Cleaver Scientific MSMIDI10, MSMIDI7, MSMIDIDUO, MSCHOICE7, MSCHOICE10 Instruction Manual

multiSUB Horizontal System

®

multiSUB

Horizontal

Electrophoresis Units

Instruction Manual

Catalogue Numbers

MSMINI7

MSMINI10

MSMINIDUO

MSCHOICE7

MSCHOICE10

MSCHOICE15

MSCHOICETRIO

MSCHOICEST20

MSCHOICEST25

MSMIDI7

MSMIDI10

MSMIDIDUO

MSMAXI10

MSMAXI15

MSMAXI20

MSMAXIDUO

MSMAXI25

Record the following for your records:

Model _____________________

Catalogue No. _____________________

Date of Delivery _____________________

Warranty Period _____________________

Serial No. _____________________

Invoice No. _____________________

Purchase Order No. _____________________

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Contents

Instruction Manual 1

Catalogue Numbers 1

Contents 2

Safety Information 4

Packing List 5

Specifications 7

Operating Instructions 8

Usage Guidance and restrictions 8

Setting up the Horizontal Gel Tanks 8

Fitting Electrodes 8

Fitting Loading Guides 8

Gel Preparation 9

Gel Pouring 10

Casting Dams 10

Flexicaster 12

Tape 13

Running the Gel 13

Gel Staining and Viewing 14

Solutions 14

References 15

Troubleshooting 16

Care and Maintenance 18

Cleaning Horizontal Units 18

RNAse Decontamination 18

Ordering information 19

Comb options 21

Related Products 26

Warranty 28

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Safety Information

When used correctly, these units pose no health risk. However, these units can deliver dangerous levels of electricity and are to be operated only by qualified personnel following the guidelines laid out in this instruction manual. Anyone intending to use this equipment should read the complete manual thoroughly. The unit must never be used without the safety lid correctly in position. The unit should not be used if there is any sign of damage to the external tank or lid.

These units comply with the following European directives:

2006/95/CE Low Voltage Directive and 2014/30/UE (official Title 2004/108/EC) EMC Electromagnetic Compatibility

By virtue of the following harmonised standards:

BS EN IEC 61010-1: 2010 Safety Testing of Lab Equipment

BS EN IEC 61326-1:2013 EMC Electro Magnetic Compatibility

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Packing List

Each multiSUB unit includes a tank, wired electrodes, lid and the following items:

SKU Tray Tray Dams Combs Loading Guides Cables

MSMINI7 MS7-UV7, 7 x

7cm (W x L)

MSMINI10 MS7-UV10, 7

x 10cm (W x L)

MSMINIDUO MS7-UV7,

MS7-UV10

MSMIDI7 MS10-UV7, 10

x 7cm (W x L)

MSMIDI10 MS10-UV10,

10 x 10cm (W x L)

MSMIDIDUO MS10-UV7,

MS10-UV10

MS7-UVDAM, Pack of 2

MS10-UVDAM, Pack of 2

2 x MS7-8-1, 1mm, 8 sample

2 x MS10-16-1, 1mm, 16 sample

MS7-LG – Strips, MS7-WP – Platform

MS10-LG – Strips, MS10-WP – Platform

CSLCAB

MSCHOICE7 MS15-UV7, 15

x 7cm (W x L)

MSCHOICE10 MS15-UV10,

15 x 10cm (W x L)

MSCHOICE15 MS15-UV15,

15 x 15cm (W x L)

MSCHOICETRIO MS15-UV7,

MS15-UV10, MS15-UV15

MSCHOICEST20 MS15-UVST20

15 x 20 cm, (W x L)

MSCHOICEST25 MS15-UVST25

15 x 25 cm, (W x L)

MS15-UVDAM, Pack of 2

2 x MS15-20-1, 1mm, 20 sample

4 x MS15-28MC1, 1mm thick, 28 sample MC

MS15-LG – Strips, MS15-WP – Platform

CSLCAB

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MSMAXI10 MS20-UV10,

20 x 10cm (W x L)

