BRIGHT M3500 MICROTOME Operating, Maintenance And Spare Parts Manual

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M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

Operating, Maintenance and

Spare Parts Manual for the

Bright

M3500 MICROTOME

Serial Number: ………………………

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M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

CONTENTS

OPERATING, MAINTENANCE AND .............................................................. 1

SPARE PARTS MANUAL FOR THE .............................................................. 1

BRIGHT ........................................................................................................... 1

M3500 MICROTOME ....................................................................................... 1

Serial Number: ……………………… ................................................................................................. 1

CONTENTS ........................................................................................... 2,3 & 4

SAFETY AND GENERAL INFORMATION ..................................................... 6

Consumer Protection .......................................................................................................................... 6

ELECTRICAL .................................................................................................................................... 6

MICROTOME KNIVES/BLADES ...................................................................................................... 6

OPERATION .......................................................................................................................................... 7

ACCESSORIES ...................................................................................................................................... 7

PRODUCT SAFETY SUGGESTIONS ................................................................................................ 7

DECONTAMINATION CERTIFICATES .......................................................................................... 7

WARRANTY .......................................................................................................................................... 8

1. INTRODUCTION ....................................................................................... 9

1.1 Receipt and Unpacking ...................................................................................................... 9

1.1.1 Receipt ............................................................................................................................. 9

1.1.2 Unpacking ....................................................................................................................... 9

1.1.3 Lifting .............................................................................................................................. 9

1.2 ASSEMBLY AND INSTALLATION ........................................................ 9

1.2 Electrical connections ......................................................................................................... 9

1.2.1 Electrical requirements .................................................................................................. 9

1.2.2 Electrical Safety .............................................................................................................. 9

1.2.3 Switching on .................................................................................................................... 9

2. OPERATING INSTRUCTIONS .................................................................. 9

2.1 Front Control Panel .......................................................................................................... 10

2.2 REAR FUSE PANEL ............................................................................ 10

2.1.1 Mains Switch ................................................................................................................. 10

2.1.2 Fuse ................................................................................................................................ 10

2.3 PRE-USE CHECKS .............................................................................. 11

2.4 MICROTOME CONTROLS ................................................................... 11

2.4.1 Specimen cassette .......................................................................................................... 11

2.4.2 Knife Holder .................................................................................................................. 11

2.4.3 Disposable Blades ......................................................................................................... 11

2.4.4 Rewind Control (Manual) ............................................................................................ 12

2.4.5 Section Thickness Control ........................................................................................... 12

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M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

2.5 MOTORISED ADVANCE/REWIND (WHERE FITTED) ........................ 12

2.5.1 General .......................................................................................................................... 12

2.5.2 Motorised Specimen Cassette Rewind ........................................................................ 12

2.5.3 Motorised Specimen Cassette Advance ...................................................................... 12

2.6 OTHER FEATURES ............................................................................. 12

2.6.1 Debris Trays .................................................................................................................. 12

2.6.2 Visor ............................................................................................................................... 13

2.6.3 Hand wheel .................................................................................................................... 13

2.7 CUTTING AND COLLECTING SECTIONS .......................................... 13

2.7.1 Set Up ............................................................................................................................. 13

2.7.2 Wax Cassette Set Up ..................................................................................................... 13

2.7.3 Trimming ....................................................................................................................... 13

2.7.4 Section Cutting .............................................................................................................. 13

3. GENERAL MAINTENANCE .................................................................... 14

3.1 Daily Care .......................................................................................................................... 14

3.2 Microtome Knives and Blades ......................................................................................... 14

3.2.1 Conventional Knives ..................................................................................................... 15

3.2.2 Disposable Blades ......................................................................................................... 15

3.3 SERVICING AND REPAIRS ................................................................. 15

3.3.1 Routine Servicing .......................................................................................................... 15

3.3.2 Repairs ........................................................................................................................... 15

3.4 DECONTAMINATION .......................................................................... 16

3.4.1 Disinfectant ................................................................................................................... 16

4. SECTIONING ........................................................................................... 16

A PROBLEM SOLVING QUIDE ....................................................................................................... 16

4.1 CHECK LIST ........................................................................................ 16

4.1.1 Specimen ........................................................................................................................ 16

4.1.2 Knife............................................................................................................................... 16

4.1.3 General Factors ............................................................................................................. 16

4.2 THICK / THIN SECTIONS .................................................................... 17

INTERMITTENT FAILURE TO CUT .............................................................................................. 17

4.3 SECTIONS CRUMBLING OR NOT FORMING .................................... 17

4.4 EXCESSIVE COMPRESSION OF SECTIONS ..................................... 17

4.5 UNEVEN THICKNESS ACROSS SECTION ........................................ 17

4.6 VERTICAL SCORE MARKS ON SECTIONS ....................................... 17

4.7 SECTIONS FAIL TO FLATTEN AND PASS DOWN KNIFE ................ 18

5.0 MISCELLANEOUS SERVICING INSTRUCTIONS .............................. 18

5.1 Advance Pawl Replacement ............................................................................................. 18

5.2 Pawl Release Lever (Advance)......................................................................................... 18

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5.3 Calibration ........................................................................................................................ 18

9. SPECIFICATIONS ..................................................................................... 21

Accessories ........................................................................................................................................ 22

MATERIAL SAFETY DATA SHEET ............................................................. 23

Name: CRYO-M-BED .............................................................................................................. 23

MATERIAL SAFETY DATA SHEET ............................................................. 24

Name: CRYOSPRAY ............................................................................................................... 24

MATERIAL SAFETY DATA SHEET ............................................................. 25

Name: Low temperature Grease ............................................................................................. 25

Knives / Blades .................................................................................................................................. 26

DECONTAMINATION CERTIFICATE ........................................................... 27

DECONTAMINATION PROCEDURES ......................................................... 28

Microtomes ............................................................................................................................................ 28

Microtome Knives ................................................................................................................................ 28

QUALITY SURVEY REPORT........................................................................ 29

Annexe:

Drawing 212-040 M3500 Microtome Assembly 1 of 3 30, 31 & 32 Part List 212-040-PL 33 & 34

Drawing 51937 Cutting Arm Assembly 35 Part List 51937-PL 36

Drawing 212-041 Microtome Motor Rewind Sub-Assembly 37 Part List 212-041-PL 38

Drawing 212-046 CQRS Non-Orientating Assembly 39 Part List 212-046-PL 40

Drawing 212-047 CQRS Orientating Head Assembly M3500 41 Part List 212-047-PL 42

Drawing 212-051 Pawl Release Assembly 43 Part List 212-051-PL 44

Drawing 212-054 M3500 Knife Block Retract Model Sub-Assembly 45 Part List 212-054-PL 46 & 47

Drawing 212-061 M3500 Input Drive Shaft (Hand wheel) Assembly 48 Part List 212-061-PL 49

Wiring Diagram 50

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Power Indicator Section Counter Reset

Section Counter

Visor

Thickness Selector Knob

Locking L

eve

Rear

Cove

Cutting Handwheel

RH Advance/Rewind

Switc

50230 Standard Knif

RH Knife

Guard

Knife Guard H

eight

Knob

Debris Tray

Knife Clamps

Cassette Release L

eve

Manual A

dv/Rwd

Knob

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SAFETY AND GENERAL INFORMATION

Consumer Protection

The Consumer Protection Act 1987 Part 1. refers to Product Liability. This legislation was issued as a direct result of an EC Directive to all member states and has been in force with effect from 1 March 1988.

The Bright Instrument Company Limited, ever mindful of the need to ensure that their products are not subject to misuse and/or incorrect handling, have made it their aim to communicate any possible dangers to their customers.

Whilst the Bright Instrument Company Limited markets products manufactured to the highest safety standards, it is in the interest of the purchaser that he is aware of the resultant dangers of misuse and/or incorrect handling of these products.

Your attention is therefore drawn to the following precautions:

ELECTRICAL

a. Warning - A warning notice is fixed to the instrument (where applicable) stating that

it should be disconnected from the mains supply before removing the panels. This warning should be strictly observed.

b. Fuses – Fuse ratings are clearly indicated on all fuse panels adjacent to the fuse

holder. If and when replacement is necessary, the correct fuse rating and type must be adhered to.

c. Earthing (Grounding) – A protective earth terminal is fitted and must be used in all

two-wire installations.

MICROTOME KNIVES/BLADES

The microtome knife/blade can be a hazard in the laboratory. Personnel should be made aware of the dangers and observe the following warnings:

a. DO NOT leave the microtome unattended with an exposed knife/blade in position.

Remove the knife/blade, or cover with the guards or visor provided.

b. DO NOT leave unboxed knives/blades lying around. Place knives/blades that are not

in use in their boxes or packets.

c. DO NOT carry knives/blades unless secure in the box or packet provided.

d. DO NOT clean the knife/blade along its length. Wipe the knife/blade from the back

edge to the cutting edge.

e. REMEMBER that even used knives and blades are dangerous. The are still sharp

and may have been used to cut potentially infectious specimens.

f. DISPOSE of used knives and blades with the same care as other sharp objects. On

no account should used knives or blades be placed in waste bins.

