Decagon Devices AquaLab 4TE, AquaLab DUO, AquaLab 4TEV Operator's Manual

AquaLab
Water Activity Meter
Operator’s Manual
For Series 4TE, 4TEV, DUO
Version 7
Decagon Devices, Inc.
2365 NE Hopkins Court
Pullman WA 99163
(509)332-2756
fax: (509)332-5158
www.aqualab.com
support@decagon.com
sales@decagon.com
Trademarks
AquaLab is a registered trademark of
Decagon Devices, Inc.
©2008-2012 Decagon Devices, Inc.
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Contents
1. Introduction .................................1
About this Manual ....................................1
Customer Support .....................................1
Warranty ...................................................2
Seller’s Liability ..........................................2
2. About AquaLab ...........................4
AquaLab Model and Options ....................4
AquaLab 4 Instrument Speci cations ........4
AquaLab 4 DUO Speci cations .................5
How AquaLab Works ................................6
AquaLab and Temperature ........................6
Chilled Mirror Dewpoint Limitations .......8
3. Water Activity  eory ...................9
Moisture Content ......................................9
Water Activity............................................9
Water Potential ........................................12
Sorption Isotherms ..................................13
4. Getting Started ........................... 15
Components of your AquaLab ................. 15
Choosing a Location ............................... 15
Preparing AquaLab for Operation ............16
5. Menus ......................................... 18
Measurement Tab ....................................18
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Con guration Tab ................................... 19
Admin Settings ........................................25
Data Tab .................................................30
6. Cleaning and Maintenance ........32
Cleaning the Block and Sensors ...............33
Cleaning a Series 4TEV: .......................34
Cleaning Procedure: ...............................34
Veri cation of Calibration .......................36
7. Ve r i cation and Calibration ........37
Water Activity Veri cation .......................37
Veri cation of Calibration .......................39
8. Sample Preparation ....................46
Preparing the Sample ...............................46
Samples Needing Special Preparation ......47
Slow Water-Emitting Samples ..................48
Volatile Samples .......................................49
Low Water Activity ..................................50
Samples Not at Room Temperature .........50
9. Taking a Reading ....................... 52
Measurement Steps ..................................52
How AquaLab Takes Readings ................52
10. Duo Operation (Optional) ....... 55
Obtaining Product Isotherm Models .......56
Loading and Organizing Product Models 56
Moisture Content Adjustment ...............60
Restore Original Moisture Content Model
Setting s .................................................... 6 4
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How to Delete Models .............................66
11. Computer Interface ...................68
AquaLink RG ..........................................68
Using Windows Hyperterminal .............68
12. Troubleshooting .......................70
13. Support and Repair ..................83
Repair Costs ............................................84
Loaner Service .........................................84
14. Further Reading .......................85
Water Activity  eory & Measurement ...85
Food Safety and Microbiology .................89
Appendix A ................................... 111
Preparing Salt Solution .......................... 111
Appendix B ................................... 113
Temperature Correction ........................ 113
Appendix C ................................... 114
AquaLab Veri cation Standards ............ 114
Declaration of Conformity ........... 119
Certi cate of Traceability .............120
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AquaLab
1. Introduction
1
1. INTRODUCTION
Welcome to Decagon’s AquaLab Series 4, 4TE, 4TEV, and DUO, the industry standard for measuring water activity (aw). AquaLab is the quickest, most accurate, and most reliable instrument available for measuring water activity. Whether you are researching or work­ing on the production line, AquaLab will suit your needs. It is easy to use and provides accurate and timely results.
About this Manual
Included in this manual are instructions for setting up your AquaLab, verifying the calibration of the instrument, preparing samples, and maintaining and caring for your instrument. Please read these in­structions before operating AquaLab to ensure that the instrument performs to its full potential.
Customer Support
If you ever need assistance with your AquaLab, or if you just have questions or feedback, there are several ways to contact us:
NOTE: If you purchased your AquaLab through a distributor, please contact them for assistance.
E-mail support@aqualab.com
Please include your name, contact information, instrument serial number(s), and a description of your problem or question.
sales@aqualab.com
Please include your name, address, phone number, the items you wish to order and a purchase order number. Credit card numbers should always be called in.
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1. Introduction
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Phone
1-800-755-2751 (USA and Canada Only) 1-509-332-2756 International
Our Customer Support and Sales Representatives are available Monday thru Friday.
