Cole-Parmer 02655-10, 02655-15 Operator Manual

Download

Model 02655-10

And

Model 02655-15

Flame Photometer

Operator Manual

Cole-Parmer Instrument Company

625 East Bunker Court

Vernon Hills

IL 60061

USA

425 91 002 Issue 9, 6th August 2008 ECN 527

G:\DOCUMENT CONTROL\Manuals\Flame\425\42591002 Cole Parmer M420 & M425 Operator Manual Issue 9.doc

Model 02655-10 & 02655-15 Flame Photometer

Short Form Instructions Model 02655-10/15 Flame Photometer

The Default mode for the Model 02655-10/15 that will appear when the instrument is switched on is: Dual channel Sodium and Potassium with Peak detection and Reference mode selected. These instructions are to be used in the default mode.

NB In Reference Mode the Blank solution as well as the Calibration Standard

and Sample must contain 100ppm Li (Use the Blank solution to dilute the Standards and Samples).

Operation: -

Set Up and Ignition

1. Ensure that the ‘U’ tube is full of deionised water.

2. Connect the gas supply (maximum 30psi Propane or Butane) via a Primary regulator.

3. Switch on the compressor and regulate the air pressure to 11psi on the gauge at the rear of the instrument.

4. Switch on the instrument with the “1/O” switch.

5. If the flame ignites then an auto sequence will test various circuits and end with the ‘000’ flashing in both channel displays.

6. If the flame does not ignite, switch off, wait 15 - 20 seconds and repeat step 4. It may take 3 or 4 attempts if the instrument has not been used for some time.

Blanking (Zeroing)

7. With the ‘000’ flashing aspirate the blank solution and press the ‘Blank’ button once.

8. The instrument will then zero to the blank values; this will take at least 30 seconds; Remove the blank when complete.

Calibration

9. When the blanking procedure is complete, the previous calibration values that were used will appear in the displays and the ‘Cal’ indicators will flash. If you wish to calibrate to these values go to 11.

10. If you wish to change the values use the ‘+ and –’ keys before aspirating the standard solution.

11. Then press the Sodium ‘Set’ button, then start aspirating the standard and press the Potassium ‘Set’ button within a few seconds.

12. Once the instrument has calibrated itself then the ‘Cal’ lights will flash; remove the standard and press ‘Set’ on both channels again.

Measuring a Sample

13. After 5 seconds both displays will flash and the “Measure” light will illuminate, the first sample can now be measured.

14. Aspirate the sample and then immediately press the ‘Print/Measure’ button.

15. The instrument will measure the sample until the peak algorithm captures the value and the measure light shows continuously. Remove the sample.

16. After 5 seconds the displays will flash and the next measurement can be made.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 2 -

Model 02655-10 & 02655-15 Flame Photometer

Table of Contents

Page

Short Form Instructions Model 02655-10/15 Flame Photometer .................................................. 2

Operation: - ................................................................................................................................. 2

1 INTRODUCTION ........................................................................................................... 6

1.1 Contacting Cole-Parmer ...................................................................................... 6

1.2 Using this manual ............................................................................................... 6

1.3 Safety Precautions ............................................................................................... 7

1.4 Reagents ............................................................................................................. 8

Reference/Lithium Standard ................................................................................ 8

Blank Storage...................................................................................................... 8

Purification ......................................................................................................... 8

1.5 Dilutions ............................................................................................................. 8

Dilution Ratio ..................................................................................................... 9

Urine Samples ..................................................................................................... 9

2 INSTALLATION .......................................................................................................... 10

2.1 Location ............................................................................................................ 10

2.2 Services Required .............................................................................................. 10

Electrical Supply ............................................................................................... 10

Fuel ................................................................................................................... 10

Air .................................................................................................................... 10

Waste Container ................................................................................................ 10

2.3 Unpacking......................................................................................................... 11

Accessory List ................................................................................................... 11

2.4 Assembly .......................................................................................................... 12

2.5 Connecting Peripheral Devices .......................................................................... 16

Printer ............................................................................................................... 16

Computer .......................................................................................................... 16

Analogue Devices.............................................................................................. 16

Auto-sampler .................................................................................................... 16

2.6 Setting Date and Time....................................................................................... 17

2.7 Setting Filter Factors ......................................................................................... 18

2.8 Setting Units ..................................................................................................... 18

2.9 Setting Sample Delay (in Peak mode) ................................................................ 19

2.10 Setting the Output Voltage ................................................................................ 19

2.11 Setting the analogue damping coefficient. ......................................................... 19

3 PRINCIPLES OF OPERATION ..................................................................................... 20

3.1 Application of Flame Photometry ...................................................................... 20

3.2 Principles of Flame Photometery ....................................................................... 20

3.3 The Internal Standard Mode .............................................................................. 21

4 PERFORMANCE CHARACTERISTICS & SPECIFICATIONS ................................... 22

4.1 Readout ............................................................................................................. 22

4.2 Measurement Ranges ........................................................................................ 22

4.3 Specificity ......................................................................................................... 23

4.4 Accuracy ........................................................................................................... 23

Linearity ........................................................................................................... 23

Drift .................................................................................................................. 23

Reproducibility (using Peak mode) .................................................................... 23

Using Peak with Ref (Internal Standard) Mode.................................................. 23

4.5 Warm Up .......................................................................................................... 24

4.6 Sample Requirements ........................................................................................ 24

Type .................................................................................................................. 24

Method of Presentation...................................................................................... 24

Volume ............................................................................................................. 24

4.7 Chart Recorder Output ...................................................................................... 25

425 91 002 Issue 9, 6th August 2008 ECN 527

- 3 -

Model 02655-10 & 02655-15 Flame Photometer

Page

4.8 Environmental Conditions................................................................................. 25

Temperature ...................................................................................................... 25

Humidity ........................................................................................................... 25

4.9 Power Requirements .......................................................................................... 25

Voltage ............................................................................................................. 25

Power ................................................................................................................ 25

4.10 Fuel ................................................................................................................... 25

4.11 Air .................................................................................................................... 26

4.12 Size ................................................................................................................... 26

4.13 Weight .............................................................................................................. 26

5 DESCRIPTION OF INSTRUMENT .............................................................................. 27

5.1 Front Panel Controls and Indicators .................................................................. 27

Primary Function .............................................................................................. 27

Secondary Function ........................................................................................... 27

Tertiary Function .............................................................................................. 27

Quaternary Function ......................................................................................... 27

Blank ................................................................................................................ 27

Channel selector ................................................................................................ 27

Mode Selector ................................................................................................... 27

Measure/Print.................................................................................................... 27

Channel 1 Set .................................................................................................... 27

Channel 2 Set .................................................................................................... 27

5.1.1 Controls ............................................................................................................ 27

‘-’ ‘Set’ ‘+’ ..................................................................................................... 27

(Feedback beep confirms the action) .................................................................. 27

Blank ................................................................................................................ 28

Single/Dual and Element Selection ................................................................... 28

Peak/Ref/Cont ................................................................................................... 28

Measure ............................................................................................................ 29

5.1.2 Description of Indicators ................................................................................... 29

Cal (Ch1 & Ch2) ............................................................................................... 29

Single................................................................................................................ 29

Dual .................................................................................................................. 29

Peak .................................................................................................................. 29

Ref .................................................................................................................... 29

Cont .................................................................................................................. 30

Measure ............................................................................................................ 30

Flame On .......................................................................................................... 30

Power On .......................................................................................................... 30

5.2 Rear Panel Controls and Connections ................................................................ 30

Channel 1 & 2 Data Outputs ............................................................................. 31

RS232/Printer Output ........................................................................................ 31

External Device Output ..................................................................................... 31

Power ................................................................................................................ 31

Gas ................................................................................................................... 31

Air .................................................................................................................... 31

Air Regulator .................................................................................................... 31

5.3 Operating Modes ............................................................................................... 32

5.3.1 Single/Dual Mode ............................................................................................. 32

Single................................................................................................................ 32

Dual .................................................................................................................. 32

Blanking ........................................................................................................... 32

Calibration ........................................................................................................ 32

5.3.2 Peak/Cont ......................................................................................................... 33

Peak .................................................................................................................. 33

The Peak Cycle ................................................................................................. 33

425 91 002 Issue 9, 6th August 2008 ECN 527

- 4 -

Model 02655-10 & 02655-15 Flame Photometer

5.3.3 Ref Mode .......................................................................................................... 34

Cont .................................................................................................................. 34

5.3.4 Blank ................................................................................................................ 34

6 OPERATING THE INSTRUMENT .............................................................................. 35

6.1 Start Up ............................................................................................................ 35

6.1.1 Compressor Start Up ......................................................................................... 35

6.1.2 Switch On ......................................................................................................... 35

6.1.3 Ignition Successful ............................................................................................ 35

6.2.1 Required Blanking ............................................................................................ 35

6.2.2 Required Calibration ......................................................................................... 36

6.3 Sample Measuring ............................................................................................ 37

6.3.1 Measuring in Peak mode ................................................................................... 37

6.3.2 Measuring in Continuous mode ......................................................................... 37

6.4 Automatic operation .......................................................................................... 37

6.5 Sample Numbering ........................................................................................... 38

6.5.1 Reset of sample number..................................................................................... 38

6.5.2 Repeat sample ................................................................................................... 38

6.5.3 QC sample ........................................................................................................ 38

6.6 Shutdown Procedure.......................................................................................... 38

7 OPERATING HINTS, PRECAUTIONS & LIMITATIONS ........................................... 39

7.1 Hints ................................................................................................................. 39

7.2 Operational Precautions and Limitations ........................................................... 39

7.3 Hazards ............................................................................................................. 40

7.4 Measuring Calcium ........................................................................................... 40

8 CLEANING AND MAINTENANCE PROCEDURES ................................................... 41

8.1 General ............................................................................................................. 41

8.2 Daily Maintenance ............................................................................................ 41

8.3 Weekly Maintenance ......................................................................................... 41

8.4 Monthly Maintenance ....................................................................................... 41

8.5 Six Monthly Maintenance ................................................................................. 42

8.6 Nebuliser ........................................................................................................... 42

8.6.1 Operational Check ............................................................................................ 42

8.6.2 Cleaning the Nebuliser ...................................................................................... 43

8.7 Cleaning the Mixing Chamber, Burner and Drain ............................................. 43

8.7 Cleaning the Mixing Chamber, Burner and Drain continued ............................. 44

8.8 Cleaning the Glass Chimney ............................................................................. 44

8.10 Deproteinising or Disinfecting Procedure .......................................................... 45

9 ACCESSORIES, CONSUMABLES & SPARE PARTS ................................................. 46

9.1 Ordering Information ........................................................................................ 46

9.2 Spares and Accessories ...................................................................................... 46

9.3 Reagents ........................................................................................................... 47

APPENDIX A ........................................................................................................................... 48

BIBLIOGRAPHY ...................................................................................................................... 48

APPENDIX B ........................................................................................................................... 49

APPENDIX C ........................................................................................................................... 51

Cole Parmer Instruments Warranty Statement ........................................................................... 52

425 91 002 Issue 9, 6th August 2008 ECN 527

- 5 -

Model 02655-10 & 02655-15 Flame Photometer

1 Introduction

1.1 Contacting Cole-Parmer

Postal address for letters and orders:

Cole-Parmer Instrument Company 625 East Bunker Court Vernon Hills IL 60061 USA Telephone 847 549 7600 Toll Free 800 323 4340 Fax 847 247 2929

For Certificates of Analysis and Material Safety Data Sheet downloads, contacting Distributors and Technical and Application information on our products go to our website

www.coleparmer.com

The following section must be carefully read before unpacking, installing, using, maintaining or repairing the instrument and/or its accessories.

