The oxygen sensor can be used for any science experiment or activity where oxygen
levels, dissolved or gaseous, are required. Some fields of study include Chemistry,
Physiology, Exercise Science, Biochemistry, Biology, Earth Science, Environmental
Science, etc.
Just a few of the thousands of possible experiments that can be done with the NUL205 sensor are: Monitoring of ecological systems, combustion reactions, oxygen
consumption rates, studying photosynthesis, testing chemical solutions, hydrogen
peroxide decomposition, and many more.
This sensor is practical for testing both in school laboratories and in the field and has
two modes of operation: open air mode to test gaseous oxygen concentration
(percentage of oxygen in the air), and dissolved oxygen mode for the testing of liquids
(mg/L and percentage dissolved oxygen in a liquid)
Units of measure this sensor measures are:
% oxygen concentration in air: The concentration of gaseous oxygen
present in the sample.
Mg/L: Total mass of dissolved oxygen per liter of a given liquid sample.
% oxygen in water: The percentage of dissolved oxygen present out of an
entire liquid sample.
Sensor usage and storage:
Prior to use:
1. Remove the blue rubber cap from the NeuLog oxygen probe.
2. Unscrew the tip-end membrane cap of the probe, inside you will see a metal
electrode.
3. Add 1mL of the “DO Filling Solution” directly into the tip-end which was just
unscrewed.
4. Carefully reattach the tip-end of the probe by screwing it back into the
probe.
5. Do not replace the blue rubber cap until after testing.
Storage:
Short-term (less than 24 hours):
For short term storage, fill a beaker with enough distilled water to cover the membrane
cap of the oxygen probe. With the membrane cap still attached to the probe, place it
tip side down in the distilled water. This easy storage method is ideal for short term
storage, between trials or up to 24 hours.
Long-term (more than 24 hours):
1. Unscrew the membrane cap from the oxygen probe.
2. Discard the DO filling solution from the membrane cap.
3. Rinse the cap with distilled water and gently shake to air dry.
4. Rinse and dry the exposed metal electrode with distilled water; it can be
dried with lab wipes.
5. For storage purposes: Loosely reattach the membrane cap to the oxygen
probe.
Sensor calibration:
The NeuLog oxygen sensor does not need to be calibrated prior to each use because
the prior calibration settings are stored in the sensors software. We recommend
recalibrating prior to discrete data collection such as reading the specific oxygen
content of water samples.
Open air mode:
The oxygen sensor needs to be calibrated before use. Calibration of the probe
assumes a standard atmospheric oxygen concentration of 20.9% (assuming stable
levels in the Earth‟s atmosphere at sea level). The simplest calibration is to hold the
button on the oxygen sensor down for 3-4 seconds.
NEULOG OXYGEN SENSOR GUIDE
Materials Needed:
NUL-205 oxygen sensor
1 of the following (depending on experiment type)
o USB-200 USB Module (For On-line experiments)
o BAT-200 Battery (For Off-line experiments)
o VIEW-101 Viewing Module and BAT-200 Battery(For use with
Neulog Viewer)
Procedure:
1. The oxygen Sensor needs a voltage source to properly calibrate so connect
the oxygen Sensor to:
a. For On-line experiments: USB-200 USB Module.
b. For Off-line experiments: BAT-200 Battery
c. For use with the Neulog Viewer: VIEW-101 Viewing Module
and BAT-200 Battery.
2. The sensor now needs to be calibrated:
a. For On-line experiments:
i. Connect the oxygen Sensor to the left side of a USB-
200 Module (which is connected to a computer via the
USB to mini USB cable.)
ii. Open the Neulog software
iii. Once the oxygen sensor is detected click the “Module
setup” button.
iv. Remove the blue rubber cap from the oxygen probe.
v. Hold the probe so that the sensing tip is facing the
ground.
vi. Click the “reset” button to automatically calibrate the
sensor.
b. For Off-line experiments and for use with Neulog Viewer:
i. Remove the blue rubber cap from the oxygen sensor.
ii. Press and hold the oxygen sensor‟s push button for 3
seconds while pointing the blue rubber tip towards the
ground.
3. The oxygen sensor is now calibrated for use.
Dissolved oxygen mode:
1. Connect the oxygen sensor to a PC, tablet, or smartphone and open the
appropriate NeuLog software (follow the guides below for more instruction).
2. Click the “Module setup” button on the oxygen sensor‟s icon.
3. Set the units to “%/liquid”
4. Remove the blue rubber cap from the oxygen sensor.
5. Submerge the sensor in water.
6. Saturate the water with oxygen using an air pump (manual or electric).
7. While holding the probe with the blue rubber tip facing down. Press and
hold the sensor‟s “Start/Stop” button for 3 seconds or click the “Calibrate”
button in the software (if still connected), this will calibrate the sensor to
100%.