MS20-UVDAM, Pack of 2

2 x MS20-20-1, 1mm, 20 sample

MS20-LG – Strips, MS20-WP – Platform

CSLCAB

MSMAX15 MS20-UV15,

20 x 15cm (W x L)

MSMAXI20 MS20-UV20,

20 x 20cm (W x L)

MSMAXIDUO MS20-UV10,

MS20-UV20

MSMAXI25 MS20-UV25,

20 x 25cm (W x L)

MS20-UVDAM, Pack of 2

2 x MS20-20-1, 1mm, 20 sample

Packing List Checked by: ________________________

Date: ________________________

MS20-LG – Strips, MS20-WP – Platform

CSLCAB

The packing lists should be referred to as soon as the units are received to ensure that all components have been included. The unit should be checked for damage when received.

Cleaver Scientific is liable for all missing or damaged parts / accessories within 7 days after customers have received this instrument package. Please contact Cleaver Scientific immediately regarding this issue. If no response within such period is received from the customer, Cleaver Scientific will no longer be liable for replacement/damaged parts.

Please contact your supplier if there are any problems or missing items.

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Specifications

1 Power supply cables 1500V rated with retractable 4mm connectors

2 Safety lid and viewing pane High impact moulded acrylic construction

3 Height adjustable combs Acrylic

4 UV transparent gel tray UV transparent above 300nm, Moulded acrylic

5 Comb Slots Allow multiple combs per tray

6 Casting dams Natural rubber

7 Colour-coded electrodes Moulded acrylic, 99.99% platinum wire, gold plated

4mm plugs

8 Gel platform Notched to fix gel position

9 Safety lid thumb locators For safe removal of lid

10 Moulded buffer tank High Impact moulded acrylic construction for chemical

compatibility and shock resistance

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Operating Instructions

Further information (including videos) regarding setting up and running the multiSUB® units can be found at www.cleaverscientific.com

Usage Guidance and restrictions

 Maximum altitude 2,000m.

 Temperature range between 4°C and 65°C.

 Maximum relative humidity 80% for temperatures up to 31OC

decreasing linearly to 50% relative humidity at 40OC.

 Not for outdoor Use.

This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664.

POLLUTION DEGREE 2, states that: “Normally only non-conductive pollution occurs.

Occasionally, however, a temporary conductivity caused by condensation must be expected”.

Setting up the Horizontal Gel Tanks

Fitting Electrodes

 Note the position of the lid on the unit. This shows the correct polarity

and the correct orientation of the cables, black is negative and red positive.

 Remove the lid from the unit. Note if the lid is not removed, fitting the

cables may result in un-tightening of the gold plug and damage to the electrode.

 Screw the cables into the tapped holes as fully as possible so that there

is no gap between the lid and the leading edge of the cable fitting.

 Refit the lid.

Fitting Loading Guides

These can be fitted to enhance visibility of the wells if desired. They can be fitted to the white vinyl platform sheet or to the unit itself.

1. Seat the tray in the unit and note the position of the comb grooves. The samples run black to red but the trays can be used frontward or backwards so ensure that the comb grooves closest to the black electrode are marked.

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2. Remove the tray.

3. Peel the back off the loading guide and carefully apply the loading guide directly to the gel platform.

The unit is now ready to be used.

Gel Preparation

The Table below shows the volume of agarose solution required to make the desired agarose gel for each unit tray size. For a standard 0.7% agarose gel, add 0.7 grams of agarose to 100 ml of 1x TAE or TBE solution. The same 1X solution should be used in the tank buffer solution.

multiSUB® Mini

multiSUB® Midi

multiSUB®

Choice

multiSUB®

Choice STRETCH

multiSUB® Maxi

Tray

Gel volume for a 5mm thick

gel

Tray

Gel volume for a 5mm thick

gel

Tray

Gel volume for a 5mm thick

gel

Tray

Gel volume for a 5mm thick

gel

Tray

7 x 7 cm 7 x 10 cm

25 mL 35 mL

10 x 7 cm 10 x 10 cm

35 mL 50 mL

15 x 7 cm 15 x 10 cm 15 x 15 cm

52.5 mL 75 mL 112.5 mL

15 x 20 cm 15 x 25 cm

150 mL 187.5 mL

20 x 10 cm 20 x 15 cm 20 x 20 cm

Gel volume for a 5mm thick

gel

100 mL 150 mL 200 mL

1. Add the agarose powder to a conical flask.

2. Add the appropriate amount of 1x TAE or TBE solution from the table above. To prevent evaporation during the dissolving steps below, the conical flask should be covered with parafilm.

3. Dissolve the agarose powder by heating the agarose either on a magnetic hot plate with stirring bar or in a microwave oven. If using the microwave method, the microwave should be set at around a 400 watt or medium setting and the flask swirled every minute. The solution

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should be heated until all crystals are dissolved. This is best viewed against a light background. Crystals appear as translucent crystals. These will interfere with sample migration if not completely dissolved.

The gel must be cooled to between 50°C and 60°C degrees before pouring.

Gel Pouring

The multiSUB® range of units allows three different methods of gel casting:

1. Casting Dams

2. Flexicaster

3. Traditional Tape

Casting Dams

1. To fit the casting dams, place one casting dam on the bench with the groove facing upwards (1). Push the edge of the tray down firmly into the groove (2). Repeat this for the other side (3). The dams should be fitted so that there is no gap between the sides of the tray and the groove in the dams. This will ensure that there is no possibility of gel leakage.

2. Place the comb(s) in the grooves. Each tray has more than one comb grove so that multiple combs can be used. Using multiple combs increases sample number available per gel but decreases run length and care must be taken to ensure that samples from the first wells do not migrate into the lanes of the second comb wells.

3. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that do occur can be smoothed to the edge of the gel and dispersed using a pipette tip.

4. Allow the agarose to set, ensuring that the gel remains undisturbed.

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5. Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank.

(1) (2) (3)

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Flexicaster

1. Level the Flexicaster base by adjusting the feet so that the bubble is exactly central.

2. Insert the desired length tray into the Flexicaster such that one end of the tray is pushed up and seals against the silicone mat of the permanent end of the Flexicaster.

3. Position the movable end of the Flexicaster so that the silicone mat is pushed against the other end of the tray.

4. Turn the cam so that the silicone mat tightly seals against the side of the tray. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that do occur can be smoothed to the edge of the gel and dispersed using a pipette tip.

5. Allow the agarose to set, ensuring that the gel remains undisturbed.

6. Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank.

MS7-FC and MS20-FC Flexicasters

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Tape

1. Autoclave or plastic backed general tape should be used. A length 5cm longer than the width of each end of the tray should be cut. One length should be placed over one end of the tray and stuck m1cm in from the tray edge. This should then be folded, and the edges sealed securely. Repeat for the other end and place onto a level surface for gel pouring.

2. Place the comb(s) in the grooves. Each tray has more than one comb grove so that multiple combs can be used. Using multiple combs increases sample number available per gel but decreases run length and care must be taken to ensure that samples from the first wells do not migrate into the lanes of the second comb wells.

3. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that do occur can be smoothed to the edge of the gel and dispersed using a pipette tip.

4. Allow the agarose to set, ensuring that the gel remains undisturbed.

5. Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank.

Running the Gel

1. Mix the sample to be loaded with sample buffer – see solutions for common sample buffers. Usually 3ul of sample buffer is adequate but less may be used with sample volumes of less than 10ul.

2. Fill the unit with buffer until the gel is just flooded with buffer. This will give the fastest resolution times. For enhanced quality of resolution of sample, fill the unit to 5mm above the gel.

