Centra CL-2 User manual

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Page 1

INSTRUCTION MANUAL

IM-426

Revision 8

IEC Centra

INTERNATIONAL EQUIPMENT COMPANY

CL2

Centra-CL2

Centrifuge

Cat. No. 426 -- For 120 VAC, 50/60 Hz Cat. No. 427 -- For 240 VAC, 50/60 Hz

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Table of Contents

1 INTRODUCTION.............................................................................................. 2

2 INSTALLATION................................................................................................ 3

3 OPERATION .................................................................................................... 4

3.1 Warnings and Cautions ............................................................................ 4

3.2 Opening The Cover .................................................................................. 4

3.3 Rotor Installation...................................................................................... 5

3.4 Starting And Stopping A Run.................................................................... 5

3.5 Rotor Removal.......................................................................................... 5

3.6 Balance..................................................................................................... 6

4 APPLICATIONS ............................................................................................... 7

4.1 Speed And Force Tables .......................................................................... 8

4.2 Derating Table for Dense Samples........................................................... 10

4.3 Chemical Resistance Table ...................................................................... 11

4.4 Compatible Processes For Decontamination............................................ 12

4.5 Nomograph ............................................................................................... 13

5 MAINTENANCE ............................................................................................... 14

5.1 Cleaning.................................................................................................... 14

5.2 Brush Replacement .................................................................................. 15

5.3 Fuse Replacement.................................................................................... 16

5.4 Cover Interlock Bypass............................................................................. 16

5.5 Calibration................................................................................................. 16

5.6 Power Cord Replacement......................................................................... 16

5.7 Warranty ................................................................................................... 17

5.8 Condition of Returned Equipment............................................................. 17

6 SPECIFICATIONS ........................................................................................... 18

7 SERVICE ......................................................................................................... 19

7.1 Warning Messages and Error Codes....................................................... 19

7.2 General ..................................................................................................... 19

7.3 Disassembly For Service .......................................................................... 20

7.4 Interlock .................................................................................................... 20

7.5 Timer PCB ................................................................................................ 21

7.6 Motor......................................................................................................... 21

7.7 Interlock PCB............................................................................................ 22

8 DRAWINGS ..................................................................................................... 23

IMPORTANT

This manual may not contain information on all changes that have occurred to the subject instrument since the manual issue date. It was prepared for use by IEC authorized factory-trained service or dealer personnel who are kept current through a program of service letters and bulletins and training seminars.

This manual contains personal injury. It also contains centrifuge or accessory equipment. Read this manual thoroughly before operating or servicing this centrifuge.

Centra-CL2 Instruction Manual

warnings

against operating procedures which could result in an accident and/or

cautions

against procedures which could result in damage to your

Page 3

1 INTRODUCTION

The IEC Centra-CL2 is a compact benchtop centrifuge designed for multipurpose use in medical, industrial, and scientific laboratories.

There are two models:

• Cat. No. 426 for 120 V, 50/60 Hz

• Cat. No. 427 for 240 V, 50/60 Hz

The Centra-CL2 accommodates a wide variety of rotors, including fixed angle and horizontal (swinging bucket). IEC AeroCarriers™ provide aerosol containment along with autoclaveability. The CL2 can handle up to 300 ml (801 rotor), and reach maximum speeds of up to 8500 rpm (841 fixed angle rotor) and 3900 rpm (236 horizontal rotor).

Other important features of the CL2 are a cover interlock for safety, and a 'glove-friendly' membrane control panel with digital speed and time displays. An 'At Speed' timer mode allowing for accurate separations, and a continuous 'hold' mode are also featured.

Centra-CL2 Instruction Manual

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2 INSTALLATION

After unpacking, place the unit on a clean, level surface. The surface must be level to ensure quiet, vibration-free operation. A rigid, stable location is important since an improperly loaded unit can vibrate or even move. Allow a space of 3 in. (7.6 cm) on each side and 4 in. (10.2 cm) in the rear of the unit for ventilation. Ensure that the suction cup feet grip the surface firmly.

Using a voltmeter, measure the line voltage to ensure it is within the limits for your model. For Cat. No. 426 the line voltage should be between 108 and 132 VAC. For Cat. No. 427 the line voltage should be between 216 and 264 VAC. Variations in line voltage or frequency will affect the unit’s speed and acceleration.

