP50 Gradient Pump

2-24

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3 • Operation and Maintenance

3.1 Getting Ready to Run

NOTE The GP50 Gradient Pump is designed to perform ion

chromatography and BioLC applications only and should not be used for any other purpose. If there is a question regarding appropriate usage, contact Dionex.

3.1.1 Degas Eluents

Dionex strongly recommends degassing all eluents and storing them in reservoirs pressurized with filtered inert gas (see Sect ion 3.1. 3). This helps prevent bubbles caused by eluent outgassing from forming in the eluent proportioning valves, pump heads, and the detector cell. Degassed eluents and pressurized reservoirs are especially important when combining aqueous and nonaqueous components (e.g., water and acetonitrile).

The GP50 with the optional vacuum degas pump assembly provides continuous on-line vacuum degassing of up to four eluents.

Doc. 031377-03 7/01

If the GP50 is not equipped with the vacuum degas assembly, manually vacuum-degas eluents daily, as described below, and store them in pressurized reservoirs.

Degassing Eluents Manually

1. Prepare the eluent required for your applicat ion. Pour it into a v acuu m flask and attach the flask to a vacuum pump or water aspirator.

2. Vacuum degas the eluent for 5-10 minutes by shaking or sonication.

3. Remove the flask from the vacuum.

the aspirator back into the flask.

4. Pour the degassed eluent into a press uriza ble res ervoir . Be car eful not to shake the e luent.

5. Install end-line filters and press urize the re servoi rs (see Sectio ns 3.1.2 and 3.1.3).

Do not allow water to flo w fr om

3-1

GP50 Gradient Pump

3.1.2 Filter Eluents

Always f il t er e lue nt s bef or e beginning operation. Fil ter in g re moves small particulates that may contaminate the eluent proportioning valves or the pump check valves and cause erratic flow rates or loss of prime. The pressurizable reservoir Ship Kits supply end-line filters (P/N 045987) for this purpose.

Install an end-li ne f il ter on the end of each elu ent li ne insi de the re se rv oir. To prevent air from being drawn through the lines, mak e sure that the end of each filter r eaches the bot tom of the el uent reser voi r and that eac h fi lter is submerged in eluent.

3.1.3 Pressurize Eluent Reservoirs

The GP50 Gradient Pump is capable of operation with or without head pressure on the eluent. Pressurization of the eluent reservoirs, if used, should be with filtered inert gas (preferably helium). Refer to the

Pressurizable Reservoir Installation Instructions

1. V erify that a re gu lator (P/ N 046594) is install ed on the g as supp ly line to the reservoirs.

for details.

3-2

2. Turn on the gas supply and adjust the pressure to 55 KPa (8 psi).

Never pressurize the reservoirs above 69 KPa (10 psi).

Ne mettez jamais les réservoirs d'éluants sous une pression supérieure à 69 kPa (10 lb/po²) .

Doc. 031377 -03 7/01

3.1.4 Start-Up

1. Turn on the main pump switch.

3 • Operation and Maintenance

The

POWER-UP

screen displays (see Figure 2-4). A series of diagnostics tests is

MAIN

run at power -up. If one of the test s fail s, the

screen displays briefly (see Figure 2-3) and then the

DIAGNOSTIC TEST

displays instead. See Section C .2.7 if this occurs. When the GP50 powe r is turned on, the injection v alv e is initia lized to

the Load position.

2. Press

Off/On

3. Check the pressure reading on the

to start the pump flow.

screen. The GP50 display

MAIN

updates the pressure readout once pe r piston str oke. The rea ding from one stroke to the next should be within 3%.

A variation of more than 3% indicate s that the pump is out of prime. Refer to Section B.2.8 for priming instructions, or see Section 4.1 for other conditions which can cause the pump to lose prime.

NOTE After starting the pump or changing the flow

rate, wait at least 5 minutes (up to 20 minutes for low flow rates in a standard bore pump) before beginning an analysis. This allows the pump's real-time electronic pulse damping circuitry to stabilize the flow rate.

screen

Doc. 031377-03 7/01

3-3

GP50 Gradient Pump

3.1.5 Selecting the Pressure Limits

The high and low pres sur e li mit s aut omat ic all y st op the pump if a sy stem malfunction occurs, such as overpressurization caused by a blockage or low pressure caused by a leak downstream from the pump.

When PeakNet is controlling the pump, select the pressure limits

from the software. When the pump is running under Local mode, Direct control, enter

the pressur e limits on the When the pump is running under Local mode, Method control, enter

the pressure limits on the are set in the When a limit trip stops t he pump, the method c lock immediate ly stops and goes to Hold. The current status of the program that was running at the time is displayed on the front panel.

To select the pressure limits from the front panel:

step and remain unch anged t hro ughout the ana lysis .

INIT

DETAIL

METHOD

screen (see Figure 3-1).

screen (see Figure 3-5). The limits

1. Go to the

DETAIL

METHOD

screen and move the cursor to the

field.

2. Enter a low pressure limit between 1.4 and 2.8 MPa (200-400 psi).

DETAIL SCREEN

100.0 %A

0.0 %B

0.0 %C

0.0 %D

LOCAL

Help Message

Figure 3-1. Detail Screen: Setting Pressure Limits

2125

2.00

IN J E C T

COLUMN

LIMIT

DIRECT CNTRL

PSI m L/M IN SAMPLE A

300-2500 PSI

TTL1 TTL2 RLY1 RLY200

1 1

The setting may vary, depending on the system operating pressure. The low pressure limit is activated after 13 pump piston strokes (i.e., after 1.3 mL (standard bore) or 0.325 mL (microbore) of fluid is pumped through).

3. Enter a high pressure limit that is 2.8 to 3.4 MPa (400-50 0 psi ) above the normal system operating pressure. The pump is equipped with a pressure limit that prevents operation above 35 MPa (5076 psi).

LIMIT

3-4

Doc. 031377 -03 7/01

3 • Operation and Maintenance

3.1.6 Selecting the Operating and Control Modes

1. Go to either the displays displays

2. To select the operating m ode:

3. To select the control mode, move the cursor to the field, press

Select

a cursor arrow button.

LOCAL, REMOTE DIRECT CNTRL

GP50 is controlled by PeakNet 6

If the

PeakNet 6 timebase a utomatica lly select s the GP50 to PeakNet 6 control panel or turn the GP50 power off and then on.

GP50 is controlled by PeakNet 5

If the

on the

Select Enter

To select Remote Start option from t he Run program. To return the GP50 to

LOCAL

MAIN

∆

or a cursor arrow button.

LOCKED RMT

, clear the Start op tion o r turn the GP50 po wer of f and then

Select

∆

MAIN

LOCAL

Select

DETAIL

, either clear the Connect check box on the

DETAIL

to toggle to the desired mode, and press

∇

to toggle to the desired mode, and press

∇

screen. The operating mode field

, or

LOCKED RMT

METHOD

screen. Move the cursor to the field, press

with PeakNet 5, select the Locked

. See the example in Figure 3-2.

. The control mode field

, connecting to the

LOCKED RMT

select

LOCAL

. To return

REMOTE

Enter

25.0 %B

15.0 %C 0 .0 %D

Doc. 031377-03 7/01

60.0 % A

LOAD

COLUMN A

LOCA L

2000

2.00

DIRECT CNTRL

PSI

m L/MIN

Help Message

Control ModeOperating Mode

Figure 3-2. Main Screen: Operating and Control Mode Fields

3-5

GP50 Gradient Pump

3.2 Running Under Direct Control (Local Mode)

Direct Control, Local mode is use d most often when a PeakNet workst atio n is not configured.

Direct Control Example

Specify an isocrati c mi xture of 60% eluent A, 25% eluent B, and 15% eluent C to be pumped at 2.0 mL/min (see Figure 3-3).

3-6

1. Go to the control modes to

Figure 3-4 illustrates the up.

MAIN

60.0 % A

25.0 %B

15.0 %C 0 .0 %D

Help Message

Figure 3-4. Main Screen: Running Under Direct Control

Figure 3-3. Isocratic Run Profile

LOCAL

DETAIL

screen and if necessary, change the operating and

and

DIRECT CNTRL

screen as it wil l appear when the e xa mple is set

MAIN

LOAD

2000

COLUMN A

LOCA L

DIRECT CNTRL

(see Section 3.1.6).

PSI

2.00

m L/MIN

Doc. 031377 -03 7/01

3 • Operation and Maintenance

2. Move to the %A field and enter 60; move to the %B field and enter 25; move to the eluent percentages equa l 100).

field and press

(15 is automatically filled in to ma ke the

Enter

3. Move to the

mL/MIN

4. If the pump is currently off, press

field and enter 2.

Off/On

to turn on the motor and begin the

isocratic delivery.

NOTE After starting the pump or changing the flow rate, wait

at least 5 minutes (up to 20 minutes for low flow rates in a standard bore pump) before beginning an analysis. This allows the flow rate to stabilize.

3.3 Running Under Method Control (Local Mode)

This section pro vi des general instructions on how to create, edit , and run methods from the GP50 front panel. For step-by-step examples, see Section 3.4.

When entering parameters on the

After starting the pump or changing the flow rate, wait at least 5 minutes (up

METHOD

to 20 minutes for low flow r ates in a sta ndard bore p ump) befor e beginning an analysis. T his allows the flow rate to stabilize.

In the %A, %B, %C, and %D columns, enter decimal percentage values from

0.1% through 100% for the eluent compositions. The combined percentages for all eluents must total 100% or the pump will not run.

In the V column, select the injection valve posi tion (L for load or I for inject).

screen, observe these guidelines:

In the

increments of 0.01 mL/min. See Section 2.4.2 for the available flow rate ranges. They vary, depending on the size of the pump head and whether the run is isocratic or gradient.

