Dionex GP50 Operator's Manual
GP50 GRADIENT PUMP
OPERATOR'S MANUAL
© 2001 Dionex Corporation
Document No. 031377
Revision 03
July 2001
©2001 by Dionex Corporation
All rights reserved worldwide.
Printed in the United States of Amer ica.
This publication is protected by federal copyright law. No part of this publication
may be copied or distributed, transm itted, tr anscri bed , stored in a retrieval system, or
transmitted into any human or computer language, in any form or by any means,
electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties
without the express written permission of Dionex Corporation, 1228 Titan Way,
Sunnyvale, California 94088-3603 U.S.A.
DISCLAIMER OF WARRANTY AND LIMITED WARRANTY
THIS PUBLICATION IS PROVIDED “AS IS” WITHOUT WARRANT Y OF
ANY KIND. DIONEX CORPORATION DOES NOT WARRANT,
GUARANTEE, OR MAKE ANY EXPRESS OR IMPLIED
REPRESENTATI ONS RE GARD ING THE U SE, O R TH E RES ULTS OF THE
USE, OF THIS PUBLICATION IN TERMS OF CORRECTNESS, ACCURACY,
RELIABILITY, CURRENTNESS, OR OTHERWISE. FURTHER, DIONEX
CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION
AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT
HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO
NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVI SION OR
CHANGES.
TRADEMARKS
DX-LAN™ is a trademark, and PeakNet®, Self-Regenerating Suppressor®, and
SRS® are registered trademarks of Dionex Corporation
Tefzel® is a registered trademark of E.I. duPont de Nemours & Co.
PRINTING HISTORY
Revision 01, May 1998
Revision 02, October 2000
Revision 03, July 2001
Contents
1 • Introduction
1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
1.2 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
1.2.1 Safety Messages and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
1.2.2 Safety Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
2 • Description
2.1 Front Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
2.1.1 Control Panel Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
2.1.2 Display Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
2.2 Electronics Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8
2.3 Mechanical Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-11
2.4 Mechanical Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-12
2.5 Vacuum Degas Pump Assembly (Optional) . . . . . . . . . . . . . . . . . . . .2-16
2.6 Piston Seal Wash (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-16
2.7 Eluent Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-17
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2.4.1 Pump Eluent Manifold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-13
2.4.2 Pump Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-14
2.4.3 Pump Mixers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-14
2.4.4 Pump Priming Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-15
2.4.5 Pressure Transducer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-15
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GP50 Gradient Pump
2.8 Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-18
2.9 Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-18
2.9.1 Operating and Control Modes . . . . . . . . . . . . . . . . . . . . . . . . 2-18
2.9.2 Local and Remote Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-19
2.9.3 Method Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-20
2.9.4 Eluent Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-22
3 • Operation and Maintenance
3.1 Getting Ready to Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.1.1 Degas Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.1.2 Filter Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.1.3 Pressurize Eluent Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.1.4 Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.1.5 Selecting the Pressure Limits . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
3.1.6 Selecting the Operating and Control Modes . . . . . . . . . . . . . . 3-5
3.2 Running Under Direct Control (Local Mode) . . . . . . . . . . . . . . . . . . . 3-6
3.3 Running Under Method Control (Local Mode) . . . . . . . . . . . . . . . . . . 3-7
3.3.1 Creating a New Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
3.3.2 Running a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-10
3.3.3 Editing a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
3.3.4 Deleting a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
3.3.5 Changing the Running Method . . . . . . . . . . . . . . . . . . . . . . . 3 -12
3.3.6 Controlling the Method Clock . . . . . . . . . . . . . . . . . . . . . . . .3-12
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3.4 Example Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-13
3.4.1 Isocratic Method Example . . . . . . . . . . . . . . . . . . . . . . . . . . .3-13
3.4.2 Linear Gradient Method Example . . . . . . . . . . . . . . . . . . . . .3-14
3.4.3 Curved Gradient Method Example . . . . . . . . . . . . . . . . . . . . .3-19
3.4.4 Editing a Running Method Example . . . . . . . . . . . . . . . . . . .3-23
3.5 Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-25
3.5.1 Daily Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-25
3.5.2 Periodic Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-27
3.6 Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-27
4 • Troubleshooting
4.1 Left-Right Pump Head Pressure Fluctuations . . . . . . . . . . . . . . . . . . . .4-1
Contents
4.2 Pump Will Not Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
4.3 Pump Stops . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
4.4 Liquid Leaks/Leak Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7
4.5 Noisy Pump Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-8
4.6 Vacuum Degas Pump Does Not Run . . . . . . . . . . . . . . . . . . . . . . . . . .4-9
4.7 Vacuum Degas Pump Calibration Fails . . . . . . . . . . . . . . . . . . . . . . .4-10
