Cole-Parmer 02655-10, 02655-15 Operator Manual
Model 02655-10
And
Model 02655-15
Flame Photometer
Operator Manual
Cole-Parmer Instrument Company
625 East Bunker Court
Vernon Hills
IL 60061
USA
425 91 002 Issue 9, 6th August 2008 ECN 527
G:\DOCUMENT CONTROL\Manuals\Flame\425\42591002 Cole Parmer M420 & M425 Operator Manual Issue 9.doc
Model 02655-10 & 02655-15 Flame Photometer
Short Form Instructions Model 02655-10/15 Flame Photometer
The Default mode for the Model 02655-10/15 that will appear when the instrument is
switched on is: Dual channel Sodium and Potassium with Peak detection and Reference mode
selected. These instructions are to be used in the default mode.
NB In Reference Mode the Blank solution as well as the Calibration Standard
and Sample must contain 100ppm Li (Use the Blank solution to dilute the
Standards and Samples).
Operation: -
Set Up and Ignition
1. Ensure that the ‘U’ tube is full of deionised water.
2. Connect the gas supply (maximum 30psi Propane or Butane) via a Primary
regulator.
3. Switch on the compressor and regulate the air pressure to 11psi on the gauge
at the rear of the instrument.
4. Switch on the instrument with the “1/O” switch.
5. If the flame ignites then an auto sequence will test various circuits and end with
the ‘000’ flashing in both channel displays.
6. If the flame does not ignite, switch off, wait 15 - 20 seconds and repeat step 4.
It may take 3 or 4 attempts if the instrument has not been used for some time.
Blanking (Zeroing)
7. With the ‘000’ flashing aspirate the blank solution and press the ‘Blank’ button
once.
8. The instrument will then zero to the blank values; this will take at least 30
seconds; Remove the blank when complete.
Calibration
9. When the blanking procedure is complete, the previous calibration values that
were used will appear in the displays and the ‘Cal’ indicators will flash. If you
wish to calibrate to these values go to 11.
10. If you wish to change the values use the ‘+ and –’ keys before aspirating the
standard solution.
11. Then press the Sodium ‘Set’ button, then start aspirating the standard and
press the Potassium ‘Set’ button within a few seconds.
12. Once the instrument has calibrated itself then the ‘Cal’ lights will flash; remove
the standard and press ‘Set’ on both channels again.
Measuring a Sample
13. After 5 seconds both displays will flash and the “Measure” light will illuminate,
the first sample can now be measured.
14. Aspirate the sample and then immediately press the ‘Print/Measure’ button.
15. The instrument will measure the sample until the peak algorithm captures the
value and the measure light shows continuously. Remove the sample.
16. After 5 seconds the displays will flash and the next measurement can be made.
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Table of Contents
Page
Short Form Instructions Model 02655-10/15 Flame Photometer .................................................. 2
Operation: - ................................................................................................................................. 2
1 INTRODUCTION ........................................................................................................... 6
1.1 Contacting Cole-Parmer ...................................................................................... 6
1.2 Using this manual ............................................................................................... 6
1.3 Safety Precautions ............................................................................................... 7
1.4 Reagents ............................................................................................................. 8
Reference/Lithium Standard ................................................................................ 8
Blank Storage...................................................................................................... 8
Purification ......................................................................................................... 8
1.5 Dilutions ............................................................................................................. 8
Dilution Ratio ..................................................................................................... 9
Urine Samples ..................................................................................................... 9
2 INSTALLATION .......................................................................................................... 10
2.1 Location ............................................................................................................ 10
2.2 Services Required .............................................................................................. 10
Electrical Supply ............................................................................................... 10
Fuel ................................................................................................................... 10
Air .................................................................................................................... 10
Waste Container ................................................................................................ 10
2.3 Unpacking......................................................................................................... 11
Accessory List ................................................................................................... 11
2.4 Assembly .......................................................................................................... 12
2.5 Connecting Peripheral Devices .......................................................................... 16
Printer ............................................................................................................... 16
Computer .......................................................................................................... 16
Analogue Devices.............................................................................................. 16
Auto-sampler .................................................................................................... 16
2.6 Setting Date and Time....................................................................................... 17
2.7 Setting Filter Factors ......................................................................................... 18
2.8 Setting Units ..................................................................................................... 18
2.9 Setting Sample Delay (in Peak mode) ................................................................ 19
2.10 Setting the Output Voltage ................................................................................ 19
2.11 Setting the analogue damping coefficient. ......................................................... 19
3 PRINCIPLES OF OPERATION ..................................................................................... 20
3.1 Application of Flame Photometry ...................................................................... 20
3.2 Principles of Flame Photometery ....................................................................... 20
3.3 The Internal Standard Mode .............................................................................. 21
4 PERFORMANCE CHARACTERISTICS & SPECIFICATIONS ................................... 22
4.1 Readout ............................................................................................................. 22
4.2 Measurement Ranges ........................................................................................ 22
