Cole-Parmer 2100, 2100UV Instruction Manual

Cole-Parmer

2100/2100UV SERIES

SPECTROPHOTOMETER

USER’S MANUAL

Cole Parmer Instruments Company

625 East Bunker Court

Vernon Hills, IL 60061

800-323-4340

Table of Contents

General Information…………………………………………………………………………………….…….... 3

Introduction………………………….………………………................................................................................. 4

Working Principle………………………………..………………………………………………………..….…..... 4

Specifications........................…………………..…………...………………………………………………………. 5

Unpacking Instructions………………………..……………………………………………………………..... 5

Installation………………………………………………..……………………………………………………………..... 6

2100 and 2100UV Spectrophotometer Operation Panel………………………...... 6

Model 2100UV Operation Panel……………………………………………………........... 6

Model 2100 Operation Panel………………….……………………………………………. 9 Start-up Self Calibration…………………………………………………………………………………….… 10

Changing Curette Holders……………………………………………..……….…………….………….... 13

Basic Operation…………………………………………………………………………………………………….... 14

Sample Preparation and Analysis………………………………………………………... 14 Concentration Mode Factor Mode…………………………………………………………………………………………... 15

Output and Data Processing…………………………………………………………………………….. 16

Printer

…………………………………………………………………………………………………..... 16

Application Software……………………………………………………..………………….. 16

Maintenance………………………………………………………………………………………………………….. 17

Lamp Replacement Wavelength Calibration Check…………………………………………………………... 18 Absorbance Accuracy Check…………………………………………………………….. 19 Stray Light Check…………………………………………………………………………………... 20

2100 and 2100UV Parts List………………………………………………………………………….……..... 21

Troubleshooting………………………………………………………………….…………………….……….……. 23 Error Codes……………………………………………………………………………………………………………… 25 Attachment……………………………………………………………………………………………………………… 26

…………………………………………………………………………….. 15

………………………………………………………………………..….... 17

General Information

The spectrophotometer described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory. For the correct and safe use of this spectrophotometer it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual.

The inside of the power supply unit is a hazardous area and its cover should not be removed under any circumstances. ANY Servicing must be done by an authorized person.

Some of the chemicals you use in the spectrophotometer may be corrosive, flammable, radioactive, toxic, and/or potentially infective. Care should be taken to follow the normal laboratory procedures for handling chemicals and samples. Please carefully read the

Safety, Electrical, Warning, Performance and Radio Interference instructions below. Safety

This spectrophotometer has been designed and tested in accordance with EN 61326-1: 1997 Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use standard (EMC Requirements). The spectrophotometer has been supplied in a safe condition.

The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT, 1974.

Read the following before installing and using the instrument and its accessories.

Electrical

Before switching on the spectrophotometer, make sure it is set to the voltage of the local power supply (see Installation).

The main plug shall be inserted in a socket provided with a protective earth contact. The protective action must not be negated by the use of an extension cord without a protective conductor.

Warning

Any interruption of the protective conductor inside or outside the spectrophotometer or disconnection of the protective earth terminal is likely to make the spectrophotometer dangerous. Intentional interruption is prohibited. has been impaired, the spectrophotometer shall be made inoperative and be secured against any unintended operation. NEVER touch or handle the power supply on the Model 2100UV due to the high voltage.

The protection is likely to be impaired if, for example, the apparatus

• Shows visible damage

• Fails to perform the intended measurements

• Has been subjected to prolonged storage under unfavorable conditions

Whenever it is likely that the protection

• Has been subjected to severe transport stresses

Performance

Carry out performance checks with particular reference to wavelength and absorbance accuracy to ensure that the spectrophotometer is working within its specification, especially when making measurements of an important nature. Performance checks are detailed in this manual.

Radio Interference

For compliance with the EMC standards referred to in the EC Declaration of Conformity, it is necessary that only shielded cables supplied by are used when connecting the instrument to computers and accessories.

Introduction

The Model’s 2100 and 2100UV Spectrophotometers are single beam, general purpose instruments designed to meet the needs of the conventional laboratory. 2100 and 2100UV are ideal for various applications, such as: Clinical Chemistry, Biochemistry, Petrochemistry, Environmental Protection, Food and Beverage Labs, Water and Waste Water Labs and other fields of quality control.

Featuring a digital display of photometric result, easy operation and wavelength range of 325~1000 nm (Model 2100) or 200 ideal for measurements in the visible wavelength region of the electromagnetic spectrum (ultraviolet and visible for the Model 2100UV).

~1000 nm (Model 2100UV), 2100 and 2100UV are

Working Principle

The spectrophotometer consists of five parts:

1) Halogen/Deuterium Lamp to supply the light;

2) A Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation;

3) A Sample Compartment to accommodate the sample solution;

4) A Detector to receive and convert the transmitted light to an electrical signal;

5) A Digital Display to show absorbance or transmittance. Figure-1 illustrates the relationship between these

Light Source Monochromator Sample Compartment Detector Digital Display

In your spectrophotometer, light from the lamp is focused on the entrance slit of the monochromator where the collimating mirror directs the beam onto the grating. The grating

parts.

Figure-1 Block Diagram for the Spectrophotometer

100%T 0 A

disperses the light beam to produce the spectrum, a portion of which is focused on the exit slit of the monochromator by a collimating mirror. From here the beam is passed to a sample compartment through one of the filters, which helps to eliminate unwanted second order radiation from the diffraction grating. Upon leaving the sample compartment, the beam is passed to the silicon photodiode detector and causes the detector to produce an electrical signal that is displayed on the digital display.

Specifications

Table-1 lists the specification for both Model 2100 and 2100UV.

Wavelength Range 325~1000 nm 200~1000 nm Spectral Bandpass 5 nm Wavelength Accuracy Better than 2 nm Wavelength Repeatability Better than 1 nm Stray Radiant Energy < 0.3% T at 340 and 400 nm Photometric Range 0~125%T

Photometric Accuracy Power Requirements Sample Holder 4-poisiton Cuvette Holder Dimensions 470W x 400D x 140H (mm) Net Weight 10 kg (22 lbs) 12 kg (27 lbs)

Table-1 Specifications

Model 2100 Model 2100UV

-0.1~2.5 Abs

0~1999 C (0~1999 Factor)

±0.004 Abs at 0.5 Abs

115/230 V.

±10%, 60/50 Hz switchable

Unpacking Instructions

Carefully unpack the contents and check the materials against the following packing list to ensure that you have received everything in good condition:

Packing List

Description

Spectrophotometer Dust Cover Cuvette

Table-2 Packing List

Quantity

Model 2100 Model 2100V

1 1 1 1

Set of 4, glass Set of 2, quartz; Set of 4, glass

4-position Cuvette Holder (Manual) Power Cord

User Manual

1 (installed) 1 (installed)

1 1 1 1

Installation

1. After carefully unpacking the contents, check the materials with the packing list above to ensure that you have received everything in good condition.

2. Place the instrument in a suitable location away from direct sunlight. In order to have the best performance from your instrument, keep it as far as possible from any strong magnetic or electrical fields or any electrical device that may generate high-frequency fields. Set the unit up in an area that is free of dust, corrosive gases and strong vibrations.

3. Remove any obstructions or materials that could hinder the flow of air under and around the instrument.

4. Select either 230V or 125V/115V on the voltage selector switch on the back of the 2100 and 2100UV shown in Figure-2, to match your local main voltage supply.

5. Turn on your 2100 and 2100UV and allow it to warm up for 15 minutes before taking any readings.

Figure-2 Back of 2100 and 2100UV

2100 and 2100UV Spectrophotometer Operation Panel

Model 2100UV Operation Panel Figure-3 shows the Model 2100UV and its Components:

• Sample Compartment: Accommodate different cuvette holders. 4-position Cuvette

Holder is preinstalled in the standard package

•

Power Indicator: Indicate whether power is on or off

•

Cuvette Holder Control Knob: Select the sample position of the 4-position Cuvette

Holder by pulling out or pushing in

Operation Panel: has eleven components, see below for details.