For steps other than

The curve num ber determines whether the pump delivers a lin ear or curved

Doc. 031377-03 7/01

column, ent er the pump flow rate. Fl ow rates are adjustable in

FLOW

Continuous operation of the microbore pump at flow rates above 2.0 mL/min will decrease seal and pump life. For optimum performance above 2.0 mL/min, a standard bore pump should be used.

INIT

and

TIME=0.0

, enter a curv e number in the C column.

3-7

GP50 Gradient Pump

gradient when moving to the step from the one preceding it (see Section 2.9.4). The default is curve 5 (linear).

NOTE Because there are no previous steps f or

curve numbers are not entered for them.

If a step field is “blank” (has no entry), the last selected value for the field

INIT

TIME=0.0

remains in effect. The symbol

next to the bottom time ent ry indicates there are addit ional steps

∨

below. Move the cursor to the bottom time entry and press the down arrow to see the additional step(s).

The symbol ^ next to the top time entry indicates that it is preceded by at least

one more step. Move the cursor to the entry and press the up arrow to see the additional step(s).

The symbol > at the right edge of each line indicates a lateral extension to the

line. Move the cursor to the end of a line and press the right arrow to display the

METHOD

extension screen (see Section C.1.5).

3.3.1 Creating a New Method

You can create a new method when the method clock is in either

Run

1. Go to the

MAIN

DETAIL

operating and control modes to Section 3.1.6).

screen and if necessary, change the

LOCAL

and

METHOD

(see

Hold

3-8

2. Go to the

3. In the

EDIT

METHOD

field, enter the number of the method to be created. This

screen.

can be the number of an unused method or of an existi ng met hod that you plan to edit and sa v e as a new method. If you enter the number of an unused method, the screen will look similar to th e example screen in Figure 3-5.

Doc. 031377 -03 7/01

3 • Operation and Maintenance

M ETHO D E DIT

TIME %A

100.0

0.00

Help Message

Figure 3-5. Method Screen: Creating a New Method

4. In the

%B %C %D V

field, set the low and high pressure limi ts (see

LIMITs

SAVE TO RUN

LIMITs

0 - 5000

C FLOW

L1.00IN IT

PSI

> >

Section 3.1.5).

5. Each method starts out with two timed steps (see Figure 3-5): an initial conditions st ep (contai ning zero step (containing 0.00 in the

in the

INIT

column). The parameters in

TIME

column) and a time

TIME

each of these first two steps can be changed but the steps cannot be deleted. Enter the parameters for both steps.

6. Enter a new step using one of the following methods:

Move the cursor to the empty

field below the last step and

TIME

enter the elapsed time at whic h to start the new step. Press or a cursor arrow button.

Enter

Doc. 031377-03 7/01

Move the cursor to any of the

fields and press

TIME

Insert

. This adds a ne w step af ter th e curs or posit ion. Ente r the elapsed time at which to start the new step and pres s

or a cursor arrow

Enter

button.

After entering a new step, all timed steps are automatically organized in chronological order.

7. Continue entering parameters for the new step. When you finish, move the cursor to the

If you are editing an exist in g metho d, ent er a new number for the

method and press If you are editing an unused method, press

SAVE TO

Enter

field and do one of the following:

Enter

3-9

GP50 Gradient Pump

3.3.2 Running a Method

1. If the pump motor is off, press

2. Go to the

MAIN

DETAIL

screen and if necessary change the

operating and control modes to

to turn on the motor.

Off/On

LOCAL

and

METHOD

(see

Section 3.1.6).

3. In the

METHOD

# field, enter the desired method number. If the method clock is already runn ing, the method starts immediatel y . If the clock is in Hold, press

NOTE You can also select the method number in the

METHOD

and enter the desired method number.

Hold/Run

screen. Move the cursor to the

to start the method.

RUN

fiel d

4. The elapsed time on the method clock when the method begins determines where (at what step and parameters) the method begins running:

If the method clock is at

running using the If the method clock is greater than zero, the method begins

ial condition parameters.

INIT

or time zero, the method begins

INIT

running using the par ameters s pec if ie d in the s tep f or t hat e lap sed time. To start the method at the

Reset

ial conditions instead, press

INIT

3-10

Doc. 031377 -03 7/01

3.3.3 Editing a Method

Existing methods can be modified by changing, adding, or deleting steps and parameters. Changes can be made when the method clock is stopped or running. If the meth od you ar e editin g is curre ntly runn ing, the cha nges are stored in memory and implemented when you save the method.

NOTE After saving changes, there is no way to recall the

original method. If you plan to make experimental changes to a method but also want to retain the original method, save the modified method to a new number.

To edit a method:

3 • Operation and Maintenance

1. Go to the

METHOD

screen. In the

field, enter the number of the

EDIT

method to be modified.

2. Make the required changes:

To change a parameter

, position the cursor in the field and enter

the new value. The previo us value is automatically deleted.

To add a method step

, or move the cursor to the empty

Insert

, move the cur sor to an y

TIME

field and press

TIME

field below the last step and enter the elapsed time at which to start the new step. When you press

or a cursor arrow button, the new step is

Enter

automatically moved to the correct chronological position. Continue entering parameters for the new step.

To delete a method step

and press

Delete

3. When changes are complete, move the cursor to the Press

to save the changes to the current method, or enter a new

Enter

method number and press

, move the cur so r to th e step to be deleted

twice.

SAVE TO

Enter.

If you save changes to the currently running method, they are immediately incorporated in the run and execut ed at the prog rammed time, the method at the method, press

the modified event has already been executed. To restart

unless

ial conditions and run all steps of the updated

INIT

Reset

field.

Doc. 031377-03 7/01

3-11

GP50 Gradient Pump

3.3.4 Deleting a Method

To delete an entire me th od, move the cursor on the

step and press

INIT

Delete

twice.

3.3.5 Changing the Running Method

To change from t he method currently running t o a di fferent method, g o to the field, and press

MAIN

DETAIL

Enter

screen, enter the new method number in the

The new met hod will begin runnin g, u si ng the parameters speci fied in the step for the current elapsed time. To start the method at the conditions, press

Reset

3.3.6 Controlling the Method Clock

The

Hold/Run

and

DETAIL

To start and stop the method clock, press

To reset the clock to

To set the clock to a specific elapsed time, enter the time in the

field on the continue) running, using the method parameters specified for that time.

button, the

button, and the

Reset

screens control the method clock:

ial conditions, press

INIT

MAIN

DETAIL

screen. The method will start (or

METHOD

field s in the

MIN

Hold/Run

Reset

screen to the

INIT

METHOD

ial

MAIN

MIN

3-12

Doc. 031377 -03 7/01

3.4 Example Methods

The examples in this section provide step-by-step instructions for creating three types of methods: iso crati c, linea r gra dient, and cur v ed g radie nt. The l ast ex ample demonstrates how to edit a running method.

3 • Operation and Maintenance

For all of the examples, set the pump to

LOCAL

Section 3.1.6).

3.4.1 Isocratic Method Example

Specify an isocratic mixture of 60% eluent A, 25% eluent B, and 15% eluent C to be pumped at 2.0 mL/min. Figure 3-3 illu strates the isocratic profile for this example. Figure 3-6 illus trates the appears when the example is set up .

M ETHO D E DIT

TIME %A

60 .0_ 25.0 15.0

0.00

Help Message

Figure 3-6. Method Screen: Isocratic Run Example

1. Go to the

%B %C %D V

METHOD

screen and enter a method number in the field (1, for example). The screen auto matically changes the number in the

SAVE TO

field to the number of the method being edited.

SAVE TO

LIMITs 0 - 5000

mode,

METHOD

C FLOW

METHOD

RUN

L2.00IN IT

control (see

screen as it

011

PSI

> >

EDIT

Doc. 031377-03 7/01

If Method 1 currently e xis ts and you w ant to ret ain it, e nter a ne w,

unused, method number in the If Method 1 currently exists and you want to delete it, move the

cursor to

TIME=INIT

2. Move the cursor to the and enter 25; move to the

and press

field and enter 60; move to the %B field

field and press

EDIT

Delete

field.

twice.

Enter

(15 is automatically filled in to make the eluent percentages equal 100). Ignore the

(Curve) an d V (Valve) fields. Mov e to the

FLOW

enter a flow rate of 2.00.

field and

3-13

GP50 Gradient Pump

3. Move the cursor to

4. Move the cursor to press off, press

to select the programmed method. If the pump motor is

Enter

Off/On

SAVE TO

Run

to have the pump st ar t del ivering the eluen t mixt ur e.

5. If the method clock is in hold, press

and press

, enter the method number (1, in this case) and

Hold/Run

method.

3.4.2 Linear Gradient Method Example

The following summarizes the linear gradient method steps:

Create Method 2 to be gin under i socratic co nditions with 10 0% eluent

A at 2.0 mL/min. After 5 minutes, begin adding eluent B and decreasi ng eluen t A until,

at 10 minutes, the mixture is 65% eluent A and 35% eluent B. Begin adding eluent C to the mixture while continuing to decrease

eluent A and increase eluent B until, at 15 minutes, the eluent composition is 0% eluent A, 50% eluent B, and 50% eluent C.

Continue increasing eluent C and begin decreasing eluent B until, at

25 minutes, the eluent composition is 100% eluent C. Make a step change to 100% eluent D at 25.01 minutes. Pump 100%

eluent D for 4.99 minutes.

to save the method.

Enter

to begin running the

3-14

At 30.01 minutes, return to 100% eluent A and re-equilibrate your

system for the next analysis.

Figure 3-7 illustrates the gradient profile for this method.

Doc. 031377 -03 7/01

3 • Operation and Maintenance

Figure 3-7. Linear Gradient Method Profile

1. Go to the

METHOD

screen and enter a method number in the

field (2, for example).

If Method 2 currently e xis ts and you w ant to ret ain it, e nter a ne w,

unused, method number in the If Method 2 currently exists and you want to delete it, move the

cursor to

2. Move to the

TIME = INIT

FLOW

Figure 3-8 illustrates the

and press

field of the

METHOD

INIT

field.

