C&L introduces the Vacu-Cell line of cell incubation chambers. This unique
chamber design holds a coverslip against the chamber body by one side only,
providing major advantages over other types of cell chambers. Several
models are available to fit your specific application.
Advantages of the Vacu-Cell:
- Complete access to the bottom of the chamber, all the
way to the edges. Designed for cell and surface
microscopy, these chambers are perfect for oil immersion
objectives and TIRF experiments when access across the
entire coverslip is desired.
- A chamber is formed very simply and quickly, with no snaps,
screws or fixtures. Coverslips do not break!
- There are no parts to lose or break.
- Many configurations and styles are available, including
open top and laminar flow configurations.
- The chamber is formed directly from the coverslip, Petri
dish or microtitre plate by suction.
*Patent Pending
C&L Instruments, Inc.
www.fluorescence.com
717-564-9491
Anatomy of a Vacu-Cell
TM
- Round Series
The unique feature of all Vacu-Cell incubation chambers is the use of a vacuum generated
force to hold the optical surface tightly against to the body of the chamber. Dual o-rings are
used to separate the evacuated space from the remainder of the chamber. Several types of
chamber configurations are available, including chambers for circular and rectangular
coverslips. Chambers are formed quickly and easily without the need for screws, nuts or
retaining rings. This specification sheet describes the Round Series of chambers. Visit our
website for decriptions of other series.
Inlet and outlet tubes can be either 1/16
(standard) or 1/8 in. OD. Tubes are available in
steel or plastic, and in a variety of ID's.
The vacuum
port connects
to 1/16 in. ID tubing
Several flow patterns are
available. The flow
through this laminar
perfusion chamber is
shown by arrows.
Connectors are standard 1/4-28 in.
fittings, that can accept either 1/16 or
1/8 in. OD tubing. Tubing is held securely
with a ferrule. Hose barb connections for
1/16 in. ID tubing are available as an option.
Most chamber bodies are
available in several materials, including
aluminum, stainless steel, black delrin and
clear acrylic.
Most models are available
with an integrated heating
element for precise
temperature
control.
Body diameter is 1.12 inches.
A port for 1/16
inch temperature
probe is provided.
Dual o-rings attach to the
coverslip or other flat surface.
Patent Pending
C&L Instruments, Inc.
www.fluorescence.com
717-564-9491
Vacu-Cell
TM
System
Laminar Flow Round
The laminar flow incubation perfusion chamber forms a small laminar
flow rectangular flow chamber using a coverslip as one surface. The
chamber body has a diameter of 1.12 inches and is 0.65 inches tall.
The coverslip is held tightly against the chamber body by a vacuum,
forming a chamber 250 microns in depth. Other depths are available
on request. You can use the Model VCS-1 vacuum pump system
provided by C&L Instruments or an ordinary laboratory vacuum.
The Vacu-Cell forms a chamber with virtually any flat surface. You
can even use the Vacu-Cell directly with a 6-well microtitre plate or
Petri dish without the need for a coverslip.
The laminar flow design is available for 18 and 25 mm diameter
coverslips. Both styles use the same microscope chamber holder.
The Stainless Steel and Aluminum versions are also available with an
integrated heating element and temperature probe port.
Stainless Steel, 25 mm
Black Delrin, 25 mm
Clear Acrylic, 25 mm
Black Anodized Aluminum, 25 mm
Stainless Steel, 25 mm, with Heater
Black Anodized Aluminum, 25 mm, Heater
Clear Acrylic, 18 mm
Black Anodized Aluminum, 18 mm
Black Anodized Aluminum, 18 mm, Heater
25 mm No. 1 Round Coverslips, Box of 160
25 mm No. 2 Round Coverslips, Box of 110
18 mm No. 1 Round Coverslips, Box of 310
18 mm No. 2 Round Coverslips, Box of 300
O-rings for 25 mm series, 10 Sets
O-rings for 18 mm series, 10 Sets
Model VC-LFR-18
VC-LFR-25 in a
6 well plate
Model VC-LFR-18-H
C&L Instruments, Inc.
www.fluorescence.com
717-564-9491
Vacu-Cell
TM
System
Center Perfusion Round
The Center Perfusion incubation perfusion chamber forms a small
round flow chamber using a coverslip or other flat surface. The
chamber body has a diameter of 1.12 inches and is 0.65 inches
tall. The coverslip is held tightly against the chamber body by a
vacuum. You can use the Model VCS-1 vacuum pump system
provided by C&L Instruments with this chamber, or an ordinary
laboratory vacuum.
This perfusion chamber is designed for small volumes. With the
center tube placed 0.5 mm above the coverslip, a 40 µl chamber
is formed. It is ideal for TIRF microscopy or protocols where small
volumes are preferred and access to the entire bottom surface of
the coverslip is desired.
This chamber design is available for 18 and 25 mm diameter
coverslips. Both styles can use the same microscope chamber
holder. Aluminum versions are coated with Parylene for a
biologically-compatible surface and are also available with an
integrated heating element and temperature probe port.
Clear Acrylic, 25 mm
Black Anodized Aluminum, 25 mm
Black Anodized Aluminum, 25 mm, heater
Clear Acrylic, 18 mm
Black Anodized Aluminum, 18 mm
Black Anodized Aluminum, 18 mm, Heater
25 mm No.1 Round Coverslips, Box of 160
25 mm No. 2 Round Coverslips, Box of 110
18 mm No. 1 Round Coverslips, Box of 310
18 mm No. 2 Round Coverslips, Box of 300
O-rings for 25 mm series, 10 Sets
O-rings for 18 mm series, 10 Sets
Inlet/Outlet Ports
Coverslip
Model VC-CPR-25
(mid-section view)
C&L Instruments, Inc.
www.fluorescence.com
717-564-9491
Vacu-Cell
TM
System
Open Top Round
The Open Top incubation chamber forms a small
round chamber using a coverslip or other flat surface.
The chamber body has a outer diameter of 1.12 inches.
A 25 mm diameter coverslip, or other flat surface, is
held tightly against the chamber body by a vacuum.
You can use a vacuum pump system provided by C&L
Instruments or an ordinary laboratory vacuum.
Dual o-rings isolate the vacuum from the area of the
coverslip under study and securely hold the coverslip
in place. Chambers are formed quicky and easily,
without the need for screws, nuts or retaining rings.
The Open Top configuration allows direct access to
the top of the coverslip. The observation window
measures 17.5 mm diameter. The thickness of the body
measures 3 mm. The Open Top Round chamber is
available in black anodized aluminum that is coated
with parylene for biological compatibility.
Model Numbers
Model VC-OTR-25
(top view)
Model VC-OTR-25
(bottom view)
VC-OTR-25-BA
VC-25RCS-1
VC-25RCS-2
VC-25M-ORINGS
Patent Pending
Black Anodized Aluminum for 25 mm coverslip
25 mm Round Coverslips, No. 1, Box of 160
25 mm Round Coverslips, No. 2, Box of 110
Replacement O-rings for OTR-25 chamber, 10 Sets
C&L Instruments, Inc.
www.fluorescence.com
717-564-9491
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