4.3 Specificity ......................................................................................................... 23
4.4 Accuracy ........................................................................................................... 23
Linearity ........................................................................................................... 23
Drift .................................................................................................................. 23
Reproducibility (using Peak mode) .................................................................... 23
Using Peak with Ref (Internal Standard) Mode.................................................. 23
4.5 Warm Up .......................................................................................................... 24
4.6 Sample Requirements ........................................................................................ 24
Type .................................................................................................................. 24
Method of Presentation...................................................................................... 24
Volume ............................................................................................................. 24
4.7 Chart Recorder Output ...................................................................................... 25
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4.8 Environmental Conditions................................................................................. 25
Temperature ...................................................................................................... 25
Humidity ........................................................................................................... 25
4.9 Power Requirements .......................................................................................... 25
Voltage ............................................................................................................. 25
Power ................................................................................................................ 25
4.10 Fuel ................................................................................................................... 25
4.11 Air .................................................................................................................... 26
4.12 Size ................................................................................................................... 26
4.13 Weight .............................................................................................................. 26
5 DESCRIPTION OF INSTRUMENT .............................................................................. 27
5.1 Front Panel Controls and Indicators .................................................................. 27
Primary Function .............................................................................................. 27
Secondary Function ........................................................................................... 27
Tertiary Function .............................................................................................. 27
Quaternary Function ......................................................................................... 27
Blank ................................................................................................................ 27
Channel selector ................................................................................................ 27
Mode Selector ................................................................................................... 27
Measure/Print.................................................................................................... 27
Channel 1 Set .................................................................................................... 27
Channel 2 Set .................................................................................................... 27
5.1.1 Controls ............................................................................................................ 27
‘-’ ‘Set’ ‘+’ ..................................................................................................... 27
(Feedback beep confirms the action) .................................................................. 27
Blank ................................................................................................................ 28
Single/Dual and Element Selection ................................................................... 28
Peak/Ref/Cont ................................................................................................... 28
Measure ............................................................................................................ 29
5.1.2 Description of Indicators ................................................................................... 29
Cal (Ch1 & Ch2) ............................................................................................... 29
Single................................................................................................................ 29
Dual .................................................................................................................. 29
Peak .................................................................................................................. 29
Ref .................................................................................................................... 29
Cont .................................................................................................................. 30
Measure ............................................................................................................ 30
Flame On .......................................................................................................... 30
Power On .......................................................................................................... 30
5.2 Rear Panel Controls and Connections ................................................................ 30
Channel 1 & 2 Data Outputs ............................................................................. 31
RS232/Printer Output ........................................................................................ 31
External Device Output ..................................................................................... 31
Power ................................................................................................................ 31
Gas ................................................................................................................... 31
Air .................................................................................................................... 31
Air Regulator .................................................................................................... 31
5.3 Operating Modes ............................................................................................... 32
5.3.1 Single/Dual Mode ............................................................................................. 32
Single................................................................................................................ 32
Dual .................................................................................................................. 32
Blanking ........................................................................................................... 32