Fax
1-509-332-5158
Warranty
AquaLab has a 30-day satisfaction guarantee and a three-year war­ranty on parts and labor. Your warranty is automatically validated upon receipt of the instrument. We will contact you within the  rst 90 days of your purchase to see how the AquaLab is working for you.
Seller’s Liability
Seller warrants new equipment of its own manufacture against de­fective workmanship and materials for a period of three years from date of receipt of equipment (the results of ordinary wear and tear, neglect, misuse, accident and excessive deterioration due to corro­sion from any cause are not to be considered a defect); but Seller’s liability for defective parts shall in no event exceed the furnishing of replacement parts Freight On Board the factory where originally manufactured. Material and equipment covered hereby which is not manufactured by Seller shall be covered only by the warranty of its manufacturer. Seller shall not be liable to Buyer for loss, damage or injuries to persons (including death), or to property or things of whatsoever kind (including, but not without limitation, loss of anticipated pro ts), occasioned by or arising out of the installation,
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1. Introduction
3
operation, use, misuse, nonuse, repair, or replacement of said ma­terial and equipment, or out of the use of any method or process for which the same may be employed.  e use of this equipment constitutes Buyer’s acceptance of the terms set forth in this warranty.  ere are no understandings, representations, or warranties of any kind, express, implied, statutory or otherwise (including, but with­out limitation, the implied warranties of merchantability and  tness for a particular purpose), not expressly set forth herein.
AquaLab
2. About AquaLab
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2. About AquaLab
AquaLab is the fastest and most accurate instrument for measuring water activity, giving readings in  ve minutes or less. Its readings are reliable, providing ±0.003 aw accuracy.  e instrument is easy to clean and checking calibration is simple.
AquaLab Model and Options
Series 4: Uses chilled-mirror dewpoint sensor, but lacks tempera-
ture control features of premium models.
Series 4TE: User-selectable internal temperature control model, uses thermoelectric (Peltier) components to maintain internal tem­perature.
Series 4TEV: Uses both a chilled-mirror dewpoint sensor and a ca­pacitance sensor for measuring non-volatile and volatile substances, respectively. Either sensor is easily selected using the instrument’s menu system.
Series 4TE DUO: Uses chilled-mirror dewpoint and programmed models obtained from isotherm data to give the user both water ac­tivity and moisture content simultaneously in  ve minutes or less.
AquaLab 4 Instrument Speci cations
Water Activity Range: 0.050 to 1.000 a
w
Water Activity Accuracy: ±0.003 (4TE Dew Point Mode) Water Activity Accuracy: ±0.015 (4TEV Capacitance Mode) Water Activity Resolution: 0.0001
AquaLab
2. About AquaLab
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Read Time1: ≤5 min. Sample Temperature Range: 15 to 50° C Sample Temperature Accuracy: ±0.2° C Sample Temperature Resolution: 0.01° C Sample Dish Capacity: 15ml Full Operating Environment: 5 to 50° C 20 to 80% Humidity Case Dimensions: 26.7 x 17.8 x 12.7cm Weight: 3.1 Kg Case Material: Lustran 433 (ABS) with  re retardant Display: 64 x 128 Graphical Data Communications: RS232A Serial, 9600 to 115200 baud Power: 110 to 220 VAC, 50/60Hz Warranty: 3 year parts and labor
1
On samples with no signi cant impedance to vapor loss
AquaLab 4 DUO Speci cations
Moisture Content Repeatability: 0.02% Accuracy to Moisture Content Ref.: 0.1% to 0.5%
AquaLab and Water Activity
Water activity (aw) is a measurement of the energy status of the water in a system. It indicates how tightly water is “bound”, structurally or chemically, within a substance. Water activity is the relative hu­midity of air in equilibrium with a sample in a sealed measurement chamber.  e concept of water activity is of particular importance in determining product quality and safety. Water activity in uences color, odor,  avor, texture and shelf-life of many products. It predicts safety and stability with respect to microbial growth, chemical and biochemical reaction rates, and physical properties. For a more de­tailed description of water activity as it pertains to products, please refer to Chapter 3 of this manual, titled “ Water Activity  eory”.
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2. About AquaLab
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How AquaLab Works
AquaLab uses the chilled-mirror dewpoint technique to measure the water activity of a sample. In an instrument that uses the dewpoint technique, the sample is equilibrated with the head-space of a sealed chamber that contains a mirror and a means of detecting condensa­tion on the mirror. At equilibrium, the relative humidity of the air in the chamber is the same as the water activity of the sample. In the AquaLab, the mirror temperature is precisely controlled by a thermoelectric (Peltier) cooler. Detection of the exact point at which condensation  rst appears on the mirror is observed with a photo­electric cell. A beam of light is directed onto the mirror and re ected into a photo detector cell.  e photo detector senses the change in re ectance when condensation occurs on the mirror. A thermo­couple attached to the mirror then records the temperature at which condensation occurs. AquaLab then signals you by beeping and dis­plays the  nal water activity and temperature.