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OPERATION

When placing specimen cassettes in the microtome, when orientating, manipulating or in any way placing fingers in a position above the knife/blade edge – ensure that the hand wheel is locked and knife guards are in position.

ACCESSORIES

The instrument has the following accessories as standard:

Low temperature grease: (please refer to the material safety data information, towards the back of this instructions manual, for further information).

Long series ball driver tool, to remove / fit rear cover 2 pin tool, to remove / fit the hand wheel and visor Allen keys, fuse and brush

PRODUCT SAFETY SUGGESTIONS

All Bright Instrument Company Limited personnel are encouraged to make suggestions regarding product safety. We also welcome such suggestions from our customers. They may be submitted by completing the appropriate (Safety) section of the Quality Survey Record Form supplied with all Bright instruments, or alternatively, by letter, telephone, fax or email. All communications should be direct to our Quality Assurance Department and will be acknowledged.

DECONTAMINATION CERTIFICATES

IMPORTANT

If the instrument or any part of it is to be returned to Bright Instrument Company Limited, a decontamination certificate must accompany it, and please note the following:

1. If the instrument or any part of it has been exposed to or been in contact with potential pathogenic or radioactive material, it is essential that it be decontaminated.

2. Set procedures are laid down by the Health and Safety Executive for decontamination.

The “Howie code” and the “Code of Practise for the Prevention of Infection in Clinical Laboratories and Post-mortem Rooms”, ISBN 0 11 320464 7) explain the correct procedures to be used. For the avoidance of doubt, we ask that a completed decontamination certificate should accompany instruments or parts returned to us. A copy of this can be found at the back of this instruction manual and we suggest you use a photocopy of this when returning parts. Alternatively we would be pleased to either post or fax you another copy should you require.

3. Should the instrument or any part of it be received in a condition that Bright Instrument Company Limited, consider to be a potential biological hazard, the instrument or part will be returned unrepaired at the expense of the customer.

4. Customer’s declarations must indicate that the package contains ‘British Returned Goods’. Failure to do so will involve customs duty payable by us, which will be invoiced to the sender.

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M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

WARRANTY

The Warranty relating to the instrument is outlined in our Terms and Conditions of Sale paragraph 6.

i) The Seller‘s products are carefully inspected and submitted to its standard tests.

II) The Seller warrants all its products to be free from defects in workmanship and

materials under normal conditions of use and service provided always:

a. that if any of the goods so manufactured is alleged to be defective in workmanship

and material and is returned carriage paid, and protected against damage in transit to the Seller’s works at Huntingdon, England within 12 months from the date of despatch and if after examination by the Seller that goods or part of them are found to be so defective then the Seller will repair or replace them free of charge and will return them to the Buyer , carriage paid.

b. where any part of the goods manufactured by the Seller is repaired or replaced under

the terms of the foregoing warranty, such warranty shall thereafter be limited to a period of six months from the date when the goods shall have been re-delivered to the Buyer.

c. this warranty does not apply to any defects caused by wear and tear, incorrect

installation abnormal conditions of working, accident, misuse or neglect.

d. that save as in this clause herein before expressed, the Seller shall not be under any

liability for negligence or otherwise in respect of defects in goods delivered or for any injury, damage or loss resulting from such defects and the Seller’s liability under this clause shall be in lieu of any warranty or condition implied by law as to the quality or fitness for any particular purpose of such goods.

e. this warranty is expressly in lieu of all other warranties, guarantees or liabilities

expressed or implied by any of the Seller’s Representatives or Agents.

Please see our separate Product Warranty sheet for deliveries to the mainland UK.

WARNING

Before proceeding to Operating Instructions, ensure you are familiar with the contents of the pages marked ‘Safety Information’. This instrument must only be used by competent persons.

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M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

1. INTRODUCTION

1.1 Receipt and Unpacking

This instrument received a final test and inspection prior to despatch from the factory. The following instructions are given for the re-assembly of the instrument, adjustments and its correct use. If the instrument is received before preparations for installation are completed, it should be stored in a clean, dry place and not exposed to dirty or damp conditions.

1.1.1 Receipt

Immediately upon receipt of the instrument, make a careful examination for evidence of damage encountered in transit. If any damage is found or suspected, notify both the carrier and Bright Instrument Company Limited.

1.1.2 Unpacking

All packing must be carefully removed and parts checked against the enclosed packing list. If any damage or discrepancy is noted, please inform our agent/distributor, or Bright Instrument Company Limited, direct, immediately.

1.1.3 Lifting

The instrument has been designed with two main lifting points as hand holds at the front and rear of the instrument. The instrument weight less than 25Kgs, but care should be taken when lifting by following manual handling guide lines. If in doubt contact your Health & Safety Officer.

1.2 ASSEMBLY AND INSTALLATION

The microtome is delivered virtually ready for use with only the accessories required for use in need of fitting.

1.2 Electrical connections

1.2.1 Electrical requirements

The supply cord of the instrument should be connected to any ordinary electrical outlet (max 13 amps). Check the voltage stamped on the nameplate, located on the underside of the instrument, with your supply:

The connections are:

Brown - Positive [live]

Blue - Negative [neutral]

Yellow/Green - Earth [ground]

1.2.2 Electrical Safety

Where earth connections are taken through connectors, then the connector must be rated to take the maximum fault current. The machine should be disconnected before such connectors are separated for servicing purposes.

1.2.3 Switching on

Switch on by using the Mains Power switch located on the rear fuse panel. This is applicable only to Motorised Advance/Rewind models.

For instruments with a section counter only, this is operated by 2 AA batteries located on the rear panel at the bottom of the plinth.

2. OPERATING INSTRUCTIONS

On receipt of your new Bright instrument, please refer to section 1.1 (Receipt and Unpacking) and section 1.2 (Assembly and Installation).

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As part of its policy of continual improvement, Bright Instrument Company Limited, reserves the right to incorporate changes, or make additions to, without prior notice. There may, therefore, be minor details differences between the information in this manual and your instrument. These differences will not affect the safety and use of the instrument.

2.1 Front Control Panel

This panel houses the section counter, the re-set button to zero the counter and a Mains Power LED for indicating purposes.

2.2 REAR FUSE PANEL

2.1.1 Mains Switch

The mains power switch provides power to the motorised advance/rewind control only.

2.1.2 Fuse

The single fuse is for the Main Power only.

Main - All circuits

If the fuse blows, replace it with a new one of exactly the same rating. In the event of repeated fuse blowing, consult a qualified electrical engineer.

On NO account must the fuse be replaced with anything other than a correctly rated substitute.

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2.3 PRE-USE CHECKS

With the instrument on the work surface open the visor and ensure that the cutting arm will strike no objects. With the section thickness dial set at zero and using the mechanical advance/rewind knob, advance the lead screw body from the cutting arm.

Unlock the hand wheel and rotate in direction of arrow. Ensure hand wheel runs smoothly and the section counter is operating.

For models with Motorised Advance/Rewind, ensure you have the correct power supply and fuse rating before plugging in and applying power.

With power applied ensure the section thickness dial is set at zero and the cutting arm in the top position, repeat operation above using either of the Advance/Rewind switches located on the left hand and right hand side of the Plinth.

2.4 MICROTOME CONTROLS

2.4.1 Specimen cassette

IMPORTANT: Ensure knife guards are in a central position if a knife is installed.

To install a specimen cassette, pull the release lever forward with your finger. Place the cassette in position and release the lever.

To orientate (if option ordered) the specimen cassette, move the lever to the left to release the clamp, then move the cassette to the desired position. Re-lock the lever.

2.4.2 Knife Holder

To install a knife or disposable blade holder, loosen the clamp screws, slide the knife guards to the centre, and slide knife through from the left.

Before re-tightening clamp screws, set knife tilt angle (against the degree scale graduated 025º). The correct setting of the knife tilt angle is essential for obtaining good results. With a Bright 50230 knife, set an angle of 15º. With a Bright Lever Release Feather Blade Holder cat no. 57903 the angle should be set to 5º.

2.4.3 Disposable Blades

The M3500 can be fitted with the Bright ‘Magnacut’ disposable blade system (not recommended on Wax) or with a suitable alternative holder for ‘Feather’ blades, some experimentation may be required for optimum results. Generally a knife tilt angle of 5º is a good starting point.

Push towards centre Push towards centre

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IMPORTANT: Knife guards must be used whenever a knife or disposable blades are in position. Take care to guard the central portion of the knife whenever sections are not being cut.

2.4.4 Rewind Control (Manual)

The rewind control is used to reset the microtome advance system and for trimming specimens.

NB: Lock the hand wheel in the three o’clock position and section thickness to zero – this ensures the advance mechanism is disengaged.

2.4.5 Section Thickness Control

Select section thickness required by turning the knob to the appropriate position. There are increments of 2µm for 2 - 30µm (or if option ordered for 1µm for 1 -15µm lead screws).

2.5 MOTORISED ADVANCE/REWIND (where fitted)

2.5.1 General

The motor used on this machine is a Pulse DC Stepper Motor. As with all stepped motors, there is an inherent noise which you may find distracting at first. This minimal noise is caused by the ’pulsing’ of the motor which is particularly noticeable when the motor is no longer ‘driving’. It in no way affects the operation of the microtome, or has any other adverse effects.