4.8 Vacuum Degas Pump Low Vacuum . . . . . . . . . . . . . . . . . . . . . . . . . .4-11
4.9 Relays or TTLs Inoperative . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11
4.10 Poor Chromatographic Reproducibility . . . . . . . . . . . . . . . . . . . . . . .4-12
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GP50 Gradient Pump
5•Service
5.1 Cleaning and Replacing the Check Valves . . . . . . . . . . . . . . . . . . . . . . 5-1
5.2 Piston Seal Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
5.3 Pump Piston Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
5.4 Pressure Transducer Waste Valve O-Ring Replacement . . . . . . . . . . 5-10
5.5 Proportioning Valve Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . .5-11
5.6 Changing Main Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-13
A • Specifications
A.1 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1
A.2 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1
A.3 Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1
A.4 Display and Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1
A.5 Hydraulics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-2
A.6 Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-3
A.7 Vacuum Degas Pump Assembly (Optional) . . . . . . . . . . . . . . . . . . . . .A-3
B • Installation
B.1 Facility Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .B-1
B.2 Installation Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .B-2
B.2.1 Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .B-2
B.2.2 Electronics Chassis Connections . . . . . . . . . . . . . . . . . . . . . . .B-4
B.2.3 DX-L AN Interface: 10B ASE-T Connections (Optional). . . . .B-6
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B.2.4 DX-LAN Interface: BNC Connections (Optional) . . . . . . . . . B-9
B.2.5 Waste Lines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-13
B.2.6 Eluent Inlet Line Connections. . . . . . . . . . . . . . . . . . . . . . . . B-14
B.2.7 Eluent Outlet Line Connections . . . . . . . . . . . . . . . . . . . . . . B-14
B.2.8 Piston Seal Wash Connections (Optional) . . . . . . . . . . . . . . B-15
B.2.9 Priming the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-17
B.3 Automatic SRS Power Control (Optional) . . . . . . . . . . . . . . . . . . . . B-20
C • Display Screens
C.1 Operational Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2
C.1.1 Menu of Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2
C.1.2 Main Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-3
Contents
C.2 Diagnostic Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-14
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C.1.3 Detail Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-5
C.1.4 Method Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-6
C.1.5 Method Extension Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-8
C.1.6 Degas Options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-9
C.1.7 Module Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-11
C.1.8 Pump Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-12
C.1.9 Time Function In. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-13
C.2.1 Diagnostic Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-14
C.2.2 Power-Up Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-15
C.2.3 Elapsed Time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-16
C.2.4 DSP Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-17
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GP50 Gradient Pump
C.2.5 DX-LAN Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-18
C.2.6 Keyboard Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-20
C.2.7 Diagnostic Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-21
C.2.8 Pressure Statistics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-23
C.2.9 DSP Code Version . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-24
C.3 Calibration Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-25
C.3.1 Calibration Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-25
C.3.2 Calibration Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-26
C.3.3 Leak Sensor Calibration and Status . . . . . . . . . . . . . . . . . . . .C-27
C.3.4 Degas Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-28
C.3.5 Flow Calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-29
C.3.6 Pressure Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-30
C.3.7 Degas Pump Calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-34
D • TTL and Relay Control
D.1 TTL and Relay Output Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . .D-2
D.2 TTL Input Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .D-3
D.2.1 TTL Input Signal Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .D-3
D.3 TTL and Relay Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .D-5
D.3.1 Example Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .D-6
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1.1 Overview
The GP50 Gradient Pump is an integral part of a Dione x chr omat ogr aphy system.
It is a microprocess or -based, dua l-pisto n, v ariable -speed, gr adient de li ver y system
designed to blend and pump mixtures of up to four different eluents at precisely
controlled flow rates. The pump can deliver the selected eluent composition
isocratically, or as a multistep linear or curved gradient. A Digital Signal
Processor (DSP) provides high speed control of pump flow and pressure.
The pump can operate as a stand-alone product or with other Dionex modules as
part of a complete chromatography system. It can also be used with non-Dionex
modules that meet interface requirements for software, TTL, or relay control.
The GP50 can be controlled locally, using the front panel keypad and display, or
from a remote host computer with a Dionex DX-LAN™ interface installed and
®
PeakNet
ava ilabl e from an y de vi ce capabl e of pro v iding compati ble TTL signa ls to contr ol
the pump.