Calibration ........................................................................................................ 32
5.3.2 Peak/Cont ......................................................................................................... 33
Peak .................................................................................................................. 33
The Peak Cycle ................................................................................................. 33
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5.3.3 Ref Mode .......................................................................................................... 34
Cont .................................................................................................................. 34
5.3.4 Blank ................................................................................................................ 34
6 OPERATING THE INSTRUMENT .............................................................................. 35
6.1 Start Up ............................................................................................................ 35
6.1.1 Compressor Start Up ......................................................................................... 35
6.1.2 Switch On ......................................................................................................... 35
6.1.3 Ignition Successful ............................................................................................ 35
6.2.1 Required Blanking ............................................................................................ 35
6.2.2 Required Calibration ......................................................................................... 36
6.3 Sample Measuring ............................................................................................ 37
6.3.1 Measuring in Peak mode ................................................................................... 37
6.3.2 Measuring in Continuous mode ......................................................................... 37
6.4 Automatic operation .......................................................................................... 37
6.5 Sample Numbering ........................................................................................... 38
6.5.1 Reset of sample number..................................................................................... 38
6.5.2 Repeat sample ................................................................................................... 38
6.5.3 QC sample ........................................................................................................ 38
6.6 Shutdown Procedure.......................................................................................... 38
7 OPERATING HINTS, PRECAUTIONS & LIMITATIONS ........................................... 39
7.1 Hints ................................................................................................................. 39
7.2 Operational Precautions and Limitations ........................................................... 39
7.3 Hazards ............................................................................................................. 40
7.4 Measuring Calcium ........................................................................................... 40
8 CLEANING AND MAINTENANCE PROCEDURES ................................................... 41
8.1 General ............................................................................................................. 41
8.2 Daily Maintenance ............................................................................................ 41
8.3 Weekly Maintenance ......................................................................................... 41
8.4 Monthly Maintenance ....................................................................................... 41
8.5 Six Monthly Maintenance ................................................................................. 42
8.6 Nebuliser ........................................................................................................... 42
8.6.1 Operational Check ............................................................................................ 42
8.6.2 Cleaning the Nebuliser ...................................................................................... 43
8.7 Cleaning the Mixing Chamber, Burner and Drain ............................................. 43
8.7 Cleaning the Mixing Chamber, Burner and Drain continued ............................. 44
8.8 Cleaning the Glass Chimney ............................................................................. 44
8.10 Deproteinising or Disinfecting Procedure .......................................................... 45
9 ACCESSORIES, CONSUMABLES & SPARE PARTS ................................................. 46
9.1 Ordering Information ........................................................................................ 46
9.2 Spares and Accessories ...................................................................................... 46
9.3 Reagents ........................................................................................................... 47
APPENDIX A ........................................................................................................................... 48
BIBLIOGRAPHY ...................................................................................................................... 48
APPENDIX B ........................................................................................................................... 49
APPENDIX C ........................................................................................................................... 51
Cole Parmer Instruments Warranty Statement ........................................................................... 52
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1 Introduction
1.1 Contacting Cole-Parmer
Postal address for letters and orders:
Cole-Parmer Instrument Company
625 East Bunker Court
Vernon Hills
IL 60061
USA
Telephone 847 549 7600
Toll Free 800 323 4340
Fax 847 247 2929
For Certificates of Analysis and Material Safety Data Sheet downloads, contacting Distributors and
Technical and Application information on our products go to our website
www.coleparmer.com
The following section must be carefully read before unpacking, installing, using, maintaining or
repairing the instrument and/or its accessories.
1.2 Using this manual
This manual is structured to allow both quick access to a measurement from the
default position of the Instrument (refer to Section entitled Short Form
Instructions on page 2) and an in depth understanding of the Set-up, Operation
and Maintenance of these two advanced Flame Photometers.
The information contained in this manual was correct at the time of going to print. However, Cole-Parmers’
policy is one of continuous product improvement and the right to change specifications, equipment and
maintenance procedures at any time, without notice, are reserved.
This manual is copyrighted, and all rights are reserved. No part of this manual may be reproduced by any means
or in any form without prior consent in writing.
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Introduction
continued
1.3 Safety Precautions
The Model 02655-10 and 02655-15 are intended for use by persons knowledgeable in
safe laboratory practices. If the instruments are not used in accordance with these
“Instructions for use”, the protection provided by the equipment may be impaired.