Before moving, unplug the centrifuge and remove all accessories and the rotor.

Clearance Envelope International Electrotechnical Commission standard 1010 part 2-20 limits

the permitted movement of a laboratory centrifuge to 300mm in the event of a disruption. The user should therefore mark the clearance envelope boundary around the centrifuge, or laboratory management procedures should require that no person or any hazardous materials are within such a boundary while the centrifuge is operating.

Centra-CL2 Instruction Manual

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3.1 Warnings and Cautions

Warnings To Avoid Electric Shock:

• Plug the power cord into a grounded outlet.

• Never remove the grounding prong from the power plug, or use any adapter which does not complete the grounding circuit.

3 OPERATION

• Always unplug the power cord before attempting to clean or service

Cautions

• DO NOT exceed maximum rated speed for each rotor/accessory

• Samples of specific gravity higher than 1.2 require the maximum

• Ensure that loads are properly balanced around the rotor to minimize

• Do not block the vents, otherwise, airflow will be restricted.

• Be sure the rotor and accessories are properly installed before

3.2 Opening The Cover

Once the red light over the STOP button is steadily illuminated (no longer flashing) pressing the COVER OPEN button on the control panel will release

STOP/COVER

OPEN

the interlock, allowing the cover to be opened.

the centrifuge.

combination. Maximum speeds can be found in Section 4.1 Speed And Force Tables. All IEC rotors and accessories are stamped with their cat. no. for easy identification.

speed to be derated. vibration. All IEC accessories are stamped with their weight for easy

balancing.

attempting to start a run.

Centra-CL2 Instruction Manual

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3.3 Rotor Installation

When the unit has power, the red light over the STOP button is illuminated. This is also an indication that the rotor is stopped and the cover can be opened. Push the COVER OPEN lever to the right and lift the cover. Lower the rotor straight onto the shaft. Screw the knurled metal locking nut (clockwise) onto the shaft to hold the rotor down (on some rotors, you must remove any sample tubes first.). Tighten the nut with your fingers; do not use a tool.

Rotors with or without a keyway can be used on the Centra-CL2 since there is no key on the shaft.

3. 4 Starting And Stopping A Run

To start a run, use the ARROW buttons to set the desired run time (0 to 30 minutes) in the TIME display, and the desired rpm in the SPEED display. Press the START button. The green light under the START button will illuminate, and the time display will begin counting down. The actual speed is

ARROW

displayed in the SPEED display. The centrifuge will run for the set duration and decelerate to a stop. To terminate a run before time expires, press the STOP button. The red light over the STOP button illuminates when STOP is pressed or time expires, and it flashes until the rotor comes to a stop.

TIME

SPEED

START

STOP

3.5 Rotor Removal

Note: The cover may be opened when the rotor speed is below 20 RPM. The time and speed settings cannot be changed during a run. A new run

cannot be started until the rotor has come to a complete stop. For infinite spins (hold mode), use the arrow keys to scroll up past 30 minutes.

The word 'HOLD' appears in the display. Pressing the START button will begin a run which can only be terminated by pressing the STOP button. In the hold mode, the timer counts up.

To select the timing mode, use the arrow buttons to scroll down past 0 seconds. The letters 'Spd' or 'Acc' will appear. Press the arrow buttons to toggle between the two timing modes. 'Spd' is the 'At Speed' timing mode where the timer starts counting down when rotor reaches 95% of set speed. 'Acc' is the normal timing mode where the timer begins to count down as soon as the run button is pressed.

To remove a rotor, first remove any sample tubes, shields, and other accessories from the rotor. Next, unscrew (counterclockwise) approximately one full turn the knurled locking nut. Then place both thumbs on the knurled locking nut and grip the rotor with the fingers. Push your thumbs down and at the same time pull the rotor up with your fingers. This should dislodge the rotor from the shaft. If unsuccessful, lightly tap the knurled metal locking nut with a rubber/plastic mallet or other similar object. The nut and the rotor can now be removed from the shaft.

Centra-CL2 Instruction Manual

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3.6 Balance

A balanced load is essential with all centrifuges. An unbalanced load

produces vibration and can damage the unit. A 2-gram load imbalance, at a speed of 4600 rpm, imparts force equivalent to 9.1 kg at rest (20 pounds). Therefore, always ensure that the rotor is loaded symmetrically and with a full (or paired) set of tubes. Tube adapters should also be installed symmetrically.