The pump's two basic mode s of control, Dire ct control and Method control, e nable
it to operat e with or without reference to time-based events.
software installed on t he hos t comput er. Limited remote control is also
1 • Introduction
The GP50 is available in four versions. An optional vacuum degas pump is
available for all versions:
GP50 Gradient Pump V e r sio n With Degas Pump Without Degas Pump
Standard bore with PEEK
components
Standard bore with stainless steel
components
Microbore with PEEK components P/N 054045 P/N 054429
Microbore with stainless steel
components
Doc. 031377-03 7/01
P/N 054426 P/N 054427
P/N 054419 P/N 054420
P/N 054425 P/N 054424
1-1
GP50 Gradient Pump
1.2 About This Manual
Chapter 1
Introduction
Chapter 2
Description
Chapter 3
Operation and
Maintenance
Chapter 4
Troubleshooting
Chapter 5
Service
Appendix A
Specifications
Appendix B
Installation
Appendix C
Display Screens
Appendix D
TTL and Relay
Control
Gives an overview of the GP50 Gradient Pump, and
explains conventions used in this manual, including
safety-related information.
Describes the GP50 front panel controls, electronic and
mechanical components, and operating modes.
Provides an overview of GP50 opera tion, including how to
create, edit, and run methods from the GP50 front panel.
Lists routine preventive maintenance procedures.
Lists problems and presents step-by-step procedures for
how to isolate and eliminat e them.
Provides step-by-step instructions for routine service and
parts replacement procedures.
Lists the GP50 specifications and installation site
requirements.
Describes how to install the GP50.
Illustrates and describes all of the screens that can be
displayed on the GP50 front panel.
Describes relay and TTL input and output functions and
provides installation instructions
.
1-2
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1.2.1 Saf et y Me ss a ges and Note s
This manual contains warnings and precautionary statements that, when
properly followed, can prevent personal injury and/or damage to the
GP50. Safety messages appear in bold type and are accompanied by
icons, as shown below.
Indicates an imminently hazardous situation which, if not
avoided, will result in death or serious injury.
Indicates a potentially hazardous situation which, if not
avoided, could result in death or serious injury.
Indicates a potentially hazardous situation which, if not
avoided, may result in minor or moderate injury.
1 • Introduction
Doc. 031377-03 7/01
Indicates that the function or process of the instrument may be
impaired. Operation does not constitute a hazard.
Informational messages also appear throughout this manual. These are
labeled NOTE and are in bold type:
NOTE NOTES call attention to certain information. They alert
you to an unexpected result of an action, suggest how to
optimize instrument performance, etc.
1-3
GP50 Gradient Pump
1.2.2 Saf et y Label s
The TUV GS, C, US Mark safety label and the CE Mark label on the
GP50 indicate that the GP50 is in compliance with the following
standards: EN 61010-1:1993 (safety), CAN/CSA-C22.2 No. 1010.1-92
(safety), UL 3101-1/10.93 (safety), EN 50082-1:1992 (susceptibility),
and EN 55011:1991 (emissions).
The symbols below appear on the GP50, or on GP50 labels.
Alternating current
Protective conductor terminal
Power supply is on
Power supply is off
1-4
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2 • Description
The GP50 Gradient Pump consists of two units (see Figure 2-1). The upper unit
houses the electronics components and the lower unit houses the pump heads and
other mechanical pump assemblies.
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Pump
Heads
Figure 2-1. GP50 Enclosure
2-1
GP50 Gradient Pump
2.1 Front Control Panel
The control panel on the upper door of the pump enclosure contains a liquid
crystal display (LCD), a membrane keypad, and the actuator for the main power
switch (see Figure 2-2). The door opens to provide access to the electronics
chassis (see Section 2.2).
NOTE If no keypad buttons are pressed within a two-hour
period, the front panel backlight will automatically turn
off. To restore the backlight, press any button.
Screen Contrast
Information is displayed on the LCD, also called the
contrast, use the knurled knob in the recess below the keypad (see Figure 2-2).
Tilt Panel
To maximize visibility, the front control panel can be tilted to four different
positions. To tilt the panel, support the door at the left side (to prevent it from
opening) and lift firmly on the tab in the middle of the recess below the keypad
(see Figure 2 -2). Push on the tab t o return the panel to its vertical position.
. To adjust the screen
screen
2-2
Power Switches
The main powe r swit ch is on the bulkhead behind the uppe r door (see Figur e 2-1).