WARNING The Model 02655-10/15 are designed to be earthed (grounded) through
the power supply lead (line cord) for safe operation. For the safety of operating
personnel and optimum performance make sure that the instrument is only
connected to a 3-pin socket (outlet) that has an effective earth connection. If you
are in any doubt about the safety of your electrical supply system consult a
competent, qualified electrician.
There are no user replaceable parts within the instrument. Do not remove the rear
cover from the instrument.
Cole-Parmer Instrument Company and its authorised Distributors consider themselves responsible for the effects
of safety, reliability and performance of the Model 02655-10 and 02655-15 only if: ** Assembly operations, extensions, re-adjustments, modifications or repairs are only carried out by
persons authorised by them.
** The electrical installation of the relevant room complies with IEC requirements or the local regulatory
code.
** The equipment is used in accordance with the instructions for use.
PLEASE NOTE that no cables neither for power-in nor for signals-out to the printer, computer via
RS232 output nor analogue to a chart recorder, nor signal cables from an Autosampler nor any other
device should exceed 2.5 metres in length.
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Introduction
continued
1.4 Reagents
Cole-Parmer supply a wide range of reagents, including standards, diluent and
maintenance solutions, for use with the Model 02655-10 and 02655-15 Flame
Photometers. Please refer to Section 9.3 for a complete list of the reagents available.
Reference/Lithium Standard
The full benefit of the technology incorporated in the Model 0655-10/15 is utilised in
the default mode of operation. This mode, ‘Peak’ and ‘Reference’, requires that the
blank, standards and samples contain Lithium at the concentration of approximately
100ppm in the final solution that is aspirated. This is best achieved if the diluent
contains 100ppm Lithium. That is 100ml of the 1000ppm Li standard solution supplied
with the instrument (Part No. 001 56 622) diluted to 1 litre with the diluent described
in the following paragraph.
Blank Storage
All solutions should be stored away from direct sunlight, in a cool place (below
25°C/77°F), in an airtight container to prevent evaporation and discolouration. Glass
containers should not be used, as they can affect Na concentration levels. Prolonged
exposure to the atmosphere must be avoided to prevent evaporation of standard
solutions.
Purification
No purification is required for Cole-Parmer standard solutions.
1.5 Dilutions
Samples and standards should be diluted with the same batch of diluent, made up of 1
part Diluent Concentrate (Part # 001 56 181) to 999 parts good quality distilled water.
The same batch of diluent should be used to zero the Model 02655-10 and Model
02655-15 and to prepare dilutions of standards and samples. This will prevent
variations in water purity affecting the measurements.
Great care should be taken so that contamination does not occur when preparing the
samples and standards. Remember that the accuracy of the instrument is dependent on
the accuracy and purity of the Standards and Blank used for calibration.
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Introduction
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Dilution Ratio
The Model 02655-10/15 are optimised for the following Dilution Ratios: -
In Clinical Mode
Na & K both Serum and Urine: - 1:200 with diluent described above
(including Li if used in Reference Mode).
Li (Measurement) Dilution Ratio: - 1:50.
In Non Clinical Mode
The user should ascertain from his sample what is appropriate by reference to
the input concentrations listed in the specification in Section 4 pp 19/20.
To aid the dilution of clinical samples Cole-Parmer produce a Dilutor, the Model 805,
which automates the process and gives extremely reproducible dilution ratios and
therefore precise results.
Urine Samples
The Potassium content of urine samples is some 20 times that of plasma or serum. In
the past it has been necessary to pre-dilute urine samples prior to following a normal
dilution procedure with e.g. Model 805. The design of the Model 02655-10/15
however, minimises the need for this pre-dilution and urine samples with a Potassium
concentration of 120mmol/l or less can be treated in exactly the same way as plasma
and serum samples provided that the appropriate standard is used to calibrate the
instrument for the high Potassium value. Samples with a Potassium level greater than
120mmol/l should be pre-diluted 1 part in 5 prior to the normal dilution 1 part in 200.
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2 Installation
2.1 Location
WARNING Under no circumstances install the Model 02655-10/15 beneath
overhanging cupboards. There must be at least 1 metre of clear space
above the chimney.