1.2 Using this manual

This manual is structured to allow both quick access to a measurement from the default position of the Instrument (refer to Section entitled Short Form Instructions on page 2) and an in depth understanding of the Set-up, Operation and Maintenance of these two advanced Flame Photometers.

The information contained in this manual was correct at the time of going to print. However, Cole-Parmers’ policy is one of continuous product improvement and the right to change specifications, equipment and maintenance procedures at any time, without notice, are reserved. This manual is copyrighted, and all rights are reserved. No part of this manual may be reproduced by any means or in any form without prior consent in writing.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 6 -

Model 02655-10 & 02655-15 Flame Photometer

Introduction

continued

1.3 Safety Precautions

The Model 02655-10 and 02655-15 are intended for use by persons knowledgeable in safe laboratory practices. If the instruments are not used in accordance with these “Instructions for use”, the protection provided by the equipment may be impaired.

WARNING The Model 02655-10/15 are designed to be earthed (grounded) through the power supply lead (line cord) for safe operation. For the safety of operating personnel and optimum performance make sure that the instrument is only connected to a 3-pin socket (outlet) that has an effective earth connection. If you are in any doubt about the safety of your electrical supply system consult a competent, qualified electrician.

There are no user replaceable parts within the instrument. Do not remove the rear cover from the instrument.

Cole-Parmer Instrument Company and its authorised Distributors consider themselves responsible for the effects of safety, reliability and performance of the Model 02655-10 and 02655-15 only if: ** Assembly operations, extensions, re-adjustments, modifications or repairs are only carried out by

persons authorised by them.

** The electrical installation of the relevant room complies with IEC requirements or the local regulatory

code.

** The equipment is used in accordance with the instructions for use.

PLEASE NOTE that no cables neither for power-in nor for signals-out to the printer, computer via RS232 output nor analogue to a chart recorder, nor signal cables from an Autosampler nor any other device should exceed 2.5 metres in length.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 7 -

Model 02655-10 & 02655-15 Flame Photometer

Introduction

continued

1.4 Reagents

Cole-Parmer supply a wide range of reagents, including standards, diluent and maintenance solutions, for use with the Model 02655-10 and 02655-15 Flame Photometers. Please refer to Section 9.3 for a complete list of the reagents available.

Reference/Lithium Standard

The full benefit of the technology incorporated in the Model 0655-10/15 is utilised in the default mode of operation. This mode, ‘Peak’ and ‘Reference’, requires that the blank, standards and samples contain Lithium at the concentration of approximately 100ppm in the final solution that is aspirated. This is best achieved if the diluent contains 100ppm Lithium. That is 100ml of the 1000ppm Li standard solution supplied with the instrument (Part No. 001 56 622) diluted to 1 litre with the diluent described in the following paragraph.

Blank Storage

All solutions should be stored away from direct sunlight, in a cool place (below 25°C/77°F), in an airtight container to prevent evaporation and discolouration. Glass containers should not be used, as they can affect Na concentration levels. Prolonged exposure to the atmosphere must be avoided to prevent evaporation of standard solutions.

Purification

No purification is required for Cole-Parmer standard solutions.

1.5 Dilutions

Samples and standards should be diluted with the same batch of diluent, made up of 1 part Diluent Concentrate (Part # 001 56 181) to 999 parts good quality distilled water. The same batch of diluent should be used to zero the Model 02655-10 and Model 02655-15 and to prepare dilutions of standards and samples. This will prevent variations in water purity affecting the measurements. Great care should be taken so that contamination does not occur when preparing the samples and standards. Remember that the accuracy of the instrument is dependent on the accuracy and purity of the Standards and Blank used for calibration.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 8 -

Model 02655-10 & 02655-15 Flame Photometer

Introduction

continued

Dilution Ratio

The Model 02655-10/15 are optimised for the following Dilution Ratios: -

In Clinical Mode Na & K both Serum and Urine: - 1:200 with diluent described above

(including Li if used in Reference Mode).

Li (Measurement) Dilution Ratio: - 1:50.

In Non Clinical Mode

The user should ascertain from his sample what is appropriate by reference to

the input concentrations listed in the specification in Section 4 pp 19/20.

To aid the dilution of clinical samples Cole-Parmer produce a Dilutor, the Model 805, which automates the process and gives extremely reproducible dilution ratios and therefore precise results.

Urine Samples

The Potassium content of urine samples is some 20 times that of plasma or serum. In the past it has been necessary to pre-dilute urine samples prior to following a normal dilution procedure with e.g. Model 805. The design of the Model 02655-10/15 however, minimises the need for this pre-dilution and urine samples with a Potassium concentration of 120mmol/l or less can be treated in exactly the same way as plasma and serum samples provided that the appropriate standard is used to calibrate the instrument for the high Potassium value. Samples with a Potassium level greater than 120mmol/l should be pre-diluted 1 part in 5 prior to the normal dilution 1 part in 200.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 9 -

Model 02655-10 & 02655-15 Flame Photometer

2 Installation

2.1 Location

WARNING Under no circumstances install the Model 02655-10/15 beneath

overhanging cupboards. There must be at least 1 metre of clear space above the chimney. For optimum performance, this instrument should be installed in accordance with the following conditions: -

2.1.1 The environment must be clean and free from dust.

2.1.2 The instrument must be placed on a strong, level worktop, free from vibration. The Model 02655-10/15 requires approximately 500mm x 500mm of bench space, which includes an area in front for solutions and clearance at the rear for fuel and air tubing, with clear access to the mains supply switch.

2.1.3. Avoid sites that expose the instrument to direct sunlight or draughts.

2.1.4. To meet the specification the ambient temperature must be within the

range +10°C to +35°C and the maximum relative humidity must not be more than 85%, non-condensing.

2.2 Services Required

Electrical Supply

An A.C. supply between 90V and 240V ±10%, at 50Hz or 60Hz, is required for the Model 02655-10 and 02655-15. The instrument is powered from an external power supply, which will accommodate these voltages without any need to adjust the instrument.

Fuel

A supply of Propane, Butane or Propane/Butane mixture regulated at the cylinder to

2.1kg/cm2 (30psi), flow rate at least 0.4 litres per minute. (NB. Propane only, gives the best results for Ca on the Model 02655-15). Primary regulators to fit cylinders are available from Cole-Parmer, regulators are available as optional accessories, refer to Section 9.2.

Air

A supply of clean, dry, oil-free air at 1kg/cm2 (11psi), flow rate 6 litres per minute. A suitable air compressor is listed in Section 9.2.

Waste Container

A sink or waste container sited to the right of the instrument will ensure the minimum length of waste tubing. Do not use a waste container with high sides, as this will cause the drain tube to be lifted above the level of the constant head drain.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 10 -

Model 02655-10 & 02655-15 Flame Photometer

Installation

continued

2.3 Unpacking

1. Unpack the instrument and accessories. NOTE The Model 02655-10/15 weighs 9.5kg; follow safe lifting techniques.

2. Check all items for damage.

3. Check that all the items on the Accessory List have been delivered. Contact your Sherwood Scientific distributor if you have any problems.

4. The Model 02655-10/15 is shipped with the following items: -

Accessory List

CAT. NO. DESCRIPTION QUANTITY 001 72 043 Air tubing, nylon reinforced 2 metres

001 72 114 Fuel tubing 2 metres 400 22 002 Drain tube, 200 mm, and spare tube 200mm 1 pack 001 53 420 Universal external power supply 1 100 99 010 Nebuliser cleaning wire, pack of 3 1 pack 001 31 076 'O' ring, spare for mixing chamber 1 420 08 102 Nebuliser 1 400 22 003 Nebuliser inlet tube, polythene, 150 mm 1 425 91 002 Operators manual 926 09 052 RS232 Output Cable 1 001 08 718 'Unex' tubing clamp 4 001 56 100 Standard Solution containing l40.0mmol/l Na,

5.00mmol/l K and 1.5mmol/l Li 001 56 622 Lithium 1000ppm Standard, 6 x 100ml 1 pack 001 56 620 Sodium 1000ppm Standard, 6 x 100ml 1 pack ** 001 56 621 Potassium 1000ppm Standard, 6 x 100ml 1 pack ** 001 56 623 Calcium 1000ppm Standard , 6 x 100 ml 1 pack *** 410 92 001 CDROM, Flame Training Guide 1

* supplied with Clinical instruments. ** supplied with Industrial instruments. *** supplied with Model 02655-15.

1 x 100 ml*

425 91 002 Issue 9, 6th August 2008 ECN 527

- 11 -

Model 02655-10 & 02655-15 Flame Photometer

Installation

continued

2.4 Assembly

EQUIPMENT REQUIRED: 'Pozidriv' (cross head) screwdriver 1PT Flat blade screwdriver.

To assemble the instrument, proceed as follows: -

1. Lift off the chimney cap from the chimney assembly and lift out the glass

chimney. Remove any packing material from inside, making sure that there are no small pieces left within the chimney.

2. Replace the glass chimney with the clear strip at the bottom and refit the

chimney cap on top of the chimney.