An actuator for the main power switch is on the outside of the front door, at the
lower left corner (see Figure 2-2).
The actuator functio ns only when the door is fully clos ed. Whe n the door is open,
press the main power switch on the bulkhead to turn the module off and on.
To prevent damage to the pump circuitry and components,
always wait at least 15 seconds after powering down before
turning on the power again.
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GP50 G radient Pump
100.0 %A
0.0 %B
0.0 %C
0.0 %D
Help Message
LOAD
COLUMN A
2000
2.00
LOCAL 2
10.27
METHOD
2•Description
PSI
m L /MIN
MIN
Off/O n
Prime Reset
Insert
Delete
Ma in P o wer
Sw itc h A c tu a to r
Hold/Run
Select
Select
Tab
(for opening
the door)
Help Menu
Figure 2-2. GP50 Display and Keypad Layout
2.1.1 Control Panel Keypad
Use the keypad to directly control pump operation, as well as to create
and modify programmed series of timed events, called
summary:
Press
_
_
To go from a menu to a screen, press the numeric button that
corresponds to the screen’s number on the menu, or move the cursor
to the desired screen name and press
to display a list of available screens.
Menu
Knob
(for adjusting
the contrast)
7
4
1
0
Enter
8
5
23
Tab
(for tilting
the panel)
9
6
Enter
methods
.
. In
Doc. 031377-03 7/01
Only fields shown in reverse video on a screen can be edited. Other
_
fields display information only.
To edit a value in a reverse video field, use the four directional arrow
_
buttons to move the cursor to the field. Use the numeric buttons to
2-3
GP50 Gradient Pump
enter variable values, or use the
choose from among predetermined options.
Select
∆
and
Select
buttons to
∇
Keypad
Buttons
To confirm the selected value, press
_
some screens or screen fields, pressing
NOTE A high-pitched beep sounds when you press a
button. When an error occurs, this beep is lower in
frequency. The beeps can be disabled from the
MODULE SET-UP
Function
Turns the pump motor off and on.
In Direct control (see Section 2.9), turnin g on the m otor cause s it to
pump isocratically using the displayed eluent percentages and flow rate.
In Method control (see Section 2.9.3), turning on the moto r causes it to
pump at the eluent percentages and flow rate for the elapsed time of the
selected method, or at the initial conditions (when the method clock is at
).
INIT
This button is used when priming the pump heads.
pump to run at maximum vo lume (2.5 mL/min, microb ore; 10.0 mL/min,
standard bore). If the pump motor is off when
pump automatically turns on. To exit priming and return to the normal
flow rate, press
See Section B.2.8 for detailed priming in str uctions.
Prime
screen (see Section C.1.7).
again or press
or an arrow button. In
Enter
is required.
Enter
Prime
is pressed, the
Prime
Off/On
to turn off the pump motor.
causes the
2-4
Inserts a new step into a method. This button functions only when the
cursor is in a
1. Move the cursor to the
added below the cursor position. Parameter values in the new step
are blank.
2. Fill in the time value and press
Note:
time value, the inserted step is not saved because it is incomplete.
3. Insert steps in any order. When you press
automatically reorganized in the correct chronological order.
TIME
field on the
If you move the cur sor out of the
Table 2-1. GP50 Front Panel Buttons
METHOD
field and press
TIME
METHOD
or
or a cursor arrow button.
Enter
TIME
Enter
extension screen.
. The new step is
Insert
field before entering a
, they will be
Doc. 031377 -03 7/01
2•Description
Keypad
Buttons
Function
Removes the value from the current entry field. To restore the previous
value, move the cursor from the field before entering a new value.
Pressing
screen “blanks” the step parameter value. Moving the cursor to another
field does not restore the previo us v alue; ins tead, the step remains blank,
indicating no change from the previous step.
To delete an entire method step:
1. Position the cursor in the method’s time field and press
2. Press
Turns the method clock off (
only when the pump is under Method control (see Section 2.9.3).
When the method clock is in Hold, pressing
at either the initial step of a new method or, if resuming an interrupted
method, at the time at which the clock was put in Hold.
When the method clock is in Run, pressing
this “holds” the method and freezes the current conditions.
Delete
time is removed and the help line prompts you to press
again to delete the step.
parameters, press any button except
when the cursor is in a step entry field o n the
Delete
again. Or, to restore the original time and step
.