IEC rotors are dynamically balanced at the factory. IEC matches removable parts (trunnion rings, shields, cups and carriers) to within 1 gram and stamps the weight on each piece. Check these markings whenever you interchange parts, to ensure that opposite parts are matched. Ensure that the total weight of samples and removable parts loaded in opposing positions are equal in weight to within 1 gram. The position numbers, present on many rotors and adapters, identify opposing tube positions.

To obtain good dynamic balance, the opposite loads must not only be equal in mass, but must also have the same center of gravity. Opposing containers must be alike in shape, thickness, and distribution of glass or plastic. This is especially important for large containers.

Tubes loaded into swinging bucket rotors must likewise be symmetric around the axis of rotation. Verify this by rotating the entire rotor 180° by hand: the loads should be in the same apparent positions (not in the mirror image). In addition, the loads within each bucket must also be symmetric around the bucket’s pivot axis. Verify this by ensuring that each bucket is loaded so that it does not tilt from the vertical when the rotor is at rest. Maintaining balance within each bucket ensures that the bucket and the tubes swing out to horizontal when the rotor reaches operating speed, applying centrifugal force toward the bottom of the tubes. Failure to achieve full swing-out causes vibration and premature wear.

Samples of different specific gravities can be processed in the same run, provided that the samples of a given type are balanced around the rotor as

though they were the only ones in the rotor.

For example, load tubes in the following manner:

1. Load four tubes. Positions 3, 6, 10, 13 or 2,5,9,12 or 1,4,8,11

2. Load six tubes. Positions 6,7,3,13,14,10 or 5,7,2,12,14,9 or 1,7,4,8,14,11

3. Load an odd number of tubes. Not recommended (unless a dummy tube is used for balance.)

Centra-CL2 Instruction Manual

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4 APPLICATIONS

This section describes the use of specific rotors and accessories. More detailed information is often shipped with the rotor or accessory itself. This section contains four reference tables:

4.1 Speed And Force Table

4.2 Derating Table for Dense Samples

4.3 Chemical Resistance Table

4.4 Decontamination Table

4.5 Nomograph Relative centrifugal force (RCF or G-force) at a given speed varies with the

rotor, and with the length of the sample tube, because the distance of the tube’s tip from the center of rotation is different. The Speed and Force Table indicates the maximum speed and RCF the Centra-CL2 can achieve with various rotor/accessory combinations.

The Derating Table specifies reductions in rpm when spinning samples with specific gravity above 1.2.

Misapplication of any tube can cause tube rupture. To avoid this, compare the G forces specified in the Speed and Force Table with the ratings for the tubes you are using. If the tubes are not rated for the force the centrifuge will apply, reduce the speed to the G force limit of your tubes.

Your IEC centrifuge is made of materials designed to resist attack from most laboratory chemicals. The interior of the rotor chamber is Painted steel. Rotors and accessories placed in the chamber are made of a variety of materials, including aluminum and polypropylene. The Chemical Resistance Table shows the suitability of each material with different classes of reagents.

The Decontamination Table lists compatible methods of decontamination which may be used on the IEC Centra-CL2 centrifuge.

The Nomograph provides an easy method of converting RPM to RCF (or xg).

Section 5.1 describes how to clean and remove corrosion from the chamber, rotors, and accessories. Follow these instructions, and clean spills promptly, to minimize the effects of corrosive chemicals, before any resulting chemical attack requires more expensive repair. Replace metal locking nut, rotors, or accessories if they become cracked, deformed, or gouged.

Centra-CL2 Instruction Manual

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4.1 Speed And Force Tables

Rotor 215: 4-Place Swinging Bucket Rotor

Tube

No. x Vol. Tube

8x50ml

8x15ml

4x50ml

4x15ml

4x50ml sealed

4x15ml sealed

4x10-15ml sealed

4x7ml sealed

12x10ml

12x7ml

16x7ml

16x5ml

20x5ml

20x3ml

Falcon/Corning Conical plastic

Corning 8300-50 conical glass

Falcon/Corning Conical plastic

Corning 8300-50 conical glass

Falcon/Corning Conical plastic

Falcon/Corning

Vacutainer 16x100-125mm

Vacutainer 13x100mm

16x100mm

16x75mm

13x100mm

13x75mm

12x75mm

10x75mm

Maximum

RPM / RCF Radius

3100 / 1510

3350 / 1750

3175 / 1750

3350 / 1750

3175 / 1710

3176 / 1710

3175 / 1710

3450 / 1725

3450 / 1700

4000 / 1975

14.1

13.9

15.4

13.9

15.2

12.9

12.8

11.1

Tr.

