Galileo SEMI
Semi-automatic microtome
User Manual
Manufacturer:
Diapath S.p.A.
Via Savoldini,71
24057 Martinengo (BG) Italy
Tel. (+39)0363.986.411
Fax (+39)0363.948.000
www.diapath.com
info@diapath.com
2
MANUAL INFORMATION
It is forbidden partly or in full to modify this manual in absence of any explicit authorization.
Information contained in this manual are subject to changes without prior notice.
Diapath S.p.A. has done as much as possible to assure the accuracy of this manual.
However, in case something seems to be wrong or not clear, please contact Diapath S.p.A or the distributor.
Diapath adopts continuously a development policy and reserves the right to make changes and
improvements to any product described in this manual without prior notice. In no case Diapath S.p.A. will be
considered responsible for possible losses of data or earnings or for any other datum.
Manual revision: Rev. 02
Edition date: 30th May 2016
Inspired to:
Galileo Galilei (Pisa, 15th February 1564 – Arcetri, 8th January 1642) was an Italian physician,
philosopher, astronomer and matematician, considered the founder of modern science. His
name is associated to important theories in dynamics and astronomy, such as telescope
perfectioning, which bring him to make important astronomic observations and to introduce the
scientific method. Fundamental were his role in the astronomic revolution and his support to the
heliocentric system and to the Copernican theory. His importance for the re-valuation of the
science in general and of physics in particular is connected to discoveries that he made through
several experiments such as relativity principle, the discover of Jupiter 4 moons, exactly called
Galilean satellites, inertia principle and the discovery that speed fall is the same for all bodies
independently from material or mass.
3
PREFACE
Destination of use
Galileo is a semi-automatic rotary microtome for creating thin sections of specimens embedded in paraffin.
The instrument may be operated by trained and qualified staff only.
It is possible to set up all process parameters, such as: sectioning and trimming thickness and the retraction
value of the specimen holder before the stroke phase.
The reading of this manual and the maintenance are essential to use the equipment avoiding any situation
of danger.
The operators shall be adequately trained in its use and must respect all safety rules and its use limits.
Instrument maintenance, due to trouble or malfunction, shall be performed by trained and authorized
Diapath S.p.A. staff.
The instrument complies with CISPR 11 Class A rules: in case of domestic use, it may cause radio
interferences. In this case, it is necessary a measurement and mitigation of such interference.
Before installing the instrument, perform an analysis of electromagnetic interferences.
Do not use the device near sources of strong electromagnetic radiations, such as radiofrequency waves
generators. In this case the radiations may interfere with the instrument normal functioning.
Instrument has been projected according to the legislative decree 332/00 of 08th September 2000
“Accomplishment of 98/79/CE directive of 27th October 1998 concerning in vitro diagnostic medical devices”
respecting electromagnetic compatibility rules:
• 2007/47/CEE Directive 93/42/EEC concerning the updating of Directives 98/79/EEC on medical devices
and medical devices in vitro, which came into force on 21/03/2010 by Legislative Decree 25/2010.
• Legislative Decree 27
amending Directive 95/16/EC relating to lifts.
• ISO9001(*): quality management system requirements.
• ISO13485(*): quality management system requirements for medical devices. Requirements for
regulatory purposes and specific requirements of the application of ISO 9001(*)
• UNI CEI EN ISO 14971(*): application of risks management to medical devices.
• UNI EN 591(*): Instructions for use of in vitro diagnostic instruments for professional use.
• UNI CEI EN 980(*): Graphical symbols for use in the labeling of medical devices.
• EN 61326-1(*): Electrical equipment for measurement, control and laboratory use - EMC requirements
Part 1: General requirements.
• EN 61326-2-6(*): Electrical equipment for measurement, control and laboratory use - EMC
requirements. Part 2-6: Particular requirements - In vitro diagnostic medical devices (IVD).
th
January 2010, n. 17 implementing Directive 2006/42/EC on machinery and
4
• EN 61000-3-2(*): Electromagnetic compatibility (EMC) Part 3-2: Limits - Limits for harmonic current
emissions. (equipment with input current <= 16A per phase).
• EN 61000-3-3(*)+ A1(*)+ A2(*): Electromagnetic compatibility (EMC) Part 3-3: Limits - Limitation of
voltage fluctuations and of flicker in low voltage supply systems for equipment with rated current <=
16A and not subject to conditional connection.
• EN 61010-1(*): Safety requirements for electrical equipment for measurement, control and laboratory
use. Part 1: General requirements.
• EN 61010-2-101(*): Safety requirements for electrical equipment for measurement, control and
laboratory use. Part 2-101: Particular requirements for medical equipment for in-vitro diagnostics.
• The LED light according to standard EN62471 and classified in 1 Risk Group Category.