For optimum performance, this instrument should be installed in
accordance with the following conditions: -
2.1.1 The environment must be clean and free from dust.
2.1.2 The instrument must be placed on a strong, level worktop, free from
vibration. The Model 02655-10/15 requires approximately 500mm x
500mm of bench space, which includes an area in front for solutions
and clearance at the rear for fuel and air tubing, with clear access to the
mains supply switch.
2.1.3. Avoid sites that expose the instrument to direct sunlight or draughts.
2.1.4. To meet the specification the ambient temperature must be within the
range +10°C to +35°C and the maximum relative humidity must not be
more than 85%, non-condensing.
2.2 Services Required
Electrical Supply
An A.C. supply between 90V and 240V ±10%, at 50Hz or 60Hz, is required for the
Model 02655-10 and 02655-15. The instrument is powered from an external power
supply, which will accommodate these voltages without any need to adjust the
instrument.
Fuel
A supply of Propane, Butane or Propane/Butane mixture regulated at the cylinder to
2.1kg/cm2 (30psi), flow rate at least 0.4 litres per minute.
(NB. Propane only, gives the best results for Ca on the Model 02655-15).
Primary regulators to fit cylinders are available from Cole-Parmer, regulators are
available as optional accessories, refer to Section 9.2.
Air
A supply of clean, dry, oil-free air at 1kg/cm2 (11psi), flow rate 6 litres per minute. A
suitable air compressor is listed in Section 9.2.
Waste Container
A sink or waste container sited to the right of the instrument will ensure the minimum
length of waste tubing. Do not use a waste container with high sides, as this will cause
the drain tube to be lifted above the level of the constant head drain.
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Installation
continued
2.3 Unpacking
1. Unpack the instrument and accessories.
NOTE The Model 02655-10/15 weighs 9.5kg; follow safe lifting techniques.
2. Check all items for damage.
3. Check that all the items on the Accessory List have been delivered. Contact
your Sherwood Scientific distributor if you have any problems.
4. The Model 02655-10/15 is shipped with the following items: -
Accessory List
CAT. NO. DESCRIPTION QUANTITY
001 72 043 Air tubing, nylon reinforced 2 metres
001 72 114 Fuel tubing 2 metres
400 22 002 Drain tube, 200 mm, and spare tube 200mm 1 pack
001 53 420 Universal external power supply 1
100 99 010 Nebuliser cleaning wire, pack of 3 1 pack
001 31 076 'O' ring, spare for mixing chamber 1
420 08 102 Nebuliser 1
400 22 003 Nebuliser inlet tube, polythene, 150 mm 1
425 91 002 Operators manual
926 09 052 RS232 Output Cable 1
001 08 718 'Unex' tubing clamp 4
001 56 100 Standard Solution containing l40.0mmol/l Na,
5.00mmol/l K and 1.5mmol/l Li
001 56 622 Lithium 1000ppm Standard, 6 x 100ml 1 pack
001 56 620 Sodium 1000ppm Standard, 6 x 100ml 1 pack **
001 56 621 Potassium 1000ppm Standard, 6 x 100ml 1 pack **
001 56 623 Calcium 1000ppm Standard , 6 x 100 ml 1 pack ***
410 92 001 CDROM, Flame Training Guide 1
* supplied with Clinical instruments.
** supplied with Industrial instruments.
*** supplied with Model 02655-15.
1
1 x 100 ml*
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Installation
continued
2.4 Assembly
EQUIPMENT REQUIRED: 'Pozidriv' (cross head) screwdriver 1PT
Flat blade screwdriver.
To assemble the instrument, proceed as follows: -
1. Lift off the chimney cap from the chimney assembly and lift out the glass
chimney. Remove any packing material from inside, making sure that there are
no small pieces left within the chimney.
2. Replace the glass chimney with the clear strip at the bottom and refit the
chimney cap on top of the chimney.
