Cleaver Scientific OmniPAGE Series, OmniPAGE Mini, omniPAGE Mini Wide, omniPAGE Maxi, omniPAGE VS30 Instruction Manual

Version 1

OmniPAGE Vertical Electrophoresis Systems
OmniPAGE Mini

Instruction Manual

Catalogue No: CVS10D

CVS10DSYS
CVS10PRE
CVS10DSYS-CU

Page 2

Table of Contents

Page

Section 1 Safety Information 3

1.1 Safety Precaution 3

Section 2 General Information 4

2.1 Introduction 4

2.2 Product Description 4

2.3 Packing Lists 5

2.4 Specifications 6

2.5 Care and Maintenance 7

Section 3 Operating Instructions 8

3.1 Setting up the omniPAGE Gel Tank 8

3.2 Gel casting 8

3.3 omniPAGE module assembly and Sample loading 13

3.4 Gel Running 14

3.5 Gel Removal 14

Section 4 Gel Preparation 14

4.1 Gel Selection 14

4.2 Volumes required per gel 15

4.3 Gel Preparation 16

4.4 Stacking Gel Preparation 16

4.5 Buffer Volume 17

4.6 Gel Running Conditions 17

4.7 Preparation of denatured protein samples for loading 18

4.8 Stock Solutions 19

Section 5 Trouble Shooting Guide 20

Section 6 Product Information 23

6.1 Catalogue numbers and product descriptions 23

6.2 Combs 24

6.3 Catalogue numbers and product descriptions for CSL Related products 26

Section 7 Warranty 27

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Section 1 Safety Information

1.1 Safety Precaution

When used correctly, these units pose no health risk. However, these units can deliver dangerous levels of

electricity and are to be operated only by qualified personnel following the guidelines laid out in this

instruction manual. Anyone intending to use this equipment should read the complete manual thoroughly.

The unit must never be used without the safety lid correctly in position. The unit should not be used if there is

any sign of damage to the external tank or lid.

Acrylamide is a powerful neurotoxin in solution form. Polymerized gels can contain some unpolymerized

solution and protective gloves and clothing must be worn.

These units comply with the following European directives:

2006/95/CE Low Voltage Directive and 2014/30/UE (official Title 2004/108/EC) EMC Electromagnetic
Compatibility

By virtue of the following harmonised standards:

BS EN IEC 61010-1: 2010 Safety Testing of Lab Equipment
BS EN IEC 61326-1:2013 EMC Electro Magnetic Compatibility

Page 4

Section 2 General Information

2.1 Introduction

Cleaver Scientifics’ omniPAGE range of Vertical Gel Units combines ease of use with high resolution
separations. Four sizes, Mini 10 x 10cm, Mini Wide 20 x 10cm and Maxi 20 x 20cm, VS 30 x 30cm share a host
of common features including a guaranteed leak proof seal required for trouble free, rapid and uncomplicated
gel casting. Utilising a built in gel running module eliminates time consuming transfer of glass plates during
casting, a process which can cause gel damage and misalignment. Glass plates with permanently bonded
spacers guarantee perfect spacer alignment. The glass plate sandwich is then simply inserted between
pressure bars and the new zero screw slide clamps clicked into position. This ensures fast set up times while
even pressure bars and ultra soft seals guarantee leak proof casting. Once the gel has polymerised, the gel
running module is just inserted into the gel tank for electrophoresis.

2.2 Product Description

Cleaver Scientifics’ vertical electrophoresis units comes as a complete package including Gel Tank, Safety Lid,
Gel Casting Inner Module, Glass plates with bonded spacers, Combs, Multipurpose Key, Power Cables and
Cooling pads. Detailed description of all the components is written below.

Gel Tank and Safety
Lid

Injection moulded gel tank and safety lid provides a sealed electrophoresis system
which is compatible with all major types of 8 X 10cm and 10 x 10cm precast gel. All
omniPAGE tanks are equipped with specially designed thumb locators for lifting up
the safety lid easily. All safety lids are designed to accommodate the polarity
design of the unit. They have special tapped holes which connect with the leading
edge of the power cables.