EDIT

Delete

twice.

step and enter a flow rate of 2.00.

screen as it app ear s so far. You can now begin entering the method steps that will generate the gradient profile.

M ETHO D E DIT

SAVE TO RUN 0

LIMITs 0 - 5000

TIME %A

100.0

%B %C %D V

C FLOW

L2.00IN IT

0.00

Help Message

Figure 3-8. Linear Gradient Method Example (After Step 2)

PSI

> >

EDIT

Doc. 031377-03 7/01

3-15

GP50 Gradient Pump

3. Move the cursor to the %A field of the

TIME = 0

step and press

100% of eluent A is automatically filled in.

4. Move the cursor to the line below at

TIME = 5.0

minutes. Then mo v e the cu rsor to %A and press

TIME = 0,

and enter 5 to store a step

enter 100% and defi ne a step with the same elu ent compos ition as the previous step. Although there is no change in eluent parameters, the 100% of %A must be entered at 5.00 minutes to establish 5.00 as the gradient start point.

This marks the end of the isocratic section of the run and the beginning of t he el uent B concentration ramp. From this point on, t he concentration of eluent A decreases from 100% as the concentration of eluent B begins to increase from 0%.

5. Move the cursor to the next line. Ente r 10 in the cursor to the

field and enter 65, followed by 35 in the %B field.

field . Move the

TIME

After a total of 10 minutes (5 minutes of isocratic conditions plus 5 minutes to gradually decrease the amount of eluent A in the mixture while increasing the amount of eluent B), the eluent composition is 65% eluent A and 35% eluent B.

This step marks the end of the second segment and the beginning of the eluent C concentration ramp. Figure 3-9 illustrates the

METHOD

screen as it appears after Step 5.

Enter

3-16

M ETHO D E DIT

SAVE TO RUN 0

LIMITs 0 - 5000

TIME %A

100.0

0.00

5.00

10.00

100.0

65.0 35.0

%B %C %D V

C FLOW

L2.00IN IT

H e lp Mes s a ge

Figure 3-9. Linear Gradient Method Example (After Step 5)

PSI

> >

Doc. 031377 -03 7/01

3 • Operation and Maintenance

6. Move the cursor to the next line. Ente r 15 in the cursor to the

field and enter 50, followed by 50 in the %C field.

field. Move the

TIME

Beginning with the method step immediately preceding this one (

TIME=10

), the pump begins adding el uent C, starting with 0%. When the method reaches this step (after 15 minutes), eluent C is at 50%, eluent B at 50%, and eluent A at 0%.

7. Move the cursor to the next line. Ente r 25 in the cursor to

and enter 100.

field. Move the

TIME

After a total of 25 minutes, the concentration of e luent B drops to 0% and the concentration of eluent C increases to 100%.

8. Move the cursor to the next line. Enter 25.01 in the the cursor to

and enter 100.

field. Move

TIME

The concentration of eluent C drops to zero and the concentration of eluent D, which the pump began adding 0.6 seconds (0.01 min) earlier, reaches 100%. This is a

step change

in eluent composition to 100% eluent D. A step change is a v ery st eep lin ear gra dient in which the eluent composition changes from one eluent to another in 6 seconds. Figure 3-10 illustrates the

METHOD

screen as it appears

after Step 8.

M ETHO D E DIT

Doc. 031377-03 7/01

SAVE TO RUN 022

TIME %A

10.00

65.0 35.0

15.00

25.00

25.01

LIMITs 0 - 5000

%B %C %D V

C FLOW

50 .0 50 .0

100.0

PSI

2.00

Help Message

Figure 3-10. Linear Gradient Method Example (After Step 8)

9. Move the cursor to the next line. Ente r 30 in the cursor to the

field and ente r 100. The el uent co mpositi on remai ns

field. Move the

TIME

unchanged at 100% eluent D for 4.99 minutes.

10. Move the cursor to the next line and enter 30.01 in the Move the cursor to the

field and enter 100. This causes another

step gradient from 100% D to 100% A.

> >

TIME

field .

3-17

GP50 Gradient Pump

11. Move the cursor to the

SAVE TO

field and press

method to memory. Figure 3-11 illustrates the completed method.

M ETHO D E DIT

SAVE TO RU N 022

LIMITs 0 - 5000

TIME %A

25.00

25.01

30.00

30.01

100.0

%B %C %D V

100.0

C FLOW

Help Message

Figure 3-11. Linear Gradient Method Example (Complete)

12. Press

METHOD

and

field. Press

Enter

Reset

to go to the

screen. Enter 2 in the

MAIN

to reset the method to the

necessary).

13. If the pump motor is off, press

14. If the method clock is in hold, press

to start the pump.

Off/On

Hold/Run

to start the method running. When the method reaches the last step in the method (

TIME = 30.01

), the pump will continue to pump isocratically until the

clock is reset.

Enter

2.00

to save the

PSI

> >

step (if

INIT

3-18

Doc. 031377 -03 7/01

3 • Operation and Maintenance

3.4.3 Curved Gradient Method Example

When attempting to optimize the separation of a single component in a complex mixture, it is often helpful to employ paired segments of a curved gradient. For example, if the analyte is an oligonucleotide that elutes with 0.32 M NaCl in a linear gradient run, you can move potentially interfering components of the sample away from the target oligonucleotide b y fir st programming a se gment from 0% to 32% of a 1 M NaCl solution using curve 2. Then, program a segment from 32% to 100% of 1 M NaCl using curve 8. The resulting gradient profile is shown in Figure 3-12.

Doc. 031377-03 7/01

Figure 3-12. Gradient Curve Profile

In binary curve gradients, two elements follow curves which are mirror images (e.g., E1 = Curve 8 and E2 = Curve 1 in Figure 3-13). See Section 2.9.4 for details about gradient curves.

3-19

GP50 Gradient Pump

The following summarizes the curved gradient method example steps:

Create Method 3 to be gin under i socratic co nditions with 10 0% eluent

A (25 mM Tris buffer, pH 8.0) at 1.5 mL/min. After 1 minute, begin a convex addition of eluent B (25 mM Tris

buffer, pH 8.0, containing 1.0 M NaCl) while decreasing eluent A until, after 2.5 minutes (elapsed time = 3.5 minutes), the mixture is 68% eluent A and 32% eluent B.

Figure 3-13. E1 and E2 Curves

3-20

Begin a 2.5-minute con cav e se gment in which t he amount of eluen t A

is decreased and the amount of eluent B is increase d until the mixtu re is 100% eluent B (elapsed time = 6 minutes).

At 7 minutes, begin a 3-minute conca ve segment, r educing elue nt B to

0% and increasing eluent A to 100% (total elapsed time = 10 minutes).

1. Go to the

METHOD

screen and enter a method number in the

field (3, for example).

If Method 3 currently e xis ts and you w ant to ret ain it, e nter a ne w,

unused method number in the If Method 3 currently exists and you want to delete it, move the

cursor to

2. Move to the

TIME = INIT

field of the

FLOW

and press

INIT

field.

EDIT

Delete

twice.

step and enter a flow rate of 1.50.

Doc. 031377 -03 7/01

EDIT

3 • Operation and Maintenance

Figure 3-14 illustrates the

METHOD

screen as it appears so far. You can now be gi n ent ering th e method step s that will gene rate the curv ed gradient profile (see Figure 3-13).

M ETHO D E DIT

SAVE TO RUN 033

LIMITs 0 - 5000

TIME %A

100.0

%B %C %D V

C FLOW

L1.50IN IT

0.00

Help Message

Figure 3-14. Curved Gradient Method Example (After Step 2)

3. Move the cursor to the

field of the

TIME = 0

step and press

100% of eluent A is automatically filled in.

4. Move the cursor down to the next line and enter 1 in the and then move to the

field and enter 100.

This marks the end of the isocratic section of the run and the beginning of the eluent B concentration ramp. At this point, the concentration of eluent A begins to decrease from 100% as the concentration of eluent B begins increasing from 0%.

PSI

> >

TIME

Enter

field,

Doc. 031377-03 7/01

5. Move the cursor down to the next line and enter 3.5 in the Move the curso r to

and enter 68. Move the cursor to %B and enter

TIME

32.

6. Move the cursor to the

field and enter 3 to run gradient curve 3.

After a total of 3.5 minutes (1 minute of isocratic conditions plus 2.5 minutes to gradually decrease the amount of eluent A in the mixture while increasing the amount of eluent B), the eluent composition is 68% eluent A and 32% eluent B. Figure 3-15 illustrates the screen as it appears after Step 6.

field.

METHOD

3-21

GP50 Gradient Pump

M ETHO D E DIT

TIME %A

100.0

0.00

100.0

%B %C %D V

SAVE TO RUN 033

LIMITs 0 - 5000

C FLOW

L1.50IN IT

PSI

1.00 100.0

3.50 68.0 32.0 3

Help Message

Figure 3-15. Curved Gradient Method Example (After Step 6)

7. Move the cursor to the next line and enter 6 in the the cursor to

8. Move the cursor to the

and enter 100.

field and enter 8.

TIME

After a total of 6 minutes, the eluent composition is 0% eluent A and 100% eluent B.

9. Move the cursor to the next line and enter 7 in the the cursor to

and enter 100. The eluent composition remains

TIME

unchanged at 100% eluent B for 1 minute.

10. Move the cursor to the next line and enter 10 in the the cursor to

and enter 100. After a total of 10 minutes, the

TIME

concentration of eluent B drops to zero and the concentration of eluent A increases to 100%. Figure 3-16 illustrates the completed

METHOD

screen.