(*)
In the applicable edition
5
QUALITY CONTROL
Diapath S.p.A. adopts quality management system ISO 9001(*) and ISO 13485(*) to project, make and
improve the technical-scientific solution for the customer in medical and diagnostic in vitro systems, assuring
high quality services with the help of an highly qualified and motivated staff to contribute to the
improvement of Diapath S.p.A. products.
Diapath S.p.A. constantly looks for customer’s satisfaction, tries to perceive their expectations and to treat
the customer without any discrimination. Diapath S.p.A. assures the application of normative requirements.
Quality assurance of data furnished by Diapath S.p.A. is obtained through these activities:
• Use only reference material
• Execution of proofs of the whole production
• Correlation of different results
The internal quality control foresees to monitor the most difficult passages of an analytic process so as to
verify their stability, to assure that quality parameters estimated during the validation or check of the
method are valid also for real instruments and that they won’t get worse in the course of the time.
Diapath S.p.A. tests its own products in accordance with some internal procedures, to reduce possibility of
anomalies during the operation.
To identify and to find out its own instruments, Diapath S.p.A. uses some internal procedures in which we
can find production recording, assembling recording, tests recording, serial number or batch code and
configuration recording.
(*)
In the applicable edition
6
SAFETY INFORMATION
Mentioned agreement and graphic symbols
Danger - The danger instructions signal situations that might cause death or serious
lesions.
Caution - The caution instructions signal situations that might cause physical
lesions to people or damages to things.
Biohazard - The biohazard instructions signal situations that might cause the
contamination of people or parts involved in the process.
Cutting and shearing - The cutting and shearing instructions signal situations that
might cause lesions to the operator.
Crush hazard - The crush hazard instructions signal situations where body parts might
be crushed by moving parts.
Instructions of danger
Never disassemble, modify or repair the instrument or one of its parts without written
authorization by Diapath S.p.A.
Do not use any kind of different power supply from the mentioned ones.
For use at voltages higher than 125V in the North American market must be used electricity
supply cables with NEMA 6-15P plug, C13 plug connector and STJ cable with nominal
capacity 13A 250V.
7
Instructions of caution
Do not place objects that are not involved in the use of the instrument.
The use of appropriated individual protection devices is recommended (For instance:
gloves).
Instructions of biohazard
Keep attention to the possible biological hazard of the parts involved in the process.
Special individual safety measures shall be used by operators, in accordance with the
standards for safety in the laboratories and product safety data sheets in relation to current
rules.
Do not place objects that are not involved in the use of the instrument.
Safety training
All the operators must be trained to use the microtome safely.
After such training, the operators must have understood that:
• The microtome must be connected to a voltage source in accordance with electrical data label.
• The use of the microtome differently from Diapath instructions might compromise its functioning
and the safety of the operator
Compliance with safety rules
All regulations related to safety, local codes and instructions that appear in the manual or on the equipment
must be carefully observed to ensure personal safety and to prevent damage to the microtome. If the
equipment is incorrectly used, the protection provided might be compromised.
8
In vitro diagnostic medical
European Community approval
Foot switch
connection
Remote control
Conformity with
TUV
certification
DEFINITION OF ADOPTED SYMBOLS
device
Manufacturer
Built in
Danger
Disposal
mark
See the user manual
Electrical shocks risk
CAN/CSA-C22.2 No
61010-1 requirements,
2nd edition
RFID antenna
9
STORAGE AND HANDLING
For a correct conservation and functioning of the microtome, all the instructions provided in this manual
concerning the maintenance and the installation shall be respected. Also the showed below environmental
requirements shall be respected.
The instrument can work with 80% relative humidity conditions for temperatures up to 31°C with a linear
decrease up to 50% at the temperature of 40°C. Voltage variation can’t exceed +/-10% the nominal value.
The instrument is planned only for internal use and up to 2000 m o.s. altitude.
Storage and transportation temperature range: da +5°C a +40°C
Storage humidity: 80%
Working temperature range: Da +5°C a +40°C
Due to size and weight (more than 30 Kg), the handling of the microtome must be
performed by several people and with suitable lifting means.