3. Load the samples into the wells using pipettes. Multi-channel pipettes can be used for loading samples with MC compatible combs, see listing in accessories for identification of these.

4. Carefully place the lid on the tank and connect to a power supply.

5. Typically, gels are run at between 90 and 150 volts. However, maximum voltages are indicated on the serial badge of each unit. It should be noted that higher voltages generally give faster but poorer quality sample resolution.

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Gel Staining and Viewing

The Multi Sub trays allow staining to be performed without removing the gel from the tray if this is preferred.

1. Transfer the gel to a vessel containing the appropriate volume of 0.5 µg/ml ethidium bromide stain for 15–30 minutes, see solutions for stock stain concentration and adjust to the volume used accordingly. The entire gel should be covered.

NOTE: Ethidium bromide is a suspected carcinogen and the necessary safety precautions should be taken.

2. De-stain the gel for 10–30 minutes in distilled water again ensuring the gel is completely immersed.

3. Rinse the gel twice for a couple of seconds with distilled water.

4. Transfer the gel to a UV Transilluminator.

The samples will often appear as brighter, clearer bands when photographed or viewed using a gel documentation system. However, if the gel bands are too faint then the staining procedure should be adjusted so that there is less de-staining. If there is too much background, then the staining procedure should be adjusted so that there is more de-staining.

Solutions

0.5M EDTA stock (500mL) dissolve in 400 ml distilled water:

 93.05g EDTA disodium salt

Fill to 500 ml litre final volume with distilled water

50X TAE stock (1L) dissolve in 750 ml distilled water:

 242 g tris base (FW = 121)

 57.1 ml glacial acetic acid

 100 ml 0.5 M EDTA (pH 8.0).

Fill to 1 litre final volume with distilled water

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10X TBE stock (1L) dissolve in 750 ml distilled water:

 108 g tris base (FW = 121)

 55 g boric acid (FW = 61.8)

 40 ml 0.5 M EDTA (pH 8.0)

Fill to 1 litre final volume with distilled water

Loading Dye

10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol blue, and 0.25% xylene cyanole FF in 1x TAE buffer. Only 1–10 ml of the 10x loading dye should be prepared.

Ethidium Bromide Solution

Add 10 mg of Ethidium Bromide to 1 ml distilled water.

References

1. Sambrook, Fritsch, and Maniatis, Molecular Cloning A Laboratory

Manual, Second Edition, Cold Spring Harbor Laboratory Press, 1989.

2. Current Protocols in Molecular Biology, Greene Publishing Associates

and Wiley-Interscience, 1989.

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Troubleshooting

Problem Cause Solution

Bands sharp but not enough bands seen

Band smearing and streaking

Curved line or distortion of bands Bubbles in sample wells

Curved bands, smiles Sample overload Reduce load.

Gel agarose percentage too high

Incomplete digestion

Agarose has improper endosmosis

Salt concentration in sample too high

Excessive power and heating

Sample spilled out of well

Incomplete digestion, nuclease contamination, bad enzyme

Sample wells cast through the gel. Sample leaks along bottom of running surface

Decrease agarose percentage.

Review enzyme activity, digest further.

Reduce salt concentration to

≤0.1M.

Reduce voltage. See electrophoresis instructions.

Apply sample carefully. Increase gel thickness for large sample volumes. Adjust comb height.

Heat sample. Review enzyme activity. Digest sample further.

Comb should be placed to 1 to 2 mm above the base of the running surface.

Remove bubbles prior to electrophoresis.

Differential relative mobilities

Gels crack

High MW bands sharp; low MW bands smeared

Ragged bands

Sample spilled out of wells

Unit not levelled

Too high voltage gradient, especially with low melting temperature agarose or low gel strength gels

Gel agarose percentage too low

Sample density incorrect

Sample well deformed

Excessive power or heating

Samples should have proper density. Apply carefully.

Level unit. Use a steady work bench.

Reduce voltage. Run gel at lower temperature.