> >

field . Move

field. Move

3-22

M ETHO D E DIT

SAVE TO RUN 033

LIMITs 0 - 5000

TIME %A

3.50 68.0 32.0 3

%B %C %D V

6.00 100.0

C FLOW

1.50

7.00 100.0

10.00 100.0

Help Message

Figure 3-16. Curved Gradient Method Example (Complete)

11. Move the cursor to the

SAVE TO

field and press

Enter

method to memory.

PSI

> >

to save the

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3 • Operation and Maintenance

3.4.4 Editing a Running Method Example

After entering a method, you can modify it by changing, adding, or deleting steps.

The example describes how to make the following changes to Method 2, the linear gradient example (see Section 3.4.2):

Change the eluent composition at

TIME = 15

from 50% eluent B and

50% eluent C to 45% eluent B and 55% eluent C. Add a step to Method 2 at

TIME = 20.0

to make the eluent composition

40% eluent B and 60% eluent C.

Figure 3-17 illustrates the

METHOD

screen as it will appear when editing

is complete. Figure 3-18 illustrates the edited grad ient profile.

M ETHO D E DIT

TIME %A

10.00

15.00

20.00 40.0 60.0

25.00

Help Message

65.0 35.0

Figure 3-17. Edited Linear Gradient Method Example

%B %C %D V

45.0 55.0

SAVE TO RUN 222

LIMITs 0 - 5000

C FLOW

2.00

100.0

PSI

> >

Doc. 031377-03 7/01

Figure 3-18. Gradient Profile After Editing

3-23

GP50 Gradient Pump

This example assumes that the example Method 2 is currently running.

1. Go to the

METHOD

screen and enter 2 in the

EDIT

field.

2. Move the cursor down through Method 2 until you reach the

TIME = 15

3. Move the cursor to the time field. Move the cursor to

4. Move the cursor to the

5. Press

step. Move the cursor to %B and enter 45. Move the cursor

and enter 55.

field and press

TIME

and enter 4 0. Move the cursor to

. Enter 20 in the

Insert

and enter 60.

and select either the

SAVE TO

field and press

screen or

MAIN

Enter

DETAIL

screen. Check

the status of the method clock: If the elapsed time is less than 15 minutes (the time for the first

change made to the method), the changes will be incorporated into this run and executed at the programmed time.

If the elapsed time is gr ea te r th an 15 minutes, the changes will not be

incorpora ted into this run. To put the changes into effect, either press

to set the method clock to the

Reset

elapsed time in the

MIN

field that is less than 15 (10, for example).

ial conditions, or enter an

INIT

The method will restart, using the parameters programmed for

TIME = 10

, and the method changes will be incorporated at the

programmed time.

3-24

Doc. 031377 -03 7/01

3.5 Routine Maintenance

This section describes routine maintenance procedures to be performed by the user. Any other maintenance procedures must be performed by qualified Dionex personnel.

3.5.1 Daily Maintenance

When using a combination of eluents which contain both salt or base

and solvent, rinse the piston seals frequently or continuously. Eluent tends to crystallize as the solvent e vaporates; these crystals can abrade the pistons and cause the piston seals to leak. Rinse the piston seals before and after operation each day as described in the following steps, or install a continuous seal wash kit (P/N 059187), to rinse the piston seals continuously (see Section B.2.8).

1. Open the lower pump door and locate the two rinse ports on the front of each of t he pump heads. Figure 3-19 shows the rinse port connections.

2. Install a rinse waste tube (P/N 054418), provided in the GP50 Ship Kit, onto each head (see Figure 3-19).

3 • Operation and Maintenance

Rinse Waste

Doc. 031377-03 7/01

Tu bes

Figure 3-19. Rinsing the Piston Seals

Rinse Ports wi th F ema le Luer Adapters

Syringe (P/N 054578)

3-25

GP50 Gradient Pump

3. Place the end of each rinse waste tube into a waste receptacle. Attach a small syringe (P/N 054578) containing 5 to 10 mL of deionized water to the rinse inle t female luer adapt er on one of the pump heads.

4. Inject deionized water into the fitting to rinse the pump heads.

5. Repeat Steps 3–4 for the other pump head.

6. Dispose of the waste water and close the door to the mechanical chassis.

Check the entire mechanical chassis for leaks from the rinse ports, the

eluent manifold connectio ns and valves, the vac uum deg a s chambers , and the eluent reservoirs (see Figure 3-20). Tighten or replace any leaking fittings. Wipe up liquid spills and rinse dried reagents off the pump components with deionized water.

Dionex recommends th oro ughly flu shing t he vacuum degas assembl y

with deionized water after each use. Flushing chemicals out of the degas chambers and tubing avoids crystallization in the membrane pores.

3-26

Eluent Reservoirs

VAC Chamber IN

VAC Chamber OUT

ABCD

Proportioning Valve/

Manifold Assembly

Pressure Transducer

Pump Heads

OUT

Figure 3-20. Eluent Flow Schematic

Mixer To Column

Rinse Ports

Priming Block

Doc. 031377 -03 7/01

3.5.2 Periodic Maintenance

Replace both the primary and rinse seals in each pump head

approximately every 6 months. The seals may need to be replaced more often if you rout inely run high pr essur es or h igh fl ow rates, or if you operate the pump continuously.

3.6 Shutdown

Rinse the pump piston seals before and after daily operation to prevent build-

up of salt crystals or oth er cont aminants that can damage the piston seal s (s ee Section 3.5.1).

Before a shutdown of three days or more, flush the system with deionized

water to prevent contaminants from building up. Or, if this is not possible, maintain a continuous rinse through the system until you resume normal operation. Select a fl ow rate of 0.04 mL/min for stand ar d bor e pump he ads or

0.01 mL/min for microbore pump heads, and set all four valves in the eluent

manifold to 25% so that the valves are also flushed.

Flushing the eluent manifold is extremely important if your eluents have a combination of salt or base and solvent. If salt precipitates in the valves, the valve diaphragms may be seriously damaged. If this happens, you will have to replace the entire valve assembly.

3 • Operation and Maintenance

Before a shutdown of more than three days, reduce the pressure on the eluent

reservoir(s) to approximately 21 KPa (3 psi). Shut down the pump by turning off the main power on the GP50.

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3-27

GP50 Gradient Pump

3-28

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4 • Troubleshooting

This chapter is a guide to troubleshooting minor problems that may occur while operating the GP50 Gradient Pump. To use this gu ide , t urn to the section that b est describes the operating problem. There, possible causes of the problem are listed in order of probability.

If you are unable to resolve a problem, contact Dionex Technical Support. In the U.S., call 1-800-346-6390. Outside the U.S., call the nearest Dionex office.

4.1 Left-Right Pump Head Pressure Fluctuations

The GP50 display updates the pressure readout once per piston stroke. A variation of more than 3% from one stroke to the next indicates a problem.

Pump out of prime; there is no eluent

1. Refill the eluent reservoirs. Also make sure that each eluent line extends to the botto m of the reservoir.

2. Reprime the pump (see Section B.2.8).

Pump out of prime; eluents are improperly degassed

1. If the GP50 is not equipped with the optional vacuum degas pump

2. If the GP50 is equipped with the optional vacuum degas pump assembly,

Pump out of prime; end-line filter is dirty or clogged

1. Replace the filter (P/N 045987).

2. Reprime the pump (see Section B.2.8).

Doc. 031377-03 7/01

assembly, degas the eluents manually (see Section 3.1 .1). Reprime the pump (see Section B.2.8).

test the degas p ump: a. Open the

Select

b. The degas pump should turn on and run for about 2 minutes. If it does

not run (see Section 4 .6).

DEGAS STATUS

button to toggle the

screen (press

field to

TEST

Menu, 8, 8

RUN

, and 3). Press a

and press

Enter

4-1

GP50 Gradient Pump

Pump out of prime; blockages in inlet tubing

Kinked or clogge d tubin g cause s the pu mp to be “st arv ed ” for e luent . Replac e the tubing and fittings. Reprime the pump (see Section B.2.8).

If priming the pump does not eliminate excessive pressure fluctuations, the piston seals or check valves may be dirty or defective

1. Follow these steps to isolate the cause: a. If leaks ar e seen from the piston rinse tubes, replace the piston seals

(see Section 5.2).

b. If no leaks are seen, replace the check valves (see Section 5.1).

Impurities in the eluents can cause dirty or defective check valves. Install end line filters (P/N 045987) to help prevent this (see Section 3.1.2).

c. Using a 7-mm open-end wrench or your fingers, loosen the lock on

the mechanical chassis drawer. The lock is on the lower right side of the chassis, between valves 3 and 4 (see the label on the inside of the lower door). Pull out the mechanical chassis drawer a few inches.

d. If a piston does not move when there is pump flow, examine it for

breakage and replace if necessary. If a piston moves, examine the pump head for scratches and replace if necessary (see Section 5.3). If a piston moves slightly and then breaks contact with the rocker arm follower, replace the piston seal (see Section 5.2).

e. Push the mechanical chassis drawer back in place, making sure the

cables are not pinched. Tighten the drawer lock.

Observe the warning label on the inside of the lower door. The arrows on the label indicate moving mechanical parts that present pinch hazards when the pump is on and the mechanical drawer is open. Do not touch any parts within the mechanical chassis while the pump is on.

4-2

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4.2 Pump Will Not Start

Flow rate is set to zero

Reset the flow rate (see Section 2.4.2).

Pump starts briefly during priming, but then stops because of high pressur e li mi t

Open the pressure transduce r waste valve (see Figure B-5) by turning the knob counterclockwise two turns.

4.3 Pump Stops

Method or other remote input instructed the pump to stop

Check the display screen for error messages. If none are displayed, the pump was probably instructed to stop by the method, computer, or other remote signal source.