10
SUMMARY
MANUAL INFORMATION .................................................................................................................. 3
Inspired to: ...................................................................................................................................... 3
PREFACE ........................................................................................................................................... 4
Destination of use .................................................................................................................................... 4
QUALITY CONTROL .......................................................................................................................... 6
SAFETY INFORMATION .................................................................................................................... 7
Mentioned agreement and graphic symbols .................................................................................... 7
Instructions of danger .............................................................................................................................. 7
Instructions of caution ............................................................................................................................. 8
Instructions of biohazard ......................................................................................................................... 8
DEFINITION OF ADOPTED SYMBOLS ............................................................................................... 9
STORAGE AND HANDLING ............................................................................................................. 10
INSTRUMENT PLACEMENT AND INSTALLATION ........................................................................... 13
1.1 Unpacking .................................................................................................................................. 13
1.2 Setting up before installation .................................................................................................... 13
1.3 Startup ....................................................................................................................................... 13
INSTRUMENT SETTING .................................................................................................................. 15
2.1 Before the use .................................................................................................................................. 15
2.1.1 Controls before the use .................................................................................................... 15
2.1.2 License activation ............................................................................................................. 15
PARTS OF THE INSTRUMENT ......................................................................................................... 16
3.1 Directional specimen holder with clamping system ......................................................................... 17
3.2 Knife holder ...................................................................................................................................... 18
3.3 Emergency stop switch .................................................................................................................... 19
3.4 Optionals .......................................................................................................................................... 19
3.4.1 Clamp for mega cassettes ................................................................................................ 19
3.4.2 Rapid release kit ............................................................................................................... 19
3.5 Control panel .................................................................................................................................... 20
CONTROL PANEL FUNCTIONALITY ................................................................................................ 23
4.1 Main screen ...................................................................................................................................... 23
4.2 License activation ............................................................................................................................. 24
4.3 Limited license renewal .................................................................................................................... 26
4.4 Log card creation ............................................................................................................................. 29
4.5 Positioning memory ......................................................................................................................... 31
4.6 Alarm messages ............................................................................................................................... 33
CONTROL INTERFACE FUNCTIONALITY ........................................................................................ 35
5.1 Main screen ...................................................................................................................................... 35
5.2 Configuration menu ......................................................................................................................... 36
5.2.1 Setting date and hour ...................................................................................................... 37
5.2.2 Configuring language ....................................................................................................... 38
5.2.3 Configuring retraction ...................................................................................................... 39
5.2.4 Displaying information screen .......................................................................................... 40
PERFORMANCES AND LIMITATIONS ............................................................................................. 41
SPECIFICS AND INSTALLATION..................................................................................................... 42
CLEANING AND MAINTENANCE ..................................................................................................... 44
8.1 LCD display cleaning ........................................................................................................................ 44
8.2 External surfaces .............................................................................................................................. 44
8.3 Instrument cleaning frequency ........................................................................................................ 44
9 45
SCHEDULED MAINTENANCE ........................................................................................................... 45
9.1 Checking the directional specimen holder ....................................................................................... 45
9.2 Cleaning the knife holder ................................................................................................................. 45
11
9.3 Cleaning the clamp .......................................................................................................................... 45
9.4 Cleaning the section waste tray ....................................................................................................... 45
9.5 Replace fuses ................................................................................................................................... 46
TROUBLESHOOTING ...................................................................................................................... 47
WEEE DIRECTIVE ........................................................................................................................... 49
11.1 WEEE directives ............................................................................................................................. 49
11.2 Warranty certificate ........................................................................................................................ 50
12
1
INSTRUMENT PLACEMENT AND INSTALLATION
1.1 Unpacking
Unpack instrument parts taking care to not damage them. The instrument has been tested before the
shipping.
Make sure about any serious damages to the packaging and to the instrument due to the transport. In case
of damages, please contact Diapath or the local distributor.
Due to size and weight (more than 30 Kg), the handling of the microtome must be performed
by several people and with suitable lifting means.
1.2 Setting up before installation
Before the installation, please verify that electrical supply sources are set according to the data supplied by
the manufacturer (see instrument specifics on Chapter 7).
Place the instrument on a plane surface, stable, vibration free and able to support at least the double weight
of the product (see instrument specifics on Chapter 7).
Power cable should be easily accessible. Verify that the clearance between the instrument rear side and a
possible wall is at least 20 cm.
The instrument must be installed with a side distance from possible walls of at least one meter.
1.3 Startup
ATTENTION
Instrument placement and installation must be performed by Diapath S.p.A. trained and authorized
staff.
Perform the following installation steps:
• Place the instrument on a flat surface
• Assemble the activation handwheel
• Install, if required, all supplied accessories
• Connect the power cable of the instrument. Be sure to do this when the instrument is off
13
ATTENTION
Instrument must be connected to a grounded power socket!
DO NOT use extension cables
ATTENTION
The power cable plug disconnects the instrument from the electric system. Verify that the power
cable plug is always easily accessible.
Connecting Remote Control - Symbol
The remote control connector is on the rear side of the instrument,
identified by this symbol. Insert the connector and screw the threaded
ring to lock off.
14
2
INSTRUMENT SETTING
2.1 Before the use
2.1.1 Controls before the use
It is a good practice to perform the following operations, before starting up the microtome:
• Verify that the power cable is in good conditions.
• Verify that the specimen holder can move freely up and down
• Verify that the knife and specimen holder are securely clamped to the instrument
2.1.2 License activation
Before using Galileo microtome is necessary to activate the license, which can be:
• Limited license: the microtome is activated for a period of time, after which the coarse feed
system is disabled. To reactivate the functioning, it is necessary to renew the license.
• Unlimited license: the microtome is activated unlimitedly, without an expiration date.
The license (and the following renewals in case of limited license) is activated by RFID system. To activate
or renew the license, is necessary a valid RFID card and follow instructions described in section 4.2 and 4.3.
15
Pic.1 -
Parts of the microtome
3
PARTS OF THE INSTRUMENT
Control panel
Separate control
panel
Emergency stop
button
Section waste
tray
Knife holder with
knife guard
Directional specimen holder fixture
with clamping system
16
Setscrews
Cassette release lever
Pic. 2
– Adjustable head
3.1 Directional specimen holder with clamping system
Head release lever for
orientation
To perform the orientation of the specimen: unlock the cassette tension lever, use setscrews for an
appropriate orientation of the specimen, lock the lever.
To mount the cassette in the clamp: push the lever and mount the specimen
ATTENTION
Specimen orientation must be performed only during the advancing phase. If performed during the
retraction phase it may cause errors in the cutting thickness and damages to the specimen!
17
Pic. 3 - Knife holder
3.2 Knife holder
1-Clamping lever for the
lateral displacement
2-Knife guard with ejector
3-Clamping lever for
the blade
4-Clamping lever for
knife holder base
To move the knife holder toward the specimen, release lever 4, mount it and lock the lever 4.
To insert the blade, release the lever 3, push in the blade and lock lever 3. Cover always the cutting edge
with the knife guard when the instrument isn’t used. To remove the blade, use the ejector after releasing
lever 3.
To move the blade right and left, release lever 1, mount the blade in the desired position and lock lever 1.
ATTENTION
Clamp always the specimen before clamping the blade.
Lock the handwheel and cover the knife-edge with the knife guard before performing any manipulation
of the knife!
ATTENTION
Be careful especially with handling microtome blades: they are extremely sharp and can cause serious
lesions!
18
1-Base
2-Slide
3.3 Emergency stop switch
The emergency stop switch is on the left side of the microtome. The cutting motor stops immediately when
the emergency stop switch is pressed. The specimen holder can be moved manually, allowing the user to
operate for the safety.
To restore microtome functionality, switch off the emergency stop switch and wait that the specimen holder
has completed the positioning at the beginning of the horizontal feed (position 0).
3.4 Optionals
3.4.1 Clamp for mega cassettes
The clamp for mega cassettes allows to section mega cassettes with maximum length of 67mm.
3.4.2 Rapid release kit
Rapid release kit, composed of a base (1) to be applied on the directional specimen holder, and a slide (2) to
be applied to the clamps, allows to quickly replace clamps.
To replace a clamp, release the upper screw, push the clamp toward left and insert a different clamp. Finally
lock the upper screw previously released.
19
Pic. 4 -
Control panel
3.5 Control panel
The control panel displays the current operating mode, possible alarms and information. It displays also
information about current cutting session:
• Position of the specimen holder referring to horizontal feed;
• Number of completed sections
• Cutting/Trimming mode with its thickness in micron.
Control panel has 5 buttons:
Activation of RFID system and management of the license. In case of limited license, press this
button to display remaining days to expiration; if pressed twice in 5 seconds, it enables the RFID
system for license renewal or for log card writing (see section 4.3 and 4.4). In case of unlimited
license, press this button to enable the RFID reader for log card writing (see section 4.4)
resetting of the set sections number counter
Management of positioning memory. Pressing the button the present position is stored;
pressing the button the specimen holder moves to the last stored position (see sect.4.5)
Switching on/off the light for slide visual inspection
20
Pic. 5
- Control interface
3.6 Control interface
The control interface allows to set up microtome operating parameters. During operating are displayed the
thickness values in micron during the Cutting/Trimming mode.
Control interface has the following buttons:
• LOCK/UNLOCK. When the microtome is in LOCKED mode, the movement is
automatically locked
• POSITION: horizontal movement of the specimen holder. If pressed, they allow to position
the specimen holder in the desired position.
• TRIM/SECT: if pressed, it allows to select between:
o Cutting
o Trimming
NOTE: mode ON is displayed with white text, mode OFF with grey text.
21
• If pressed, they allow to set up the section thickness. If the Trimming mode is ON is
modified the Trimming thickness, otherwise the Cutting thickness.
• MENU: press it to gain access to microtome configuration menu (see sect.5.2).
Use the following buttons to surf the menu:
o To choose the entry of the menu
o To confirm (and save) the selected entry of the menu
o To exit from the current menu without saving
22
Pic.6 - Main screen
4
CONTROL PANEL FUNCTIONALITY
4.1 Main screen
After switching on the microtome using the power switch on the rear side, the control panel shows the main
screen, similar to the one in the pic.5. The screen is divided in four sections:
1. Date, hour and system status: system status is represented by the icon displayed on the upper
left angle.