3. Remove the Nebuliser (420 08 102) from its box and push the barbed

connector in to the end of the air tubing (item 3, figure 2.1). Fit the Nebuliser (item 1, figure 2.1) to the mixing chamber and position the retainer (item 2, figure 2.1) to lock it into position. Locate the Nebuliser inlet tubing (400 22

003) and fit over the narrow tube in the front of the Nebuliser.

Figure 2.1 Mixing Chamber

1. Nebuliser, 2. Nebuliser retainer, 3. Air tubing, 4. Constant head & drain, 5. Fuel tubing.

6. End Cap, 7. Securing Screw.

4. Connect the length of rubberised fuel tubing (001 72 114) between the inlet

connector on the internal regulator, (item 5, figure 2.2) and the regulator at the cylinder outlet connector. Secure with 'Unex' tubing clamps (001 08 718).

425 91 002 Issue 9, 6th August 2008 ECN 527

- 12 -

Model 02655-10 & 02655-15 Flame Photometer

Installation

Continued

Figure 2.2 Rear Connections for Services

1. Regulator outlet connector, 2. Air inlet, 3. Air regulator adjuster,

4. Gas inlet connector, 5. Gas Regulator inlet connector.

5. Turn on the fuel supply and check all connectors for leaks, using soap solution.

Do not use the instrument until you are satisfied that the installation is leak proof.

6. Connect the air compressor to a suitable mains supply. 7 Connect the length of reinforced hose (001 72 043) between the air compressor

outlet and the air inlet connector on the rear panel (item 2, figure 2.2). Secure both ends with 'Unex' tubing clamps (001 08 718).

425 91 002 Issue 9, 6th August 2008 ECN 527

- 13 -

Model 02655-10 & 02655-15 Flame Photometer

Installation

continued

Figure 2.4 Filling the’U’ tube

Wash bottle, 2. Constant head & drain, 3. ‘U’ tube, 4. Drain tube, 5. Spring Clip

8 Fit one of the 200mm lengths of drain tubing (item 4, figure 2.4) to the outlet

on the constant head and drain. If necessary connect a suitable length of tubing (not supplied), to extend the drain tubing to carry waste to a sink or other drain receptacle. The downward flow of waste must not be restricted. The second length of tubing supplied is a spare for either the 'U' tube or drain tube.

9 Make sure that the constant head and drain is positioned so clip that the lip at

the top is resting on the spring clip. Use a wash bottle (item 1, figure 2.4) to fill the 'U' tube with deionised water. Sufficient water should be used to purge the tube of air. Allow excess water to flow back into the drain.

IMPORTANT Do not continue until you are satisfied that the 'U' tube has been

completely filled with water, and is purged of air.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 14 -

Model 02655-10 & 02655-15 Flame Photometer

10. Plug in the external Power supply to the inlet shown on Fig 2.3.

Installation

continued

11. Fit a suitable a.c. supply lead to the External Power supply.

CAUTION Ensure that the green/yellow earth connector in the a.c. supply lead is connected to a properly grounded earth point, the brown lead is connected to the Line terminal (L) and blue lead is connected to the Neutral terminal (N).

12. If necessary, set the “1/0" power switch (below the chimney) to the 0 position.

and connect the plug to a convenient supply socket.

13. If no printer/computer or chart recorder interfacing is required proceed to

Chapter 6 of this manual.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 15 -

Model 02655-10 & 02655-15 Flame Photometer

Installation

continued

2.5 Connecting Peripheral Devices

Printer

Cole-Parmer can supply a serial printer set up to operate with the Model 02655-10 & Model 02655-15 (Part # 473 56 903. This should be connected to the cable supplied with the instrument, the other end of which is connected to the rear panel in the RS232 socket as shown in Fig 2.3.

Computer

The same serial outlet and cable can be connected to a computer, which, through the “terminal” function, will display a .txt file identical to the printer display. Sherwood Scientific now supply an Excel spreadsheet program which can take the output from the Model 02655-10 or Model 02655-15 directly into a spreadsheet for further manipulation

Analogue Devices

The Model 02655-10/15 have one set of analogue outputs corresponding to each channel. The left hand coaxial socket takes the output from Channel 1 and the right hand socket from Channel 2. The appropriate voltages are set as described in Section 2.10.

Auto-sampler

The Model 02655-10/15 can be augmented with an Auto-sampling device to form a fully automatic analytical system. The Model 860 Autosampler has 40 positions for serum and other clinical samples and is used in conjunction with the Model 805 Automatic Diluter.

The Autosampler is supplied with a signal cable which is plugged in to the Rear panel as shown in Fig 2.3.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 16 -

Model 02655-10 & 02655-15 Flame Photometer

Installation

continued

2.6 Setting Date and Time

Press and hold ‘Print’ during the start up sequence to put the instrument into Date and Time Mode, which will be operative as soon as the flame has lit. Ch1 displays the date format "day month year". Display day as ‘d 10’.

Adjust with ‘- or +’.

Press 'Set' to confirm and move on.

Display month as ‘n 05’.

Adjust with ‘- or +'.

Press 'Set' to confirm and move on.

Display year as ‘y 98’.

Adjust with ‘- or +’.

Press 'Set' to confirm and move on.

Display hour as ‘h 13’.

Adjust with ‘- or +'.

Press 'Set' to confirm and move on.

Display minutes as ‘n 40’.

Adjust with ‘- or +’.

Press 'Set' to confirm and move on.

Automatically sends output as in format: day = 10 month = 05 year = 98 hours = 13 minutes = 40

The instrument now reverts back to the point in the operating sequence waiting for the first blank.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 17 -

Model 02655-10 & 02655-15 Flame Photometer

Installation

continued

2.7 Setting Filter Factors

The Model 02655-10 uses three detectors permanently connected; one each for Sodium, Potassium and Lithium. (NB The Model 02655-15 has four detectors with the extra Calcium channel). Each detector has an interference filter of the appropriate wavelength fitted in front it. The filters have a unique transmittance factor, which is measured during construction and entered into the software. These values are held in a battery-backed memory. If the battery fails, shown by Error Code ‘E62’, it is necessary to re-introduce the factors by the following procedure. The correct Filter Factors can be obtained from Cole-Parmer by reference to the Serial number.

1. Press then hold down the ‘Set’ key, the ‘Blank’ key and the ‘Print/Measure’

key together and wait for the ‘beep’. After a few seconds the top display will show 1000 and the lower display will show F1.

2. Adjust the top display using the ‘+ and –’ controls to the value for the Sodium

(Na) filter. Press ‘Set’ in the top display to fix the value. This will then reveal 1000 in the top display and F2 in the lower display.

3. Repeat step 2 for F2 Potassium (K) value. 4 Repeat step 2 for F3 Lithium (Li) value.

5. Repeat step 2 for F4 Calcium (Ca) value

2.8 Setting Units

The default mode for the Model 02655-10 and Model 02655-15 is to print results as well as blank and calibration values in mmol/l units. It is possible to change this option so that no units are printed.

1. After switch on or at any time press and hold down the ‘Blank’ key until ‘101’

is displayed in Channel 1.

2. To remove the units change the display to 100 using the “-” button.

3. Press ‘Set’ to accept the new value.

4. The display will now show 200 in Channel 1, which allows sample delay see

Section 2.9 (below).

425 91 002 Issue 9, 6th August 2008 ECN 527

- 18 -

Model 02655-10 & 02655-15 Flame Photometer

Installation

continued

2.9 Setting Sample Delay (in Peak mode)

In Peak mode, the instruments have a default delay of 19 seconds after pressing ‘Cal’ or ‘Print’ before the Peak-seeking algorithm initiates; this allows the calibrant/sample to stabilise in the flame. This time delay can be adjusted by the user to accommodate, for example, a diluter where there is a considerable delay before such stabilisation can take place. (The length of this delay can be ascertained by running the instrument in continuous mode and observing the exact time between introducing the sample and the stabilisation of the display).

1. Following the setting or acceptance of the units as described in Section 2.8

(above) the display reads ‘200’ in Channel 1.

2. Press ‘+’ in Channel 1 to display the delay value in Channel 2.

3. If the default delay of 19 seconds is acceptable press ‘Set’.

4. If not, adjust the value in Channel 2 by pressing the ‘+’ key until the value is

correct and then press ‘Set’ on Channel 1.

5. The display will now show ‘300’ in Channel 1. Which allows the output voltage

to be adjusted, see Section 2.10 (below).

NB It is essential while operating in Peak Mode to aspirate the standard or sample before pressing “Cal” or “Print” to take advantage of this facility.

2.10 Setting the Output Voltage

The output voltage is the maximum voltage measured when at full scale. This can be set from 0.05 to 7.5.

1. Following the setting or acceptance of the sample delay as described in Section 2.9,

Channel 1 display reads ‘300’, and Channel 2 ‘1.0’ (default).

2. Adjust the output voltage in Channel 2 using the ‘+ or -’ buttons.

3. Accept the required output voltage by pressing ‘Set’ in Channel 1. (NB The Model 02655-15 has an additional feature, which allows the analogue outputs

to be set for blank and “span” by passing the normal calibration protocol. This feature can be invoked by adjusting the 300 to 301 by the ‘+’ key before pressing “SET” as above).

Setting the analogue damping coefficient.

1 After adjusting the voltage level in section 2.10 above, the instrument shows

“400” in the Channel 1 display.

2 To adjust the level of damping in the analogue output adjust the channel 2 display

from 1 (No damping) to 20 (Maximum Damping) by use of the ‘+ & –‘ keys. Press ”SET”.

The displays will return to flashing ‘000’ and the blanking and calibration procedures can begin again.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 19 -

Model 02655-10 & 02655-15 Flame Photometer

3 Principles of Operation

3.1 Application of Flame Photometry

Prior to the advent of flame photometry, Sodium and Potassium were typically determined gravimetrically after the precipitation of relatively insoluble salts such as Sodium Uranyl Zinc Acetate (Ref. 1) and Potassium Chloroplatinate (Ref. 2). As with all chemical methods for these two elements there were cross interferences and also interference from other ions such as NH4+. Many analytical steps such as protein precipitation or ashing of the sample were involved with all the attendant losses and inaccuracies and the complete procedures required many hours.

In many applications, particularly in clinical situations, rapid availability of results is of prime importance. By flame photometry both Sodium and Potassium results on a single sample can be available in less than 5 minutes of the sample reaching the laboratory. With the availability of the Li Reference Mode accurate results can be achieved while the instrument is still warming up. A simple dilution step is all that is required; therefore sample handling, losses and inaccuracies are at a minimum (Ref. 3 to 10).

