Coulter Corporation makes no representation that, upon furnishing this service manual, the holder of the manual will have the necessary
technical capabilities and know-how to properly troubleshoot and repair any of the equipment specified in the manual. Coulter
Corporation assumes no liability whatsoever, including consequential and incidental damages, resulting from improper operation of
Coulter instruments after maintenance of Coulter instruments has been performed by persons not employed by Coulter Corporation.
Furthermore, Coulter Corporation assumes no liability whatsovever for any personal injury or property damage resulting from
maintenance and/or repair of Coulter instruments performed by persons not employed by Coulter Corporation.
HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS
WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
WARNING:Might cause injury.
CAUTION:Might cause damage to the instrument.
IMPORTANT: Might cause misleading results.
"This Service Manual contains confidential information of Coulter Corporation and its receipt or
possession does not convey any rights to reproduce, disclose its contents, or to manufacture, use, or sell
anything it may describe. Reproduction, disclosure, or use without specific written authorization of Coulter
Corporation is strictly forbidden."
Throughout this manual you will encounter the headings WARNING,
CAUTION, IMPORTANT, and Note. These are provided to inform you
of potentially hazardous situations and important or helpful information.
A WARNING indicates a situation or procedure that, if ignored, can
cause serious personal injury. A WARNING will appear in bolded text
for easy identification.
A CAUTION indicates a situation or procedure that, if ignored, can
cause damage to equipment. A CAUTION also appears in bolded text.
IMPORTANT
An IMPORTANT indicates a situation or procedure that, if ignored can
result in erroneous test results. An IMPORTANT also appears in bolded
text.
Note
A Note contains information that is important to remember or helpful in
performing a procedure.
1.2ELECTRONIC SAFETY PRECAUTIONS
WARNING
Remove rings and other metal jewelry before performing maintenance
or service on the electronic components of instrument.
PN 4237161A (July 1992)1-1
WARNINGS AND CAUTIONS
1.3BIOLOGICAL SAFETY
CAUTION
To prevent damage to delicate electronic components, always be sure
power is OFF before removing or replacing printed circuit boards and
components.
WARNING
To prevent possible injury or biological contamination, service
personnel must wear gloves and eye protection when servicing the
instrument with the doors open.
Use care when working with pathogenic materials. Means must be
available to decontaminate the instrument, provide ventilation, and to
dispose of waste liquid. Refer to the following publications for further
guidance on decontamination.
Biohazards Safety Guide, 1974, National Institute of Health.
Classifications of Etiological Agents on the Basis of Hazards, 3d ed.,
June 1974, Center for Disease Control, U.S. Public Health Service.
In areas of high risk, operator must be trained in person. Telephone
instruction is prohibited.
1-2PN 4237161A (July 1992)
2.1SPECIFICATIONS
Dimensions/Weight
Power
INSTRUMENT DESCRIPTION 2
Height49.5 cm (19.5 in.)
Width43.2 cm (17 in.)
Depth40.6 cm (16 in.)
Weight34 Kg (75 lb)
Input
•100 Vac (±10%) 50/60 Hz
•120 Vac (±10%) 50/60 Hz
•220 Vac (±10%) 50/60 Hz
•240 Vac (±10%) 50/60 Hz
Consumption
Less than 200 watts
Ambient Operating Temperature
16°C to 32°C (60°F to 90°F)
Humidity
0 to 95% without condensation
Recommended Reagents
COULTER MICRO-PAK (diluent and lytic reagent) P/N 8547007
COULTER CLENZ
Controls and Calibrators
4C®PLUS Cell Control Tri Pac P/N 7547003
®
S-CAL
Calibrator Kit P/N 7547005
®
Cleaning Agent P/N 8546931
PN 4237161B (April 1993)2-1
INSTRUMENT DESCRIPTION
Fluid Volumes
•Whole Blood Aspirated: 12 µL
•ISOTON
•LYSE S
®
III diluent consumption per cycle: 18.64 mL
®
III Diff reagent consumption per cycle: 415 µL
Interfering Substances
Refer to Product Reference Manual for known interfering substances
2.2INTRODUCTION TO THE COULTER MicroDiff
Function
The MicroDiff is an automated hematology analyzer for in vitro
diagnostic use in clinical laboratories. The MicroDiff reports a 16
parameter Complete Blood Count, including a six parameter WBC
differential, from whole blood.
Mode of Operation
The MicroDiff has one operating mode. The sample is introduced to the
instrument by removing the cap from the sample tube, presenting the
sample to the aspirator probe, and starting the cycle.
2.3DIFFERENTIAL MEASUREMENT
The MicroDiff performs WBC differentials from whole-blood samples. It
provides lymphocyte, monocyte and granulocyte percentages and
absolute numbers using Coulter histogram differential technology.
The white blood cells are counted and sized for the WBC histogram in a
single 100-µm WBC aperture.
2.4OPERATIONAL OVERVIEW
The cycle is started by pressing the 〈ASP〉button on the keypad:
1.The aspirate syringe is driven down to aspirates 12 µL of sample and
a small amount of air.
2.The diluent syringe fills with diluent for the WBC dilution.
3.The bath drains.
2-2PN 4237161B (April 1993)
OPERATIONAL OVERVIEW
4.The aspirator probe moves to the bath and dispenses the sample into
the bath. The diluent syringe dispenses diluent through the probe
into the bath making a 215:1 dilution.
5.Mixing bubbles from the air pump (PM1) enter the bath to mix the
solution.
6.The aspirate syringe is driven down again to aspirate 100 µl of the
dilution from the bath into the probe, where it is held for the
RBC/Plt dilution.
7.The lyse pump (PM5) dispenses 415 µL of lyse to the bath for a final|
WBC dilution of 251:1 and the sample is mixed again.
8.The WBC solution is drawn through the WBC aperture for 12 s to
count and size the WBCs.
9.After WBC analysis, and just before the WBC sample drains from
the bath, the Hgb reading is taken.
2.5DILUTER
General
10. The bath is drained and rinsed and Hgb Blank is taken.|
11. The diluent syringe fills with diluent for the RBC/Plt dilution.
12. The 100 µL of the 215:1 solution, stored in the aspirate probe, is
dispensed into the bath with diluent to obtain a final RBC/Plt
dilution of 6250:1.
13. Mixing bubbles from PM1 enter the bath to mix the solution.
14. The RBC/Plt dilution is drawn through the RBC/Plt aperture for 12 s
to count and size the RBCs and Plts.
15. The bath is drained and rinsed.
16. The aperture is zapped, and the system is prepared for the next test|
sequence.
The diluter section of the MicroDiff processes the sample for analysis.
The locations of the components are illustrated in Figure 2.1.
PN 4237161B (April 1993)2-3
INSTRUMENT DESCRIPTION
2
1
18
20
3
10
ON/OFF
SWITCH
19
9
222117
1. LCD DISPLAY
2. KEYPAD
3. DISK DRIVE
4. ASPIRATOR PROBE
5. ASPIRATION SYRINGE
6. DILUENT SYRINGE
7. VL2 DILUENT VALVE
8. LYSE PUMP PM5
9. LYSE DECTECTOR
10. VACUUM REGULATOR
11. VACUUM ISOLATOR
12. VL1 COUNT VALVE
13. BATH-WBC APERTURE
14. BATH-RBC APERTURE
15. HGB LED
16. LV3 DRAIN SOLENOID
17. AIR PUMP PM1
18. LV4 AIR MIX SOLENOID
19. DILUENT RESERVOIR
20. DILUENT PUMP PM2
21. RINSE PUMP PM3
22. WASTE PUMP PM4
7
5
6
11
124161315148
7161-01
Figure 2.1 Diluter Components|
The MicroDiff uses two syringes driven by stepper motors to aspirate
sample and deliver diluent. The syringe volumes are:
•Aspirate syringe100 µL
•Diluent syringe5000 µL
The instrument uses four stepper motor driven peristaltic pumps to
generate mixing bubbles, drain the bath, deliver rinse to the bath, and
draw diluent from diluent pack into the diluent reservoir.
Two stepper motor driven switching valves, Count Valve 1 and Dilution
Valve 2, control the flow and direction of the fluidics and pneumatics.
2-4PN 4237161B (April 1993)
Count Valve 1 (VL1)
The count valve is a three-position valve that routes count vacuum to the
WBC and RBC apertures.
Dilution Valve 2 (VL2)
The dilution valve is a four-position valve that:
•Routes diluent to the bath through the aspirate probe.
•Provides a path to the diluent supply to fill diluent syringe.
•Routes the sweep flow diluent from the diluent reservoir through
the sweep flow can to the RBC aperture.
Stepper Motor Driver Cards
Two Stepper Motor Driver cards, in the Diluter, provide the drive voltage
to the stepper motors, the opto switches, the lyse pump, solenoids, and
the cycle counter.
DILUTER
Stepper Motor Driver Card 1 drives the following components:
• Diluent Pump• Air Pump
• Rinse Pump• Waste Pump
• Lyse Pump• Air Mix Solenoid
• Diluent Reservoir Sensor• Lyse Level Sensor
• Cycle Counter
Stepper Motor Driver Card 2 drives the following components:
The location of the electrical connections and test points on the Stepper
Motor Driver Cards is shown in Figure 2.2. Tables 2.1 and 2.2 list the
connectors and test points.
