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2655-00
Instruction Manual
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Cole-Parmer 2655-00 Instruction Manual

Model 2655-00 Flame Photometer
Operator
Manual
Copyright 2001 Cole Parmer Instrument Company.
All rights reserved.
Model 2655-00 Flame Photometer
Intended Use
The Model 2655-00 is a single channel, low temperature flame photometer for the determination of Na, K and Li. This operatorÕs manual contains complete instructions for setting up and using the Model 2655-00. Service information for use by appropriately qualified personnel is also available. The Model 2655-00 is intended for use by persons knowledgeable in safe laboratory practices. If the instrument is not used in accordance with these instructions for use, the protection provided by the equipment may be impaired.
WARNING The Model 2655-00 is designed to be grounded through the power supply lead (line
cord) for safe operation. For the safety of operating personnel and optimum performance make sure that the instrument is only connected to a 3-prong socket (outlet) that has an effective earth connection. If you are in any doubt about the safety of your electrical supply system consult a competent, qualified electrician.
There are no user replaceable parts within the instrument. Do not remove the rear cover from the instrument.
Cole Parmer Instrument Company and its authorized Distributors and Agents consider themselves responsible for the effects of safety, reliability and performance of the Model 2655­00 only if:
¥ Assembly operations, extensions, re-adjustments, modifications or repairs
are only carried out by persons authorized by them.
¥ The electrical installation of the relevant room complies with IEC
requirements or the local regulatory code.
¥ The equipment is used in accordance with the instructions for use.
The information contained in this manual was correct at the time of going to print. However, Cole ParmerÕs policy is one of continuous product improvement and the right to change specifications, equipment and maintenance procedures at any time, without notice, are reserved. This manual is copyrighted, and all rights are reserved. No part of this manual may be reproduced by any means or in any form without prior consent in writing.
Cole Parmer Instrument Company, 635 East Bunker Court, Vernon Hills, IL, 60061, USA
Tel : 847-549-7600 Tol free : 800-323-4340 Fax : 847-247-2929
Model 2655-00 Flame Photometer
Contents
Page
Intended Use.............................................................................................................................. 2
Introduction...............................................................................................................................5
1.1 Introduction....................................................................................................... 5
1.2 Summary of the Test.......................................................................................... 5
1.3 Reagents............................................................................................................. 6
Purification........................................................................................................ 6
Installation ................................................................................................................................7
2.1 Services Required................................................................................................ 7
Electrical Supply................................................................................................ 7
Fuel.................................................................................................................... 7
Air..................................................................................................................... 7
Waste Container................................................................................................ 7
2.2 Site Conditions................................................................................................... 8
2.3 Unpacking.......................................................................................................... 9
Accessory List ................................................................................................... 9
2.4 Assembly............................................................................................................ 9
2.5 Connecting a Chart Recorder............................................................................ 15
Principles of Operation............................................................................................................ 16
3.1 Flame Photometry........................................................................................... 16
Performance Characteristics and Specification......................................................................... 17
4.1 Readout............................................................................................................ 17
4.2 Measurement Ranges........................................................................................ 17
4.3 Specificity........................................................................................................ 17
4.4 Accuracy.......................................................................................................... 17
Linearity.......................................................................................................... 17
Drift ................................................................................................................ 17
Reproducibility................................................................................................. 18
4.5 Warm Up......................................................................................................... 1 8
4.6 Sample Requirement......................................................................................... 18
Type................................................................................................................ 18
Method of presentation ................................................................................... 18
Volume ............................................................................................................ 18
4.7 Chart Recorder Output ..................................................................................... 18
4.8 Environmental Conditions ............................................................................... 1 9
Temperature.................................................................................................... 19
Humidity.......................................................................................................... 19
Installation Category ....................................................................................... 19
4.9 Power Requirements......................................................................................... 19
Voltage ............................................................................................................ 19
Fuses................................................................................................................ 19
Power .............................................................................................................. 19
4.10 Fuel.................................................................................................................. 19
4.11 Air ................................................................................................................... 19