Electrical cables improperly installed

4 • Troubleshooting

1. Set the pump to the pump.

2. If a non-zero flow rate is displayed and the keypad LED is on, verify that the electrical cables in the mechanical chassis are properly installed.

a. Turn off the GP50 power. b. Using a 7-mm open-end wrench or your fingers, loosen the lock on

the mechanical chassis drawer. The lock is on the lower right side of the chassis, between valves 3 and 4 (see the label on the inside of the

lower door). c. Pull out t he mechanical cha ssis drawer a few inches. d. Locate the distribution card on the top of the mechanical chassis.

Check that all cables are seated correctly in the connectors on the

card. e. Push the mechanical chassis drawer back in place, making sure the

cables are not pinched. Retighten the drawer lock. Turn on the power.

LOCAL

mode,

DIRECT CONTROL

. Press

Off/On

to start

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4-3

GP50 Gradient Pump

Low pressure limit was tripped. The following message is displayed:

1. Verify that eluent is present in the channel selected. If the eluent reservoir is empty, refill it or select a ch annel which does have eluent. Prime the pump (see Section B.2.8) before resuming operation.

2. Make sure the waste valve on the pressure transducer (see Figure B-5) is closed by turning the knob on the pressure transducer housing clockwise.

Overtightening the pressure transducer waste valve may damage the valve and the pressure transducer housing.

3. Make sure there are no liquid leaks in the flow system.

Low Pressure Limit Violation

4. Set the pump to

LOCAL

mode,

DIRECT CONTROL

. Press

Off/On

to start the

pump. Verify that the pistons are moving and that you can hear the pump. If there is no sound from the pump, check the LED on the CPU card

inside the door to t h e ele ct ronics chassis. A red LED ind icates a defective power supply. To have the power supply (P/N 046440) replaced, contact Dionex Technical Support.

With the pump running, open the and

). and note whether the left-right pressure varies by more than 3%

DSP STATUS

screen (press

Menu, 8, 8

between strokes. If it does, refer to Section 4.1. If it does not, either increase the flo w rate or reduce the lo w pre ssure limit s etting and cont inue operation.

4-4

Doc. 031377 -03 7/01

4 • Troubleshooting

High pressure limit was tripped. The following message is displayed:

High Pressure Limit Violation

1. Isolate segments of the flow path to determine the source of the high backpressure.

a. Remove the pump inlet tubing from the injection valve. b. Turn on the pump and record the backpressure. c. One at a time, add each segment of the remainder of the flow path to

determine the source of the backpressure. If reconnecting a component causes a sharp increase in backpressure, preplace the component. Replace tubing, fittings, or components as necessary to resume operation at the standard operating backpressure.

d. If the source of the high backpressure is the column, refer to the

column manual for cleanup procedures. The column may need replacement.

2. Verify that the pressure transducer is calibrated correctly. a. Open the pressur e tr ansducer waste v al ve (see Figure B-5) by turning

the knob counterclockwise about two turns.

b. Check the pressure reading; if it is above 97 KPa (14 psi), recalibrate

the pressu re transducer (see Section C.3.6).

A DSP-related error message is displayed

Several error messages are related to Digital Signal Processor (DSP) errors:

DSP communication fails, DSP does not acknowledge

, etc. If one of these

messages is displayed, follow the procedure below:

1. Turn off the GP50 power.

2. Verify that the DSP card is present and correctly installed in slot 1 of the electronics chassis (see Figure 2-6).

3. Turn on the GP50 power.

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4-5

GP50 Gradient Pump

4. If the DSP error message reappears, notify Dionex Technical Support. The power supply (P/N 046440), DSP card (P/N 045369), or CPU card (P/N 046340) may need to be replaced.

Do not remove any of the electronic cards from the pump. There are no user-serviceable components on the cards. If servicing is required, it must be performed by qualified personnel and appropriate electrostatic discharge (ESD) handling procedures must be followed.

The following error message displays:

Motor Drive Fails

4-6

If the pump motor is in a runaway condition, the motor automatically shuts off and the above error message is displayed. Contact Dionex Technical Support.

The following error message displays:

Encoder index not found

1. Turn off the GP50 power.

2. V eri fy tha t th e cable s connect ed to the DSP car d in the elect ronic s chassi s (see Figure 2-6) are seated properly.

3. Turn on the GP50 power. If the error message reappears, notify Dionex Technical Support.

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4.4 Liquid Leaks/Leak Alarm

Leaks from the fr ont rinse po rts or r e ar of the pump hea d may indi cate a defective piston seal

1. Replace the piston seal and the rinse seal (see Section 5.2).

2. Check all connections between the eluent reservoirs and the pump heads. Tighten the fitting connections just enough to stop the leak.

Proportioni ng valve leaks

Tighten loose fittings. If there are no loose fittings, replace the valve (see Section 5.5).

Overtightening the fitting connections may strip the threads in the valve block. If this happens, replace the entire manifold assembly (P/N 046203).

Pressure transducer leaks

Inspect the pressure transducer. If the waste valve is the source of the leak, replace the waste valve O-ring (see Section 5.4). If the leak is from the rear of the transducer, contact Dionex Technical Support.

4 • Troubleshooting

Priming valve

Tighten any leaking fittings just enough to stop the leak. If this does not stop the leak, replace th e fittings and/or tubi ng maki ng the connection. If this does not stop the leak, replace the priming block assembly (P/N 054086). Contact Dionex Technical Support.

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4-7

GP50 Gradient Pump

Interior mechanical chassis leaks

Inspect the chassis for leaks. Tighten any leaking fittings. Replace any damaged parts.

4.5 Noisy Pump Motor

DSP (digital signal processing) card curr ent limit ha s been exc eeded. The card includes a built-in current limiter to protect the motor and motor drive.

Check the three small LEDs in the upper left corner of the DSP card bulkhead, which is in the electronics chassis behind the pump upper door. If the LEDs are flashing in time with the pump strokes, the current limiter is being activated. As the pump motor ages, it becomes less efficient and the current limit is acti v at ed more frequent ly. Activating the current limit is har mle ss, b ut if it occurs frequently, even at low speeds and/or pressures, the bottom plate assembly (P/N 045670) needs to be replaced. Call Dionex Technical Support for assistance.

Pressure servo oscillation

Check the correct pump head volume and head material are selected. If the settings are correct but the problem persists, notify Dionex.

Out of prim e

Reprime the pump (see Section B.2.8).

DSP STATUS

screen (pre ss

Menu, 8, 8

, and 3) to verify that the

4-8

Doc. 031377 -03 7/01

4.6 Vacuum Degas Pump Does Not Run

4 • Troubleshooting

_ DEGAS OPTIONS

Go to the field is set to

DEGAS OPTIONS

screen settings incorrect

screen (pre ss

ALWAYS OFF

, select

BY SETTING

and 4). If the

DEGAS PUMP

and then enter the cycle

duration and frequency times (see SectionC.1.6).

Electrical cables improperly installed

Follow the steps below to manually test the degas pump.

1. Go to the

DEGAS STATUS

button to toggle the

screen (press field to

TEST

RUN

Menu, 8, 8

and press

, and 3). Press a

Enter

2. The pump should turn on and run for the cycle duration time specified in the

DEGAS OPTIONS

screen (2 minutes by default). If it does not run, verify tha t the ca bles conne cted to the pump in the e lectron ics chas sis (see Figure 2-6) and in the mechanical chassis are properly connected.

To check the mechanical chassis connections: a. Turn off the GP50 power. b. Using a 7-mm open-end wrench or your fingers, loosen the lock on

the mechanical chassis drawer. The lock is on the lower right side of the chassis, between valves 3 and 4 (see the label on the inside of the lower door). Pull the drawer out a few inches.

c. Locate the distribution card on the top of the mechanical chassis.

Check that all cables are seated correctly in the connectors on the card.

Select

Doc. 031377-03 7/01

d. If the connections are correct, the distribution card may need to be

replaced. Call Dionex Technical Support.

e. Push the mechanical chassis drawer back in place, making sure the

cables are not pinched. Retighten the drawer lock.

4-9

GP50 Gradient Pump

4.7 Vacuum Degas Pump Calibration Fails

At the end of the degas calibration, the

DEGAS READING

value is less than

13000 counts and one of the following error messages appears:

Degas vacuum pump is not present or degas

circuitry is malfunctioning.

Vacuum Degas Fails

Verify that the cable to the vacuum degas pump is connected to the distribution card in the mechanical chassis.

1. Turn off the GP50 power.

2. Using a 7-mm open-end wrench or your fingers, loosen the lock on the mechanical chassis drawer. The lock is on the lower right side of the chassis, between valves 3 and 4. Pull out the drawer a few inches.

3. The distribution card is on the top of the mechanical chassis. Labels printed on the card identify the various cables plugged into it. Locate the connector for the vacuum degas pump (labeled

VAC PUMP

) near the right rear corner of the card. Make sure th e cable is fully seated in the connector.

4. Push the mechanical chassis drawer bac k in pla ce, mak ing sur e the ca bles are not pinched. Retighten the drawer lock.

4-10

5. Turn on the power.

6. Retry the calibration. If the message reappears, contact Dionex Technical Support.

Doc. 031377 -03 7/01

4.8 Vacuum Degas Pump Low Vacuum

The GP50 monitors the degas vacuum reading every 1 min. If the degas vacuum is lower than the monitoring value, the degas pump turns on. When the pump turns off, if the vacuum reading is 2000 counts or more lower than the monitor ing value, the following message displays:

LOW VACUUM ALARM!!

Check DEGAS OPTIONS settings or refer to

service manual

4 • Troubleshooting

Open the

DURATION

DEGAS OPTIONS

time and/or decreasing the

screen (press

TIME BETWEEN CYCLES

resolve the problem, contact Dionex Technical Support.

4.9 Relays or TTLs Inoperative

Incorrectly installed cables

Make sure the cable(s) between the GP50 relay or TTL connector(s) and the connector(s) on the other instrument(s) are connected properly (see Section D.3).