Delete
) and on
Hold
(Run)
Hold/Run
Hold/Run
METHOD
. The
Delete
Delete
. This button functions
starts the clock
stops the clock;
Doc. 031377-03 7/01
Changes the method clock time to
specified by the method to occur. This button functions only when the
GP50 is under Method control (see Section 2.9.3).
If the method is running, it continues running. If the method is in Hold,
the method clock executes the initial conditions and holds.
When the cursor is positioned at a field with predetermined parameters,
these buttons cycle through the options. In fields with predetermined
numeric values, pressing
pressing
Select
Enter
button increases (or decreases) the value continuously. Press
or a cursor arrow button to confirm the selected value.
Table 2-1. GP50 Front Panel Buttons (Continued)
∇ decreases the value by one unit. Holding down a
Select
Select
, causing the initial conditions
INIT
∆
increases the value by one unit;
2-5
GP50 Gradient Pump
Keypad
Buttons
Function
The four cursor buttons move the cursor, in the direction of the arrow, to
the next entry field. If there is no changeable field in that direction, the
cursor moves diagonally or remains in its current location.
In most cases, after entering a new value in an entry field, pressing an
arrow button saves and/or executes the change. The exceptions are the
field, the
METHOD SAVE TO
command, the
Displays a help screen with information pertaining to the current entry
field.
Displays one of three menus, depending on the current screen:
_
From an operational screen, pressing
SCREENS
_
From a diagnostic screen, pressing
DIAGNOSTIC MENU
From a calibration screen, pressing
_
CALIBRATION MENU
Enters numeric values into the current entry field. The numeric buttons
are 0 through 9 and the decimal.
DIAGNOSTIC TESTS
.
METHOD RUN
.
.
screen, and all menu screens.
Menu
Menu
Menu
any calibration
field,
displays the
displays the
displays the
MENU of
2-6
From a menu screen, press ing a nu meri c b utt on open s t he co rres pon ding
screen.
Saves and/or executes changes made in entry fields. If a menu screen is
displayed, pressing
Table 2-1. GP50 Front Panel Buttons (Continued)
opens the highlighed screen.
Enter
Doc. 031377 -03 7/01
2.1.2 Display Screens
When the pump has successfully powered-up and passed all diagnostic
tests, the
the
power-up, the
screen. See Section C.2.7 if this occurs.
POWER-UP
screen (see Figure 2-4). If one of the diagnostic tests fails at
MAIN
DIAGNOSTIC TEST
2•Description
screen (see Figure 2-3) displays briefly, followed by
screen displays instead of the
MAIN
NOTE
Help Message
The
selecting the screen from the
(see Section C.2.1).
GP50 GRADIENT PUMP
Figure 2-3. Power-Up Screen
100.0 %A
0.0 %B
0.0 %C
0.0 %D
Help Message
POWER-UP
screen can be viewed at any time by
PUM P H EA D VO LUM E 100 uL
MODULEWARE REV n.nn
B IO S R E V n.n n
LOAD
2000
COLUMN A
2.00
10.27
LOCAL 2
METHOD
DIAGNOSTIC MENU
PSI
mL /MIN
MIN
Doc. 031377-03 7/01
Figure 2-4. Main Screen
The
allow viewing from a d istance. Use the
parameters, such as the flow rate and the percentages of eluents to run.
T o access oth er GP50 screens, pre ss the
of SCREENS
screen displays status information in enlarged char acters to
MAIN
screen to select operating
MAIN
button to displ ay th e
Menu
(see Figure 2-5).
MENU
2-7
GP50 Gradient Pump
There are two ways to select a screen from a menu:
Press the numeric button on the front panel keypad that corresponds
_
to the screen number on the menu. For example, p re ss 3 to di splay th e
METHOD
Move the cursor to the field containing the screen number and press
_
Enter
See Appendix C for a description of each screen.
screen.
.
MENU of SCREENS
1
M AIN S CR E EN
2
DETAIL SCREEN
3
METHOD
4
DEGAS OPTIONS
Help Message
Figure 2-5. Menu of Screens
2.2 Electronics Chassis
The electronics chassis is located behind the upper door of the pump enclosure.
The chassis includes se v eral ele ctronics car ds (printed ci rcuit boards) that are used
to control the pump. Connectors on the cards also allow communication between
the pump and other Dionex chromatography modules. Figure 2-6 shows the
electronics components with the upper door open. To open the door, pull on the
tab located to the right of the main power actuator (see Figure 2-2).
Do not remove any of the electronics cards from the pump.
There are no user-serviceable components on the cards. If
servicing is required, it must be performed by qualified
personnel and appropriate electrostatic discharge (ESD)
handling procedures must be followed.
5
MOD ULE SET-UP
PUMP OPTIONS
6
7
TIME FUNCTION IN
8
DIAGNOSTIC MENU
2-8
Ne retirez aucune des cartes électroniques de la pompe. Auc un
des composants sur les cartes ne peut être réparé par
l'utilisateur. Toute réparation doit être effectuée par un
personnel qualifié utilisant des procédures correctes de
décharge électrostatique.
Doc. 031377 -03 7/01
2•Description
PWR SPY
L
C
L
E
A
K
GP50/IP25-DSP130W
DIST
MOTOR
SLOT 2SLOT 1
BLANK
D
L
C
C
O
M
M
P6
I
L
S
C
T
A
1
I
3
R
P
I
N
P7
D
I
S
T
1
2
P
I
N
P8
SLOT 3
BLANK
SLOT 4
LAN-000K
RLY-1
OUT
RLY-2
OUT
+
TTL-1
-
OUT
+
TTL-2
-
OUT
+
TTL-1
-
IN
+
TTL-2
-
IN
+
TTL-3
-
IN
+
TTL-4
-
IN
POWER SUPPLY
GREEN - OK
RED - FAULT
SLOT 5
CPU/RLY
F
R
O
N
T
P
A
N
E
L
Figure 2-6. GP50 Electronics Chassis
(Located behind pump door)
LC LEAK
The leak control cable from the LC10 Chromatography Organizer or
LC20 Chromatography Enclosure, connects t o the
LC LEAK
connector in
slot 1. When a leak occurs i n the LC10 or LC2 0 it is re ported to t he GP50.
Doc. 031377-03 7/01
NOTE The LC25 Chromatography Oven and LC30
Chromatography Oven do not connect to the GP50
LEAK
connector. These ovens contain internal leak
LC
control electronics.
LC COMM
The LC30 Chromatography Oven's RJ-11 serial cable connects to the
connector in slot 1. When connected, the LC30 can be remotely
COMM
controlled by the PeakNet workstation.
LC
2-9
GP50 Gradient Pump
LC AIR
The cable from the ai r soleno id v alv es in t he LC10, LC20, L C25, or LC3 0
chromatography module conne cts to the
connected, the GP50 can electrically actuate the solenoid valves that
control the position of the injection valve and the optional column
switching valve in the chromatography module.
LC AIR
connector in slot 1. When
To select the valve positions, go to either the
Section C.1.2) or the
TTL/RELAY
METHOD
screen (see Section C.1.4).
MAIN
screen (see
A strip of eight relay and TTL connectors is located in slot 4. These
connectors interface with Dionex and non-Dionex modules for relay and
TTL control of the pump. Appendix D describes the relay and TTL
functions and the connections between the GP50 and other modules.
CPU
Control Moduleware for the pump resides on the CPU/Relay cards.
The CPU logic and Relay I/O cards occupy slot 5. The Relay I/O card
rides piggybac k o n t he CPU card and exten ds over the f ro nt of slot 4. The
card is short enough to allow the optional DX-LAN pump interface card
(P/N 044195) to mount behi nd it in slot 4. A 60- pin ri bbon cab le lin ks the
CPU logic to the display and keypad. The logic monitors the internal
power supply outputs, and reports the status on the multicolored LED at
the bottom of slot 4.
Green indicates normal operation.
_
Red indicates a power fault. The GP50 will enter its diagnostic state
_
and inhibit all other cont rols unti l the f ault is corr ected . If this occurs,
turn off the power for a few seconds and then turn it back on.
2-10
Doc. 031377 -03 7/01
2.3 Mechanical Chassis
The mechanical chassis is housed in a pull-out drawer located behind the lower
door of the pump enclosure. The front of the chassis contains the components
described in Section2.4. Other mechanical assemblies are located inside the
chassis drawer. The drawer should be pulled out only for service procedures. For
routine operation, push in the drawer and tighten the lock located on the lower
right corner of the chassis.
Observe the warning label on the inside of the lower door. The
arrows on the label indicate moving mechanical parts that
present pinch hazards when the pump is on and the
mechanical drawer is open. Do not operate the pump with the
mechanical chassis drawer pulled out.