Thur 13 Sep 2012
11:45
Position
325
Section No
15
Cutting
3.0
• LOCKED: system locked and safe
• UNLOCKED: the system can be moved manually by the handle. It is possible to
section
2. Position: it displays in which horizontal feed position the specimen holder is. When switching on
the microtome, the specimen holder moves to the horizontal feed start position (Position 0). The
horizontal feed has a maximum length of 30000, when reached an alarm message is displayed.
3. Section number: it is the completed cuttings number counter. It can be cleared by pressing the
button.
4. Cutting/Trimming: it displays the mode in operation and the section thickness in micron.
23
t
he reader
Pic
. 7 -
Expiry license pop
-
up
Pic. 8 -
RFID card message screen
4.2 License activation
The activation is performed by RFID system: it is necessary to have the appropriated card.
There are two licenses:
• Limited license: the microtome is activated for a period of time, after which the coarse feed
system is disabled. To reactivate the functioning, it is necessary to renew the license.
• Unlimited license: the microtome is activated unlimitedly, without an expiration date.
To activate the license (limited or unlimited) press the button. On display appears a pop up with
the number of days remaining before expiration:
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Days to expiry: 5
15
Cutting
3.0
Pressing again the button within 5 seconds the RFID system is activated. On the display appears the
following message:
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Place the card next to
15
Cutting
3.0
24
Cutting
Pic. 9 - Tracking card screen
Pic.
10 -
Successful new license starting up screen
Bring the card close to the RFID reader, situated under the lit window. When the card reader tracks the
card, appears the following message:
Keep the card close to the reader until appears the message confirming license activation:
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Please wait…
15
3.0
Thur 13 Sep 2012
11:45
Posizione
325
Numero sezioni
Starting up complete
15
Cutting
3.0
25
Pic.
11 –
Pop-up of remaining days to license expiration
Possible error messages are:
NON-VALID CARD
The used card is not valid or already used. The license isn’t activated
NON-VALID CARD
TIMEOUT
TIMEOUT
and RFID system is deactivated
RFID reader didn’t track any card within 5 seconds from the
activation. The license isn’t activated and RFID system is
deactivated.
4.3 Limited license renewal
At license expiration, the coarse feed system is deactivated. To reactivate it, provide a card for license
renewal and proceed as follows.
1. Pressing the button: on display appears a pop up with days remaining before expiry:
ATTENTION
If number of remaining days is less than 45, the pop up appears, as note, each time the microtome is
switched on.
If the number of remaining days is less than 30 the pop up alarm is accompanied by an acoustic alarm.
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Days to expiry: 5
15
Cutting
3.0
26
t
he reader
Cutting
Pic
. 12 - RFID card message screen
Pic.13
-
Card tracking message screen
2. Pressing again the button within 5 seconds the RFID system is activated. On display appears
the following message:
3. Bring the card close to the RFID reader, placed under the back lit panel. When the card reader
tracks the card, appears the following message:
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Place the card next to
15
Cutting
3.0
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Please wait…
15
3.0
27
Pic. 14
-
Successful new license starting up screen
4. Keep the card close to the reader until appears the message confirming successful procedure:
Thur 13 Sep 2012
11:45
Position
325
15
3.0
Possible error messages are:
NON-VALID CARD
The used card is not valid or already used. The license isn’t renewed
NON-VALID CARD
TIMEOUT
TIMEOUT
and RFID system is deactivated
RFID reader didn’t track any card within 5 seconds from the
activation. The license isn’t renewed and RFID system is
deactivated.
Numero sezioni
Starting up complete
Cutting
28
t
he reader
Pic
. 15 - RFID card message screen
Pic
. 16 - Tracking card message screen
4.4 Log card creation
The Galileo microtome RFID system allows to create a card containing the log of the operations carried out
by the RFID system itself.
Provide oneself with the appropriated card and proceed as follows:
1. Press the button (once if unlimited, twice if limited license) until appears the pop up with the
following message:
2. Bring the card close to the RFID reader, placed under the back lit panel. When the card reader
tracks the card, appears the following message:
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Place the card next to
15
Cutting
3.0
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Please wait…
15
Cutting
3.0
29
Complete log codification screen
3. Keep the card close to the reader until appears the message confirming log creation:
Possible error messages are:
NON-VALID CARD
TIMEOUT
No . RFID reader didn’t track any card within 5 seconds from the
activation. The log has not been created and the RFID system is
deactivated.
TIMEOUT
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Log writing complete
15
Cutting
3.0
Pic. 17 -
NON-VALID CARD
The used card is not valid or already used. The log hasn’t been
created and RFID system is deactivated.