Increase agarose percentage. Switch to polyacrylamide.

See sample application instructions.

Carefully remove comb, especially from soft gels. Make sure gel has solidified.

Cooling soft gels aids in comb removal.

Reduce voltage. See electrophoresis instructions.

Slanted lanes (bands)

Gel not fully solidified

Comb warped or at an angle

Gel to solidify for at least 3045min.

Check alignment of comb.

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Care and Maintenance

Cleaning Horizontal Units

Units are best cleaned using warm water and a mild detergent. Water at temperatures above 60°C can cause damage to the unit and components.

The tank should be thoroughly rinsed with warm water or distilled water to prevent build-up of salts, but care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or advised.

Air drying is preferably before use.

The units should only be cleaned with the following:

Warm water with a mild concentration of soap or other mild detergent.

Compatible detergents include dishwashing liquid, Hexane and Aliphatic hydrocarbons

The units should not be left to in detergents for more than 30 minutes.

The units should never come into contact with the following cleaning agents, these will cause irreversible and accumulative damage:

Acetone, Phenol, Chloroform, Carbon tetrachloride, Methanol, Ethanol, Isopropyl alcohol, Alkalis.

RNAse Decontamination

This can be performed using the following protocol:

Clean the units with a mild detergent as described above.

Wash with 3% hydrogen peroxide (H2O2) for 10 minutes.

Rinsed with 0.1% DEPC-(diethyl pyro carbonate) treated distilled water,

Caution: DEPC is a suspected carcinogen. Always take the necessary precautions when using.

RNaseZAP™ (Ambion) can also be used. Please consult the instructions for use with acrylic gel tanks.

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Ordering information

Catalogue No.

MSMINI7 multiSUB® Mini, 7 x 7cm UV Tray, 2 x 8 sample combs, loading guides and dams

MSMINI10 multiSUB® Mini, 7 x 10cm UV Tray, 2 x 8 sample combs, loading guides and

MSMINIDUO multiSUB® Mini, 7 x 7cm & 7 x 10cm UV Tray, 2 x 8 sample combs, loading

MS7-UV7 7 x 7cm UV Tray

MS7-UV10 7 x 10cm UV Tray

MS7-PE Positive Electrode

MS7-NE Negative Electrode

MS7-UVDAM Casting Dams

MS7/10-FC multiSUB® Mini/Midi Flexi caster

MS7-LG Adhesive Loading Guides

MS7-WP Viewing Platform

Product description

dams

guides and dams

MSMINICP Cool-pack and Platform

MSMINIBSB Buffer Saver Blocks, pk/2 saves 100ml of buffer

MS7-UVS 7cm UV Gel Scoop

CSL-CAB Electrophoresis cable (Black & Red)

MSMIDI7 multiSUB® Midi, 10 x 7cm UV Tray, 2 x 16 sample combs, loading guides and

dams

MSMIDI10 multiSUB® Midi, 10 x 10cm UV Tray, 2 x 16 sample combs, loading guides and

dams

MSMIDIDUO multiSUB® Midi, 10 x 7cm & 10 x 10cm UV Tray, 2 x 16 sample combs, loading

guides and dams

MS10-UV7 10 x 7cm UV Tray

MS10-UV10 10 x 10cm UV Tray

MS10-PE Positive Electrode

MS10-NE Negative Electrode

MS10-UVDAM Casting Dams

MS7/10-FC multiSUB® Mini/Midi Flexi caster

MS10-LG Adhesive Loading Guides

MS10-WP Viewing Platform

MSMIDICP Cool-pack and Platform

MSMIDIBSB Buffer Saver Blocks, pk/2 saves 100ml of buffer

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MS10-UVS 10cm UV Gel Scoop

CSL-CAB Electrophoresis cable (Black & Red)

