C&L Instruments MPC-LW User Manual

Vacu-Cell

TM

System

Mini Perfusion Chamber, Large Window
Model MPC-LW

The Mini series Large Window Perfusion Chamber uses two round 25 mm coverslips to form perfusion
chamber. Dual o-rings on the bottom and top of the chamber body securely hold the coverslips in
place. Each coverslip is held using a separate vacuum port, so they can be used independently. The
observation window measures 17.5 mm diameter.

When the Model MPC-LW is used with two coverslips, a circular chamber is formed with the two
coverslips spaced by 3.7 mm. The total volume in the observation window is approximately 890 µl. The
perfusion ports are configured for use with 1/16 OD tubing. The perfusion ports direct fluid flow from
one end to the other between the two coverslips.

The lower coverslip can be used without the upper coverslip to fashion an open chamber
configuration, allowing access to the coverslip surface. The top coverslip can be added at any time to
close the chamber for perfusion. Temperature is controlled using a heated microscope adapter. This
perfusion chamber works well using either an inverted or standard microscope. This chamber is
available in black anodized aluminum and is coated with parylene for biological compatibility.

Model VC-MPC-LW

(sectioned view)

Model Numbers

VC-MPC-TW-BA

VC-25RCS-1

VC-25RCS-2

VC-MPC-LW-ORINGS

Top and bottom

vacuum ports

Large Window MPC, Black Anodized
Aluminum
25 mm No. 1 Round Coverslips
Box of 160
25 mm No. 2 Round Coverslips
Box of 110
O-rings for MPC-LW, 5 Sets

The observation

window measures

17.5 mm diameter

Model VC-MPC-LW

(top view)

C&L Instruments, Inc.

Patent Pending

www.fluorescence.com

717-564-9491