CEM Discover Bio Installation And Operation Instructions Manual
Discover Bio
Manual Peptide Synthesizer
Installation and Operation Instructions
Contents
Introduction to the Discover Bio 2
System Requirements 3
Hardware Assembly and Setup 4
Wash Station Setup 4
Discover Setup 7
General Instrument Operation 8
Programming a New Method 8
Loading an Existing Method 8
Recommended Synthesis Parameters 9
Recommended Microwave Methods 9
Recommended Reagents for 0.1 mmol Scale 9
General Discover Bio Methods 11
Swelling the Resin 11
Performing a Cleavage 13
Using the 4 mL Vessel for Smaller Scale 15
Connecting the 4 mL Vessel 15
Maintenance Procedures 16
After Each Use 16
Monthly Maintenance 16
Semiannual Maintenance 16
Replacement of RV Frit 16
Spare Parts and Accessories 18
System 18
Accessories and Consumables 18
Performing a Deprotection 12
Performing a Coupling 12
600423
Rev. 2
August 2017
Introduction to the Discover Bio
The Discover Bio Manual Microwave Peptide Synthesizer is the newest manual microwave-enhanced peptide synthesis
system. Built on CEM’s exible Discover microwave platform, the Discover Bio allows the rapid and efcient synthesis
of peptides on scales from 0.05 to 0.5 mmol thanks to the efcient washing station and CEM’s patented circular
microwave cavity.
Discover Bio
Microwave Reactor
Product
Position
Nitrogen
Valve
Left
Knob
Discover Bio
Wash Station
Reagent
Bottles
Add Port
Right
Knob
Power
Switch
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Discover Bio™ Manual Microwave Peptide Synthesizer
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System Requirements
Bench and/or Fume Hood Space
The Discover Bio should be positioned on the bench such that access to the electrical outlets for the system is not
restricted. The Discover Bio requires the following space for system components:
Discover Bio Microwave Reactor
15” (w) x 20” (d) x 9” (h) [38 cm (w) x 51 cm (d) x 23 cm (h)]
(Depth includes 3” (8 cm) clearance behind instrument for unimpeded airow at rear fan ducts)
Discover Bio Wash Station
11” (w) x 11” (d) x 13” (h) [28 cm (w) x 28 cm (d) x 33 cm (h)]
Bottle Holder with Bottles
9.5” (w) x 9” (d) x 11” (h) [24 cm (w) x 22 cm (d) x 28 cm (h)]
Environmental Conditions
The Discover Bio is designed for indoor used only.
Temperature Range: 50 °F – 85 °F (10 °C – 29 °C)
Relative Humidity Range: 0 – 85%
Inert Gas Source
The Discover Bio requires an inert gas source (either high purity grade nitrogen or argon) capable of supplying 20
psi (20 L/min ow) within ten feet (10’)/three meters (3 m) of the rear of the instrument.
Electrical Requirements
The Discover Bio requires electrical power of 120 VAC (60 Hz, 1.7 A) (or 240 VAC [50 Hz, 1.7 A] where applicable).
Specic power requirements (120 VAC vs. 240 VAC) can be found on the nameplate afxed to the rear of the
Discover Bio Microwave Reactor and on the rear of the Discover Bio Wash Station.
Safety Information
Fume Ventilation
The Discover Bio operates as a semi-closed system, with minimal venting of any hazardous solvent fumes through
the vent line. The vent line must be vented into a proper chemical fume hood or exhaust line no longer than 10
feet (10’)/three meters (3 m) from the instrument.
In addition, adequate ventilation should be provided for preparation of reagents and solvents for use on the
system. All solvent bottles and the Waste Reservoir should be placed into proper secondary containers to minimize
the risk of exposure.
Waste Disposal
Waste produced by the Discover Bio can be hazardous. For detailed information on the safety requirements for the
chemicals used on the Discover Bio, refer to the appropriate SDS documents.
WARNING
Handle all waste under a fume hood, and wear suitable protective clothing. Dispose of all waste
in accordance with all applicable local, state, and federal heath and safety recommendations.
Discover Bio™ Manual Microwave Peptide Synthesizer
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3
Hardware Assembly and Setup
Wash Station Setup
When setting up the wash station, all tubing connections will be made on the rear of the unit. There are
eleven ports on the manifold, as well as two stainless steel ports. The proper connections for the manifold are
summarized in the table below.
Bottle Setup
Position
Number
1 Main Wash Nitrogen
2 Deprotection Nitrogen
3 Waste Vacuum
4 Main Wash Liquid
5 Deprotection Liquid
6 Waste Liquid
7 Reaction Vessel Sprayhead
8 Reaction Vessel Drain
9 Reaction Vessel Vent
10 Product Position Vacuum
11 Product Position Liquid
SS-1 Nitrogen Source
SS-2 System Vent
Tubing Connection
1. Place the three 1 L glass bottles in the bottle holder.
2. Set up the Wash Solvent bottle.
2.1. Connect a bottle cap to the 1 L bottle to be used for wash solvent. Ensure the dip tube
reaches the bottom of the bottle and that the dip tube lter is in place.
2.2. Connect the bottle tubing to the cap. The tube labeled “LIQ” should be connected to the
same port as the dip tube. The unlabeled tube should be connected to the port without a dip
tube.
2.3. Connect the other end of the bottle tubing to the rear of the Wash Station. The tube labeled
“LIQ” should be connected to position 4. The unlabeled tube should be connected to
position 1.
3. Set up the Deprotection bottle.
3.1. Connect a bottle cap to the 1 L bottle to be used for deprotection solution. Ensure the dip
tube reaches the bottom of the bottle and that the dip tube lter is in place.
3.2. Connect the bottle tubing to the cap. The tube labeled “LIQ” should be connected to the
same port as the dip tube. The unlabeled tube should be connected to the port without a dip
tube.
3.3. Connect the other end of the bottle tubing to the rear of the Wash Station. The tube labeled
“LIQ” should be connected to position 5. The unlabeled tube should be connected to position
2.
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Discover Bio™ Manual Microwave Peptide Synthesizer
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4. Set up the Waste bottle.
4.1. Connect a bottle cap to the 1 L bottle to be used for waste. Cut the dip tube to
approximately 1.5” (~4 cm) in length.
4.2. Connect the bottle tubing to the cap. The tube labeled “LIQ” should be connected to the
same port as the dip tube. The unlabeled tube should be connected to the port without a dip
tube.
4.3. Connect the other end of the bottle tubing to the rear of the Wash Station. The tube labeled
“LIQ” should be connected to position 6. The unlabeled tube should be connected to position
3.
Nitrogen and Vent Line Setup
1. Connect the nitrogen source to stainless steel port 1. Set the incoming pressure to approximately
20 psi.
2. Cut a piece of tubing (no greater than 10’ (~3 m) in length) and connect it to stainless steel port 2
using a stainless nut. This will serve as the vent line for the entire system. This tubing should be
run into a ventilated fume hood.
Product Collection Position Setup
1. Cut two pieces of tubing approximately 18” (45 cm).
2. Take one piece of tubing and, using yellow ferrules and nuts, connect one end to the left port on
the rear of the Product Collection Position, then connect the other end to position 10.
3. Take one piece of tubing and, using yellow ferrules and nuts, connect one end to the right port on
the rear of the Product Collection Position, then connect the other end to position 11.
Standard (25 mL) Vessel Setup
1. Open the reaction vessel by pressing the vessel body up into the vessel top and rotating.
2. Connect the sprayhead line.
2.1. Unscrew the sprayhead. Cut a length of tubing (~48” (120 cm)). Thread one end through the
center port on the reaction vessel top.
2.2. Put a yellow ferrule onto the end of the tubing so that the smaller end is near the end of the
tube, then press the end of the tubing into the sprayhead. Screw the sprayhead back into
the sprayhead port to secure the line.
2.3. Using a yellow ferrule and nut, connect the other end of the tubing to position 7 on the rear
of the Wash Station.
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2.4. Reconnect the reaction vessel body to the top by pressing the body up into the top and
rotating.
3. Connect the thermowell.
3.1. Place a yellow ferrule and nut on the end of the thermowell.
3.2. Insert the thermowell into one of the side ports of the reaction vessel top so that the bottom
of the thermowell is just resting on the bottom of the vessel. Secure the thermowell with
the nut.
4. Connect the reaction vessel vent line.
4.1. Cut a length of tubing (~48” (120 cm)). Using a yellow ferrule and nut, connect the tubing to
the other side port of the reaction vessel top, being careful to bend the sprayhead line out of
the way.
CAUTION
Do not overtighten the tting on the thermowell, as this can break the thermowell and damage
the ber-optic probe.
4.2. Using a yellow ferrule and nut, connect the other end of the tubing to position 9 on the rear
of the Wash Station
5. Connect the RV Drain line.
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Discover Bio™ Manual Microwave Peptide Synthesizer
600423 • Revision 2 • August 2017
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