Respectez l'étiquette d'avertissement apposée à l'intérieur de
la porte inférieure. Les flèches sur l'étiquette indiquent des
pièces mécaniques mobiles qui posent un danger de
pincement lorsque le GP50 est sous tension et le tiroir
mécanique est ouvert. N'ut ilisez jamais le GP50 avec le tiroir
du châssis mécanique ouvert.
2•Description
Doc. 031377-03 7/01
2-11
GP50 Gradient Pump
2.4 Mechanical Components
Figure 2-7 shows the mechanical componen ts located behi nd the lo wer door of the
enclosure.
Pressure
Transducer
Pressure
Transducer
Waste Valve
Pr im ing
Block
Pump
Heads
2-12
Eluent
Manifold
Proportioning
Figure 2-7. GP50 Mechanical Components
Doc. 031377 -03 7/01
2.4.1 Pump Eluent Manifold
Eluent lines A through D are routed through the rear of the GP50 to the
vacuum de g as pump ( if ins ta ll ed) (see Sec ti on 2.5) and then to the rear of
the proportioning valve. If the vacuum degas pump is not installed, the
eluent lines are connected directly to the proportioning valve. The proper
proportion of eluent exits the front of the valve and is directed to the
priming block. Figure 2-8 shows the eluent flow path through the system.
2•Description
Eluent Reservoirs
VAC Chamber IN
VAC Chamber OUT
ABCD
Pump H eads
OUT
D
Proportioning Valve/
A
C
Manifold Assembly
B
Pressure
Transducer
Figure 2-8. Eluent Flow Schematic
Mixer To Column
Rinse Ports
Priming Block
Doc. 031377-03 7/01
2-13
GP50 Gradient Pump
2.4.2 Pump Heads
There are two types of pump heads: standard bore and microbore. The
table below summarizes the features and operating conditions for each
type.
Pump Head
Type
Standard
Bore
Microbore 25µL Isocratic and
*Flow rates are adjustable in increments of 0.01 mL/min.
NOTE Although there is some overlap in flow rates
Piston
Volume
100µL Isocratic 0.04–10.0 35 MPa
between the two pump configurations, continuous
operation of the microbore pump at flow rates
above 2.0 mL/min will decrease seal and pump life.
For the best extended operation at 2.0 mL/min or
above, use a standard bore pump. To achieve
optimum performance at flow rates below
0.4 mL/min, use a microbore pump.
Pump
Operation
Gradient 0.4–10.0 35 MPa
Gradient
Flow Rate
(mL/min)*
0.0–2.50 35 MPa
Maximum
Operating
Pressure
(5000 psi)
(5000 psi)
(5000 psi)
2.4.3 Pump Mixers
A pump mixer ensures complet e mixi ng of the proportioned eluents prior
to injection. The mixer is installed between the pump outlet and the
injection valve. Three types of mixers are available:
_
_
_
2-14
In standard bore PEEK pumps, a GM-5 Mixer (P/N 054044) is used.
In microbore PEEK pumps, a GM-4 Mixer (P/N 049136) is used.
In stainless ste el pumps , a Sta inles s Ste el Mi x er (P /N 0 54043) is use d.
Doc. 031377 -03 7/01
2.4.4 Pump Priming Block
The priming block “tee” directs the flow of eluent fr om the manifo ld i nto
the pump heads. The priming block is also used for rapid removal of air
from the system.
Refer to Section B. 2.8 for instructions on priming the pump heads.
2.4.5 Pressure Transducer
From the priming block, the liquid stream is directed to the inlet check
valves on the pump heads, through the pump heads, and finally through
the outlet check valves to the pressure transducer.
Flow from the outlet check valves on the pump heads is combined in the
pressure transducer. The pressure transducer measures the system
pressure at this point. The interactive constant-flow/constant-p ressure
control program on the DSP precisely controls the pump motor speed to
assure flow rate accuracy.
A waste line exits the bottom of the pressure transducer. Opening the
valve on the pressure transducer diverts flow to the waste line and relieves
system pressure, forcing air out of the system.
2•Description
Doc. 031377-03 7/01
Flow output from the pressure transducer is directed out of the pump
module, through the gradient mixer, and on to the rest of the
chromato graphy system (inj ection valve, column, detector).
See Section B.2.6 for eluent outlet line connections.