Ring

4x326

4x325

4x350

4x325

4x350

4x366

Shield or

Carrier Adapter Cushion

8x320

4x320

4x323

4x320

4x7323

4x1013

4x1018

4x369

8x1106

4x1106

1106

4x1105

8x315

571

570

4x315

571

570

315

4x668

4x571

4x668

4x571

570

667

567

Rotor 221: 6-Place Fixed Trunnion Swinging Bucket

Tube

No. x Vol. Tube

6x15ml

6x12.5ml

6x12ml

6x10ml

Falcon/Corning

Kimble 45170-125

IEC 1629, 1649

Corning 8080-10

IEC 2046, 2067

Maximum

RPM / RCF Radius

3100 / 1650

3100 / 1570

3500 / 1890

3500 / 1780

15.4

14.6

13.8

13.0

Tr.

Ring

Fixed

Shield or

Carrier Adapter Cushion

6x303

6x356

Rotor 236: 4-Place Aerocarrier Horizontal Swing-Out Rotor

Tube

No. x Vol. Tube

4x50ml Falcon/Corning 3400 / 1950 15.0 Fixed 4x2091S - -

8x15ml

8x10ml

16x10ml

16x7ml

28x7ml

28x5ml

Falcon/Corning

Kova/UriSystem

Vacutainer 16x100mm

Vacutainer 13x100mm

Hemogard Vacutainer 13x100mm

Vacutainer 16x75mm

Vacutainer 13x75mm

Hemogard Vacutainer 13x75mm

Maximum

RPM / RCF Radius

3400 / 2000

3700 / 2200

3701 / 2200

3700 / 200

3900 / 2150

3901 / 2150

3900 / 2150

15.5

14.3

12.7

Tr.

Ring

Fixed

Shield or

Carrier Adapter Cushion

4x2092S

4x2093S

4x2094S

668

570

668

570

8 Centra-CL2 Instruction Manual

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Rotor 801: 6-Place 45 Degree Fixed Angle Rotor

Tube

No. x Vol. Tube

6x50ml

6x15ml

6x50ml

6x15ml

Falcon/Corning Conical plastic

Corning 8300-50 conical glass

Falcon/Corning Conical plastic

Corning 8300-50 conical glass

Falcon/Corning Conical plastic

Maximum

RPM / RCF Radius

3900 / 2050

4500 / 2450

12.1

10.8

Tr.

Ring Shield Adapter Cushion

6x305

6x320

6x1106

Rotor 804S: 4-Place 40 Degree Fixed Angle Rotor

Tube

No. x Vol. Tube

4x50ml

4x15ml

4x60ml Corning 8540-60 2500 / 1000 14.3 - 4x341 - 572

Falcon/Corning Conical plastic

Corning 8300-50 conical glass

Falcon/Corning Conical plastic

Maximum

RPM / RCF Radius

4200 / 2270

11.5

Tr.

Ring Shield Adapter Cushion

323

6x1106

Rotor 809: 12-Place 45 Degree Fixed Angle Rotor

Tube

No. x Vol. Tube

12x15ml

126xDevice

12x10ml

12xDevice

12x10ml

Falcon/Corning conical plastic

Corning 8080-15 conical glass

Amicon Filtration Device

Corning 8080-10

Filtron or Millipore Devices

Corning 8080-10

17x102mm

Maximum

RPM / RCF Radius

3800 / 2150

3800 / 2050

4500 / 2310

4500 / 2490

4100 / 2270

4100 / 2120

13.3

12.7

13.4

10.2

11.0

12.1

11.3

Tr.

Ring Shield Adapter Cushion

302

12x356

12x303

6x315

571

570

6x315

571

570

315

571

570

668

570

12x668

570

Rotor 841: 12-Place 45 Degree Fixed Angle Rotor

Tube

No. x Vol. Tube

12x1.5-2.0ml

12x0.7ml

12x0.5ml

12x0.4ml

12x0.25ml

microtubes

Maximum

RPM / RCF Radius

8500 / 4680

8500 / 4770

8500 / 3960

8500 / 4680

8500 / 3630

5.8

5.9

4.9

5.8

4.5

Tr.

