Cole-Parmer 83057-01, 83057-06, 83059-10, 83059-15 Instruction Manual
SPECTROPHOTOMETER
Cole Parmer
USER’S MANUAL
MODELS
83057-01; 83057-06
83059-10; 83059-15
V 2.0
Cole Parmer Instruments Company.
625 East Hills, IL 60061
800-323-4340
TABLE OF CONTENTS
General information……………………………………………………………… 3
Safety………………………….........................................................……… 3
Electrical………………………………………………………………........ 3
Warning…………………………………………………………………….. 3
Performance………………………………………………………………… 4
Radio Interference………………………………………………………….. 4
Introduction…........................................................................……………… 4
Working Principle.......................................................................…………… 4
Unpacking Instructions.....................................................………………….. 5
Specifications….............................................................................................. 5
Installation.............................................................................…….................. 5
Operational Panel....................................................................……................ 7
Description of Keys....................................................................……............ 7
Operation Instruction............................................................……………................... 8
Spectrophotometer Initialization ......................................................................... 8
BASIC MODE-%T/Abs Measure...............................................……………. 11
Quantitative Test.......................................................................……………… 15
Define/Establish Test Method……………………………………….. 15
By Standards………………………………………………… 15
By Known Factors…………………………………………… 23
Run Tests ……………………………………………………………. 28
System Setup…………………………………………………………………….. 31
Edit clock …………………………………………………………… 31
Dark Current………………………………………………………….. 33
WL Calibration ……………………………………………………….. 34
Language ……………………………………………………………… 36
WL Correction………………………………………………………… 37
Firmware Version …………………………………………………….. 37
PC LINK………………………………………………………………………. 37
Wavelength Calibration…………….……………………………….……………….. 37
Holmium Oxide Filter Method………………………………………………… 37
Didymium Filter Method……………………………………………………… 38
Absorbance Accuracy Checks………………………………………………… 39
Stary Light Check……………………………………………………………… 39
Trouble Shooting………………………………………………………….................. 41
Error Codes Definitions……………………………………………………………… 42
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1. General information
The apparatus described in this manual is designed to be used by properly trained personnel in a
suitable equipped laboratory. For the correct and safe use of this apparatus it is essential that
laboratory personnel follow generally accepted safe procedures in addition to the safety
precautions called for in this manual.
The covers on this instrument may be removed for servicing. However, the inside of the power
supply unit is a hazardous area and its cover should not be removed under any circumstances.
There are no serviceable components inside this power supply unit. Avoid touching the high
voltage power supply at all times.
Some of the chemicals used in spectrophotometry are corrosive and/or inflammable and samples
may be radioactive, toxic, or potentially infective. Care should be taken to follow the normal
laboratory procedures for handling chemicals and samples.
Safety
Read the following before installing and using the instrument and its accessories.
Electrical
Before switching on the apparatus, make sure it is set to the voltage of the local power supply.
The power cord shall be inserted in a socket provided with a protective earth contact. The
protective action must not be negated by the use of an extension cord without a protective
conductor.
Warning
Any interruption of the protective conductor inside or outside the apparatus or disconnection of
the protective earth terminal is likely to make the apparatus dangerous. Intentional interruption
is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall be made
inoperative and be secured against any unintended operation. NEVER touch or handle the
power supply due to the high voltage.
The protection is likely to be impaired if, for example, the apparatus
• Shows visible damage
• Fails to perform the intended measurements
• Has been subjected to prolonged storage under unfavorable conditions.
• Has been subjected to severe transport stresses
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Performance
To ensure that the instrument is working within its specification, especially when making
measurements of an important nature, carry out performance checks with particular reference to
wavelength and absorbance accuracy. Performance checks are detailed in this manual.
Radio Interference
For compliance with the EMC standards referred to in the EC Declaration of Conformity, it is
necessary that only shielded cables are used when connecting the instrument to computers and
accessories.
Introduction
Cole-Parmer Spectrophotometer is a single beam, general purpose instrument designed to meet
the needs of the Conventional Laboratory, They are ideal for various applications, such as:
Biochemistry, Petro-chemistry, Environmental Protection, Food and Beverage Labs, Water and
Waste Water Labs and other fields of quality control and research.
The spectrophotometer features a digital display, easy operation and wavelength range of 325nm
to 1000nm for visible and 200nm to 1000nm for UV model. It is ideal for measurements in the
visible wavelength region of the electromagnetic spectrum and UV model in ultraviolet and
visible wavelength region.
Working Principle
The spectrophotometer consists of five parts: 1) Halogen and deuterium (UV model only) lamp
to supply the light; 2) A Monochromator to isolate the wavelength of interest and eliminate the
unwanted second order radiation; 3) A sample compartment to accommodate the sample
solution; 4) A detector to receive the transmitted light and convert it to an electrical signal; and
5) A digital display to indicate absorbance or transmittance. The block diagram below illustrates
the relationship between these parts.
Block diagram for the Spectrophotometer
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In your spectrophotometer, light from the lamp is focused on the entrance slit of the
monochromator where the collimating mirror directs the beam onto the grating. The grating
disperses the light beam to produce the spectrum, a portion of which is focused on the exit slit of
the monochromator by a collimating mirror. From here the beam is passed to a sample
compartment through one of the filters, which helps to eliminate unwanted second order
radiation from the diffraction grating. Upon leaving the sample compartment, the beam is
passed to the silicon photodiode detector and causes the detector to produce an electrical signal
that is displayed on the digital display.
The spectrophotometer incorporates USB bi-directional port for connecting to a PC for using the
Application Software (not included with the instrument).
The RS232 Port is for use with RS232 printer and for firmware (built-in software) upgrade.