3.2 Principles of Flame Photometery

When a solution is aspirated into a low temperature flame, in an aerosol, each droplet of water evaporates leaving a solid core of the residue of evaporation. The core further breaks down to the molecular level, and provided the molecules are not too refractory, progress to form atomic species. The atom then is excited by the flame and its electron temporarily moves to a higher energy state.

When the electrons return to the ground state, they lose the excitation energy and a discrete wavelength of visible light is emitted, characteristic of the atom. The emitted light can be isolated from other light wavelengths by an optical filter. The amount of light being emitted is proportional to the number of atoms in the flame, and it follows, the concentration of that atom in the original solution. The amount of light emitted can be measured by a suitable photodetector.

The photodetector generates an electrical signal which is amplified and displayed on a digital readout.

The Cole-Parmer Model 02655-10 Flame Photometer is a Dual Channel low temperature Flame Photometer designed to be used for the simultaneous determination of Sodium (Na), and Potassium (K) in Clinical and Industrial samples. The Model 02655-10 also has the benefit of an Internal Standard using a Lithium (Li) signal to eliminate interferences due to variation in dilution ratios (where a continuous Diluter is used) as well as variability in the characteristics of the flame photometer itself. The Model 02655-10 can also function as a Single Channel instrument for the determination of Na and K including reference mode and Li without reference mode. The Model 02655-15 is a Four element Flame Photometer where the user can choose to display any two of the following elements Na, K, Li and Ca. (or two from Na, K, Ca with Li Internal Standard.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 20 -

Model 02655-10 & 02655-15 Flame Photometer

Principles of Operation

continued

3.3 The Internal Standard Mode

When the Internal Standard is invoked with the ‘Ref’ mode, this uses the signal from the Li detector to scale the readings from the other detector(s) to allow for external effects such as drift, flame disturbances, changes in dilution, nebuliser uptake rate, etc. It works by adding a known concentration, (Co), of Li to the Blank, and the diluent of the Standard solutions and the Sample. The Li signal is constant at Co if all the external effects are constant. If the Li signal changes it does so because of flame and instrument variables. By introducing a factor based on the reference Li measurement, we can remove many of these variables from the analysis of Sodium and Potassium. The Li reference factor is assigned a value of 1.000 at the time of calibration. This factor is shown on the print-out each time an analysis is performed, which indicates the amount of change due to drift, aspiration rate etc., since the calibration was made. The limit of ±20% can be corrected for and if the factor exceeds this limit an error is indicated which tells the operator to clean the aspirator and recalibrate. To use this mode the blank, calibration standard, and samples will have to be in a solution that contains Lithium of a constant concentration, Co, in the diluent. See Section 1.3.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 21 -

Model 02655-10 & 02655-15 Flame Photometer

4 Performance Characteristics &

Specifications

The Model 02655-10 can be ordered in two versions: the Model 02655-10 Clinical and the Model 02655-10 Industrial. The following specification applies to the Clinical version. The Model 02655-15 is offered in the non-clinical mode although clinical standards can be supplied separately see Section 9.

The specification of the Industrial version is included as an appendix to this manual.

4.1 Readout

Dual three-digit light emitting diode (LED) display, 12.5 mm high. Display ranges 0 to 199.9.

NOTE If negative values are displayed the instrument is operating outside of the recommended measurement range or it is in between samples in continuous reference Mode as the Lithium reference is absent.

4.2 Measurement Ranges

Model Model 02655-10 Model 02655-15 Elements analysed Na, K (with Li Int Std)

Sample concentration Na 0.5 to 40ppm

K 0.5 to 20ppm Li 0.0 to 0.6ppm

Measurement ranges ppm All 1.00-

9.99,10.0-199.9 mmol/l as above except

Li only 1.00-5.00 Readout No Units or mmol/l No Units or mmol/l Sensitivity for 100 displayed

Reproducibility <± 1.0% <± 1.0% Reading Stabilisation Variable software

Recorder output 2 outputs 50mV-7.5V

Air Supply (required) 6 liters/min @ 11 psi oil

Na and K= 0.5ppm,

Li = 2 ppm

command

via software command

and moisture free

Na, K& Ca (with Li Int Std) Li Na 0.5 to 40ppm K 0.5 to 20ppm Li 0.0 to 0.6ppm Ca 5 to 100ppm ppm All 1.00-

9.99,10.0-199.9 mmol/l as above except Li only 1.00-5.00

Na and K= 0.5ppm, Li = 2 ppm Ca = 5ppm

Variable software command 2 outputs 50mV-7.5V via software command 6 liters/min @ 11 psi oil and moisture free

425 91 002 Issue 9, 6th August 2008 ECN 527

- 22 -

Model 02655-10 & 02655-15 Flame Photometer

Performance Characteristics and Specification

continued

4.3 Specificity

For Na, K and Li measurements, interference will be less than 0.5% from a concentration of Na, K, and Li, equal to the concentration of the element under test.

For calcium there is a significant background interference from Sodium : The emission of a 100ppm Calcium solution will be increased by approximately 20% in the presence of a 100ppm Sodium solution. This will be reduced to 13% if a Lithium internal standard reference is used. The effect is linear so a correction factor can be used if a mixture of Calcium and sodium is to be analysed.

4.4 Accuracy

Linearity

Better than 1% measured at mid-range, when standardized at the following concentrations: - 140.0mmol/, Na; 5.0mmol/l K; 1.5mmol/l Li.

Drift

NOTE A minimum 15 minutes warm up must be allowed to meet the drift specification, refer to Section 4.5.

Na < 2mmol/l in 10 minutes at all ranges K & Li < 0.1mmol/l in 10 minutes at 5.00mmol/l

NB In Reference Mode this drift is greatly reduced. Reproducibility (using Peak mode)

< 2.0% CV for 20 consecutive readings of the same bulk sample, aspirating sample for 20 seconds then 10 seconds of air.

Using Peak with Ref (Internal Standard) Mode

< 1.0% CV for 20 consecutive readings of the same bulk sample, aspirating sample for 20 seconds then 10 seconds of air. With a delay of 30 seconds see section 2.9

425 91 002 Issue 9, 6th August 2008 ECN 527

- 23 -

Model 02655-10 & 02655-15 Flame Photometer

Performance Characteristics and Specification

continued

4.5 Warm Up

To achieve the stated specification the flame must be alight for a minimum of 15 minutes, with diluent being aspirated.

4.6 Sample Requirements

Sample Types Serum Na 0 to 199.9mmol/l

(Model 02655-10 and Model 0655-15 incorporate Linearisation in the software to correct for Na self absorption) K 1.00 to 9.99mmol/l Li: 1.0 to 9.9mmol/l

Urine Na 0.0 to 199.9mmol/l

K 40.0 to 120.0mmol/l

These are original sample concentrations before dilution.

Dilution Ratios Na/K 1:200 Serum and Urine

Li 1:50

Type

Is dependent on application. However, samples should not be highly viscous or nonhomogeneous.

Method of Presentation

The diluted sample is presented to the nebuliser from a sample cup, test-tube, or other suitable container. Alternatively a continuous flow diluter, Model 805, can be used and the undiluted sample can be presented to the Dilutor The Model 02655-10 and Model 02655-15 can now be used with the Model 860 Auto-Sampler and Model 805 Diluter giving a fully automatic Clinical System for Na and K analysis.

Volume

The maximum diluted sample volume required for 20 seconds aspiration is 2ml, which gives the minimum sample volume as 10µl. 50µl for the Model 805 and 0.2ml for the Model 860 system.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 24 -

Model 02655-10 & 02655-15 Flame Photometer

Performance Characteristics and Specification

continued

4.7 Chart Recorder Output

Can be adjusted for 100.0 display from 0.05V to 5.0V.

4.8 Environmental Conditions

Temperature

Operating +10°C to +35°C Transportation –10°C to +45°C

The instrument specification will be unaffected by an ambient temperature change of 4°C (or less) per hour, within the range +10°C to +35°C, with a maximum of 7°C shift during 8 hours.

Humidity

Operating: - 85% maximum at +35°C Transportation: - 95% maximum at +45°C (non condensing).

For use in these conditions use the Compressor Model 855 with a water-cooled trap.

4.9 Power Requirements

Voltage

90V to 132V or 198V to 264V, 50/60 Hz

Power

20 VA.

4.10 Fuel

Propane, Butane or Propane/Butane mixture. (Not Natural Gas, as the Model 0265510/15 features a fully automatic ignition system which requires a constant pressure gas supply). All fuels to be free of heavy hydrocarbon deposits and regulated at the cylinder to approximately 2.1kg/cm2 (30psi). NB for the Model 02655-15 Calcium requires Propane/Air flame.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 25 -

Model 02655-10 & 02655-15 Flame Photometer

Performance Characteristics and Specification

continued

4.11 Air

A supply of clean air at a pressure of 0.9kg/cm2 (11psi) at 6 litres/minute, as supplied by a Model 851 Air Compressor. The stability of the readings from the Model 02655-10 and Model 02655-15, especially at high gain, depends on a smooth aspiration of the sample; this can be disturbed by droplets of condensation coming from the air compressor where the compression/expansion causes significant condensation in humid atmospheres. If condensation problems arise a Model 855 Air Compressor should be used, which has a water separator fitted. Air Compressors are listed in Section 9.2.

4.12 Size

Overall, including chimney and rear panel connectors: -

510mm high 390mm wide 345mm deep.

4.13 Weight

9.5kg, instrument only.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 26 -

Model 02655-10 & 02655-15 Flame Photometer

5 Description of Instrument

5.1 Front Panel Controls and Indicators

Model 02655-10/15 Controls

Blank

Channel selector Mode Selector

Measure/Print

Channel 1 Set

Channel 2 Set

5.1.1 Controls

Primary Function Secondary

Function

Reset all channels to zero

Selects single and dual channels for display and printing

Selects Peak, Lithium Reference and Continuous in various combinations Initiates Measure cycle in Peak Mode

Initiates Calibration procedure

Reset instrument to default mode

Initiates Print in Continuous Mode Confirms Calibration Value Confirms Calibration Value

Tertiary Function

Enter set-up mode for Units, Delay, Voltage

Enter set-up mode for Date and Time

Confirms Set-up choices

Enter Set-up Mode for Filter Factor

Quaternary Function

Enter set-up Mode for Filter factor

Software version

‘-’ ‘Set’ ‘+’ (Feedback beep confirms the action)

Primarily used in the calibration process. Secondary functions include: -

date and time setting

Filter Factors and other settings

When Pressed during a measurement session the instrument will automatically interrupt the measurement and require ‘Blanking’ and ‘Calibration’.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 27 -

Model 02655-10 & 02655-15 Flame Photometer

Description of Instrument

continued

Blank

(A feedback beep confirms the action).