30
Pic. 18
-
Memorization of specimen holder position screen
4.5 Positioning memory
The and buttons allow to manage positioning memory in order to have the possibility to bring
the specimen holder to the stored position, to easily return to first cuttings position.
Pressing the button the present position, on which the operator wants to return, is stored. A popup
signals the successful position memorization:
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Saved position
15
Cutting
3.0
31
Pic. 19
- Specimen holder under positioning screen
Pic. 20
-
Specimen holder stored position screen
To return to the stored position, press the button
When the specimen holder reaches the stored position, a confirmation message appears:
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Positioning in progress
15
Cutting
3.0
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
Positioning complete
15
Cutting
3.0
32
MOTOR FEED
REMOTE CONTROL
Pic. 21
-
End running alarm screen
4.6 Alarm messages
If a functioning anomaly occurs, an alarm message appears in the pop-up:
Alarm messages are the following (identification of each alarm by a 3 numbers code):
ALARM 802
FEED END
ALARM 251
ALARM 150
Thur 13 Sep 2012
11:45
Position
325
Numero sezioni
ALARM 802
15
FEED END
Cutting
3.0
ALARM 802 FEED END: The horizontal feed end has been reached
(Position = 30000): the specimen holder can’t move anymore. The
alarm message disappears if the holder returns (pressing the
on the control interface or button on the control panel to
return to stored position).
ALARM 251 MOTOR FEED: Bad functioning of automatic feed
system occurs.
ALARM 150 REMOTE CONTROL: A problem with connection to
control interface occurs.
33
CONNECTION
ALARM 151
ALARM 801
IN EMERGENCY
ALARM 750
EXPIRED LICENSE
ALARM 151 CONNECTION: a problem on communication protocol
between electronic boards occurs.
ALARM 801 IN EMERGENCY: this message appears when
emergency button is pressed. Unlocking it the message disappears.
ALARM 750 EXPIRED LICENSE: the license of the microtome is
expired and feed system is deactivated. To reactivate instrument
functioning, provide yourself a RFID activation card and proceed as
in sect. 4.3.
34
Pic
. 22 - Main screen
5
CONTROL INTERFACE FUNCTIONALITY
5.1 Main screen
After switching the on microtome, using the rear power switch, the control interface displays the main
screen, similar to the one in the picture above.
The main screen displays the thickness of the section in micron during Cutting and Trimming mode. The
Cutting
3.0
Trimming
10.0
mode ON is displayed in white color, the mode OFF in grey color. To switch between modes press the
button.
Press the buttons to modify the value of the thickness for the mode ON.
Cutting
3.0
Trimming
10.0
Cutting
3.0
Trimming
10.0
35
Retraction
Date & hour
Pic. 23 - Configuration menu screen
5.2 Configuration menu
Language
Information
Press the button to enter configuration menu (see Pic. 23).
ATTENTION
It is possible to enter configuration menu only if system is in LOCKED mode
Use the following buttons to surf the menu:
• To scroll the menu
• To confirm the choice
• To go back to the previous screen. This button is the “ESCAPE” function: if pressed, in any
configuration menu, it allows to go back to the previous screen without saving changed
settings
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Date
Pic. 24 -
Date & hour menu screen
5.2.1 Setting date and hour
Select the Date & hour entry in the configuration menu to enter the related menu:
Date & hour
13/ 9/2012
Hour
11:45
The two triangular symbols show which field between data or hour can be changed.
Press the buttons to set up the value in the activated field.
Press the button to move to the following field.
The fields are activated in the following sequence:
DAY -> MONTH -> YEAR -> HOUR -> MINUTES
Press the button to go back to previous menu without changes.
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Language
setting screen
5.2.2 Configuring language
Select the Language entry in the configuration menu to enter the related menu:
Italiano
English
Español
Français
Press the buttons to choose the language and press the button to confirm.
Deutsch
Pic. 25 - Language
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Off
Pic
. 26 - Configuration retraction screen
Pic. 27
- Retraction value setting screen
5.2.3 Configuring retraction
Select the Retraction entry in the configuration menu to enter the related menu:
Retraction
On
Selecting the entry Off, the specimen holder don’t retract before vertical stroke.
Select the entry On to enter the screen in which to set up the retraction value in micron.
Retraction
Value [micron]
5
Press the buttons to set up the desired value and then confirm with the button.
39
Firmware version:
Pic. 28
-
Information display screen
5.2.4 Displaying information screen
Select Information entry in the configuration menu to enter the screen with microtome serial number (16
digits) and firmware version of each electronic sheet (C= control board, L= local board, R= remote board).
Information
1.0.0
Serial number:
1234567890abcde
www.diapath.com – info@diapath.com
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6
PERFORMANCES AND LIMITATIONS
Galileo is a rotary microtome projected to create histological sections of specimens embedded in paraffin.
