Chemglass CLS-1445 User Manual

Chemglass Life Sciences

3800 North Mill Road

Vineland, NJ 08360 USA

Phone: [phone]

Email: [e-mail]

Web address: www.cglifesciences.com

CLS-1445 Series

Spinner Flask Instruction Manual

Rev. A 02/05/13

CLS-1445 Series Spinner Flask Instruction Manual

Description:

The Chemglass Life Sciences spinner systems are designed for long life, high
efficiency and low maintenance. Spinners are the ideal environment for culturing a
wide variety of cell cultures such as insect cells, hybridomas and many other
suspension adapted cells.

Material Specifications:

Spinner flasks are constructed of high quality components with the following
specifications:

Vessels: Borosilicate type 1 glass
Shafts: Borosilicate glass 7mm
Magnets: PTFE encapsulated
Cap: Polypropylene
Compression fitting: Polypropylene
Liner: Silicone
O-ring: Silicone

Material certifications: 21 CFR 177.1550
21 CFR 177.2600
TSE\BSE Declarations
ASTM E-438, USP Type 1, Class A glass

Material certifications are available upon request.

Features:

All sizes feature a flat bottomed vessel
Heavy wall center port and side arms
FDA approved materials

The warning symbol indicates attention to an operation that could
cause injury, equipment or process damage.

Manual Symbols:

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CLS-1445 Spinner Manual CG Life Sciences

Operating and Assembly Instructions:

The cap and magnet assemblies are shipped partially assembled.

Glass Rod Installation (refer to Figure 1):

Remove the magnet from the magnet holder by pushing the magnet through the
holder until free. Insert the glass shaft into the hole in the bottom of the magnet
holder and re-install the magnet.

WARNING: Be careful handling the glass shaft as accidental
breakage can cause injury

Figure 1

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CLS-1445 Spinner Manual CG Life Sciences

Magnet Assembly Installation (refer to Figures 2 & 3):

Loosen the cap nut from the cap but do not completely remove. For easier
installation, moisten the glass shaft with water and gently slide it through the cap
assembly from the bottom through compression fitting body and then hand tighten
the cap nut loosely to hold the magnet assembly in place.

Place the entire assembly into the flask and adjust the height of the magnet by
sliding the glass shaft either up or down until the desired height is achieved (see
figure 3). Next tighten the cap nut to lock the magnet height.

Figure 2

Figure 3

WARNING: Be careful handling the glass shaft as accidental
breakage can cause injury

¼” to ½”

From
magnet
holder to
bottom

With magnet
assembly attached
(from Figure 1)

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100mL, 250mL & 500mL Spinners

CLS-1445 Spinner Manual CG Life Sciences

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1L & 3L Spinners

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NOTE: THE MAGNET HOLDER (P/N’S: 1445-001 and 1445-002)
WILL WEAR OVER TIME AND SHOULD BE INSPECTED AND
REPLACED PERIODICALLY

Cleaning:

Use a commercial cleanser approved for glass. Chemglass Life Sciences
recommends item number CLS-4752, 7X™ and 7X-O-Matic® cleaning solutions.

Rinse all glassware as soon as possible after use.
To decontaminate ware, autoclave contaminated items in water containing

cleanser.
Disassemble spinner assemblies, compression fittings and paddle assemblies for

cleaning and to avoid the trapping of disinfectants and cleansers. Failure to do so
may result in the release of these compounds during use with possible detrimental
effects to cultures or assays.

Thoroughly rinse items in tap water then distilled or deionized water - even the
smallest products. Rigorously follow your rinsing protocol.

Do not use glassware that is scratched, chipped or cracked for centrifugation,
pressure, vacuum, heating or freezing. Breakage may result.

Sterilizing:

To prevent warping of the top cap and magnet holder, it is recommended that the
flask be autoclaved in the standing position only. In large vessels, place a few ml of
distilled water in the flask to generate additional steam in the vessel. Place
approximately 20 ml of water (or PBS buffer) for each 10 liter of flask volume and
autoclave @ 121C for 15 to 30 minutes.

Make sure that the vessel is vented so that pressure or vacuum
will not build up and cause vessel to explode or implode.

Chemglass Life Sciences Warranty and Limitation of Liability

Guarantee:

Chemglass Life Sciences, herein known as CGLS, warrants the equipment to be free
from all defects and guarantees acceptable performance assuming the equipment has
been installed in accordance with the instructions provided by CGLS, and has been
used for the specific design purpose, and has been maintained according to the
maintenance instructions accompanying the equipment or other CGLS published
documents. The warranty period is for one year from the date of sale. If the unit is
determined to have a defect in materials or workmanship, then it will be repaired or
replaced at no charge.

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CLS-1445 Spinner Manual CG Life Sciences

Return Authorization:

CGLS must authorize any return of material. Please contact a customer service
representative at the correspondence listed below to obtain a Return Merchandise
Authorization (RMA) number. The purchaser is responsible for all packing and
shipping to CGLS. If the equipment or material came in contact or was proximate to
any biological organism, toxic or corrosive material, or any agent reasonably deemed
to be potentially harmful, it must be cleaned and decontaminated prior to receipt by
CGLS. The purchaser is obligated to disclose fully in writing, the cleaning and
decontamination method. We reserve the right not to accept any unauthorized or
potentially harmful shipment.

Correspondence:
Chemglass Life Sciences
3800 North Mill Rd.
Vineland, NJ 08360

USA
Phone: [phone]
Email: [e-mail]
Web: www.cglifesciences.com

CLS-1445 Spinner Manual CG Life Sciences

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