MSCHOICE7 multiSUB® ™ Choice 15 x 7cm UV Tray, 2 x 20 sample combs, loading guides

and dams

MSCHOICE10 multiSUB® ™ Choice 15 x 10cm UV Tray, 2 x 20 sample combs, loading guides

and dams

MSCHOICE15 multiSUB® ™ Choice 15 x 15cm UV Tray, 2 x 20 sample combs, loading guides

and dams

MSCHOICETRIO multiSUB® ™ Choice Trio 15 x 7,10 & 15cm UV Tray, 2 x 20 sample combs,

loading guides and dams

MSCHOICETRI O15

multiSUB® ™ Choice Trio 15 3 x 15cm UV tray, 2x 20 sample combs, loading guides and dams

MSCHOICEST20 multiSUB® ™ Choice Stretch 20 15 x 20cm UV tray, 4 x 28 sample combs,

loading guides and dams

MSCHOICEST25 multiSUB® ™ Choice Stretch 25 15 x 25cm UV tray, 4 x 28 sample combs,

loading guides and dams

MS15-UV7 15 x 7cm UV Tray

MS15-UV10 15 x 10cm UV Tray

MS15-UV15 15 x 15cm UV Tray

MS15-UVST20 15 x 20cm UV Tray

MS15-UVS 15cm UV Gel Scoop

MS15-UVDAM Casting Dams

MS15-PE Positive Electrode

MS15-NE Negative Electrode

MSMS15-LG Adhesive Loading Guides

MS15-WP Viewing Platform

MSCHOICECP Cool-Pack and Platform

MS15/20-FC multiSUB® Choice/ Maxi Flexi caster

MSMAXI10 20 x 10cm UV Tray, 2 x 20 sample, 1mm thick combs, casting dams, loading

guides

MSMAXI15 20 x 15cm UV Tray, 2 x 20 sample, 1mm thick combs, casting dams, loading

guides

MSMAXI20 20 x 20cm UV Tray, 2 x 20 sample, 1mm thick combs, casting dams, loading

guides

MSMAXIDUO 20 x 10 & 20 x 20cm UV Tray, 2 x 20 sample, 1mm thick combs, casting dams,

loading guides

MSMAXI25 20 x 25cm UV Tray, 2 x 20 sample, 1mm thick combs, casting dams, loading

guides

MS20-UV10 20 x 10cm UV Tray

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MS20-UV15 20 x 15cm UV Tray

MS20-UV20 20 x 20cm UV Tray

MS20-UV25 20 x 25cm UV Tray

MS20-UVDAM Casting Dams

MS15/20-FC multiSUB Choice/Maxi Flexi caster

MS20-LG Adhesive Loading Guides

MS20-WP Viewing Platform

MS20-PE Positive Electrode

MS20-NE Negative Electrode

MS20-UVS 20cm UV Gel Scoop

MSMAXICP Cool-Pack and Platform

MSMAXIBSB Buffer Saver Blocks pk/2 saves 450ml of buffer

Comb options

Catalogue No. Tank Thickness Sample Number Tooth Width (mm) Volume (ul)

MS7-1-0.75 Mini 0.75 1 45 152

MS7-2-0.75 Mini 0.75 2 20 68

MS7-4-0.75 Mini 0.75 4 10.63 36

MS7-8MC-0.75 Mini 0.75 8 2.5 8

MS7-8-0.75 Mini 0.75 8 5.5 19

MS7-10-0.75 Mini 0.75 10 4 14

MS7-12-0.75 Mini 0.75 12 3 10

MS7-16-0.75 Mini 0.75 16 2.2 7

MS7-1-1 Mini 1 1 45 203

MS7-2-1 Mini 1 2 20 90

MS7-4-1 Mini 1 4 10.63 48

MS7-8MC-1 Mini 1 8 2.5 11

MS7-8-1 Mini 1 8 5.5 25

MS7-10-1 Mini 1 10 4 18

MS7-12-1 Mini 1 12 3 14

MS7-16-1 Mini 1 16 2.2 10

MS7-1-1.5 Mini 1.5 1 45 304

MS7-2-1.5 Mini 1.5 2 20 135

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MS7-4-1.5 Mini 1.5 4 10.63 72

