Corning 450 Instruction Manual
450 pH/ion Meter
Instruction Manual
This manual contains complete instructions for setting up and using
the 450 pH/ion meter. Applications information is also available.
The information contained in this manual was correct at the time of
going to print. However, we continue to improve products and reserve
the right to change specifications, equipment and maintenance
procedures at any time.
This manual is copyrighted, and all rights are reserved. No part of
this manual may be reproduced by any means or in any form without
prior consent in writing.
The power supply unit is classed as IEC Class II equipment
(equipment providing an adequate degree of protection against
electric shocks, in which additional safety precautions, for example,
double or reinforced insulation, are included). The 450 is intended for
use by persons knowledgeable in safe laboratory practices. If the 450
is not used in accordance with these instructions for use, the
protection provided by the equipment may be impaired.
The 450 is suitable for direct current only.
This equipment generates, uses and can radiate radio frequency
energy and if not installed and used in accordance with the
instruction manual, may cause interference with radio
communications. It has been tested and found to comply with the
limits for a Class A computing device pursuant to Subpart J of
Part 15 of FCC Rules, which are designed to provide reasonable
protection against such interference when operated in a commercial
environment. Operation of this equipment in a residential area is
likely to cause interference in which case the user at his own expense
will be required to take whatever measures may be required to
correct the interference.
There are no user replaceable parts in the 450 or power supply unit.
Do not remove the covers.
109120-1 Rev. A, 11/96
1
Contents
1 Set Up
1.1 Unpacking and Installation ................................................ 5
1.2 Input and Output Connections........................................... 6
1.3 Display and Controls ......................................................... 7
1.4 Programming Menu Overview........................................... 8
2 Operation
2.1 Calibrating a pH Electrode .............................................. 10
2.1.1 pH Measurements ........................................................... 11
2.2 mV Measurements .......................................................... 11
2.2.1 Absolute mV .................................................................... 11
2.2.2 Relative mV ..................................................................... 12
2.3 Calibrating an Ion Selective Electrode ............................ 12
2.3.1 Concentration Measurements ......................................... 13
2.4 Automatic Endpoint (Auto Read)..................................... 14
2.5 Temperature Measurements ........................................... 15
2.6 Karl Fischer Titrations ..................................................... 16
2.7 Chart Recorder ................................................................ 16
2.8 Interfacing via the RS232 Output .................................... 17
3 Meter Programming
3.1 Calibration Menu ............................................................. 20
3.1.1 Calibration Point Setting.................................................. 20
3.1.2 Last Calibration Recall .................................................... 21
3.1.3 Isopotential Point Setting (pH) ........................................ 21
3.2 Display Menu................................................................... 22
3.2.1 Channels to Display......................................................... 22
3.2.2 Units for Concentration to Display................................... 22
3.2.3 Resolution for pH to Display............................................ 23
2
109120-1 Rev. A, 11/96
Contents (cont)
3 Meter Programming (cont)
3.3 System Menu................................................................... 24
3.3.1 Time and Date ................................................................. 24
3.3.2 Language......................................................................... 24
3.3.3 Baud Rate........................................................................ 25
3.3.4 Beeper on/off ................................................................... 25
3.4 Temperature Menu.......................................................... 26
3.4.1 Temperature Probe Selection ......................................... 26
3.4.2 Manual Temperature Set................................................. 26
3.5 Limits Menu ..................................................................... 27
3.5.1 Alarm Visual and Audible On/Off .................................... 27
3.5.2 Set High/Low Limits for pH.............................................. 27
3.5.3 Set High/Low Limits for Temperature.............................. 28
3.6 Datalog Menu .................................................................. 29
3.6.1 Datalogger On/Off ........................................................... 29
3.6.2 Timer Setting ................................................................... 29
3.6.3 Recall Logged Data......................................................... 30
3.6.4 Clearing Logged Data ..................................................... 31
3.7 GLP (Good Laboratory Practice) Menu........................... 32
3.7.1 GLP On/Off...................................................................... 32
3.7.2 Calibration Alarm Setting................................................. 32
3.7.3 Entering an ID Number.................................................... 33
3.8 Diagnostics Menu............................................................ 34
3.8.1 Meter Self Test ................................................................ 34
3.8.2 Meter Reset ..................................................................... 35
109120-1 Rev. A, 11/96
3
Contents (cont)
4 Support Information
4.1 Basic Theory.................................................................... 36
4.2 Operating Hints................................................................ 37
4.3 Maintenance .................................................................... 38
4.3.1 Meter Maintenance.......................................................... 38
4.3.2 Electrode Maintenance.................................................... 38
4.4 Problem Solving .............................................................. 39
4.4.1 Meter Test ....................................................................... 39
4.4.2 Meter Error Codes ........................................................... 40
4.4.3 Electrode Problem Solving .............................................. 41
4.5 Meter Specifications ........................................................ 43
Consumables and Accessories ............................... inside rear cover
Warranty .................................................................. inside rear cover
4
109120-1 Rev. A, 11/96
1.1 Unpacking and Installation
1. The main carton will contain the following:
Meter with shorting (test) clips in the input sockets
Meter Dust Cover
Power Supply
This Instruction Manual
Memo Cards, English, French, German, Italian,
Spanish
Guide to ISE Measurements
Warranty Card
Calibration Certificate
2. If you ordered the meter with an electrode, electrode arm
and accessories, the top tray of the main carton will
contain these items. Refer to the accessories packing sheet
for a list of the additional components.