4.12 Size .................................................................................................................. 19
4.13 Weight............................................................................................................. 19
Model 2655-00 Flame Photometer
Contents
Page
Operating Instructions ............................................................................................................. 20
5.1 Front Panel Controls and Indicators (See Fig 5.1)............................................. 20
Power on ......................................................................................................... 20
Flame on.......................................................................................................... 20
Blank............................................................................................................... 20
Sensitivity Fine and Coarse .............................................................................. 20
Fuel.................................................................................................................. 20
Na, K, Li.......................................................................................................... 20
Decimal ........................................................................................................... 20
Power 0/1 ........................................................................................................ 20
5.2 Rear Panel Controls and ConnectorsЙЙЙЙЙЙЙЙЙЙЙЙЙЙЙЙЙЙ..22
Power .............................................................................................................. 23
Data output...................................................................................................... 23
Gas................................................................................................................... 23
Air................................................................................................................... 23
Air regulator .................................................................................................... 23
5.3 Initial Adjustment ............................................................................................ 24
5.4 Operating Instructions...................................................................................... 25
5.5 Shutdown Procedure ......................................................................................... 27
5.6 Operating Hints................................................................................................ 27
Operational Precautions and Limitations ................................................................................. 28
6.1 General............................................................................................................. 28
6.2 Hazards ............................................................................................................ 28
Maintenance............................................................................................................................ 29
7.1 General............................................................................................................. 29
7.2 Daily Maintenance........................................................................................... 29
7.3 Weekly Maintenance ....................................................................................... 29
7.4 Monthly Maintenance...................................................................................... 29
7.5 Six-Monthly Maintenance................................................................................ 30
7.6 Nebuliser .......................................................................................................... 30
Operational Check........................................................................................... 30
Cleaning the Nebuliser...................................................................................... 30
7.7 Cleaning the Mixing Chamber, Burner and Drain.............................................. 32
7.8 Cleaning the Optical Filters and Glass Chimney................................................ 33
7.9 Voltage Selection and Fuse Replacement........................................................... 35
7.10 Deproteinising or Disinfecting Procedure ......................................................... 36
Troubleshooting....................................................................................................................... 37
8.1 Power on LED not illuminated......................................................................... 37
8.2 Flame on LED not illuminated ......................................................................... 37
General ............................................................................................................ 37
Air Supply........................................................................................................ 37
Fuel Supply ...................................................................................................... 37
8.3 Unable to set display to zero ............................................................................ 38
8.4 Unable to set display to standard reading .......................................................... 38
8.5 Unstable results ................................................................................................ 38
8.6 Non-linear results............................................................................................. 39
Spares and Accessories ............................................................................................................. 40
9.1 Ordering Information....................................................................................... 40
9.2 Spares and Accessories...................................................................................... 40
9.3 Standard Solutions ............................................................................................ 41
Appendix A ............................................................................................................................. 42
1
1.1 Introduction
The Cole Parmer Model 2655-00 is a single channel, low temperature flame photometer for the determination of Na, K and Li in clinical samples. Information on Ca and Ba determinations is also available from your Cole Parmer distributor. The Model 2655-00 utilises a digital display and is designed for reliable, trouble-free operation and ease of maintenance. An automatic sample dilutor, Model 805 is available.
The Model 2655-00 has a fail-safe device, which automatically stops the gas flow if the flame does not ignite, or if during operation, the flame is extinguished. Plus an air pressure switch so that if the air pressure falls below a specified value the flame will not ignite or will be extinguished.
1.2 Summary of the Test
Model 2655-00 Flame Photometer
Introduction
NOTE References are listed in Appendix A.
Prior to the advent of flame photometry, Sodium and Potassium were typically determined gravimetrically after the precipitation of relatively insoluble salts such as Sodium Uranyl Zinc Acetate (Ref. 1) and Potassium Chloroplatinate (Ref. 2). As with all chemical methods for these two elements there were cross interferences and also interference from other ions such as NH4+. Many analytical steps such as protein precipitation or ashing of the sample were involved with all the attendant losses and inaccuracies and the complete procedures required many hours.
