CAROLINA BIOLOGICAL 591178, 591180 Directions For Use Manual

Wolfe Introductory Student Microscope
Models 591178 & 591180

DIRECTIONS FOR USE

Your purchase of a Wolfe Introductory Student Microscope represents an
investment. Properly used and cared for, it will provide years of uncompromising
service. While this manual assumes a basic knowledge of the compound
microscope, it highlights and reemphasizes key attributes and procedures to
ensure the greatest benefit from its design. Be sure to read and understand this
guide before and after setting up your microscope to maximize the usefulness and
ease in interpreting the information it provides. If, on any point, these features or
techniques seem unclear, the Microscope Department of Carolina Biological Supply
Company stands committed to answer your technical concerns and suggestions.

In order to properly use the microscope, an outlined series of steps follows, which
will become routine as they are repeated:

▪ Turn on the power supply with the rotating switch located on the

power cord.

▪ Rotate the 4X objective into place, perpendicular to the

microscope stage. Provision has been made that the objectives
“click” into the correct position.

▪ Using the course-focus adjustment, position the body tube so that

it is at the lower limit of its range of travel.

▪ Place a slide on the stage securely, beneath the spring-loaded

stage clips and align the specimen with the hole in the stage.

▪ Adjust the disc diaphragm so that the largest opening coincides

with the opening in the stage. This maximum aperture is generally
used at the lowest magnification.

▪ While looking through the eyepiece and using the course-focus

adjustment, slowly move the objective/body tube AWAY from the
stage/specimen until the image is clear.

▪ Rotate the 10X objective into place. While looking through the

eyepiece and using the fine-focus adjustment, clearly focus the
image, which now appears enlarged compared to that observed
through the 4X scanning objective. You will notice, increasing
magnification decreases the field-of-view or relative observable
area of the specimen.

▪ At this point, it may be beneficial to diminish the amount of light

entering the specimen. Decreasing the aperture of the disc
diaphragm will reduce glare and increase contrast in the image.
Use of the diaphragm will require experience. It is important to
select both the magnification and the degree of contrast that
renders the optimum detail in the observed specimen.

▪ Rotate the 40X objective into place and repeat the steps taken for

the initial magnification increase. The objectives of the microscope
are parfocal, meaning that the gross image will remain in focus
when changing through the objectives. It should only be necessary
to slightly sharpen the focus adjustment once the initial image is
clearly viewed at low magnification.

▪ It will be useful to repeat these procedures until they become

second nature. Your familiarity with them will add to the
usefulness of the microscope and to the ease in interpreting the

information it provides.
NOTE: If, at any or all magnifications, it is not possible to establish a clear image,
verify: that the power supply is on or the light source optimized; that all optical
surfaces are clean; that the diaphragm is properly set to allow light to pass
through the specimen; and, that the travel stop has been correctly set according to
the procedure listed further in this manual.

FEATURES

WIDEFIELD POINTER EYEPIECE: The 10X widefield eyepiece, furnished with this
microscope, contains a built-in pointer. Position the pointer by rotating the
eyepiece.
FOCUS CONTROLS (TUBE TYPE): This microscope utilizes a fixed specimen stage.
Focus is achieved through use of the course focus adjustment knob and fine focus
adjustment controls. The knobs are located on the upper portion of the
microscope arm, above the stage. You should notice vertical movement of the
body tube when focusing an image. NOTE: Between the course focus knob and
microscope stand, on one side, is a tension collar. Clockwise rotation of the collar
increases tension of the course focus adjustment.