C&L Instruments VC-LFR-25, VC-LFR-18-H, VC-CPR-25, VC-OTR-25, VC-LFR-18 User Manual

TM
Vacu-Cell
Incubation Chamber System*
C&L introduces the Vacu-Cell line of cell incubation chambers. This unique
chamber design holds a coverslip against the chamber body by one side only,
providing major advantages over other types of cell chambers. Several
models are available to fit your specific application.
Advantages of the Vacu-Cell:
- Complete access to the bottom of the chamber, all the way to the edges. Designed for cell and surface microscopy, these chambers are perfect for oil immersion objectives and TIRF experiments when access across the entire coverslip is desired.
- A chamber is formed very simply and quickly, with no snaps, screws or fixtures. Coverslips do not break!
- There are no parts to lose or break.
- Many configurations and styles are available, including open top and laminar flow configurations.
- The chamber is formed directly from the coverslip, Petri dish or microtitre plate by suction.
*Patent Pending
C&L Instruments, Inc.
717-564-9491
Anatomy of a Vacu-Cell
TM
- Round Series
The unique feature of all Vacu-Cell incubation chambers is the use of a vacuum generated force to hold the optical surface tightly against to the body of the chamber. Dual o-rings are used to separate the evacuated space from the remainder of the chamber. Several types of chamber configurations are available, including chambers for circular and rectangular coverslips. Chambers are formed quickly and easily without the need for screws, nuts or retaining rings. This specification sheet describes the Round Series of chambers. Visit our website for decriptions of other series.
Inlet and outlet tubes can be either 1/16 (standard) or 1/8 in. OD. Tubes are available in steel or plastic, and in a variety of ID's.
The vacuum port connects to 1/16 in. ID tubing
Several flow patterns are available. The flow through this laminar perfusion chamber is shown by arrows.
Connectors are standard 1/4-28 in. fittings, that can accept either 1/16 or 1/8 in. OD tubing. Tubing is held securely with a ferrule. Hose barb connections for 1/16 in. ID tubing are available as an option.
Most chamber bodies are
available in several materials, including aluminum, stainless steel, black delrin and clear acrylic.
Most models are available with an integrated heating element for precise temperature control.
Body diameter is 1.12 inches.
A port for 1/16 inch temperature probe is provided.
Dual o-rings attach to the coverslip or other flat surface.
Patent Pending
C&L Instruments, Inc.
717-564-9491
Vacu-Cell
TM
System
Laminar Flow Round
The laminar flow incubation perfusion chamber forms a small laminar flow rectangular flow chamber using a coverslip as one surface. The chamber body has a diameter of 1.12 inches and is 0.65 inches tall. The coverslip is held tightly against the chamber body by a vacuum, forming a chamber 250 microns in depth. Other depths are available on request. You can use the Model VCS-1 vacuum pump system provided by C&L Instruments or an ordinary laboratory vacuum.
The Vacu-Cell forms a chamber with virtually any flat surface. You can even use the Vacu-Cell directly with a 6-well microtitre plate or Petri dish without the need for a coverslip.
The laminar flow design is available for 18 and 25 mm diameter coverslips. Both styles use the same microscope chamber holder. The Stainless Steel and Aluminum versions are also available with an integrated heating element and temperature probe port.
Model VC-LFR-25
Model Numbers
VC-LFR-25-SS VC-LFR-25-BD VC-LFR-25-CA VC-LFR-25-BA VC-LFR-25-SS-H VC-LFR-25-BA-H VC-LFR-18-CA VC-LFR-18-BA VC-LFR-18-BA-H VC-25RCS-1 VC-25RCS-2 VC-18RCS-1 VC-18RCS-2 VC-25-ORINGS VC-18-ORINGS
Stainless Steel, 25 mm Black Delrin, 25 mm Clear Acrylic, 25 mm Black Anodized Aluminum, 25 mm Stainless Steel, 25 mm, with Heater Black Anodized Aluminum, 25 mm, Heater Clear Acrylic, 18 mm Black Anodized Aluminum, 18 mm Black Anodized Aluminum, 18 mm, Heater 25 mm No. 1 Round Coverslips, Box of 160 25 mm No. 2 Round Coverslips, Box of 110 18 mm No. 1 Round Coverslips, Box of 310 18 mm No. 2 Round Coverslips, Box of 300 O-rings for 25 mm series, 10 Sets O-rings for 18 mm series, 10 Sets
Model VC-LFR-18
VC-LFR-25 in a 6 well plate
Model VC-LFR-18-H
C&L Instruments, Inc.