Quick start procedure:
PC or Mac Computer
Materials needed:
NUL-205 Oxygen Sensor
USB-200 USB Module
A USB to mini USB cable (which comes with the USB-200)
Prior to initial use the Neulog oxygen sensor needs to be calibrated, please review the
“Sensor calibration” section of this document starting on page 2 for detailed
instructions. Connect the oxygen Sensor to a USB-200 Module. The USB-200
Module then connects to a computer via USB to mini USB cable. Please note that you
cannot plug the oxygen probe directly to the computer.
Resident PC software or browser base software can be downloaded for free at
www.neulog.com/dowload as well as a full software user guide. Make sure not to
download and install both types of software as they will conflict on the computer.
NEULOG OXYGEN SENSOR GUIDE
Procedure:
1. Install the Neulog software
2. Connect the USB-200 Module to the PC or Mac
3. Connect the oxygen Sensor to the USB-200 Module (they directly plug
together).
4. Open the Neulog software.
5. Once the oxygen Sensor logo appears on the left side of the screen the
probe has been automatically identified and you can begin experimentation.
6. If the oxygen sensor is not automatically identified then click the search
sensors icon to find the sensor.
7. Select the “On-line experiment” button; this will open a graph below.
8. Click on the “Module setup” button located on the oxygen sensor icon in the
Module window to change the oxygen probe settings if need be. (open air or
dissolved oxygen mode for example)
9. Click on the “experiment setup” button to change the experiment settings if
need be (Experiment duration for example).
10. Remove the blue rubber cap on the end of the oxygen probe.
11. The oxygen sensor will give a live reading in the box to the left of the screen
when running.
12. To run an experiment and collect data click “Run experiment”.
13. To end data collection early, click “Stop experiment”
Prior to initial use the Neulog oxygen Sensor needs to be calibrated, please review the
“Sensor Calibration” section of this document for detailed instructions. Your oxygen
sensor needs to be connected to a WIFI-201 Module. The WIFI-201 Module will
create a closed Neulog wifi network which will stream the Neulog data to a device of
your choosing. Once your device is wirelessly connected to the Neulog network you
can run experiments and collect data through a browser of your choosing.
Procedure:
1. Calibrate the oxygen sensor. (Follow the „Sensor Calibration” guide in this
document)
2. Connect the oxygen sensor directly to the left side WIFI-201 (no wires
required).
3. Connect BAT-200 to the right side of the WIFI-201
4. Although not required, we recommend plugging the BAT-200 to an outlet
using a USB to micro USB charger (such as a typical cell phone charger).
The wifi201 will run for X minutes (depending on the sensor) without being
plugged in.
5. For further WIFI-201 instructions or the WIFI-201 quick start guide please
visit www.Neulog.com/download.
6. The WIFI-201 indicator lights will flash; take no action until the LED to the
far left turns blue.
7. Take your tablet or smart phone and go to Wi-Fi settings and select the
Neulog network which matches the WIFI Module ID found on the back of the
WIFI-201 device. (NeuLogXXXX for example)
8. Give your device 1-2 minutes to connect to the WIFI-201.
9. Once the device is connected go to your browser and type the website
wifi201.com into the URL, then wait for 30-60 seconds.
10. You will see a “Control mode” icon in the browser, click on this icon.
11. The browser will then load a new screen and begin to auto detect the
sensors, this can take a few minutes.
12. If the browser does not auto detect, select “Search for Sensors”
13. Once the sensor is found you will see an icon on the left side of the screen
for the oxygen sensor. The icon will display real time data.
14. To set the oxygen Probe settings click on the “Module setup” button located
on the oxygen Sensor icon on the left side of your screen (open air or
dissolved oxygen mode for example)
15. To change the experimental settings click on the “Experiment settings”
button (Experiment duration for example).
16. Remove the blue rubber cap on the end of the oxygen probe.
17. To run an experiment and collect data select “On-Line” experiment, then
select run.
NEULOG OXYGEN SENSOR GUIDE
Operation with NeuLog viewer
Prior to initial use the Neulog oxygen sensor needs to be calibrated, please review the
“Sensor Calibration” section of this document for detailed instructions.
1. Calibrate the oxygen sensor. (Follow the „Sensor Calibration” guide in this
document)
2. Connect the oxygen sensor to the left side of the viewer.
3. Connect the BAT-200 to the right side of the viewer.
4. The VIEW-101 will auto detect the sensor and you will see it appear on the
left side of the screen.
5. Once the sensor appears it will be monitoring data in real time.
6. Remove the blue rubber cap on the end of the oxygen probe.
7. To run an experiment and collect data click the run button (little green
person)
Off-line experiments
(Off-line experiments are for when you do not have a sensor connected directly to a
computer, tablet, smartphone, or NeuLog viewer).
