Cleaver Scientific MSMIDI10, MSMIDI7, MSMIDIDUO, MSCHOICE7, MSCHOICE10 Instruction Manual

multiSUB Horizontal System

®

multiSUB

Horizontal

Electrophoresis Units

Instruction Manual

Catalogue Numbers

MSMINI7

MSMINI10

MSMINIDUO

MSCHOICE7

MSCHOICE10

MSCHOICE15

MSCHOICETRIO

MSCHOICEST20

MSCHOICEST25

MSMIDI7

MSMIDI10

MSMIDIDUO

MSMAXI10

MSMAXI15

MSMAXI20

MSMAXIDUO

MSMAXI25

Record the following for your records:

Model _____________________

Catalogue No. _____________________

Date of Delivery _____________________

Warranty Period _____________________

Serial No. _____________________

Invoice No. _____________________

Purchase Order No. _____________________

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Contents

Instruction Manual 1

Catalogue Numbers 1

Contents 2

Safety Information 4

Packing List 5

Specifications 7

Operating Instructions 8

Usage Guidance and restrictions 8

Setting up the Horizontal Gel Tanks 8

Fitting Electrodes 8

Fitting Loading Guides 8

Gel Preparation 9

Gel Pouring 10

Casting Dams 10

Flexicaster 12

Tape 13

Running the Gel 13

Gel Staining and Viewing 14

Solutions 14

References 15

Troubleshooting 16

Care and Maintenance 18

Cleaning Horizontal Units 18

RNAse Decontamination 18

Ordering information 19

Comb options 21

Related Products 26

Warranty 28

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Safety Information

When used correctly, these units pose no health risk. However, these units can deliver dangerous levels of electricity and are to be operated only by qualified personnel following the guidelines laid out in this instruction manual. Anyone intending to use this equipment should read the complete manual thoroughly. The unit must never be used without the safety lid correctly in position. The unit should not be used if there is any sign of damage to the external tank or lid.

These units comply with the following European directives:

2006/95/CE Low Voltage Directive and 2014/30/UE (official Title 2004/108/EC) EMC Electromagnetic Compatibility

By virtue of the following harmonised standards:

BS EN IEC 61010-1: 2010 Safety Testing of Lab Equipment

BS EN IEC 61326-1:2013 EMC Electro Magnetic Compatibility

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Packing List

Each multiSUB unit includes a tank, wired electrodes, lid and the following items:

SKU Tray Tray Dams Combs Loading Guides Cables

MSMINI7 MS7-UV7, 7 x

7cm (W x L)

MSMINI10 MS7-UV10, 7

x 10cm (W x L)

MSMINIDUO MS7-UV7,

MS7-UV10

MSMIDI7 MS10-UV7, 10

x 7cm (W x L)

MSMIDI10 MS10-UV10,

10 x 10cm (W x L)

MSMIDIDUO MS10-UV7,

MS10-UV10

MS7-UVDAM, Pack of 2

MS10-UVDAM, Pack of 2

2 x MS7-8-1, 1mm, 8 sample

2 x MS10-16-1, 1mm, 16 sample

MS7-LG – Strips, MS7-WP – Platform

MS10-LG – Strips, MS10-WP – Platform

CSLCAB

MSCHOICE7 MS15-UV7, 15

x 7cm (W x L)

MSCHOICE10 MS15-UV10,

15 x 10cm (W x L)

MSCHOICE15 MS15-UV15,

15 x 15cm (W x L)

MSCHOICETRIO MS15-UV7,

MS15-UV10, MS15-UV15

MSCHOICEST20 MS15-UVST20

15 x 20 cm, (W x L)

MSCHOICEST25 MS15-UVST25

15 x 25 cm, (W x L)

MS15-UVDAM, Pack of 2

2 x MS15-20-1, 1mm, 20 sample

4 x MS15-28MC1, 1mm thick, 28 sample MC

MS15-LG – Strips, MS15-WP – Platform

CSLCAB

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MSMAXI10 MS20-UV10,

20 x 10cm (W x L)

MS20-UVDAM, Pack of 2

2 x MS20-20-1, 1mm, 20 sample

MS20-LG – Strips, MS20-WP – Platform

CSLCAB

MSMAX15 MS20-UV15,

20 x 15cm (W x L)

MSMAXI20 MS20-UV20,

20 x 20cm (W x L)

MSMAXIDUO MS20-UV10,

MS20-UV20

MSMAXI25 MS20-UV25,

20 x 25cm (W x L)

MS20-UVDAM, Pack of 2

2 x MS20-20-1, 1mm, 20 sample

Packing List Checked by: ________________________

Date: ________________________

MS20-LG – Strips, MS20-WP – Platform

CSLCAB

The packing lists should be referred to as soon as the units are received to ensure that all components have been included. The unit should be checked for damage when received.

Cleaver Scientific is liable for all missing or damaged parts / accessories within 7 days after customers have received this instrument package. Please contact Cleaver Scientific immediately regarding this issue. If no response within such period is received from the customer, Cleaver Scientific will no longer be liable for replacement/damaged parts.

Please contact your supplier if there are any problems or missing items.

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Specifications

1 Power supply cables 1500V rated with retractable 4mm connectors

2 Safety lid and viewing pane High impact moulded acrylic construction

3 Height adjustable combs Acrylic

4 UV transparent gel tray UV transparent above 300nm, Moulded acrylic

5 Comb Slots Allow multiple combs per tray

6 Casting dams Natural rubber

7 Colour-coded electrodes Moulded acrylic, 99.99% platinum wire, gold plated

4mm plugs

8 Gel platform Notched to fix gel position

9 Safety lid thumb locators For safe removal of lid

10 Moulded buffer tank High Impact moulded acrylic construction for chemical

compatibility and shock resistance

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Operating Instructions

Further information (including videos) regarding setting up and running the multiSUB® units can be found at www.cleaverscientific.com

Usage Guidance and restrictions

 Maximum altitude 2,000m.

 Temperature range between 4°C and 65°C.

 Maximum relative humidity 80% for temperatures up to 31OC

decreasing linearly to 50% relative humidity at 40OC.

 Not for outdoor Use.

This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664.

POLLUTION DEGREE 2, states that: “Normally only non-conductive pollution occurs.

Occasionally, however, a temporary conductivity caused by condensation must be expected”.

Setting up the Horizontal Gel Tanks

Fitting Electrodes

 Note the position of the lid on the unit. This shows the correct polarity

and the correct orientation of the cables, black is negative and red positive.

 Remove the lid from the unit. Note if the lid is not removed, fitting the

cables may result in un-tightening of the gold plug and damage to the electrode.

 Screw the cables into the tapped holes as fully as possible so that there

is no gap between the lid and the leading edge of the cable fitting.

 Refit the lid.

Fitting Loading Guides

These can be fitted to enhance visibility of the wells if desired. They can be fitted to the white vinyl platform sheet or to the unit itself.

1. Seat the tray in the unit and note the position of the comb grooves. The samples run black to red but the trays can be used frontward or backwards so ensure that the comb grooves closest to the black electrode are marked.

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Cleaver Scientific MSMIDI10, MSMIDI7, MSMIDIDUO, MSCHOICE7, MSCHOICE10 Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual