C&L Instruments RPC-1x3 User Manual

Vacu-Cell

TM

System

Rectangular Perfusion Chamber
Model RPC-1x3

The Model RPC-1x3 chamber is design to form a
laminar flow perfusion chamber using a standard
1 x 3 inch microscope slide. The chamber is formed
using the slide as one surface and the chamber body
as the other. The slide is held tightly against the chamber
body by a vacuum. You can use the Model VCS-1 vacuum
pump system provided by C&L Instruments or an ordinary laboratory
vacuum.

The dimensions of the laminar flow chamber are 12 mm (width) x 55 mm(length)
by 250 micron (depth). The total fluid volume in the laminar flow area is 165 µl. Other sizes are
available by special order. Perfusion port connectors are standard 1/4-28 inch fittings that can
accept either 1/16 or 1/8 inch OD tubing. This chamber is available in black anodized aluminum
that is coated with parylene for biological compatibility.

This chamber offers complete access to the bottom of the slide, all the way to the edges without
interference by any component of the chamber. This is perfect for oil immersion objectives and
TIRF experiments, when access across the entire slide is preferred. Holders are availabe to fit most
popular inverted microscope stages.

This flow chamber can be used with either standard or pre-coated microscope slides. C&L offers
several types of precoated slides that permit easy attachment of proteins, oligos or DNA to the slide
surface.

Model VC-RPC-1x3

bottom view without

attached slide

Part Numbers

VC-RPC-1x3-CA
VC-RPC-1x3-BA
VC-RPC-1x3-ORINGS

Model VC-RPC-1x3

sectioned view with

attached slide

Clear Acrylic
Black Anodized Aluminum
Replacement O-rings, 5 Sets

C&L Instruments, Inc.

www.fluorescence.com

717-564-9491