The motor is operated by using either of the two switches located on the left and right hand sides of the Plinth.

The Motorised Advance/Rewind should only be operated with the cutting head below the 3 o’clock position and the section thickness control set to 0.

2.5.2 Motorised Specimen Cassette Rewind

- to rewind the cassette follow instructions above

- for small rewind increments, press and quickly release the switch.

2.5.3 Motorised Specimen Cassette Advance

- set specimen thickness control to 0µm

- rewind specimen as required

- with microtome knife set at the correct angle with the specimen in position, use the

switch to ‘advance’ until a minimum clearance has been achieved

- begin trimming by pressing and releasing ‘advance’ switch whilst rotating hand wheel

2.6 OTHER FEATURES

2.6.1 Debris Trays

Two debris trays are provided – one is the internal part of the microtome that surrounds the knife block. The second tray is internal and sits under the Main Cutting Arm with the sole purpose of collecting any stray debris that may have found its way past the Debris Blind. To access the second tray, you must remove the External Debris Tray.

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The debris trays can easily be lifted out for emptying and cleaning, but first brush most of the debris into the deeper sections of the trays.

Do not expose the trays to temperatures in excess of 60ºC (see section 3.4)

2.6.2 Visor

The visor is a plastic translucent moulding which can be usd as a safety feature by closing when the unit is not in use to prevent someone inadvertently touching the settings, blade etc.

2.6.3 Hand wheel

The operating hand wheel on the right side of the Microtome can be locked with the thumb lever. There are ten locking positions including tip and bottom. The hand wheel should always be turned clockwise.

2.7 CUTTING AND COLLECTING SECTIONS

We recommend the following cutting angles when using:

a. leaver release Feather blade - 5º

b. solid knife [Bright 50230] - 15º

2.7.1 Set Up

- fit a sharp knife in position, ensuring knife guards are in central position

- check hand wheel is locked with specimen cassette in top position

- move knife guards to the side

2.7.2 Wax Cassette Set Up

- place the wax cassette in the quick release holder with the angled end pointing down

- set the section thickness to zero

- slowly turn the operating handle to lower the cassette in proximity to the blade or

knife

- wind the advance/rewind control to take the wax block so that it just touches the back

of the blade or knife. At the same time move the operating hand wheel with a gentle backwards and forwards motion so that the cassette goes gently up and down.

2.7.3 Trimming

- use the advance/rewind control to carefully increment the wax cassette towards the

blade or knife while fully turning the operating hand wheel. Avoid taking too large ‘bites’ at the wax.

- alternatively, if Motorised advance/rewind is fitted, the wax cassette can be

incremented towards the blade or knife by briefly pressing one of the advance/rewind switches.

- Another method is simply to set the section thickness control to 14µm and turn the

operating hand wheel until the specimen is suitably exposed.

Take care while trimming , particularly with small specimens or hard/tough tissues.

2.7.4 Section Cutting

- after trimming, set the section thickness control to the desired position and turn the

operating hand wheel slowly

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- once sections are seen to be cut as required proceed to cut sections

NB: For best results the wax cassettes should be cooled to their optimum sectioning temperature

Keep the knife clear of debris by brushing upwards with the knife cleaning brush. NEVER brush along the knife, always brush upwards.

3. GENERAL MAINTENANCE

3.1 Daily Care

Routine daily care consists of removing sectioning debris from the working area, brushing debris from the knife and cleaning as appropriate.

- first remove the microtome knife/blade holder and clean it (see section 3.4)

- remove the debris trays and clean

- wipe covers and remove excess debris as required

- wipe visor if required

Note that the microtome requires no other routine maintenance.

3.2 Microtome Knives and Blades

Great care must be exercised when handling knives and or disposable blades.

- knives/blades must be stored in their boxes when not in use

- knives /blades fitted to the microtome must be properly guarded

- particular care must be taken during cleaning and knife sharpening

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3.2.1 Conventional Knives

Conventional microtome knives are usually made from carbon steel and will corrode in moist conditions

Bright Instrument Company Limited offer a knife sharpening service, please contact Sales for further information.

3.2.2 Disposable Blades

Take particular care to clean the contact surfaces of disposable blade holders. A build up of debris can prevent the blade from seating properly and cuases instability during section cutting.

3.3 SERVICING AND REPAIRS

3.3.1 Routine Servicing

With the exception of items mentioned in section 3.1, there is no requirement for periodical checks.

3.3.2 Repairs

In the event of a breakdown, a qualified person should be called. For electrical and mechanical problems contact either your local agent/distributor or bright Instrument Company Limited. Please provide the following information:

Model Serial Number Date of Installation Nature of Fault

The following tasks can be carried out by competent personnel:

- changing fuses

1. switch off power

2. unscrew fuse holder

3. replace fuse with correct fuse rating

4. screw back in fuse holder

If the microtome or any part of it is returned to the agent/distributor or manufacturer, it is important to observe the precautions in section 3.4 to minimise the risk of infection.

NB: A completed decontamination certificate must be attached to the exterior of the microtome. Work on the instrument will not proceed until satisfactory notification of decontamination has been received.

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3.4 DECONTAMINATION

It is the responsibility of the user to ensure that a decontamination procedure is employed which is appropriate to the nature of the work carried out.

Hypochlorite (bleach) solutions are corrosive to many metals and should be avoided.

Do not expose the debris trays to temperatures in excess of 60ºC.

3.4.1 Disinfectant

There are many wide-spectrum biocidal disinfectants on the market that are used in microbiological and clinical departments. These may be used to disinfect the microtome.

- Make up the solution according to the manufacturers instructions

- Wipe around the microtome with a cloth or paper towel wetted with the solution.

Ensure all debris is collected and all surfaces have ample contact with the solution.

- Wipe over again with clean water.

- Alcohol may be used as an alternative.

- Debris screens are fitted to the microtome, which stop section trimmings etc from

entering, so the interior of the microtome should not require decontamination but please check the internal debris tray.

4. SECTIONING

A PROBLEM SOLVING QUIDE

Cutting sections requires skill and practice, and some tissues are particularly difficult. In all cases the quality of the result depends on a number of factors.

The notes which follow deal with the more commonly encountered difficulties, and suggest a systematic approach to problem solving.

4.1 CHECK LIST

First check all the basic factors:

4.1.1 Specimen

- has it become loose on the cassette ?

- is the cassette tightly clamped ?

- is the orientation lever locked ? [if applicable]

- has the specimen dehydrated ?

4.1.2 Knife

- has it become dull ?

- is it nicked or chipped ?

- is the knife tilt angle incorrect ?

- is the knife loose ?

- is there a build-up of frost or debris on the knife ?

4.1.3 General Factors

- is the cutting motion too fast/slow/uneven ?

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- has the microtome reached the end of its travel ?

- is the section thickness setting appropriate ?

4.2 THICK / THIN SECTIONS

INTERMITTENT FAILURE TO CUT

- knife/blade not sharp enough

- knife/blade tilt angle too high/low

- clamping screws too loose

- cassette loose on holder

4.3 SECTIONS CRUMBLING OR NOT FORMING

- specimen dehydrated

- knife/blade not sharp enough

- cutting motion too fast or uneven

- knife/blade tilt angle too high/low

4.4 EXCESSIVE COMPRESSION OF SECTIONS

- knife/blade not sharp enough

- knife/blade tilt angle too high

- knife/blade surfaces debris built up

4.5 UNEVEN THICKNESS ACROSS SECTION

- clamping screws too loose

- cassette loose in holder

- knife/blade not sharp enough

- knife/blade edge too thin

- cutting motion uneven

4.6 VERTICAL SCORE MARKS ON SECTIONS

- knife/blade edge nicked or chipped

- knife/blade faces have build up of debris or frost

- tissue contains hard, fine particles (eg: bone fragments)

NB: When removing debris always brush up the knife, NEVER down or along

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4.7 SECTIONS FAIL TO FLATTEN AND PASS DOWN KNIFE

- knife/blade surface has build up debris

5.0 MISCELLANEOUS SERVICING INSTRUCTIONS

5.1 Advance Pawl Replacement

a. Remove rear cover by removing the 4 securing screws (rear 2 screws under visor

stoppers) and raise and support Drive Arm.

b. Using a thin bladed screwdriver, lever the spring from the hole in the bottom of the

pawl. Unscrew the slotted screw and remove slowly, being careful not to lose the spacer.

c. Position the pawl so the spring is located in the hole at bottom of the pawl. Put the

screw into the pawl so that the screw comes into the middle of the spring levers, slide

the spacer onto the screw and attach to the pawl arm assembly, tighten the screw.

d. Carry out a functional check and if satisfactory replace rear cover.

5.2 Pawl Release Lever (Advance)

This lever is attached to the underside of the Long debris Blind Roller Support. 0µm and the main arm is in the top position, the short lever holds the pawl away from the ratchet wheel. This will leave a sufficient gap as to let the ratchet wheel rotate freely. Adjust the short lever as required by moving the lever towards or away from the ratchet wheel at its pivot point.