MS7-8MC-1.5 Mini 1.5 8 2.5 17

MS7-8-1.5 Mini 1.5 8 5.5 37

MS7-10-1.5 Mini 1.5 10 4 27

MS7-12-1.5 Mini 1.5 12 3 20

MS7-16-1.5 Mini 1.5 16 2.2 15

MS7-1-2 Mini 2 1 45 405

MS7-2-2 Mini 2 2 20 180

MS7-4-2 Mini 2 4 10.63 96

MS7-8MC-2 Mini 2 8 2.5 23

MS7-8-2 Mini 2 8 5.5 50

MS7-10-2 Mini 2 10 4 36

MS7-12-2 Mini 2 12 3 27

MS7-16-2 Mini 2 16 2.2 20

MS10-1-0.75 Midi 0.75 1 80 270

MS10-2-0.75 Midi 0.75 2 35 118

MS10-4-0.75 Midi 0.75 4 17 57

MS10-8-0.75 Midi 0.75 8 9 30

MS10MC-10-0.75 Midi 0.75 10 6 20

MS10-12-0.75 Midi 0.75 12 5 17

MS10-16-0.75 Midi 0.75 16 3.5 12

MS10-20MC-0.75 Midi 0.75 20 3 10

MS10-1-1 Midi 1 1 80 360

MS10-2-1 Midi 1 2 35 158

MS10-4-1 Midi 1 4 17 77

MS10-8-1 Midi 1 8 9 41

MS10MC-10-1 Midi 1 10 6 27

MS10-12-1 Midi 1 12 5 23

MS10-16-1 Midi 1 16 3.5 16

MS10-20MC-1 Midi 1 20 3 14

MS10-1-1.5 Midi 1.5 1 80 540

MS10-2-1.5 Midi 1.5 2 35 236

MS10-4-1.5 Midi 1.5 4 17 115

MS10-8-1.5 Midi 1.5 8 9 61

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MS10MC-10-1.5 Midi 1.5 10 6 41

MS10-12-1.5 Midi 1.5 12 5 34

MS10-16-1.5 Midi 1.5 16 3.5 24

MS10-20MC-1.5 Midi 1.5 20 3 20

MS10-1-2 Midi 2 1 80 720

MS10-2-2 Midi 2 2 35 315

MS10-4-2 Midi 2 4 17 153

MS10-8-2 Midi 2 8 9 81

MS10MC-10-2 Midi 2 10 6 54

MS10-12-2 Midi 2 12 5 45

MS10-16-2 Midi 2 16 3.5 32

MS10-20MC-2 Midi 2 20 3 27

MS15-1-0.75 Choice 0.75 1 110 371

MS15-2-0.75 Choice 0.75 2 50 169

MS15-4-0.75 Choice 0.75 4 27 91

MS15-10-0.75 Choice 0.75 10 10 34

MS15-10MC-0.75 Choice 0.75 10 6.5 22

MS15-12-0.75 Choice 0.75 12 9 30

MS15-14MC-0.75 Choice 0.75 14 6.5 22

MS15-16-0.75 Choice 0.75 16 6 20

MS15-20-0.75 Choice 0.75 20 4.75 16

MS15-28MC-0.75 Choice 0.75 28 2.5 8

MS15-35-0.75 Choice 0.75 35 2.2 7

MS15-1-1 Choice 1 1 110 495

MS15-2-1 Choice 1 2 50 225

MS15-4-1 Choice 1 4 27 122

MS15-10-1 Choice 1 10 10 45

MS15-10MC-1 Choice 1 10 6.5 29

MS15-12-1 Choice 1 12 9 41

MS15-14MC-1 Choice 1 14 6.5 29

MS15-16-1 Choice 1 16 6 27

MS15-20-1 Choice 1 20 4.75 21

MS15-28MC-1 Choice 1 28 2.5 11

MS15-35-1 Choice 1 35 2.2 10

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MS15-1-1.5 Choice 1.5 1 110 743