Ring Shield Adapter Cushion

5763

5764

Centra-CL2 Instruction Manual 9

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4.2 Derating Table for Dense Samples

The Speed and Force Table lists the Maximum speed for each rotor/ accessory combination in the Centra-CL2. IEC guarantees that the units can achieve these speeds when used at nominal voltage.

These speeds are guaranteed only with samples whose specific gravity is not greater than:

1.2 for swinging bucket rotors

1.5 for angle rotors

For denser samples, the maximum guaranteed speed is reduced (derated) by a factor from the table below:

Specific Gravity Swinging Bucket Fixed Angle

1.2 1 1

1.3 .960 1

1.4 .925 1

1.5 .894 1

1.6 .866 .967

1.7 .839 .939

1.8 .816 .912

1.9 .794 .888

2.0 .774 .866

2.1 .755 .844

2.2 .738 .825

2.3 .721 .807

2.4 .707 .790

2.5 .692 .774

2.6 .678 .758

2.7 .666 .744

2.8 .654 .731

2.9 .642 .719

3.0 .632 .707

Derating Factor for:

Example. An angle rotor rated for 5,000 rpm, used with samples with a

specific gravity of 1.6, should not be spun faster than (5,000 x .967 =) 4,835 rpm.

Specific gravities greater than 3.0. This table is based on the formula:

Ö(s0/sa)

...where s0 is the maximum specific gravity allowed before derating (1.2 or

1.5, depending on the type of rotor), and sa is the actual specific gravity of the sample in question. You can use the same formula to compute derating factors for specific gravities greater than 3.0.

Centra-CL2 Instruction Manual

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4.3 Chemical Resistance Table

Centra-CL2 Instruction Manual

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4.4 Compatible Processes For Decontamination

WARNING:

This chart describes the material compatibility of various sterilization methods. It does not specify the adequacy of sterilization. Refer to section 4.3 Chemical Resistance Table for material compatibility during centrifugation.

Centra-CL2 Instruction Manual

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4.5 Nomograph

Centra-CL2 Instruction Manual

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5.1 Cleaning

Corrosion IEC manufactures and finishes rotors and structural accessories to give

5 MAINTENANCE

Keep your centrifuge clean to ensure good operation and to extend its life. Clean the entire sample chamber, rotor, and lid at the end of each workday, and also right after any spill.

To clean the sample chamber, use a damp sponge, warm water, and a mild liquid detergent suitable for washing dishes by hand, such as Ivory® liquid. Do not use caustic detergents or detergents that contain chlorine ions, since these attack metals. Remove stubborn stains with a plastic scrub pad. Do not use steel wool, wire brushes, abrasives, or sandpaper, since they create corrosion sites. Never pour water directly into the sample chamber. Scrub the rotor’s tube cavities with a stiff test-tube brush that has end bristles and a nonmetallic tip. After cleaning any part, dry it properly, preferably using a clean, absorbent towel.

maximum resistance to corrosion. However, maximum equipment life requires that you continually inspect the rotor cavities for corrosion, especially after using chloride ion solutions, such as sodium chloride (saline), and sodium hypochlorite (household bleach). These solutions attack most metals. Clean the rotor, rotor chamber, and accessories (particularly the sample compartments and bucket cups) thoroughly after each such use. Inspect all surfaces under bright light for corrosion; small crevices will grow deeper and cause failure.

If you see any corrosion, remove it immediately as follows:

1. Follow the cleaning procedure at the start of this section. Soak the part in the mild hand-dishwashing detergent. Scrub the part thoroughly with a stiff test-tube brush having end bristles and a nonmetallic tip.

2. Soak the part again in clear warm water for at least an hour.

3. Rinse the part thoroughly in warm water first, then in distilled water.

4. Dry the part thoroughly with a clean, absorbent cloth.

5. If this procedure does not remove the corrosion, discontinue use of

the part.

Storage Store parts on a soft surface to avoid damaging finished surfaces. Rotors

and other parts should be clean and dry for storage. Store them open to the atmosphere, not in a plastic bag, so that any residual moisture will evaporate. The parts should face downward to avoid retaining moisture in the cavities.

Centra-CL2 Instruction Manual

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Decontamination Decontamination is called for if tube breakage occurs and infectious,

pathogenic, or radioactive material is released into the unit. Some rotors or accessories totally contain the sample tubes. In this case, spillage is usually confined, and it may be sufficient to decontaminate only the rotor or accessory.

The Decontamination Table lists the sensitivity of various materials to common sterilization procedures. When using a 1-to-10 dilution of household bleach (sodium hypochlorite) to decontaminate metal rotors or accessories, follow decontamination by the corrosion cleaning procedure given earlier, since chloride ions attack most metals.

Always decontaminate for the minimum recommended time. If you observe corrosion, remove it as described earlier, discontinue use of the sterilization method, and use an alternate decontamination procedure.

Sterilization of polypropylene rotors can be done by autoclaving. Remove any sample tubes before autoclaving, unless they are completely full of sample, or remove caps, stoppers, and other tube closures, before autoclaving to keep the tubes from collapsing under pressure. Autoclave the rotor and accessories at 121° C @ 15 psig for 20 minutes. Do not stack polypropylene rotors during autoclaving. After the rotor is cool to the touch, do a normal cleaning operation as described above.

Repeated autoclaving will seriously degrade the performance of polycarbonate materials.

5.2 Brush Replacement

Refer servicing to qualified personnel only. Brush replacement is required when the length of the brush not including the spring is less than 1/4 inch long.

Order additional sets as IEC Part Number 1780A.

WARNING DISCONNECT POWER CORD BEFORE REMOVING THE

First, remove all rotor and accessories from the chamber. Gently tilt the unit onto its side and remove the four screws which secure the baseplate. The brush caps are located on either side of the motor housing. Unscrew each cap with your fingers (or use a small flat screwdriver) and remove the brushes. There are two black caps which can be removed from the housing to allow use of a large screwdriver. Measure the length of the brushes and replace both brushes if either one is less than 1/4 of an inch long. Be sure to reinstall all parts removed.

It is important to check the brushes periodically since damage to the motor can occur if the brush is allowed to wear down to the spring.

BRUSHES.

Centra-CL2 Instruction Manual

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CAUTION: WHEN REINSTALLING INSPECTED BRUSHES When brush replacement is not required, it is important that the brush be inserted in the same position as it was removed. The trailing edge of the brush must be positioned properly. The trailing edge may be identified by the presence of a dark deposit of carbon along that side.

Note: New brushes may require a burn-in period of up to a half hour.

5.3 Fuse Replacement

Refer servicing to qualified personnel only. First, remove all rotor and accessories from the chamber. Gently tilt the unit onto its side and remove the four hex head screws which secure the baseplate. Unscrew and remove the four rubber feet and lift the baseplate off. The fuse(s) is mounted to the cabinet housing. Replace fuse(s) with:

For 100/120 VAC 1 - 4A, .25 x 1.25 in. IEC part no. 40340 For 220/240 VAC 2 - 2A, .25 x 1.25 in. IEC part no. 40794

5.4 Cover Interlock Bypass

The Centra-CL2 has an interlock bypass for easy sample retrieval in the event of a power failure. To bypass the safety interlock, unplug the centrifuge and pry off the plastic plug located on the bottom of the control panel. Pull downward on the cord to release the interlock. Do not perform this bypass routinely. The cover interlock provides user safety and allows the cover to be opened promptly whenever rotation has stopped.

5.5 Calibration

The speed sensor used in the Centra-CL2 requires no calibration. IEC recommends verifying its speed once every 24 months. This can be done easily using an optical tachometer through the clear plastic viewport in the lid. If this measurement indicates instrument failure, please notify IEC Technical Service.

5.6 Power Cord Replacement

Inspect the power cord every four months for signs of wear. Refer servicing to qualified personnel only. Replace power cord with IEC part number 44392 only.

Centra-CL2 Instruction Manual

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5.7 Warranty

Warranty information is provided on the warranty card supplied with the centrifuge.

5.8 Condition of Returned Equipment

Before returning equipment to IEC, you must contact IEC or your dealer and receive a return goods authorization (RGA). All returned units must be

decontaminated, free of radioactivity, and free of hazardous and infectious materials. The RGA paperwork includes a Certificate of

Decontamination for you to sign indicating that you have performed these steps. IEC will not accept the shipment until this signed certificate is received.

You must prepay transportation to the service depot.

Centra-CL2 Instruction Manual

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6 SPECIFICATIONS

Rotation Speed 8500 rpm (Angle Rotor No. 841)

3900 rpm (Horizontal Rotor No. 236)

Maximum RCF 4775 xg (Angle Rotor No. 841)

2200 xg (Horizontal Rotor No. 236) Maximum Volume 400 mL (8 x 50 mL) Timer

Range 0 to 30 minutes Increments 0 to 1 minute by 5 seconds

1 to 5 minutes by 15 seconds

5 to 30 minutes by 1 minute

HOLD mode up to 99 min., 99 sec.

Accuracy ±1.0 %

Speed

Range 1000 to 8500 rpm by 100 rpm Accuracy ±100 rpm

Power Requirement 120 VAC ± 10%, 60 Hz (Cat. No. 426)

240 VAC ± 10%, 50 Hz (Cat. No. 427) Heat Output (typical) 175 Watts (600 Btu/hr.) Sound Level 65 dB(A) Height

Cover Closed: 11 in. (28 cm)

Cover Opened: 24 in. (61 cm) Width 13 in. (34 cm) Depth 16 in. (40 cm)

Net Weight 11 kg (24 lbs.) Shipping Weight 13 kg (29 lbs.)

Specifications Subject To Change Without Notice

Centra-CL2 Instruction Manual

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7.1 Warning Messages and Error Codes

The following Warning Messages and Error Codes can appear in the display of the Centra-CL2. A Warning Message indicates improper opeartion, and may be cleared by opening the lid, correcting the problem, and then using the centrifuge. Error Codes indicate a malfunction of the centrifuge. They are cleared by disconnecting and reconnecting power to the centrifuge. If an Error Code or Warning Message persist, service may be required.

Warning Messages: LId: The cover was not properly closed when the start button was

pressed, or the cover was opened during a run.

PFL: Power to the centrifuge was lost during a run.

Error Codes:

7 SER VICE

7.2 General

Trouble Shooting If the centrifuge won’t start, check the following in order to isolate a problem:

Warnings: The following hazards exist in servicing the Centa-CL2:

Er 1: Tachometer signal not present during a run. Er 2: Speed is 500 RPM over set speed and not decelerating for more

than 2 seconds, or speed is over 9000 RPM at any time.

Er 10: The motor voltage was detected to be over the maximum limit

(94 volts).

If the rotor stopped indicator (red LED) is lit, there is power to the centrifuge. If not, unplug the centrifuge and check the fuse(s) per Section 5.3.

Spin the rotor by hand to see that the rotor stopped indicator begins flashing, and listen for the latch to engage. This will verify the function of the tachometer and latch.

Centra-CL2 Instruction Manual

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The unit uses AC power, and some of the service procedures require operation

with panels removed, exposing power lines. This introduces the risk of electric

shocks. Service should be performed by qualified personnel only. Do not

touch exposed wires without first unplugging the unit.

Cautions: An additional hazard to the equipment is as follows:

The circuit boards contain electronics that can be damaged by static

electricity. Persons doing extensive maintenance on the circuit boards, or

removing individual components from the circuit boards, should be grounded

(such as by wearing a wrist strap.) When shipping a circuit board, always

enclose it in a static-protective bag.

7.3 Disassembly For Service

First, open the cover and unplug the centrifuge. To access the

electrical components, remove the rotor and accessories from the chamber.

Gently tilt the unit onto its side and remove the four hex head screws which

secure the baseplate. Unscrew and remove the four rubber feet and lift the

baseplate off.

With the cover open, remove the four screws (two on each side) from the

control tower. Gently pull the tower outward without removing it completely

(there are wires connecting it to the chamber).

7.4 Interlock

To remove the guard bowl, locate and remove the 9 Phillips head screws at the

base of the centrifuge chamber. To remove the cover from the guard bowl, pry

off one end cap from the hinge pin. Slide the pin out.

The IEC Centra-CL2 has a safety interlock which prevents the cover from being

opened unless the rotor is turning 20 RPM or less. There is a switch in the

interlock assembly which senses that the cover is closed and locked. It is a

normally open switch and closes when the solenoid is in the locked position.