3. Attach the electrode arm to the pH/ion meter:
a. The electrode arm can be attached to the left or right-
hand side. Using the screwdriver supplied remove the
appropriate end cap from the pH/ion meter.
b. Slide the arm base into the recess and tighten the fixing
screw. Replace the end cap.
1
c. Fit the electrode arm onto the post. Adjust the tension
screw as required.
electrode arm
arm base
fixing screw
4. Fill in and return the warranty card. For your record make
a note of the serial number, date of purchase and supplier
on the inside front cover of this manual.
109120-1 Rev. A, 11/96
tension screw
end cap
screwdriver
5
1.2 Input and Output Connections
1
DC - power
supply unit
socket
DATA - data
output socket,
see Section 2.8
for interfacing
details
1. Disconnect the shorting clips from the pH sockets and
2. Connect the reference electrode(s), if using, to the REF
KF - polarizing
current source
for Karl Fischer
titrations
REC - recorder
output socket,
see Section 2.7
for details
retain by clipping over the socket. If you are using two
sensing electrodes connect them to Channel A and B. If you
are using one electrode, connect it to Channel A.
socket(s).
ATC - ATC (automatic temperature
compensation) probe socket
pH - combination or pH electrode
sockets, Channel A & B
AB
REF -
reference
electrode
sockets,
Channel A & B
PT - PT1000
(automatic
temperature
compensation)
probe sockets
3. If you are using an ATC probe connect it to the ATC socket.
If you are using a PT1000 probe connect it to the PT
sockets.
4. Connect the power supply unit to the DC socket.
5. When the 450 is powered up it carries out a display self
test.
6
109120-1 Rev. A, 11/96
1.3 Display and Controls
1
mode
prog
auto
read
on/off
cal
read
enter
arrows
numeric
keypad
Selects pH, mV, relative mV or concentration mode (for
Channel A or Channel B).
Accesses software menu from current mode. Exits
software␣ menu one step at a time.
Selects automatic endpoint detection.
Turns the display off, and places the meter in standby
mode. Wakes the meter up.
Starts a calibration sequence in pH, relative mV and
concentration modes.
Starts sample measurement in all modes. Press again to
freeze display in manual endpoint mode. Starts and stops
timed data logging. Returns to measurement from
software␣ menu.
Selects options in software menu, and enters data or
selection.
Used to move around software menu.
Data entry keys, to set calibration buffers, date/time,
high/low limits etc. Can also be used to move around
software menu quickly.
109120-1 Rev. A, 11/96
7
1
1.4 Programming Menu Overview
2
prog
2
beeper clear data
Use the arrow keys to move around the software menu.
The numeric keypad can also be used as quick access keys. For example, to
3
change the display resolution to X.XX, press then (to access menu 2),
(to access sub routine 3), (to select option 2).
This is useful when operating the 450 remotely from a PC, see Section 2.8.
<
<
<
12 3 4 5 678
calibration display system temp limits datalog GLP diagnostics
cal points channel time/date ATC type alarm on/off on/off self test
last cal conc units language manual set limits timer cal alarm reset
The 450 uses a menu system of software for the set up functions. There are eight main menus, these
are: calibration, display, system, temp, limits, datalog, GLP, diagnostics. This chart shows the eight
8
prog
main menus and their sub routines.
1
iso pH resolution baud temp recall data ID no.
2
3
4
109120-1 Rev. A, 11/96
enter
<
1.4 Programming Menu Overview (cont)
To access the menus:
press the display shows three menus at a time (if you are
prog
using both measurement channels (A and B), you have
to select which channel you want to set up).
< calibration display system >
<
Press and to move round the menus.You can move
< display system temp >
To access the menu sub routines, press or when
your required menu is highlighted,
e.g. to access the display menu sub routine press
< display system temp >
<
channel
conc units
pH resolution
either way, and the menu wraps round at
each end. Each time you press an arrow key
the next menu appears, e.g. press
enter
Use to move down through the
<
sub routines.
<
enter
<
<
To access your chosen sub routine press or
enter
1
To choose from options displayed use and then
enter
press
Before you can enter or change a value the value must be
flashing, for example: cal 1 = 7.000
Press Change the flashing value using the keypad, then
Press to return directly to measurement.
enter
press again.
read
109120-1 Rev. A, 11/96
enter
<
<
9
2
2.1 Calibrating a pH Electrode
If you are using an ATC probe (or an electrode incorporating
ATC), temperature is being measured and the buffer value is
being corrected for temperature (see Section 2.5). If you are not
using an ATC in the system, the temperature can be entered
manually (see Section 3.4.2).