•

Figure-4 illustrated the detailed information of the Operation Panel:

1. Data Display Screen: Display the data of Transmittance, Absorbance, Concentr ation, and Factor modes.

2. Mode Indicator: The red LED light indicates the operation MODE. Allow the operator to

know the measurement mode currently in use (T--%Transmittance, A--Absorbance, C-Concentration, and F--Factor).

3. 0A/100%T Button: Blank the 2100/2100UV when blank reference solution is in the Sample Compartment. Every time a new wavelength is selected, press 0A/100%T Button to confirm. It will automatically blank the 2100/2100UV.

4. MODE Button: There are four modes. T mode is transmittance mode; A mode is absorbance mode; C mode is to measure unknown sample concentra tion through a standard solution; F mode is to measure unknown value with a previously determined factor.

5. PRINT Button: Press to send Data Display Screen and Wavelength Display Screen results to RS-232C port and printer.

6. Wavelengt h Display Screen: Display the current/desired wavelength.

7. Wavelengt h Control Buttons: Allow user to select the desired wavelength with the ∧ and

∨

(increase and decrease) buttons.

8. INC/DEC ( Concentration/Factor Buttons) and Lamp Control Buttons: Allow user to

increase or decrease the displayed Concentration at C mode or Factor number at F mode. At T mode or A mode, press INC button will shutdown the halogen lamp (W), press DEC mode will shutdown the deuterium lamp (D2). To turn on the lamps, just press the INC/DEC button again.

9. ENT Button: First press at C mode enters reading on Data Display Screen. Other presses send displayed results to printer. If using Windows-based Application So ftware, press this button to communicate with the software (refer to the 2100 Series

Spectrophotometer Software User’s Manual Version SB-2.10 for details). If operating in F Mode, then press to enter factor. This causes the Mode to enter the factor number and change to C mode.

10. Visible Lamp Indicator (W): In the operating mode of Absorbance or %Transmittance (A or T), pressing the INC button will turn off/on the lamp.

11. UV Lamp Indicator (D2): In the operating mode of Absorbance or %Transmittance (A or T), pressing the DEC button will turn off/on the lamp. Since Deuterium lamp (D2) is an

expensive item, it is recommended to turn off the) when not using ultraviolet light for measurement.

Figure-3 Model 2100UV Spectrophotometer

Figure-4 Operation Panel of Model 2100UV

Model 2100 Operation Panel

The Model 2100 has the similar outlook as Model 2100UV. It also has the following components:

• Sample Compartment: Accommodate different cuvette holders. 4-position Cuvette

Holder is preinstalled in the standard package

•

Power Indicator: Indicate whether power is on or off

•

Cuvette Holder Control Knob: Select the sample position of the 4-position Cuvette

Holder by pulling out or pushing in

Operation Panel: has nine components, see below for details.

•

Figure-5 illustrated the detailed information of the Operation Panel:

1. Data Display Screen: Display the data of Transmittance, Absorbance, Concentration, and Factor modes.

2. Mode Indicator: The red LED light indicates the operation MODE. Allow the operator to

know the measurement mode currently in use (T--%Transmittance, A--Absorbance, C-Concentration, and F--Factor).

5. PRINT Button: Press to send Data Display Screen and Wavelength Display Screen results to RS-232C port and printer.

6. Wavelength Display Screen: Display the current/desired wavelength.

7. Wavelengt h Control Buttons: Allow user to select the desired wavelength with the

∧

and ∨ (increase and decrease) buttons.

8. INC/DEC (Concentration/Factor Buttons): Allo w user to increase or decrease the displayed Concentration at C mode or Factor number at F mode.

Spectrophotometer Software User’s Manual Version SB-2.10 for details). If operating in F Mode, then press to enter factor. This causes the Mode to enter the factor number and change to C mode.

Figure-5 Operation Panel of Model 2100

Start-up Self-Calibration

The Model 2100 and 2100UV have self-calibration programs that begin when the instrument is powered on. The following are the steps taken automatically each time the instrument is switched on. Each step is displayed on the

Figure-6 Calibration: P1 Process, P2 Process, P3 Process, P4 Process, Show Process, PC CONN Process, and Normal Start-up Screen.

to Figure 12 and Table-3 show the detailed information of the Start-up Self-

Wavelength Display Screen.

Figure-6 P 1 Process

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