Method programming error

For Local control, make sure the parameter to turn on or off the relay/TTL is set correctly on the Remote control, make sure the command for turning the relay/TTL on or off is entered correctly into the PeakNet 6 program file or the PeakNet 5 Method.

For TTL inputs, make sure the controlling device is programmed correctly.

When attempting to set TTL2, the following message displays:

TTL2 is set to indicate FLOW/NO FLOW.

METHOD EXTENSION

and 4). Try increasing the

. If this does not

screen (see Section C.1.5). For

CYCLE

The to signal when pump flow stops ( power to a Self-Regenerating Suppressor function, open the

OUTPUT USAGE

Doc. 031377-03 7/01

TTL2 OUTPUT USAGE

PUMP OPTIONS

field to

field on the

0 FLOW

NORMAL

PUMP OPTIONS

screen is currently set

). This setting is used to control the

(SRS®). To use TTL2 for another

screen (press

and 6) and set the

TTL2

4-11

GP50 Gradient Pump

4.10 Poor Chromatographic Reproducibility

Liquid lines incompletely flushed after an eluent change

Attach a syringe to the prim ing block v al v e (see Fig ure B-5) and draw at least

2.5 mL (20 mL if the vacuum degas pump is installed) of the new eluent

through the liquid lines before beginning operation.

Leaking piston seal

Check for liquid leaks at the rinse ports in the front of the pump heads. Replace the piston seal on any head with a leak (see Section 5.2).

Malfunctioning proportioning valve

Test the valves (see Section C.2.7). If a test fails, the prop ortioning valve assembly may need to be replaced (see Section 5.5).

4-12

Doc. 031377 -03 7/01

5•Service

This chapter describes service and repair procedures that the user may perform. Any procedures not included here, including electronics-related service procedures, must be performed by Dionex personnel. For assistance, contact Dionex Technical Support. In the U.S., call 1-800-346-63 90. Outside the U.S., call the nearest Dionex office.

Before replacing an y par t, ref er to t he tr oublesh ooting infor mation i n Chapte r 4 to isolate the cause of the problem.

Substituting non-Dionex parts may impair performance, thereby voiding the product warranty. Refer to the warranty statement in the Dionex Terms and Conditions for more information.

The CPU card contains a lithium battery. If the CPU card is displaced, dispose of the used battery according to the manufacturer's instructions.

5.1 Cleaning and Replacing the Check Valves

A dirty check valve causes erratic flow rates and pressures and may cause the pump to lose prime and/or be difficult to reprime.

1. Turn off the main power switch, to ensure you do not unintentionall y st ar t t he

pump.

2. Disconnect the tube fittings from the inlet and outlet check valve housings

(see Figures 5-1 and 5-2).

3. Use a 1/2-inch wrench to loosen both check valve housings. Remove the

check valve housings and cartridges from the pump head. Carefully remove the check valve cartridges from the housings.

4. Place the check valve housings and cartridges in a beaker with methanol, and

sonicate or agitate for several minutes.

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5-1

GP50 Gradient Pump

Outlet Check

Pressure Transducer

Valv e

To Column

Pump Head

Inle t

Check

Valv e

Figure 5-1. Pump Heads and Liquid Lines (PEEK)

Pressure Transducer

To C o lu mn

To Waste

5-2

Outlet Check

Valve

Inle t

Check

Valve

Figure 5-2. Pump Heads and Liquid Lines (SST)

Pump Head

To Waste

To Eluent Manifold

Doc. 031377 -03 7/01

5•Service

5. Rinse each check valve housing and cartridge thoroughly with filtered

deionized water.

6. The

check val v e assembly housing has a 1/4-28 port (PEEK pumps) or a

inlet

10-32 port (stainless steel pumps). Replace the cartridge in the inlet check valve housing, making sure the double-hole end of the cartridge is visible.

The

check valve assembly housing has a 10-32 port (PEEK and

outlet

stainless steel pumps ). Replace the ca rtridge in th e outlet check va lve housi ng, making sure the single-hole end of the cartridge is visible. Liquid flows through the check valve in the large single hole and out the small double holes.

NOTE The pump will not operate properly unless the cartridge

is installed in the housing in the correct orientation.

7. Reinstall the check valves. Be sure to install the inlet check valve on the

bottom of the head and the outlet check valve on the top of the head. Tighten only enough to seat (25 in-lb. torque). Tighten a little more only if it leaks.

Overtightening may damage the pump head and check valve housing and crush the check valve seats.

8. Reconnect the liquid lines.

9. Turn on the GP50 power.

10. Prime the pump (see Section B.2.8). If the pump will not prime and you have eliminated all other possible causes of the problem, replace the check valve cartridge.

After replacing the check valve cartridge, go to the (press

Enter

Doc. 031377-03 7/01

Check Valve Cartridge Type Part Number

Standard Bore PEEK 047747 Standard Bore Stainless Steel 047755 Microbore PEEK 047748 Microbore Stainless Steel 048279

Menu, 8

, and 2). Move the cursor to the

VALVES IN USE

to reset the field to 0 cycles.

ELAPSED TIME

field and press

screen

5-3

GP50 Gradient Pump

5.2 Piston Seal Replacement

A damaged seal allows leakage past the piston and then through the rinse ports in the front of the pump heads. The pump may be difficult to prime, flow rates will be unstable, and baseline noise may be observed.

1. Turn off the main power switch, to ensure you do not unintentionall y st ar t t he GP50.

2. Disconnect the tube fittings from the pressure transducer and the inlet check valve (see Figures 5-1 and 5-2).

3. Remove the two nuts from the pump head.

4. Carefully disengage the head from the piston by pulling the head straight off and away from its mounting guides.

Lateral motion while disengaging the head fr om the piston ma y break the piston.

Un mouvement latéral pendant la séparation de la tête et du piston peut casser le piston.

5. Place the head (front end down) on a clean work surface and lift off the backup washer to expose the piston guide (see Figures 5-3 and 5-4).

6. The pistons are captured by a magnetic retention system and do not come off as part of the pump head assembly. After removing the pump head, hold the shaft of the piston and apply just enough lateral force to overcome the magnetic field and remove the piston.

5-4

Doc. 031377 -03 7/01

Piston Seal

Outlet Check

Valve Assembly

Head

5•Service

Rinse Seal

O-Ring

Support Washer

Backup Was her

Piston

Piston Guide

Inlet Check Valve Assembly

Figure 5-3. Pump Head Assembly

Component Standard

Bore/PEEK

Standard Bore/SST

Microbore/ PEEK

Microbore/ SST

Pump Head Assembly 054087 054096 054094 054095

Pump Head 054051 054050 054098 054099 Outlet Check Valve 047661 047665 047657 047663 Inlet Check Valve 047660 047665 047656 047663 Piston Seal 054400 054402 054401 054401 Piston Guide 045633 045633 045632 045632 Rinse Seal 048722 048722 048721 048721 Support Washer 050745 050745 050744 050744 O-Ring 014895 014895 014895 014895 Backup Washer 045630 045630 045631 045631 Piston 052840 052840 053584 053584

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Table 5-1. Pump Head Assembly Part Numbers

5-5

GP50 Gradient Pump

7. To remove the piston guide and seal: a. Fill the head cavity with deionized water by injecting through either the

piston opening or the inlet check valve. b. Reinsert the piston approximately 1/8-in into the seal (see Figure 5-4). c. Install a 10-32 fitting plug (P/N 042772) on the outlet check valve.

Tighten the plug. d. Push the piston into the head. This should hydraulically unseat the seal

and piston guide from the head. Remove the piston and pull off the guide

and seal.

NOTE If the piston guide an d seal do no t come out, m ake sure

the 10-32 plugs are tight. Then, add more water and repeat Steps b and d.

e. Remove the 10-32 fitting plug.

10-32 Fitting Plug

Outlet

Check

Valv e

Pump

Head

(P/N 042772)

Piston

Push in to unseat the seal and piston guide.

Piston

Seal

Remove the pisto n from the h e a d and pu ll off the seal and piston guide.

Guide

5-6

Inlet Ch e c k

Valv e

Fitting P lug (Optional)

Backup Washer (Remove)

Figure 5-4. Removing the Piston Seal

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5•Service

8. To install the new seal and reinstall the piston guide: a. Push the pisto n through the pist on guide an d the new seal. Then, inser t the

piston, piston guide, and seal into the pump head until the seal contacts the bottom of the counterbore (see Figure 5-5, View A).

b. Hold the piston guide and seal in place and remove the piston from the

head (see Figure 5-5, View B).

c. Seat the seal by pu shing the pist on guide i nto the he ad unt il it is flush wit h

the head.

View A

Seal

Piston Guide

Piston

Pump Head

Push the piston through the seal an d g u ide and pa r tially ins e r t in to the head, just until the seal contacts the coun terbore.

Counterbore

View B

Remove the piston and push the pis to n guid e in to the h ead t o fin ish seating the seal.

Figure 5-5. Installing the Piston Seal

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5-7

GP50 Gradient Pump

9. Dionex recommends reinstalling the head and piston as a single assembly so that the piston centers itself. To do this:

a. Press the backup washer into the head until it is flush with the indented

surface of the head.

b. Insert the piston

halfway

into the head. This ensures that the magnet in th e follower picks up the piston. (The follower is the cylinder that holds the piston in place as it moves in and out of the pump head assembly.)

c. Reinstall the he ad and p iston assembl y, using a wrench to tighten the nuts

evenly (12 in-lb torque).

10. Reconnect the liquid line to the inlet check valve.

11. Reconnect the tube fittings to the pressure transducer.

12. Turn on the GP50 power.

13. Go to the the

SEALS IN USE

ELAPSED TIME

field to 0 cycles. The pump is ready for normal operation.

screen (press

5.3 Pump Piston Replacement

Continued leaking through the rinse ports after replacing the piston seal (assuming the head is tight) indicates a dirty, scratched, or broken piston.