Materials needed:
NUL-205 Oxygen Sensor
BAT-200 Battery
Materials needed to configure your offline experiment:
USB-200 USB Module or WIFI-201 WIFI module or VIEW-101 Viewing Module
A USB to mini USB cable (which comes with the USB-200)
Procedure:
1. Calibrate the oxygen sensor. (Follow the „Sensor Calibration” guide in this
document.
2. Connect oxygen sensor directly to the left side of the charged BAT-200
Module
3. Remove the blue rubber cap on the end of the oxygen probe.
4. When ready to collect data press the “Start/Stop” button on the oxygen
Sensor, a red light will turn on for the duration of the experiment.
5. To change the experiment settings first connect to your chosen device (PC,
Mac, tablet, smart device) and select the “Module setup” button. (For more
information on how to change experiment settings view quick start
procedure section for your chosen device in this document.)
6. After the experiment has concluded (when the “Start/Stop” button is pressed
again or the data collection period ends) connect the sensor to your chosen
device. (full instructions in each device‟s quick start procedure section)
7. Open Neulog software.
8. Click the “Off-line experiment” button.
9. Click the “Load data from sensors” button.
10. Select which experimental data to upload. (5 experimental runs can be
stored at one time on the NUL-205 oxygen Sensor)
Restoring sensor’s factory default settings:
Procedure:
1. Connect the USB-200 to a PC, Mac, tablet, or smart device.
2. Connect the oxygen sensor to a USB-200 module (they directly plug
together).
6. If prompted to clear the graph, click either the “Off-line experiment” or “On-line experiment” (whichever you are using).
7. Click “Clear experiment results”.
8. Resume from step 4.
NEULOG OXYGEN SENSOR GUIDE
Sensor specifications
In air
Dissolved
(%)
Dissolved
(mg/L)
Range and
operation
modes
0 to 25%
0 to 125%
0.01 mg/L
ADC resolution
12 bit
Resolution
0.01%
0.01%
0.01 mg/L
Max sample
rate (S/sec)
100
Included with sensor:
NeuLog NUL-205 Oxygen Sensor Guide (this document).
Neulog oxygen sensor probe which is attached to the sensor body by a durable
rubber-coated wire.
30mL oxygen sensor filling solution.
Replacement membrane cap for the oxygen sensor.
Sensor features:
Fully digital data.
Rugged plastic ergonomic case.
Push button switch for Start/Stop experiments in off line mode.
LED indicator of experiment status (blinks while collecting data).
Attached probe cased in durable plastic.
Note: Neulog products are intended for educational use.
Videos and experiment examples:
Videos, literature and other probes can be found at www.neulog.com
http://www.neulog.com/Products/oxygenloggerSensor.aspx
Respiration -
http://www.neulog.com/Portals/3/Users/Experiment B-3 Respiration Ver 3.2.5.pdf
http://www.neulog.com/Portals/3/Users/Experiment C-6 Gas Solubility Ver
3.2.4.pdf
Technical background:
The philosophy behind Neulog‟s plug and play technology is based on each sensor‟s
ability to store its own data due to an internal flash memory chip and micro-controller
in each plastic Neulog body. This technology allows the sensor to collect and then
store the digital data in the correct scientific units (oC, oF, Lux, %, ppm, for example).
The sensor needs to be calibrated prior to initial use, please review the “Sensor
Calibration” guide in the beginning of this document for detailed instructions.
An electrochemical cell, containing a lead anode and a gold cathode which are
submerged in electrolytes, reacts with oxygen molecules as they enter the cell. The
oxygen is reduced at the gold cathode (meaning it donates spare electrons) to create
a measurable electrical current. The strength of the current is directly proportional to
the amount of oxygen being reduced. Based on the current strength, the oxygen
concentration can be easily calculated
Maintenance and storage:
Never submerge the Neulog sensor‟s plastic body in any liquid.
Do not allow liquid into the Neulog plastic body.
After using the probe wipe off all excess material, liquid or residue from the
oxygen probe body.
Store in a box at room temperature out of direct sunlight.
Do not put the plastic case, or cord of the oxygen probe in an acids or
bases.
Store the oxygen sensor‟s probe upright with the blue rubber tip pointing
downward.
Do not use the sensor in acid or alkaline solutions with a molarity greater
than 1.0.
NEULOG OXYGEN SENSOR GUIDE
Warranty:
We promise to deliver our sensor free of defects in materials and workmanship for a
period of 3 years from the date of purchase. Our warranty does not cover damage of
the product caused by improper use, abuse, or incorrect storage. Sensors with a shelf
life such as ion selective proves have a warranty of 1 year. Should you need to act
upon the warranty please contact your distributor. Your sensor will be repaired or
replaced.
Thank you for using NeuLog!
Flexible, simple, fast, forward thinking.
W: www.neulog.com
E: info@neulog.com
A: 850 St Paul Street, Suite 15, Rochester, NY 14605
P: 1.866.553.8536
V04302013
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