If you have the 'auto read' feature (see Section 2.4) turned on,
the meter will automatically endpoint when the reading is stable.
To manually endpoint and freeze the display press
1 point calibration
Place the tip of the electrode in the first buffer and press
If you are displaying both channel A and B, select the one you
are calibrating and press
The calibration buffer screen will display. Five calibration
buffers are shown on the display. If you want to use other
buffers, you will need to enter the values (see Section 3.1.1). At
the measurement endpoint a tick (✔) will appear next to the
calibration buffer and the electrode offset will display (in mV).
enter
read
cal
Rinse the tip of the electrode with distilled or deionized water
and goto pH Measurements (Section 2.1.1) or 2 point calibration.
2 point calibration
Place the tip of the electrode in the second buffer and press
At the measurement endpoint a tick (✔) will show next to the
buffer and the electrode slope will display.
Rinse the tip of the electrode with distilled or deionized water
and goto pH Measurements (Section 2.1.1) or 3 point calibration.
10
109120-1 Rev. A, 11/96
cal
2.1 Calibrating a pH Electrode (cont)
3, 4 and 5 point calibration
Repeat above procedure using the third, fourth and fifth buffers.
NOTES The five buffers can be selected for calibration in any
order.
<
Use and to select the buffer you want to
<
use for the calibration and press
2.1.1 pH Measurements
NOTE Good laboratory practice dictates that electrodes
should be calibrated at least daily.
Place the tip of the electrode in the sample and press to
start the measurement. The decimal point will be
flashing whenever the electrode is reading.
If you are using the 'auto read' function the display freezes when
a stable endpoint is reached (see Section 2.4). To manually freeze
the display when the reading is stable, press
2.2 mV Measurements
The millivolt capability of pH meters can be used to determine the
actual slope of pH electrodes but is most frequently used for doing
potentiometric readings or titrations with Platinum OxidationReduction electrodes.
2.2.1 Absolute mV
cal
read
read
2
mode
Press until mV shows at the top of the display.
Put the tip of the electrode in the sample and press The
meter will display absolute millivolts.
When the reading is stable, press to freeze the display (or
use 'auto read', see Section 2.4).
109120-1 Rev. A, 11/96
read
read
11
2.2 mV Measurements (cont)
2.2.2 Relative mV
mode
Press until Rel mV shows at the top of the display.
Put the tip of the electrode in the reference solution and press
The meter will be displaying absolute millivolts.
cal
2
When the reading is stable, press to freeze the display (or
use 'auto read', see Section 2.4).
The display will update to a relative mV value 0.0 mV.
Put electrode in test sample and press (or use
'auto read'). The meter will show the change in mV from 0.0 mV.
2.3 Calibrating an Ion Selective Electrode
If you have the 'auto read' feature (see Section 2.4) turned on,
the meter will automatically endpoint when the reading is stable.
To manually endpoint and freeze the display, press
To calibrate an electrode:
Press If you are using both measurement channels (A
Five calibration standards are shown on the display. If you want
to use other standards, you will need to enter the values (see
Section 3.1.1).
cal
and B), select the one you want to calibrate
using and then␣ press
<
read
<
read
read
enter
1 point calibration
Place the tip of the electrode in the first standard. At the
measurement endpoint a tick (✔) appears.
The electrode offset is displayed.
To return to sample measurement press
12
read
109120-1 Rev. A, 11/96
2.3 Calibrating an Ion Selective Electrode (cont)
2 point calibration
To continue with a 2 point calibration:
Place the tip of the electrode in the second standard and
press
When the display freezes the electrode slope value will be
displayed.
To return to sample measurement press
3, 4 and 5 point calibration
Repeat the procedure using the third, fourth and fifth calibration
standard.
NOTES The standard set for the first calibration standard is
2.3.1 Concentration Measurements
We recommend regular calibration before sample measurements
(see Section 2.3). To measure concentration: Place the tip of the
electrode(s) in the sample and press
cal
read
the isopotential point.
The five standards can be selected for calibration in
any order.
<
Use and to select the standard you want to
use for the calibration and press
<
cal
read
2
The display will show the concentration of the sample. The units
can be changed (see Section 3.2.2). If you are using a
temperature probe, temperature of the sample will be displayed.
If you are using 'Auto' endpoint the display will freeze
automatically (see Section 2.4). To endpoint manually
press
read
109120-1 Rev. A, 11/96
13
2.4 Automatic Endpoint (Auto Read)
The 'auto read' function checks the electrode output for stability
and automatically freezes the display when the reading appears
stable. It can be used during calibration and routine
measurements.
2
A
read
auto
read
auto
read
Turn auto endpoint on by pressing
The auto endpoint indicator will appear at the top of the
meter display. While the meter is reading, the decimal will flash.
When the endpoint is reached, it will stop flashing.
To start another reading press
To cancel auto endpoint press again.
14
109120-1 Rev. A, 11/96
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