717-564-9491
Vacu-Cell
TM
System
Center Perfusion Round
The Center Perfusion incubation perfusion chamber forms a small round flow chamber using a coverslip or other flat surface. The chamber body has a diameter of 1.12 inches and is 0.65 inches tall. The coverslip is held tightly against the chamber body by a vacuum. You can use the Model VCS-1 vacuum pump system provided by C&L Instruments with this chamber, or an ordinary laboratory vacuum.
This perfusion chamber is designed for small volumes. With the center tube placed 0.5 mm above the coverslip, a 40 µl chamber is formed. It is ideal for TIRF microscopy or protocols where small volumes are preferred and access to the entire bottom surface of the coverslip is desired.
This chamber design is available for 18 and 25 mm diameter coverslips. Both styles can use the same microscope chamber holder. Aluminum versions are coated with Parylene for a biologically-compatible surface and are also available with an integrated heating element and temperature probe port.
Model VC-CPR-25
Model Numbers
VC-CPR-25-CA VC-CPR-25-BA VC-CPR-25-BA-H VC-CPR-18-CA VC-CPR-18-BA VC-CPR-18-BA-H VC-25RCS-1 VC-25RCS-2 VC-18RCS-1 VC-18RCS-2 VC-25-ORINGS VC-18-ORINGS
Vacuum
Port
Clear Acrylic, 25 mm Black Anodized Aluminum, 25 mm Black Anodized Aluminum, 25 mm, heater Clear Acrylic, 18 mm Black Anodized Aluminum, 18 mm Black Anodized Aluminum, 18 mm, Heater 25 mm No.1 Round Coverslips, Box of 160 25 mm No. 2 Round Coverslips, Box of 110 18 mm No. 1 Round Coverslips, Box of 310 18 mm No. 2 Round Coverslips, Box of 300 O-rings for 25 mm series, 10 Sets O-rings for 18 mm series, 10 Sets
Inlet/Outlet Ports
Coverslip
Model VC-CPR-25
(mid-section view)
C&L Instruments, Inc.
717-564-9491
Vacu-Cell
TM
System
Open Top Round
The Open Top incubation chamber forms a small round chamber using a coverslip or other flat surface. The chamber body has a outer diameter of 1.12 inches. A 25 mm diameter coverslip, or other flat surface, is held tightly against the chamber body by a vacuum. You can use a vacuum pump system provided by C&L Instruments or an ordinary laboratory vacuum.
Dual o-rings isolate the vacuum from the area of the coverslip under study and securely hold the coverslip in place. Chambers are formed quicky and easily, without the need for screws, nuts or retaining rings.
The Open Top configuration allows direct access to the top of the coverslip. The observation window measures 17.5 mm diameter. The thickness of the body measures 3 mm. The Open Top Round chamber is available in black anodized aluminum that is coated with parylene for biological compatibility.
Model Numbers
Model VC-OTR-25
(top view)
Model VC-OTR-25
(bottom view)
VC-OTR-25-BA VC-25RCS-1 VC-25RCS-2
VC-25M-ORINGS
Patent Pending
Black Anodized Aluminum for 25 mm coverslip 25 mm Round Coverslips, No. 1, Box of 160 25 mm Round Coverslips, No. 2, Box of 110
Replacement O-rings for OTR-25 chamber, 10 Sets
C&L Instruments, Inc.
717-564-9491
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