In addition to the technique described above, AquaLab uses an in­ternal fan that circulates the air within the sample chamber to reduce equilibrium time. Since both dewpoint and sample surface tempera­tures are simultaneously measured, the need for complete thermal equilibrium is eliminated, which reduces measurement times to less than  ve minutes.
AquaLab and Temperature
Samples not read at room temperature during the read cycle will equilibrate with the AquaLab’s temperature before the water activity is displayed. Large temperature di erences will cause longer reading times, since a complete and accurate reading will not be made until the sample and the instrument are within 2°C of each other.
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2. About AquaLab
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 ere are several advantages in having a temperature-controlled wa­ter activity meter. A few major reasons are:
1. Research purposes. Temperature control can be used to study the e ects of temperature on the water activity
of a sample, make a comparison of the water activity of di erent samples independent of temperature, and conduct accelerated shelf-life studies or other water activity studies where temperature control is critical.  ere are many shelf-life, packaging, and isotherm studies in which tempera­ture control would be very bene cial.
2. To comply with government or internal regulations for speci c products.  ough the water activity of most products varies by less than ± 0.002 per °C, some regulations require measurement at a spe­ci c temperature.  e most common speci cation is 25°C, though 20°C is sometimes indicated.
3. To minimize extreme ambient temperature  uctuations. If the environmental and AquaLab temperatures  uctuate by as much as ± 5°C daily, water activity readings will vary by ± 0.01 aw. Temperature control eliminates variations due to changes in ambient conditions.
Series 4TE/4TEV/4TE-DUO
 e AquaLab Series 4TE models have thermoelectric components installed to allow the instrument to maintain a set chamber tem­perature.  e temperature is set using the con guration menu of any of the Series 4 models.
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2. About AquaLab
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Chilled Mirror Dewpoint Limitations
AquaLab’s limitation is its ability to accurately measure samples with high concentrations (typically >1%) of certain volatiles such as etha­nol or propylene glycol, which can condense on the surface of the chilled mirror.  e extent of the e ect is determined by how readily the material volatilizes, which is both concentration- and matrix­dependent.  erefore, even if your sample contains materials that could volatilize, it may still be possible to make accurate readings using the chilled mirror dewpoint sensor.
AquaLab Series 4TEV which incorporates both a chilled mirror sen­sor and a capacitance sensor for measuring volatile substances is Decagon’s solution for products containing volatile materials. If you are unsure if you need the TEV model, please call and discuss your product with a Decagon Representative. Refer to Chapter 8’s section titled ”Volatile Samples” or call Decagon for more details.
AquaLab
3. Water Activity  eory
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3. Water Activity  eory
Water is a major component of foods, pharmaceuticals, and cosmet­ics. Water in uences the texture, appearance, taste and spoilage of these products.  ere are two basic types of water analysis: moisture content and water activity.
Moisture Content
 e meaning of the term moisture content is familiar to most peo­ple. It implies a quantitative analysis to determine the total amount of water present in a sample. Primary methods for determining moisture content are loss on drying and Karl Fisher titration, but secondary methods such as infrared and NMR are also used. Mois­ture content determination is essential in meeting product nutri­tional labeling regulations, specifying recipes and monitoring pro­cesses. However, moisture content alone is not a reliable indicator for predicting microbial responses and chemical reactions in materi­als.  e limitations of moisture content measurement are attributed to di erences in the intensity with which water associates with other components.
Water Activity
Water activity is a measure of the energy status of the water in a system, and thus is a far better indicator of perishability than water content. Figure 1 shows how the relative activity of microorganisms, lipids and enzymes relate to water activity. While other factors, such as nutrient availability and temperature, can a ect the relationships, water activity is the best single measure of how water a ects these processes.