1. Turn off the main power switch, to ensure you do not unintentionall y st ar t t he GP50.

2. Disconnect the tube fittings from the pressure transducer and the inlet check valve (see Figures 5-1 and 5-2).

Menu, 8

, and 2). Press

Enter

to reset

5-8

3. Remove the two acorn nuts from the pump head.

Lateral motion while disengaging the head fr om the piston ma y break the piston.

Un mouvement latéral pendant la séparation de la tête et du piston peut casser le piston.

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5•Service

4. Slowly pull the head and allow it to separate from the housing. Carefully disengage the head from the piston by pulling the head straight off and away from the mounting guides.

5. The pistons are captured by a magnetic retention system and do not come off as part of the pump head assembly. After removing the pump head, hold the shaft of the piston and apply just enough lateral force to overcome the magnetic field and remove the piston.

6. Replace the piston and the piston seal. Broken or scratched pistons can damage the piston seal.

7. Dionex recommends reinstalling the head and piston as a single assembly so that the piston centers itself. To do this:

a. Press the backup washer into the head until it is flush with the indented

surface of the head.

b. Insert the piston

halfway

into the head. This ensures that the magnet in th e follower picks up the piston. (The follower is the cylinder that holds the piston in place as it moves in and out of the pump head assembly.)

c. Reinstall the he ad and p iston assembl y, using a wrench to tighten the nuts

evenly (12 in-lb torque).

8. Reconnect the liquid line to the inlet chec k valve.

9. Reconnect the tube fittings to the pressure transducer.

10. Turn on the main power switch and prime the pump (see Section B.2.8).

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5-9

GP50 Gradient Pump

5.4 Pressure Transducer Waste Valve O-Ring Replacement

A damaged O-ring causes leakage aro und the bas e of the pre ssur e transdu cer waste valve knob.

1. Turn off the main power switch, to ensure you do not unintentionall y st ar t t he

GP50.

2. Remove the valve from the pressure transducer housing by turning the knob

counterclockwise until it comes loose from the housing.

3. Remove the O-ring (P/N 046434) (see Figure 5-6).

4. Carefully slide a new O-ring (P/ N 046434) over the end of the valv e and push

it into the groove.

5. Reinstall the valve in the housing, turning the knob clockwise until the valve

is seated.

Do not overtighten the waste valve.

5-10

O-Ring

(P/N 046434)

Needle Valve (P/N 042625)

Screw (4)

(P/N 053988)

Transducer Cable Assembly

Gasket Support

(P/N 054065)

Transducer Housing (P/N 054052)

Washer (4) (P/N 045689)

(P/N 054064)

Gasket (P/N 054417)

Figure 5-6. Pressure Transducer Assembly

Transducer Sleeve (P/N 054053)

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5.5 Proportioning Valve Replacement

A defective propor tioning valve can cause the following problems: leak s, nonrepr oducible eluent co mpositions (whic h may cause r etention time shi fts), and flow restrictions (which may cause high backpressure).

1. Turn off the main power switch, to ensure you do not unintentionall y st ar t t he GP50.

2. Turn off the pressure on the eluent reservoirs and allow them to vent.

3. Follow these steps to disconnect the proportioning valve electrical connector from the distribution card.

a. Using a 7-mm open-end wrench or your fingers, loosen the lock on the

mechanical chassis drawer. The lock is on the lower right side of the chassis, between valves 3 and 4 (see the label on the inside of the lower door). Pull out the drawer a few inches.

b. The distribution card is on the top of the mechanical chassis. Labels

printed on the card identify the various cables plugged into it. Locate the proportioning valve electrical connector, labeled the card. Dis connect it from the card.

VALVES

5•Service

, at the front of

4. Disconnect the liquid line from the manifold outlet (see Figure 5-7).

Figure 5-7. Location of Proportioning Valve/Manifold Assembly

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Manifold

Outlet

Proportioning Valve/

M a n ifold A s sem bly (P/N 046203)

5-11

GP50 Gradient Pump

5. Loosen the screws securing the proporti oning valve/ manif ol d assembly to the bulkhead (see Figure 5-7). Pull the valve/manifold assembly forward and disconnect the eluent lines on the manifold inlet. Remove the valve/manifold assembly completely from the bulkhead.

6. Thread the bundle of electrical lines from the new valve/manifold assembly through the bulkhead and up to the distribution card. Connect the valve electrical connector to the

7. Connect the eluent lines to the new valve/manifold assembly. Make sure eluent lines A through D are in the appropriate valve ports. Connect eluent line A to the valve marked #1, eluent B to the valve marked #2, and so on.

Tighten liquid connections to the valve no more than fingertight plus one-quarter turn. Overtightening or crossthreading the valve fittings may strip the threads in the manifold block. If this happens, replace the valve/manifold assembly.

8. Align the new assembly as shown in Figure 5-7 and mount it to the bulkhead. Tighten the screws.

VALVES

connector on the distribution card.

9. Push the mechanical chassis drawer back in plac e, maki ng sur e th e cabl es are not pinched. Retighten the drawer lock.

10. Attach the liquid line from the priming block to the manifold outlet.

11. To verify that each valve is functioning properly, connect a syringe to the priming valve. One at a time, select 100% of each eluent valve and draw liquid through the valve. After successfully drawing liquid through each valve, turn off the pump.

12. Next, veri fy that no liquid passes through the valve when th e pump is off. Do not turn on the pump. One at a time, select 100% of each eluent. No liquid should flow through the valve.

13. Turn on the main power switch. The pump is ready for normal operation.

5-12

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5.6 Changing Main Power Fuses

1. Turn off the main power.

HIGH VOLTAGE—Disconnect the main power cord from its source and also from the rear panel of the GP50.

HAUTE TENSION—Débranchez le cordon d'alimentation principal de sa source et du panneau arrière du GP50.

2. The fuse holder is part of the main power receptacle on the rear panel (see Figure 5-8). Note the recessed lock located on each side of the fuse holder. Using a small screwdriver, push each lock toward the center to release it. When both locks are released and the fuse holder pops out slightly, pull the fuse holder straight out of its compartment.

3. Replace the two fuses in the holder with new IEC127 fast-blow fuses rated

3.15 amps (P/N 054745). Dionex recommends always replacing both fuses.

5•Service

4. Reinsert the fuse holder into its compartment. The fuse holder is keyed to fit only in its proper orientation. Apply enough pressure evenly against the holder to engage the two locks. When both locks are engaged, the holder is flush against the panel.

5. Reconnect the main power cord and turn on the power.

Figure 5-8. Main Power Fuse Holder

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5-13

GP50 Gradient Pump

5-14

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A.1 Electrical

A • Specifications

Main Power

Requirements

Fuse

Requirements

A.2 Environmental

Operating

Temperature

Operating

Humidity

A.3 Physical

Dimensions

Weight

Decibel Level

100 Vac to 240 Vac, 50/60 Hz; 2.5 amps. The GP50 power supply is main voltage auto-sensing and requires no manual adjustment.

Two 3.15 amp fast-blow IEC127 fuses (P/N 954745)

10 °C to 50 °C (50 °F to 104 °F)

5 to 95% relative humidity (non-condensing)

33.5 cm high x 22.5 cm wide x 42 cm deep (13.1 in x 8.9 in x 16.8 in) 6 cm (2.5 in) clearance required behind the module

19 kg (42 lb) 60 db (“A WEIGHING” setting)

A.4 Display and Keypad

Display

Keypad

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Liquid crystal with adjustable backlighting. 26-button pad for entering commands and numeric values for

screen parameters.

A-1

GP50 Gradient Pump

A.5 Hydraulics

Eluent

Selection

Pump

Flow Rate

Operating

Pressure Pressure

Resolution

High Pressure

Limit

Low Pressure

Limit

Delay Volume

(No Mixer)

Four differ ent eluent componen ts; each can be pr oportioned from 0 to 100%

Dual-piston, variable speed, 100 µL (standar d bore), 25 µL (microbore) stroke, user-selectable constant pressure or constant flow feedback control

Standard bore pump head: 0.04 to 10.0 mL/min linearly variable in increments of 0.01 mL/min

Microbore pump head: 0.01 to 2.50 mL/min linearly variable in increments of 0.01 mL/min

35 MPa (5000 psi) maximum

0.07 MPa (10 psi)

0 to 35 MPa (0 to 5000 psi) in increments of 0.05 MPa (7.2 5 psi); trips instantaneously

0. to 35 MPa (0 to 5000 psi) in increments of 0.05 MPa (7.25 psi); trips after a time -ou t of 0.4 mL fo r the stan dard bore GP50 or 0.1 mL for the microbore GP50

Standard bore pump head: <700 µL Microbore pump head: <300 µL

A-2

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A.6 Control

A • Specifications

Remote

Local (Front Panel)

Limited remote operation via TTL-input logic level, and TTLoutput and relay contact closures, or full remote programming and control via the DX-LAN interface.

Methods:

The actual number of stored methods depends on available memory. Each method may contain up to 50 separate steps.

Storage:

programs during power-down or in the event of a power failure.

Stores up to 100 separate methods (00 through 99),

Nonvolatile memory protects against the loss of

A.7 Vacuum Degas Pump Assembly (Optional)

Channels

Materials

4-channel membrane vacuum degas Wetted materials, PEEK, PTFE

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A-3

GP50 Gradient Pump

A-4

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B.1 Facility Requirements

Make sure the GP50 installation site meets the electrical and environmental

requirements in Appendix A. Install the GP50 on a sturdy workbench at a height that ensures convenient

viewing of the front panel display and access to the interior.

Lift the GP50 only from the bottom or side surfaces of the module. Lifting by the front doors will dama ge the door hinges. Use caution when lifting the module, which weighs 19 kg (42 lb).

Ne soulevez le GP50 que par le fond ou les côtés. Son soulèvement par la porte du panneau avant endommagera les charnières de la porte. Soyez prudent lorsque vous soulevez le GP50: il pèse 19 kg (42 lb).