Inner Module

Injection moulded inner module gives a twofold effectiveness for gel casting and
running, thus no need of transferring the glass plates after casting the gel.

Glass Plates

2mm thick glass plates for the omniPAGE mini and 4mm thick glass plates for rest
of the range prevents breakage and have bonded spacers for convenience. All our
spacers are colour coded depending upon their thickness.

Multipurpose Key

CVS10KEY or the multipurpose key can be easily used to separate your notched
and plain glass plate to release the gel. The same key can be used to open the
CVS10 clamping doors.

Power Cables

Cleaver Scientifics’ power cables are designed with protective retractable

connectors which are compatible with most power supplies.

Cooling Pads

Rapid set up cooling packs enhance resolution eliminating the need of a chiller.

Combs with special
gel loading guides

Special combs (Combicombs) are designed with special oval shaped gel loading
guides on the other end of an ordinary comb which can be used.

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2.3 Packing Lists

CVS10D, CVS10DSYS, CVS10PRE, CVS10DSYS-CU

Each unit includes a tank, lid, internal module, electrodes and the following accessories:

Glass Plates

Combs

Casting base

Cooling Pack

Cables

Screws

CVS10D

VS10NG – Notched,

Pk/2

VS10PGS1 – Plain with

bonded 1mm spacers,

Pk/2

VS10-DP – Dummy

Plate

2 of VS10-12-1

1mm thick, 12

sample

VS10ICB

CSL-CAB

VS10-SCREW x 4

CVS10DSYS

VS10NG – Notched,

Pk/2

VS10PGS1 – Plain with

bonded 1mm spacers,

Pk/2

VS10-DP – Dummy

Plate

2 of VS10-12-1

1mm thick, 12

sample

VS10DCAST

VS10DCASTM –

Mat

VS10ICB

CSL-CAB

VS10-SCREW x 4

CVS10PRE

VS10-DP – Dummy

Plate

VS10ICB

CSL-CAB

VS10-SCREW x 4

CVS10DYS-CU

See CVS10DYSYS

CVS10EXCASTER

See

CVS10DYSYS

See CVS10DYSYS

VS10ICB

CSL-CAB

VS10-SCREW x 4

Packing list checked by ________________________ Date ____________

The packing lists should be referred to as soon as the units are received to ensure that all components have

been included. The unit should be checked for damage when received.

Please contact your supplier if there are any problems or missing items.

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2.4 Specifications of OmniPAGE Vertical Electrophoresis Units

omniPAGE Mini

omniPAGE Mini

Wide

omniPAGE Maxi

omniPAGE VS30

Plate
Dimensions
Gel Dimensions
(WxL)

10x10cm

7.5x8cm

20 X 10cm
18 X 8cm

20 X 20CM
16 X 17.5CM

30 X 22cm
28 X 20cm

Unit Dimensions

19x13x15cm
(W x D x H)

26 X 16 X 16cm
(W X D X H)

26 X 16 X 28CM
(W X D X H)

36 X 33 X 18CM
(W X H X D)

Max Sample
Capacity

80 Samples
20 Samples per Gel

192 Samples
48 Samples per Gel

192 Samples
48 Samples per Gel

300 Samples per
Run
75 Samples per Gel

Buffer Volume

Min 250ml,
Max 1200ml

Min 600ml
Max 2800ml

Min 1200ml
Max 5600ml

Min1800ml
Max 8400ml

Combs Available

No. of Teeth
Thickness

1, 5, 8MC, 9, 10,
12, 16MC, 20

0.75, 1, 1.5, 2mm

1, 5, 10, 18MC, 24,
30, 36MC, 48

0.75, 1, 1.5, 2mm

1, 5, 10, 18MC, 24,
30, 36MC, 48

0.75, 1, 1.5, 2mm

1, 2, 4, 28MC,
56MC, 75

0.25, 0.35, 0.5, 1,

1.5, 2.0mm

Environmental
Operating
Conditions

Maximum Altitude
2,000 m
Temperature
Range 4°C - 65°C
Humidity
Upto 80%
Not for outdoor
Use

Maximum Altitude
2,000 m
Temperature
Range 4°C - 65°C
Humidity
Upto 80%
Not for outdoor
Use

Maximum Altitude
2,000 m
Temperature
Range 4°C - 65°C
Humidity
Upto 80%
Not for outdoor
Use

Maximum Altitude
2,000 m
Temperature
Range 4°C - 65°C
Humidity
Upto 80%
Not for outdoor
Use

This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664.