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3. Water Activity  eory
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Fig. 1: Water Activity Diagram—adapted from Labuza
Water activity of a system is measured by equilibrating the liquid phase water in the sample with the vapor phase water in the head­space and measuring the relative humidity of the head-space. In the AquaLab, a sample is placed in a sample cup which is sealed inside a sample chamber. Inside the sample chamber is a fan, a dew point sensor, a temperature sensor, and an infrared thermometer.  e dewpoint sensor measures the dewpoint temperature of the air in the chamber, and the infrared thermometer measures the sample temperature. From these measurements, the relative humidity of the head-space is computed as the ratio of dewpoint temperature saturation vapor pressure to saturation vapor pressure at the sample temperature. When the water activity of the sample and the rela­tive humidity of the air are in equilibrium, the measurement of the head-space humidity gives the water activity of the sample.  e pur­pose of the fan is to speed equilibrium and to control the boundary layer conductance of the dewpoint sensor.
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In addition to equilibrium between the liquid phase water in the sample and the vapor phase, the internal equilibrium of the sample is important. If a system is not at internal equilibrium, one might measure a steady vapor pressure (over the period of measurement) which is not the true water activity of the system. An example of this might be a baked good or a multi-component food. Initially out of the oven, a baked good is not at internal equilibrium; the outer surface is at a lower water activity than the center of the baked good. One must wait a period of time in order for the water to migrate and the system to come to internal equilibrium. It is important to remember the restriction of the de nition of water activity to equi­librium.
Temperature E ects
Temperature plays a critical role in water activity determination. Most critical is the measurement of the di erence between sample and dewpoint temperature. If this temperature di erence were in er­ror by 1°C, an error of up to 0.06 aw could result. In order for water activity measurements to be accurate to 0.001, temperature di er­ence measurements need to be accurate to 0.017°C. AquaLab’s in­frared thermometer measures the di erence in temperature between the sample and the block. It is carefully calibrated to minimize tem­perature errors, but achieving 0.017°C accuracy is di cult when temperature di erences are large. Best accuracy is therefore obtained when the sample is near chamber temperature.
Another e ect of temperature on water activity occurs when sam­ples are near saturation. A sample that is close to 1.0 aw and is only slightly warmer than the sensor block will condense water within the block.  is will cause errors in the measurement, and in subsequent measurements until the condensation disappears. A sample at 0.75 aw needs to be approximately 4°C above the chamber temperature to
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cause condensation.  e AquaLab warns the user if a sample is more than 4°C above the chamber temperature, but for high water activity samples the operator needs to be aware that condensation can occur if a sample that is warmer than the block is put in the AquaLab.
Water Potential
Some additional information may be useful for understanding what water activity is and why it is such a useful measure of moisture status in products. Water activity is closely related to a thermody­namic property called the water potential, or chemical potential (μ) of water, which is the change in Gibbs free energy (∆G) when water concentration changes. Equilibrium occurs in a system when (µ) is the same everywhere in the system. Equilibrium between the liquid and the vapor phases implies that () is the same in both phases. It is this fact that allows us to measure the water potential of the vapor phase and use that to determine the water potential of the liquid phase. Gradients in (µ) are driving forces for moisture movement.  us, in an isothermal system, water tends to move from regions of high water potential (high aw) to regions of low water potential (low aw). Water content is not a driving force for water movement, and therefore can not be used to predict the direction of water move­ment, except in homogeneous materials.
Factors In Determining Water Activity
 e water activity of the water in a system is in uenced by factors that e ect the binding of water.  ey include osmotic, matric, and pressure e ects. Typically water activity is measured at atmospheric pressure, so only the osmotic and matric e ects are important.
Osmotic E ects
Osmotic e ects are well known from biology and physical chemis­try. Water is diluted when a solute is added. If this diluted water is
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3. Water Activity  eory
13
separated from pure water by a semi-permeable membrane, water tends to move from the pure water side through the membrane to the side with the added solute. If su cient pressure is applied to the solute-water mixture to just stop the  ow, this pressure is a measure of the osmotic potential of the solution. Addition of one mole of an ideal solute to a kilogram of water produces an osmotic pressure of
22.4 atm.  is lowers the water activity of the solution from 1.0 to
0.98 aw. For a given amount of solute, increasing the water content of the systems dilutes the solute, decreasing the osmotic pressure, and increasing the water activity. Since microbial cells are high con­centrations of solute surrounded by semi-permeable membranes, the osmotic e ect on the free energy of the water is important for determining microbial water relations and therefore their activity.