Unpacking Instructions
Carefully unpack the contents and check the materials against the following packing list to
ensure that you have received everything in good condition:
Packing List
Unless otherwise specially ordered the spectrophotometer package should include the following
items.
Description: ________________________ Quantity
Spectrophotometer...........……................. …............................................................ 1
Power Cord…………………………………......………………………………….. 1
Cuvettes, Glass ..................................……............... …............................................ Set of 4
Cuvettes, Quartz(UV model only)………………………………………………… Set of 2
Dust Cover...................................…….......…. …..................................................... 1
Manual................................................………............................................................. 1
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Specifications for 83057-01/83057-06 and 83059-10/83059-15
Wavelength Range 325-1000nm 200-1000nm
Spectral Bandpass 4nm 4nm
Wavelength Accuracy + 2 nm +2 nm
Wavelength Repeatability + 1nm +1nm
Stray Radiant Energy <0.3 @ 340 and 400nm <0.3 @ 220 and 340nm
Photometric Range 0 to 125%T
Photometric Accuracy + 0.004@0.5A + 0.004@0.5A
Display Graphic LCD 128x64 LCD Graphic 128x64
Control and Data Entry Touch Button Keypad Touch Button Keypad
83057-01/83057-06 83059-10/83059-15
0 to 125%T
-0.3 to 2.5 Abs
-9999 to 9999
0.3 to 2.5 Abs
-9999 to 9999
USB Port For PC connection (requires PC
software)
Data output For RS232 printer and firmware
upgrade
Power Requirements 90-240Vac, 50-60 Hz 90-240Vac, 50-60 Hz
Dimensions 550W x 400D x 270H (mm) 550W x 400D x 270H (mm)
Light Source Tungsten Halogen Tungsten Halogen/Deuterium
Weight 30 lbs. /14kg 46 lbs. /21kg
For PC connection (requires PC
software)
For RS232 printer and firmware
upgrade
Installation:
1. After carefully unpacking the contents, check the materials with the packing list to ensure that
you have received everything in good condition.
2. Place the instrument in a suitable location away from direct sunlight. In order to have the
best performance from your instrument, keep it as far as possible from any strong magnetic or
electrical fields or any electrical device that may generate high-frequency fields. Set the unit up
in an area that is free of dust, corrosive gases and strong vibrations.
3. Remove any obstructions or materials that could hinder the flow of air under and around the
instrument.
4. Turn on the instrument and allow it to warm up for 15 minutes before taking any readings.
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Operational Panel
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Description of Key Functions
【CLEAR/DEL】
Clear or delete
【SET λ】 Set wavelength
【0Abs/100%T】 Blank (
【LOAD】 Load saved curve;
Set 0Abs and 100%T) or establish baseline;
【MODE】 Select type of measurement;
【ESC】 Escape or back to previous screen;
【ENTER】 Confirm;
【PRINT】 Print test data
【∧】 Scroll up
【∨】 Scroll down
【 -/. 】 Minus/Dot
2. Operation Instruction:
2.1 Preparation and Initialization
Turn on the spectrophotometer by pressing the Power Switch (IO) on the back of the instrument.
The instrument will automatically run a self-initialization check. The screen displays
sequentially the checking status.
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(You may press EXIT to skip 15 minutes warm up which is not recommended).
Initializing
Booting System:
Warm up 15 min....√
System calibration.
Please Select :
After 15 minutes warm up you need to choose either to run full System Calibration or not. If you
choose No, the instrument will use the previously saved calibration data and the display will
move to the main menu and ready to use. If you select Yes, the instrument will go through
system calibration. Below are some displays showing system calibration process.
(Note: If previously saved data is lost the instrument will automatically run system calibration)
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No
The instrument is ready for use. Below is the Main Menu.
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2.2 BASIC MODE ━ %T/Abs or Energy Measurement
2.2.1 Use arrow button to highlight BASIC MODE and then press ENTER to select
Basic mode.
2.2.2 Use【MODE】button to select type of test（%T/Abs or Energy）.
If Energy type is selected the display will show the energy counts as shown below.
2.2.3 To reset wavelength press【SETλ】button. The display first shows the current
wavelength.
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Enter the desired wavelength as shown below.
Press【ENTER】 button to confirm.The instrument will go from previous wavelength
(546nm) to the desired wavelength (500nm) and automatically blank.
Note: You must blank your reference before measure any sample. Follow your lab
procedures for preparing the reference liquid and the steps below to set the blank:
2.2.4 Make a blank reference solution by filling a clean cuvette with distilled or de-
ionized water or other specified solvent. Wipe the cuvette with tissue to remove the
fingerprints and droplets of liquid.
2.2.5 Fit the blank cuvette into the 4-cell holder and place the cuvette in the slot nearest
you. Push the rod so that the cuvette is in the light path. Close the lid.
2.2.6 Set 0.000A or 100%T by pressing 0A/100%T button.
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Energy low! 546nm
Blanking
Note: If “Energy low!” is displayed it might indicate the reference is too dark or
the light beam energy from the lamp is too weak.
2.2.7 Now it is ready to measure your samples:
2.2.8 Remove the blank cuvette if you are testing more than 3 samples. Set it aside in
the case that you may need to reset 0A/100%T later (i.e. change wavelength).
2.2.9 Rinse a second cuvette (or more) with a small amount of sample solution to be
tested. Fill the cuvette and wipe it.
2.2.10 Put the sample cuvette(s) in the sample compartment. Close the lid.
2.2.11 The current sample test result is displayed on the screen.
2.2.12 Press ENTER to confirm and log the result. Up to 20 test results can be logged.
When21st test result is confirmed the first test result will be automatically removed
from the list.
Basic mode
546nm
0.000A
100.0%T
01: 0.418 02: 0.436
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