J8Not UsedTraverse Motor
J10MSMC 1 J2MSMC 2 J2
J12Air Mix MotorDiluent Valve Motor
J13Not UsedDiluent Valve Opto
J14Diluent Level SenseNot Used
DILUTER
J15Diluent Fill MotorNot Used
J17MSMC 1 J3MSMC 2 J3
J18Resistor BankResistor Bank
J19Rinse MotorAspirate Syringe Motor
J20Not UsedAspirate Syringe Opto
J21Lyse Level SenseDiluent Syringe Opto
J22Waste MotorDiluent Syringe Motor
J23Resistor BankResistor Bank
J25Not UsedNot Used
J26Not UsedIso Chamber VentValve
J27Air Mix SolenoidVacuum Sense Vent Valve
J28Cycle CounterVacuum Pump ON Signal
J29Lyse PumpDrain Solenoid
J30Not UsedPower ON to Relay Card
J31Not Used+12 Volts
PN 4237161B (April 1993)2-7
INSTRUMENT DESCRIPTION
Table 2.2 Stepper Motor Driver Card Test Points
Test PointsDriver 1Driver 2
TP3GroundGround
TP4Not UsedAspirate Syringe Opto
TP5Lyse Level SenseDiluent Syringe Opto
TP6 - TP13Stepper Motor PhasesStepper Motor Phases
TP14Not UsedDiluent Valve Opto
TP15Diluent Level SenseNot Used
TP16 - TP23Stepper Motor PhasesStepper Motor Phases
TP24Not UsedCount Valve Opto
TP25Not UsedTraverse Opto
TP26 - TP33Stepper Motor PhasesStepper Motor Phases
TP34+24 Volts+24 Volts
2.6VENT VALVE VACUUM SENSE CARD
The Vent Valve Vacuum Sense Card (VVVS) is responsible for
monitoring the 6-in. Hg of vacuum used for count, venting the isolator
chamber when in drains, and venting the vacuum transducer to obtain
an atmosphere offset.
The output of the VVVS goes to the IORA card. the voltage for 6 in. Hg
of vacuum is 4.286 volts plus the measured atmosphere offset voltage.
2.7ELECTRONICS ASSEMBLY
General
The Electronics Assembly of the MicroDiff is contained in a hinged
compartment at the top of the instrument. It consists of the following
subassemblies:
• Motherboard with 80386 microprocessor
• 200 watt switching power supply
• Front panel with keypad and LCD display
• 1.44 MB 3½in. floppy disk drive
• Data Acquisition Board (DAC)
• Multi Stepper Motor Controller Card (MSMC)
• Analog/Peripheral Board (IORA)
• Vacuum Sensor Amp Board (PVAC)
2-8PN 4237161B (April 1993)
Motherboard
SERIAL 1
SERIAL 2
3
5
2
4
1PARALLEL
FLOPPY DISK
SWITCH
BATTERY
IDE HARD DISK
80387SX
4
1
4MX9 SIM M
SUPPORT
JUMPER
8742
27512
RESET
SPEAKER
KEYLOCK
IDE ACTIVITY
SXCAT DIAGRAM
7161016A
TURBO
LED
TURBO
SWIT CH
ELECTRONICS ASSEMBLY
The Motherboard (Figure 2.3) is an AT compatible 80386 computer. The
disk controller and serial I/O port are resident on the board. Program
software is not resident in EPROMs on the motherboard. The program is
loaded from the diskette drive into the computer’s memory.
Figure 2.3 Motherboard
Switch|Description|
SW1-1|Close (On) to enable Onboard Battery.|
Open (Off) to enable Offboard Battery.|
SW1-2|Close (On) to Enable Battery.|
Open (Off) to Disable Battery.|
Must be closed for normal system operation.|
SW1-3|Close (On) for additional wait states on IDE interface.|
Open (Off) for no additional wait states.|
SW1-4|Close (On) for Color Adapter.|
Open (Off) for Monochrome Adapter.|
This switch setting does not matter when using VGA or|
PN 4237161B (April 1993)2-9
EGA adapters.|
Linear Power Supply
ELECTRONICS ASSEMBLYINSTRUMENT DESCRIPTION
The Linear Power Supply (Figure 2.4) is a self contained unit located
below the Computer Chassis at the bottom of the Main Chassis
Assembly. The unit is accessible with the hinged electronics
compartment open. The Power Supply provides all the necessary dc and
ac voltages to operate the unit. The supplied voltages are:
•±15 Vdc for the analog circuitry
•+24 Vdc for the stepper motors and pneumatic solenoids
•+240 Vdc to generate aperture current and zap volts
•0.02 amp constant current source for the Hgb LED.