5.3 Calibration

Calibration is set by selecting 2µm. One click from the pawl and ratchet wheel should be heard.

Check by cutting wax sections so you can actually see the thickness change through micron settings of 2, 4 & 6µm and that it is not cutting at zero thickness.

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6 Exploded Diagram

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7. Internal Construction - Front

8. Internal Construction – Rear

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9. SPECIFICATIONS

M3500 MICROTOME

Movement

●

Radial, flat cutting with retraction, manual hand wheel operation

Cutting Stroke

●

57mm

Section thickness range

●

2µm to 30µm in 2µm increments (standard)

●

1µm to 15µm in 1µm increments (Special)

Total feed range

●

25mm at 0º knife tilt angle

Specimen holders

●

Quick release non-orientating holder (standard)

Other Notes:

●

Standard Knife

●

Disposable blades

●

Lever release Feather blade holder available on request

●

Manual & mortised advance/rewind facility

●

Height adjustable knife guards

●

Safety visor

DIMENSIONS

General

●

Overall height - 300mm

●

Overall width - 450mm

●

Overall depth - 440mm

Weight

●

<24Kgs

ELECTRICAL

Voltage

●

220-240v, 110-115v, 100v

●

50/60Hz

●

13amp

Batteries

●

2 x AA (for section counter)

Fuse

●

Motorised advance/rewind stepper

Page 22

M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

Accessories

Code Description

212-048 Standard vice clamp, can be fitted instead of quick

release cassette holder at no extra cost

212-049 Standard vice clamp with orientation

212-046 Cassette quick release holder

212-047 Cassette quick release holder with orientation

50230 Standard knife with box, C

profile

50724 Knife sharpening back for 50230

52671 Knife sharpening kit

57612 Coarse abrasive 6 grms net

57611 Fine abrasive 6grms net

57610 Honing compound 6grms net

50239 Clock oil 6grms net

57903 Lever release Feather blade holder

50241 Pack of 50 feather blades

138404-16 Spare fuses 1

amp(T)

52658 Vertical vice clamp

52632 Vertical vice clamp 45°

57713-1 Bright Cryospray 134, 300ml aerosol can

57713 Bright Cryospray 134, 300ml aerosol can, carton of 12 cans

57355 Low temperature grease, 5gms net

57344 Knife cleaning brush

Page 23

M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

MATERIAL SAFETY DATA SHEET

Name: CRYO-M-BED

Part Number: 53581

Product Information

By: Bright Instrument Company Limited

Address: St Margaret’s Way, Huntingdon, Cambs, PE29 6EU, England

Telephone: 01480 454 528 / 451 499 / 451 980

Emergency: 999

Fax: 01480 456 031

Email: [email protected]

Trade/Type: EMBEDDING COMPOUND

Container: Disposable plastic bottles

Uses: Embedding compound for frozen tissue

specimens

Data Sheet: 3

Description: Colourless viscous liquid

Information on Ingredients Physical and Chemical Properties

Blend of polyviol alcohol 217, thymol and water

Colourless viscous liquid

Hazards Identification: Stability and Reactivity

Skin: Can cause skin irritation May react with oxidising materials Respiratory: May cause difficulty in breathing if

exposed to very high concentration Ingest: May be harmful by ingestion Eyes: Eye irritation

First Aid procedures Toxicoloigical Information

Skin: Wash thoroughly, with soap and

water

No harmful effects if handled correctly. May give off toxic

fumes in the case of fire Respiratory: Move to fresh air Ingest: Rinse mouth out with water, in

sever cases seek medical attention

Eyes: Flush copiously for at least 15

minutes

Fire Fighting Measures Ecological Information Hazards: May cause toxic fumes Degradable, miscible in all proportions Equipment: Water spray, foam, dry powder,

Co2

Accidental Release Measures Disposal Considerations

Spill: Absorb on an inert absorbent, bag

and arrange disposal. Wash area in water and detergent

Waste: Bag and dispose of in accordance

with local authority requirements

Handling and Storage Transport Information

Special Requirements:

NONE No restrictions

Exposure Controls Regulatory Information

OES: Not assigned (long term, 8 hour

TWA)

NONE

Skin: Avoid contact Respiratory: Avoid very high concentrations Ingest: Do not eat, drink or smoke Eyes: Goggles should be worn Additional Information/Comments: Information given is, to the best of the Company’s knowledge and belief, accurate and reliable. However, no warranty, guarantee or representation is made to it’s accuracy, reliability of completeness.

Issue 6 /June 2000 - Last reviewed: Sept 2010 / Next review: Sept 2012

Page 24

M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

MATERIAL SAFETY DATA SHEET

Name: CRYOSPRAY

Part Number: 57713

Product Information

By: Bright Instrument Company Limited

Address: St Margaret’s Way, Huntingdon, Cambs, PE29 6EU, England

Telephone: 01480 454 528 / 451 499 / 451 980

Emergency: 999

Fax: 01480 456 031

Email: [email protected]

Trade/Type: Bright Cryospray 134 Aerosol Freezing Agent

Container: Aerosol

Uses: Rapid Freezing of tissue specimens to –52ºC Data Sheet: 48

Description: Colourless viscous liquid

Information on Ingredients Physical and Chemical Properties

1, 1, 1, 2 – Tetrafluoroethane – contents 80-100%

Appearance: Aerosol Odour: Characteristic

Hazards Identification: Stability and Reactivity

May cause frost bite if intentionally misused Avoid powdered metal, alkali metals alkali earth metals

Avoid heat, flames and other sources of ignition

First Aid procedures Toxicoloigical Information

Skin: Wash thoroughly, with soap and water May cause frost bite if intentionally misused No harmful

effect if handled correctly. May give off toxic fumes in the case of fire

Respiratory: Provide rest, warmth and fresh air If

discomfort continues, seek medical attention

Ingest: Rinse mouth out with water, in sever

cases seek medical attention

Eyes: Flush copiously for at least 15 minutes

SEEK MEDICAL ADVICE Fire Fighting Measures Ecological Information This product is not classed as flammable under current regulations. Special Fire Fighting Procedures : use water to keep fire exposed containers cool and disperse vapours. Breathing apparatus should be worn if exposure of fumes s likely. Unusual Fire Explosion Hazards: Possible risk of can rupture when exposed to fire/high temperatures. Hazardous Decomposition Products: Fire or high temperatures create halogenated hydrocarbons, oxides of carbon

Water Hazard Classification: Discharge of product will enter the atmosphere and will not result I long term aqueous contamination

Global Warming Potential: 0.28 (R11=1) (1,1,1,2 – Tetrafluorethane) Ozone Depletion Potential 0 (R11=1) (1,1,1,2 – Tetrafluorethane)

Accidental Release Measures Disposal Considerations

Spill: Let evaporate and ventilate area well Waste: DO NOT pierce or burn empty cans. Dispose

of in accordance with local authority requirements.

Handling and Storage Transport Information Usage Precautions: CAUTION pressurised container DO NOT expose to temperatures exceeding 50ºC DO NOT puncture or incinerate even when empty DO NOT spray onto naked flame or any incandescent

material Spray in short bursts to prevent cooling of the can STORAGE PRECAUTIONS: Store in a cool dry place, away from all sources of heat, including direct sunlight

Road: UN No.1950 CEFUC TEC (R) No.20G26-1 ADR Class 2 ADR ITEM No.5A Air: UN Air No.1950 Air Transport Class 2 Sea: UN Sea No.1950 Sea Transport Class No.2 IMDG Page No.2102

Exposure Controls Regulatory Information

1,1,1,2 – Tetrafluoroethane (HFC 134a) OED: Long term exp (8hours TWA ref period) 1000ppm (rec)/4240mg/m3 Skin: Avoid contact, it is advised to wear gloves Respiratory: Good ventilation required if used in confined space Ingest: Do not eat, drink or smoke Eyes: Wear goggles during use if there is any risk of eye contact, but not generally required under normal use.

CHIP: S23 DO NOT breathe gas/fumes/vapour/spray CHIP: S24/S25 Avoid contact with skin and eyes CHIP: S51 use only in well ventilated areas

COSHH Regulations 1999

Additional Information/Comments: Information given is, to the best of the Company’s knowledge and belief, accurate and reliable. However, no warranty, guarantee or representation is made to it’s accuracy, reliability of completeness.

Issue 7 /Jan 2004 - Last reviewed: Sept 2010 /Next review: Sept 2012

Page 25

M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

MATERIAL SAFETY DATA SHEET

Name: Low temperature Grease Part Number: 57355

Product Information

By: Bright Instrument Company Limited

Address: St Margaret’s Way, Huntingdon, Cambs, PE29 6EU, England

Telephone: 01480 454 528 / 451 499 / 451 980

Emergency: 999

Fax: 01480 456 031

Email: [email protected]

Trade/Type: OPTITEMP TTI

Container: Plastic Tube

Uses: For lubrication of microtomes and remote

control spindles Data Sheet: 87

Description: Lubrication at arctic to high temperature

ranges

Information on Ingredients Physical and Chemical Properties

Lubricant grease based on synthetic oil

Physical form: Paste Density: @20ºC, 965Kg/m3 Odour: Mild Colour: Brown Flashpoint: (closed) 215 (Mineral) Water Solubility: Insoluble

Hazards Identification: Stability and Reactivity

This product is NOT classified as hazardous Stable: Yes will not polymerise

Conditions to Avoid: Temperatures above 215ºC Materials to Avoid: Strong oxidising agents Hazardous Decomposition/Combustion Products: NONE with correct use.