Allow at least 6 cm (2.5 in) free space behind the GP50 for connections and

ventilation.

B • Installation

The GP50 Gradient Pump is capable of operation with or without head

pressure on the eluent . If pre ssurization is used, provide a source of helium to pressurize the eluent and regenerant reservoirs (if used).

House eluents at least 3 cm (8 in) above the pump i n an EO1 Eluent Org anizer

(P/N 044125) or in built-in eluent containment (such as the LC25 Chromatography Oven reservoir container).

Always filter eluents to remove small particulates that may contaminate the

pump. Install an end-line filter (P/N 045987) on the end of each eluent reservoir line. Filters are supplied in the pressurizable reservoir Ship Kits. Refer to the

Dionex strongly recommends degassing eluents. If the vacuum degas

assembly is not installed, re fer to Section 3.1.1 for manual degassing instructions.

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Pressurizable Reservoir Installation Instructions

for details.

B-1

GP50 Gradient Pump

B.2 Installation Instructions

The GP50 Ship Kit contains items necessary for completing the installation.

GP50 Gradient Pump Version Ship Kit

Standard bore/microbore with PEEK components P/N 054621 Standard bore/microbore with stainless steel components P/N 054627

B.2.1 Power Connection

SHOCK HAZARD—To avoid electrical shock, a grounded receptacle must be used. Do not operate or connect to AC power mains without an earthed ground connection.

DANGER D'ÉLECTROCUTION - Pour éviter toute électrocution, il faut utiliser une prise de courant avec prise de terre. Ne l'utilisez pas et ne le branchez pas au secteur C.A. sans utiliser de branchement mis à la terre.

The power supply cord is used as the main disconnect device. Make sure the socket-outlet is located near the GP50 and is easily accessible.

Le cordon d'alimentation principal est utilisé comme dispositif principal de débranchement. Veillez à ce que la prise de base soit située/installée près du module et facilement accessible.

Operation at AC input levels outside of the specified operating voltage range may damage the GP50.

B-2

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B • Installation

The GP50 power supply is auto-sensing, so no adjustment is required to select the line voltage. There are two ways to control power to the GP50.

Control from the GP50

To implement:

Connect a modular power cord (IEC 320 C13) from the GP50 main power receptacle (see Figure B-1) to a grounded, single-phase power source. Use the GP50 power switch to turn the pump power on and off.

Control from the LC30 Chromatography Oven

To implement:

Locate one of the IEC jumper cables (P/N 960748) provided in the LC30 Ship Kit. Connect the jumper cable from the GP50 main power receptacle to an IEC auxiliary receptacle on the LC30 rear panel. Leave the GP50 power switch on continuously and use the LC30 main power switch to turn the pump power on and off.

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Figure B-1. GP50 Rear Panel

B-3

GP50 Gradient Pump

B.2.2 Electronics Chassis Connections

The electronics chassis connections vary, depending on which chromatography module is installed with the pump. Refer to the appropriate section below.

LC10 or LC20 Connections

1. Route the leak sensor cable from the rear of the LC10 Chromatography Organizer or the LC20 Chromatography Enclosure through the GP50 upper chase (see Figure B-1). Connect the cable to the

LC LEAK

Figure B-2).

2. Route the solenoid valve cable from the rear of the LC10 or LC20 through the GP50 upper chase. Connect the cable to the connector in slot 1 of the pump electronics chassis (see Figure B-2).

NOTE Refer to Appendix D for TTL and relay installation

instructions.

connector in slot 1 of the pump electronics chassis (see

LC AIR

Connect the leak sensor cable from the LC10 or LC20 here

Connect the serial interface cable from the LC30 here

Connect the solenoid valve cable from the LC10, LC20, LC25, or LC30 here

B-4

PWR SPY

L C

L E A K

GP50/IP25-DSP130W

MOTOR

L C

C O M M

DIST

L C

A I R

SLOT 2SLOT 1 BLANK

D I S T

1 3

P I N

D I S T

1 2

P I N

SLOT 3

Figure B-2. GP50 Electronics Chassis

(Located behind pump upper door)

BLANK

SLOT 4

LAN-000K

RLY-1 OUT

RLY-2 OUT

TTL-1

OUT

TTL-2

OUT

TTL-1

TTL-2

TTL-3

TTL-4

POWER SUPPLY GREEN - OK RED - FAULT

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SLOT 5

CPU/RLY

F R O N T

P A N E L

B • Installation

LC25 Connections

Route the electronics injection valve cable from the rear of the LC25 Chromatography Oven through the GP50 upper chase(see Figure B-1). Connect the cable to the electronics chassis (see Figure B-2).

connector in slot 1 of the pump

LC AIR

NOTE Do not connect the LC25 to the

in slot 1; the oven contains internal leak control electronics.

LC30 Connections

LC LEAK

connector

1. Route the RJ-11 serial cable from the rear of the LC30 Chromatography Oven through the GP50 upper chase (see Figure B-1). Connect the cable to the

LC COMM

connector in slot 1 of

the pump electronics chassis (see Figure B-2).

2. Route the solenoid valve cable from the rear of the LC30 through the GP50 upper chase. Conne ct the c able to t he

connector in slot 1

LC AIR

(see Figure B-2).

NOTE Do not connect the LC30 to the

in slot 1; the oven contains internal leak control electronics.

LC LEAK

connector

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B-5

GP50 Gradient Pump

B.2.3 DX-LAN Interface: 10BASE-T Connections (Optional)

NOTE Check the DX-LAN connector on the GP50 rear

panel. If a 10BASE-T RJ-45 (telephone-style) connector is installed, follow the instructions in Section B.2.3. However, if a BNC connector is installed, follow the instructions in Section B.2.4.

In order to communicate with a host computer running Dionex chromatography software, the GP50 must contain a pump interface card (P/N 056800) and an unshielded twisted-pair 10BASE-T DX-LAN cable (P/N 960281) must be connected from the 10BASE- T RJ-45 connector on the rear panel to a “combo” 10BASE-T Ethernet hub (P/N 056909).

Installing or Replacing the Pump Interface Card

NOTE If the DX-LAN option was not installed at the

factory, order the pump interface card kit (P/N 057005). The kit includes all the components required for DX-LAN communication.

B-6

STATIC— The GP50 electronics cannot be serviced by the user. The pump interface card must be installed by qualified personnel. Standard anti-static procedures must be observed when installing the interface card or handling the CPU card.

To prevent damage to the GP50, turn off the main power before installing the pump interface card. After confirming that the LED on the CPU card is off (not green or red), unplug the power. Do not rely on the front panel power switch.

Pour éviter d'endommager le GP50, coupez l'alimentation électrique principale avant d'installer la carte interface du pompe. Après avoir confirmé que la DEL de la carte d'unité central est étein te (ni ver te ni rouge), débranchez le courant. Ne vous fiez pas à la position de l'interrupteur d'alimentation du panneau avant.

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Dionex GP50 Operator's Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Contents

1.1 Overview

1.2 About This Manual

1.2.1 Saf et y Me ss a ges and Note s

1.2.2 Saf et y Label s

2.1 Front Control Panel

2.1.1 Control Panel Keypad

2.1.2 Display Screens

2.2 Electronics Chassis

2.3 Mechanical Chassis

2.4 Mechanical Components

2.4.1 Pump Eluent Manifold

2.4.2 Pump Heads

2.4.3 Pump Mixers

2.4.4 Pump Priming Block

2.4.5 Pressure Transducer

2.5 Vacuum Degas Pump Assembly (Optional)

2.6 Piston Seal Wash (Optional)

2.7 Eluent Reservoirs

2.8 Rear Panel

2.9 Functional Description

2.9.1 Operating and Control Modes

2.9.2 Local and Remote Modes

2.9.3 Method Control

2.9.4 Eluent Delivery

3.1 Getting Ready to Run

3.1.1 Degas Eluents

3.1.2 Filter Eluents

3.1.3 Pressurize Eluent Reservoirs

3.1.4 Start-Up

3.1.5 Selecting the Pressure Limits

3.1.6 Selecting the Operating and Control Modes

3.2 Running Under Direct Control (Local Mode)

3.3 Running Under Method Control (Local Mode)

3.3.1 Creating a New Method

3.3.2 Running a Method

3.3.3 Editing a Method

3.3.4 Deleting a Method

3.3.5 Changing the Running Method

3.3.6 Controlling the Method Clock

3.4 Example Methods

3.4.1 Isocratic Method Example

3.4.2 Linear Gradient Method Example

3.4.3 Curved Gradient Method Example

3.4.4 Editing a Running Method Example

3.5 Routine Maintenance

3.5.1 Daily Maintenance

3.5.2 Periodic Maintenance

3.6 Shutdown

4.1 Left-Right Pump Head Pressure Fluctuations

4.2 Pump Will Not Start

4.3 Pump Stops

4.4 Liquid Leaks/Leak Alarm

4.5 Noisy Pump Motor

4.6 Vacuum Degas Pump Does Not Run

4.7 Vacuum Degas Pump Calibration Fails

4.8 Vacuum Degas Pump Low Vacuum

4.9 Relays or TTLs Inoperative

4.10 Poor Chromatographic Reproducibility

5.1 Cleaning and Replacing the Check Valves

5.2 Piston Seal Replacement

5.3 Pump Piston Replacement

5.4 Pressure Transducer Waste Valve O-Ring Replacement

5.5 Proportioning Valve Replacement

5.6 Changing Main Power Fuses

A.1 Electrical

A.2 Environmental

A.3 Physical

A.4 Display and Keypad

A.5 Hydraulics

A.6 Control

A.7 Vacuum Degas Pump Assembly (Optional)

B.1 Facility Requirements

B.2 Installation Instructions

B.2.1 Power Connection

B.2.2 Electronics Chassis Connections