POLLUTION DEGREE 2, states that: “Normally only non-conductive pollution occurs.

Occasionally, however, a temporary conductivity caused by condensation must be expected”.

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2.5 Care and Maintenance

Cleaning Large Format Vertical Units
Units are best cleaned using warm water and a mild detergent. Water at temperatures above 600 C can cause
damage to the unit and components.

The inner module should be thoroughly rinsed with warm water or distilled water to prevent buildup of salts
but care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or
advised. Air drying preferably before use.

The units should only be cleaned with the following:

Warm water with a mild concentration of soap or other mild detergent.
Compatible detergents include dishwashing liquid, Hexane and Aliphatic hydrocarbons. The units should not
be left in detergents for more than 30 minutes.

The units should never come into contact with the following cleaning agents, these will cause irreversible
and accumulative damage:

Acetone, Phenol, Chloroform, Carbon tetrachloride, Methanol, Ethanol, Isopropyl alcohol, Alkalis.

RNase Decontamination

This can be performed using the following protocol:­Clean the units with a mild detergent as described above.
Wash with 3% hydrogen peroxide (H2O2) for 10 minutes.
Rinsed with 0.1% DEPC- (diethyl pyrocarbonate) treated distilled water.

Caution: DEPC is a suspected carcinogen. Always take the necessary precautions when using. RNaseZAP™

(Ambion) can also be used. Please consult the instructions for use with acrylic gel tanks.

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Section 3 Operating Instructions

3.1 Setting up the omniPAGE Gel Tank

Note: Before setting up the Gel Tank please ensure that it has been properly cleaned and dried.

1. Note the position of the lid on the unit. This shows the correct polarity and the correct orientation of the

cables, black is negative and red positive.

2. Remove the lid from the unit.

Note: If the lid is not removed, fitting the cables may result in un-tightening of the gold plug and damage

the electrode.

3. Screw the cables into the tapped holes as fully as possible so that there is no gap between the lid and the

leading edge of the cable fitting.

4. Refit the lid and the unit is now ready to be used.

3.2 Gel casting

Cleaning the Glass Plates

Clean a set of glass plates for each gel first with distilled water and then with 70 % ethanol.

One set of glass plates constitutes one notched glass plate and one plain glass plate with bonded spacers.

When using a triple glass plate sandwich, two notched glass plates are required, one set of free spacers and a

set of plain glass plates with bonded spacers. The plain glass plate is positioned outermost, then a notched

glass plate, free spacers and second notched glass plate. Alternatively, accessory notch glass plates with

bonded spacers are available.

Note: All glass plates, gel casting modules, casting base and accessories must be completely dry before the
set – up. Wet components are more likely to miss-align and cause leaks.

Page 9

Glass cassette Assembly

Assemble the glass plates so that the bottom of the glass plates and the spacers are perfectly aligned.

For triple plate sandwiches, the free spacers Need to be perfectly aligned which is best performed using a

small spacer or comb to push the spacers apart. Notched glass plates with bonded spacers do not need

manual alignment.

NOTE: The glass plates with bonded spacers have an arrow in the top of the spacers which are slightly
longer than the glass plate to indicate the top.

Casting Stand Assembly

Position the Slab Gel Insert on a flat surface.

Insert the glass plates into the Slab Gel Insert

between the pressure bar and the blue gasket.

The Slab Gel Insert contains pressure bars

which impart even pressure onto the glass plates

and allow even screw pressure transfer onto the

sealing edge of the glass plate, ensuring complete sealing. Ensure that the pressure bars are adequately open

for the thickness of spacer used. The bar can be opened by loosening the screws or by sliding the clamps.

When using a triple glass plate sandwich, the pressure bars will need to be in the completely open position.

Then fully tighten the pressure bar screws in the order top then bottom.