First Aid procedures Toxicoloigical Information

Skin: Wash thoroughly, with soap and

water

Health effects:

EYES: May cause transient irritation. SKIN: Unlikely to irritate on brief or occasional exposure INHALATION: Low volatility make inhalation unlikely at ambient temperatures. INGESTION: May cause nausea, vomiting and diarrhoea CHRONIC: Repeated and prolonged skin contact may lead to skin disorders. OTHER: NONE known

Respiratory: Remove from exposure Ingest: DO NOT induce vomiting. Seek

medical advise immediately Eyes: Flush copiously for at least 15

minutes. If irritation persists

SEEK MEDICAL ADVISE

Fire Fighting Measures Ecological Information

FIRE: Extinguish fires with foam, dry

powder, CO2 or water fog - do not

use water jets

When used and disposed of as intended, no adverse environmental effects are foreseen

Accidental Release Measures Disposal Considerations

Spill: Avoid entry into drains and

waterways, spilt product will

present a slip hazard

Waste: Dispose of in accordance with local

authority requirements

Handling and Storage Transport Information

Handling: Storage:

No special precautions

Store out of direct sunlight, do not

leave container unsealed

Not classified as dangerous to transport

Exposure Controls Regulatory Information

None of the ingredients of this product have an occupational exposure limit

This product is a preparation and is NOT classified according to EEC Guideline 88/379

Issue 2 /March 2000 - Last reviewed: Sept 2010 / Next review: Sep 2012

Page 26

M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

Knives / Blades

SAFETY WARNING

EXTREMELY SHARP KNIVES / BLADES

USE KNIFE / BLADE GUARDS AT ALL TIMES

USE CORRECT TOOLS FOR REMOVAL AND

INSERTION OF KNIVES / BLADES

DO NOT LEAVE KNIVES / BLADES LAYING

AROUND

PLACE KNIVES / BLADES NOT IN USE, INTO

BOX / WALLET PROVIDED

Page 27

M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

QA028/ISSUE5/OCT00(F003)

HEALTH AND SAFETY AT WORK ACT

NTAMINATION CERTIFICATE

Any product which is to be returned to Bright Instrument Company Limited or serviced on site, must be cleaned and decontaminated in the appropriate manner. This certificate, duly completed, must be either sent in advance (fixed to the outer packing containing the product), or handed to the service engineer.

Packages will not be opened nor servicing commenced until the Company or service engineer have received a satisfactory certificate. Should returned goods be considered a hazard by the Company, they will be returned immediately to the customer at his/her expense. NB: Microtome knives must be in boxes.

Description:

Product Code: Serial Number:

Order Number: Quantity:

Tick Box A if applicable. Otherwise complete all parts of B, providing further information as requested or appropriate.

 This equipment has not been in contact with unfixed biological samples.

B 1. This equipment has been exposed internally or externally to hazardous materials as indicated below:

YES/NO Blood, body fluids, pathological samples Provide further details here: YES/NO Other biohazards YES/NO Chemicals/substances hazardous to health YES/NO Other hazards

2. This equipment has been cleaned and decontaminated:

YES/NO If YES, give details of the methods:

Provide further details here:

If NO*, please indicate why not:

* Such equipment must not be returned without the written agreement of Bright Instrument Company Limited.

3. The equipment has been prepared to ensure safe handling/transportation.

YES/NO

Signed: Institute:

Department:

Address:

Name: Postcode:

Position: Telephone: Extn:

Date: Facsimile:

Page 28

M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

DECONTAMINATION PROCEDURES

Cryostats & Ultra Low Temperature Freezing Units

If decontamination is required carry out the standard procedures as practised in your laboratory. It is the responsibility of the customer to use a decontamination procedure appropriate to his/her work. The following decontamination method is as recommended in the ‘Code of Practise for the Prevention of Infection in Clinical Laboratories and Post-mortem Rooms’, ISBN 0 11 320464 7.

1 Bring the cryostat to room temperature.

2 Place 50-100ml of formalin BP in a flat dish inside the chamber. Close the window.

3 Leave for at least 24 hours, preferably 48 hours.

4 Open the window and place a beaker containing 10ml of ammonia SG.880 in the chamber. Close

the window.

5 Leave for one hour. The cryostat is now decontaminated.

Microtomes

Microtome Knives

For further information regarding alternative decontamination procedures please refer to ‘Safe Working and the Prevention of Infection in Clinical Laboratories’, ISBN 0 11 885446 1.

Page 29

M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013

QA023/ISSUE 4/AUG99(F018)

QUALITY SURVEY REPORT

Our watchword is QUALITY. In our continuing endeavour to improve the quality and performance of our processes and products, we would welcome any initial comments on the following aspects of our service and products. As you have only just received the product we do not feel that you could asses the actual workings of the instrument accurately, so we will follow up in approximately six months with a Customer Feedback – Voice of the Customer questionnaire. If, of course, you have any comments to make prior to receiving the questionnaire, please feel free to contact us.

Please return this form either by post or by fax on 01480-456031, for the attention of the QA Manager.

Model: Serial Number:

Institute: Department:

Address:

Postcode:

Telephone: Extension:

Aspect Comments and Suggestions

Purchasing: Did the purchasing process

run smoothly with respect to our involvement? e.g. correct advice, lead times, payment arrangements etc.

Delivery: Was the instrument in a satisfactory condition on arrival?

Installation: Did we install the instrument? If so was adequate pre-use instruction given?

User information: Did you receive an operating manual? Do you believe it is comprehensive enough for your use?

Safety: Any comments?

Miscellaneous: Any other aspect you

would like to comment on, e.g. appearance, first impressions etc.

Signed:………………….…………….…. Name:………………………..….…..……. Position:……….……….……………..…….

Thank you for helping us to help you in the future

Page 30

EMOTORCIM 0053M

YLBMESSA

040-212

RAER NO WEIV

EDISREDNU MORF

20 YAM

JJT

3 FO 1 THS

TNORF NO WEIV THGIR POT MORF

ELDNAH EVIRD

DEVOMER

200-212 & 100-212 NO DESU TXEN YLBMESSA SIHT

SGNIWARD SIHT FO 3 & 2 STEEHS OT REFER SLIATED ROF

NWARD

DECART

DEKCEHCDEVORPPAETAD

EUSSI

NOITPIRCSED

TSIL STRAP EES -: LAIRETAM

NAELC -: HSINIF

2:1 ELACS

mm NI SNOISNEMID LLA

NOITCEJORP ELGNA dr3

.DTL .OC TNEMURTSNI THGIRB

ELTIT

.oN GNIWARD

DETATS SSELNU SECNARELOT

D'PPA

ETAD

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1732 NC

50/9

DEVRESER STHGIR LLA - DETCETORP THGIRYPOC

! ELACS TON OD

!KSA - TBUOD NI FI

-: LAMICED

-: soN ELOHW

-: RALUGNA

01,0 05,0

5,0

-: SETON .SEGDE DNA SRENROC PRAHS ,SRRUB LLA EVOMER .1

.EERF HCTARCS DNA NAELC EB OT SECAFRUS LLA .2

Page 31

EMOTORCIM 0053M

YLBMESSA

)

(

YLBMESSA-BUS EES

03325 oN TP OT

YLBMESSA -BUS EES

85915 oN TP OT

YLBMESSA-BUS EES

140-212 oN TP OT

YSSA BUS MRA NIAM

73915 oN TP EES

WEIV 'DEDOLPXE' ROF

YLBMESSA-BUS EES

160-212 oN TP OT

YLBMESSA -BUS EES

150-212 oN TP OT

TCARTER EES

YLBMESSA-BUS

450-212 oN TP OT

040-212

JJT

20 YAM

3 FO 2 THS

GNIWARD SIHT FO 1 TEEHS EES YSSA TXEN ROF

SIHT NI SMETI FO SLIATED ROF

3 TEEHS EES AERA

SIHT NI SMETI FO SLIATED ROF

3 TEEHS EES AERA

SIHT NI SMETI FO SLIATED ROF

3 TEEHS EES AERA

4,11

4222 NC

JJT

50,5

JJT

0032 NC

60,3

JJT

1732 NC DEWEIVER

NWARD

DECART

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NOITPIRCSED

TSIL STRAP TNEVELER EES -:LAIRETAM

NAELC -: HSINIF

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mm NI SNOISNEMID LLA

NOITCEJORP ELGNA dr3

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ELTIT

.oN GNIWARD

DETATS SSELNU SECNARELOT

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ETAD

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! ELACS TON OD

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-: LAMICED

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01,0 05,0

5,0

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.EERF HCTARCS DNA NAELC EB OT SECAFRUS LLA .2