In many applications, rapid availability of results is of prime importance. By flame photometry both sodium and potassium results on a single sample can be available in less than 5 minutes of the sample reaching the laboratory. (The same is true of lithium results, a determination that was never carried out before the advent of flame photometry). A simple dilution step is all that is required; therefore sample handling, losses and inaccuracies are at a minimum (Ref. 3-10)
Introduction continued
1.3 Reagents
Cole Parmer supply a wide range of reagents, including standards, diluent and maintenance solutions, for use with the Model 2655-00 Flame Photometer. Please refer to Section 9.3 for a complete list of the reagents available.
Dilutions
Samples and standards must be diluted with the same batch of diluent, made up of 1 part Diluent Concentrate to 999 parts deionised or good quality distilled water. The same batch of diluent should be used to zero the instrument and to prepare dilutions of standards and samples. This will prevent variations in water purity affecting the measurements. Great care should be taken so that contamination does not occur when preparing the samples and standards. Remember that the accuracy of the instrument is dependent on the accuracy and purity of the standards used for calibration.
Storage
All solutions should be stored away from direct sunlight, in a cool place (below +25¡C/+77¡F), in an airtight container to prevent evaporation and discolouration. Glass containers should not be used, as they can affect Na concentration levels. Prolonged exposure to the atmosphere must be avoided to prevent evaporation of standard solutions, which could affect concentration.
Model 2655-00 Flame Photometer
Purification
No purification is required for Cole Parmer standard solutions.
2
2.1 Services Required
Electrical Supply
An a.c. supply at 100V, 120V, 220V or 240V ±10%, at 50 or 60Hz, is required for the Model 2655-00. The instrument operating voltage is shown on the voltage selector on the rear panel.
CAUTION If the voltage setting is not correct for the local supply, do not attempt to use
the instrument until it is correctly adjusted as detailed in Section 2.4, paragraph 23.
Fuel
A supply of Propane, Butane or Propane/Butane mixture regulated at the cylinder to
2.1kg/cm_ (30psig), flow rate at least 0.4 litres per minute. The use of industrial quality
gas is not recommended as impurities can enter the delicate gas regulators and can leave deposits of oil and dirt, which will render the instrument inoperable.
Model 2655-00 Flame Photometer
Installation
Natural gas at 3 to 10 inches water gauge, regulated to 2 inches water gauge, can also be used. Natural gas and camping gas regulators are available as optional accessories, refer to Section 9.2.
NB The positioning of Gas cylinders should conform to National and local regulations.
Air
A supply of clean, dry, oil-free air at 1kg/cm_ (14psig), flow rate 6 litres per minute. A suitable air compressor is listed in Section 9.2.
Waste Container
A sink or waste container sited to the right of the instrument will ensure the minimum length of waste tubing. Do not use a waste container with high sides, as this will cause the drain tube to be lifted above the level of the constant head drain.
Installation continued
2.2 Site Conditions
WARNING Under no circumstances install the instrument beneath overhanging
cupboards. There must be at least 1 metre of clear space above the chimney. For optimum performance, this instrument should be installed in accordance with the following conditions: -
1. The environment must be clean and free from dust.
2. The instrument must be placed on a strong, level worktop, free from vibration. The
Model 2655-00 requires approximately 500mm x 500mm of bench space, which includes an area in front for solutions and clearance at the rear for fuel and air tubing, with clear access to the mains supply switch.
3. Avoid sites that expose the instrument to direct sunlight or draughts.
4. To meet the specification the ambient temperature must be within the range +10¡C
to +35¡C and the maximum relative humidity must not be more than 85%, non­condensing.
5. Make sure that the correct voltage is selected for the local a.c. supply, as shown in
the following table. Do not attempt to connect the power supply lead until the voltage is correctly set.