MS15-2-1.5 Choice 1.5 2 50 338

MS15-4-1.5 Choice 1.5 4 27 182

MS15-10-1.5 Choice 1.5 10 10 68

MS15-10MC-1.5 Choice 1.5 10 6.5 44

MS15-12-1.5 Choice 1.5 12 9 61

MS15-14MC-1.5 Choice 1.5 14 6.5 44

MS15-16-1.5 Choice 1.5 16 6 41

MS15-20-1.5 Choice 1.5 20 4.75 32

MS15-28MC-1.5 Choice 1.5 28 2.5 17

MS15-35-1.5 Choice 1.5 35 2.2 15

MS15-1-2 Choice 2 1 110 990

MS15-2-2 Choice 2 2 50 450

MS15-4-2 Choice 2 4 27 243

MS15-10-2 Choice 2 10 10 90

MS15-10MC-2 Choice 2 10 6.5 59

MS15-12-2 Choice 2 12 9 81

MS15-14MC-2 Choice 2 14 6.5 59

MS15-16-2 Choice 2 16 6 54

MS15-20-2 Choice 2 20 4.75 43

MS15-28MC-2 Choice 2 28 2.5 23

MS15-35-2 Choice 2 35 2.2 20

MS20-1-0.75 Maxi 0.75 1 150 506

MS20-2-0.75 Maxi 0.75 2 70 236

MS20-4-0.75 Maxi 0.75 4 34 115

MS20-10-0.75 Maxi 0.75 10 16 54

MS20-16-0.75 Maxi 0.75 16 9 30

MS20-20MC-0.75 Maxi 0.75 20 6 20

MS20-25-0.75 Maxi 0.75 25 4.7 16

MS20-30-0.75 Maxi 0.75 30 3.8 13

MS20-36-0.75 Maxi 0.75 36 3.2 11

MS20-40MC-0.75 Maxi 0.75 40 2.5 8

MS20-1-1 Maxi 1 1 150 675

MS20-2-1 Maxi 1 2 70 315

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MS20-4-1 Maxi 1 4 34 153

MS20-10-1 Maxi 1 10 16 72

MS20-16-1 Maxi 1 16 9 41

MS20-20MC-1 Maxi 1 20 6 27

MS20-25-1 Maxi 1 25 4.7 21

MS20-30-1 Maxi 1 30 3.8 17

MS20-36-1 Maxi 1 36 3.2 14

MS20-40MC-1 Maxi 1 40 2.5 11

MS20-1-1,5 Maxi 1.5 1 150 1013

MS20-2-1.5 Maxi 1.5 2 70 473

MS20-4-1.5 Maxi 1.5 4 34 230

MS20-10-1.5 Maxi 1.5 10 16 108

MS20-16-1.5 Maxi 1.5 16 9 61

MS20-20MC-1.5 Maxi 1.5 20 6 41

MS20-25-1.5 Maxi 1.5 25 4.7 32

MS20-30-1.5 Maxi 1.5 30 3.8 26

MS20-36-1.5 Maxi 1.5 36 3.2 22

MS20-40MC-1.5 Maxi 1.5 40 2.5 17

MS20-1-2 Maxi 2 1 150 1350

MS20-2-2 Maxi 2 2 70 630

MS20-4-2 Maxi 2 4 34 306

MS20-10-2 Maxi 2 10 16 144

MS20-16-2 Maxi 2 16 9 81

MS20-20MC-2 Maxi 2 20 6 54

MS20-25-2 Maxi 2 25 4.7 42

MS20-30-2 Maxi 2 30 3.8 34

MS20-36-2 Maxi 2 36 3.2 29

MS20-40MC-2 Maxi 2 40 2.5 23

15/11/2018 Page 25