Page 32

SNOITISOP EERHT NI

.EDIS HCAE

SNSOP 3 NI

SNSOP OWT NI

10502 & 9402 NC ,GNIWARD WEN

JJT

20,5

9405

918

3323

5383

40,11

JJT

.SON METI WEN HTIW NWD'R 4222 NC

50,5JJT

0032 NC

60,3

JJT

1732NC

NWARD

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DEVORPPA

ETAD

.DTL .OC TNEMURTSNI THGIRB

JJT

3 FO 3 THS

EMOTORCIM 0053M

YLBMESSA

.oN GNIWARD

040-212

SETON

20.5.31

DEVRESER STHGIR LLA-DETCETORP THGIRYPOC

ELTIT

.SEHCTARCS MORF EERF DNA NAELC EB OT SECAFRUS LLA .1 .SEGDE PRAHS DNA SRENROC PRAHS ,SRRUB LLA EVOMER .2

.DETATS ESIWREHTO SSELNU SECNARELOT

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GWD SIHT F0 2 THS -:ON YSSA NO DESU TSRIF

TSIL STRAP EES :LAIRETAM

NAELC :HSINIF

5,2:1 ELACS

mm NI SNOISNEMID LLA

.NOITCEJORP ELGNA dr3

Page 33

Part List

ReferenceItem

ode

212-040 ** ASSEMBLY, M3500MICROTOME

-PL

11 M3500 BASE CASTING RETRACT 51901-1 12 M3500 REWIND SHAFT 212-445 13 THICKNESS DISC LABEL SET 212-811 24 3020 REWIND SHAFT BUSH 51951 15 3020 REWIND SHAFT COLLAR 51952 26 PTFE WASHER (REWIND) 52013 17 M3500 THICKNESS DIAL HUB 212-411 28 THICKNESS CAM BUSH 50818 1 in 519149 3020 MAIN SHAFT 51909 210 3020 OIL RETAINING RING 52210 211 TAPER ROLLER RACE 32004XA 51990 1 in 5191412 3020 REWIND PULLEY ASSEMBLY 51958 1 in 5191413 3020 DRIVE ARM 51910 114 3020 THICKNESS CAM 51938 115 3020 REWIND SHAFT PULLEY ASSY 52330 1 in 5191416 CLINICUT/ STARLET TAPER PIN 51911 1 in 5191417 M5 X 10 SKT CAP HD ST/ST 55105 218 3T5/255 TIMING BELT 56563 220 CAM PLATFORM BUSH 51945-2 221 CAM PLATFORM BUSH 51945-1 222 CAM PLATFORM SPRING 51993 623 M4 x 4 SOCKET GRUB SCREW BZP 55000 124 M3500 CRANKSHAFT 212-479 125 3020 INPUT DRIVE GEAR 51904 226 BALL RACE BEARING 50991 127 M3500 CRANKSHAFT CAM 212-474 128 M3500 CRANKSHAFT CONNECTING SCREW 212-463 2 in 5233429 M3500/3020 CON ROD END 52334 130 3500/3020 KNOCK OFF CAM 52011 131 3020 CAM PLATFORM 51943 232 CAM PLATFORM PILLAR 51944 433 M3 X 5 GRUB SCREW BZP 50947 134 BALL (5/32")ST/ST =(4MM) 50956 135 3020 DEBRIS ROLLER SUPPORT LH 52117 636 3020 DEBRIS ROLLER BEARING 52120 137 3020 DEBRIS ROLLER SUPPORT RH 52118 338 3020 DEBRIS BLIND ROLLER 51997 439 M3 x 10 SKT CAP HD ST/ST 55241 1 in 5190840 3020 CON ROD LINK 51907 141 M3500 DRIVE ARM SCREW 212-462 242 3020 DEBRIS ROLLER SUPPORT 3M 52119 243 M3 X 12 SKT CAP HD ST/ST 55120

Page 1 of 2

ISSUE CHECKED APPROVED AUTHORISED DATE

3 TJJ DJS CN 2371 07/03/2006

Page 34

Part List Continued

ReferenceItem

ode

212-040 ** ASSEMBLY, M3500MICROTOME

-PL

145 3020 DEBRIS ROLLER SUPP LH 51996 146 3020 DEBRIS ROLLER SUPP RH 51995 547 M4 X 10 SKT CAP HD ST/ST 55100 1 in 5191448 M4 PLAIN WASHER ST/ST 55616 1 in 5191449 M4 HEX FULLNUT ST/ST 55514 250 M5 X 6 GRUB SCREW BZP 55006 151 E CLIP 5/16" BZP 56403 152 M3500 FRONT COVER RIGHT BRACKET 212-104 153 M3500 FRONT COVER LEFT BRACKET 212-105 654 M4 x 8 PAN HD POZI ST/ST 55239 155 PLUNGER BALL 6MM 51341 156 M3500 PAWL RELEASE KNOCK OFF PIN 212-450 257 M3500 LONG MICROTOME STUD 212-416 258 M3500 SHORT MICROTOME STUD 212-417 159 3020 DEBRIS BLIND SUPP PIN 51999 160 PAWL ARM EXTENSION SPRING 58274 161 DEBRIS BLIND EXTENSION SPRING 58275 162 OILITE TIE BUSH 50532 168 ** M3500, MOTOR REWIND ASSEMBLY 212-041 169 ** M3500, PAWL RELEASE ASSEMBLY 212-051 170 ** M3500 KNIFE BLOCK RETRACT ASSY 212-054 172 ** M3500, INPUT DRIVESHAFT

(HANDWHEEL) ASSEMBLY

212-061

Page 2 of 2

ISSUE CHECKED APPROVED AUTHORISED DATE

3 TJJ DJS CN 2371 07/03/2006

Page 35

YLBMESSA MRA GNITTUC

0053M & 0203

73915

4.1732 NC

JJT

60,2

JJT

YLBMESSA MRA GNITTUC

6,3,6

NWARD

DECART

DEKCEHCDEVORPPAETAD

EUSSI

NOITPIRCSED

TSIL STRAP EES :LAIRETAM

NAELC :HSINIF

2:1 ELACS

mm NI SNOISNEMID LLA

NOITCEJORP ELGNA dr3

.DTL .OC TNEMURTSNI THGIRB

ELTIT

.oN GNIWARD

DETATS SSELNU SECNARELOT

D'PPAETAD

DEVRESER STHGIR LLA - DETCETORP THGIRYPOC

! ELACS TON OD

!KSA - TBUOD NI FI

-: SETON .SEGDE DNA SRENROC PRAHS ,SRRUB LLA EVOMER .1

.EERF HCTARCS DNA NAELC EB OT SECAFRUS LLA .2

-: LAMICED

-: soN ELOHW

-: RALUGNA

01,0 05,0

5,0

Page 36

Part List

ReferenceItem

ode

51937 3020 MAIN ARM ASSEMBLY

-PL

** Use Assembly Drawing 51937 **

11 3020 RATCHET WHEEL SCREW 51923 42 M3 X 6 SOCKET CAP HEAD ST/ST 50952 1 3-11/21/313 3020 PAWL SCREW 51928 14 3020 PAWL PLASTIC 51927 15 3020 PAWL SPACER 51929 16 5040/5030 PAWL TENSION SPRING 50414 17 CLINICUT CAM ROLLER SPACER 51933 18 ANTI-ROLL MICRO BEARING 51270 19 3020 CAM ROLLER SCREW 51932 110 PAWL ARM SPRING 51992 111 3020 SPRING PLATE 51935 112 M3500/3020 LEADSCREW BODY 2 TO 30 51917 113 M3500/3020 LEADSCREW 2 TO 30 51918 114 3020 MAIN ARM KEY 52086 215 THRUST RACE SINGLE SKF 51100 51989 216 3020 MAIN ARM SCREW 51936 117 3020 MAIN ARM MACHINED 51916 118 M4 X 16 SKT CAP HD ST/ST 55104 119 3020 PAWL STOP PIN 51925 120 3020 PAWL ARM 51919 221 M3 X 6 CSK SLTD HEAD ST/ST 51550 122 3020 PAWL ARM RETAINER 51920 123 CRINKLE WASHER M12 51233 124 M3500 RATCHET WHEEL 212-432 325 M4 X 6 GRUB SCREW BZP 55001 126 M3500 RATCHET WHEEL PULLEY 212-433 127 3020 RATCHET PULLEY PLATE 51924 228 M3 x 8 SKT CAP HD ST/ST 55101 229 M4 X 5 SOCKET SET SCREWS ST/ST 55037 130 M3 PLAIN WASHER ST/ST MAX OD 7mm 55610 131 M5 X 5 GRUB SCREW BZP 50958

Page 1 of 1

ISSUE CHECKED APPROVED AUTHORISED DATE

4 TJJ DJS CN 2371 06/03/2006

Page 37

140-212

EMOTORCIM

DNIWER ROTOM

YLBMESSA BUS

JJT20/5/2

NWARD

DECART

DEKCEHCDEVORPPAETAD

EUSSI

NOITPIRCSED

TSIL STRAP EES -:LAIRETAM

NAELC :HSINIF

1:1 ELACS

mm NI SNOISNEMID LLA

NOITCEJORP ELGNA dr3

EMOTORCIM

DNIWER ROTOM

YLBMESSA-BUS

.DTL .OC TNEMURTSNI THGIRB

ELTIT

.oN GNIWARD

DETATS SSELNU SECNARELOT

D'PPA

ETAD

60,2

JJT

DOM1732 NC .