Model 2655-00 Flame Photometer
VOLTAGE SELECTED VOLTAGE RANGE
100V 90V to 110V 120V 100V to 132V 220V 198V to 229V 240V 230V to 264V
Installation continued
2.3 Unpacking
1. Unpack the instrument and accessories. NOTE The Model 2655-00 weighs 9.5kg, follow safe lifting techniques.
2. Check all items for damage.
3. Check that all the items on the Accessory List have been delivered. Contact your
Cole Parmer distributor if you have any problems.
4. The Model 2655-00 is shipped with the following items:
Accessory List
CAT. NO. ITEM QUANTITY
001 72 043 Air tubing, nylon reinforced 2 metres 001 72 114 Fuel tubing 2 metres 400 22 002 Drain tube, 200 mm, and spare tube 200mm 1 pack 100 594 Fuse, 200 mA slo-blo, spares 2 471 71 900 Supply lead, a.c. 1 100 99 010 Nebuliser cleaning wire, pack of 3 1 pack 001 31 076 ÔOÕ ring, spare for mixing chamber 1 400 27 001 Nebuliser 1 400 22 003 Nebuliser inlet tube, polythene, 150mm 1 410 91 001 Operators Manual, English 1 001 08 718 ÔUnexÕ tubing clamp 4 001 56 120 Flame Photometer Standard, 1000ppm Na, 1 bottle, 500 ml 001 56 121 Flame Photometer Standard, 1000ppm K, 1 bottle, 500 ml 001 56 122 Flame Photometer Standard, 1000ppm Li, 1 bottle, 500 ml
Model 2655-00 Flame Photometer
2.4 Assembly
EQUIPMENT REQUIRED: -
ÔPozidrivÕ (cross head) screwdriver 1PT; Flat blade screwdriver.
To assemble the Model 2655-00, proceed as follows:
1. Lift off the chimney cap from the chimney assembly and lift out the glass
chimney. Remove any packing material from inside, making sure that there are no small pieces left within the chimney.
2. Replace the glass chimney with the clear strip at the bottom and refit the chimney
cap on top of the chimney.
3. Remove the nebuliser from its box and screw it to the connector on the end of
the air tubing (item 3, Figure 2.1). Fit the nebuliser to the mixing chamber and position the retainer to lock it into position (item 2, Figure 2.1). Fit the nebuliser
Installation continued
2.4 Assembly continued
Model 2655-00 Flame Photometer
Figure 2.1 Mixing Chamber
1. Nebuliser. 2. Nebuliser retainer. 3. Air tubing. 4. Constant head drain.
5. Fuel restrictor. 6. Fuel tubing.
4. If the instrument is to be used on Natural gas, proceed with paragraph 11. If
Propane or Butane is to be used continue with paragraph 5.
5. Connect the length of rubberised fuel tubing, (001 72 114) supplied, between the
inlet connector on the Propane regulator, (item 5, Figure 2.2) and the cylinder regulator outlet connector. Secure with ÕUnexÕ tubing clamps, supplied. Ensure that the connector at the regulator inlet is tightened to 12nm torque. Continue with paragraph 15.
NB The connection at the cylinder end of the tube must comply with
National regulations.
6. Remove the fuel restrictor (item 5, Figure 2.1) and connect the fuel tubing (item
6, Figure 2.1) directly to the mixing chamber connection.
7. Unscrew and remove the gas inlet, regulator inlet and regulator outlet connectors
(items 4, 5 and 6, Figure 2.2) and remove the copper tubing (item 2, Figure 2.2).
8. Connect the regulator inlet, with connector, (item 5, Figure 2.2) to the gas inlet
(item 4, Figure 2.2).
Installation continued
2.4 Assembly continued
Model 2655-00 Flame Photometer
Figure 2.2 Rear Panel
1. Natural gas regulator assembly (see Figure 2.3). 2. Copper tubing.
3. Air regulator adjuster. 4. Gas inlet connector. 5. Regulator inlet connector.
6. Regulator outlet connector.
9. Locate the Natural gas regulator and mounting bracket ordered as optional
accessories and packed separately from the instrument. Fit the mounting bracket to the rear panel with the two screws supplied and slot in the Natural gas regulator, Figure 2.3. Make sure that the gas flow arrow on the regulator points to the right.