Forces the instrument to zero all channels. Must be used at start-up and after change of ‘Peak/Cont’, ‘Single/Dual’ or element selection. A short press may be used at any time during a measurement session to reset the blank while aspirating the blank solution. A long press at any time will reset to default settings and put the instrument into startup blanking mode and into Set-up mode.

Single/Dual and Element Selection

Used at start-up to choose ‘Single’ or ‘Dual’ channel operation. Used to select particular elements by “toggling” through sequence: -

The Model 02655-10 The Model 02655-15

“Na & K” “Na & K”

“Na & Li” “Na & Ca”

“Na” “Na & Li”

“K” “Ca & K”

‘Li’. “Ca & Li” “Na” “Ca” ‘K’ “Li”

When in “Ref” Mode with Li Internal Standard, Li cannot be measured and will not be selectable.

Peak/Ref/Cont

Used at start-up to choose the measurement mode, must be chosen before Blanking. ‘Peak’ has the instrument sense when a steady reading has been achieved and freeze that reading. ‘Ref’ uses signals from the Lithium channel as an internal reference. Can be used with ‘Peak’ or ‘Cont’. ‘Cont’ displays readings in real-time. Sequence is: “Peak & Ref” (Default Mode), “Cont”, “Cont & Ref”, “Peak”.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 28 -

Model 02655-10 & 02655-15 Flame Photometer

Description of Instrument

continued

Measure

(A feedback beep confirms the action).

Used to initiate the measurement and will send an output to the RS232 serial port (connected to a printer, computer or data logger) at the end of the measurement process in “Peak” mode. In ‘Cont’ mode it sends an instantaneous value to the Printer. The sample must be introduced before 'Print' is pressed. Short, long or double presses produce different sample identification in output format (see Section 6.5).

5.1.2 Description of Indicators

All of the front panel indicators are LED’s mounted behind a flexible plastic overlay which provides protection from the laboratory environment. Some windows are plain rectangles others are text legends.

Cal (Ch1 & Ch2)

‘Cal’ on a given channel is illuminated when the instrument automatically goes into calibrate mode after a successful blanking. “Cal” Starts flashing after the Calibration sequence has been initiated ‘Cal’ changes from flashing to steady when either: -

In ‘Cont’ mode. 'Set' is pressed for the second time in the calibration process (see Section 7.2).

In ‘Peak’ mode. The internal part of the calibration process is complete.

Single

Is steadily illuminated when in ‘Single’ (Ch1) or ‘Single’ (Ch2) modes.

Dual

Is steadily illuminated only when in ‘Dual’ mode.

Peak

Indicates that ‘Peak’ mode has been selected.

Ref

Indicates that the ‘Reference’ mode has been selected. ‘Peak’ and ‘Ref’ can both be steadily illuminated at the same time.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 29 -

Model 02655-10 & 02655-15 Flame Photometer

Description of Instrument

continued

Cont

Indicates that ‘Continuous’ measurement mode has been selected. ‘Cont’ and ‘Ref’ can both be steadily illuminated at the same time.

Measure

Indicates that the instrument is in ‘Measurement’ mode. “Measure” flashes during “Peak” measurement process before completion.

Flame On

Indicates that an internal sensor has detected the presence of the flame, absence of illumination after the ignition process will be accompanied by the error message ‘E61’on the measurement displays.

Power On

Indicates that the instrument is on and connected to the proper electricity supply.

5.2 Rear Panel Controls and Connections

Fig: 2.3

425 91 002 Issue 9, 6th August 2008 ECN 527

- 30 -

Model 02655-10 & 02655-15 Flame Photometer

Description of Instrument

continued

Channel 1 & 2 Data Outputs

Both these outputs carry a variable voltage signal (set as indicated in Section 2.10 and

2.11) equivalent to a reading of 199.9 on the two channel displays. When either instrument is the Clinical Mode and when the display is in the lower range for Potassium and Lithium where the display is indicating x.xx the output is equivalent to 19.9. The outputs can be connected to an Analogue/Chart recorder using coaxial plugs (not supplied).

RS232/Printer Output

This is a standard 9way output and the printer output cable (supplied) should be connected here. The other end of the cable is a 9 pin standard RS232 connector for serial printers and other data logging devices.

External Device Output

This is another 8-pin DIN connector, which enables the instrument to be connected to the Model 860 Autosampler.

Power

The instrument is supplied with a Universal External Power supply which can accommodate 90-240V AC input.

Gas

¼-inch fuel inlet connector to the instrument, permanently connected to the in-built regulator inlet (item 5, figure 2.2). The instrument will operate satisfactorily on Propane and Butane.

Air

5/16-inch connector for the air inlet tubing to the instrument (item 2, figure 2.2), from the air compressor outlet. See Section 4.11 for pressure and flow rate specifications.

Air Regulator

Control to adjust the pressure of the air supply (item 3, figure 2.2). The air pressure gauge provides a visual indication of the air pressure.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 31 -

Model 02655-10 & 02655-15 Flame Photometer

Description of Instrument

continued

5.3 Operating Modes

Operating Modes for Model 02655-15 are additional and in italics.

5.3.1 Single/Dual Mode

Refer to Section 5.1.1 for the function of the 'Single/Dual' button and to Section 5.1.2 for its status indicators ‘Single’, ‘Dual’.

Single

The Model 02655-10 has 3 detectors one each for Na, K and Li, permanently producing signals from the light of the flame. The option of running in single channel mode has been provided and is selected by use of the 'Single/Dual' button mentioned above. This allows the user to choose not only between ‘Single’ or ‘Dual’ operation but also between Ch1 and Ch2. to access K or Li.

For the Model 02655-15 there are 4 detectors the additional detector is for Calcium, which can be selected as a single element.

Dual

This mode allows the user to simultaneously measure two elements in the same sample. For clinical use this guarantees better than a 100% time saving compared with the Model 02655-00 single channel instrument.

Ch1 will display Na. Ch2 can be either K or Li unless in Ref. mode when Li is used as the reference element and cannot also be displayed.

Calcium in combination with Na will be shown on the lower display. When in combination with K (or Li when not in “ref” mode) it is in the upper display.

Blanking

Affects all channels so it will operate in the same way regardless of which of these modes has been selected.

Calibration

If operated in Dual mode, both channels must be calibrated before measurements can begin, but it does not matter which channel is calibrated first.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 32 -

Model 02655-10 & 02655-15 Flame Photometer

Description of Instrument

continued

5.3 Operating Modes continued

5.3.2 Peak/Cont

Refer to Section 5.1.1 for the function of the ‘Peak/Cont’ button and to Section 5.1.2 for its status indicators ‘Peak/Ref/Cont’.

Peak

This mode is intended to give the user a frozen measurement value on the instrument display. This not only removes subjectivity form the user but also offers a much greater advantage by enabling automation of sample delivery and collection of results.

The Peak Cycle

The full sequence of the peak process is as follows: The cycle commences with the introduction of a new sample, quickly followed by pressing 'Measure'. The instrument starts a built-in delay (which can be varied see Section 2.9) as soon as a press of 'Measure' has been registered, (whether a short, long or double press does not matter); readings are displayed on all operative channels. When the values satisfy the peak detection algorithm the display(s) freeze and the "Measure" indicator will become steady. A single beep will sound and a valid measurement value will be output to the RS232 serial port.

The cycle cannot re-commence until the previous values flash and the measure indicator is steady. Then a new sample can be aspirated and “Measure” is pressed. If the cycle has timed out for the previous sample before the algorithm was successful on all operative channels, "measure" will flash and "E22" will be showing on at least one display.

(NB a full list of all codes is printed as Appendix B).

425 91 002 Issue 9, 6th August 2008 ECN 527

- 33 -

Model 02655-10 & 02655-15 Flame Photometer

Description of Instrument

continued

5.3 Operating Modes continued

5.3.3 Ref Mode

Ref. stands for internal reference. This mode uses the signal from the Li detector to scale the readings from the other detector(s) to allow for external effects such as flame disturbances, changes in dilution, nebuliser uptake rate, etc. For full explanation see Section 3.3.

To use ‘Ref’ mode make sure Li is not selected and the calibration and measure samples will have to be in a solution with the known concentration (we recommend 100ppm Li), C0, of Li in the diluent. Make sure that calibration and measurement samples contain the same concentration of Li.

Cont

‘Cont.’ is short for continuous. Readings are continually updated. There are no timeouts or delays. This mode can be used in various combinations with Single, Dual and Ref. There is one major difference from the Peak mode, a press of 'Print' causes output of the current displayed reading to whatever is connected to the RS232 connector, e.g. printer, computer, data logger.

5.3.4 Blank

Blanking can be initiated at any time during a measurement session by pressing 'Blank', having already introduced a blank sample. There are 5 circumstances where it is initiated automatically by the instrument as part of the start-up sequence: On initial Switch on after Diagnostic sequence

immediately after use of 'Peak./Cont' immediately after use of 'Single/Dual' immediately after any element selection process immediately after 'Set' is pressed during a measurement session

NB It may be that more than one of the above buttons is pressed as the user selects a new operating mode. "000" flashing in the measurement displays will indicate that the instrument is waiting for the user to press 'Blank' and the blanking process will not commence until it is pressed.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 34 -

Model 02655-10 & 02655-15 Flame Photometer

6 Operating the

Instrument

6.1 Start Up

After completing the assembly of the instrument as described in Chapter 2 (remembering to fill the ’U’ tube with water and to allow the waste water to fall to a reservoir) the instrument is ready to be started.

6.1.1 Compressor Start Up

Switch on the compressor and adjust the air regulator assembly (see Section 5.2) to read 11psi on the gauge.

6.1.2 Switch On

The Model 02655-10 and Model 02655-15 automatically select a high gas /air ratio to ignite the flame. If the instrument has been switched off recently then the flame should ignite at the first operation of the 1/0 switch. If not then two or even three operations might be necessary to fill the gas tube up-stream of the instrument.

6.1.3 Ignition Successful

The instrument automatically selects a reduced gas/air mixture, which optimises the flame for highest sensitivity. The Flame detector operates and the “flame on” light illuminates.

6.2.1 Required Blanking

When the instrument has just been switched on it automatically goes into blanking mode as soon as the flame has lit (although this may be temporarily interrupted by the date and time setting and other processes described in Section 2.7 to 2.9). At this point: "Measure" and "Cal" are off, the display(s) flash "000"and the instrument waits for the user to introduce the blank sample before pressing 'Blank'. ‘Blank’ affects all channels simultaneously, even Li whether being used for reference or not. The instrument brings the readings of all channels to zero, a steady display of "0.0" on all enabled channels.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 35 -

Model 02655-10 & 02655-15 Flame Photometer

Operating the Instrument

continued

6.2.2 Required Calibration

(NB Li must be in diluent if operating in Ref). On initial start up the instrument requires a Calibration step after the Blanking process. The instrument goes into calibrate mode with the "Cal"(s) flashing, "Measure" off, and the most recent calibration values on the display. The ‘Single’ and ‘Dual’ mode of operations both Calibrate in the same manner. The preparation and presentation of standards in “Dual” are different for “Cont” and “Peak”.

Continuous Mode.

(In Dual Mode standards can be presented as individual elements and each channel separately calibrated). The channel(s) should be adjusted to desired value(s) using '-' or '+'. When desired value(s) are flashing introduce the standard and then press 'Set' in any order within 2 seconds of each other. The user waits for readings to reach what the user judges to be steady plateau values and presses 'Set' on each channel at the appropriate time to confirm the values. As these presses of 'Set' are registered the displayed readings are adjusted to calibration values, output is sent and the instrument goes into measure mode with "Measure" on and "Cal" off.

Peak Mode (With Reference is Default Mode).

(In Dual Mode both Sodium and Potassium standards must be mixed and presented as one solution as there is no operator involvement in the calibration sequence). Each channel should be adjusted to desired value using '-' or '+'. When desired value is achieved press “Set” on that channel and then modify the other channel if necessary and introduce the calibration sample IMMEDIATELY BEFORE pressing 'Set' on the second channel. The “Peak” process starts on both channels and readings are displayed. Once the Peak algorithm has accepted the values, and the displays on both channels change to the calibrated values, the user presses 'Set' on both channels and the output is sent. The displays start flashing, "Cal" goes out, "Measure" comes on and the instrument is ready to measure.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 36 -

Model 02655-10 & 02655-15 Flame Photometer

Operating the Instrument

continued

6.3 Sample Measuring

6.3.1 Measuring in Peak mode

The cycle commences with the introduction of a new sample, quickly followed by pressing 'Measure'. The instrument starts the built-in delay (which can be varied see Section 2.9) as soon as a press of 'Measure' has been registered (whether a short, long or double press does not matter). Readings are displayed on all operative channels and ‘measure’ is flashing. Once the peak mode algorithm is satisfied, the values will “freeze”, the signals are corrected to the reference signal, “measure” will become steady and a valid measurement value will be output to the RS232 serial port. After 5 seconds the Display(s) will flash prompting the user to remove the sample, introduce a new sample and press 'Measure' to start the cycle again.

6.3.2 Measuring in Continuous mode

In Continuous Mode, after introducing the sample, the user will decide when the displayed results have stabilised and the reading can be taken and/or sent to the printer/output. When he/she is so satisfied then a simple press of the ‘Measure/Print’ button will send the currently displayed/measured value to the output port. A new sample can be presented at any time and the user again can take the result when it is judged ready.

6.4 Automatic operation

Both the Model 02655-10 and Model 02655-15 can be easily used in a partial or fully automatic system using the Model 805 Continuous Flow Diluter and the Sherwood Model 860 Autosampler. The Model 805 diluter is specifically designed to work with either system for Clinical samples, especially when used in “Ref” Mode where the Lithium Internal standard is diluted using the Model 805, will give excellent drift free reproducibility.

The Model 860 Autosampler is used as a slave to the Flame Photometers and operates the Blank and Calibration and Measure cycle under the commands already described in Sections 6.2 and 6.3 above.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 37 -

Model 02655-10 & 02655-15 Flame Photometer

Operating the Instrument

continued

6.5 Sample Numbering

The Sample number is set at 001 when the initial calibration is successful and the “measure” light is illuminated. The sample number is then incremented at each single press of the ‘Measure’ button both in “Cont” and “Peak” modes.

6.5.1 Reset of sample number

The sample number is reset to 001 when a calibration is carried out or if both “+” s are pressed simultaneously.

6.5.2 Repeat sample

If it is necessary to repeat a sample so that the new value is listed against the same sample number then the “Measure’ button is pushed twice; the output is RRR instead of the next sample number.

6.5.3 QC sample

A Quality Control sample can be identified by a long push on the print button: the output is then QQQ and the next sample is not incremented.

To confirm that the instrument has accepted these different pushes on the “Measure” button the measurement display will disappear for two seconds during which time the second push will be accepted.

6.6 Shutdown Procedure

1 Aspirate Cleaning Solution diluted 1 in 100 with distilled water, for one minute.

2. Aspirate diluent for two minutes.

3. For a short-term shutdown (two hours) switch off the instrument at the power switch and switch off the compressor.

4. For a longer term shutdown (overnight) turn off the fuel supply at source. When the flame on LED is extinguished, switch off the power switch, and the compressor. This ensures that the fuel pressure in the fuel tubing is at a minimum.

NB Remember that the Instrument will remain HOT for a

considerable time after switch off.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 38 -

Model 02655-10 & 02655-15 Flame Photometer

7 Operating Hints, Precautions & Limitations

7.1 Hints

1. The high quality distilled water used when making a batch of diluent must be free from contaminating elements. It is recommended that the same batch of diluent be used to prepare all solutions and to set ‘blank’ on the instrument.

2. Greatest accuracy will be obtained by using the same dilution equipment for both standard and sample preparation.

3. Always use suitable standards for calibrating the instrument. Remember that the accuracy of the results obtained from the any Flame Photometer depend on the accuracy and purity of the calibration standard that is used.

4. A ‘Guide to Flame Photometry’ containing Applications and Method Sheets is available from your Cole-Parmer Distributor.

7.2 Operational Precautions and Limitations

1. Always dilute samples and standards with the same batch of diluent (made up of 1 part Diluent Concentrate and 999 parts of good quality distilled water), which contains non-ionic wetting agents.

2. The samples should not be highly viscous or non-homogeneous. If possible, samples likely to contain sediment should be filtered and then mixed to obtain a representative result.

3. Always use soap solution when checking for leaks in fuel or air lines. Do not allow fuel to flow in the presence of unguarded flames, e.g. cigarettes.

4. Always use genuine Cole-Parmer replacement parts. Do not, for example, replace the 'U' tube with one of different material, bore or length as this will cause a deterioration in the instrument's performance.

5. Always carry out the maintenance schedules as detailed in Section 8.

6. Do not leave the inspection flap open, unless adjusting flame conditions, as this will allow stray light to enter the chimney.

7. The front panel of the M02655-10/15 is impervious to a wide range of chemicals. However, strong acids and some organic solvents e.g. Chloroform and Phenol may affect the finish. Any spillage should be thoroughly wiped away as soon as possible. If necessary, clean the instrument with warm, soapy water do not use abrasives.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 39 -

Model 02655-10 & 02655-15 Flame Photometer

Operating Hints, Precautions and Limitations

continued

7.3 Hazards

1. All electrical instruments are potentially hazardous. With the exception of the glass chimney, there are no user maintainable parts inside the Model 0265510/15 covers. Never remove covers from the instrument, unless specific maintenance instructions are being followed.

2. Propane and Butane are highly inflammable and potentially explosive gases. Propane and Butane are stored as a liquid, under pressure in a cylinder, for use with the instrument. Such a cylinder should never be subjected to heat or mechanical shock. When handled correctly and connected to the instrument as instructed, the fuel gas is quite safe. Check hosing joints with a soap solution before allowing any naked flame in the vicinity. Never open a cylinder valve to atmosphere even on a supposedly empty cylinder.

3. The chimney cap and glass chimney and the area above the chimney can

become very hot and are capable of causing severe burns. Never view the flame from the top of the chimney, always use the inspection hole.

4. Make sure that the air compressor is connected to the power supply and switched on before starting a flame ignition sequence. If this is not done the instrument will not light, as there is a safety cut out which detects the air pressure. If there is no air pressure, error code ‘E61’ will be displayed ‘No Flame’.

7.4 Measuring Calcium

The Model 02655-15 has the additional channel for Calcium, which has been requested by many customers. It should be noted however that because the Model 02655-15 uses propane /air flame there is not sufficient energy to break the Calcium Phosphate bond which may occur within, particularly, clinical samples. In these samples a pretreatment protocol must be used.

With other Calcium samples Chelation with EDTA or extraction with Mehling’s 3 solution may be indicated.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 40 -

Model 02655-10 & 02655-15 Flame Photometer

8 Cleaning and Maintenance Procedures

8.1 General

Under the Daily, Weekly, Monthly and Six-Monthly Maintenance headings are summaries of the work and equipment required. The tasks are detailed from Section 8.6 onwards. For maintenance of the Air Compressor and Dilutor, if used, refer to the instructions supplied with the equipment.

8.2 Daily Maintenance

EQUIPMENT REQUIRED: None.

1. Empty waste container, if used.

2. Check air line for condensation, and drain if necessary.

3. Check 'U' tube is filled with water.

8.3 Weekly Maintenance

EQUIPMENT REQUIRED: 10ml beaker Stopwatch Nebuliser cleaning wire Deproteinising Solution Stainless steel nebuliser tube and sleeve, or polythene inlet tube, as required.

1. Carry out Daily Maintenance procedure.

2. Deproteinise the system (see Section 8.10).

3. Check the operation of the nebuliser (see Section 8.6).

8.4 Monthly Maintenance

EQUIPMENT REQUIRED: As for weekly maintenance.

1. Carry out Daily and Weekly Maintenance procedures.

2. Check the constant head and drain, mixing chamber, 'U' tube and drain tube and clean if necessary (see Section 8.7). Check that the 'U' tube is refilled with water on re-assembly.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 41 -

Model 02655-10 & 02655-15 Flame Photometer

Cleaning and Maintenance Procedures

continued

8.5 Six Monthly Maintenance

EQUIPMENT REQUIRED: As for weekly maintenance, plus the following: 'U' tube and drain tube Methanol Tissues Soft lint free cloth Cotton buds

1. Carry out the Daily and Weekly Maintenance procedures.

2. Clean the mixing chamber, burner tube, burner and constant head and drain (see Section 8.7).

3. Replace polythene nebuliser tube, 'U' tube and drain tube.

4. Clean the optical filters and the glass chimney (see Section 8.8).

5. Check the air and fuel tubing and connectors for leaks, using soap solution.

8.6 Nebuliser

EQUIPMENT REQUIRED: Stopwatch 10ml beaker Cleaning wire Cleaning Solution Polythene inlet tube

8.6.1 Operational Check

1. Turn off the fuel supply at source.

2. Switch on the instrument and the air compressor. Check that the ‘flame on’ LED is off.

3. Fill the beaker with deionised water, and weigh it.

4. Present the 10ml beaker of deionised water to the nebuliser tube for an accurately timed minute.

5. Re-weigh the beaker and calculate the aspiration rate. If it is between 2 and 6g/minute, no further action is required. If it is too low, continue with paragraph 6. If it is too high contact your Sherwood Scientific Distributor.

Do not attempt to adjust the nebuliser as the capillary position is fixed during manufacture, and is not adjustable.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 42 -

Model 02655-10 & 02655-15 Flame Photometer

Cleaning and Maintenance Procedures

continued

8.6 Nebuliser, continued

8.6.2 Cleaning the Nebuliser

6. Release the nebuliser retainer (item 2, figure 2.1) and withdraw the nebuliser (item 1, figure 2.1).

7. Insert a cleaning wire into the nebuliser capillary.

8. Refit the nebuliser inlet tubing to the nebuliser. Recheck the aspiration rate, paragraphs 3, 4, and 5.

9. If nebuliser operation is still outside the specification, unscrew the air line connector and remove the nebuliser inlet tubing.

10. Soak the nebuliser in a 1 in 100 dilution of Cleaning Solution, agitating it periodically.

11. Rinse thoroughly in deionised water and shake dry.

12. Refit the nebuliser inlet tubing and the air line. Re-check aspiration rate.

13. If the nebuliser operation is still unsatisfactory, fit a new nebuliser.

8.7 Cleaning the Mixing Chamber, Burner and Drain

EQUIPMENT REQUIRED: Flat blade screwdriver, blade width 8 mm Deproteinising Solution Tissues

1. Check that the flame on LED is off, fuel is turned off at source, and the instrument and compressor are switched off.

2. Release the nebuliser retainer and withdraw the nebuliser (item 1 and 2, figure

2.1).

3. Disconnect the fuel tubing (item 5, figure 2.1) from the end cap connector (item 6, figure 2.1) using a twisting movement. Do not pull the tubing, as this will make it grip the connector more tightly.

WARNING Use a heat resistant glove when handling the chimney, burner and burner tube if the flame has been alight within the preceding 30 minutes.

4. Remove the chimney cover and lift out the glass chimney.

5. Disconnect the mixing chamber from the chimney assembly by twisting it to the right, through 40 degrees. Lower the mixing chamber and burner assembly.

6. Lift the burner tube from the mixing chamber and remove the burner.

7. Unscrew the end cap securing screw (item 7, figure 2.1). Grasp the end cap (item 6, figure 2.1) and remove it from the mixing chamber, with a slight twisting action. Lift the end cap to allow the water in the 'U' tube to flow into the constant head and drain. Disconnect the 'U' tube from the end cap and constant head and drain.

8. Unclip the constant head and drain and disconnect the drain tube.

9. Soak all the items removed from the instrument in a 1 in 100 dilution of Cleaning Solution, agitating it periodically.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 43 -

Model 02655-10 & 02655-15 Flame Photometer

Cleaning and Maintenance Procedures

continued

8.7 Cleaning the Mixing Chamber, Burner and Drain continued

10. Rinse the parts thoroughly in distilled water and dry with clean tissues.

11. Fit the burner tube to the mixing chamber, ensuring that the locating slot in the burner tube engages with the locating pin in the mixing chamber.

12. Fit the burner pointed end downwards into the burner tube. Rotate the burner to ensure that it is fully inserted.

13. Check the condition of the end cap '0' ring seal. If necessary, replace the seal.

14. Replace the end cap, using a twisting movement. Align the hole with the mixing chamber screw fixing. Fit the screw (item 7, figure 2.1).

15. Carefully position the mixing chamber, with the burner tube inside the chimney. With the burner tube fully inserted, twist the mixing chamber to the left, through 40 degrees, to lock it in position.

16 Fit the constant head and drain to the spring clip and position it so that the lip

on the drain is resting on the spring clip.

17. Connect the fuel tubing to the mixing chamber end cap. Connect the 'U' tube between the mixing chamber end cap and the constant head and drain. Fit the drain tube to the constant head and drain.

18. Fit the nebuliser into the end cap and position the retainer to lock it.

19. Use a wash bottle to completely fill the 'U' tube with water.

8.8 Cleaning the Glass Chimney

EQUIPMENT REQUIRED: Soft lint free cloth Methanol Cotton buds Tissues Cleaning Solution

1. Check that the ‘flame on’ LED is off, fuel is turned off at source, and the instrument and compressor are switched off.

WARNING Do not proceed until all the parts within the chimney are at a safe handling temperature.

2. Lift off the chimney cap from the chimney assembly. Lift out the glass chimney.

3. Soak the glass chimney in a 1 in 100 dilution of Cleaning Solution, agitating it periodically.

4. Rinse the glass chimney thoroughly in deionised water and dry with clean tissues.

5. Replace the glass chimney with the clear strip at the bottom. Make sure the glass chimney is seated correctly and re-fit the chimney cap on top of the chimney.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 44 -

Model 02655-10 & 02655-15 Flame Photometer

Cleaning and Maintenance Procedures

continued

8.10 Deproteinising or Disinfecting Procedure

NOTE To deproteinise the system use Deproteinising Solution, to disinfect the system use Tubing Disinfectant.

EQUIPMENT REQUIRED: Deproteinising Solution or Tubing Disinfectant.

1. Light the flame as detailed in Section 5.4.

2. Present a beaker of Deproteinising Solution or Tubing Disinfectant to the nebuliser for 10 minutes.

3. After 10 minutes have elapsed, replace the beaker of solution with a beaker of deionised water. Flush the system for two minutes.

4. Shutdown the instrument as detailed in Section 6.6.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 45 -

Model 02655-10 & 02655-15 Flame Photometer

9 Accessories, Consumables & Spare Parts

9.1 Ordering Information

When ordering spares or accessories for your instrument, please give the following information to your Cole-Parmer distributor.

Instrument Serial Number Catalogue Number of Part (Cat. No.) Description Quantity required

This will ensure that your order is dealt with quickly and efficiently. The number shown in the third column (Quantity) is the quantity of items that are supplied against the stated Catalogue Number. If the quantity is greater than 1, then only multiples of that quantity can be supplied.

9.2 Spares and Accessories

Spares

Catalogue # Description Quantity

420 08 102 Nebuliser 1 410 26 001 Mixing chamber and burner 1

402 21 001 End Cap Assembly 1 400 02 013 End cap securing screw 1 410 04 010 Constant Head and Drain 1 400 22 003 Nebuliser inlet tube, polythene, 150mm each 1 400 22 012 Nebuliser inlet tube, polythene, 150mm Pk 12 1 420 09 022 Fuel restrictor 22 g 1 420 09 023 Fuel restrictor 23 g 1 001 26 033 Sample pot, plastic, in packs of 50 1 pack 420 27 137 Sodium filter, Factored 1 420 27 138 Potassium filter, Factored 1 420 27 139 Lithium filter, Factored 1 420 27 140 Calcium filter, Factored 001 08 234 Propane primary regulator 1 001 08 439 Butane primary regulator for 7kg Calor/Caravan cylinder 1 001 72 114 Fuel tubing, reinforced per meter 001 72 025 Gas tubing (internal) per meter 001 08 718 ‘Unex’ tubing clamp 1 100 99 010 Nebuliser cleaning wire, pack of 3 1 pack 926 09 052 RS232 Output Cable 1

425 91 002 Issue 9, 6th August 2008 ECN 527

- 46 -

Model 02655-10 & 02655-15 Flame Photometer

Accessories Components and Spare Parts

continued

Accessories

473 56 903 Printer assembly, universal 1 475 41 100 Model 805 Continuous Flow Diluter for Clinical Samples 1:200

Ratio 860 00 009 Model 860 Autosampler 40 position 1 851 01 001 Model 851 Air Compressor 1 855 01 001 Model 855Air Compressor, complete with water separator 1

9.3 Reagents

Catalogue # Description Quantity

001 56 100 140.0mmol/Na, 5.00mmol K, 1.50mmol/L Li, 100ml 1 Bottle

NOTE: The following solutions are also available

001 56 620 Flame Photometer Standard, 1000ppm Na, 6 x 100ml 1 Pack 001 56 621 Flame Photometer Standard, 1000ppm K, 6 x 100ml 1 Pack 001 56 622 Flame Photometer Standard, 1000ppm Li, 6 x 100ml 1 Pack 001 56 623 Flame Photometer Standard, 1000ppm Ca, 6 x 100ml 1 Pack 001 56 681 Diluent Concentrate, 6 x 100ml 1 Pack 001 56 682 Tubing Disinfectant, 6 x 100ml 1 Pack 001 56 183 Deproteinising Solution, 6 x 80ml 1 Pack

425 91 002 Issue 9, 6th August 2008 ECN 527

- 47 -

Model 02655-10 & 02655-15 Flame Photometer

Appendix A

Bibliography

Reference

1. Kolthoff, I.M., Z. Anal. Chem. 70 397 1927

2. Shohl, A.T., and Bennett, H.B. J. Biol. Chem. 78: 643

1928

3. Hald, P.M., J. Biol. Chem. 167. 499. 1947 4 Bernstein, R.E., S.Afr. J. Med. Sci. 17. 101, 1952

5. Alkemade, C.T.J., Smit J. and Verschure J.C.M.

Biochimica et Biophysica Acta. 8. 562. 1952

6. Collins G.C. and Polkinthorne H., The Analyst, 77,

917 pp. 430-436, 1952

7. Dryer, R.L., Clin. Chem. 2. 112. 1956

8. Puffeles, M. and Nessim, N.E., The Analyst. 82. 976,

1957

9. Boling, E.A., J. Lab and Clin. Med. 63, 501 1964

10. Amdisen, A., Scand J. Clin. and Lab. Invest. 20 (2)

104. 1967

425 91 002 Issue 9, 6th August 2008 ECN 527

- 48 -

Model 02655-10 & 02655-15 Flame Photometer

Appendix B

Error

Message

Possible causes

Things to try

Way out for user

E02

E05

E 06 Detector circuit saturated

E09 Blank out of limits. (E09

E12

E15

Blanking timed out.

Blank signal outside

offset range.

in Blank.

is displayed on the

offending channel(s) and

the appropriate element

symbol(s) will flash).

Peak cycle timed out

during calibration.

Calibration Invalid. Reading/Calibration Factor

Sample ran out. Noisy

signal due to sample. Noisy

signal due to instrument

malfunction.

Blank contaminated.

Gross contamination of

blank; Blank not being used.

There are arbitrary ceilings

for blank signals on each

element.

Sample was not a blank.

Signal was too noisy.

Li in blank when it shouldn't

have been.

Sample ran out. Noisy

signal due to sample. Noisy

signal due to instrument

malfunction.

>99.

Check there is plenty of

sample. Verify nebuliser

uptake rate.

Check with fresh blank.

Try a guaranteed blank.

Check the atomiser.

Check there is plenty of

sample. Verify nebuliser

uptake rate.

Check concentration of

Standard has it been

diluted correctly.

E02 is displayed for 5s, then automatically returns to start

of blanking. No output sent.

Re-start Blank.

E09 is displayed for 5s, then automatically returns to start

of blanking. Make sure a

blank sample is being

aspirated and press "Blank".

Output will also indicate

error by showing

"----" for measurement

value(s).

E12 is displayed for 5s, then automatically returns to start

of calibrate

(does not go back to

beginning of blanking). No

output sent.

Start Calibration sequence

again.

E16 Detector circuit saturated

in Calibrate.

E19

E22

Standard reading over-

range.

(In calibrate only).

Peak timed out during

measure.

Gross error in Standard

concentration; Standard not

diluted.

There are arbitrary ceilings

for signals on each element.

Wrong value Standard. Signal was too noisy. Li in Standard when it shouldn't

have been.

Sample ran out. Noisy

signal due to sample. Noisy

signal due to instrument

malfunction.

standard. Increase sample

Check there is plenty of

sample. Verify nebuliser

425 91 002 Issue 9, 6th August 2008 ECN 527

Check concentration of

Standard has it been

diluted correctly.

Check concentration of

dilution.

uptake rate.

Start Calibration sequence

again.

In CONT. E19 is displayed

until something causes

reading to come back in

range. In PEAK E19 is

displayed until cancelled by

introduction of new standard

and press of 'Set'.

Press 'Print' in usual way. (To

repeat without changing

sample number press and

hold 'Print') Output will also

indicate error by showing "---

-" for measurement value(s).

- 49 -

Model 02655-10 & 02655-15 Flame Photometer

Error

E25

E26 Detector circuit saturated

E29

E30 Li signal outside limits in

E35

Message

Sample > 125% of top

standard.

in Measure mode.

Sample reading over-

range.

(In measure only)

(Output shows ***.* for

value).

blanking. (In Ref. mode only). (E30 displayed on Ch2 and "Ref." flashes).

Li out of range in

measure + Ref. mode.

(E35 displayed on active

channels and "Ref."

flashes).

Possible causes

Sample not diluted

correctly. Very abnormal

sample; incorrect standard

used for sample.

Sample not diluted

correctly.

Maximum reading that

display can produce is

"199.9".

Li Concentration in the final

Blank solution should be

100ppm.

Limits on the excursions of

the Li signal in ref. mode

are ± 20% about the value

at calibration. Either Li has

run out or there is a fault

with the instrument or

dilutor.

Things to try

Check correct standard;

check dilution, if necessary

increase dilution ratio.

Check dilution of sample. Re introduce correctly diluted

Check concentration of

sample. Increase sample

dilution.

Check source of Li. Check dilutor and tubing. Check

nebuliser.

Check source of Li. Check dilutor and tubing. Check

nebuliser.

Way out for user

In CONT. message will

disappear when sample

removed; in PEAK introduce

new sample and press Print.

sample.

In CONT. E29 is displayed

until something causes

reading to fall below "199.9".

In PEAK E29 is displayed

until cancelled by

introduction of new sample

and press of 'Print'.

E30 is displayed for 5s, then automatically returns to start

of blanking. No output sent.

E35 is displayed on active

channels and "Ref." flashes

for 5s, then automatically

returns to start of blanking.

Aspirate a blank solution and

press 'Blank'.

E39 Li signal outside limits in

calibrate. (In Ref. mode

only). (E39 is displayed

on Ch2 and "Ref."

flashes).

E61 Flame had not ignited by

the end of the ignite

cycle.

E62

E68

E69

Battery Back up Failure

on Power On.

Serial Interface not

responding.

Microprocessor not

responding.

Li Concentration in the final

Standard solution should be

100ppm.

No gas from supply; gas

leak; no water in constant

head tube; air pressure low

or zero (compressor

problem, wrong setting of

regulator or leak).

Circuit Fault or Battery

fault.

Serial device not switched

on or faulty.

Temporary electronic

transient.

Check concentration of

sample. Increase sample

dilution.

Check that there is gas and

that it is connected; check

that the constant head tube

is full; check there is air,

that it is connected and

that the pressure showing

on the gauge is correct.

Can reset Filter Factors.

See Manual.

Switch on device or

replace.

Switch Off and On again

after 15 second delay.

E39 is displayed for 5s, then automatically returns to start

of calibrate (does not go back

to beginning of blanking).

Ensure diluent has correct Li

concentration.

Switch off, wait a moment

then switch on again.

Press "Blank" to continue.

Have to reset Standard

Values each time user

calibrates.

Remove faulty device, which

will remove error code.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 50 -

Model 02655-10 & 02655-15 Flame Photometer

Appendix C

Using the Zero offset and Span control in “301” Mode for Model 425

This mode was introduced to improve the flexibility of interfacing the Model 425 with other analytical equipment. It allows the normal calibration routine to be bypassed and is more suitable for continuous flow analysers.

Setup

1 Reset the Model 425 by pressing and holding down the “Blank” control until

the display shows “300”, (following the sequence 2.8-2.10 in the Operator

Manual).

2 Change the setting to “301”. Press ‘Set’. 3 “401” is displayed this allows the damping to be changed see (section 2.11 in

Operator manual). Press ‘Set’.

4 Display reverts to the blank setting “000” flashing on each channel.

Operation of Zero Offset

1 Aspirate blank solution and press “Blank”. 2 When blank is “captured” after 5 seconds the “Cal” lights flash. 3 Operate the “+” or “-“ keys to change the output analogue voltage. 4 Press “Set”.

Operation of the Span

1 “Std” will be displayed. Aspirate the appropriate standard (e.g.10.0 ppm Na.

This should be greater than the maximum concentration expected from samples

to ensure that the samples appear on scale at the analogue output.)

2 Whilst aspirating the standard change the “+” or “-“ controls to change the

span setting on the analogue voltage. Press “Set”.

Measure

Having set the Zero offset and span, all samples should now fall within these two analogue voltages.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 51 -

Model 02655-10 & 02655-15 Flame Photometer

Cole Parmer Instrument Company Product Warranty Statement

_________________________________________________________________________________________

Warranty Term: 12 Months

Cole Parmer Instrument Company (Cole Parmer) warrants, subject to the conditions itemized within this document, through either Cole Parmer personnel or personnel of its authorized distributors, to repair or replace free of all charges, including labor, any part of this product which fails within the warranty time specified above, appertaining to this particular product. Such failure must have occurred because of a defect in material or workmanship and not have occurred as a result of operation of the product other than in accordance with procedures described in the instructions furnished with this product.

Conditions and specific exceptions that apply to the above statement are as follows:

1. End-user warranty time commences on the date of the delivery of product to end-user premises.

2. ‘Free of all charges’ statement applies only in areas recognized by Cole Parmer as being serviced either directly by its own personnel, or indirectly through personnel of an authorized distributor. Products purchased outside these areas requiring service during the warranty period will incur charges relative to the travel/transit costs involved. However, products purchased in such areas will be serviced during the warranty period free of all charges providing they are returned, carriage paid, to either Cole Parmer or by pre-arrangement to an authorized Cole Parmer distributor.

3. All maintenance (other than operator maintenance as described in the instructions), repairs or modifications have been made by Cole Parmer or Cole Parmer authorized personnel.

4. This product has where applicable been operated using Cole Parmer specified supplies and reagents.

5. Cole Parmer reserves the right to make any changes in the design or construction of future products of this type at any time, without incurring any obligation to make any changes whatsoever to this particular product.

6. Reagents, supplies, consumables, accessories and user maintenance items are not included in this warranty.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 52 -

Model 02655-10 & 02655-15 Flame Photometer

Product Warranty Statement (continued)

7. Repairs or replacement of any part failing due to abnormal conditions including the following, are excluded from this warranty:

a. Flood, lightning, earthquake, tornado, hurricane, or any other natural or

man-made disaster.

b. Fire, bombing, armed conflict, malicious mischief or sprinkler damage. c. Physical abuse, misuse, sabotage or electrical surge. d. Damage incurred in moving the product to another location.

8. User agrees to permit Cole Parmer personnel or personnel of its authorized distributor to make changes in the product which do not affect results obtained, but do improve product reliability.

Representations and warranties purporting to be on behalf of Cole Parmer made by any person, including distributors and representatives of Cole Parmer, which are inconsistent or in conflict with the terms of this warranty (including but not limited to the limitations of the liability of Cole Parmer as set forth above), shall not be binding upon Cole Parmer unless reduced to writing and approved by an officer of Cole Parmer Instrument Company.

Except for the obligations specifically set forth in this warranty statement, in no event shall Cole Parmer be liable for any direct, indirect, special, incidental, or consequential damages, whether based on contract, tort or any other legal theory and whether advised of the possibility of such damages.

Neither Cole Parmer nor any of its third party suppliers makes any other warranty of any kind, whether expressed or implied, with respect to Cole Parmer Products.

Cole Parmer Instrument Company, 625 East Bunker Court, Vernon Hills, IL 60061, USA.

425 91 002 Issue 9, 6th August 2008 ECN 527

- 53 -

Cole-Parmer 02655-10, 02655-15 Operator Manual

Specifications and Main Features

Frequently Asked Questions

User Manual