C&L Instruments RPC-LW User Manual

Vacu-Cell

TM

System

Rectangular Perfusion Chamber, Large Window
Model RPC-LW

The Model RPC-LW perfusion chamber uses two rectangular coverslips to form a perfusion chamber.
Dual o-rings on the top of the chamber body and dual o-rings on the bottom securely hold two 48 x 65
mm coverslips in place. Each coverslip is held in place using a separate vacuum port, so they can be
used independently.

When the Model RPC-LW is used with two coverslips, a rectangular chamber is formed with the two
coverslips spaced by approximately 0.24 inches (6.1 mm). The total volume in the observation window is

7.54 ml. The observation window measures 1.2 x 1.6 inches (30.5 x 40.6 mm). Inlet and outlet perfusion
ports direct fluid along the bottom edge. The perfusion ports can be configured for use with 1/16 inch
OD tubing (shown), with 1/8 inch OD tubing, or with hose barb connectors. This chamber is available
in black anodized aluminum that is coated with parylene for biological compatibility. A port for a
temperature sensor is provided. Temperature is controlled using a heated microscope stage adapter.

Top and bottom

vacuum ports

Model VC-RPC-LW

Model Numbers

48 x 65 mm coverslip

Model VC-RPC-LW

(bottom view, without

bottom coverslip)

(top view)

Model VC-RPC-LW

(sectioned view)

VC-RPC-LW-BA
VC-48x65CS-1
VC-RPC-LW-ORINGS

Patent Pending

Large Window RPC, Black Anodized Aluminum
Rectangular 48 x 65 mm No. 1 Coverslips, 1 Box
Replacement O-rings for RPC-LW, 5 Sets

C&L Instruments, Inc.

www.fluorescence.com

717-564-9491