2-15
GP50 Gradient Pump
2.5 Vacuum Degas Pump Assembly (Optional)
The Dionex vacuum degas pump provides continuous on-line vacuum degassing
of up to four eluents. The assembly, which must be installed at the factory,
consists of :
A 4-channel degas chamber (with degas membranes) with 17 mL internal
_
capacity p er channel
A dual-stage diaphragm vacuum pump
_
A solenoid valve
_
An on-board vacuum sensor
_
The electronics required to operate the vacuum pump
_
Fittings, tubing, and other accessories
_
By default, the de gas pump tur ns on f or 2 minu tes when th e GP50 power is turned
on. Thereafter, the pump turns on for 30 seconds at 10-minute intervals. The
DEGAS OPTIONS
Section C.1.6). You can check the vacuum chamber pressure from the
STATUS
screen (see Section C.3.4).
screen allows you to change the cycle time and duration (see
DEGAS
NOTE All components of the vacuum degas assembly are made
of inert materials or corrosion-resistant materials.
However, Dionex recommends thoroughly flushing any
chemicals out of the tubing with deionized water after
each use to avoid crystallization in the membrane pores.
2.6 Piston Seal Wash (Optional)
When using highly concentrated buffer solutions, Dionex recommends
continuously rinsing the piston seal with a piston seal wash. Rinsing removes salt
crystals that may abrade the piston, thereby causing the seal to wear out
prematurely and allow leaks.
Dionex offers a continuous seal wash kit (P/N 059187) for the GP50. For
installation instructions, see Section B.2.8 or the instructions included with the
kit.
2-16
Doc. 031377 -03 7/01
2.7 Eluent Reservoirs
NOTE Dionex strongly recommends degassing all eluents and
storing them in reservoirs pressurized with helium. This
helps prevent bubbles (resulting from eluent outgassing)
from forming in the el uent proportionin g valves, pump
heads, and the detector cell.
Degassed eluents and pressurized reservoirs are
especially important when combining aqueous and nonaqueous components (e.g., water and methanol).
Pressurizable reservoirs allow eluents to be stored under
a specific atmosphere.
The following pressurizable reservoirs are available from Dionex:
1-liter glass reservoirs with shatterproof plastic coating (P/N 044126)
_
2-liter glass reservoirs with shatterproof plastic coating (P/N 044127)
_
1-liter plastic reservoirs (P/N 044128)
_
2•Description
2-liter plastic reservoirs (P/N 044129)
_
Do not use the 2-liter plastic reser voir (P/N 044129) for off-line
vacuum degassing of eluents. The reservoir was not designed
for this purpose.
N'utilisez pas le réservoir en plastique de 2 litres (N /P 044129)
pour le dégazage à vide hors ligne d'éluants. Le réservoir n'a
pas été conçu à cette fin.
Refer to the
Pressurizable Reservoir Installation Instructions
(Document No.
034581) for installation details.
EO1 Eluent Organizer (Optional)
The Dionex EO1 Eluent Organizer (P/ N 044 125) holds eluent reservoirs in a liner
that contains spills and leaks. The EO1 can also be used to pressurize reservoirs.
Up to two optional E01 Eluent Organizers can be places on top of the system
Doc. 031377-03 7/01
2-17
GP50 Gradient Pump
enclosure. Each organizer can accommodate one or two reservoirs, depending on
the type of reservoir used (see the table below).
No. of
Reservoirs
2 1-Liter glass or plastic 2 L
2 2-Liter plastic 4 L
1 2-Liter glass 2 L
Description Total Volume
2.8 Rear Panel
The rear panel contains the main power receptacle and fuses. It also includes a
DX-LAN connector for interfacing the GP50 with the PeakNet workstation. The
rear panel is illustrated in Figure B-1.
2.9 Functional Description
2.9.1 Operating and Control Modes
The operating mode determines
commands:
_
In Local mode, the GP50 receives commands from the front control
panel buttons and screens.
in Liters
how
the GP50 receives operating
2-18
In Remote mode, PeakNet 5 software sends commands from the host
_
computer via the DX-LAN int erfac e. Limited o perating changes fr om
the front panel are allowed.
_
In Locked Remote mode, PeakNet 5 or PeakNet 6 software sends
commands from the host computer via the DX-LAN interface. All
operating changes from the f ront pane l are disab led. When PeakNet 6
software is controlling the GP50, the pump is always in Locked
Remote mode.
The control mode determines
_
In Direct control, the GP50 exe cutes commands immediately.
when
operating commands are executed.
Because there is no time-ba sed program, th e method clock i s not used
and the
Hold/Run
and
buttons do not operate.
Reset
Doc. 031377 -03 7/01
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