The unit supplies dc power to the following assemblies:
•DATA ACQ and IORA Boards located in the electronics
compartment.
•PVAC Board, and the Hgb LED assembly located in the diluter
panel.
•Motor Driver boards located in the Main Chassis.
The unit also supplies the ac power for the Vacuum Pump, Switching
Power Supply, and the 24-volt Power Supply.
Power Supply Components
Power Supply Board: This board consists of rectifiers; filters and
regulators for ±15 V and +240 V; a constant current source for the Hgb
LED; connectors for all inputs, outputs and test points.
Power Transformer T1: T1 provides stepped down ac power to the power
supply board for the +15 volt supply, the -15 volt supply, the 240 volt
supply and the constant current source for the Hgb LED supply which is
driven from the +15 volt supply.
24V Power Transformer T2: T2 provides stepped down ac voltage for the
+24v Power Supply.
2-10PN 4237161B (April 1993)
ELECTRONICS ASSEMBLY
FUSE HOLDERREGULATOR CARD
LINE FILT ER
OUTLET
RELAY CA RD
24V TRANSFORMER
MAIN TRANSFORMER
BRIDGE RECTIFIER
24V FILTER CAPACITOR
7161-04
J
2
TP9
APP C URRE NT
Figure 2.4 Linear Power Supply
J3
J4
J1
Figure 2.5 Regulator Card
Hgb
Hgb Ref.
+15
Gnd
-15
Gnd
240V Gnd
240V
TP7
TP6
TP5
TP4
TP3
TP8
TP2
TP1
7161-05
PN 4237161B (April 1993)2-11
ELECTRONICS ASSEMBLYINSTRUMENT DESCRIPTION
+24 Rectifier and Filter: Provides +24 Vdc for motors and solenoids.
Vacuum Pump Solid State Relay: Connects the input ac voltage to the
vacuum pump.
+24-V Relay: Connects the input ac voltage to the +24-Volt power
transformer. The signal to operate the relay comes from the MSMC Card
through the motor driver.
AC Line Filter: Filters the input ac voltage before going to the
transformers and switching power supply.
Fuses: Control the ac line voltage to the input of the instrument.
Terminal Block: Provides ac line voltage selection (see Table 2.3).
Table 2.3 Terminal Block Connections
Input VoltageRelay Card AC WirePositionJumper
100White
Black
120White
Black
220White
Black
240White
Black
1
4
1
6
1
4|
1
6|
1&2
3&4
1&2
5&6
2&5
2&5
The Power Transformer T1 has three secondaries that provide ac voltage
to all the supplies. The high voltage winding is surrounded by an
electrostatic shield to reduce noise induced by the ac line. All the
secondary windings of the transformer are internally fused.
The ac voltage is then distributed to the power supply card where the ac
is full wave rectified, filtered, and regulated. The regulated supplies are
protected against overload and short circuit by current foldback and
thermal shutdown.
Two input signals to the Power Supply Card come from the DAC Card.
These signals are the aperture current ON command and aperture
current control. The aperture current ON command turns on the high
voltage regulator when aperture current is required.
The aperture current control is a signal between 0 and 5 Volts that
controls the output of the high voltage regulator. The output of this
regulator controls the gain of the pre-amp section on the DAC Card.
The Power Supply has two fuses, F1 and F2, rated at 2.0 A for 100 Vac to
120 Vac or 1.0 Amps for 200 Vac to 240 Vac.|
2-12PN 4237161B (April 1993)
Switching Power Supply
The Switching Power Supply is in the top chassis and provides +5 Vdc
and +12 Vdc to the CPU and associated electronics. The switching
supply also has the POWER ON switch for the system and a cooling fan.
The ac input voltage is switch selectable for 90 Vac to 130 Vac or 180
Vac to 260 Vac. The voltages developed are plus and minus 5 volts and
plus and minus 12 volts.
There are 5 connectors coming from the Switching Power Supply. A label
on the Switching Power Supply list the voltage and current for each
connector and connector pin.
The power connectors P4 and P5 must not be switched!! If they are
reversed the computer motherboard may be damaged. P4 should be
towards the rear of the board and P5 towards the front. Another way to
check proper connection is thatthecommonblackwiresfor each of the
connectors will be next to each other in the middle .
ELECTRONICS ASSEMBLY
CAUTION
Data Acquisition Card
The destination of the Switching Power Supply connectors are: