Carolina NeuLog Oxygen User Manual
NEULOG OXYGEN SENSOR GUIDE
NeuLog oxygen sensor
NUL-205 Carolina # 369634
The oxygen sensor can be used for any science experiment or activity where oxygen
levels, dissolved or gaseous, are required. Some fields of study include Chemistry,
Physiology, Exercise Science, Biochemistry, Biology, Earth Science, Environmental
Science, etc.
Just a few of the thousands of possible experiments that can be done with the NUL205 sensor are: Monitoring of ecological systems, combustion reactions, oxygen
consumption rates, studying photosynthesis, testing chemical solutions, hydrogen
peroxide decomposition, and many more.
This sensor is practical for testing both in school laboratories and in the field and has
two modes of operation: open air mode to test gaseous oxygen concentration
(percentage of oxygen in the air), and dissolved oxygen mode for the testing of liquids
(mg/L and percentage dissolved oxygen in a liquid)
Units of measure this sensor measures are:
% oxygen concentration in air: The concentration of gaseous oxygen
present in the sample.
Mg/L: Total mass of dissolved oxygen per liter of a given liquid sample.
% oxygen in water: The percentage of dissolved oxygen present out of an
entire liquid sample.
Sensor usage and storage:
Prior to use:
1. Remove the blue rubber cap from the NeuLog oxygen probe.
2. Unscrew the tip-end membrane cap of the probe, inside you will see a metal
electrode.
3. Add 1mL of the “DO Filling Solution” directly into the tip-end which was just
unscrewed.
4. Carefully reattach the tip-end of the probe by screwing it back into the
probe.
5. Do not replace the blue rubber cap until after testing.
Storage:
Short-term (less than 24 hours):
For short term storage, fill a beaker with enough distilled water to cover the membrane
cap of the oxygen probe. With the membrane cap still attached to the probe, place it
tip side down in the distilled water. This easy storage method is ideal for short term
storage, between trials or up to 24 hours.
Long-term (more than 24 hours):
1. Unscrew the membrane cap from the oxygen probe.
2. Discard the DO filling solution from the membrane cap.
3. Rinse the cap with distilled water and gently shake to air dry.
4. Rinse and dry the exposed metal electrode with distilled water; it can be
dried with lab wipes.
5. For storage purposes: Loosely reattach the membrane cap to the oxygen
probe.
Sensor calibration:
The NeuLog oxygen sensor does not need to be calibrated prior to each use because
the prior calibration settings are stored in the sensors software. We recommend
recalibrating prior to discrete data collection such as reading the specific oxygen
content of water samples.
Open air mode:
The oxygen sensor needs to be calibrated before use. Calibration of the probe
assumes a standard atmospheric oxygen concentration of 20.9% (assuming stable
levels in the Earth‟s atmosphere at sea level). The simplest calibration is to hold the
button on the oxygen sensor down for 3-4 seconds.
NEULOG OXYGEN SENSOR GUIDE
Materials Needed:
NUL-205 oxygen sensor
1 of the following (depending on experiment type)
o USB-200 USB Module (For On-line experiments)
o BAT-200 Battery (For Off-line experiments)
o VIEW-101 Viewing Module and BAT-200 Battery (For use with
Neulog Viewer)
Procedure:
1. The oxygen Sensor needs a voltage source to properly calibrate so connect
the oxygen Sensor to:
a. For On-line experiments: USB-200 USB Module.
b. For Off-line experiments: BAT-200 Battery
c. For use with the Neulog Viewer: VIEW-101 Viewing Module
and BAT-200 Battery.
2. The sensor now needs to be calibrated:
a. For On-line experiments:
i. Connect the oxygen Sensor to the left side of a USB-
200 Module (which is connected to a computer via the
USB to mini USB cable.)
ii. Open the Neulog software
iii. Once the oxygen sensor is detected click the “Module
setup” button.
iv. Remove the blue rubber cap from the oxygen probe.
v. Hold the probe so that the sensing tip is facing the
ground.
vi. Click the “reset” button to automatically calibrate the
sensor.
b. For Off-line experiments and for use with Neulog Viewer:
i. Remove the blue rubber cap from the oxygen sensor.
ii. Press and hold the oxygen sensor‟s push button for 3
seconds while pointing the blue rubber tip towards the
ground.
3. The oxygen sensor is now calibrated for use.
Dissolved oxygen mode:
1. Connect the oxygen sensor to a PC, tablet, or smartphone and open the
appropriate NeuLog software (follow the guides below for more instruction).
2. Click the “Module setup” button on the oxygen sensor‟s icon.
3. Set the units to “%/liquid”
4. Remove the blue rubber cap from the oxygen sensor.
5. Submerge the sensor in water.
6. Saturate the water with oxygen using an air pump (manual or electric).
7. While holding the probe with the blue rubber tip facing down. Press and
hold the sensor‟s “Start/Stop” button for 3 seconds or click the “Calibrate”
button in the software (if still connected), this will calibrate the sensor to
100%.
Quick start procedure:
PC or Mac Computer
Materials needed:
NUL-205 Oxygen Sensor
USB-200 USB Module
A USB to mini USB cable (which comes with the USB-200)
Prior to initial use the Neulog oxygen sensor needs to be calibrated, please review the
“Sensor calibration” section of this document starting on page 2 for detailed
instructions. Connect the oxygen Sensor to a USB-200 Module. The USB-200
Module then connects to a computer via USB to mini USB cable. Please note that you
cannot plug the oxygen probe directly to the computer.
Resident PC software or browser base software can be downloaded for free at
www.neulog.com/dowload as well as a full software user guide. Make sure not to
download and install both types of software as they will conflict on the computer.
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