Users might use the equipment according to the limits described in this manual and following moral and
good-sense procedures.
The warranty is valid only if all performances and use limitations described in this manual are respected.
Diapath S.p.A. refuses any contractual and non-contractual responsibility for damages caused by any
mistakes done working in the non-compliance of instructions given by the manufacturer.
Diapath S.p.A. refuses any responsibility if the microtome is not connected to electrical systems according to
the local regulations.
Any other use not indicated in this manual is to consider as inappropriate and dangerous. We suggest to
protect the microtome from an improper use that could be dangerous.
Environmental electromagnetic conditions should be evaluated before instrument use.
Do not use the instrument near high electromagnetic radiations sources: they could interfere with
instrument working.
It is operator’s responsibility ensure to keep an environment electromagnetically compatible with instrument
features
.
41
Dimensions (
WxDxH
) 400x675x350 mm
Weight
40 Kg
Supply
voltage
115-230 V~
±10%
F
requency
50-60Hz
Maximum power consumption
200VA
Power
cable
type
F(Schuko)
– type
B - type
I
Fuses
F3.15 AL 250V
Overvoltage category
2
Altitude
Up to 2000 m
Relative humidity
80%
Operative temperature range
From
5°C to 40°C
Pollution degree
2
Noise level
Max 60 dBA
Certifica
tions
CE -
IVD - cTUVus
7
SPECIFICS AND INSTALLATION
Semi-automatic microtome Galileo SEMI
Technical features
Electrical features
Environmental features
Horizontal and plane surface able to support
the double of the product weight. Keep away
from any direct heating sources or humidity.
Place the instrument in a way that the main
power supply plug can be easily removed.
The instrument isn’t suitable for external use
General features
42
Cutting thickness setting range
0.5 - 100 micron
0.5 - 5 micron
in 0.
5 micron increments
Trimming section thickness setting range
1 -
600 micron
5 -
30 micron in 5 micron increments
Object feed
30 mm +
- 1 mm, feed motion via step motor
Vertical stroke
70 mm
Maximum specimen size
55 x 50
mm (68 x 50 with clamp for mega
-
cassettes)
Horizontal specimen orientation
8°
Vertical specimen orientation
8°
Specimen rotation
360°
Specimen retraction
5 -
100 micron in 5 micron increments, can be
Technical features
100 - 200 micron in 20 micron increments
200 - 600 micron in 50 micron increments
5 - 20 micron in 1 micron increments
20 - 30 micron in 2 micron increments
30 - 60 micron in 5 micron increments
60 - 100 micron in 10 micron increments
30 - 100 micron in 10 micron increments
turned off
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8
CLEANING AND MAINTENANCE
ATTENTION
Before each action indicated below, switch the instrument off.
ATTENTION
We suggest using suitable individual protection media (gloves, masks, etc. according to the situations)
to perform the cleaning safely.
8.1 LCD display cleaning
To clean the display, use a screen detergent (in accordance with the instructions defined by the detergent
manufacturer).
8.2 External surfaces
If necessary, clean the surfaces with a cloth dampened with water or water and soap, then wipe with a
dry cloth.
ATTENTION
Do NOT use any solvents to clean the exterior painted parts.
8.3 Instrument cleaning frequency
We recommend you to perform the complete cleaning of the instrument at the end of each working day.
44
9
SCHEDULED MAINTENANCE
This chapter describes cleaning and inspections to be performed on the entire system. Maintenance plans
need daily, monthly and six-month checks.
Clean each part of the instrument at least once a week for a correct and lasting instrument use.
ATTENTION
Before cleaning, perform decontamination as specimens could be potentially infectious. Follow all
laboratory regulation, disinfect section waste tray and the parts in contact with the operator: handle;
remote control. We suggest using suitable individual protection media (gloves, masks, etc. according to
the situations) to perform in safety these cleaning.
ATTENTION
Dispose contaminated material according to the laboratory regulations, handling waste as potentially
contaminated waste.
9.1 Checking the directional specimen holder
Once a month check that the directional specimen holder isn’t obstructed, otherwise clean it and lubricate
orientation hemispheres.
9.2 Cleaning the knife holder
We recommend you to perform the cleaning of the knife holder at the end of each working day. Be careful
to remove all paraffin and section residues.
ATTENTION
Before cleaning, be sure that microtome is switched off and blade/knife is removed.
9.3 Cleaning the clamp
We recommend to perform the cleaning of the clamp after each use. Be careful to remove all paraffin
residues.
9.4 Cleaning the section waste tray
We recommend to perform the complete cleaning of the section waste tray at the end of each working day.
Be careful to remove all residues.
45
Pic. 2
9
Pic.