20,5

JJT

0502 NC .GRD WEN

DEVRESER STHGIR LLA - DETCETORP THGIRYPOC

! ELACS TON OD

!KSA - TBUOD NI FI

-: LAMICED

-: soN ELOHW

-: RALUGNA

01,0 05,0

5,0

-: SETON .SEGDE DNA SRENROC PRAHS ,SRRUB LLA EVOMER .1

.EERF HCTARCS DNA NAELC EB OT SECAFRUS LLA .2

Page 38

Part List

ReferenceItem

ode

212-041 ** M3500, MOTOR REWIND ASSEMBLY

-PL

11 3020 MOTOR BRACKET 52534-1 12 STEPPER MOTOR ADV/REW 138103-01 13 M3500 / 3020 MOTOR REWIND COVER 52159 14 OLDHAM COUPLING 6.00MM BORE 50434-03 15 OLDHAM COUPLING NYLON DISC 50434-04 16 OLDHAM COUPLING 1/4" BORE 50434-01 17 V-RING SEAL V6A 52158 18 ** M3500 MOTOR LOCATOR 52550-1 49 M5 X 12 PAN HD POZI BZP 55219 510 M4 X 8 SKT CAP HD ST/ST 55033 511 M4 PLAIN WASHER ST/ST 55616 312 M4 NYLON WASHER 55633

Page 1 of 1

ISSUE CHECKED APPROVED AUTHORISED DATE

1 TJJ DJS CN2371 17/02/2006

Page 39

1732NC . NWARDERJJT

60,3

GNITATNEIRO -NON SRQC

YLBMESSA

GNITATNEIRO -NON SRQC

YLBMESSA

NWARD

DECARTDEKCEHC

DEVORPPA

ETAD

.DTL .OC TNEMURTSNI THGIRB

JJT

FO THS

.oN GNIWARD

640 - 212

SETON

20/01/82

DEVRESER STHGIR LLA-DETCETORP THGIRYPOC

ELTIT

.SEHCTARCS MORF EERF DNA NAELC EB OT SECAFRUS LLA .1 .SEGDE PRAHS DNA SRENROC PRAHS ,SRRUB LLA EVOMER .2

.DETATS ESIWREHTO SSELNU SECNARELOT

LAMICED

.oN ELOHW

.RALUGNA

01,0

05,0

5,0

EUSSI

NOITPIRCSEDD'PPAETAD

!ELACS TON OD

!KSA - TBUOD NI FI

-:ON YSSA NO DESU TSRIF LIATED SIHT

mm NI SNOISNEMID LLA

.NOITCEJORP ELGNA dr3

Page 40

Part List

ReferenceItem

ode

212-046 M3500 HOLDER CASSETTE QUICK RELEASE

-PL

11 M3500 CQRS BODY 212-434 12 M3500 CQRS LOWER CLAMP 212-435 13 M3500 CQRS UPPER CLAMP 212-436 14 M3500 CQRS SUPPORT CLAMP 212-437 15 M3500 CQRS KNOB 212-438 16 M3500 CQRS NON ORIENTATING ADAPTOR 212-439 17 M3500 CQRS HANDLE 212-440 18 M3500 CQRS RELEASE PIN 212-441 19 M3500 CQRS RELEASE PIN SLEEVE 212-442 110 CQRS RETAINING PIN 50697 211 SPRING FOR CQRS 52657 112 M3500 COMPRESSION SPRING CQRS 58173 213 M4 X 16 SKT CAP HD ST/ST 55104 214 M3 x 8 SKT CAP HD ST/ST 55101 115 M5 X 5 GRUB SCREW BZP 55004 416 M5 X 12 GRUB SCREW BZP 55007 217 M4 x 4 SOCKET GRUB SCREW BZP 55000 118 M6 X 6 GRUB SCREW BZP 55008

Page 1 of 1

ISSUE CHECKED APPROVED AUTHORISED DATE

2 TJJ DJS CN2371 07/03/2006

Page 41

SNSOP 2 NI

PMALC GNITATNEIRO

- YLBMESSA BUS REBMUN TRAP OT

100-740-212

0502NC .GNIWARD WEN

YLBMESSA DAEH GNITATNEIRO SRQC

0053M

SRQC

GNITATNEIRO

YLBMESSA

JJT

20,01

NWARD

DECARTDEKCEHC

DEVORPPA

ETAD

.DTL .OC TNEMURTSNI THGIRB

JJT

FO THS

.oN GNIWARD

740 - 212

SETON

20/01/82

DEVRESER STHGIR LLA-DETCETORP THGIRYPOC

ELTIT

.SEHCTARCS MORF EERF DNA NAELC EB OT SECAFRUS LLA .1 .SEGDE PRAHS DNA SRENROC PRAHS ,SRRUB LLA EVOMER .2

.DETATS ESIWREHTO SSELNU SECNARELOT

LAMICED

.oN ELOHW

.RALUGNA

01,0

05,0

5,0

EUSSI

NOITPIRCSEDD'PPAETAD

!ELACS TON OD

!KSA - TBUOD NI FI

-:ON YSSA NO DESU TSRIF LIATED SIHT

TSIL STRAP EES :LAIRETAM

NAELC : HSINIF

1:1 ELACS

mm NI SNOISNEMID LLA

.NOITCEJORP ELGNA dr3

Page 42

Part List

ReferenceItem

ode

212-047 M3500 QUICK RELEASE CASETTE

-PL

11 M3500 CQRS BODY 212-434 12 M3500 CQRS LOWER CLAMP 212-435 13 M3500 CQRS UPPER CLAMP 212-436 14 M3500 CQRS SUPPORT CLAMP 212-437 15 M3500 CQRS KNOB 212-438 16 M3500 CQRS HANDLE 212-440 17 COMPRESSION SPRING M3500 58175 18 M3500 ORIENTATING HEAD REAR CLAMP 212-456 19 M3500 ORIENTATING HEAD FRONT CLAMP 212-457 110 M3500 CQRS ORIENTATING RELEASE PIN 212-460 111 M3500 CQRS ORIENTATING ADAPTOR 212-459 112 CQRS RETAINING PIN 50697 213 SPRING FOR CQRS 52657 214 M4 X 16 SKT CAP HD ST/ST 55104 415 M3 x 8 SKT CAP HD ST/ST 55101 116 M5 X 5 GRUB SCREW BZP 55004 417 M5 X 12 GRUB SCREW BZP 55007 218 M4 x 4 SOCKET GRUB SCREW BZP 55000 120 ORIENT OBJECT HOLDER BALL 51983 121 ORIENT OH LOCK LEVER 52097 122 3020 ORIENT OH LOCK SCREW 52261 123 3020 ORIENT OH LOCK 52260 124 3020 ORIENT OH LOCK KNOB 52098 225 M3 X 5 GRUB SCREW BZP 50947 126 1/16 X 1/4 SPRINGPIN ST/ST 55904

Page 1 of 1

ISSUE CHECKED APPROVED AUTHORISED DATE

2 TJJ DJS CN2371 07/03/2006

Page 43

YSSA ESAELER LWAP

150 - 212

YSSA ESAELER LWAP

0502 NC ,EUSSI WENJJT

20/01

20/5

JJT

20/5

JJT

NWARD

DECART

DEKCEHCDEVORPPAETAD

EUSSI

NOITPIRCSED

TSIL STRAP EES -:LAIRETAM

NAELC -:HSINIF

1:1 ELACS

mm NI SNOISNEMID LLA

NOITCEJORP ELGNA dr3

.DTL .OC TNEMURTSNI THGIRB

ELTIT

.oN GNIWARD

DETATS SSELNU SECNARELOT

D'PPA

ETAD

DEVRESER STHGIR LLA - DETCETORP THGIRYPOC

! ELACS TON OD

!KSA - TBUOD NI FI

-: LAMICED

-: soN ELOHW

-: RALUGNA

01,0 05,0

5,0

-: SETON .SEGDE DNA SRENROC PRAHS ,SRRUB LLA EVOMER .1

.EERF HCTARCS DNA NAELC EB OT SECAFRUS LLA .2

Page 44

Part List

ReferenceItem

ode

212-051 ** M3500, PAWL RELEASE ASSEMBLY

-PL

11 M3500 PAWL RELEASE BRACKET 212-447 12 M3500 PAWL RELEASE LEVER LONG 212-448 13 M3500 PAWL RELEASE LEVER SHORT 212-449 14 M3500 PAWL RELEASE KNOCK OFF PIN 212-450 15 M3500 PAWL RELEASE PIVOT PIN 212-451 26 M3500 PAWL RELEASE SPRING HOLDER 212-452 27 M3500 PAWL RELEASE LEVER BOSS 212-453 18 PAWL ARM SPRING 51992 19 M4 X 12 SKT CAP HD BZP 55103 110 M3 X 12 SKT CAP HD ST/ST 55120 211 M4 x 4 SOCKET GRUB SCREW BZP 55000 112 M3 x 8 SKT CAP HD ST/ST 55101 113 M3 FULL NUT ST/ST 50942