Matric E ects
 e sample matrix a ects water activity by physically binding water within its structure through adhesive and cohesive forces that hold water in pores and capillaries, and to particle surfaces. If cellulose or protein were added to water, the energy status of the water would be reduced. Work would need to be done to extract the water from this matrix.  is reduction in energy status of the water is not osmotic, because the cellu­lose or protein concentrations are far too low to produce any signi cant dilution of water.  e reduction in energy is the result of direct physical binding of water to the cellulose or protein matrix by hydrogen bonding and van der Waal forces. At higher water activity levels, capillary forces and surface tension can also play a role.
Sorption Isotherms
Relating Water Activity to Water Content
Changes in water content a ect both the osmotic and matric bind­ing of water in a product.  us a relationship exists between the water activity and water content of a product.  is relationship is
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3. Water Activity  eory
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called the sorption isotherm, and is unique for each product. Besides being unique to each product, the isotherm changes depending on whether it was obtained by drying or wetting the sample.  ese fac­tors need to be kept in mind if one tries to use water content to infer the stability or safety of a product. Typically, large safety margins are built into water content speci cations to allow for these uncertainties.
While the sorption isotherm is often used to infer water activity from water content, one could easily go the other direction and use the water activity to infer the water content.  is is particularly attrac­tive because water activity is much more quickly measured than wa­ter content.  is method gives particularly good precision in the center of the isotherm. In order to infer water content from water activity, one needs an isotherm for the particular product. Decagon sells an Isotherm Generator called the AquaSorp IG or you can also have Decagon run the isotherm for a fee.
For example, if one were using the AquaLab to monitor the water content of dried potato  akes, one would measure the water activity and water content of potato  akes dried to varying degrees using the standard drying process for those  akes. An isotherm would be con­structed using those data, and the water content would be inferred using the measured water activity of samples and that isotherm. We have an upgrade available to Series 4TE users that would allow you to determine moisture content and water activity simultaneously.  is instrument is called the Series 4TE DUO.
 e importance of the concept of water activity of foods, pharma­ceuticals, and cosmetics cannot be over emphasized. Water activity is a measure of the energy status of the water in a system. More impor­tantly, the usefulness of water activity in relation to microbial growth, chemical reactivity, and stability over water content has been shown.
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4. Getting Started
15
4. Getting Started
Components of your AquaLab
Your AquaLab should have been shipped with the following items:
AquaLab water activity meter
• Calibration Certi cate
• Power cord
RS-232 interface cable
100 disposable sample cups
• Operator’s Manual
Quick Start guide
• Cleaning Kit
3 vials each of the following veri cation solutions:
1.000 a
w
Distilled Water
0.760 a
w
6.0 molal NaCl
0.500 a
w
8.57 molal LiCl
0.250 aw 13.41 molal LiCl
Choosing a Location
To ensure that your AquaLab operates correctly and consistently, place it on a level surface.  is reduces the chance that sample ma­terial will spill and contaminate the sample chamber. Also select a location where the temperature remains fairly stable to avoid tem­perature changes that can a ect accuracy.  is location should be well away from air conditioner and heater vents, open windows, etc. Place the AquaLab in a location where cleanliness can be maintained to prevent contamination of the sample chamber.
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Preparing AquaLab for Operation
After  nding a good location for your AquaLab, plug the power cord into the back of the unit.  e ON/OFF switch is located on the lower left corner of the AquaLab’s back panel. When the AquaLab is turned on, you should see a model name/number screen and then the main screen as shown below.
 e main screen shows the water activity (aw) in the middle of the screen and the sample temperature right below. On the Series 4TEV model you will also see either DEW or CAP indicating whether you are using the dewpoint or capacitance sensor respectively.
NOTE: In order to provide the most accurate readings, your AquaLab should be allowed a 15 minute warm-up period.
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4. Getting Started
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If users have been setup on the instrument, the following screen will appear instead of the main screen. (See Chapter 5 for more informa­tion on administrative settings and user setup).
Select the appropriate user and login to begin.
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5. Menus
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5. Menus
At the top of the display screen there are three tabs: Measurement, Con guration, and Data.  ese tabs indicate the three menus you can access. To change between the tabs press the right most button below the document icon.
 e enter icon is the read or enter button. Once the latch is set to the read position, the document icon will switch to an “X” icon, which allows the user to stop the current reading. During a reading, press­ing enter again will restart the reading.
Measurement Tab
 e measurement tab, as seen above, is the main screen which dis­plays each time you turn on your AquaLab. If this screen doesn’t appear, refer to Chapter 12 for troubleshooting instructions. As mentioned earlier, the water activity and sample temperature are displayed on the screen.
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5. Menus
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Pushing the right or left arrow keys will change the display to a temperature equilibration screen shown below.  is screen shows the temperature di erence between the sample temperature and the lid temperature.