10. Connect the Natural gas supply to the Natural gas regulator inlet with the fuel
tubing and ÔUnexÕ clamp supplied with the instrument.
Installation continued
2.4 Assembly continued
11. If it is necessary to fit the filter because of suspected dirty gas then this should
be fitted before the Natural Gas regulator by cutting the rubber hose and fitting the filter in the tubing with the arrow pointing towards the regulator. Secure the tubing to the filter and regulator with the extra Unex clips supplied with the Natural Gas regulator kit.
12. Connect the Natural gas outlet tubing (supplied with the regulator) to the gas
inlet connector (item 4, Figure 2.2), using the connector and nipple removed as described in paragraph 7 above bypassing the Propane/Butane regulator. Secure with ÔUnexÕ clamp supplied.
Model 2655-00 Flame Photometer
Figure 2.3 Natural Gas Regulator
1. Regulator screw cap. 2. Filter in line with Rubber hose supplied with the instrument. 3. Natural gas outlet tubing. 4. Mounting bracket.
13. Remove the screw cap (item 1, Figure.2.3). Rotate the screw head fully
clockwise, and then counterclockwise exactly one turn.
14. Refit screw cap.
15. Turn on the fuel supply and check all connectors for leaks, using soap solution.
Do not use the instrument until you are satisfied that the installation is leakproof.
16. Connect the length of reinforced Nylon hose, supplied, between the air
compressor outlet and the air inlet connector on the rear panel. Secure both ends with ÔUnexÕ tubing clamps, supplied.
Installation continued
2.4 Assembly continued
17. Fit one of the 200mm lengths of drain tubing (item 4, Figure 2.4) to the outlet on the constant head drain (or Gas Trap). If necessary connect a suitable length of tubing (not supplied), to extend the drain tubing to carry waste to a sink or other drain receptacle. The downward flow of waste must not be restricted. The second length of tubing supplied is a spare for either the ÔUÕ tube or drain tube.
Model 2655-00 Flame Photometer
Figure 2.4 Filling the ÔUÕ Tube
1. Wash bottle. 2. Constant head drain (Gas Trap). 3. ÔUÕ tube. 4. Drain tube.
5. Spring clip.
18. Make sure that the constant head drain (Gas Trap) is positioned so that the lip at the top is resting on the spring clip. Use a wash bottle (item 1, Figure 2.4) to fill the ÔUÕ tube with deionised water. Sufficient water should be used to purge the tube of air. Allow excess water to flow back into the drain.
IMPORTANT Do not continue until you are satisfied that the ÔUÕ tube has been
completely filled with water, and is purged of air.
Installation continued
2.4 Assembly continued
19. The instrument is supplied with Na, K and Li filters fitted. To replace any of these filters, refer to Section 7.8.
Model 2655-00 Flame Photometer
Figure 2.5 Voltage Selector
1. Voltage selector. 2. Fuse holders.
20. Check that the voltage shown on the voltage selector is correct for the local supply. If it is not, open the voltage selector compartment. Slide the voltage selector bobbin, item 1, Figure 2.5 out of the instrument and replace it so that the required voltage setting will be visible through the window of the compartment cover. Close the compartment cover.
CAUTION Do not attempt to rotate the voltage selector bobbin when it is fitted
in the voltage selector, as this will damage the contacts.
21. Fit a suitable 3-pin plug to the a.c. supply lead. Check that the correct colour coded leads are connected to the plug terminals. Follow the plug manufacturerÕs fitting instructions.
CAUTION Ensure that the green/yellow earth connector in the a.c. supply lead
is connected to a properly grounded earth point, the brown lead is connected to the Line terminal (L) and blue lead is connected to the Neutral terminal (N).
22. If necessary, set the power switch (below the chimney) to the 0 position. Connect the a.c. supply lead to the power receptacle on the rear panel and connect the plug to a convenient supply socket.
23. If a chart recorder is to be used with the instrument continue with Section 2.5. If not, continue with Section 5, Operating Instructions.
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