Page 1 of 1

ISSUE CHECKED APPROVED AUTHORISED DATE

1 TJJ DJS CN2371 20/02/2006

Page 45

KCOLB EFINK 0203 / 0053 M

LEDOM TCARTER

YLBMESSA - BUS

NI NWOHS SA 2

SNOITISOP 2

NWOHS SA 2

SNSOP 2 NI

SNOITISOP 2

MORF DNA HTAENEB MORF NWOHS SI YLBMESSA

.TINU FO RAER EHT

1 METI DENIHCAM ) ESAB EMOTORCIM ( GNITSAC NIAM EHT

.YTIRALC NI TSISSA OT DETTIMO NEEB SAH TSIL STRAP FO

DETCURTSNOC ,ESIWREHTO

EDIS HR REP SA

SIHT DERIUQER METI

DNAH THGIR NI

YLNO KCOLB EFINK

8732 NC .DEIFIDOM

JJT

60,4

20,01

3602 NC .GNIWARD WEN

JJT

2332 NC & 0332 NC .DEIFIDOM

JJT

50.90

4922 NC .DEIFIDOM

JJT

50.50

TSIL STRAP EES :LAIRETAM

NAELC :HSINIF

1:1 ELACS

NWARD

DECART

DEKCEHC

DEVORPPA

ETAD

.DTL .OC TNEMURTSNI THGIRB

JJT

FO THS

.oN GNIWARD

450 - 212

SETON

20,01,9

DEVRESER STHGIR LLA-DETCETORP THGIRYPOC

ELTIT

.SEHCTARCS MORF EERF DNA NAELC EB OT SECAFRUS LLA .1

.SEGDE PRAHS DNA SRENROC PRAHS ,SRRUB LLA EVOMER .2

.DETATS ESIWREHTO SSELNU SECNARELOT

LAMICED

.oN ELOHW

.RALUGNA

01,0

05,0

5,0

EUSSI

NOITPIRCSEDD'PPAETAD

!ELACS TON OD

!KSA - TBUOD NI FI

-:ON YSSA NO DESU TSRIF LIATED SIHT

mm NI SNOISNEMID LLA

.NOITCEJORP ELGNA dr3

Page 46

Part List

ReferenceItem

ode

212-054 ** M3500 KNIFE BLOCK RETRACT ASSY

-PL

11 M3500 BASE CASTING RETRACT 51901-1 12 M3500 KNIFE BLOCK LH RETRACT 212-466 13 M3500 KNIFE BLOCK RH RETRACT 212-467 24 M3500 PIVOT BAR KNIFE BLOCK RETRACT 212-468 15 M3500 KNIFE BLOCK RETRACT LEVER LH 212-469 16 M3500 KNIFE BLOCK RETRACT LEVER RH 212-470 27 M3500 CONNECT ROD HOLDER 212-471 28 M3500 ADJUSTER SCREW PAD 212-472 29 ** M4 X 8 PTD GRUB SCREW BZP 55002-1 110 M3500 KNIFE BLOCK RETRACT PUSH ROD

PAD

212-481

111 M3500 PUSH ROD 212-482 112 M3500 PUSH ROD LOCATION PIN 212-473 113 M3500 CRANKSHAFT 212-479 114 M3500 CRANKSHAFT CAM 212-474 415 M3500 SPRING HOLDER 212-475 116 M3500 CONNECT ROD 212-476 217 M3500 OILITE BUSH 212-477 118 M3500 CONNECT ROD SUPPORT 212-478 419 TENSION SPRING 58135 120 MICRO BEARING DOUBLE SHIELDED 50940 121 ST/ST DOWEL PIN 2.5mm x 8mm 50489-1 422 M3 X 12 SKT CAP HD ST/ST 55120 423 M4 X 10 SKT CAP HD ST/ST 55100 624 M4 X 12 GRUB SCREW BZP 55003 225 M4 x 4 SOCKET GRUB SCREW BZP 55000 226 M4 x 6 SOCKET HEAD C/SUNK ST/ST 55030 127 3020 KNIFE CLAMP LH 52234 128 3020 KNIFE CLAMP RH 52235 129 TIE BAR 50442 230 5040/3020 KNIFE CLAMP SCREW 52195 231 LOCKING KEY KNOB 53779 132 M3500 WAX KNIFE GUARD BRACKET RH 212-426 233 M3500 WAX KNIFE GUARD HOLDER 212-428 234 M3500 KNIFE GUARD ROD 212-429 235 M3500 KNIFE GUARD HANDLE 212-430 236 M3500 KNIFE GUARD STUD 212-431 237 M4 KNOB 51542 238 M5 X 5 GRUB SCREW BZP 50958 241 PLUNGER BALL 4mm 51443 242 M3 X 6 CSK SLTD HEAD ST/ST 51550 243 M3 X 6 SOCKET CAP HEAD ST/ST 50952

Page 1 of 2

ISSUE CHECKED APPROVED AUTHORISED DATE

4 TJJ DJS CN2378 08/05/2006

Page 47

Part List Continued

ReferenceItem

ode

212-054 ** M3500 KNIFE BLOCK RETRACT ASSY

-PL

244 005X1/8 HAMMER DRIVE BRASS 56100 245 3020 KNIFE GUARD KNOB 52244 246 M6 x ID F/R COILTHREAD WIRE INSERT 55005 147 M3500 WAX KNIFE GUARD BRACKET L H 212-427 148 5040 KNIFE BLOCK POINTER 50526 149 PLUNGER BALL 6MM 51341

Page 2 of 2

ISSUE CHECKED APPROVED AUTHORISED DATE

4 TJJ DJS CN2378 08/05/2006

Page 48

TFAHS EVIRD TUPNI

YSSA )LEEHWDNAH(

0053M

160-212

JJT

2/5/2

-: SETON .SEGDE DNA SRENROC PRAHS ,SRRUB LLA EVOMER .1

.EERF HCTARCS DNA NAELC EB OT SECAFRUS LLA .2

.YSSA LANIF TA DETTIF EB YAM EVISULCNI NEVES OT 1 SMETI .3

EB OT DEEN LLIW SMETI ESEHT , REILRAE DETTIF FI

REVOC GNINOITISOP RETFA DETTIFER DNA DETNUOMSID

2,5,2

JJT

0502 NC

NWARD

DECART

DEKCEHCDEVORPPAETAD

EUSSI

NOITPIRCSED

TSIL STRAP EES : LAIRETAM

NAELC : HSINIF

2:1 ELACS

mm NI SNOISNEMID LLA

NOITCEJORP ELGNA dr3

.DTL .OC TNEMURTSNI THGIRB

ELTIT

.oN GNIWARD

DETATS SSELNU SECNARELOT

D'PPA

ETAD

DEVRESER STHGIR LLA - DETCETORP THGIRYPOC

! ELACS TON OD

!KSA - TBUOD NI FI

-: LAMICED

-: soN ELOHW

-: RALUGNA

01,0 05,0

5,0

Page 49

Part List

ReferenceItem

ode

212-061 ** M3500, INPUT DRIVESHAFT (HANDWHEEL)

ASSEMBLY

-PL

11 M3500/OTF5000 H/ WHEEL CASTING 212-403 12 HANDWHEEL TRIGGER 50033 13 M6 X 12 ROOFING BOLT BZP 55701 14 M3 x 10 PAN POZI ST/ST 55240 15 HANDWHEEL TRIGGER SPRING 50530 16 BALL GRIP P111/47+X-M10 52252 17 M3500/OTF5000 HANDWHEEL SCREW 212-413 18 M3500 HANDWHEEL BUSH HOLDER 212-423 19 M3500 STOP RING HOLDER 212-422 110 STOP RING 50622 311 M4 X 30 SOCKET CAP HEAD ST/ST 55099 312 M4 X 16 SKT CAP HD ST/ST 55104 213 M4 X 10 CSK HD SLTD BZP 55212 114 M3500 HANDWHEEL SHAFT 212-421 115 PEG TOP SHAFT CUTTING HANDWHEEL 50593 116 E CLIP 1/2" BZP 56402 217 PTFE WASHER 3/8" 3020 52083 118 5040 DRIVE SPINDLE BUSH 51058 219 PTFE WASHER DRIVE 1/2" 52014 220 M3500 HANDWHEEL SHAFT SPACER 212-424 221 BALL RACE BEARING 50991 122 3020 BEARING SPACER 51903 123 3020 INPUT DRIVE GEAR 51904 324 M4 x 4 SOCKET GRUB SCREW BZP 55000 225 M4 X 6 GRUB SCREW BZP 55001

Page 1 of 1

ISSUE CHECKED APPROVED AUTHORISED DATE

3 TJJ DJS CN2371 20/02/2006