When pressed, the STOP/COVER OPEN button releases the safety

interlockby powering the solenoid through the Interlock PC board.

The solenoid coil has a resistance of approximately 80 W. Power to the

solenoid should be approximately 170 VDC. Voltage is provided by the

Interlock PCB when the STOP/COVER OPEN button is pressed.

Note: The following procedure involves operating the unit with panels

removed. Refer to Section 7.1 Warnings.

To measure the voltage, disconnect the solenoid at JP4 and read across the

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7.5 Timer PCB

BRN and YEL leads. Verify the function of the switch using an ohmmeter. To replace the interlock, remove the four screws which secure the latch

assembly to the cabinet. Snip the wire ties in order to disconnect all leads to the latch assembly. Make note of wiring before disconnecting. Reconnect wires as noted, or using the diagram provided at the end of this manual.

The slots in the latch assembly mounting bracket are for proper positioning. Raise or lower the height of the assembly so that when the cover is closed completely switch SW1 (WHT and WHT/BLK leads) is engaged by the roll pin of the solenoid plunger. A run may not be started until the switch is engaged.

The IEC Centra-CL2 has a Timer/Display PCB mounted behind the membrane control panel. The timer PCB contains the EPROM and delivers power to the motor. To replace it, the tower must first be removed (see Section 7.2). The PCB is secured to the tower by four screws

Note: To prevent damage to the PC board, always use a static protective device (such as wriststrap) when handling or servicing.

7.6 Motor

The IEC Centra-CL2 uses an AC series wound drive motor. The motor is not a singular replaceable component, as the base housing of the centrifuge serves as the motor housing. The motor is made up of an armature (includes bearings), field, brushes, brush holders, brush caps, brush leads, and a magnetic rotor. All of these parts are available separately (as well as motor bearings) for repair purposes.

When isolated, the armature resistance should be approximately 6.3 W. This can be measured by disconnecting the RED and WHT motor leads and measuring the resistance across them. When isolated, the field resistance should be approximately 10 W. It can be measured by disconnecting the Red and BLK motor leads (BLK at BR1).

To access the motor, remove the baseplate (see Section 7.2) and then the top cap located in the guard bowl. Two Phillips head screws secure the top cap. When removing the top cap, take care not to lose the pre-load washer which rests on top of the upper bearing.

To replace the armature, the brushes must be removed (see Section 5.2), and the magnetic rotor taken off of the motor shaft. Magnetic rotor removal requires that the Interlock PCB be removed (see Section 7.6). It is secured with Loctite® 454. Use care not to break the magnetic rotor. Once brushes and the magnetic rotor have been removed, the armature simply lifts out of the housing.

The field rests inside the housing. To remove it, simply disconnect the three

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motor leads (RED, WHT, BLK) and lift it out.

Located below the field are the two set screws which secure the brush holders

in place. To replace or adjust a brush holder, loosen the set screw. This

allows the brush holder to be moved or removed. The set screw may be

accessed through the two holes in the field through which the top cap

mounting screws secure the top cap to the base.

7.7 Interlock PC Board

The Interlock PCB in the IEC Centra-CL2 disables power to the interlock during

rotation. This PC board is mounted below the motor. To sense rotation, it uses

a Hall effect sensor to pickup pulses form a magnetic disk mounted on the

motor shaft.

To replace the PCB, disconnect the wiring harness and remove the four

mounting screws which secure the PCB to the base housing. Take care not to

damage the speed sensor when lifting the PCB off of the housing.

Note: To prevent damage to the PC board, always use a static protective

device (such as wriststrap) when handling or servicing.

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8 DRAWINGS

The following drawing are included in this manual.

Dwg. No. Description

42 6 CL2 Assembly PL-426 CL2 Parts List (120 VAC) PL-427 CL2 Parts List (240 VAC) 10857 Wiring Diagram 10895 Timer/Display PCB Schematic 44416 Timer/Display PCB Layout PL-44416 Timer/Display PCB Parts List 10875 Interlock PCB Schematic 44418 Interlock PCB Layout PL-44418 Interlock PCB Parts List

These drawings were accurate at the time of publication. Changes do occur. If you have any questions regarding these drawings, please contact IEC Technical Support at (800) 843-1113.

Centra-CL2 Instruction Manual