3. Remove the Nebuliser (420 08 102) from its box and push the barbed
connector in to the end of the air tubing (item 3, figure 2.1). Fit the Nebuliser
(item 1, figure 2.1) to the mixing chamber and position the retainer (item 2,
figure 2.1) to lock it into position. Locate the Nebuliser inlet tubing (400 22
003) and fit over the narrow tube in the front of the Nebuliser.
Figure 2.1 Mixing Chamber
1. Nebuliser, 2. Nebuliser retainer, 3. Air tubing, 4. Constant head & drain, 5. Fuel tubing.
6. End Cap, 7. Securing Screw.
4. Connect the length of rubberised fuel tubing (001 72 114) between the inlet
connector on the internal regulator, (item 5, figure 2.2) and the regulator at the
cylinder outlet connector. Secure with 'Unex' tubing clamps (001 08 718).
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2
Installation
Continued
Figure 2.2 Rear Connections for Services
1. Regulator outlet connector, 2. Air inlet, 3. Air regulator adjuster,
4. Gas inlet connector, 5. Gas Regulator inlet connector.
5. Turn on the fuel supply and check all connectors for leaks, using soap solution.
Do not use the instrument until you are satisfied that the installation is leak
proof.
6. Connect the air compressor to a suitable mains supply.
7 Connect the length of reinforced hose (001 72 043) between the air compressor
outlet and the air inlet connector on the rear panel (item 2, figure 2.2). Secure
both ends with 'Unex' tubing clamps (001 08 718).
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Installation
continued
Figure 2.4 Filling the’U’ tube
Wash bottle, 2. Constant head & drain, 3. ‘U’ tube, 4. Drain tube, 5. Spring Clip
8 Fit one of the 200mm lengths of drain tubing (item 4, figure 2.4) to the outlet
on the constant head and drain. If necessary connect a suitable length of tubing
(not supplied), to extend the drain tubing to carry waste to a sink or other drain
receptacle. The downward flow of waste must not be restricted. The second
length of tubing supplied is a spare for either the 'U' tube or drain tube.
9 Make sure that the constant head and drain is positioned so clip that the lip at
the top is resting on the spring clip. Use a wash bottle (item 1, figure 2.4) to fill
the 'U' tube with deionised water. Sufficient water should be used to purge the
tube of air. Allow excess water to flow back into the drain.
IMPORTANT Do not continue until you are satisfied that the 'U' tube has been
completely filled with water, and is purged of air.
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10. Plug in the external Power supply to the inlet shown on Fig 2.3.
Installation
continued
11. Fit a suitable a.c. supply lead to the External Power supply.
CAUTION Ensure that the green/yellow earth connector in the a.c. supply lead
is connected to a properly grounded earth point, the brown lead is connected to the
Line terminal (L) and blue lead is connected to the Neutral terminal (N).
12. If necessary, set the “1/0" power switch (below the chimney) to the 0 position.
and connect the plug to a convenient supply socket.
13. If no printer/computer or chart recorder interfacing is required proceed to
Chapter 6 of this manual.
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Installation
continued
2.5 Connecting Peripheral Devices
Printer
Cole-Parmer can supply a serial printer set up to operate with the Model 02655-10 &
Model 02655-15 (Part # 473 56 903. This should be connected to the cable supplied
with the instrument, the other end of which is connected to the rear panel in the RS232
socket as shown in Fig 2.3.
Computer
The same serial outlet and cable can be connected to a computer, which, through the
“terminal” function, will display a .txt file identical to the printer display.
Sherwood Scientific now supply an Excel spreadsheet program which can take the
output from the Model 02655-10 or Model 02655-15 directly into a spreadsheet for
further manipulation
Analogue Devices
The Model 02655-10/15 have one set of analogue outputs corresponding to each
channel.
The left hand coaxial socket takes the output from Channel 1 and the right hand socket
from Channel 2. The appropriate voltages are set as described in Section 2.10.
Auto-sampler
The Model 02655-10/15 can be augmented with an Auto-sampling device to form a
fully automatic analytical system. The Model 860 Autosampler has 40 positions for
serum and other clinical samples and is used in conjunction with the Model 805
Automatic Diluter.
The Autosampler is supplied with a signal cable which is plugged in to the Rear panel
as shown in Fig 2.3.
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