31
Pic. 30
9.5 Replace fuses
To replace fuses:
• Switch the unit off and unplug it (Pic. 29)
• Open fuse housing with light pressure on point shown in Pic.30
• Remove fuses (see Pic.
interrupted; in that case replace the fuse with one
with the same rating, positioning it in place
(For ratings see section 11.0)
• Connect the power cable and switch the unit on
See section “Troubleshooting” in case of functioning problems.
If problem persists, contact Diapath or the local distributor.
ATTENTION
We suggest a preventive maintenance operation at least once in a year.
Pic.
31) and check that the filament is not
46
Possible cause
Corrective action
The
power
cable is disconnected
Check the connect of the power cable
Electrical system protections are intervened
Microtome protections are intervened
No voltage
Contact the engineering service or qualified
Power key has not been pressed
Press the key on the control panel
Possible cause
Corrective action
The end of the residual path has been
Move the specimen holder
to position 0
Stepp
er
motor is locked
Contact
Diapath or the local distributor
Possible cause
Corrective action
Blade,
knife
holder or specimen holder not
Check clamping system of each mechanism
Check
that the cassette is
securely
blocked
Laterally displace the knife holder; insert a
Possible cause
Corrective
action
The blade is exhausted
Use a new blade
The s
pecimen is too warm
C
ool the specimen before cutting
Possible cause
Corrective action
Check all screw and clamp connections on
Possible cause
Corrective action
10
TROUBLESHOOTING
The instrument does not turning on
The specimen holder does not move
reached
Cutting too thin/thick sections
clamped properly
The specimen is not clamped properly
The blade is dull
Clearance angle of the blade too small
Set again the protection
Check and if necessary replace power fuses
staff for the verification of electric systems
in the cassette clamp
new blade
Change using clearance angle settings
Cutting deformation
Noises during cutting
The clearance angle is too wide
Insufficient clamping of specimen clamp or
knife holder
Too great of a cutting force was applied
If problems persist or are not described in the previous chart, contact Diapath S.p.A. or the local distributor.
High blade consumption
Change clearance angle until you have found
the optimum angle
the specimen holder system and the knife
holder
Adjust the cutting speed and/or section
thickness
47
Description
Article code
COMPLETE BODY GALILEO
SEMI
S
DSMA9008
KNIFE HOLDER GALILEO MICROTOME
SDSPL9001
N
SECTIONS HOLDER CLAMP
SDSMA9015
SECTION WASTE TRAY GALILEO
SDSMS0096
WIRED REMOTE CONTROL
L
GALILEO
SEMI
S
DSMS9033
COMPLETE ELECTRONIC SHEET GALILEO UL
SDSMA9059
COMPLETE ELECTRONIC
SHEET GALILEO
SDSMA9006
N
MOTOR ROTATION ASSEMBLY
SDSMA9012
SPARE PARTS LIST
48
11
WEEE DIRECTIVE
11.1 WEEE directives
Before uninstalling the instrument, perform its decontamination, according to current laboratory standards.
Before transporting the instrument, insert it into a suitable packaging to minimize the risk of contact with the
parts of the instrument.
In compliance with EU Directive 2002/96/EC or waste electrical and electronic equipment (RAEE), this
electrical product must not be disposed of as unsorted municipal waste. Please dispose of this product by
returning it to the point of sale or to your local collection or recycling point.
The directive gives measures to prevent production of electronic and electrical equipment waste and their
re-employment, recycling and other way of collection to reduce disposal waste volume.
The Directive RAEE/WEEE (electronic and electrical equipment waste) assists the different collecting of
technological waste coming from the disuse of electric equipment malfunctioned, broken or not repairable.
This directive RAEE (electronic and electrical equipment waste) introduces the concept of “empty to return”
for the electronic and electrical waste, or rather the old equipment withdrawal when you buy a new one.
Diapath S.p.A. is an ecor’it member, global system of WEEE management (electronic and electrical
equipment waste) home and professional in the Italian territory.
To guarantee an efficient and effective system of separated collection of WEEE for the Italian territory, in
accordance with the law device, ecor’it uses suppliers that are the better of existent reality concerning
disposable technique, withdrawal and recycle.
In the European territory the RAEE responsible (electronic and electrical equipment waste) is the
manufacturer.
The European directive 2002/96/CE of European Parliament and council of 27th January 2003 on “electrical
and electronic equipment waste WEEE” defines manufacturer who:
i. Produce or sell electronic and electrical equipment under its brand;
ii. Sell under its brand equipment produced by other suppliers. The reseller is not considered as
“the manufacturer” if the equipment has manufacturer’s trademark according to the point I;
iii. Imports or exports electronic or electrical equipment to a member state concerning a
professional activity.
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11.2 Warranty certificate
50
Diapath S.p.A.
Via Savoldini,71
24057 Martinengo (BG) Italy
Tel. (+39)0363.986.411
Fax (+39)0363.948.000
www.diapath.com
info@diapath.com
51
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