Con guration Tab
When at the con guration screen, pressing the up and down arrow keys moves the cursor through the various con guration options Press the left and right arrows to page through the options.  e enter button will allow you to change the highlighted setting.
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Calibration:
Pressing the Enter button when Calibration is highlighted starts the veri cation process. For more details on the water activity veri ca­tion procedure refer to Chapter 7. Refer to Chapter 10 for moisture content veri cation information (Duo model only). You may also reset the calibration to the factory defaults by highlighting the De­faults option and pressing Enter.  is will reset all options to the way they were when the instrument arrived at your location.
Temperature:
 e default temperature is 25°C. Press the enter button to change the temperature setting.  e AquaLab Series 4TE models may be set between 15 and 50°C by 0.1°C intervals. Using the up and down arrows, set the AquaLab to your desired temperature and press the save button.
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Temp Eq:
 e Temperature Equilibration option allows you to set the level of temperature equilibration desired before the water activity measure­ment begins.  e range is 0.5 to 4.0°C. A setting of 4.0°C begins the measurement immediately (assuming the sample is not >4.0°C above or below the block temperature). A setting of 0.5 °C will cause the instrument to wait until the sample temperature is within <0.5°C of the block temperature before starting the water activity measurement.
Sensor:
In the AquaLab Series 4TEV model only, this option indicates the selected sensor type, either dewpoint or capacitance ( e Series 4 and 4TE models will always be Dewpoint). Pressing Enter when the Sensor option is highlighted allows you to change between a capaci­tance sensor or chilled mirror dewpoint sensor for sampling with or without volatiles, respectively.
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Mode:
Users may choose between single, continuous, or custom mode by pushing the save button.
Single Mode
Single mode reads the sample once, after which the instrument noti­ es you that it is  nished and the water activity and temperature are displayed on the screen.
Continuous Mode
Continuous mode reads your sample until you open the chamber lid or stop the test using the stop button. AquaLab reads the sample, displays the water activity and temperature, then begins another read cycle without further input from the user. Between samples, the ma­chine will signal you with beeps.  is mode eliminates the possibility of moisture exchange with the environment outside the chamber in between readings. A time on the bottom left of the screen tracks the cumulative read time. All readings taken during continuous mode are saved on the instrument’s memory if the autosave feature is se­lected (see Auto Save below). If AquaLab is connected to a computer using AquaLink RG (See Chapter 11), all readings taken during con­tinuous mode will be downloaded to the AquaLink RG software.
Custom Mode
Custom mode allows a sample to be read multiple times until a de­sired level of stability is achieved.  e user determines how many consecutive tests they want to be within a given water activity sta­bility setting. For instance, the customer can choose to have 4 con­secutive tests be within +/- 0.001aw.  e instrument will continue to run tests until it records 4 consecutive tests that are within +/-
0.001aw and then will stop and report the value of the  nal test. If autosave is turned on, all test readings will be saved to the instru-
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ments memory, but only the  nal reading will appear on the main measurement screen. If AquaLab is connected to a computer using AquaLink RG (See Chapter 11), all readings taken during a cus­tom mode test will be downloaded to the AquaLink RG software.
On the mode screen, at the top of the page, will appear the current mode settings with the number of tests appearing  rst, followed by the stability value (∆aw). Pressing enter with the custom mode highlight­ed will allow the number of tests and stability settings to be changed.
To change the number of readings, use the right/left arrow but­tons to highlight the number under Readings, and then use the up and down buttons to change to any value between 2 and 9.
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To change the stability setting, use the right/left arrow buttons to highlight the number under ∆a
w
, and then use the up and down but­tons to change to any value between 0.0005 and 0.0200. To save the settings and  nish, press the save button (to exit without updating, press the cancel button).  e mode screen will now appear with the updated custom settings appearing at the top of the screen. Press the save button to return to the con guration screen and begin using the custom mode (To exit without updating, press the cancel button).
Date:
AquaLab Series 4 models now have an internal calendar and clock.  e time and date are recorded with each water activity reading. Pressing Enter when the Date option is highlighted allows you to set the date in the instrument. Press the left and right arrows to change between the month, day and year. Press the up or down arrows to change any of the individual values.
Time: Pressing Enter when the Time option is highlighted allows you to set the current local time. Press the up or down arrows to change any of the individual values. Press the left or right buttons to change between hour and minutes.  e hour setting automatically changes between AM and PM.
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